Rroxscaffold_1G00053030

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003409
Physical Location & Seq
Reverse (-)
73860617 .. 73863832
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053030.1

Sequence Viewer

Length: 525 bp
ATGGAGCGCAAAGACAATCAGTCTCCTCAAGGTGCTTTTGAGAGAAGGAAGTACATGAATTTGGTGGTTCATAGAGCTCGGCACCCTGAGCTCAGAGATTATATCCGTTCTGCTGTCTCAGGACTTCTTCCTTTTATCAAAAAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTCGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACATCAAAGAAGCTGACCTGGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAACCCCAGGGTTCCAATGGAAACAATTAAGTTCATTGAATCTAAATTGGATTTGCCGAATGACTTCAAGAAGTCACTGATTCGAAAGTACCCTGACTTTTTTGCAGTTAAAGATGTCAATGGAAGAGCTTATCTTCATTTGGAGAATTGGGACTCTTCACTAGCGGTCGCAGCTTGTGGAACGATTGGCTAA

Protein Analysis

174

Amino Acids

20.03

Weight (kDa)

9.25

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 111 - 169 7.6e-13 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 81
AciI CCGC 2 cut(s) 312, 497
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 1 cut(s) 58
AfaI GTAC 2 cut(s) 53, 422
AgsI TTSAA 2 cut(s) 371, 400
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 2 cut(s) 261, 338
AluBI AGCT 7 cut(s) 77, 91, 217, 257, 295, 461, 506
AluI AGCT 7 cut(s) 77, 91, 217, 257, 295, 461, 506
Alw21I GWGCWC 2 cut(s) 79, 93
Alw26I GTCTC 2 cut(s) 27, 121
AlwI GGATC 1 cut(s) 234
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 9
AsuII TTCGAA 1 cut(s) 415
BanI GGYRCC 1 cut(s) 81
BanII GRGCYC 2 cut(s) 79, 93
Bbv12I GWGCWC 2 cut(s) 79, 93
BbvI GCAGC 1 cut(s) 515
BciT130I CCWGG 2 cut(s) 263, 340
BcoDI GTCTC 2 cut(s) 27, 121
BfaI CTAG 1 cut(s) 494
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
Bme1390I CCNGG 2 cut(s) 263, 340
BmeRI GACNNNNNGTC 1 cut(s) 19
BmiI GGNNCC 2 cut(s) 83, 345
BmrFI CCNGG 2 cut(s) 263, 340
BpmI CTGGAG 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 338, 339
BseBI CCWGG 2 cut(s) 263, 340
BseDI CCNNGG 2 cut(s) 338, 339
BseMII CTCAG 4 cut(s) 78, 106, 132, 344
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 501
BshNI GGYRCC 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 79, 93
BslFI GGGAC 1 cut(s) 497
BsmAI GTCTC 2 cut(s) 27, 121
BsmFI GGGAC 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 79, 93
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 2 cut(s) 312, 497
BspCNI CTCAG 4 cut(s) 79, 105, 131, 343
BspLI GGNNCC 2 cut(s) 83, 345
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 451
BspT104I TTCGAA 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 338, 339
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 2 cut(s) 263, 340
Bst4CI ACNGT 2 cut(s) 182, 223
Bst6I CTCTTC 2 cut(s) 451, 493
BstBI TTCGAA 1 cut(s) 415
BstDEI CTNAG 6 cut(s) 87, 92, 118, 275, 318, 330
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 2 cut(s) 27, 121
BstMBI GATC 1 cut(s) 226
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 2 cut(s) 88, 503
BstNI CCWGG 2 cut(s) 263, 340
BstSCI CCNGG 2 cut(s) 261, 338
BstV1I GCAGC 1 cut(s) 515
BtsIMutI CAGTG 3 cut(s) 187, 219, 407
CfoI GCGC 1 cut(s) 9
Csp6I GTAC 2 cut(s) 52, 421
CviAII CATG 1 cut(s) 55
CviJI RGCY 9 cut(s) 77, 91, 217, 240, 257, 295, 461, 506, 522
CviKI_1 RGCY 9 cut(s) 77, 91, 217, 240, 257, 295, 461, 506, 522
CviQI GTAC 2 cut(s) 52, 421
DdeI CTNAG 6 cut(s) 87, 92, 118, 275, 318, 330
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
DriI GACNNNNNGTC 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 451, 493
Eam1105I GACNNNNNGTC 1 cut(s) 19
EarI CTCTTC 2 cut(s) 451, 493
Ecl136II GAGCTC 2 cut(s) 77, 91
Eco24I GRGCYC 2 cut(s) 79, 93
Eco53kI GAGCTC 2 cut(s) 77, 91
EcoICRI GAGCTC 2 cut(s) 77, 91
EcoRII CCWGG 2 cut(s) 261, 338
EcoT38I GRGCYC 2 cut(s) 79, 93
FaeI CATG 1 cut(s) 58
FaiI YATR 6 cut(s) 56, 72, 102, 197, 209, 305
FaqI GGGAC 1 cut(s) 497
FatI CATG 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 504
FriOI GRGCYC 2 cut(s) 79, 93
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 1 cut(s) 494
GlaI GCGC 1 cut(s) 8
GluI GCNGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 284
HhaI GCGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 4 cut(s) 308, 371, 412, 485
Hpy188I TCNGA 2 cut(s) 95, 333
Hpy188III TCNNGA 2 cut(s) 120, 400
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 2 cut(s) 182, 223
HpyCH4V TGCA 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 503
HpyF3I CTNAG 6 cut(s) 87, 92, 118, 275, 318, 330
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 451
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 8 cut(s) 99, 105, 134, 248, 275, 325, 352, 438
Lsp1109I GCAGC 1 cut(s) 515
MaeI CTAG 1 cut(s) 494
MaeIII GTNAC 2 cut(s) 182, 405
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 6 cut(s) 119, 166, 317, 458, 468, 480
MhlI GDGCHC 2 cut(s) 79, 93
MluCI AATT 4 cut(s) 58, 357, 377, 478
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 3 cut(s) 36, 309, 339
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 3 cut(s) 213, 360, 441
MspR9I CCNGG 2 cut(s) 263, 340
MvaI CCWGG 2 cut(s) 263, 340
MwoI GCNNNNNNNGC 2 cut(s) 88, 503
NdeII GATC 1 cut(s) 226
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 2 cut(s) 83, 345
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 182, 405
NspV TTCGAA 1 cut(s) 415
PasI CCCWGGG 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 3 cut(s) 308, 371, 412
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
Psp124BI GAGCTC 2 cut(s) 79, 93
Psp6I CCWGG 2 cut(s) 261, 338
PspGI CCWGG 2 cut(s) 261, 338
PspN4I GGNNCC 2 cut(s) 83, 345
RsaI GTAC 2 cut(s) 53, 422
RsaNI GTAC 2 cut(s) 52, 421
SacI GAGCTC 2 cut(s) 79, 93
SapI GCTCTTC 1 cut(s) 451
SaqAI TTAA 3 cut(s) 213, 360, 441
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 1 cut(s) 226
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 263, 340
SduI GDGCHC 2 cut(s) 79, 93
SfuI TTCGAA 1 cut(s) 415
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 4 cut(s) 58, 357, 377, 478
SsiI CCGC 2 cut(s) 312, 497
SspMI CTAG 1 cut(s) 494
SstI GAGCTC 2 cut(s) 79, 93
StyD4I CCNGG 2 cut(s) 261, 338
TaaI ACNGT 2 cut(s) 182, 223
TaqI TCGA 2 cut(s) 177, 415
TasI AATT 4 cut(s) 58, 357, 377, 478
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 3 cut(s) 308, 371, 412
Tru1I TTAA 3 cut(s) 213, 360, 441
Tru9I TTAA 3 cut(s) 213, 360, 441
TscAI CASTG 3 cut(s) 187, 226, 414
TseFI GTSAC 2 cut(s) 182, 405
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 2 cut(s) 182, 405
TspDTI ATGAA 4 cut(s) 59, 71, 355, 458
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 3 cut(s) 187, 226, 414
XapI RAATTY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.