MD12G1035700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001542
Physical Location & Seq
Reverse (-)
3878130 .. 3880211
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035700.v1.1.491

Sequence Viewer

Length: 2082 bp
ATGAATTTTCAACAAGCTTGCCGTTTTGGCGGGTACATGCTCAAGGACTCCACCAGCTTGGCGCAAACCATCCAAACCTACGCCCGGACCAAGCAGCTGCACAGAGGCAAAGAGCTCCACGCTCAGCTCCTCCGCAACGAGTACCCACTATGCATCTTTCTCGCCAACCATCTTCTGAACATGTATTCCAAATGCGGGGAGGTCGACTATGCGCTCAAACTGTTCGACACAATGCGGCACAGAAACTTGGTTTCTTGGACGGCAATGATAACTGGGTTTTCTCAGAACTTGAGGTTCTCGGAGACGTTAAAAACGTTTTCCCAGATGATGGTTGCCGGAGAGAAACCAACCCAGTTTGCATTTGCAAGTGTTATAAGAGCTTGTGTGTTTCTCGGGTCGATTGAGATAGGGAGGCAGATGCATTCTCTTGCTTTGAAGTTGGGGTTGGCTTGTGAGTTGTTTGTGGGTAGTAATTTGGCTGATATGTATTGGAAGTGTGGGGTGACGGTTGATGCTTGTAAGGTTTTTGAGGAGATGCCGTGTAAGGACGCGGTATCATGGACTTCGATGATTGATGGGTATGCGAAGAGTGGTGATTCTGAGGCAGCTTTACTAACTTATAAAATGATGATTGTTGATGGGATTGTTATAGATCAGCATGTTCTTTGTACTGCATTAAATGCTTGTTCTGCGTTGAAGGATTGTAAGTTCGGAAGGTGTCTTCATTCAACCGTTCTAAAGTTAGGACTTCAAACGGAGGTTTCAGTGGGCAACGCTCTGACTGATATGTACGCGAAAGCAGGAGATATGGAAAGTGCTTCAGATGTGTTCCGGATTGATTCTGAGTGTAGAAACATTGTGTCTTCTACCTCTCTGATCAACGGTTATGTTGAGATGGATGAAATCGAGAAGGCTTTTAGTTTGTTTGTTGACTTACATAGTTGGGGAGTTGAGCCTAATGAGTTCACTTTCTCTAGCTTGATCAAGGCTTGTGCCAACCAAGCTGCACTTGATCAAGGAATCCAGCTTCACGCTCAGGCAATTAAACTTAATCTTGATAGACACCCTTTCATTTCTACTGTTCTTGTTGATATGTATGGAAAATGCGGGTTGCTTGATGATTCGACCCAAGTGTTTCATGAGATAGCAAACCCGAATGAAGTTGCATGGAATTCATTGTTAGGTGTGCTTGCTCTTCATGGATTAGGAAAAGAAGCCTCGGAAACATTTAGTAGAATGGTACAAAGAGGAGTGAAGCCGAATGCAATAACATTTGTCAGTCTTTTAATAGGGTGTAGCCATGCTGGATTGGTAAAGGATGGTTTAAATTACTTTTATTCTATGGAGAAAACATATGGAATAGTTCCTAGAGATGAACATTACTCTTGCGTTATTGACTTGCTTGGCCGGGCGGGAAGGCTTAAAGAGGCTGAAGAATTCATAAACAGCATGCCAATTCAACCAAATGCTTTCGGGTGGTGCTCTTTCCTCGGAGCCTGCAGGATTCACGGTGATAAGAAGAGGGGAAAACTGGCAGCAGAAAAACTGATGCAGCTAGAACCTGAGAACATCGGAGCACATATTCTGCTGTCGAATTTATATGCCAAGGAGCAGCAATGGGAAGATGTGAGGAGTGTGAGGAAGATGTTGAGAGATGGCACCATGAAGAAATTGCCAGGGTATAGTTGGGTTGACGTTGGAAACAAAACGCATATCTTCGGAGCCGAAGATTGGTCTCATCCTCTGAAGAAAGAAATTTATGAAAAGCTTGATACTCTTCTTGATCAGATAAAGAAAGCTGGATATGTTCCACAGACAGATTTGATTCCACATGATATGGATGATCATGAGAAGGAAAAGGTTCTTCACCATCACAGCGAGAGGATAGCGATTGCATTTGCGTTAATAAGTATGCCTGCTGGGAAGCCGATCATTGTGAAGAAAAATATAAGGGTGTGCTTGGATTGCCATTCTGCAATCAAGTACATCTCCAAGGTGGTAGGGAGAAAGATTATCCTTAGGGATAATAGTCGGTTTCACCATTTCGCAGGCGGGCAGTGTTCGTGTGGAGATTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

694

Amino Acids

77.6

Weight (kDa)

7.1

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 82 - 128 3.1e-06 PPR repeat family
PPR_2 PF13041 183 - 229 8.2e-06 PPR repeat family
PPR PF01535 185 - 215 5.4e-07 PPR repeat
PPR_2 PF13041 284 - 333 1.1e-13 PPR repeat family
PPR_2 PF13041 385 - 433 2.5e-10 PPR repeat family
E_motif PF20431 503 - 565 2.9e-16 E motif
Eplus_motif PF20430 570 - 597 1.1e-06 E+ motif
DYW_deaminase PF14432 601 - 693 3.3e-39 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 374, 621
AccB1I GGYRCC 1 cut(s) 1658
AccB7I CCANNNNNTGG 1 cut(s) 328
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 551, 794
AccIII TCCGGA 1 cut(s) 831
AciI CCGC 8 cut(s) 30, 133, 195, 235, 551, 1107, 1412, 2052
AclI AACGTT 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 1405
AcsI RAATTY 5 cut(s) 4, 1171, 1436, 1594, 1755
AcuI CTGAAG 3 cut(s) 804, 1452, 1766
AfaI GTAC 6 cut(s) 35, 143, 670, 791, 1242, 1985
AfiI CCNNNNNNNGG 4 cut(s) 84, 195, 328, 1731
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 6 cut(s) 11, 436, 697, 729, 752, 1460
AjnI CCWGG 1 cut(s) 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AloI GAACNNNNNNTCC 4 cut(s) 170, 202, 692, 724
Alw21I GWGCWC 3 cut(s) 117, 1484, 1579
Alw26I GTCTC 2 cut(s) 296, 1740
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 831
AoxI GGCC 1 cut(s) 1405
ApeKI GCWGC 7 cut(s) 94, 97, 605, 1004, 1535, 1552, 1612
ApoI RAATTY 5 cut(s) 4, 1171, 1436, 1594, 1755
Asp700I GAANNNNTTC 1 cut(s) 1860
AspLEI GCGC 2 cut(s) 64, 214
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 2 cut(s) 85, 1409
AsuHPI GGTGA 5 cut(s) 514, 605, 1523, 1859, 2030
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 2018
BanI GGYRCC 1 cut(s) 1658
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 2 cut(s) 713, 855
Bbv12I GWGCWC 3 cut(s) 117, 1484, 1579
BbvI GCAGC 7 cut(s) 84, 106, 617, 991, 1547, 1564, 1624
BccI CCATC 9 cut(s) 77, 177, 322, 569, 632, 889, 1313, 1649, 1878
BceAI ACGGC 3 cut(s) 6, 276, 523
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 1677
BclI TGATCA 5 cut(s) 876, 981, 1012, 1783, 1843
BcnI CCSGG 2 cut(s) 85, 1409
BcoDI GTCTC 2 cut(s) 296, 1740
BfaI CTAG 3 cut(s) 975, 1368, 1556
BfmI CTRYAG 1 cut(s) 1498
BglI GCCNNNNNGGC 1 cut(s) 27
BisI GCNGC 8 cut(s) 95, 98, 236, 606, 1005, 1536, 1553, 1613
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 8 cut(s) 96, 99, 237, 607, 1006, 1537, 1554, 1614
Bme1390I CCNGG 3 cut(s) 85, 1409, 1677
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 1495, 1660, 1723
BmrFI CCNGG 3 cut(s) 85, 1409, 1677
BmrI ACTGGG 2 cut(s) 282, 346
BmsI GCATC 5 cut(s) 162, 408, 502, 525, 1539
BmuI ACTGGG 2 cut(s) 282, 346
BpiI GAAGAC 2 cut(s) 713, 855
Bpu10I CCTNAGC 1 cut(s) 1035
Bpu1102I GCTNAGC 1 cut(s) 123
BpuEI CTTGAG 2 cut(s) 26, 310
BpuMI CCSGG 2 cut(s) 85, 1409
BsaI GGTCTC 1 cut(s) 1740
BsaJI CCNNGG 5 cut(s) 1218, 1489, 1605, 1676, 1992
BsaWI WCCGGW 1 cut(s) 831
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 195, 328, 1731
Bse1I ACTGG 4 cut(s) 277, 352, 1536, 2081
Bse21I CCTNAGG 1 cut(s) 2018
Bse3DI GCAATG 2 cut(s) 270, 1622
BseAI TCCGGA 1 cut(s) 831
BseBI CCWGG 1 cut(s) 1677
BseDI CCNNGG 5 cut(s) 1218, 1489, 1605, 1676, 1992
BseGI GGATG 5 cut(s) 69, 904, 1324, 1738, 1846
BseLI CCNNNNNNNGG 4 cut(s) 84, 195, 328, 1731
BseMI GCAATG 2 cut(s) 270, 1622
BseMII CTCAG 6 cut(s) 137, 296, 591, 834, 1049, 1554
BseNI ACTGG 4 cut(s) 277, 352, 1536, 2081
BseRI GAGGAG 4 cut(s) 119, 545, 1263, 1645
BseXI GCAGC 7 cut(s) 84, 106, 617, 991, 1547, 1564, 1624
BseYI CCCAGC 1 cut(s) 1919
BsgI GTGCAG 2 cut(s) 83, 990
Bsh1236I CGCG 2 cut(s) 551, 794
BshFI GGCC 1 cut(s) 1407
BshNI GGYRCC 1 cut(s) 1658
BsiHKAI GWGCWC 3 cut(s) 117, 1484, 1579
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 4 cut(s) 85, 336, 832, 1408
BslI CCNNNNNNNGG 4 cut(s) 84, 195, 328, 1731
BsmAI GTCTC 2 cut(s) 296, 1740
BsmBI CGTCTC 1 cut(s) 296
BsmI GAATGC 2 cut(s) 421, 1267
BsnI GGCC 1 cut(s) 1407
Bso31I GGTCTC 1 cut(s) 1740
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 117, 1484, 1579
Bsp13I TCCGGA 1 cut(s) 831
Bsp143I GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
Bsp1720I GCTNAGC 1 cut(s) 123
BspACI CCGC 8 cut(s) 30, 133, 195, 235, 551, 1107, 1412, 2052
BspANI GGCC 1 cut(s) 1407
BspCNI CTCAG 6 cut(s) 136, 295, 592, 835, 1048, 1555
BspEI TCCGGA 1 cut(s) 831
BspFNI CGCG 2 cut(s) 551, 794
BspHI TCATGA 2 cut(s) 1138, 1846
BspLI GGNNCC 3 cut(s) 1495, 1660, 1723
BspMAI CTGCAG 1 cut(s) 1502
BspQI GCTCTTC 1 cut(s) 1200
BspT107I GGYRCC 1 cut(s) 1658
BspTNI GGTCTC 1 cut(s) 1740
BsrDI GCAATG 2 cut(s) 270, 1622
BsrI ACTGG 4 cut(s) 277, 352, 1536, 2081
BssECI CCNNGG 5 cut(s) 1218, 1489, 1605, 1676, 1992
BssMI GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
BssT1I CCWWGG 2 cut(s) 1605, 1992
Bst2UI CCWGG 1 cut(s) 1677
Bst4CI ACNGT 6 cut(s) 222, 508, 733, 884, 1081, 1511
Bst6I CTCTTC 4 cut(s) 581, 1200, 1514, 1782
BstAPI GCANNNNNTGC 1 cut(s) 680
BstC8I GCNNGC 7 cut(s) 19, 1191, 1451, 1498, 1917, 2050, 2054
BstDEI CTNAG 7 cut(s) 123, 282, 600, 843, 1035, 1563, 2018
BstF5I GGATG 5 cut(s) 69, 904, 1324, 1738, 1846
BstFNI CGCG 2 cut(s) 551, 794
BstHHI GCGC 2 cut(s) 64, 214
BstKTI GATC 7 cut(s) 655, 879, 984, 1015, 1786, 1846, 1932
BstMAI GTCTC 2 cut(s) 296, 1740
BstMBI GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
BstMWI GCNNNNNNNGC 5 cut(s) 27, 680, 689, 1001, 1965
BstNI CCWGG 1 cut(s) 1677
BstNSI RCATGY 4 cut(s) 40, 184, 662, 1453
BstSCI CCNGG 3 cut(s) 83, 1407, 1675
BstSFI CTRYAG 1 cut(s) 1498
BstUI CGCG 2 cut(s) 551, 794
BstV1I GCAGC 7 cut(s) 84, 106, 617, 991, 1547, 1564, 1624
BstV2I GAAGAC 2 cut(s) 713, 855
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu36I CCTNAGG 1 cut(s) 2018
BsuRI GGCC 1 cut(s) 1407
BtsCI GGATG 5 cut(s) 69, 904, 1324, 1738, 1846
BtsI GCAGTG 1 cut(s) 2063
BtsIMutI CAGTG 2 cut(s) 771, 2063
Cac8I GCNNGC 7 cut(s) 19, 1191, 1451, 1498, 1917, 2050, 2054
CciI TCATGA 2 cut(s) 1138, 1846
CfoI GCGC 2 cut(s) 64, 214
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 557
Csp6I GTAC 6 cut(s) 34, 142, 669, 790, 1241, 1984
CviQI GTAC 6 cut(s) 34, 142, 669, 790, 1241, 1984
DdeI CTNAG 7 cut(s) 123, 282, 600, 843, 1035, 1563, 2018
DpnI GATC 7 cut(s) 654, 878, 983, 1014, 1785, 1845, 1931
DpnII GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
DraI TTTAAA 1 cut(s) 1326
EaeI YGGCCR 1 cut(s) 1405
Eam1104I CTCTTC 4 cut(s) 581, 1200, 1514, 1782
EarI CTCTTC 4 cut(s) 581, 1200, 1514, 1782
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 1605, 1992
Eco24I GRGCYC 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 1740
Eco47I GGWCC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 3 cut(s) 804, 1452, 1766
Eco81I CCTNAGG 1 cut(s) 2018
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 115
EcoRI GAATTC 2 cut(s) 1171, 1436
EcoRII CCWGG 1 cut(s) 1675
EcoT14I CCWWGG 2 cut(s) 1605, 1992
EcoT22I ATGCAT 2 cut(s) 155, 423
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 2 cut(s) 1605, 1992
Esp3I CGTCTC 1 cut(s) 296
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FauI CCCGC 5 cut(s) 23, 188, 1100, 1405, 2045
FauNDI CATATG 1 cut(s) 1354
FbaI TGATCA 5 cut(s) 876, 981, 1012, 1783, 1843
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 95, 98, 236, 606, 1005, 1536, 1553, 1613
FokI GGATG 5 cut(s) 56, 911, 1331, 1725, 1853
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 8 cut(s) 95, 98, 236, 606, 1005, 1536, 1553, 1613
FspBI CTAG 3 cut(s) 975, 1368, 1556
GlaI GCGC 2 cut(s) 63, 213
GluI GCNGC 8 cut(s) 95, 98, 236, 606, 1005, 1536, 1553, 1613
GsaI CCCAGC 1 cut(s) 1923
HaeIII GGCC 1 cut(s) 1407
HapII CCGG 4 cut(s) 85, 336, 832, 1408
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 64, 214
Hin6I GCGC 2 cut(s) 62, 212
HinP1I GCGC 2 cut(s) 62, 212
HincII GTYRAC 3 cut(s) 205, 931, 1693
HindII GTYRAC 3 cut(s) 205, 931, 1693
HindIII AAGCTT 2 cut(s) 15, 1766
HinfI GANTC 7 cut(s) 47, 596, 839, 1020, 1121, 1504, 1825
HpaII CCGG 4 cut(s) 85, 336, 832, 1408
HphI GGTGA 5 cut(s) 514, 605, 1523, 1859, 2030
Hpy166II GTNNAC 4 cut(s) 205, 931, 966, 1693
Hpy188III TCNNGA 6 cut(s) 832, 907, 1055, 1139, 1781, 1847
Hpy8I GTNNAC 4 cut(s) 205, 931, 966, 1693
HpyAV CCTTC 5 cut(s) 691, 708, 904, 1410, 1846
HpyCH4III ACNGT 6 cut(s) 222, 508, 733, 884, 1081, 1511
HpyCH4IV ACGT 3 cut(s) 305, 314, 1695
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 680, 689, 1001, 1965
HpyF3I CTNAG 7 cut(s) 123, 282, 600, 843, 1035, 1563, 2018
HpySE526I ACGT 3 cut(s) 305, 314, 1695
HspAI GCGC 2 cut(s) 62, 212
Kpn2I TCCGGA 1 cut(s) 831
Ksp22I TGATCA 5 cut(s) 876, 981, 1012, 1783, 1843
Kzo9I GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
LguI GCTCTTC 1 cut(s) 1200
LmnI GCTCC 6 cut(s) 120, 132, 1493, 1574, 1609, 1721
Lsp1109I GCAGC 7 cut(s) 84, 106, 617, 991, 1547, 1564, 1624
LweI GCATC 5 cut(s) 162, 408, 502, 525, 1539
MaeI CTAG 3 cut(s) 975, 1368, 1556
MaeII ACGT 3 cut(s) 305, 314, 1695
MaeIII GTNAC 1 cut(s) 502
MalI GATC 7 cut(s) 654, 878, 983, 1014, 1785, 1845, 1931
MboI GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
MhlI GDGCHC 3 cut(s) 117, 1484, 1579
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 1678
Mph1103I ATGCAT 2 cut(s) 155, 423
MroI TCCGGA 1 cut(s) 831
MroXI GAANNNNTTC 1 cut(s) 1860
MseI TTAA 8 cut(s) 308, 677, 1044, 1050, 1286, 1325, 1422, 1904
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 4 cut(s) 85, 336, 832, 1408
MspR9I CCNGG 3 cut(s) 85, 1409, 1677
Mva1269I GAATGC 2 cut(s) 421, 1267
MvaI CCWGG 1 cut(s) 1677
MvnI CGCG 2 cut(s) 551, 794
MwoI GCNNNNNNNGC 5 cut(s) 27, 680, 689, 1001, 1965
NciI CCSGG 2 cut(s) 85, 1409
NdeI CATATG 1 cut(s) 1354
NdeII GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
NlaIV GGNNCC 3 cut(s) 1495, 1660, 1723
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 2 cut(s) 155, 423
NspI RCATGY 4 cut(s) 40, 184, 662, 1453
PaeI GCATGC 1 cut(s) 1453
PagI TCATGA 2 cut(s) 1138, 1846
PciI ACATGT 1 cut(s) 180
PciSI GCTCTTC 1 cut(s) 1200
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PctI GAATGC 2 cut(s) 421, 1267
PdmI GAANNNNTTC 1 cut(s) 1860
PfeI GAWTC 6 cut(s) 596, 839, 1020, 1121, 1504, 1825
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 8 cut(s) 96, 99, 237, 607, 1006, 1537, 1554, 1614
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PscI ACATGT 1 cut(s) 180
PsiI TTATAA 2 cut(s) 374, 621
Psp124BI GAGCTC 1 cut(s) 117
Psp1406I AACGTT 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 1675
PspFI CCCAGC 1 cut(s) 1919
PspGI CCWGG 1 cut(s) 1675
PspN4I GGNNCC 3 cut(s) 1495, 1660, 1723
PspPI GGNCC 1 cut(s) 87
PstI CTGCAG 1 cut(s) 1502
PvuII CAGCTG 1 cut(s) 97
RsaI GTAC 6 cut(s) 35, 143, 670, 791, 1242, 1985
RsaNI GTAC 6 cut(s) 34, 142, 669, 790, 1241, 1984
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 1200
SaqAI TTAA 8 cut(s) 308, 677, 1044, 1050, 1286, 1325, 1422, 1904
SatI GCNGC 8 cut(s) 95, 98, 236, 606, 1005, 1536, 1553, 1613
Sau3AI GATC 7 cut(s) 652, 876, 981, 1012, 1783, 1843, 1929
Sau96I GGNCC 1 cut(s) 87
SbfI CCTGCAGG 1 cut(s) 1502
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 85, 1409, 1677
SdaI CCTGCAGG 1 cut(s) 1502
SduI GDGCHC 3 cut(s) 117, 1484, 1579
SfaNI GCATC 5 cut(s) 162, 408, 502, 525, 1539
SfcI CTRYAG 1 cut(s) 1498
SinI GGWCC 1 cut(s) 87
SmlI CTYRAG 2 cut(s) 41, 289
SmoI CTYRAG 2 cut(s) 41, 289
SphI GCATGC 1 cut(s) 1453
Sse8387I CCTGCAGG 1 cut(s) 1502
SsiI CCGC 8 cut(s) 30, 133, 195, 235, 551, 1107, 1412, 2052
SspMI CTAG 3 cut(s) 975, 1368, 1556
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 3 cut(s) 83, 1407, 1675
StyI CCWWGG 2 cut(s) 1605, 1992
TaaI ACNGT 6 cut(s) 222, 508, 733, 884, 1081, 1511
TaiI ACGT 3 cut(s) 308, 317, 1698
TaqI TCGA 7 cut(s) 204, 225, 398, 566, 906, 1124, 1592
TatI WGTACW 2 cut(s) 668, 1983
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 6 cut(s) 596, 839, 1020, 1121, 1504, 1825
Tru1I TTAA 8 cut(s) 308, 677, 1044, 1050, 1286, 1325, 1422, 1904
Tru9I TTAA 8 cut(s) 308, 677, 1044, 1050, 1286, 1325, 1422, 1904
TscAI CASTG 2 cut(s) 771, 2063
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 7 cut(s) 94, 97, 605, 1004, 1535, 1552, 1612
Tsp45I GTSAC 1 cut(s) 502
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 2 cut(s) 771, 2063
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 5 cut(s) 4, 1171, 1436, 1594, 1755
XceI RCATGY 4 cut(s) 40, 184, 662, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 1683
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 1860
XspI CTAG 3 cut(s) 975, 1368, 1556
Zsp2I ATGCAT 2 cut(s) 155, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.