Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000851

Physical Location & Seq

Genomic Coordinates Reverse (-)

4625308 .. 4628980

Gene Structure 3673 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1054300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGACTCAGACGTCGACGTTTCGCGGTGGGTCATCGAGCTCCTTCTCCGGGACCGCGAAAAGGATTGCATTGCCAAGAGGGTCCTCGCAGTCGCACCCTTCTCGGACCAAGACTATCGCTTGAAGAAGACGGTACTCCTCCGAACCATCGAGTGCGAGGTATACGAAGCTTCGGTCTCCGAAACGATCCTCGAAACGCTTGAAATGATTGAGGATTTGGATATCAGAGAAGGCATTTCCACCATGGAATCCATGAAAGCCGCCTACTGCGCCGTCGCCACTGAGTGTACTGTCAAGTTCTTGGTGGGTTTTATGGGAAAGCCTAGTGGAAAGTACTTGGAGGCCGTGGATCGCATTTGGAGAGGGAGGGTTGGGGTTTTGGAGCGGTCTGGGAGGAGCCAGCTGGTATCGGCGGAGTTGAGGCATCGAATGGATGAGGTCGAGGCAGGGGTTTGGGATTTGAGAGTGTCGAAGAAGCTGTCGAGGATGAACACGAGGAATGATGCGCTGAGGTTGCTTGCAGTTTATGTGAAAGCAGCTTTGGCGTTGTTGGGACCTCCGTTTATTACTTGGGCTATTAGATTGAATGTGCCCAAGGAAGTTGCCGACTTGGAAGCTGATCAGATTACAGTGGCCAATGAGGCTAATGTTGGTTGTGGGTCTGAAGTGGAGGGGCAAAAGAAGAAGGGGGCTTATGCGGGTGACAGGACTGAATTGGAGGTACAAATGGCGAATGGGGATAATGTGGGGGCAGGTGTGTCTGAATTGGAGAATCAAATGGCGAATGGGGATAATGCGGGGGCAGGTGTGTCTGAATTGGGGAGTCAAATGGCGAATAGGGCTAACGTGGGTGGTGATGGTGGGTCTGAATTGGATGCTCAAATGGCAAACCAGGCAAATGTGGGGGTGCGTGGGTTGGATTTAGAGGCTGAAACGGAGAATGAGACTACTGTGGGTGGTCGGTCTGATATGGAGGTGCAAATGGAGAATGGGACTGATGCGGGTGATGGATCTGAATTGGGGGAGCAAATGGTTAATGGGACTAATGTGGGTGACGGGGATCAATTGGAGGAGCAAACTAGGAAGGAGGCTAATGTGACTGGTGACTCTGAATTGGAAGCACAAATGATGAGTGGAGCTAGTCTGGTTGATAGGTCCGAGCCAGAATCACAAACTGTTAATGGGACTAATGAGGGTGTTCGGTCACAAAGGCAGGCACAAACTACGAATAGATCTAATGTGGGCAGTCAGTCTGCATTGGAAGAGCAACAATTGAATGCGATAAATTTCGGTGAGGAGTCTGAATTGGGTCCGATATTAGTTGCCGTCCCATTGAAGGAAGTTCGGGCAGGGAGCTCGCCTAGTCTGAATGGGGAACTGGATCTCAGTATTGACCATACCACGAAGGATAAAGAAAATCAAAGTGCTGTGCCTAGACACAAGCACCTTGCTCATAGACGCACTAGAGGACCAATTAGGATTGGGGATGCTGAAGATTCAAGCACAGAGGTATCAAATAGAAAATTTGGTTCCCTTTCTTCCCCTGAAGTTAACAAAGCACAAGAAGCACTTAAATCCAGTATTTCGGAGCTCCAAGAAGTAGTGACTGATCCACTTCCAGATGCATTACGAGTGTCTGAAACCATAAGATCTGGTTTGGCAGTGAGATCTGCGAATCATGTAAATGGAAAAGATGTAGGTGCAGATAATCCATCTATCGAGAAGGGTGCTGGGCCTGTTCAATCTAGTAATGATAATGTTGGAAATCTGCCATCCAGACATCAGAATGATCCTCAATCTAGTGGAATAGAAAAGGATGTACCAAATCCGCCTGTCGACCAGGGAGGCACTGAGTCTGTTCAAACTGATGCTGCCAATCTTAGAAATGCTTCTCTCAATAATCAGAATAACGTTGCACGTTCTAGCTTGATGGAACGTAATAGTACTGCCCGCACTTTCGAGTGGGAGGACTCAATAGATACGTCACAAGAAGGAACAAAGAAAGATGCAGGCAGGCTTCACCTTCCGAGCCCAAAGAGGACTGCTGTTTCTCCATTGAAGAAGTATGGGAATATCAGATTGACTAAGAGAAGAAAAGTTAAGAGATGGAGCTTATTGGAAGAAGAAACCTTAAGGACTGGTGTACAGAAGTATGGAAAAGGGAACTGGAAACTTATCTTGAATAGCTACCGCGATATATTCGAAGAGAGAACTGAGGTTGATCTAAAGGACAAGTGGAGAAATATGGAAGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAO Myb_DNA-binding

Protein Analysis

752

Amino Acids

81.9

Weight (kDa)

4.98

Isoelectric Point (pI)

46.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	702 - 749	2.5e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06910 AT1G15720 AT5G58340 AT5G58340
fragaria_vesca	FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_3g40451 FvH4_5g00450 FvH4_5g00450 FvH4_5g27240 FvH4_5g27240 FvH4_5g27240 FvH4_5g27240
malus_domestica	MD03G1052200.v1.1 MD10G1106900.v1.1 MD11G1054300.v1.1
prunus_persica	Prupe.6G041700_v2.0.a1
pyrus_communis	pycom03g04150 pycom11g04410
rosa_chinensis	RchiOBHm_Chr5g0000441 RchiOBHm_Chr5g0073101 RchiOBHm_Chr7g0218431 RchiOBHm_Chr7g0221531 RchiOBHm_Chr7g0221541
rosa_laevigata	RLG00000002155 RLG00000002374 RLG00000030867 RLG00000036363
rosa_multiflora	Rmu_co8212148.1_g000001 Rmu_co8273167.1_g000001 Rmu_sc0001680.1_g000002 Rmu_sc0003949.1_g000004 Rmu_sc0005592.1_g000024 Rmu_sc0005994.1_g000013 Rmu_sc0029255.1_g000001 Rmu_sc0029256.1_g000001 Rmu_ssc0000385.1_g000016
rosa_roxburghii	Rroxscaffold_1G00008100 Rroxscaffold_1G00008150 Rroxscaffold_1G00075720 Rroxscaffold_3G00240990 Rroxscaffold_5G00361570
rosa_rugosa	Rorug04G0383600.1 Rorug04G0383700 Rorug05G0421100 Rorug07G0177000
rosa_samantha	Rh5AG003600 Rh5AG478100 Rh5BG004800 Rh5BG498800 Rh5CG003900 Rh5CG522000 Rh5DG003700 Rh5DG510900 Rh7AG318400 Rh7BG310300 Rh7CG335200 Rh7CG335300 Rh7DG317400
rosa_wichuraiana	Rw5G000360 Rw5G044400 Rw7G027060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	743, 794
AasI	GACNNNNNNGTC	1 cut(s)	11
AatII	GACGTC	1 cut(s)	15
Acc36I	ACCTGC	2 cut(s)	743, 794
AccBSI	CCGCTC	1 cut(s)	385
AccI	GTMKAC	3 cut(s)	15, 162, 1836
AccII	CGCG	3 cut(s)	25, 57, 2193
AclI	AACGTT	1 cut(s)	1911
AclWI	GGATC	7 cut(s)	181, 357, 1018, 1068, 1389, 1604, 1784
AcoI	YGGCCR	1 cut(s)	633
AcsI	RAATTY	2 cut(s)	1285, 1523
AcuI	CTGAAG	3 cut(s)	684, 1512, 1566
AcyI	GRCGYC	1 cut(s)	12
AdeI	CACNNNGTG	1 cut(s)	285
AfaI	GTAC	7 cut(s)	135, 289, 335, 723, 1821, 1945, 2145
AfiI	CCNNNNNNNGG	3 cut(s)	49, 61, 1336
AflII	CTTAAG	1 cut(s)	2131
AjnI	CCWGG	2 cut(s)	891, 1839
AjuI	GAANNNNNNNTTGG	2 cut(s)	524, 556
Alw21I	GWGCWC	3 cut(s)	42, 1358, 1593
Alw26I	GTCTC	2 cut(s)	181, 938
AlwI	GGATC	7 cut(s)	181, 357, 1018, 1068, 1389, 1604, 1784
AoxI	GGCC	3 cut(s)	342, 633, 1733
ApeKI	GCWGC	2 cut(s)	536, 1871
ApoI	RAATTY	2 cut(s)	1285, 1523
ArsI	GACNNNNNNTTYG	4 cut(s)	159, 191, 464, 496
Asp700I	GAANNNNTTC	1 cut(s)	1888
AspLEI	GCGC	2 cut(s)	272, 508
AspS9I	GGNCC	8 cut(s)	52, 82, 106, 554, 1155, 1310, 1469, 1733
AsuC2I	CCSGG	1 cut(s)	50
AsuHPI	GGTGA	7 cut(s)	713, 866, 1016, 1064, 1115, 1304, 2012
AsuII	TTCGAA	1 cut(s)	2202
AvaII	GGWCC	7 cut(s)	52, 82, 106, 554, 1155, 1310, 1469
BaeGI	GKGCMC	1 cut(s)	594
BaeI	ACNNNNGTAYC	2 cut(s)	1494, 1527
BalI	TGGCCA	1 cut(s)	635
BanII	GRGCYC	4 cut(s)	42, 1358, 1593, 2033
BauI	CACGAG	1 cut(s)	493
BbsI	GAAGAC	1 cut(s)	134
Bbv12I	GWGCWC	3 cut(s)	42, 1358, 1593
BbvCI	CCTCAGC	1 cut(s)	509
BbvI	GCAGC	2 cut(s)	548, 1858
BccI	CCATC	7 cut(s)	155, 851, 1001, 1720, 1780, 1924, 2100
BceAI	ACGGC	3 cut(s)	257, 329, 1310
BcgI	CGANNNNNNTGC	6 cut(s)	84, 118, 1338, 1372, 1709, 1743
BciT130I	CCWGG	2 cut(s)	893, 1841
BclI	TGATCA	1 cut(s)	619
BcnI	CCSGG	1 cut(s)	50
BcoDI	GTCTC	2 cut(s)	181, 938
BfaI	CTAG	9 cut(s)	324, 1080, 1140, 1362, 1434, 1464, 1746, 1800, 1923
BfrI	CTTAAG	1 cut(s)	2131
BfuAI	ACCTGC	2 cut(s)	743, 794
BglI	GCCNNNNNGGC	1 cut(s)	641
BglII	AGATCT	3 cut(s)	1232, 1649, 1667
BisI	GCNGC	3 cut(s)	261, 537, 1872
BlsI	GCNGC	3 cut(s)	262, 538, 1873
BmcAI	AGTACT	2 cut(s)	335, 1945
Bme1390I	CCNGG	3 cut(s)	50, 893, 1841
Bme18I	GGWCC	7 cut(s)	52, 82, 106, 554, 1155, 1310, 1469
BmgT120I	GGNCC	8 cut(s)	52, 82, 106, 554, 1155, 1310, 1469, 1733
BmiI	GGNNCC	6 cut(s)	53, 83, 398, 555, 1311, 1531
BmrFI	CCNGG	3 cut(s)	50, 893, 1841
BmsI	GCATC	8 cut(s)	433, 493, 865, 988, 1477, 1612, 1858, 1996
BpiI	GAAGAC	1 cut(s)	134
Bpu10I	CCTNAGC	1 cut(s)	509
Bpu14I	TTCGAA	1 cut(s)	2202
BpuMI	CCSGG	1 cut(s)	50
BsaHI	GRCGYC	1 cut(s)	12
BsaI	GGTCTC	1 cut(s)	181
BsaJI	CCNNGG	4 cut(s)	243, 345, 594, 1840
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 61, 1336
Bse1I	ACTGG	5 cut(s)	1105, 1383, 1578, 2143, 2171
Bse3DI	GCAATG	1 cut(s)	69
BseBI	CCWGG	2 cut(s)	893, 1841
BseDI	CCNNGG	4 cut(s)	243, 345, 594, 1840
BseGI	GGATG	6 cut(s)	439, 492, 880, 1492, 1772, 1822
BseLI	CCNNNNNNNGG	3 cut(s)	49, 61, 1336
BseMI	GCAATG	1 cut(s)	69
BseMII	CTCAG	6 cut(s)	21, 273, 500, 1399, 1842, 2205
BseNI	ACTGG	5 cut(s)	1105, 1383, 1578, 2143, 2171
BseRI	GAGGAG	4 cut(s)	128, 409, 1085, 1310
BseSI	GKGCMC	1 cut(s)	594
BseXI	GCAGC	2 cut(s)	548, 1858
BseYI	CCCAGC	1 cut(s)	1730
BsgI	GTGCAG	1 cut(s)	1722
Bsh1236I	CGCG	3 cut(s)	25, 57, 2193
BshFI	GGCC	3 cut(s)	344, 635, 1735
BsiHKAI	GWGCWC	3 cut(s)	42, 1358, 1593
BsiSI	CCGG	1 cut(s)	49
BslFI	GGGAC	6 cut(s)	65, 567, 1006, 1054, 1198, 1313
BslI	CCNNNNNNNGG	3 cut(s)	49, 61, 1336
BsmAI	GTCTC	2 cut(s)	181, 938
BsmFI	GGGAC	6 cut(s)	65, 567, 1006, 1054, 1198, 1313
BsmI	GAATGC	1 cut(s)	1282
BsnI	GGCC	3 cut(s)	344, 635, 1735
Bso31I	GGTCTC	1 cut(s)	181
Bsp119I	TTCGAA	1 cut(s)	2202
Bsp1286I	GDGCHC	5 cut(s)	42, 594, 1358, 1593, 2033
Bsp1407I	TGTACA	1 cut(s)	2143
Bsp19I	CCATGG	1 cut(s)	243
BspANI	GGCC	3 cut(s)	344, 635, 1735
BspCNI	CTCAG	6 cut(s)	20, 274, 501, 1398, 1843, 2206
BspFNI	CGCG	3 cut(s)	25, 57, 2193
BspLI	GGNNCC	6 cut(s)	53, 83, 398, 555, 1311, 1531
BspMI	ACCTGC	2 cut(s)	743, 794
BspPI	GGATC	7 cut(s)	181, 357, 1018, 1068, 1389, 1604, 1784
BspQI	GCTCTTC	1 cut(s)	1257
BspT104I	TTCGAA	1 cut(s)	2202
BspTI	CTTAAG	1 cut(s)	2131
BspTNI	GGTCTC	1 cut(s)	181
BsrBI	CCGCTC	1 cut(s)	385
BsrDI	GCAATG	1 cut(s)	69
BsrGI	TGTACA	1 cut(s)	2143
BsrI	ACTGG	5 cut(s)	1105, 1383, 1578, 2143, 2171
BssECI	CCNNGG	4 cut(s)	243, 345, 594, 1840
BssNAI	GTATAC	1 cut(s)	163
BssNI	GRCGYC	1 cut(s)	12
BssSI	CACGAG	1 cut(s)	493
BssT1I	CCWWGG	2 cut(s)	243, 594
Bst1107I	GTATAC	1 cut(s)	163
Bst2BI	CACGAG	1 cut(s)	493
Bst2UI	CCWGG	2 cut(s)	893, 1841
Bst4CI	ACNGT	5 cut(s)	133, 292, 631, 952, 1177
Bst6I	CTCTTC	2 cut(s)	1257, 2199
BstACI	GRCGYC	1 cut(s)	12
BstAFI	CTTAAG	1 cut(s)	2131
BstAUI	TGTACA	1 cut(s)	2143
BstBI	TTCGAA	1 cut(s)	2202
BstC8I	GCNNGC	7 cut(s)	401, 519, 1215, 1358, 1951, 2011, 2015
BstDEI	CTNAG	8 cut(s)	7, 282, 509, 1385, 1851, 1880, 2085, 2214
BstDSI	CCRYGG	2 cut(s)	243, 345
BstF5I	GGATG	6 cut(s)	439, 492, 880, 1492, 1772, 1822
BstFNI	CGCG	3 cut(s)	25, 57, 2193
BstHHI	GCGC	2 cut(s)	272, 508
BstMAI	GTCTC	2 cut(s)	181, 938
BstMWI	GCNNNNNNNGC	8 cut(s)	269, 514, 542, 641, 839, 884, 893, 1565
BstNI	CCWGG	2 cut(s)	893, 1841
BstSCI	CCNGG	3 cut(s)	48, 891, 1839
BstSLI	GKGCMC	1 cut(s)	594
BstUI	CGCG	3 cut(s)	25, 57, 2193
BstV1I	GCAGC	2 cut(s)	548, 1858
BstV2I	GAAGAC	1 cut(s)	134
BstX2I	RGATCY	5 cut(s)	1010, 1232, 1381, 1649, 1667
BstYI	RGATCY	5 cut(s)	1010, 1232, 1381, 1649, 1667
BstZ17I	GTATAC	1 cut(s)	163
BsuRI	GGCC	3 cut(s)	344, 635, 1735
BtgI	CCRYGG	2 cut(s)	243, 345
BtsCI	GGATG	6 cut(s)	439, 492, 880, 1492, 1772, 1822
BtsI	GCAGTG	1 cut(s)	1668
BtsIMutI	CAGTG	4 cut(s)	279, 636, 1668, 1848
BveI	ACCTGC	2 cut(s)	743, 794
Cac8I	GCNNGC	7 cut(s)	401, 519, 1215, 1358, 1951, 2011, 2015
CfoI	GCGC	2 cut(s)	272, 508
Cfr13I	GGNCC	8 cut(s)	52, 82, 106, 554, 1155, 1310, 1469, 1733
CseI	GACGC	1 cut(s)	1467
Csp6I	GTAC	7 cut(s)	134, 288, 334, 722, 1820, 1944, 2144
CviAII	CATG	3 cut(s)	244, 253, 1679
CviQI	GTAC	7 cut(s)	134, 288, 334, 722, 1820, 1944, 2144
DdeI	CTNAG	8 cut(s)	7, 282, 509, 1385, 1851, 1880, 2085, 2214
DraIII	CACNNNGTG	1 cut(s)	285
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
EaeI	YGGCCR	1 cut(s)	633
Eam1104I	CTCTTC	2 cut(s)	1257, 2199
EarI	CTCTTC	2 cut(s)	1257, 2199
EciI	GGCGGA	2 cut(s)	428, 1818
Ecl136II	GAGCTC	3 cut(s)	40, 1356, 1591
Eco130I	CCWWGG	2 cut(s)	243, 594
Eco24I	GRGCYC	4 cut(s)	42, 1358, 1593, 2033
Eco31I	GGTCTC	1 cut(s)	181
Eco32I	GATATC	1 cut(s)	223
Eco47I	GGWCC	7 cut(s)	52, 82, 106, 554, 1155, 1310, 1469
Eco53kI	GAGCTC	3 cut(s)	40, 1356, 1591
Eco57I	CTGAAG	3 cut(s)	684, 1512, 1566
EcoICRI	GAGCTC	3 cut(s)	40, 1356, 1591
EcoO109I	RGGNCCY	2 cut(s)	82, 554
EcoRII	CCWGG	2 cut(s)	891, 1839
EcoRV	GATATC	1 cut(s)	223
EcoT14I	CCWWGG	2 cut(s)	243, 594
EcoT22I	ATGCAT	1 cut(s)	1627
EcoT38I	GRGCYC	4 cut(s)	42, 1358, 1593, 2033
ErhI	CCWWGG	2 cut(s)	243, 594
FaeI	CATG	3 cut(s)	247, 256, 1682
FalI	AAGNNNNNCTT	2 cut(s)	1554, 1586
FaqI	GGGAC	6 cut(s)	65, 567, 1006, 1054, 1198, 1313
FatI	CATG	3 cut(s)	243, 252, 1678
FauI	CCCGC	4 cut(s)	691, 790, 994, 1958
FbaI	TGATCA	1 cut(s)	619
FblI	GTMKAC	3 cut(s)	15, 162, 1836
Fnu4HI	GCNGC	3 cut(s)	261, 537, 1872
FokI	GGATG	6 cut(s)	446, 499, 887, 1499, 1759, 1829
FriOI	GRGCYC	4 cut(s)	42, 1358, 1593, 2033
Fsp4HI	GCNGC	3 cut(s)	261, 537, 1872
FspBI	CTAG	9 cut(s)	324, 1080, 1140, 1362, 1434, 1464, 1746, 1800, 1923
GlaI	GCGC	2 cut(s)	271, 507
GluI	GCNGC	3 cut(s)	261, 537, 1872
GsaI	CCCAGC	1 cut(s)	1734
HaeIII	GGCC	3 cut(s)	344, 635, 1735
HapII	CCGG	1 cut(s)	49
HgaI	GACGC	1 cut(s)	1467
HhaI	GCGC	2 cut(s)	272, 508
Hin1I	GRCGYC	1 cut(s)	12
Hin1II	CATG	3 cut(s)	247, 256, 1682
Hin6I	GCGC	2 cut(s)	270, 506
HinP1I	GCGC	2 cut(s)	270, 506
HincII	GTYRAC	3 cut(s)	16, 1552, 1837
HindII	GTYRAC	3 cut(s)	16, 1552, 1837
HindIII	AAGCTT	1 cut(s)	168
HpaI	GTTAAC	1 cut(s)	1552
HpaII	CCGG	1 cut(s)	49
HphI	GGTGA	7 cut(s)	713, 866, 1016, 1064, 1115, 1304, 2012
Hpy166II	GTNNAC	6 cut(s)	16, 163, 288, 1552, 1837, 2144
Hpy188III	TCNNGA	4 cut(s)	1619, 1720, 1776, 2179
Hpy8I	GTNNAC	6 cut(s)	16, 163, 288, 1552, 1837, 2144
Hpy99I	CGWCG	3 cut(s)	17, 20, 278
HpyCH4III	ACNGT	5 cut(s)	133, 292, 631, 952, 1177
HpyCH4IV	ACGT	7 cut(s)	12, 18, 846, 1911, 1918, 1936, 1982
HpyCH4V	TGCA	8 cut(s)	69, 521, 979, 1256, 1625, 1703, 1916, 2009
HpyF10VI	GCNNNNNNNGC	8 cut(s)	269, 514, 542, 641, 839, 884, 893, 1565
HpyF3I	CTNAG	8 cut(s)	7, 282, 509, 1385, 1851, 1880, 2085, 2214
HpySE526I	ACGT	7 cut(s)	12, 18, 846, 1911, 1918, 1936, 1982
Hsp92I	GRCGYC	1 cut(s)	12
Hsp92II	CATG	3 cut(s)	247, 256, 1682
HspAI	GCGC	2 cut(s)	270, 506
Ksp22I	TGATCA	1 cut(s)	619
KspAI	GTTAAC	1 cut(s)	1552
LguI	GCTCTTC	1 cut(s)	1257
Lsp1109I	GCAGC	2 cut(s)	548, 1858
LweI	GCATC	8 cut(s)	433, 493, 865, 988, 1477, 1612, 1858, 1996
MaeI	CTAG	9 cut(s)	324, 1080, 1140, 1362, 1434, 1464, 1746, 1800, 1923
MaeII	ACGT	7 cut(s)	12, 18, 846, 1911, 1918, 1936, 1982
MaeIII	GTNAC	7 cut(s)	701, 1052, 1096, 1103, 1203, 1603, 1983
MbiI	CCGCTC	1 cut(s)	385
MfeI	CAATTG	2 cut(s)	1064, 1271
MflI	RGATCY	5 cut(s)	1010, 1232, 1381, 1649, 1667
MhlI	GDGCHC	5 cut(s)	42, 594, 1358, 1593, 2033
MlsI	TGGCCA	1 cut(s)	635
MluNI	TGGCCA	1 cut(s)	635
MlyI	GAGTC	5 cut(s)	832, 1100, 1307, 1862, 1964
MmeI	TCCRAC	2 cut(s)	897, 1741
Mox20I	TGGCCA	1 cut(s)	635
Mph1103I	ATGCAT	1 cut(s)	1627
MroXI	GAANNNNTTC	1 cut(s)	1888
MscI	TGGCCA	1 cut(s)	635
MseI	TTAA	6 cut(s)	1035, 1179, 1551, 1572, 2100, 2132
MslI	CAYNNNNRTG	2 cut(s)	1683, 1785
Msp20I	TGGCCA	1 cut(s)	635
MspA1I	CMGCKG	1 cut(s)	403
MspCI	CTTAAG	1 cut(s)	2131
MspI	CCGG	1 cut(s)	49
MspR9I	CCNGG	3 cut(s)	50, 893, 1841
MunI	CAATTG	2 cut(s)	1064, 1271
Mva1269I	GAATGC	1 cut(s)	1282
MvaI	CCWGG	2 cut(s)	893, 1841
MvnI	CGCG	3 cut(s)	25, 57, 2193
MwoI	GCNNNNNNNGC	8 cut(s)	269, 514, 542, 641, 839, 884, 893, 1565
NciI	CCSGG	1 cut(s)	50
NcoI	CCATGG	1 cut(s)	243
NlaIII	CATG	3 cut(s)	247, 256, 1682
NlaIV	GGNNCC	6 cut(s)	53, 83, 398, 555, 1311, 1531
NmuCI	GTSAC	7 cut(s)	701, 1052, 1096, 1103, 1203, 1603, 1983
NsiI	ATGCAT	1 cut(s)	1627
NspV	TTCGAA	1 cut(s)	2202
PaqCI	CACCTGC	2 cut(s)	743, 794
PciSI	GCTCTTC	1 cut(s)	1257
PctI	GAATGC	1 cut(s)	1282
PdmI	GAANNNNTTC	1 cut(s)	1888
PfeI	GAWTC	5 cut(s)	248, 772, 1166, 1496, 1675
PfoI	TCCNGGA	1 cut(s)	48
PkrI	GCNGC	3 cut(s)	262, 538, 1873
PleI	GAGTC	5 cut(s)	831, 1100, 1306, 1861, 1964
PpsI	GAGTC	5 cut(s)	831, 1100, 1306, 1861, 1964
PpuMI	RGGWCCY	2 cut(s)	82, 554
Psp124BI	GAGCTC	3 cut(s)	42, 1358, 1593
Psp1406I	AACGTT	1 cut(s)	1911
Psp5II	RGGWCCY	2 cut(s)	82, 554
Psp6I	CCWGG	2 cut(s)	891, 1839
PspFI	CCCAGC	1 cut(s)	1730
PspGI	CCWGG	2 cut(s)	891, 1839
PspN4I	GGNNCC	6 cut(s)	53, 83, 398, 555, 1311, 1531
PspPI	GGNCC	8 cut(s)	52, 82, 106, 554, 1155, 1310, 1469, 1733
PspPPI	RGGWCCY	2 cut(s)	82, 554
PsuI	RGATCY	5 cut(s)	1010, 1232, 1381, 1649, 1667
PvuII	CAGCTG	1 cut(s)	403
RsaI	GTAC	7 cut(s)	135, 289, 335, 723, 1821, 1945, 2145
RsaNI	GTAC	7 cut(s)	134, 288, 334, 722, 1820, 1944, 2144
RseI	CAYNNNNRTG	2 cut(s)	1683, 1785
SacI	GAGCTC	3 cut(s)	42, 1358, 1593
SalI	GTCGAC	2 cut(s)	14, 1835
SapI	GCTCTTC	1 cut(s)	1257
SaqAI	TTAA	6 cut(s)	1035, 1179, 1551, 1572, 2100, 2132
SatI	GCNGC	3 cut(s)	261, 537, 1872
Sau96I	GGNCC	8 cut(s)	52, 82, 106, 554, 1155, 1310, 1469, 1733
ScaI	AGTACT	2 cut(s)	335, 1945
SchI	GAGTC	5 cut(s)	832, 1100, 1307, 1862, 1964
ScrFI	CCNGG	3 cut(s)	50, 893, 1841
SduI	GDGCHC	5 cut(s)	42, 594, 1358, 1593, 2033
SfaNI	GCATC	8 cut(s)	433, 493, 865, 988, 1477, 1612, 1858, 1996
SfuI	TTCGAA	1 cut(s)	2202
SgrDI	CGTCGACG	1 cut(s)	14
SinI	GGWCC	7 cut(s)	52, 82, 106, 554, 1155, 1310, 1469
SmiMI	CAYNNNNRTG	2 cut(s)	1683, 1785
SmlI	CTYRAG	1 cut(s)	2131
SmoI	CTYRAG	1 cut(s)	2131
SspMI	CTAG	9 cut(s)	324, 1080, 1140, 1362, 1434, 1464, 1746, 1800, 1923
SstI	GAGCTC	3 cut(s)	42, 1358, 1593
StyD4I	CCNGG	3 cut(s)	48, 891, 1839
StyI	CCWWGG	2 cut(s)	243, 594
TaaI	ACNGT	5 cut(s)	133, 292, 631, 952, 1177
TaiI	ACGT	7 cut(s)	15, 21, 849, 1914, 1921, 1939, 1985
TaqII	GACCGA	3 cut(s)	163, 951, 1191
TatI	WGTACW	4 cut(s)	287, 333, 1943, 2143
TauI	GCSGC	1 cut(s)	263
TfiI	GAWTC	5 cut(s)	248, 772, 1166, 1496, 1675
Tru1I	TTAA	6 cut(s)	1035, 1179, 1551, 1572, 2100, 2132
Tru9I	TTAA	6 cut(s)	1035, 1179, 1551, 1572, 2100, 2132
TscAI	CASTG	4 cut(s)	286, 636, 1668, 1855
TseFI	GTSAC	7 cut(s)	701, 1052, 1096, 1103, 1203, 1603, 1983
TseI	GCWGC	2 cut(s)	536, 1871
Tsp45I	GTSAC	7 cut(s)	701, 1052, 1096, 1103, 1203, 1603, 1983
TspDTI	ATGAA	2 cut(s)	269, 503
TspGWI	ACGGA	2 cut(s)	549, 950
TspRI	CASTG	4 cut(s)	286, 636, 1668, 1855
Vha464I	CTTAAG	1 cut(s)	2131
VpaK11BI	GGWCC	7 cut(s)	52, 82, 106, 554, 1155, 1310, 1469
XapI	RAATTY	2 cut(s)	1285, 1523
XmiI	GTMKAC	3 cut(s)	15, 162, 1836
XmnI	GAANNNNTTC	1 cut(s)	1888
XspI	CTAG	9 cut(s)	324, 1080, 1140, 1362, 1434, 1464, 1746, 1800, 1923
ZraI	GACGTC	1 cut(s)	13
ZrmI	AGTACT	2 cut(s)	335, 1945
Zsp2I	ATGCAT	1 cut(s)	1627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.