Rh5BG182300

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003409
Physical Location & Seq
Forward (+)
19994666 .. 19998408
3743 bp
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UTR
Exon/CDS
Intron
Rh5BG182300.1

Sequence Viewer

Length: 615 bp
ATGGAGCGCAAAGACAATCAGTCTCCTCAAGATGAAACCGCTCGGATTCTGGTCGAGTTCTTGGAGGTGGCAATCACTTCAATCGTTTTCCTCAAAGGAATTTACCCTCCAGGTGCTTTTGAGAGAAGGAAGTATATGAATTTGGTGGTTCATAGAGCCCGGCACCCTGAGCTCAGAGATTATATCCATTCTGCTGTCTCAGGACTTCTTCCTTTTATAAAAAAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTCGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACGTCAAAGAAGCTGACCTGGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAATTGCAGGTGGGAGATAACAGCATACTTCCGGTCCCTTCCTCAGGAAATTTCAAGCAAGAATGAAGGGTTGTGGCTTCCAACAGACACAAAGCAGTGGCAACAACCTCCCCTAATAACTCCTATTAAGTCGATGAGTAGCCAACCTCTCTCTGTGCAGTTGTATCTAGAACATCCAAGTGTATATGAACCAAAGCCGTGA

Protein Analysis

204

Amino Acids

23.58

Weight (kDa)

7.76

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HORMA PF02301 18 - 151 1.3e-09 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 218
AarI CACCTGC 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 2 cut(s) 39, 393
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 3 cut(s) 99, 139, 462
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 2 cut(s) 81, 468
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 2 cut(s) 109, 342
AluBI AGCT 4 cut(s) 172, 298, 338, 376
AluI AGCT 4 cut(s) 172, 298, 338, 376
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 27, 202
AlwI GGATC 1 cut(s) 315
ApoI RAATTY 3 cut(s) 99, 139, 462
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 447
AsuC2I CCSGG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 447
AxyI CCTNAGG 1 cut(s) 456
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 2 cut(s) 160, 174
Bbv12I GWGCWC 1 cut(s) 174
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 2 cut(s) 111, 344
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 27, 202
BfaI CTAG 1 cut(s) 581
BfuAI ACCTGC 1 cut(s) 411
Bme1390I CCNGG 3 cut(s) 111, 160, 344
Bme18I GGWCC 1 cut(s) 447
BmeRI GACNNNNNGTC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 164, 449
BmrFI CCNGG 3 cut(s) 111, 160, 344
BpmI CTGGAG 2 cut(s) 93, 365
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse21I CCTNAGG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 111, 344
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 5 cut(s) 159, 187, 213, 425, 470
BseRI GAGGAG 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 2 cut(s) 160, 445
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 27, 202
BsmFI GGGAC 1 cut(s) 433
Bsp1286I GDGCHC 2 cut(s) 160, 174
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 39, 393
BspCNI CTCAG 5 cut(s) 160, 186, 212, 424, 469
BspLI GGNNCC 2 cut(s) 164, 449
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 41
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 111, 344
Bst4CI ACNGT 2 cut(s) 263, 304
BstDEI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 2 cut(s) 27, 202
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 2 cut(s) 111, 344
BstSCI CCNGG 3 cut(s) 109, 158, 342
Bsu36I CCTNAGG 1 cut(s) 456
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 3 cut(s) 268, 300, 515
BveI ACCTGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 447
CviJI RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
CviKI_1 RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
DdeI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 2 cut(s) 160, 174
Eco47I GGWCC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 109, 342
EcoT38I GRGCYC 2 cut(s) 160, 174
FaqI GGGAC 1 cut(s) 433
FokI GGATG 1 cut(s) 573
FriOI GRGCYC 2 cut(s) 160, 174
FspBI CTAG 1 cut(s) 581
GlaI GCGC 1 cut(s) 8
GsuI CTGGAG 2 cut(s) 93, 365
HapII CCGG 2 cut(s) 160, 445
HhaI GCGC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 46, 389
HpaII CCGG 2 cut(s) 160, 445
Hpy188I TCNGA 3 cut(s) 45, 176, 414
Hpy188III TCNNGA 4 cut(s) 29, 201, 458, 581
HpyAV CCTTC 3 cut(s) 120, 461, 473
HpyCH4III ACNGT 2 cut(s) 263, 304
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 420, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
HpySE526I ACGT 1 cut(s) 327
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 4
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 263
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 200, 247, 398
MhlI GDGCHC 2 cut(s) 160, 174
MluCI AATT 4 cut(s) 99, 139, 415, 462
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 9 cut(s) 36, 58, 101, 117, 390, 420, 465, 531, 570
MseI TTAA 2 cut(s) 294, 540
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 2 cut(s) 160, 445
MspR9I CCNGG 3 cut(s) 111, 160, 344
MvaI CCWGG 2 cut(s) 111, 344
MwoI GCNNNNNNNGC 1 cut(s) 169
NciI CCSGG 1 cut(s) 160
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 164, 449
NmuCI GTSAC 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 46, 389
PsiI TTATAA 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 109, 342
PspGI CCWGG 2 cut(s) 109, 342
PspN4I GGNNCC 2 cut(s) 164, 449
PspPI GGNCC 1 cut(s) 447
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 2 cut(s) 294, 540
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 447
ScrFI CCNGG 3 cut(s) 111, 160, 344
SduI GDGCHC 2 cut(s) 160, 174
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 4 cut(s) 99, 139, 415, 462
SsiI CCGC 2 cut(s) 39, 393
SspMI CTAG 1 cut(s) 581
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 109, 158, 342
TaaI ACNGT 2 cut(s) 263, 304
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 54, 258, 545
TasI AATT 4 cut(s) 99, 139, 415, 462
TfiI GAWTC 2 cut(s) 46, 389
Tru1I TTAA 2 cut(s) 294, 540
Tru9I TTAA 2 cut(s) 294, 540
TscAI CASTG 3 cut(s) 268, 307, 515
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 48, 140, 152, 492, 615
TspRI CASTG 3 cut(s) 268, 307, 515
VpaK11BI GGWCC 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 3 cut(s) 99, 139, 462
XbaI TCTAGA 1 cut(s) 580
XspI CTAG 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.