Prupe.7G110800_v2.0.a1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001542
Physical Location & Seq
Forward (+)
13810418 .. 13813159
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G110800.1

Sequence Viewer

Length: 2082 bp
ATGAATTTTCAAGCGGTGTGCCGTTTTGGCGGGCACATGCTCCAGGACGCCACCACCTTGGCGCAAACTATCCAAACCTACGCACGAACCAAGCAGCTAAACAGAGGCAAGGAGCTCCATGCTCAGCTTCTACGCACCGAGTACACCCCATGCATTTTTCTCGCCAACCACCTTCTCAACATGTACTCAAAATGTGGGGAGGTCGACTATGCTCTCAAAGTCTTCGACAAAATGCCTCAGAGAAACTTGGTTTCTTGGACAGCAATGATAACTGGGTTTTCTCAAAACAGGAGGTTCTCTGAGACTTTGAAAACTTTTTCCCAGATGAGAGATGCTGGAGAGAACCCAACCCAGTTTGCATTTGCAAGTGTCATCAGAGCTTGCGTGTTTCTTGGGACGATTGAGATTGGGAGGCAAATGCATTCTCTTGCTTTGAAATTGGGCTTGGCTTTTGAATTATTTGTGGGTAGTAATTTGGCGGATATGTATTGGAAGTTCAGGTTGATGGCTGATGCTTGCAAGGTTTTTGAGGAGATGCCGTGTAAGGACGCAGTATCGTGGACTTCAATGATTGATGGATATGCAAAGAATGGTGATTCAGAGGCAGCTTTACTAACTTATAAGAGGATGGTTAATGATGGGATTGTTATAGATCAACATGTTCTTTGTAGTGCGTTAAATGCTTGTTCTACATTGAAGGCTTGTAAGTTTGGAAAGTGCCTTCATTCAACTGTTCTGAAGTTAGGACTTCAAGTAGAGGTTTCTGTGGGCAATGTTCTGACTGATATGTACTCGAAAGCAGGAGATATGGAGAGTGCTTCAAATGTGTTTTGGATTGACTCTGATGGTAGAAGTATTGTTTCTTGTACCTCTCTGATCAATGGTTTTGTTGAGATGGATGAAATTGACAAGGCTTTTAGTTTGTTTGTTGACTTACAGAGGCAGGGAGTTGAGCCTAATGAGTTCACTTTCTCTAGCTTGATCAAGTCTTGTGCCAACCAAGCTGCACCTGATCAAGGAATCCAGCTTCATGCTCAGGTGGTTAAGGTTAATTTCGATAGAGACCCTTTTGTTTATAGTGTTCTTGTTGATATGTATGGAAAGTGTGGGTTGCTTGATCATTCAATCCAAGTGTTTGACGAGATAGAAAACCCAACTGAAGTTGCATGGAATTCATTGTTAAGTGTGTTTGCTCTTCATGGCTTAGGAAAAGCAGCCTTGGAAACATTTACTAAAATGGTAAACAGAGGAGTGAAACCAAATGCAATAACATTTGTCAGTCTTTTAACAGGGTGTAGCCATGCTGGATTGGTAAAGGAAGGTTTAAATTACTTTCATTCTATGGAGAAGAGATATGGCATAGTGCCTAGAGAAGAACATTACTCTTGTGTTATTGATTTACTTGGTCGGGCTGGAAGGCTTAACGAGGCTGAAGAGTTCATAAATAACATGCCAATCCAACCAAATGCTTTTGGGTGGTGCTCTTTTCTTGGGGCTTGCAGGATTCATGGTGATCAGGAGAGGGGCAAACTTGCAGCGGAAAAACTGATGCAGCTTGAACCTGAGAATATTGGAGCACGTGTTTTGCTTTCGAATATATATGCAAAGGAACAGCAGTGGGAAGATGTGAGGAGTGTGAGGAAGAAGATGAGAGATGGCCGCATGAAGAAATTGCCTGGCTATAGTTGGGTTGATGTTGGAAACAAAACCCATACTTTCGGAGCAGAAGATTGGTCTCATCCTCTGATGAAAGAAATTTATGAAAAACTTGATACTCTTCTTGATCAGATAAAGGACGCTGGATATGTTCCACAAACAGATTCTATTCCACATGAAATGGACGAAAGCTCGAAGGAAAAGCTTCTTCACCATCACAGTGAGAGGATAGCAATTGCATTTGCACTAATAAGTATGCCAGCCGGGAAGCCAATCATTGTGAAGAAAAATTTAAGGGTGTGCTTGGATTGCCATTCTGCAATCAAGTACATCTCCAAGGTGGCAGGAAGGAAGATTATTGTTCGGGATAATAATCGATTTCACCATTTTGCAGATGGGTTATGTTCATGTGGAGATTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

694

Amino Acids

77.88

Weight (kDa)

6.88

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AccI GTMKAC 1 cut(s) 204
AciI CCGC 5 cut(s) 14, 30, 479, 1538, 1660
AcoI YGGCCR 1 cut(s) 1657
AcsI RAATTY 4 cut(s) 4, 1171, 1755, 1945
AcuI CTGAAG 3 cut(s) 758, 1179, 1452
AcvI CACGTG 1 cut(s) 1580
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 5 cut(s) 143, 185, 791, 868, 1985
AfiI CCNNNNNNNGG 1 cut(s) 1016
AflIII ACRYGT 3 cut(s) 180, 658, 1579
AjnI CCWGG 2 cut(s) 42, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
Alw21I GWGCWC 3 cut(s) 117, 1484, 1579
Alw26I GTCTC 3 cut(s) 296, 1056, 1740
AoxI GGCC 1 cut(s) 1657
ApeKI GCWGC 6 cut(s) 94, 605, 1004, 1214, 1535, 1552
ApoI RAATTY 4 cut(s) 4, 1171, 1755, 1945
Asp700I GAANNNNTTC 1 cut(s) 1860
AspLEI GCGC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 1921
AsuHPI GGTGA 4 cut(s) 605, 1523, 1859, 2030
AsuII TTCGAA 1 cut(s) 1592
BaeGI GKGCMC 1 cut(s) 36
BanII GRGCYC 1 cut(s) 117
BbrPI CACGTG 1 cut(s) 1580
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 3 cut(s) 117, 1484, 1579
BbvI GCAGC 6 cut(s) 106, 617, 991, 1226, 1547, 1564
BccI CCATC 9 cut(s) 499, 569, 622, 632, 839, 889, 1649, 1878, 2045
BceAI ACGGC 2 cut(s) 6, 523
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 2 cut(s) 44, 1677
BclI TGATCA 6 cut(s) 876, 981, 1012, 1117, 1513, 1783
BcnI CCSGG 1 cut(s) 1921
BcoDI GTCTC 3 cut(s) 296, 1056, 1740
BfaI CTAG 2 cut(s) 975, 1368
BfmI CTRYAG 1 cut(s) 1681
BglI GCCNNNNNGGC 1 cut(s) 27
BisI GCNGC 7 cut(s) 95, 606, 1005, 1215, 1536, 1553, 1660
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 7 cut(s) 96, 607, 1006, 1216, 1537, 1554, 1661
Bme1390I CCNGG 3 cut(s) 44, 1677, 1921
BmrFI CCNGG 3 cut(s) 44, 1677, 1921
BmrI ACTGGG 2 cut(s) 282, 346
BmsI GCATC 4 cut(s) 322, 502, 525, 1539
BmuI ACTGGG 2 cut(s) 282, 346
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 2 cut(s) 26, 357
Bpu10I CCTNAGC 2 cut(s) 1035, 1204
Bpu1102I GCTNAGC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 1592
BpuMI CCSGG 1 cut(s) 1921
Bsa29I ATCGAT 1 cut(s) 2032
BsaAI YACGTR 1 cut(s) 1580
BsaBI GATNNNNATC 1 cut(s) 2028
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 2 cut(s) 1056, 1740
BsaJI CCNNGG 3 cut(s) 57, 1218, 1992
BsaXI ACNNNNNCTCC 4 cut(s) 524, 554, 1566, 1596
Bsc4I CCNNNNNNNGG 1 cut(s) 1016
Bse1I ACTGG 3 cut(s) 277, 352, 2081
Bse3DI GCAATG 2 cut(s) 270, 778
Bse8I GATNNNNATC 1 cut(s) 2028
BseBI CCWGG 2 cut(s) 44, 1677
BseCI ATCGAT 1 cut(s) 2032
BseDI CCNNGG 3 cut(s) 57, 1218, 1992
BseGI GGATG 3 cut(s) 633, 904, 1738
BseJI GATNNNNATC 1 cut(s) 2028
BseLI CCNNNNNNNGG 1 cut(s) 1016
BseMI GCAATG 2 cut(s) 270, 778
BseMII CTCAG 5 cut(s) 137, 251, 291, 1049, 1554
BseNI ACTGG 3 cut(s) 277, 352, 2081
BseRI GAGGAG 3 cut(s) 545, 1263, 1645
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 6 cut(s) 106, 617, 991, 1226, 1547, 1564
BsgI GTGCAG 1 cut(s) 990
BshFI GGCC 1 cut(s) 1659
BshVI ATCGAT 1 cut(s) 2032
BsiHKAI GWGCWC 3 cut(s) 117, 1484, 1579
BsiSI CCGG 1 cut(s) 1920
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 1016
BsmAI GTCTC 3 cut(s) 296, 1056, 1740
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 1659
Bso31I GGTCTC 2 cut(s) 1056, 1740
Bsp119I TTCGAA 1 cut(s) 1592
Bsp1286I GDGCHC 4 cut(s) 36, 117, 1484, 1579
Bsp143I GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
Bsp1720I GCTNAGC 1 cut(s) 123
BspACI CCGC 5 cut(s) 14, 30, 479, 1538, 1660
BspANI GGCC 1 cut(s) 1659
BspCNI CTCAG 5 cut(s) 136, 250, 292, 1048, 1555
BspDI ATCGAT 1 cut(s) 2032
BspQI GCTCTTC 1 cut(s) 1200
BspT104I TTCGAA 1 cut(s) 1592
BspTNI GGTCTC 2 cut(s) 1056, 1740
BsrDI GCAATG 2 cut(s) 270, 778
BsrI ACTGG 3 cut(s) 277, 352, 2081
BssECI CCNNGG 3 cut(s) 57, 1218, 1992
BssMI GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 3 cut(s) 57, 1218, 1992
Bst2UI CCWGG 2 cut(s) 44, 1677
Bst4CI ACNGT 2 cut(s) 733, 1877
Bst6I CTCTTC 4 cut(s) 1200, 1343, 1428, 1782
BstACI GRCGYC 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 1580
BstBI TTCGAA 1 cut(s) 1592
BstC8I GCNNGC 5 cut(s) 32, 382, 517, 1498, 1917
BstDEI CTNAG 6 cut(s) 123, 237, 300, 1035, 1204, 1563
BstENI CCTNNNNNAGG 1 cut(s) 1014
BstF5I GGATG 3 cut(s) 633, 904, 1738
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 7 cut(s) 655, 879, 984, 1015, 1120, 1516, 1786
BstMAI GTCTC 3 cut(s) 296, 1056, 1740
BstMBI GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
BstMWI GCNNNNNNNGC 4 cut(s) 27, 680, 1001, 1965
BstNI CCWGG 2 cut(s) 44, 1677
BstNSI RCATGY 4 cut(s) 40, 184, 662, 1453
BstSCI CCNGG 3 cut(s) 42, 1675, 1919
BstSFI CTRYAG 1 cut(s) 1681
BstSLI GKGCMC 1 cut(s) 36
BstV1I GCAGC 6 cut(s) 106, 617, 991, 1226, 1547, 1564
BstV2I GAAGAC 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 2032
BsuRI GGCC 1 cut(s) 1659
BsuTUI ATCGAT 1 cut(s) 2032
BtsCI GGATG 3 cut(s) 633, 904, 1738
BtsI GCAGTG 1 cut(s) 1622
BtsIMutI CAGTG 2 cut(s) 1622, 1882
Cac8I GCNNGC 5 cut(s) 32, 382, 517, 1498, 1917
CfoI GCGC 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 2032
CseI GACGC 3 cut(s) 56, 557, 1805
Csp6I GTAC 5 cut(s) 142, 184, 790, 867, 1984
CviQI GTAC 5 cut(s) 142, 184, 790, 867, 1984
DdeI CTNAG 6 cut(s) 123, 237, 300, 1035, 1204, 1563
DpnI GATC 7 cut(s) 654, 878, 983, 1014, 1119, 1515, 1785
DpnII GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
DraI TTTAAA 1 cut(s) 1326
EaeI YGGCCR 1 cut(s) 1657
Eam1104I CTCTTC 4 cut(s) 1200, 1343, 1428, 1782
EarI CTCTTC 4 cut(s) 1200, 1343, 1428, 1782
EciI GGCGGA 1 cut(s) 494
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 57, 1218, 1992
Eco24I GRGCYC 1 cut(s) 117
Eco31I GGTCTC 2 cut(s) 1056, 1740
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 3 cut(s) 758, 1179, 1452
Eco72I CACGTG 1 cut(s) 1580
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 1014
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 2 cut(s) 42, 1675
EcoT14I CCWWGG 3 cut(s) 57, 1218, 1992
EcoT22I ATGCAT 2 cut(s) 155, 423
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 3 cut(s) 57, 1218, 1992
FaqI GGGAC 1 cut(s) 409
FauI CCCGC 1 cut(s) 23
FbaI TGATCA 6 cut(s) 876, 981, 1012, 1117, 1513, 1783
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 7 cut(s) 95, 606, 1005, 1215, 1536, 1553, 1660
FokI GGATG 3 cut(s) 640, 911, 1725
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 7 cut(s) 95, 606, 1005, 1215, 1536, 1553, 1660
FspBI CTAG 2 cut(s) 975, 1368
GlaI GCGC 1 cut(s) 63
GluI GCNGC 7 cut(s) 95, 606, 1005, 1215, 1536, 1553, 1660
GsuI CTGGAG 2 cut(s) 26, 357
HaeIII GGCC 1 cut(s) 1659
HapII CCGG 1 cut(s) 1920
HgaI GACGC 3 cut(s) 56, 557, 1805
HhaI GCGC 1 cut(s) 64
Hin1I GRCGYC 1 cut(s) 48
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 2 cut(s) 205, 931
HindII GTYRAC 2 cut(s) 205, 931
HindIII AAGCTT 1 cut(s) 1859
HinfI GANTC 5 cut(s) 596, 839, 1020, 1504, 1820
HpaII CCGG 1 cut(s) 1920
HphI GGTGA 4 cut(s) 605, 1523, 1859, 2030
Hpy166II GTNNAC 6 cut(s) 144, 205, 561, 931, 966, 1243
Hpy188III TCNNGA 3 cut(s) 1517, 1781, 2021
Hpy8I GTNNAC 6 cut(s) 144, 205, 561, 931, 966, 1243
HpyAV CCTTC 7 cut(s) 182, 691, 731, 1313, 1410, 1846, 1998
HpyCH4III ACNGT 2 cut(s) 733, 1877
HpyCH4IV ACGT 1 cut(s) 1579
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 680, 1001, 1965
HpyF3I CTNAG 6 cut(s) 123, 237, 300, 1035, 1204, 1563
HpySE526I ACGT 1 cut(s) 1579
Hsp92I GRCGYC 1 cut(s) 48
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 6 cut(s) 876, 981, 1012, 1117, 1513, 1783
Kzo9I GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
LguI GCTCTTC 1 cut(s) 1200
LmnI GCTCC 5 cut(s) 45, 112, 120, 1574, 1721
Lsp1109I GCAGC 6 cut(s) 106, 617, 991, 1226, 1547, 1564
LweI GCATC 4 cut(s) 322, 502, 525, 1539
MaeI CTAG 2 cut(s) 975, 1368
MaeII ACGT 1 cut(s) 1579
MalI GATC 7 cut(s) 654, 878, 983, 1014, 1119, 1515, 1785
MboI GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
MfeI CAATTG 1 cut(s) 1890
MhlI GDGCHC 4 cut(s) 36, 117, 1484, 1579
MlyI GAGTC 1 cut(s) 833
MmeI TCCRAC 2 cut(s) 1483, 1678
Mph1103I ATGCAT 2 cut(s) 155, 423
MroXI GAANNNNTTC 1 cut(s) 1860
MseI TTAA 9 cut(s) 633, 677, 1044, 1050, 1181, 1286, 1325, 1422, 1949
MslI CAYNNNNRTG 1 cut(s) 1875
MspA1I CMGCKG 1 cut(s) 1538
MspI CCGG 1 cut(s) 1920
MspR9I CCNGG 3 cut(s) 44, 1677, 1921
MunI CAATTG 1 cut(s) 1890
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 2 cut(s) 44, 1677
MwoI GCNNNNNNNGC 4 cut(s) 27, 680, 1001, 1965
NciI CCSGG 1 cut(s) 1921
NdeII GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
NsiI ATGCAT 2 cut(s) 155, 423
NspI RCATGY 4 cut(s) 40, 184, 662, 1453
NspV TTCGAA 1 cut(s) 1592
PciI ACATGT 2 cut(s) 180, 658
PciSI GCTCTTC 1 cut(s) 1200
PctI GAATGC 1 cut(s) 421
PdmI GAANNNNTTC 1 cut(s) 1860
PfeI GAWTC 4 cut(s) 596, 1020, 1504, 1820
PfoI TCCNGGA 1 cut(s) 42
PkrI GCNGC 7 cut(s) 96, 607, 1006, 1216, 1537, 1554, 1661
PleI GAGTC 1 cut(s) 833
PmaCI CACGTG 1 cut(s) 1580
PmlI CACGTG 1 cut(s) 1580
PpsI GAGTC 1 cut(s) 833
Ppu21I YACGTR 1 cut(s) 1580
PscI ACATGT 2 cut(s) 180, 658
PsiI TTATAA 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 42, 1675
PspCI CACGTG 1 cut(s) 1580
PspGI CCWGG 2 cut(s) 42, 1675
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
RsaI GTAC 5 cut(s) 143, 185, 791, 868, 1985
RsaNI GTAC 5 cut(s) 142, 184, 790, 867, 1984
RseI CAYNNNNRTG 1 cut(s) 1875
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 1200
SaqAI TTAA 9 cut(s) 633, 677, 1044, 1050, 1181, 1286, 1325, 1422, 1949
SatI GCNGC 7 cut(s) 95, 606, 1005, 1215, 1536, 1553, 1660
Sau3AI GATC 7 cut(s) 652, 876, 981, 1012, 1117, 1513, 1783
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 3 cut(s) 44, 1677, 1921
SduI GDGCHC 4 cut(s) 36, 117, 1484, 1579
SfaNI GCATC 4 cut(s) 322, 502, 525, 1539
SfcI CTRYAG 1 cut(s) 1681
SfuI TTCGAA 1 cut(s) 1592
SmiMI CAYNNNNRTG 1 cut(s) 1875
SsiI CCGC 5 cut(s) 14, 30, 479, 1538, 1660
SspI AATATT 1 cut(s) 1570
SspMI CTAG 2 cut(s) 975, 1368
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 3 cut(s) 42, 1675, 1919
StyI CCWWGG 3 cut(s) 57, 1218, 1992
TaaI ACNGT 2 cut(s) 733, 1877
TaiI ACGT 1 cut(s) 1582
TaqI TCGA 7 cut(s) 204, 225, 794, 1056, 1592, 1850, 2032
TatI WGTACW 4 cut(s) 141, 183, 789, 1983
TauI GCSGC 1 cut(s) 1662
TfiI GAWTC 4 cut(s) 596, 1020, 1504, 1820
Tru1I TTAA 9 cut(s) 633, 677, 1044, 1050, 1181, 1286, 1325, 1422, 1949
Tru9I TTAA 9 cut(s) 633, 677, 1044, 1050, 1181, 1286, 1325, 1422, 1949
TscAI CASTG 2 cut(s) 1622, 1882
TseI GCWGC 6 cut(s) 94, 605, 1004, 1214, 1535, 1552
TspRI CASTG 2 cut(s) 1622, 1882
XagI CCTNNNNNAGG 1 cut(s) 1014
XapI RAATTY 4 cut(s) 4, 1171, 1755, 1945
XceI RCATGY 4 cut(s) 40, 184, 662, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 2048
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 1860
XspI CTAG 2 cut(s) 975, 1368
Zsp2I ATGCAT 2 cut(s) 155, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.