Rh5CG201700

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003409
Physical Location & Seq
Forward (+)
20242159 .. 20245236
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG201700.1

Sequence Viewer

Length: 615 bp
ATGGAGCGCAAAGACAATCAGTCTCCTCAAGATGAAACCGCTCGGATTCTGGTCGAGTTCTTGGAGGTGGCAATCACTTCAATCGTTTTCCTCAAAGGAATTTACCCCCCAGGTGCTTTTGAGAGAAGGAAGTACATGAATTTGGTGGTTCATAGAGCTCGGCACCCTGAGCTTAGAGATTATATCCATTCTGCTGTCTCAGGACTTCTTCCTTTTATCAAAAAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTTGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACGTCAAAGAAGCTGACCTAGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAATTGCAGGTGGGAGATAACGGCATACTTCCGGACCCTTCCTCAGGAAATTTCAAGCAAGAATGAAGGGTTGTGGCTTCCAACAGACACAAAACAGTGGCAACAACCTCCCCTAATAACTCCTATTAAGTCAATGAGTAGCCAACCTCTCTCTGTGCAGTTGTATCTAGAACATCCAAGTGTATATGAACCAAAGCCGTGA

Protein Analysis

204

Amino Acids

23.6

Weight (kDa)

7.76

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HORMA PF02301 18 - 151 1.6e-09 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 2 cut(s) 39, 393
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 3 cut(s) 99, 139, 462
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 2 cut(s) 81, 468
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 158, 172, 298, 338, 376
AluI AGCT 5 cut(s) 158, 172, 298, 338, 376
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 27, 202
AlwI GGATC 1 cut(s) 315
Aor13HI TCCGGA 1 cut(s) 444
ApoI RAATTY 3 cut(s) 99, 139, 462
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 447
AvaII GGWCC 1 cut(s) 447
AxyI CCTNAGG 1 cut(s) 456
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 160
BceAI ACGGC 2 cut(s) 450, 595
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 27, 202
BfaI CTAG 2 cut(s) 344, 581
BfuAI ACCTGC 1 cut(s) 411
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 447
BmeRI GACNNNNNGTC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 164, 449
BmrFI CCNGG 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse21I CCTNAGG 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 444
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 4 cut(s) 159, 213, 425, 470
BseRI GAGGAG 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 445
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 27, 202
Bsp1286I GDGCHC 1 cut(s) 160
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 39, 393
BspCNI CTCAG 4 cut(s) 160, 212, 424, 469
BspEI TCCGGA 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 164, 449
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 3 cut(s) 263, 304, 510
BstDEI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 2 cut(s) 27, 202
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 456
BtsCI GGATG 1 cut(s) 586
BtsIMutI CAGTG 3 cut(s) 268, 300, 515
BveI ACCTGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 447
Csp6I GTAC 1 cut(s) 133
CviAII CATG 1 cut(s) 136
CviJI RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
CviKI_1 RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 160
FaeI CATG 1 cut(s) 139
FaiI YATR 9 cut(s) 137, 153, 183, 278, 290, 386, 439, 598, 600
FatI CATG 1 cut(s) 135
FokI GGATG 1 cut(s) 573
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 2 cut(s) 344, 581
GlaI GCGC 1 cut(s) 8
HapII CCGG 1 cut(s) 445
HhaI GCGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 46, 389
HpaII CCGG 1 cut(s) 445
Hpy188I TCNGA 2 cut(s) 45, 414
Hpy188III TCNNGA 5 cut(s) 29, 201, 445, 458, 581
HpyAV CCTTC 3 cut(s) 120, 461, 473
HpyCH4III ACNGT 3 cut(s) 263, 304, 510
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 420, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 1 cut(s) 7
Kpn2I TCCGGA 1 cut(s) 444
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 35, 96, 123, 180, 186, 215, 406, 443, 458
MaeI CTAG 2 cut(s) 344, 581
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 263
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 200, 398
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 4 cut(s) 99, 139, 415, 462
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 8 cut(s) 36, 58, 101, 390, 420, 465, 531, 570
MroI TCCGGA 1 cut(s) 444
MseI TTAA 2 cut(s) 294, 540
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 169
NdeII GATC 1 cut(s) 307
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 2 cut(s) 164, 449
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 46, 389
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 164, 449
PspPI GGNCC 1 cut(s) 447
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 2 cut(s) 294, 540
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 447
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 160
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 4 cut(s) 99, 139, 415, 462
SsiI CCGC 2 cut(s) 39, 393
SspMI CTAG 2 cut(s) 344, 581
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 3 cut(s) 263, 304, 510
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 54
TasI AATT 4 cut(s) 99, 139, 415, 462
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 46, 389
Tru1I TTAA 2 cut(s) 294, 540
Tru9I TTAA 2 cut(s) 294, 540
TscAI CASTG 3 cut(s) 268, 307, 515
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 48, 140, 152, 492, 615
TspRI CASTG 3 cut(s) 268, 307, 515
VpaK11BI GGWCC 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 3 cut(s) 99, 139, 462
XbaI TCTAGA 1 cut(s) 580
XspI CTAG 2 cut(s) 344, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.