Rh4CG215700

Vacuole membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003409
Physical Location & Seq
Reverse (-)
44625731 .. 44627875
2145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG215700.1

Sequence Viewer

Length: 324 bp
ATGGACGGCGTAGCTGGCCTAGGCTACTTCAATTTGGTCAAGGAGGAGATCGACAAGGCTTTTAAGGCTGAAGAATGGGGCTTAAGATCGGCGTCGACAACACAGTTAAGATTAGTTTCTGTACTTCTTGCTCTTACAAGTCCTTCATGGATGGATAGAAACTACTTTGAATATGGTCCCCCACTATTGTCATCCCAGCAAGGTTCAAGGCTCCCACTTAGCAGCATACTGCCTCAGGTTCAGTTAGAGGCTATACTATGGGGAATTGGAACTGCACTGGGAGAGCTTCCTCCATTTTTCATATCAAGAGCTGGTAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 map04140
Pfam Domains
Protein Families
No domains found.

Protein Analysis

107

Amino Acids

11.79

Weight (kDa)

4.96

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 95
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 123
AflII CTTAAG 1 cut(s) 82
AgsI TTSAA 3 cut(s) 31, 170, 207
AluBI AGCT 3 cut(s) 14, 286, 311
AluI AGCT 3 cut(s) 14, 286, 311
AoxI GGCC 1 cut(s) 16
ApeKI GCWGC 1 cut(s) 222
AspA2I CCTAGG 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 176
AvaII GGWCC 1 cut(s) 176
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 234
BbvI GCAGC 1 cut(s) 234
BccI CCATC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 22
BfaI CTAG 1 cut(s) 20
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 1 cut(s) 223
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 178, 212
BmrI ACTGGG 1 cut(s) 287
BmuI ACTGGG 1 cut(s) 287
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 282
Bse21I CCTNAGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 2 cut(s) 156, 191
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 234
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 1 cut(s) 18
BslFI GGGAC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 48, 86
BspANI GGCC 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 178, 212
BspTI CTTAAG 1 cut(s) 82
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 2 cut(s) 48, 86
BssNI GRCGYC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 92
BstAFI CTTAAG 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 2 cut(s) 218, 234
BstF5I GGATG 2 cut(s) 156, 191
BstKTI GATC 2 cut(s) 51, 89
BstMBI GATC 2 cut(s) 48, 86
BstMWI GCNNNNNNNGC 2 cut(s) 15, 65
BstV1I GCAGC 1 cut(s) 234
Bsu36I CCTNAGG 1 cut(s) 234
BsuRI GGCC 1 cut(s) 18
BtsCI GGATG 2 cut(s) 156, 191
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 81
Csp6I GTAC 1 cut(s) 122
CviAII CATG 1 cut(s) 147
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 2 cut(s) 218, 234
DpnI GATC 2 cut(s) 50, 88
DpnII GATC 2 cut(s) 48, 86
Eco130I CCWWGG 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 1 cut(s) 150
FaiI YATR 6 cut(s) 148, 174, 227, 254, 259, 302
FaqI GGGAC 1 cut(s) 162
FatI CATG 1 cut(s) 146
FblI GTMKAC 1 cut(s) 95
Fnu4HI GCNGC 1 cut(s) 223
FokI GGATG 2 cut(s) 163, 178
Fsp4HI GCNGC 1 cut(s) 223
FspBI CTAG 1 cut(s) 20
GluI GCNGC 1 cut(s) 223
GsaI CCCAGC 1 cut(s) 199
HaeIII GGCC 1 cut(s) 18
HgaI GACGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 1 cut(s) 150
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
Hpy166II GTNNAC 1 cut(s) 96
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 1 cut(s) 96
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4V TGCA 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 65
HpyF3I CTNAG 2 cut(s) 218, 234
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 1 cut(s) 150
Kzo9I GATC 2 cut(s) 48, 86
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 4 cut(s) 209, 221, 263, 297
Lsp1109I GCAGC 1 cut(s) 234
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 314
MalI GATC 2 cut(s) 50, 88
MboI GATC 2 cut(s) 48, 86
MboII GAAGA 1 cut(s) 83
MluCI AATT 2 cut(s) 31, 264
MnlI CCTC 4 cut(s) 37, 241, 243, 300
MseI TTAA 3 cut(s) 63, 83, 107
MspCI CTTAAG 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 15, 65
NdeII GATC 2 cut(s) 48, 86
NlaIII CATG 1 cut(s) 150
NlaIV GGNNCC 2 cut(s) 178, 212
PkrI GCNGC 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 178, 212
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SalI GTCGAC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 63, 83, 107
SatI GCNGC 1 cut(s) 223
Sau3AI GATC 2 cut(s) 48, 86
Sau96I GGNCC 1 cut(s) 176
SetI ASST 5 cut(s) 16, 205, 240, 288, 313
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 2 cut(s) 31, 264
SspMI CTAG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 51, 95
TasI AATT 2 cut(s) 31, 264
TatI WGTACW 1 cut(s) 121
Tru1I TTAA 3 cut(s) 63, 83, 107
Tru9I TTAA 3 cut(s) 63, 83, 107
TscAI CASTG 1 cut(s) 282
TseI GCWGC 1 cut(s) 222
TspDTI ATGAA 2 cut(s) 135, 289
TspRI CASTG 1 cut(s) 282
Vha464I CTTAAG 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 95
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.