Rw5G016820

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003409
Physical Location & Seq
Forward (+)
21455164 .. 21458071
2908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016820.1

Sequence Viewer

Length: 435 bp
ATGGAGCGCAAAGACAATCAGTCTCCTCAAGATGAAACCGCTCGGATTCTGGTCGAGTTCTTGGAGGTGGCAATCACTTCAATCGTTTTCCTCAAAGGAATTTACCCCTCAGGTGCTTTTGAGAGAAGGAAGTATATGAATTTGGTGGTTCATAGAGCTCGGCACCCTGAGCTCAGAGATTATATCCATTCTGCTGTCTCAGGACTTCTTCCTTTTATCAAAAAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTCGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACGTCAAAGAAGCTGACCTGGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAGTATGTCCTTAAGTTCATAG

Protein Analysis

144

Amino Acids

16.47

Weight (kDa)

7.88

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HORMA PF02301 18 - 108 2.3e-09 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 2 cut(s) 39, 393
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 2 cut(s) 99, 139
AflII CTTAAG 1 cut(s) 424
AgsI TTSAA 1 cut(s) 81
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 5 cut(s) 158, 172, 298, 338, 376
AluI AGCT 5 cut(s) 158, 172, 298, 338, 376
Alw21I GWGCWC 2 cut(s) 160, 174
Alw26I GTCTC 2 cut(s) 27, 202
AlwI GGATC 1 cut(s) 315
ApoI RAATTY 2 cut(s) 99, 139
AspLEI GCGC 1 cut(s) 9
AxyI CCTNAGG 1 cut(s) 109
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 2 cut(s) 160, 174
Bbv12I GWGCWC 2 cut(s) 160, 174
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 27, 202
BfrI CTTAAG 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 344
BmeRI GACNNNNNGTC 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 344
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 12
Bse21I CCTNAGG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 344
BseMII CTCAG 5 cut(s) 123, 159, 187, 213, 425
BseRI GAGGAG 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 160, 174
BsmAI GTCTC 2 cut(s) 27, 202
Bsp1286I GDGCHC 2 cut(s) 160, 174
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 39, 393
BspCNI CTCAG 5 cut(s) 122, 160, 186, 212, 424
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 41
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 263, 304
BstAFI CTTAAG 1 cut(s) 424
BstDEI CTNAG 7 cut(s) 109, 168, 173, 199, 356, 399, 411
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 2 cut(s) 27, 202
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 342
Bsu36I CCTNAGG 1 cut(s) 109
BtsIMutI CAGTG 2 cut(s) 268, 300
CfoI GCGC 1 cut(s) 9
CviJI RGCY 6 cut(s) 158, 172, 298, 321, 338, 376
CviKI_1 RGCY 6 cut(s) 158, 172, 298, 321, 338, 376
DdeI CTNAG 7 cut(s) 109, 168, 173, 199, 356, 399, 411
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 2 cut(s) 158, 172
Eco24I GRGCYC 2 cut(s) 160, 174
Eco53kI GAGCTC 2 cut(s) 158, 172
Eco81I CCTNAGG 1 cut(s) 109
EcoICRI GAGCTC 2 cut(s) 158, 172
EcoRII CCWGG 1 cut(s) 342
EcoT38I GRGCYC 2 cut(s) 160, 174
FaiI YATR 9 cut(s) 135, 137, 153, 183, 278, 290, 386, 419, 433
FriOI GRGCYC 2 cut(s) 160, 174
GlaI GCGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 365
HhaI GCGC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 46, 389
Hpy188I TCNGA 3 cut(s) 45, 176, 414
Hpy188III TCNNGA 2 cut(s) 29, 201
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 2 cut(s) 263, 304
HpyCH4IV ACGT 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 7 cut(s) 109, 168, 173, 199, 356, 399, 411
HpySE526I ACGT 1 cut(s) 327
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 35, 96, 180, 186, 215, 329, 356
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 263
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 200, 247, 398
MhlI GDGCHC 2 cut(s) 160, 174
MluCI AATT 2 cut(s) 99, 139
MnlI CCTC 6 cut(s) 36, 58, 101, 118, 390, 420
MseI TTAA 2 cut(s) 294, 425
MspCI CTTAAG 1 cut(s) 424
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 1 cut(s) 169
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 1 cut(s) 164
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 46, 389
Psp124BI GAGCTC 2 cut(s) 160, 174
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 164
SacI GAGCTC 2 cut(s) 160, 174
SaqAI TTAA 2 cut(s) 294, 425
Sau3AI GATC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 2 cut(s) 160, 174
SmlI CTYRAG 2 cut(s) 27, 424
SmoI CTYRAG 2 cut(s) 27, 424
Sse9I AATT 2 cut(s) 99, 139
SsiI CCGC 2 cut(s) 39, 393
SstI GAGCTC 2 cut(s) 160, 174
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 263, 304
TaiI ACGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 54, 258
TasI AATT 2 cut(s) 99, 139
TfiI GAWTC 2 cut(s) 46, 389
Tru1I TTAA 2 cut(s) 294, 425
Tru9I TTAA 2 cut(s) 294, 425
TscAI CASTG 2 cut(s) 268, 307
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 4 cut(s) 48, 140, 152, 420
TspRI CASTG 2 cut(s) 268, 307
Vha464I CTTAAG 1 cut(s) 424
XapI RAATTY 2 cut(s) 99, 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.