MD10G1306000.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001958
Physical Location & Seq
Forward (+)
39194871 .. 39197817
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1306000.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCTTCTCCCCGAAAGAGCCCGTTATCAGAACCCGGAGATCGCCAGTCCGCCGCTGCAATCGTAGTCCAACCGTCGTCCCCTCGCTACCCTCCGAGCGGGACGCCCACCTCCGGAGCCCAGCGCCGAATCGGGATCGCCGTCGATCTCAGCGACGAGAGCGCCTTCGCCGTCAAATGGGCCGTCCAGAACTACCTCCGCCCTGGCGATGCTGTCATCCTCCTCCACGTCCGCCCCACTAGCGTCCTCTACGGCGCCGACTGGGGCGCCACCGACGTCTCCATCCCCGAAGACCAGGAGTCCCAGCAGAAGCTCGAGGACGATTTCGATAATTTCACCGTCACCAAGGCCAACGACCTCGCCAGGCCGCTCGAGGAGGCCAGGACGCCGTACAAGATCCACATAGTCAAGGACCACGACATGAAGGAGCGGTTGTGCTTGGAGGTCGAGAGGCTCGGCCTCAGCGCCGTCGTGATGGGCAGCCGGGGATTCGGGGCTTCGAAGCGGACCTCCAAGGCCAGGCTCGGCAGTGTCAGTGACTACTGTGTGCACCACTGCGTCTGTCCTGTTATCGTCGTGAGGTTCTCCGATGACAAGGATGATGAAAACGACGCTGAAAAGCAAGGTAAATTGGACTGCCCCGTCGTGCCTGAGGAGGAGCCGGAGTTTCATGACGCTTTGGATAAAGACGCAGATAAGGCGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

236

Amino Acids

25.83

Weight (kDa)

5.12

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 42 - 194 7.7e-28 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 2 cut(s) 254, 266
AccBSI CCGCTC 3 cut(s) 99, 370, 430
AccII CGCG 1 cut(s) 703
AccIII TCCGGA 1 cut(s) 113
AciI CCGC 8 cut(s) 51, 54, 99, 199, 232, 368, 430, 505
AclWI GGATC 2 cut(s) 143, 391
AcyI GRCGYC 5 cut(s) 104, 255, 267, 276, 386
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 4 cut(s) 98, 113, 177, 519
AhdI GACNNNNNGTC 1 cut(s) 298
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 5 cut(s) 202, 294, 362, 380, 518
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 283
Alw44I GTGCAC 1 cut(s) 548
AlwI GGATC 2 cut(s) 143, 391
Ama87I CYCGRG 2 cut(s) 314, 371
Aor13HI TCCGGA 1 cut(s) 113
AoxI GGCC 6 cut(s) 180, 348, 365, 378, 457, 516
ApaLI GTGCAC 1 cut(s) 548
ApeKI GCWGC 2 cut(s) 56, 480
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AspLEI GCGC 7 cut(s) 126, 164, 257, 269, 467, 703, 705
AspS9I GGNCC 3 cut(s) 180, 412, 507
AsuC2I CCSGG 2 cut(s) 36, 485
AsuHPI GGTGA 2 cut(s) 328, 334
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 2 cut(s) 314, 371
AvaII GGWCC 2 cut(s) 412, 507
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 552
BanI GGYRCC 2 cut(s) 254, 266
BanII GRGCYC 2 cut(s) 23, 121
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 552
BbvCI CCTCAGC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 43, 492
BccI CCATC 2 cut(s) 290, 469
BceAI ACGGC 6 cut(s) 125, 155, 167, 268, 373, 452
BciT130I CCWGG 5 cut(s) 204, 296, 364, 382, 520
BcnI CCSGG 2 cut(s) 36, 485
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 1 cut(s) 240
BfoI RGCGCY 5 cut(s) 127, 165, 258, 270, 468
BisI GCNGC 4 cut(s) 54, 57, 368, 481
BlsI GCNGC 4 cut(s) 55, 58, 369, 482
Bme1390I CCNGG 7 cut(s) 36, 204, 296, 364, 382, 485, 520
Bme18I GGWCC 2 cut(s) 412, 507
BmeRI GACNNNNNGTC 1 cut(s) 298
BmeT110I CYCGRG 2 cut(s) 314, 371
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 180, 412, 507
BmiI GGNNCC 4 cut(s) 118, 256, 268, 660
BmrFI CCNGG 7 cut(s) 36, 204, 296, 364, 382, 485, 520
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 199
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 36, 485
BsaHI GRCGYC 5 cut(s) 104, 255, 267, 276, 386
BsaJI CCNNGG 4 cut(s) 202, 345, 484, 513
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 4 cut(s) 419, 449, 650, 680
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 113, 177, 519
Bse1I ACTGG 2 cut(s) 46, 266
Bse21I CCTNAGG 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 113
BseBI CCWGG 5 cut(s) 204, 296, 364, 382, 520
BseDI CCNNGG 4 cut(s) 202, 345, 484, 513
BseGI GGATG 3 cut(s) 216, 282, 604
BseLI CCNNNNNNNGG 4 cut(s) 98, 113, 177, 519
BseMII CTCAG 3 cut(s) 163, 475, 642
BseNI ACTGG 2 cut(s) 46, 266
BsePI GCGCGC 1 cut(s) 701
BseRI GAGGAG 4 cut(s) 212, 389, 668, 671
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 2 cut(s) 43, 492
BseYI CCCAGC 2 cut(s) 120, 303
Bsh1236I CGCG 1 cut(s) 703
BshFI GGCC 6 cut(s) 182, 350, 367, 380, 459, 518
BshNI GGYRCC 2 cut(s) 254, 266
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 2 cut(s) 314, 371
BsiSI CCGG 4 cut(s) 36, 114, 484, 662
BslFI GGGAC 3 cut(s) 64, 115, 286
BslI CCNNNNNNNGG 4 cut(s) 98, 113, 177, 519
BsmAI GTCTC 1 cut(s) 283
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 3 cut(s) 64, 115, 286
BsnI GGCC 6 cut(s) 182, 350, 367, 380, 459, 518
BsoBI CYCGRG 2 cut(s) 314, 371
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 3 cut(s) 23, 121, 552
Bsp13I TCCGGA 1 cut(s) 113
Bsp143I GATC 4 cut(s) 40, 135, 145, 396
BspACI CCGC 8 cut(s) 51, 54, 99, 199, 232, 368, 430, 505
BspANI GGCC 6 cut(s) 182, 350, 367, 380, 459, 518
BspCNI CTCAG 3 cut(s) 162, 474, 643
BspEI TCCGGA 1 cut(s) 113
BspFNI CGCG 1 cut(s) 703
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 4 cut(s) 118, 256, 268, 660
BspPI GGATC 2 cut(s) 143, 391
BspT104I TTCGAA 1 cut(s) 500
BspT107I GGYRCC 2 cut(s) 254, 266
BsrBI CCGCTC 3 cut(s) 99, 370, 430
BsrI ACTGG 2 cut(s) 46, 266
BssECI CCNNGG 4 cut(s) 202, 345, 484, 513
BssHII GCGCGC 1 cut(s) 701
BssMI GATC 4 cut(s) 40, 135, 145, 396
BssNI GRCGYC 5 cut(s) 104, 255, 267, 276, 386
BssT1I CCWWGG 2 cut(s) 345, 513
Bst2UI CCWGG 5 cut(s) 204, 296, 364, 382, 520
Bst4CI ACNGT 3 cut(s) 75, 340, 545
BstACI GRCGYC 5 cut(s) 104, 255, 267, 276, 386
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 703
BstDEI CTNAG 3 cut(s) 149, 461, 651
BstF5I GGATG 3 cut(s) 216, 282, 604
BstFNI CGCG 1 cut(s) 703
BstH2I RGCGCY 5 cut(s) 127, 165, 258, 270, 468
BstHHI GCGC 7 cut(s) 126, 164, 257, 269, 467, 703, 705
BstKTI GATC 4 cut(s) 43, 138, 148, 399
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 4 cut(s) 40, 135, 145, 396
BstMWI GCNNNNNNNGC 3 cut(s) 159, 240, 698
BstNI CCWGG 5 cut(s) 204, 296, 364, 382, 520
BstSCI CCNGG 7 cut(s) 34, 202, 294, 362, 380, 483, 518
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 43, 492
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 6 cut(s) 182, 350, 367, 380, 459, 518
BtgZI GCGATG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 3 cut(s) 216, 282, 604
BtsI GCAGTG 2 cut(s) 535, 553
BtsIMutI CAGTG 3 cut(s) 535, 541, 553
Cac8I GCNNGC 1 cut(s) 703
CciI TCATGA 1 cut(s) 670
CfoI GCGC 7 cut(s) 126, 164, 257, 269, 467, 703, 705
Cfr13I GGNCC 3 cut(s) 180, 412, 507
CseI GACGC 7 cut(s) 112, 232, 394, 547, 620, 683, 698
Csp6I GTAC 1 cut(s) 391
CviAII CATG 2 cut(s) 421, 671
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 149, 461, 651
DinI GGCGCC 2 cut(s) 256, 268
DpnI GATC 4 cut(s) 42, 137, 147, 398
DpnII GATC 4 cut(s) 40, 135, 145, 396
DrdI GACNNNNNNGTC 1 cut(s) 641
DriI GACNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 1 cut(s) 641
Eam1105I GACNNNNNGTC 1 cut(s) 298
EciI GGCGGA 3 cut(s) 40, 188, 221
Eco130I CCWWGG 2 cut(s) 345, 513
Eco24I GRGCYC 2 cut(s) 23, 121
Eco47I GGWCC 2 cut(s) 412, 507
Eco81I CCTNAGG 1 cut(s) 651
Eco88I CYCGRG 2 cut(s) 314, 371
EcoRII CCWGG 5 cut(s) 202, 294, 362, 380, 518
EcoT14I CCWWGG 2 cut(s) 345, 513
EcoT38I GRGCYC 2 cut(s) 23, 121
EgeI GGCGCC 2 cut(s) 256, 268
EheI GGCGCC 2 cut(s) 256, 268
ErhI CCWWGG 2 cut(s) 345, 513
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 2 cut(s) 424, 674
FaiI YATR 3 cut(s) 404, 422, 672
FaqI GGGAC 3 cut(s) 64, 115, 286
FatI CATG 2 cut(s) 420, 670
FauI CCCGC 1 cut(s) 92
Fnu4HI GCNGC 4 cut(s) 54, 57, 368, 481
FokI GGATG 3 cut(s) 203, 269, 611
FriOI GRGCYC 2 cut(s) 23, 121
Fsp4HI GCNGC 4 cut(s) 54, 57, 368, 481
FspBI CTAG 1 cut(s) 240
GlaI GCGC 7 cut(s) 125, 163, 256, 268, 466, 702, 704
GluI GCNGC 4 cut(s) 54, 57, 368, 481
GsaI CCCAGC 2 cut(s) 124, 307
HaeII RGCGCY 5 cut(s) 127, 165, 258, 270, 468
HaeIII GGCC 6 cut(s) 182, 350, 367, 380, 459, 518
HapII CCGG 4 cut(s) 36, 114, 484, 662
HgaI GACGC 7 cut(s) 112, 232, 394, 547, 620, 683, 698
HhaI GCGC 7 cut(s) 126, 164, 257, 269, 467, 703, 705
Hin1I GRCGYC 5 cut(s) 104, 255, 267, 276, 386
Hin1II CATG 2 cut(s) 424, 674
Hin6I GCGC 7 cut(s) 124, 162, 255, 267, 465, 701, 703
HinP1I GCGC 7 cut(s) 124, 162, 255, 267, 465, 701, 703
HinfI GANTC 3 cut(s) 129, 299, 489
HpaII CCGG 4 cut(s) 36, 114, 484, 662
HphI GGTGA 2 cut(s) 328, 334
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 3 cut(s) 31, 96, 589
Hpy188III TCNNGA 7 cut(s) 114, 133, 187, 448, 472, 577, 671
Hpy8I GTNNAC 1 cut(s) 550
Hpy99I CGWCG 8 cut(s) 79, 146, 158, 278, 473, 578, 614, 647
HpyAV CCTTC 2 cut(s) 175, 418
HpyCH4III ACNGT 3 cut(s) 75, 340, 545
HpyCH4IV ACGT 2 cut(s) 228, 276
HpyCH4V TGCA 2 cut(s) 59, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 240, 698
HpyF3I CTNAG 3 cut(s) 149, 461, 651
HpySE526I ACGT 2 cut(s) 228, 276
Hsp92I GRCGYC 5 cut(s) 104, 255, 267, 276, 386
Hsp92II CATG 2 cut(s) 424, 674
HspAI GCGC 7 cut(s) 124, 162, 255, 267, 465, 701, 703
KasI GGCGCC 2 cut(s) 254, 266
Kpn2I TCCGGA 1 cut(s) 113
Kzo9I GATC 4 cut(s) 40, 135, 145, 396
LmnI GCTCC 3 cut(s) 116, 427, 658
Lsp1109I GCAGC 2 cut(s) 43, 492
LweI GCATC 1 cut(s) 199
MaeI CTAG 1 cut(s) 240
MaeII ACGT 2 cut(s) 228, 276
MaeIII GTNAC 2 cut(s) 340, 536
MalI GATC 4 cut(s) 42, 137, 147, 398
MbiI CCGCTC 3 cut(s) 99, 370, 430
MboI GATC 4 cut(s) 40, 135, 145, 396
MboII GAAGA 1 cut(s) 302
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 3 cut(s) 23, 121, 552
MluCI AATT 2 cut(s) 331, 629
Mly113I GGCGCC 2 cut(s) 255, 267
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 94
MroI TCCGGA 1 cut(s) 113
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 4 cut(s) 36, 114, 484, 662
MspR9I CCNGG 7 cut(s) 36, 204, 296, 364, 382, 485, 520
MvaI CCWGG 5 cut(s) 204, 296, 364, 382, 520
MvnI CGCG 1 cut(s) 703
MwoI GCNNNNNNNGC 3 cut(s) 159, 240, 698
NarI GGCGCC 2 cut(s) 255, 267
NciI CCSGG 2 cut(s) 36, 485
NdeII GATC 4 cut(s) 40, 135, 145, 396
NlaIII CATG 2 cut(s) 424, 674
NlaIV GGNNCC 4 cut(s) 118, 256, 268, 660
NmeAIII GCCGAG 2 cut(s) 435, 504
NmuCI GTSAC 2 cut(s) 340, 536
NspV TTCGAA 1 cut(s) 500
PaeR7I CTCGAG 2 cut(s) 314, 371
PagI TCATGA 1 cut(s) 670
PauI GCGCGC 1 cut(s) 701
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 150
PfeI GAWTC 2 cut(s) 129, 489
PkrI GCNGC 4 cut(s) 55, 58, 369, 482
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 2 cut(s) 258, 270
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 5 cut(s) 202, 294, 362, 380, 518
PspFI CCCAGC 2 cut(s) 120, 303
PspGI CCWGG 5 cut(s) 202, 294, 362, 380, 518
PspN4I GGNNCC 4 cut(s) 118, 256, 268, 660
PspPI GGNCC 3 cut(s) 180, 412, 507
PspXI VCTCGAGB 2 cut(s) 314, 371
PsuI RGATCY 1 cut(s) 396
PteI GCGCGC 1 cut(s) 701
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SatI GCNGC 4 cut(s) 54, 57, 368, 481
Sau3AI GATC 4 cut(s) 40, 135, 145, 396
Sau96I GGNCC 3 cut(s) 180, 412, 507
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 7 cut(s) 36, 204, 296, 364, 382, 485, 520
SduI GDGCHC 3 cut(s) 23, 121, 552
SfaNI GCATC 1 cut(s) 199
SfoI GGCGCC 2 cut(s) 256, 268
Sfr274I CTCGAG 2 cut(s) 314, 371
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 2 cut(s) 412, 507
SlaI CTCGAG 2 cut(s) 314, 371
SmlI CTYRAG 2 cut(s) 314, 371
SmoI CTYRAG 2 cut(s) 314, 371
Sse9I AATT 2 cut(s) 331, 629
SsiI CCGC 8 cut(s) 51, 54, 99, 199, 232, 368, 430, 505
SspDI GGCGCC 2 cut(s) 254, 266
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 7 cut(s) 34, 202, 294, 362, 380, 483, 518
StyI CCWWGG 2 cut(s) 345, 513
TaaI ACNGT 3 cut(s) 75, 340, 545
TaiI ACGT 2 cut(s) 231, 279
TaqI TCGA 6 cut(s) 144, 315, 327, 372, 447, 500
TasI AATT 2 cut(s) 331, 629
TauI GCSGC 2 cut(s) 56, 370
TfiI GAWTC 2 cut(s) 129, 489
TscAI CASTG 3 cut(s) 535, 541, 560
TseFI GTSAC 2 cut(s) 340, 536
TseI GCWGC 2 cut(s) 56, 480
Tsp45I GTSAC 2 cut(s) 340, 536
TspDTI ATGAA 3 cut(s) 437, 618, 659
TspRI CASTG 3 cut(s) 535, 541, 560
VneI GTGCAC 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 412, 507
XhoI CTCGAG 2 cut(s) 314, 371
XspI CTAG 1 cut(s) 240
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.