Rh3BG211900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001542
Physical Location & Seq
Reverse (-)
19110164 .. 19111977
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG211900.1

Sequence Viewer

Length: 1494 bp
ATGGTAACTGGGTTTTCTCAGAACTTGAGGTTCTCCGAGAGTTTCAAGGCCTTTTCGCAGATGAGAATTGCTGGGGAGAGCCCGACCCAGTTTGCATTTGCAAGTGTCATCAGAGCTTGTGTGGTTCTTGGGTCGGTTGAGATTGGGAGGCAGCTGCATTCTCTTGCTTTGAAATTGGGCTTGGCTTGTGAGTTGTTTGTGGGCAGTAACTTGGCGGATATGTATTCGAAATGTGGGTTCATGGTTGAGGCTTGTAAGTTTTTCGAGGAAATGCCGAGTAAGGATGCTGTGTCTTGGACTTCGATGATTGATGGGTATGCAAAAAGTGGGGATTTTGAGGCAGCTTTACTGAGTTATCAGAGGATGATCAATGATGGGATTGGTGTAGATAAATATGTTGTTTCTAGTGCATTGAGTGCTTGTTCTGCACTGAAGGCTTGTCAGTTTGGAAAGTGTGTTCATTCGACGGTTGTGAAGTTAGGATTGGAAGTAGAGGTTGCTGTGGGAAATGCTCTGGTTGATATGTACTCGAAATCAGGAGATATGGAGAGTGCTTTAAATGTGTTTCATGTTCACCCTGAGTGTAGAAATATTGTGTCAGGGGGGGGGGGAGTTGAGCCTAATCAGTTCACATTCTCTAGCTTGGTCAAGGCCTGTGCCAACCAAGCTGCACTTGAACAAGGGATTCAACTTCATGCTCAGGTGATCAAGTTTAATTTTGATAGAGACAATTTTGTGTCTTCTGTTCTTGTTGATATGTATGGAAAATGTGGGTTACTTGATCACTCAATACAAGTGTTTGACGAGATAGCAAACCCGACTGAAGTTGCATGGAATTCGTTGCCAAGTGTATTTGCAGTTAATGGCTTAGGAAATGACGCCTTGAAAACTTTTAGTAGAATGGTCCGGGCAGGAGTGAAACCAAATGCAATAACATTTATCATCCTTTTAACAGGTTGTAGCCATTCTGGATCGGTAGAGGAAGGTTTAACGTACTTTTACTTTATGGAGAAAACATATGGCATGGAGCAGAAATGGGAAGATTTCAGGGGTGTGAGGAAGATAATGAGGGATGGCAGCATGAAGAAATTGCCCGGTTATAGTTGGGTTGACATTGGAAACAAAACACACACGTTTGGAGCCAAAGATTGGTCTCATCCTCAGAAGAAAAAAATATATGAGAAACTTGATAGTCTTCTTGATCAGATCAAGAAAGCTGGATATGTTCCAAATACAGATCTTGTTCTACATGATATGGACGACAATTCAAAGGTAGAGCTTCTTCACCATCATAGTGAGAGGATAGCGATTGCATTTGCACTGGTAAGTATGCCTGGGAAGCCAATCATTGTGAAGAAAAATATAAGGGTGTGCTTGGATTGCCATTCTGCAATCAAGTACATCTCCTTGGTTGTTGGAAGAAAGATCATAGTTAGGGACAATACTCGGTTCCACCATTTCGCAGATGGGTTGTGTTCATGTGGAGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

497

Amino Acids

54.82

Weight (kDa)

7.21

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 94 - 141 1.6e-06 PPR repeat family
PPR PF01535 97 - 127 3.6e-07 PPR repeat
PPR_2 PF13041 273 - 321 6.6e-10 PPR repeat family
Eplus_motif PF20430 376 - 403 1.5e-08 E+ motif
DYW_deaminase PF14432 407 - 497 4.2e-36 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1149
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 979
AcsI RAATTY 1 cut(s) 835
AcuI CTGAAG 2 cut(s) 452, 843
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 3 cut(s) 527, 995, 1400
AfiI CCNNNNNNNGG 1 cut(s) 1149
AflIII ACRYGT 1 cut(s) 1131
AgsI TTSAA 6 cut(s) 46, 172, 677, 689, 886, 1269
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 4 cut(s) 164, 196, 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 7 cut(s) 116, 154, 344, 642, 668, 1217, 1279
AluI AGCT 7 cut(s) 116, 154, 344, 642, 668, 1217, 1279
Alw26I GTCTC 2 cut(s) 720, 1158
AlwI GGATC 1 cut(s) 979
AoxI GGCC 2 cut(s) 48, 651
ApeKI GCWGC 5 cut(s) 151, 154, 341, 668, 1077
ApoI RAATTY 1 cut(s) 835
AspS9I GGNCC 1 cut(s) 904
AsuC2I CCSGG 2 cut(s) 908, 1095
AsuHPI GGTGA 3 cut(s) 566, 715, 1277
AsuII TTCGAA 1 cut(s) 227
AvaII GGWCC 1 cut(s) 904
BanII GRGCYC 1 cut(s) 83
BarI GAAGNNNNNNTAC 2 cut(s) 1266, 1298
BbsI GAAGAC 2 cut(s) 732, 1187
BbvI GCAGC 5 cut(s) 141, 163, 353, 655, 1089
BccI CCATC 5 cut(s) 305, 368, 1067, 1296, 1460
BcgI CGANNNNNNTGC 2 cut(s) 819, 853
BciT130I CCWGG 1 cut(s) 1335
BclI TGATCA 4 cut(s) 366, 705, 781, 1201
BcnI CCSGG 2 cut(s) 908, 1095
BcoDI GTCTC 2 cut(s) 720, 1158
BfaI CTAG 3 cut(s) 405, 639, 1492
BglII AGATCT 1 cut(s) 1237
BisI GCNGC 5 cut(s) 152, 155, 342, 669, 1078
BlsI GCNGC 5 cut(s) 153, 156, 343, 670, 1079
Bme1390I CCNGG 3 cut(s) 908, 1095, 1335
Bme18I GGWCC 1 cut(s) 904
BmgT120I GGNCC 1 cut(s) 904
BmiI GGNNCC 2 cut(s) 1141, 1451
BmrFI CCNGG 3 cut(s) 908, 1095, 1335
BmrI ACTGGG 2 cut(s) 18, 82
BmsI GCATC 1 cut(s) 274
BmuI ACTGGG 2 cut(s) 18, 82
BpiI GAAGAC 2 cut(s) 732, 1187
Bpu10I CCTNAGC 2 cut(s) 699, 868
Bpu14I TTCGAA 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 2 cut(s) 908, 1095
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 1158
BsaJI CCNNGG 2 cut(s) 1334, 1407
Bsc4I CCNNNNNNNGG 1 cut(s) 1149
Bse1I ACTGG 3 cut(s) 13, 88, 1326
BseBI CCWGG 1 cut(s) 1335
BseDI CCNNGG 2 cut(s) 1334, 1407
BseGI GGATG 5 cut(s) 289, 369, 942, 1078, 1156
BseLI CCNNNNNNNGG 1 cut(s) 1149
BseMII CTCAG 5 cut(s) 32, 341, 570, 713, 1175
BseNI ACTGG 3 cut(s) 13, 88, 1326
BseXI GCAGC 5 cut(s) 141, 163, 353, 655, 1089
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 2 cut(s) 411, 654
BshFI GGCC 2 cut(s) 50, 653
BsiSI CCGG 2 cut(s) 907, 1095
BslFI GGGAC 1 cut(s) 1451
BslI CCNNNNNNNGG 1 cut(s) 1149
BsmAI GTCTC 2 cut(s) 720, 1158
BsmFI GGGAC 1 cut(s) 1451
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 2 cut(s) 50, 653
Bso31I GGTCTC 1 cut(s) 1158
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 50, 653
BspCNI CTCAG 5 cut(s) 31, 342, 571, 712, 1174
BspLI GGNNCC 2 cut(s) 1141, 1451
BspPI GGATC 1 cut(s) 979
BspT104I TTCGAA 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 1158
BsrI ACTGG 3 cut(s) 13, 88, 1326
BssECI CCNNGG 2 cut(s) 1334, 1407
BssMI GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 1 cut(s) 1407
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 1 cut(s) 469
BstACI GRCGYC 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 227
BstDEI CTNAG 6 cut(s) 18, 350, 579, 699, 868, 1161
BstF5I GGATG 5 cut(s) 289, 369, 942, 1078, 1156
BstKTI GATC 8 cut(s) 369, 708, 784, 974, 1204, 1209, 1240, 1428
BstMAI GTCTC 2 cut(s) 720, 1158
BstMBI GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
BstMWI GCNNNNNNNGC 6 cut(s) 416, 425, 434, 665, 1339, 1380
BstNI CCWGG 1 cut(s) 1335
BstSCI CCNGG 3 cut(s) 906, 1093, 1333
BstV1I GCAGC 5 cut(s) 141, 163, 353, 655, 1089
BstV2I GAAGAC 2 cut(s) 732, 1187
BstX2I RGATCY 1 cut(s) 1237
BstYI RGATCY 1 cut(s) 1237
BsuRI GGCC 2 cut(s) 50, 653
BtsCI GGATG 5 cut(s) 289, 369, 942, 1078, 1156
BtsIMutI CAGTG 2 cut(s) 428, 1319
Cfr13I GGNCC 1 cut(s) 904
CseI GACGC 1 cut(s) 887
Csp6I GTAC 3 cut(s) 526, 994, 1399
CviAII CATG 8 cut(s) 241, 569, 695, 831, 1024, 1081, 1250, 1479
CviQI GTAC 3 cut(s) 526, 994, 1399
DdeI CTNAG 6 cut(s) 18, 350, 579, 699, 868, 1161
DpnI GATC 8 cut(s) 368, 707, 783, 973, 1203, 1208, 1239, 1427
DpnII GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
DraI TTTAAA 1 cut(s) 558
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 1407
Eco147I AGGCCT 2 cut(s) 50, 653
Eco24I GRGCYC 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 1158
Eco47I GGWCC 1 cut(s) 904
Eco57I CTGAAG 2 cut(s) 452, 843
EcoRI GAATTC 1 cut(s) 835
EcoRII CCWGG 1 cut(s) 1333
EcoT14I CCWWGG 1 cut(s) 1407
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 1407
FaeI CATG 8 cut(s) 244, 572, 698, 834, 1027, 1084, 1253, 1482
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 1451
FatI CATG 8 cut(s) 240, 568, 694, 830, 1023, 1080, 1249, 1478
FauNDI CATATG 1 cut(s) 1018
FbaI TGATCA 4 cut(s) 366, 705, 781, 1201
Fnu4HI GCNGC 5 cut(s) 152, 155, 342, 669, 1078
FokI GGATG 5 cut(s) 296, 376, 929, 1085, 1143
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 5 cut(s) 152, 155, 342, 669, 1078
FspBI CTAG 3 cut(s) 405, 639, 1492
GluI GCNGC 5 cut(s) 152, 155, 342, 669, 1078
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 2 cut(s) 50, 653
HapII CCGG 2 cut(s) 907, 1095
HgaI GACGC 1 cut(s) 887
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 8 cut(s) 244, 572, 698, 834, 1027, 1084, 1253, 1482
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HinfI GANTC 1 cut(s) 685
HpaII CCGG 2 cut(s) 907, 1095
HphI GGTGA 3 cut(s) 566, 715, 1277
Hpy166II GTNNAC 3 cut(s) 574, 630, 1111
Hpy188I TCNGA 6 cut(s) 21, 37, 113, 360, 1164, 1206
Hpy188III TCNNGA 4 cut(s) 537, 969, 1199, 1210
Hpy8I GTNNAC 3 cut(s) 574, 630, 1111
Hpy99I CGWCG 1 cut(s) 469
HpyAV CCTTC 2 cut(s) 427, 977
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 2 cut(s) 992, 1133
HpyF10VI GCNNNNNNNGC 6 cut(s) 416, 425, 434, 665, 1339, 1380
HpyF3I CTNAG 6 cut(s) 18, 350, 579, 699, 868, 1161
HpySE526I ACGT 2 cut(s) 992, 1133
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 8 cut(s) 244, 572, 698, 834, 1027, 1084, 1253, 1482
Ksp22I TGATCA 4 cut(s) 366, 705, 781, 1201
Kzo9I GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
LmnI GCTCC 2 cut(s) 1027, 1139
Lsp1109I GCAGC 5 cut(s) 141, 163, 353, 655, 1089
LweI GCATC 1 cut(s) 274
MaeI CTAG 3 cut(s) 405, 639, 1492
MaeII ACGT 2 cut(s) 992, 1133
MaeIII GTNAC 3 cut(s) 4, 206, 774
MalI GATC 8 cut(s) 368, 707, 783, 973, 1203, 1208, 1239, 1427
MboI GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
MboII GAAGA 9 cut(s) 732, 1052, 1072, 1096, 1177, 1187, 1274, 1366, 1431
MflI RGATCY 1 cut(s) 1237
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 7 cut(s) 66, 173, 715, 730, 835, 1088, 1264
MmeI TCCRAC 1 cut(s) 1396
MseI TTAA 5 cut(s) 557, 714, 861, 950, 989
MslI CAYNNNNRTG 1 cut(s) 1293
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 2 cut(s) 907, 1095
MspR9I CCNGG 3 cut(s) 908, 1095, 1335
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 1 cut(s) 1335
MwoI GCNNNNNNNGC 6 cut(s) 416, 425, 434, 665, 1339, 1380
NciI CCSGG 2 cut(s) 908, 1095
NdeI CATATG 1 cut(s) 1018
NdeII GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
NlaIII CATG 8 cut(s) 244, 572, 698, 834, 1027, 1084, 1253, 1482
NlaIV GGNNCC 2 cut(s) 1141, 1451
NmeAIII GCCGAG 1 cut(s) 300
NspV TTCGAA 1 cut(s) 227
PceI AGGCCT 2 cut(s) 50, 653
PctI GAATGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 1149
PkrI GCNGC 5 cut(s) 153, 156, 343, 670, 1079
Psp6I CCWGG 1 cut(s) 1333
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 2 cut(s) 1141, 1451
PspPI GGNCC 1 cut(s) 904
PsuI RGATCY 1 cut(s) 1237
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 3 cut(s) 527, 995, 1400
RsaNI GTAC 3 cut(s) 526, 994, 1399
RseI CAYNNNNRTG 1 cut(s) 1293
SaqAI TTAA 5 cut(s) 557, 714, 861, 950, 989
SatI GCNGC 5 cut(s) 152, 155, 342, 669, 1078
Sau3AI GATC 8 cut(s) 366, 705, 781, 971, 1201, 1206, 1237, 1425
Sau96I GGNCC 1 cut(s) 904
ScrFI CCNGG 3 cut(s) 908, 1095, 1335
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 1 cut(s) 274
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 1293
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 7 cut(s) 66, 173, 715, 730, 835, 1088, 1264
SseBI AGGCCT 2 cut(s) 50, 653
SsiI CCGC 1 cut(s) 215
SspI AATATT 1 cut(s) 592
SspMI CTAG 3 cut(s) 405, 639, 1492
StuI AGGCCT 2 cut(s) 50, 653
StyD4I CCNGG 3 cut(s) 906, 1093, 1333
StyI CCWWGG 1 cut(s) 1407
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 2 cut(s) 995, 1136
TaqI TCGA 5 cut(s) 227, 264, 302, 464, 530
TasI AATT 7 cut(s) 66, 173, 715, 730, 835, 1088, 1264
TatI WGTACW 2 cut(s) 525, 1398
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 5 cut(s) 557, 714, 861, 950, 989
Tru9I TTAA 5 cut(s) 557, 714, 861, 950, 989
TscAI CASTG 2 cut(s) 435, 1326
TseI GCWGC 5 cut(s) 151, 154, 341, 668, 1077
TspDTI ATGAA 6 cut(s) 229, 449, 557, 683, 1097, 1467
TspRI CASTG 2 cut(s) 435, 1326
Van91I CCANNNNNTGG 1 cut(s) 1149
VpaK11BI GGWCC 1 cut(s) 904
XapI RAATTY 1 cut(s) 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1463
XspI CTAG 3 cut(s) 405, 639, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.