Rmu_sc0000908.1_g000027

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000908.1
OG0001542
Physical Location & Seq
Forward (+)
131454 .. 133267
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000908.1_g000027.1.cds

Sequence Viewer

Length: 1317 bp
atgcctcaccgaaacttggtttcctggactgcaatggtcactgggttttctcagaacttgaggttctcggagagcttcaagcccttttcccagatgagaagcgctggggagagcccgacccagtttgcatttgcaagtgtcatcagagcttgtgtggttcttggtagcttggcggatatgtattcgaaatgtgggttcatggttgaggcttgtaagtttttcgaggaaatgccgagtaaggatgctgtgtcgtggacttcgatgattgatgggtatgcaaagagtggggattttgaggcagctttactgagttatcagaggatgatcaatgatgggattggtgtagataaatatgttgtttctagagcattgagtgcttcttctgcactgaaggcttgtcagtttggaaagcgtgttcattcgacggttgtgaagttgggattggaagtagaggttgctgtgggaaatgctctggttgatatgtactcgaaatcaggtgatatggtgagtgctttaaatgtgtttcgggttgaccctgagtgcagaaatattgtgtcgtattcctctctgatcaatggttatgttgagatggatgaaatcgagaaggcttttagtgtgtttgttgacttacagagaaggggagctgagcctaatcagctcactttctctagcttgatcaaggcttgtgccaaccaagctgcatttgaacaagggatccaacattccaacttcatggtcagccaaatgcttttgggtggtgctcttttcttggtgcttgcaagattcatggagataaagactaaagagaggggcaaactggcagcggagaaattgatacagcttgaacctgagaatagtggagcacatgtttttctttcaactttacatgcaaaggagcaaaaatgggaagatttcagaagtgtgaggaagatgatgagagacagcagcatgaaaaaattgcctggttatagttgggttgatgttggaaacaaaacacacgtgtttggagccgaagattggtctcatcctcagaagaaagaaatatatgagacacttgatagtcttcttgatcagatcaagaaagctggacatgttccaaatacagatcttgttctacatgatatggacgacaattcaaaggtagagcttcttcaccatcatagtgggaggattgcaattgcattcgcattgataagtatgcctgcagccgggaagccaatcattgtgaagacaaatataagggtgtgcttggattgccattctgcaatcaagtacgtttccttggttgtgtctccttggttgttggaagaaagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

438

Amino Acids

48.78

Weight (kDa)

7.2

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 173, 824
AclWI GGATC 2 cut(s) 709, 722
AcuI CTGAAG 1 cut(s) 410
AcvI CACGTG 1 cut(s) 1000
AfaI GTAC 2 cut(s) 485, 1274
AfeI AGCGCT 1 cut(s) 103
AfiI CCNNNNNNNGG 3 cut(s) 16, 1017, 1208
AflIII ACRYGT 4 cut(s) 865, 997, 999, 1090
AgsI TTSAA 5 cut(s) 79, 707, 845, 879, 1137
AjnI CCWGG 2 cut(s) 23, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AloI GAACNNNNNNTCC 4 cut(s) 399, 431, 699, 731
Alw21I GWGCWC 2 cut(s) 763, 865
Alw26I GTCTC 4 cut(s) 933, 1026, 1043, 1296
AlwI GGATC 2 cut(s) 709, 722
Aor51HI AGCGCT 1 cut(s) 103
ApeKI GCWGC 5 cut(s) 299, 698, 821, 945, 1205
AspLEI GCGC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 1210
AsuHPI GGTGA 3 cut(s) 509, 517, 1145
AsuII TTCGAA 1 cut(s) 185
BamHI GGATCC 1 cut(s) 714
BanII GRGCYC 1 cut(s) 116
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BbrPI CACGTG 1 cut(s) 1000
BbsI GAAGAC 2 cut(s) 1055, 1235
Bbv12I GWGCWC 2 cut(s) 763, 865
BbvI GCAGC 5 cut(s) 311, 685, 833, 957, 1217
BccI CCATC 4 cut(s) 263, 326, 583, 1164
BciT130I CCWGG 2 cut(s) 25, 963
BclI TGATCA 4 cut(s) 324, 570, 675, 1069
BcnI CCSGG 1 cut(s) 1210
BcoDI GTCTC 4 cut(s) 933, 1026, 1043, 1296
BfaI CTAG 2 cut(s) 363, 669
BfmI CTRYAG 1 cut(s) 1203
BfoI RGCGCY 1 cut(s) 105
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 5 cut(s) 300, 699, 822, 946, 1206
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 5 cut(s) 301, 700, 823, 947, 1207
Bme1390I CCNGG 3 cut(s) 25, 963, 1210
BmiI GGNNCC 2 cut(s) 716, 1009
BmrFI CCNGG 3 cut(s) 25, 963, 1210
BmrI ACTGGG 2 cut(s) 51, 115
BmsI GCATC 1 cut(s) 232
BmuI ACTGGG 2 cut(s) 51, 115
BpiI GAAGAC 2 cut(s) 1055, 1235
Bpu1102I GCTNAGC 1 cut(s) 645
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 1210
BsaAI YACGTR 1 cut(s) 1000
BsaI GGTCTC 1 cut(s) 1026
BsaJI CCNNGG 2 cut(s) 1281, 1295
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 1017, 1208
Bse1I ACTGG 3 cut(s) 46, 121, 822
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 2 cut(s) 25, 963
BseDI CCNNGG 2 cut(s) 1281, 1295
BseGI GGATG 4 cut(s) 247, 327, 598, 1024
BseLI CCNNNNNNNGG 3 cut(s) 16, 1017, 1208
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 6 cut(s) 65, 299, 528, 636, 840, 1043
BseNI ACTGG 3 cut(s) 46, 121, 822
BseXI GCAGC 5 cut(s) 311, 685, 833, 957, 1217
BseYI CCCAGC 1 cut(s) 104
BsgI GTGCAG 2 cut(s) 369, 562
BsiHKAI GWGCWC 2 cut(s) 763, 865
BsiSI CCGG 1 cut(s) 1209
BslI CCNNNNNNNGG 3 cut(s) 16, 1017, 1208
BsmAI GTCTC 4 cut(s) 933, 1026, 1043, 1296
BsmI GAATGC 1 cut(s) 1181
Bso31I GGTCTC 1 cut(s) 1026
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 3 cut(s) 116, 763, 865
Bsp143I GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 2 cut(s) 173, 824
BspCNI CTCAG 6 cut(s) 64, 300, 529, 637, 841, 1042
BspLI GGNNCC 2 cut(s) 716, 1009
BspMAI CTGCAG 1 cut(s) 1207
BspPI GGATC 2 cut(s) 709, 722
BspT104I TTCGAA 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 1026
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 3 cut(s) 46, 121, 822
BssECI CCNNGG 2 cut(s) 1281, 1295
BssMI GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
BssT1I CCWWGG 2 cut(s) 1281, 1295
Bst2UI CCWGG 2 cut(s) 25, 963
Bst4CI ACNGT 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 374
BstBAI YACGTR 1 cut(s) 1000
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 777, 1203
BstDEI CTNAG 6 cut(s) 51, 308, 537, 645, 849, 1029
BstF5I GGATG 4 cut(s) 247, 327, 598, 1024
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 7 cut(s) 327, 573, 678, 717, 1072, 1077, 1108
BstMAI GTCTC 4 cut(s) 933, 1026, 1043, 1296
BstMBI GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
BstMWI GCNNNNNNNGC 6 cut(s) 374, 383, 392, 655, 695, 1254
BstNI CCWGG 2 cut(s) 25, 963
BstNSI RCATGY 3 cut(s) 869, 890, 1094
BstSCI CCNGG 3 cut(s) 23, 961, 1208
BstSFI CTRYAG 1 cut(s) 1203
BstV1I GCAGC 5 cut(s) 311, 685, 833, 957, 1217
BstV2I GAAGAC 2 cut(s) 1055, 1235
BstX2I RGATCY 2 cut(s) 714, 1105
BstXI CCANNNNNNTGG 2 cut(s) 733, 1163
BstYI RGATCY 2 cut(s) 714, 1105
BtsCI GGATG 4 cut(s) 247, 327, 598, 1024
BtsIMutI CAGTG 2 cut(s) 39, 386
Cac8I GCNNGC 2 cut(s) 777, 1203
CfoI GCGC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 484, 1273
CviAII CATG 8 cut(s) 199, 733, 787, 866, 887, 949, 1091, 1118
CviQI GTAC 2 cut(s) 484, 1273
DdeI CTNAG 6 cut(s) 51, 308, 537, 645, 849, 1029
DpnI GATC 7 cut(s) 326, 572, 677, 716, 1071, 1076, 1107
DpnII GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
DraI TTTAAA 1 cut(s) 516
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 1281, 1295
Eco24I GRGCYC 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 1026
Eco47III AGCGCT 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 410
Eco72I CACGTG 1 cut(s) 1000
EcoRII CCWGG 2 cut(s) 23, 961
EcoT14I CCWWGG 2 cut(s) 1281, 1295
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 2 cut(s) 1281, 1295
FaeI CATG 8 cut(s) 202, 736, 790, 869, 890, 952, 1094, 1121
FatI CATG 8 cut(s) 198, 732, 786, 865, 886, 948, 1090, 1117
FbaI TGATCA 4 cut(s) 324, 570, 675, 1069
Fnu4HI GCNGC 5 cut(s) 300, 699, 822, 946, 1206
FokI GGATG 4 cut(s) 254, 334, 605, 1011
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 5 cut(s) 300, 699, 822, 946, 1206
FspBI CTAG 2 cut(s) 363, 669
GlaI GCGC 1 cut(s) 103
GluI GCNGC 5 cut(s) 300, 699, 822, 946, 1206
GsaI CCCAGC 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 105
HapII CCGG 1 cut(s) 1209
HhaI GCGC 1 cut(s) 104
Hin1II CATG 8 cut(s) 202, 736, 790, 869, 890, 952, 1094, 1121
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 2 cut(s) 532, 625
HindII GTYRAC 2 cut(s) 532, 625
HinfI GANTC 1 cut(s) 783
HpaII CCGG 1 cut(s) 1209
HphI GGTGA 3 cut(s) 509, 517, 1145
Hpy166II GTNNAC 3 cut(s) 255, 532, 625
Hpy188I TCNGA 8 cut(s) 54, 70, 146, 318, 570, 917, 1032, 1074
Hpy188III TCNNGA 4 cut(s) 363, 601, 1067, 1078
Hpy8I GTNNAC 3 cut(s) 255, 532, 625
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 3 cut(s) 385, 598, 630
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 2 cut(s) 999, 1275
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 383, 392, 655, 695, 1254
HpyF3I CTNAG 6 cut(s) 51, 308, 537, 645, 849, 1029
HpySE526I ACGT 2 cut(s) 999, 1275
Hsp92II CATG 8 cut(s) 202, 736, 790, 869, 890, 952, 1094, 1121
HspAI GCGC 1 cut(s) 102
Ksp22I TGATCA 4 cut(s) 324, 570, 675, 1069
Kzo9I GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
LmnI GCTCC 4 cut(s) 641, 860, 895, 1007
Lsp1109I GCAGC 5 cut(s) 311, 685, 833, 957, 1217
LweI GCATC 1 cut(s) 232
MaeI CTAG 2 cut(s) 363, 669
MaeII ACGT 2 cut(s) 999, 1275
MaeIII GTNAC 1 cut(s) 37
MalI GATC 7 cut(s) 326, 572, 677, 716, 1071, 1076, 1107
MboI GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
MboII GAAGA 8 cut(s) 372, 920, 940, 1025, 1045, 1055, 1142, 1240
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 2 cut(s) 714, 1105
MhlI GDGCHC 3 cut(s) 116, 763, 865
MluCI AATT 4 cut(s) 830, 956, 1132, 1176
MmeI TCCRAC 4 cut(s) 742, 750, 964, 1284
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 1 cut(s) 1161
MspA1I CMGCKG 1 cut(s) 824
MspI CCGG 1 cut(s) 1209
MspR9I CCNGG 3 cut(s) 25, 963, 1210
MunI CAATTG 1 cut(s) 1176
Mva1269I GAATGC 1 cut(s) 1181
MvaI CCWGG 2 cut(s) 25, 963
MwoI GCNNNNNNNGC 6 cut(s) 374, 383, 392, 655, 695, 1254
NciI CCSGG 1 cut(s) 1210
NdeII GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
NlaIII CATG 8 cut(s) 202, 736, 790, 869, 890, 952, 1094, 1121
NlaIV GGNNCC 2 cut(s) 716, 1009
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 3 cut(s) 869, 890, 1094
NspV TTCGAA 1 cut(s) 185
PciI ACATGT 2 cut(s) 865, 1090
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 1 cut(s) 1181
PfeI GAWTC 1 cut(s) 783
PfoI TCCNGGA 1 cut(s) 23
PkrI GCNGC 5 cut(s) 301, 700, 823, 947, 1207
PmaCI CACGTG 1 cut(s) 1000
PmlI CACGTG 1 cut(s) 1000
Ppu21I YACGTR 1 cut(s) 1000
PscI ACATGT 2 cut(s) 865, 1090
Psp6I CCWGG 2 cut(s) 23, 961
PspCI CACGTG 1 cut(s) 1000
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 2 cut(s) 23, 961
PspN4I GGNNCC 2 cut(s) 716, 1009
PstI CTGCAG 1 cut(s) 1207
PsuI RGATCY 2 cut(s) 714, 1105
RsaI GTAC 2 cut(s) 485, 1274
RsaNI GTAC 2 cut(s) 484, 1273
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 5 cut(s) 300, 699, 822, 946, 1206
Sau3AI GATC 7 cut(s) 324, 570, 675, 714, 1069, 1074, 1105
ScrFI CCNGG 3 cut(s) 25, 963, 1210
SduI GDGCHC 3 cut(s) 116, 763, 865
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 1203
SfuI TTCGAA 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 4 cut(s) 830, 956, 1132, 1176
SsiI CCGC 2 cut(s) 173, 824
SspI AATATT 1 cut(s) 550
SspMI CTAG 2 cut(s) 363, 669
StyD4I CCNGG 3 cut(s) 23, 961, 1208
StyI CCWWGG 2 cut(s) 1281, 1295
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 2 cut(s) 1002, 1278
TaqI TCGA 6 cut(s) 185, 222, 260, 422, 488, 600
TasI AATT 4 cut(s) 830, 956, 1132, 1176
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 1 cut(s) 783
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TscAI CASTG 2 cut(s) 46, 393
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 5 cut(s) 299, 698, 821, 945, 1205
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 6 cut(s) 187, 407, 609, 721, 775, 965
TspRI CASTG 2 cut(s) 46, 393
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 3 cut(s) 869, 890, 1094
XspI CTAG 2 cut(s) 363, 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.