Rh3BG210400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001542
Physical Location & Seq
Reverse (-)
18943888 .. 18945342
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG210400.1

Sequence Viewer

Length: 1455 bp
ATGGTTAGTGATGGGATTGGTATAGATAAACATGTTGTTTCTAGTGCATTGAGTGCTTGTTCTGCACTGGAGGCTTGTCAGTTTGGAAAGTGTGTCCATTCGACAGGTGTGAAGTTGGGATTGGAAGTAGAGGTTGCTGTGGGAAATGCTCTGGTTGATGTGTACTCGAAATCAGGTGATATGGAGAGTGCTTTAAATGTGTTTCGGGTTGACCCTGAGTGCAGAAATATTGTGTCGTATTCCTCTCTGATCAATGGTTATGTTGTGATGGATAAAATCGAGAAGGCTTTTAGTGTGTTTGTTGACTTACAGAGAAGGGGAGCTGAGCCTAATCAGTTCACTTTCTCTAGCTTGATCAAGGCTTGTGCCAACCAAGCTGCACTTGAACAAGGGATCCAACTTCATGCTCAGGTGGTCAAGTTTAATTTTGACAGAGATGATTTTGTGTCTTCTGTTCTTGTTGATATGTATGGGAGATGTGGGTTACTTGATCATTCAATACAAGTTTTTGACGAGGTACCCACCCCAACTGAAGTTGCGTGGAATTCATTGCTAAGTGTATTTGCAGTTCACGGCTTAGGAAATGACGCCTTGAAAACTTTTAGTAGAATGGTCCAGGCAGGAGTGAAACCAAATGCAATAACATTTATCAGTCTTTTAACAGGCTGTAGCCATTCTGGATTGGTAGAGGAAGGTTTAAAGTACTTTTACTCTCTGGAGAAAACATATGGCATAGTACCTAGAGCTGGACATTACTCTTGCGTTATTGATTTACTTGGCAGGGCTGGAAGGCTTGAAGAGGCCGAAGAATTCATTAACAGCATGCCAATGCAGCCAAATGCTTTTGGATGGTGCTCTTTTCTTGGTGCTTGCACAATTCATGGTGATAAAGAGAGGGGCAAACTGGCAGCGGAGAAATTGATACAGCTTGAACCTGAGAATAGTGGAGCACATGTGTTGCTTTCAACTATATATGCAAAGGAGCAGAAATGGGAAGATTTCAGGGGTGTGAGGAGGATAATGAGGGACAGCAGCATGAAGAAATTGCCCGGTTATAGTTGGATTGACATTGGAAACAAAACACACACATTTGGAGCCGAAGATTGGTCTCATCCTCAGAAGAAAGAAATATATGAGAAGCTTGATAGTCTTCTTGATCAGATAAAGAAAGCTGGATATGTTCCAAATACAGATCTTGTTCTTCGTGATATGGACGACAATTCAAAGGTAGAGCTTCTTCACCATCATAGTGAGAGGATAGCGATTGCATTTGCAATGATAAGTATGCCTGCTGGGAAGCCAATCATTGTGAAGAAAAATATAAGGGTGTGCTTGGATTGCCATTCTGCAATCAAGTACATCTCCTTGGTTGTTGGAAGAAAGATTATTGTTAGGGATAATACTCGGTTTCACCATTTCGCAGATGGGTTGTGTTCATGTGGAGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

484

Amino Acids

53.67

Weight (kDa)

6.29

Isoelectric Point (pI)

28.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 124 3.7e-14 PPR repeat family
PPR_3 PF13812 76 - 124 2.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 176 - 224 3.4e-11 PPR repeat family
E_motif PF20431 294 - 356 7.3e-18 E motif
Eplus_motif PF20430 361 - 388 9.8e-10 E+ motif
DYW_deaminase PF14432 392 - 484 5.1e-39 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 517
AciI CCGC 1 cut(s) 911
AclWI GGATC 2 cut(s) 388, 401
AcsI RAATTY 2 cut(s) 544, 809
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 588
AfaI GTAC 5 cut(s) 164, 519, 704, 738, 1358
AfiI CCNNNNNNNGG 2 cut(s) 746, 1104
AflIII ACRYGT 2 cut(s) 31, 952
AgsI TTSAA 7 cut(s) 386, 498, 595, 797, 932, 966, 1224
AjnI CCWGG 1 cut(s) 615
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
AluBI AGCT 8 cut(s) 323, 351, 377, 746, 928, 1141, 1172, 1234
AluI AGCT 8 cut(s) 323, 351, 377, 746, 928, 1141, 1172, 1234
Alw21I GWGCWC 2 cut(s) 857, 952
Alw26I GTCTC 1 cut(s) 1113
AlwI GGATC 2 cut(s) 388, 401
AoxI GGCC 1 cut(s) 801
ApeKI GCWGC 4 cut(s) 377, 832, 908, 1032
ApoI RAATTY 2 cut(s) 544, 809
Asp718I GGTACC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 613
AsuC2I CCSGG 1 cut(s) 1050
AsuHPI GGTGA 4 cut(s) 188, 896, 1232, 1403
AvaII GGWCC 1 cut(s) 613
BamHI GGATCC 1 cut(s) 393
BanI GGYRCC 1 cut(s) 517
BarI GAAGNNNNNNTAC 2 cut(s) 1221, 1253
BbsI GAAGAC 2 cut(s) 441, 1142
Bbv12I GWGCWC 2 cut(s) 857, 952
BbvI GCAGC 4 cut(s) 364, 844, 920, 1044
BccI CCATC 5 cut(s) 5, 262, 843, 1251, 1418
BceAI ACGGC 1 cut(s) 589
BciT130I CCWGG 1 cut(s) 617
BclI TGATCA 4 cut(s) 249, 354, 490, 1156
BcnI CCSGG 1 cut(s) 1050
BcoDI GTCTC 1 cut(s) 1113
BfaI CTAG 3 cut(s) 42, 348, 741
BfmI CTRYAG 1 cut(s) 667
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 4 cut(s) 378, 833, 909, 1033
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 4 cut(s) 379, 834, 910, 1034
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 2 cut(s) 617, 1050
Bme18I GGWCC 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 613
BmiI GGNNCC 3 cut(s) 395, 519, 1096
BmrFI CCNGG 2 cut(s) 617, 1050
BpiI GAAGAC 2 cut(s) 441, 1142
BpmI CTGGAG 2 cut(s) 89, 737
Bpu10I CCTNAGC 2 cut(s) 408, 577
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 588
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 1 cut(s) 1365
BsaXI ACNNNNNCTCC 2 cut(s) 939, 969
Bsc4I CCNNNNNNNGG 2 cut(s) 746, 1104
Bse1I ACTGG 3 cut(s) 72, 909, 1454
Bse3DI GCAATG 2 cut(s) 548, 1281
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 1365
BseGI GGATG 2 cut(s) 854, 1111
BseLI CCNNNNNNNGG 2 cut(s) 746, 1104
BseMI GCAATG 2 cut(s) 548, 1281
BseMII CTCAG 5 cut(s) 207, 315, 422, 927, 1130
BseNI ACTGG 3 cut(s) 72, 909, 1454
BseRI GAGGAG 1 cut(s) 1027
BseXI GCAGC 4 cut(s) 364, 844, 920, 1044
BseYI CCCAGC 1 cut(s) 1292
BsgI GTGCAG 3 cut(s) 48, 241, 363
BshFI GGCC 1 cut(s) 803
BshNI GGYRCC 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 857, 952
BsiSI CCGG 1 cut(s) 1050
BslFI GGGAC 1 cut(s) 1040
BslI CCNNNNNNNGG 2 cut(s) 746, 1104
BsmAI GTCTC 1 cut(s) 1113
BsmFI GGGAC 1 cut(s) 1040
BsnI GGCC 1 cut(s) 803
Bso31I GGTCTC 1 cut(s) 1113
Bsp1286I GDGCHC 2 cut(s) 857, 952
Bsp143I GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 1 cut(s) 911
BspANI GGCC 1 cut(s) 803
BspCNI CTCAG 5 cut(s) 208, 316, 421, 928, 1129
BspLI GGNNCC 3 cut(s) 395, 519, 1096
BspPI GGATC 2 cut(s) 388, 401
BspT107I GGYRCC 1 cut(s) 517
BspTNI GGTCTC 1 cut(s) 1113
BsrDI GCAATG 2 cut(s) 548, 1281
BsrI ACTGG 3 cut(s) 72, 909, 1454
BssECI CCNNGG 1 cut(s) 1365
BssMI GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 1 cut(s) 1365
Bst2UI CCWGG 1 cut(s) 617
Bst6I CTCTTC 1 cut(s) 792
BstACI GRCGYC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 824, 871, 1290
BstDEI CTNAG 7 cut(s) 216, 324, 408, 554, 577, 936, 1116
BstF5I GGATG 2 cut(s) 854, 1111
BstKTI GATC 6 cut(s) 252, 357, 396, 493, 1159, 1195
BstMAI GTCTC 1 cut(s) 1113
BstMBI GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
BstMWI GCNNNNNNNGC 6 cut(s) 53, 62, 71, 374, 832, 1338
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 3 cut(s) 35, 826, 956
BstSCI CCNGG 2 cut(s) 615, 1048
BstSFI CTRYAG 1 cut(s) 667
BstV1I GCAGC 4 cut(s) 364, 844, 920, 1044
BstV2I GAAGAC 2 cut(s) 441, 1142
BstX2I RGATCY 2 cut(s) 393, 1192
BstYI RGATCY 2 cut(s) 393, 1192
BsuRI GGCC 1 cut(s) 803
BtsCI GGATG 2 cut(s) 854, 1111
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 3 cut(s) 824, 871, 1290
Cfr13I GGNCC 1 cut(s) 613
CseI GACGC 1 cut(s) 596
Csp6I GTAC 5 cut(s) 163, 518, 703, 737, 1357
CviAII CATG 7 cut(s) 32, 404, 823, 881, 953, 1036, 1437
CviQI GTAC 5 cut(s) 163, 518, 703, 737, 1357
DdeI CTNAG 7 cut(s) 216, 324, 408, 554, 577, 936, 1116
DpnI GATC 6 cut(s) 251, 356, 395, 492, 1158, 1194
DpnII GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
DraI TTTAAA 2 cut(s) 195, 699
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 1 cut(s) 1365
Eco31I GGTCTC 1 cut(s) 1113
Eco47I GGWCC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 552
EcoRI GAATTC 2 cut(s) 544, 809
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 1 cut(s) 1365
ErhI CCWWGG 1 cut(s) 1365
FaeI CATG 7 cut(s) 35, 407, 826, 884, 956, 1039, 1440
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 1 cut(s) 1040
FatI CATG 7 cut(s) 31, 403, 822, 880, 952, 1035, 1436
FauNDI CATATG 1 cut(s) 727
FbaI TGATCA 4 cut(s) 249, 354, 490, 1156
Fnu4HI GCNGC 4 cut(s) 378, 833, 909, 1033
FokI GGATG 2 cut(s) 861, 1098
Fsp4HI GCNGC 4 cut(s) 378, 833, 909, 1033
FspBI CTAG 3 cut(s) 42, 348, 741
GluI GCNGC 4 cut(s) 378, 833, 909, 1033
GsaI CCCAGC 1 cut(s) 1296
GsuI CTGGAG 2 cut(s) 89, 737
HaeIII GGCC 1 cut(s) 803
HapII CCGG 1 cut(s) 1050
HgaI GACGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 7 cut(s) 35, 407, 826, 884, 956, 1039, 1440
HincII GTYRAC 2 cut(s) 211, 304
HindII GTYRAC 2 cut(s) 211, 304
HindIII AAGCTT 1 cut(s) 1139
HpaII CCGG 1 cut(s) 1050
HphI GGTGA 4 cut(s) 188, 896, 1232, 1403
Hpy166II GTNNAC 5 cut(s) 163, 211, 304, 339, 571
Hpy188I TCNGA 3 cut(s) 249, 1119, 1161
Hpy188III TCNNGA 5 cut(s) 280, 678, 716, 1154, 1205
Hpy8I GTNNAC 5 cut(s) 163, 211, 304, 339, 571
HpyAV CCTTC 4 cut(s) 277, 309, 686, 783
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 62, 71, 374, 832, 1338
HpyF3I CTNAG 7 cut(s) 216, 324, 408, 554, 577, 936, 1116
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 7 cut(s) 35, 407, 826, 884, 956, 1039, 1440
KpnI GGTACC 1 cut(s) 521
Ksp22I TGATCA 4 cut(s) 249, 354, 490, 1156
Kzo9I GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
LmnI GCTCC 4 cut(s) 320, 947, 982, 1094
Lsp1109I GCAGC 4 cut(s) 364, 844, 920, 1044
MaeI CTAG 3 cut(s) 42, 348, 741
MaeIII GTNAC 1 cut(s) 483
MalI GATC 6 cut(s) 251, 356, 395, 492, 1158, 1194
MboI GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
MflI RGATCY 2 cut(s) 393, 1192
MhlI GDGCHC 2 cut(s) 857, 952
MluCI AATT 7 cut(s) 424, 544, 809, 876, 917, 1043, 1219
MmeI TCCRAC 3 cut(s) 421, 1040, 1354
MseI TTAA 5 cut(s) 194, 423, 659, 698, 816
MslI CAYNNNNRTG 2 cut(s) 827, 1248
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 1 cut(s) 1050
MspR9I CCNGG 2 cut(s) 617, 1050
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 6 cut(s) 53, 62, 71, 374, 832, 1338
NciI CCSGG 1 cut(s) 1050
NdeI CATATG 1 cut(s) 727
NdeII GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
NlaIII CATG 7 cut(s) 35, 407, 826, 884, 956, 1039, 1440
NlaIV GGNNCC 3 cut(s) 395, 519, 1096
NspI RCATGY 3 cut(s) 35, 826, 956
PaeI GCATGC 1 cut(s) 826
PciI ACATGT 2 cut(s) 31, 952
PkrI GCNGC 4 cut(s) 379, 834, 910, 1034
PscI ACATGT 2 cut(s) 31, 952
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 1292
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 395, 519, 1096
PspPI GGNCC 1 cut(s) 613
PsrI GAACNNNNNNTAC 2 cut(s) 552, 584
PsuI RGATCY 2 cut(s) 393, 1192
RsaI GTAC 5 cut(s) 164, 519, 704, 738, 1358
RsaNI GTAC 5 cut(s) 163, 518, 703, 737, 1357
RseI CAYNNNNRTG 2 cut(s) 827, 1248
SaqAI TTAA 5 cut(s) 194, 423, 659, 698, 816
SatI GCNGC 4 cut(s) 378, 833, 909, 1033
Sau3AI GATC 6 cut(s) 249, 354, 393, 490, 1156, 1192
Sau96I GGNCC 1 cut(s) 613
ScaI AGTACT 1 cut(s) 704
ScrFI CCNGG 2 cut(s) 617, 1050
SduI GDGCHC 2 cut(s) 857, 952
SfcI CTRYAG 1 cut(s) 667
SinI GGWCC 1 cut(s) 613
SmiMI CAYNNNNRTG 2 cut(s) 827, 1248
SphI GCATGC 1 cut(s) 826
Sse9I AATT 7 cut(s) 424, 544, 809, 876, 917, 1043, 1219
SsiI CCGC 1 cut(s) 911
SspI AATATT 1 cut(s) 229
SspMI CTAG 3 cut(s) 42, 348, 741
StyD4I CCNGG 2 cut(s) 615, 1048
StyI CCWWGG 1 cut(s) 1365
TaqI TCGA 3 cut(s) 101, 167, 279
TasI AATT 7 cut(s) 424, 544, 809, 876, 917, 1043, 1219
TatI WGTACW 3 cut(s) 162, 702, 1356
Tru1I TTAA 5 cut(s) 194, 423, 659, 698, 816
Tru9I TTAA 5 cut(s) 194, 423, 659, 698, 816
TscAI CASTG 1 cut(s) 72
TseI GCWGC 4 cut(s) 377, 832, 908, 1032
TspDTI ATGAA 6 cut(s) 392, 537, 802, 869, 1052, 1425
TspRI CASTG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 613
XapI RAATTY 2 cut(s) 544, 809
XceI RCATGY 3 cut(s) 35, 826, 956
XcmI CCANNNNNNNNNTGG 1 cut(s) 1421
XspI CTAG 3 cut(s) 42, 348, 741
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.