Rmu_sc0016164.1_g000017

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016164.1
OG0001542
Physical Location & Seq
Forward (+)
55157 .. 57241
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016164.1_g000017.1.cds

Sequence Viewer

Length: 2085 bp
atgagttttcaacccttgcgcctttttggcggccaaattctgaaagatgagtccaccagcttggcgcaagtcatccaaacttacgcaaaaaccaagcaccttaacagaggcaaagagctccacgctcacctcctacgcactcattacccactatgcattttcctcaccaaccacctcctcaacatgtactccaaatgtgggcgcgtcgattacgccctcaaggttttcgaccaaatgtctcaccgaaacttggtttcctggactgcaatggtaactgggttttctcagaacttgaggttctccgagagtttcaaggccttttcccagatgagaattgctggggagagcccgacccagtttgcatttgcaagtgtcatcagagcttgtgtggttcttgggtcggttgagattgggaggcagctgcattctcttgctttgaaattgggcttggcttgtgagctgtttgtgggtagtaacttggcggatatgtattcgaaatgtgggttcatggttgaggcttgtaaggttttcgaggaaatgccgagtaaggatgctgtatcgtggacttcgatgattgatgggtatgcaaagagtggggattttgaggcagctttactgacttataagaggatggttagtgatgggattggtatagataaacatgttgtttctagtgcattgagtgcttgttctgcactgaaggcttgtcagtttggaaagtgtgtccattcgacaggtgtgaagttgggattggaagtagaggttgctgtgggaaatgctctggttgatatgtactcgaaatcaggtgatatggagagtgctttaaatgtgtttcgggttgaccctgagtgcagaaatattgtgtcgtattcctctctgatcaatggttatgttgagatggatgaaatcgagaaggcttttagtgtgtttgttgacttacagagaaggggagctgagcctaatcagttcactttctctagcttgatcaaggcttgtgccaaccaagctgcacttgaacaagggatccaacttcatgctcaggtggtcaagtttaattttgatagagatgattttgtgtcttctgttcttgttgatatgtatgggaaatgtgggttacttgatcattcaatgcaagtttttgacgaggtacccaccccaactgaagttgcatggaattcattgctaagtgtatttgcagttcacggcttaggaaatgacgccttgaaaacttttagtagaatggtccaggcaggagtgaaaccaaatgcaataacatttatcagtcttttaacaggctgtagccattctggattggtagaggaaggtttaaagtacttttactctctggagaaaacatatggcatagtacctagagctggacattactcttgcgttattgatttacttggcagggctggaaggcttgaagaggccgaagaattcattaacagcatgccaatgcagccaaatgcttttggatggtgctcttttcttggtgcttgcacaattcatggtgataaagagaggggcaaactggcagcggagaaattgatacagcttgaacctgagaatagtggagcacatgtgttgctttcaactatatatgcaaaggagcagaaatgggaagatttcaggggtgtgaggaggataatgagggacagcagcatgaagaaattgcccggttatagttggattgacattggaaacaaaacacacacatttggagccgaagattggtctcatcctcagaagaaagaaatatatgagaagcttgatagtcttcttgatcagataaagaaagctggatatgttccaaatacagatcttgttcttcatgatatggacgacaattcaaaggtagagcttcttcaccatcatagtgagaggatagcgattgcatttgcactgataagtatgcctgctgggaagccaatcattgtgaagaaaaatataagggtgtgcttggattgccattctgcaatcaagtacatctccttggttgttggaagaaagattattgttagggataatactcggtttcaccatttcgcagatgggttgtgttcatgtggagattactggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

694

Amino Acids

77.26

Weight (kDa)

6.93

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
Acc65I GGTACC 1 cut(s) 1147
AccB1I GGYRCC 1 cut(s) 1147
AccII CGCG 1 cut(s) 204
AciI CCGC 3 cut(s) 30, 482, 1541
AclWI GGATC 2 cut(s) 1018, 1031
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 3 cut(s) 36, 1174, 1439
AcuI CTGAAG 2 cut(s) 719, 1182
AcyI GRCGYC 1 cut(s) 1218
AfaI GTAC 6 cut(s) 188, 794, 1149, 1334, 1368, 1988
AfiI CCNNNNNNNGG 4 cut(s) 198, 250, 1376, 1734
AflIII ACRYGT 3 cut(s) 183, 661, 1582
AhdI GACNNNNNGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 257, 1245
AjuI GAANNNNNNNTTGG 4 cut(s) 431, 463, 734, 766
AloI GAACNNNNNNTCC 2 cut(s) 1008, 1040
Alw21I GWGCWC 3 cut(s) 120, 1487, 1582
Alw26I GTCTC 2 cut(s) 243, 1743
AlwI GGATC 2 cut(s) 1018, 1031
AoxI GGCC 3 cut(s) 31, 315, 1431
ApeKI GCWGC 7 cut(s) 418, 421, 608, 1007, 1462, 1538, 1662
ApoI RAATTY 3 cut(s) 36, 1174, 1439
Asp718I GGTACC 1 cut(s) 1147
AspLEI GCGC 3 cut(s) 21, 67, 204
AspS9I GGNCC 1 cut(s) 1243
AsuC2I CCSGG 1 cut(s) 1680
AsuHPI GGTGA 7 cut(s) 119, 157, 233, 818, 1526, 1862, 2033
AsuII TTCGAA 1 cut(s) 494
AvaII GGWCC 1 cut(s) 1243
BamHI GGATCC 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 1147
BanII GRGCYC 2 cut(s) 120, 350
BarI GAAGNNNNNNTAC 2 cut(s) 1851, 1883
BbsI GAAGAC 2 cut(s) 1071, 1772
Bbv12I GWGCWC 3 cut(s) 120, 1487, 1582
BbvI GCAGC 7 cut(s) 408, 430, 620, 994, 1474, 1550, 1674
BccI CCATC 7 cut(s) 572, 625, 635, 892, 1473, 1881, 2048
BceAI ACGGC 1 cut(s) 1219
BciT130I CCWGG 2 cut(s) 259, 1247
BclI TGATCA 4 cut(s) 879, 984, 1120, 1786
BcnI CCSGG 1 cut(s) 1680
BcoDI GTCTC 2 cut(s) 243, 1743
BfaI CTAG 3 cut(s) 672, 978, 1371
BfmI CTRYAG 1 cut(s) 1297
BglI GCCNNNNNGGC 1 cut(s) 27
BglII AGATCT 1 cut(s) 1822
BisI GCNGC 8 cut(s) 31, 419, 422, 609, 1008, 1463, 1539, 1663
BlpI GCTNAGC 1 cut(s) 954
BlsI GCNGC 8 cut(s) 32, 420, 423, 610, 1009, 1464, 1540, 1664
BmcAI AGTACT 1 cut(s) 1334
Bme1390I CCNGG 3 cut(s) 259, 1247, 1680
Bme18I GGWCC 1 cut(s) 1243
BmeRI GACNNNNNGTC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 1243
BmiI GGNNCC 3 cut(s) 1025, 1149, 1726
BmrFI CCNGG 3 cut(s) 259, 1247, 1680
BmrI ACTGGG 2 cut(s) 285, 349
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 2 cut(s) 285, 349
BpiI GAAGAC 2 cut(s) 1071, 1772
BpmI CTGGAG 1 cut(s) 1367
Bpu10I CCTNAGC 2 cut(s) 1038, 1207
Bpu1102I GCTNAGC 1 cut(s) 954
Bpu14I TTCGAA 1 cut(s) 494
BpuEI CTTGAG 2 cut(s) 203, 313
BpuMI CCSGG 1 cut(s) 1680
BsaHI GRCGYC 1 cut(s) 1218
BsaI GGTCTC 1 cut(s) 1743
BsaJI CCNNGG 1 cut(s) 1995
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 1569, 1599
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 250, 1376, 1734
Bse1I ACTGG 4 cut(s) 280, 355, 1539, 2084
Bse3DI GCAATG 2 cut(s) 273, 1178
BseBI CCWGG 2 cut(s) 259, 1247
BseDI CCNNGG 1 cut(s) 1995
BseGI GGATG 6 cut(s) 72, 556, 636, 907, 1484, 1741
BseLI CCNNNNNNNGG 4 cut(s) 198, 250, 1376, 1734
BseMI GCAATG 2 cut(s) 273, 1178
BseMII CTCAG 6 cut(s) 299, 837, 945, 1052, 1557, 1760
BseNI ACTGG 4 cut(s) 280, 355, 1539, 2084
BseRI GAGGAG 2 cut(s) 167, 1657
BseXI GCAGC 7 cut(s) 408, 430, 620, 994, 1474, 1550, 1674
BseYI CCCAGC 2 cut(s) 338, 1922
BsgI GTGCAG 3 cut(s) 678, 871, 993
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 3 cut(s) 33, 317, 1433
BshNI GGYRCC 1 cut(s) 1147
BsiHKAI GWGCWC 3 cut(s) 120, 1487, 1582
BsiSI CCGG 1 cut(s) 1680
BslFI GGGAC 1 cut(s) 1670
BslI CCNNNNNNNGG 4 cut(s) 198, 250, 1376, 1734
BsmAI GTCTC 2 cut(s) 243, 1743
BsmFI GGGAC 1 cut(s) 1670
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 3 cut(s) 33, 317, 1433
Bso31I GGTCTC 1 cut(s) 1743
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 4 cut(s) 120, 350, 1487, 1582
Bsp143I GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
Bsp1720I GCTNAGC 1 cut(s) 954
BspACI CCGC 3 cut(s) 30, 482, 1541
BspANI GGCC 3 cut(s) 33, 317, 1433
BspCNI CTCAG 6 cut(s) 298, 838, 946, 1051, 1558, 1759
BspFNI CGCG 1 cut(s) 204
BspHI TCATGA 1 cut(s) 1834
BspLI GGNNCC 3 cut(s) 1025, 1149, 1726
BspPI GGATC 2 cut(s) 1018, 1031
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 1147
BspTNI GGTCTC 1 cut(s) 1743
BsrDI GCAATG 2 cut(s) 273, 1178
BsrI ACTGG 4 cut(s) 280, 355, 1539, 2084
BssECI CCNNGG 1 cut(s) 1995
BssMI GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
BssNI GRCGYC 1 cut(s) 1218
BssT1I CCWWGG 1 cut(s) 1995
Bst2UI CCWGG 2 cut(s) 259, 1247
Bst6I CTCTTC 1 cut(s) 1422
BstACI GRCGYC 1 cut(s) 1218
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 3 cut(s) 1454, 1501, 1920
BstDEI CTNAG 8 cut(s) 285, 846, 954, 1038, 1184, 1207, 1566, 1746
BstF5I GGATG 6 cut(s) 72, 556, 636, 907, 1484, 1741
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 3 cut(s) 21, 67, 204
BstKTI GATC 6 cut(s) 882, 987, 1026, 1123, 1789, 1825
BstMAI GTCTC 2 cut(s) 243, 1743
BstMBI GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
BstMWI GCNNNNNNNGC 7 cut(s) 27, 683, 692, 701, 1004, 1462, 1968
BstNI CCWGG 2 cut(s) 259, 1247
BstNSI RCATGY 4 cut(s) 187, 665, 1456, 1586
BstSCI CCNGG 3 cut(s) 257, 1245, 1678
BstSFI CTRYAG 1 cut(s) 1297
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 7 cut(s) 408, 430, 620, 994, 1474, 1550, 1674
BstV2I GAAGAC 2 cut(s) 1071, 1772
BstX2I RGATCY 2 cut(s) 1023, 1822
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 2 cut(s) 1023, 1822
BsuRI GGCC 3 cut(s) 33, 317, 1433
BtsCI GGATG 6 cut(s) 72, 556, 636, 907, 1484, 1741
BtsIMutI CAGTG 2 cut(s) 695, 1904
Cac8I GCNNGC 3 cut(s) 1454, 1501, 1920
CciI TCATGA 1 cut(s) 1834
CfoI GCGC 3 cut(s) 21, 67, 204
Cfr13I GGNCC 1 cut(s) 1243
CseI GACGC 2 cut(s) 193, 1226
Csp6I GTAC 6 cut(s) 187, 793, 1148, 1333, 1367, 1987
CviQI GTAC 6 cut(s) 187, 793, 1148, 1333, 1367, 1987
DdeI CTNAG 8 cut(s) 285, 846, 954, 1038, 1184, 1207, 1566, 1746
DpnI GATC 6 cut(s) 881, 986, 1025, 1122, 1788, 1824
DpnII GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
DraI TTTAAA 2 cut(s) 825, 1329
DriI GACNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 1422
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 1422
EciI GGCGGA 1 cut(s) 497
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 1995
Eco147I AGGCCT 1 cut(s) 317
Eco24I GRGCYC 2 cut(s) 120, 350
Eco31I GGTCTC 1 cut(s) 1743
Eco47I GGWCC 1 cut(s) 1243
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 719, 1182
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 2 cut(s) 1174, 1439
EcoRII CCWGG 2 cut(s) 257, 1245
EcoT14I CCWWGG 1 cut(s) 1995
EcoT22I ATGCAT 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 120, 350
ErhI CCWWGG 1 cut(s) 1995
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 1670
FauNDI CATATG 1 cut(s) 1357
FbaI TGATCA 4 cut(s) 879, 984, 1120, 1786
Fnu4HI GCNGC 8 cut(s) 31, 419, 422, 609, 1008, 1463, 1539, 1663
FokI GGATG 6 cut(s) 59, 563, 643, 914, 1491, 1728
FriOI GRGCYC 2 cut(s) 120, 350
Fsp4HI GCNGC 8 cut(s) 31, 419, 422, 609, 1008, 1463, 1539, 1663
FspBI CTAG 3 cut(s) 672, 978, 1371
GlaI GCGC 3 cut(s) 20, 66, 203
GluI GCNGC 8 cut(s) 31, 419, 422, 609, 1008, 1463, 1539, 1663
GsaI CCCAGC 2 cut(s) 342, 1926
GsuI CTGGAG 1 cut(s) 1367
HaeIII GGCC 3 cut(s) 33, 317, 1433
HapII CCGG 1 cut(s) 1680
HgaI GACGC 2 cut(s) 193, 1226
HhaI GCGC 3 cut(s) 21, 67, 204
Hin1I GRCGYC 1 cut(s) 1218
Hin6I GCGC 3 cut(s) 19, 65, 202
HinP1I GCGC 3 cut(s) 19, 65, 202
HincII GTYRAC 2 cut(s) 841, 934
HindII GTYRAC 2 cut(s) 841, 934
HindIII AAGCTT 1 cut(s) 1769
HinfI GANTC 1 cut(s) 50
HpaII CCGG 1 cut(s) 1680
HphI GGTGA 7 cut(s) 119, 157, 233, 818, 1526, 1862, 2033
Hpy166II GTNNAC 6 cut(s) 54, 564, 841, 934, 969, 1201
Hpy188I TCNGA 7 cut(s) 42, 288, 304, 380, 879, 1749, 1791
Hpy188III TCNNGA 5 cut(s) 910, 1308, 1346, 1784, 1835
Hpy8I GTNNAC 6 cut(s) 54, 564, 841, 934, 969, 1201
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 5 cut(s) 694, 907, 939, 1316, 1413
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 683, 692, 701, 1004, 1462, 1968
HpyF3I CTNAG 8 cut(s) 285, 846, 954, 1038, 1184, 1207, 1566, 1746
Hsp92I GRCGYC 1 cut(s) 1218
HspAI GCGC 3 cut(s) 19, 65, 202
KpnI GGTACC 1 cut(s) 1151
Ksp22I TGATCA 4 cut(s) 879, 984, 1120, 1786
Kzo9I GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
LmnI GCTCC 5 cut(s) 123, 950, 1577, 1612, 1724
Lsp1109I GCAGC 7 cut(s) 408, 430, 620, 994, 1474, 1550, 1674
LweI GCATC 1 cut(s) 541
MaeI CTAG 3 cut(s) 672, 978, 1371
MaeIII GTNAC 3 cut(s) 271, 473, 1113
MalI GATC 6 cut(s) 881, 986, 1025, 1122, 1788, 1824
MboI GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
MflI RGATCY 2 cut(s) 1023, 1822
MhlI GDGCHC 4 cut(s) 120, 350, 1487, 1582
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 3 cut(s) 1051, 1670, 1984
Mph1103I ATGCAT 1 cut(s) 158
MseI TTAA 6 cut(s) 102, 824, 1053, 1289, 1328, 1446
MslI CAYNNNNRTG 2 cut(s) 1457, 1878
MspA1I CMGCKG 2 cut(s) 421, 1541
MspI CCGG 1 cut(s) 1680
MspR9I CCNGG 3 cut(s) 259, 1247, 1680
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 2 cut(s) 259, 1247
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 7 cut(s) 27, 683, 692, 701, 1004, 1462, 1968
NciI CCSGG 1 cut(s) 1680
NdeI CATATG 1 cut(s) 1357
NdeII GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
NlaIV GGNNCC 3 cut(s) 1025, 1149, 1726
NmeAIII GCCGAG 1 cut(s) 567
NsiI ATGCAT 1 cut(s) 158
NspI RCATGY 4 cut(s) 187, 665, 1456, 1586
NspV TTCGAA 1 cut(s) 494
PaeI GCATGC 1 cut(s) 1456
PagI TCATGA 1 cut(s) 1834
PceI AGGCCT 1 cut(s) 317
PciI ACATGT 3 cut(s) 183, 661, 1582
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PctI GAATGC 1 cut(s) 424
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 8 cut(s) 32, 420, 423, 610, 1009, 1464, 1540, 1664
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PscI ACATGT 3 cut(s) 183, 661, 1582
PsiI TTATAA 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 257, 1245
PspFI CCCAGC 2 cut(s) 338, 1922
PspGI CCWGG 2 cut(s) 257, 1245
PspN4I GGNNCC 3 cut(s) 1025, 1149, 1726
PspPI GGNCC 1 cut(s) 1243
PsrI GAACNNNNNNTAC 2 cut(s) 1182, 1214
PsuI RGATCY 2 cut(s) 1023, 1822
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 6 cut(s) 188, 794, 1149, 1334, 1368, 1988
RsaNI GTAC 6 cut(s) 187, 793, 1148, 1333, 1367, 1987
RseI CAYNNNNRTG 2 cut(s) 1457, 1878
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 6 cut(s) 102, 824, 1053, 1289, 1328, 1446
SatI GCNGC 8 cut(s) 31, 419, 422, 609, 1008, 1463, 1539, 1663
Sau3AI GATC 6 cut(s) 879, 984, 1023, 1120, 1786, 1822
Sau96I GGNCC 1 cut(s) 1243
ScaI AGTACT 1 cut(s) 1334
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 259, 1247, 1680
SduI GDGCHC 4 cut(s) 120, 350, 1487, 1582
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 1297
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 1 cut(s) 1243
SmiMI CAYNNNNRTG 2 cut(s) 1457, 1878
SmlI CTYRAG 2 cut(s) 218, 292
SmoI CTYRAG 2 cut(s) 218, 292
SphI GCATGC 1 cut(s) 1456
SseBI AGGCCT 1 cut(s) 317
SsiI CCGC 3 cut(s) 30, 482, 1541
SspI AATATT 1 cut(s) 859
SspMI CTAG 3 cut(s) 672, 978, 1371
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 317
StyD4I CCNGG 3 cut(s) 257, 1245, 1678
StyI CCWWGG 1 cut(s) 1995
TaqI TCGA 8 cut(s) 207, 228, 494, 531, 569, 731, 797, 909
TatI WGTACW 4 cut(s) 186, 792, 1332, 1986
TauI GCSGC 1 cut(s) 33
Tru1I TTAA 6 cut(s) 102, 824, 1053, 1289, 1328, 1446
Tru9I TTAA 6 cut(s) 102, 824, 1053, 1289, 1328, 1446
TscAI CASTG 2 cut(s) 702, 1911
TseI GCWGC 7 cut(s) 418, 421, 608, 1007, 1462, 1538, 1662
TspDTI ATGAA 9 cut(s) 496, 918, 1022, 1167, 1432, 1499, 1682, 1823, 2055
TspRI CASTG 2 cut(s) 702, 1911
VpaK11BI GGWCC 1 cut(s) 1243
XapI RAATTY 3 cut(s) 36, 1174, 1439
XceI RCATGY 4 cut(s) 187, 665, 1456, 1586
XcmI CCANNNNNNNNNTGG 1 cut(s) 2051
XspI CTAG 3 cut(s) 672, 978, 1371
ZrmI AGTACT 1 cut(s) 1334
Zsp2I ATGCAT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.