pycom05g30240

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001958
Physical Location & Seq
Forward (+)
30403615 .. 30406265
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30240.1

Sequence Viewer

Length: 708 bp
ATGGCTTCTCCCAGAAAGAGCCCGTTATCGGAACCCGGAGACCGCCAGTCCGCTGCGGCAATCGTAGTCCAACCGTCGTCCCCGCGCTACCCTCCCAGCGGGACCCCCACCTCCGGAGCCCAGCGACGGATCGGGATCGCCGTCGATCTCAGCGACGAGAGCGCCTTCGCTGTTAAATGGGCCGTCCAGAACTACCTCCGCCCCGGAGACGCCGTCATCCTCCTCCACGTCCGTCCCACCAGCGTCCTCTACGGCGCCGACTGGGGCGCCACCGACGTCTCCATCCCCGAGGACCAGGAGTCCCAGCAGAAGCTCGAGGACGATTTCGACAATTTCACCGTCACCAAGGCCAACGACCTCGCCAAGCCGCTCGAGGAGGCCAAGACGCCATACAAGATCCACATAGTCAAGGACCACGACATGAAGGAGCGGTTGTGTTTGGAGGTTGAGAGGCTTGGCCTCAGCGCCGTCGTGATGGGCAGCCGGGGATTCGGGGCTTCTAAGCGGACCTCCAAGGCCAGGCTCGGCAGTGTCAGTGACTACTGCGTGCAACACTGCGTTTGCCCTGTCATCGTCGTGAGGTTCTCCGATGACAAGGATGATGAAAACGACGCCGTAAAGCAAGGTAACTTTGACTGCCCCGTCGTGCCTGAGGAGGAGCCGGAGTTTCATGACGCTTTGCATAAAGATGCCGATAAGGCGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

236

Amino Acids

25.76

Weight (kDa)

5.18

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 42 - 194 7.5e-28 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 2 cut(s) 254, 266
AccBSI CCGCTC 2 cut(s) 370, 430
AccII CGCG 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 113
AciI CCGC 9 cut(s) 43, 51, 56, 83, 99, 199, 368, 430, 505
AclWI GGATC 3 cut(s) 137, 143, 391
AcyI GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
AfiI CCNNNNNNNGG 5 cut(s) 28, 98, 113, 126, 519
AhdI GACNNNNNGTC 2 cut(s) 46, 298
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 294, 518
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw26I GTCTC 3 cut(s) 33, 201, 283
AlwI GGATC 3 cut(s) 137, 143, 391
Ama87I CYCGRG 3 cut(s) 287, 314, 371
Aor13HI TCCGGA 1 cut(s) 113
AoxI GGCC 5 cut(s) 180, 348, 378, 457, 516
ApeKI GCWGC 2 cut(s) 53, 480
AspLEI GCGC 6 cut(s) 87, 164, 257, 269, 467, 703
AspS9I GGNCC 5 cut(s) 102, 180, 292, 412, 507
AsuC2I CCSGG 3 cut(s) 36, 204, 485
AsuHPI GGTGA 2 cut(s) 328, 334
AvaI CYCGRG 3 cut(s) 287, 314, 371
AvaII GGWCC 4 cut(s) 102, 292, 412, 507
AxyI CCTNAGG 1 cut(s) 651
BanI GGYRCC 2 cut(s) 254, 266
BanII GRGCYC 2 cut(s) 23, 121
BbvCI CCTCAGC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 40, 492
BccI CCATC 2 cut(s) 290, 469
BceAI ACGGC 6 cut(s) 125, 167, 197, 268, 452, 599
BciT130I CCWGG 2 cut(s) 296, 520
BcnI CCSGG 3 cut(s) 36, 204, 485
BcoDI GTCTC 3 cut(s) 33, 201, 283
BfoI RGCGCY 4 cut(s) 165, 258, 270, 468
BglI GCCNNNNNGGC 1 cut(s) 698
BisI GCNGC 4 cut(s) 54, 57, 368, 481
BlsI GCNGC 4 cut(s) 55, 58, 369, 482
Bme1390I CCNGG 5 cut(s) 36, 204, 296, 485, 520
Bme18I GGWCC 4 cut(s) 102, 292, 412, 507
BmeRI GACNNNNNGTC 2 cut(s) 46, 298
BmeT110I CYCGRG 3 cut(s) 287, 314, 371
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 5 cut(s) 102, 180, 292, 412, 507
BmiI GGNNCC 7 cut(s) 33, 103, 104, 118, 256, 268, 660
BmrFI CCNGG 5 cut(s) 36, 204, 296, 485, 520
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 679
BmuI ACTGGG 1 cut(s) 271
Bpu10I CCTNAGC 1 cut(s) 461
BpuMI CCSGG 3 cut(s) 36, 204, 485
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 5 cut(s) 202, 288, 345, 484, 513
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 419, 449, 650, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 98, 113, 126, 519
Bse1I ACTGG 2 cut(s) 46, 266
Bse21I CCTNAGG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 1 cut(s) 113
BseBI CCWGG 2 cut(s) 296, 520
BseDI CCNNGG 5 cut(s) 202, 288, 345, 484, 513
BseGI GGATG 3 cut(s) 216, 282, 604
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 5 cut(s) 28, 98, 113, 126, 519
BseMII CTCAG 3 cut(s) 163, 475, 642
BseNI ACTGG 2 cut(s) 46, 266
BseRI GAGGAG 4 cut(s) 212, 389, 668, 671
BseXI GCAGC 2 cut(s) 40, 492
BseYI CCCAGC 3 cut(s) 95, 120, 303
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 5 cut(s) 182, 350, 380, 459, 518
BshNI GGYRCC 2 cut(s) 254, 266
BsiHKCI CYCGRG 3 cut(s) 287, 314, 371
BsiSI CCGG 5 cut(s) 36, 114, 204, 484, 662
BslFI GGGAC 4 cut(s) 64, 115, 219, 286
BslI CCNNNNNNNGG 5 cut(s) 28, 98, 113, 126, 519
BsmAI GTCTC 3 cut(s) 33, 201, 283
BsmBI CGTCTC 2 cut(s) 201, 283
BsmFI GGGAC 4 cut(s) 64, 115, 219, 286
BsnI GGCC 5 cut(s) 182, 350, 380, 459, 518
Bso31I GGTCTC 1 cut(s) 33
BsoBI CYCGRG 3 cut(s) 287, 314, 371
Bsp1286I GDGCHC 2 cut(s) 23, 121
Bsp13I TCCGGA 1 cut(s) 113
Bsp143I GATC 4 cut(s) 129, 135, 145, 396
BspACI CCGC 9 cut(s) 43, 51, 56, 83, 99, 199, 368, 430, 505
BspANI GGCC 5 cut(s) 182, 350, 380, 459, 518
BspCNI CTCAG 3 cut(s) 162, 474, 643
BspEI TCCGGA 1 cut(s) 113
BspFNI CGCG 1 cut(s) 85
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 7 cut(s) 33, 103, 104, 118, 256, 268, 660
BspPI GGATC 3 cut(s) 137, 143, 391
BspT107I GGYRCC 2 cut(s) 254, 266
BspTNI GGTCTC 1 cut(s) 33
BsrBI CCGCTC 2 cut(s) 370, 430
BsrI ACTGG 2 cut(s) 46, 266
BssECI CCNNGG 5 cut(s) 202, 288, 345, 484, 513
BssMI GATC 4 cut(s) 129, 135, 145, 396
BssNI GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
BssT1I CCWWGG 2 cut(s) 345, 513
Bst2UI CCWGG 2 cut(s) 296, 520
Bst4CI ACNGT 2 cut(s) 75, 340
BstACI GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 4 cut(s) 149, 461, 501, 651
BstF5I GGATG 3 cut(s) 216, 282, 604
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 4 cut(s) 165, 258, 270, 468
BstHHI GCGC 6 cut(s) 87, 164, 257, 269, 467, 703
BstKTI GATC 4 cut(s) 132, 138, 148, 399
BstMAI GTCTC 3 cut(s) 33, 201, 283
BstMBI GATC 4 cut(s) 129, 135, 145, 396
BstMWI GCNNNNNNNGC 2 cut(s) 159, 698
BstNI CCWGG 2 cut(s) 296, 520
BstSCI CCNGG 5 cut(s) 34, 202, 294, 483, 518
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 40, 492
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 5 cut(s) 182, 350, 380, 459, 518
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 3 cut(s) 216, 282, 604
BtsI GCAGTG 2 cut(s) 535, 553
BtsIMutI CAGTG 4 cut(s) 535, 541, 553, 703
Cac8I GCNNGC 1 cut(s) 548
CciI TCATGA 1 cut(s) 670
CfoI GCGC 6 cut(s) 87, 164, 257, 269, 467, 703
Cfr13I GGNCC 5 cut(s) 102, 180, 292, 412, 507
CseI GACGC 5 cut(s) 218, 232, 394, 620, 683
CviAII CATG 2 cut(s) 421, 671
DdeI CTNAG 4 cut(s) 149, 461, 501, 651
DinI GGCGCC 2 cut(s) 256, 268
DpnI GATC 4 cut(s) 131, 137, 147, 398
DpnII GATC 4 cut(s) 129, 135, 145, 396
DrdI GACNNNNNNGTC 1 cut(s) 641
DriI GACNNNNNGTC 2 cut(s) 46, 298
DseDI GACNNNNNNGTC 1 cut(s) 641
Eam1105I GACNNNNNGTC 2 cut(s) 46, 298
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 345, 513
Eco24I GRGCYC 2 cut(s) 23, 121
Eco31I GGTCTC 1 cut(s) 33
Eco47I GGWCC 4 cut(s) 102, 292, 412, 507
Eco81I CCTNAGG 1 cut(s) 651
Eco88I CYCGRG 3 cut(s) 287, 314, 371
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 294, 518
EcoT14I CCWWGG 2 cut(s) 345, 513
EcoT38I GRGCYC 2 cut(s) 23, 121
EgeI GGCGCC 2 cut(s) 256, 268
EheI GGCGCC 2 cut(s) 256, 268
ErhI CCWWGG 2 cut(s) 345, 513
Esp3I CGTCTC 2 cut(s) 201, 283
FaeI CATG 2 cut(s) 424, 674
FaiI YATR 5 cut(s) 391, 404, 422, 672, 684
FaqI GGGAC 4 cut(s) 64, 115, 219, 286
FatI CATG 2 cut(s) 420, 670
FauI CCCGC 2 cut(s) 90, 92
Fnu4HI GCNGC 4 cut(s) 54, 57, 368, 481
FokI GGATG 3 cut(s) 203, 269, 611
FriOI GRGCYC 2 cut(s) 23, 121
Fsp4HI GCNGC 4 cut(s) 54, 57, 368, 481
GlaI GCGC 6 cut(s) 86, 163, 256, 268, 466, 702
GluI GCNGC 4 cut(s) 54, 57, 368, 481
GsaI CCCAGC 3 cut(s) 99, 124, 307
HaeII RGCGCY 4 cut(s) 165, 258, 270, 468
HaeIII GGCC 5 cut(s) 182, 350, 380, 459, 518
HapII CCGG 5 cut(s) 36, 114, 204, 484, 662
HgaI GACGC 5 cut(s) 218, 232, 394, 620, 683
HhaI GCGC 6 cut(s) 87, 164, 257, 269, 467, 703
Hin1I GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
Hin1II CATG 2 cut(s) 424, 674
Hin6I GCGC 6 cut(s) 85, 162, 255, 267, 465, 701
HinP1I GCGC 6 cut(s) 85, 162, 255, 267, 465, 701
HinfI GANTC 2 cut(s) 299, 489
HpaII CCGG 5 cut(s) 36, 114, 204, 484, 662
HphI GGTGA 2 cut(s) 328, 334
Hpy188I TCNGA 2 cut(s) 31, 589
Hpy188III TCNNGA 6 cut(s) 114, 133, 187, 472, 577, 671
Hpy99I CGWCG 9 cut(s) 79, 129, 146, 158, 278, 473, 578, 614, 647
HpyAV CCTTC 2 cut(s) 175, 418
HpyCH4III ACNGT 2 cut(s) 75, 340
HpyCH4IV ACGT 2 cut(s) 228, 276
HpyCH4V TGCA 2 cut(s) 550, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 698
HpyF3I CTNAG 4 cut(s) 149, 461, 501, 651
HpySE526I ACGT 2 cut(s) 228, 276
Hsp92I GRCGYC 6 cut(s) 210, 255, 267, 276, 386, 612
Hsp92II CATG 2 cut(s) 424, 674
HspAI GCGC 6 cut(s) 85, 162, 255, 267, 465, 701
KasI GGCGCC 2 cut(s) 254, 266
KflI GGGWCCC 1 cut(s) 102
Kpn2I TCCGGA 1 cut(s) 113
Kzo9I GATC 4 cut(s) 129, 135, 145, 396
LmnI GCTCC 3 cut(s) 116, 427, 658
Lsp1109I GCAGC 2 cut(s) 40, 492
LweI GCATC 1 cut(s) 679
MaeII ACGT 2 cut(s) 228, 276
MaeIII GTNAC 3 cut(s) 340, 536, 626
MalI GATC 4 cut(s) 131, 137, 147, 398
MbiI CCGCTC 2 cut(s) 370, 430
MboI GATC 4 cut(s) 129, 135, 145, 396
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 23, 121
MluCI AATT 1 cut(s) 331
Mly113I GGCGCC 2 cut(s) 255, 267
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 94
MroI TCCGGA 1 cut(s) 113
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 2 cut(s) 575, 687
MspA1I CMGCKG 2 cut(s) 53, 99
MspI CCGG 5 cut(s) 36, 114, 204, 484, 662
MspR9I CCNGG 5 cut(s) 36, 204, 296, 485, 520
MvaI CCWGG 2 cut(s) 296, 520
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 2 cut(s) 159, 698
NarI GGCGCC 2 cut(s) 255, 267
NciI CCSGG 3 cut(s) 36, 204, 485
NdeII GATC 4 cut(s) 129, 135, 145, 396
NlaIII CATG 2 cut(s) 424, 674
NlaIV GGNNCC 7 cut(s) 33, 103, 104, 118, 256, 268, 660
NmeAIII GCCGAG 1 cut(s) 504
NmuCI GTSAC 2 cut(s) 340, 536
PaeR7I CTCGAG 2 cut(s) 314, 371
PagI TCATGA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 150
PfeI GAWTC 1 cut(s) 489
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 4 cut(s) 55, 58, 369, 482
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 2 cut(s) 258, 270
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 294, 518
PspFI CCCAGC 3 cut(s) 95, 120, 303
PspGI CCWGG 2 cut(s) 294, 518
PspN4I GGNNCC 7 cut(s) 33, 103, 104, 118, 256, 268, 660
PspPI GGNCC 5 cut(s) 102, 180, 292, 412, 507
PspPPI RGGWCCY 1 cut(s) 102
PspXI VCTCGAGB 2 cut(s) 314, 371
PsuI RGATCY 1 cut(s) 396
PsyI GACNNNGTC 1 cut(s) 212
RseI CAYNNNNRTG 2 cut(s) 575, 687
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 4 cut(s) 54, 57, 368, 481
Sau3AI GATC 4 cut(s) 129, 135, 145, 396
Sau96I GGNCC 5 cut(s) 102, 180, 292, 412, 507
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 5 cut(s) 36, 204, 296, 485, 520
SduI GDGCHC 2 cut(s) 23, 121
SfaNI GCATC 1 cut(s) 679
SfoI GGCGCC 2 cut(s) 256, 268
Sfr274I CTCGAG 2 cut(s) 314, 371
SinI GGWCC 4 cut(s) 102, 292, 412, 507
SlaI CTCGAG 2 cut(s) 314, 371
SmiMI CAYNNNNRTG 2 cut(s) 575, 687
SmlI CTYRAG 2 cut(s) 314, 371
SmoI CTYRAG 2 cut(s) 314, 371
Sse9I AATT 1 cut(s) 331
SsiI CCGC 9 cut(s) 43, 51, 56, 83, 99, 199, 368, 430, 505
SspDI GGCGCC 2 cut(s) 254, 266
StyD4I CCNGG 5 cut(s) 34, 202, 294, 483, 518
StyI CCWWGG 2 cut(s) 345, 513
TaaI ACNGT 2 cut(s) 75, 340
TaiI ACGT 2 cut(s) 231, 279
TaqI TCGA 4 cut(s) 144, 315, 327, 372
TasI AATT 1 cut(s) 331
TauI GCSGC 2 cut(s) 59, 370
TfiI GAWTC 1 cut(s) 489
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 3 cut(s) 535, 541, 560
TseFI GTSAC 2 cut(s) 340, 536
TseI GCWGC 2 cut(s) 53, 480
Tsp45I GTSAC 2 cut(s) 340, 536
TspDTI ATGAA 3 cut(s) 437, 618, 659
TspGWI ACGGA 2 cut(s) 142, 221
TspRI CASTG 3 cut(s) 535, 541, 560
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 4 cut(s) 102, 292, 412, 507
XhoI CTCGAG 2 cut(s) 314, 371
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.