MD12G1227600.v1.1

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
30308630 .. 30314219
5590 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1227600.v1.1.491

Sequence Viewer

Length: 1746 bp
ATGGGAGGACGCCTTTCGGACTTGAGGGGAGGCAAGGCCGCCGTGGGCACCGAACCGGACGCCGAGAACGACGGCGCTCACAACGATGCCATTGAATTCTTTTACAGGGCGCATGGTGCTTACCCGCATTTTACGCAACTCGAGTTATCCTTTTCAGCATCCAAGCTGCTTGATCGTGATCTCTTTTCCAAGAGTGATCCTATGGTGGTTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTCGGGAGAACTGAAGTCATACTGAACAATTTAAATCCGGAATGGATACAGAACGTTTCAGTAGCTTATCAGTTCGAGATGGTGCAGAATTTGATATTCTGTGTCTACGATGTTGATACACAATTCCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCAAGAGTTTCTTGGAGAAGGCAGTTGTGACCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTCAACAATGACTCTGAAAGGCAAAAAGGGGCATGGAGGTTTGAGAAACTTTGGAACACTCACCGTGCATGCCGAGGAAACTATTGCTTCTAGGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTCTTTTCTAAAAGCGATCCTTTCTTAATAATATCAAGAACTGTTGAGACTGGGGATTCTATTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAACCCGACCTGGAAACCAGTATGCCTGGGTATGCAACAGTTCAGAAGCAAGGACGCCCCGCTGGTTATTGAGTGTTTTGATTTCAACAGCAATGGCAAACACATTCTTATTGGGAGACTCCGAAAATCAGTAGCAGACCTTGAAAAACTACTCAGAGAAGAACGTGGGGCGAACATGTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGAGTCAGCTATTTGTGGATCAGTTTTGTGAGAAAGAACAATTCACCTTTGTTGACTATATATCTAGTGGGTTTGAGCTTAACTGCATGGTTGCAGTTGATTTTACGGCTTCAAATGGAGATCCTAGAAATCCCGAGTCTTTGCACTACATTGATCCTAATGGCGGGCTGAATTCTTACCAGCAGGCTATAACAGAAGTAGGACCAGTCATTCAGTTCTACGATGCTGATAAGAGGTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCATACTGCTTCAACTTGAATGGAGATTCAAGTGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCAACGCGATATTGCTCTGTCAGAACCGACGTTGTTTGGCCCAGTGGTTAACAGGGCTGCTAAAATTGCCACCAGGTCCCTCTCGTCCAACAAGTACTACGTCTTGGTCATTATAACGGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAATGCGGATTTCAAACAAATGAAGATCCTTGACGCTGACAACGGGCAACGATTAAAAAGTTCTACAGGTCACATAGCTACGCGTGACATTGTCCAGTTCATTCCAATGAGAGATGTGCAGAGTGGGCAGATTTCTGTTGTTCAAGGTCTTTTGGAAGAGCTGCCCGGACAGTTTTTGAGTTACATGCGTTCCATGGACATCAAGCCGCTCACCTTCAATGCCGCTGCCCAAGCATCACCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

582

Amino Acids

64.23

Weight (kDa)

5.45

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 52 - 158 8.2e-13 C2 domain
C2 PF00168 196 - 286 1.2e-11 C2 domain
Copine PF07002 356 - 569 7.8e-76 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1406
AccB1I GGYRCC 1 cut(s) 47
AccBSI CCGCTC 1 cut(s) 1708
AccI GTMKAC 2 cut(s) 213, 351
AccII CGCG 2 cut(s) 1299, 1582
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 9 cut(s) 39, 125, 586, 770, 908, 1092, 1505, 1706, 1722
AclI AACGTT 1 cut(s) 300
AclWI GGATC 7 cut(s) 191, 414, 623, 954, 1043, 1076, 1519
AcsI RAATTY 5 cut(s) 95, 334, 397, 581, 1099
AcuI CTGAAG 2 cut(s) 279, 711
AcyI GRCGYC 3 cut(s) 10, 60, 765
AfaI GTAC 2 cut(s) 381, 1388
AfiI CCNNNNNNNGG 2 cut(s) 1091, 1175
AflIII ACRYGT 2 cut(s) 885, 1580
AjnI CCWGG 3 cut(s) 719, 735, 1364
AluBI AGCT 7 cut(s) 166, 238, 311, 937, 1006, 1577, 1660
AluI AGCT 7 cut(s) 166, 238, 311, 937, 1006, 1577, 1660
Alw26I GTCTC 2 cut(s) 653, 820
AlwI GGATC 7 cut(s) 191, 414, 623, 954, 1043, 1076, 1519
Ama87I CYCGRG 3 cut(s) 140, 248, 1061
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 2 cut(s) 36, 1330
ApeKI GCWGC 5 cut(s) 166, 1277, 1349, 1660, 1724
ApoI RAATTY 5 cut(s) 95, 334, 397, 581, 1099
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
AspLEI GCGC 2 cut(s) 77, 112
AspS9I GGNCC 3 cut(s) 1130, 1331, 1368
AsuC2I CCSGG 1 cut(s) 1665
AsuHPI GGTGA 5 cut(s) 523, 964, 1465, 1702, 1728
AvaI CYCGRG 3 cut(s) 140, 248, 1061
AvaII GGWCC 2 cut(s) 1130, 1368
BaeGI GKGCMC 2 cut(s) 50, 595
BanI GGYRCC 1 cut(s) 47
BauI CACGAG 1 cut(s) 903
BbvI GCAGC 5 cut(s) 153, 1264, 1336, 1647, 1711
BccI CCATC 4 cut(s) 224, 319, 904, 1406
BceAI ACGGC 4 cut(s) 26, 88, 1050, 1209
BciT130I CCWGG 3 cut(s) 721, 737, 1366
BciVI GTATCC 1 cut(s) 285
BcnI CCSGG 1 cut(s) 1665
BcoDI GTCTC 2 cut(s) 653, 820
BfaI CTAG 4 cut(s) 239, 561, 993, 1053
BfmI CTRYAG 2 cut(s) 570, 1563
BfoI RGCGCY 1 cut(s) 78
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 610
BisI GCNGC 8 cut(s) 39, 167, 1278, 1350, 1661, 1706, 1722, 1725
BlsI GCNGC 8 cut(s) 40, 168, 1279, 1351, 1662, 1707, 1723, 1726
BmcAI AGTACT 1 cut(s) 1388
Bme1390I CCNGG 4 cut(s) 721, 737, 1366, 1665
Bme18I GGWCC 2 cut(s) 1130, 1368
BmeT110I CYCGRG 3 cut(s) 140, 248, 1061
BmgT120I GGNCC 3 cut(s) 1130, 1331, 1368
BmiI GGNNCC 2 cut(s) 49, 1370
BmrFI CCNGG 4 cut(s) 721, 737, 1366, 1665
BmrI ACTGGG 2 cut(s) 672, 1328
BmsI GCATC 6 cut(s) 76, 167, 1141, 1435, 1469, 1742
BmuI ACTGGG 2 cut(s) 672, 1328
BpuEI CTTGAG 2 cut(s) 43, 203
BpuMI CCSGG 1 cut(s) 1665
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 3 cut(s) 10, 60, 765
BsaJI CCNNGG 4 cut(s) 42, 543, 736, 1692
BsaWI WCCGGW 2 cut(s) 55, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 1091, 1175
Bse1I ACTGG 5 cut(s) 667, 728, 1133, 1334, 1594
Bse3DI GCAATG 1 cut(s) 808
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 3 cut(s) 721, 737, 1366
BseDI CCNNGG 4 cut(s) 42, 543, 736, 1692
BseGI GGATG 2 cut(s) 158, 1417
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 1091, 1175
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 2 cut(s) 877, 1239
BseNI ACTGG 5 cut(s) 667, 728, 1133, 1334, 1594
BseSI GKGCMC 2 cut(s) 50, 595
BseXI GCAGC 5 cut(s) 153, 1264, 1336, 1647, 1711
BsgI GTGCAG 2 cut(s) 350, 1637
Bsh1236I CGCG 2 cut(s) 1299, 1582
BshFI GGCC 2 cut(s) 38, 1332
BshNI GGYRCC 1 cut(s) 47
BsiHKCI CYCGRG 3 cut(s) 140, 248, 1061
BsiSI CCGG 3 cut(s) 56, 284, 1665
BslFI GGGAC 1 cut(s) 1354
BslI CCNNNNNNNGG 2 cut(s) 1091, 1175
BsmAI GTCTC 2 cut(s) 653, 820
BsmFI GGGAC 1 cut(s) 1354
BsnI GGCC 2 cut(s) 38, 1332
BsoBI CYCGRG 3 cut(s) 140, 248, 1061
Bsp1286I GDGCHC 2 cut(s) 50, 595
Bsp13I TCCGGA 1 cut(s) 283
Bsp19I CCATGG 1 cut(s) 1692
BspACI CCGC 9 cut(s) 39, 125, 586, 770, 908, 1092, 1505, 1706, 1722
BspANI GGCC 2 cut(s) 38, 1332
BspCNI CTCAG 2 cut(s) 876, 1240
BspEI TCCGGA 1 cut(s) 283
BspFNI CGCG 2 cut(s) 1299, 1582
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 2 cut(s) 49, 1370
BspPI GGATC 7 cut(s) 191, 414, 623, 954, 1043, 1076, 1519
BspQI GCTCTTC 1 cut(s) 1650
BspT107I GGYRCC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 1708
BsrDI GCAATG 1 cut(s) 808
BsrI ACTGG 5 cut(s) 667, 728, 1133, 1334, 1594
BssECI CCNNGG 4 cut(s) 42, 543, 736, 1692
BssNAI GTATAC 1 cut(s) 214
BssNI GRCGYC 3 cut(s) 10, 60, 765
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 1692
Bst1107I GTATAC 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 3 cut(s) 721, 737, 1366
Bst4CI ACNGT 5 cut(s) 535, 655, 750, 1207, 1671
Bst6I CTCTTC 2 cut(s) 923, 1650
BstACI GRCGYC 3 cut(s) 10, 60, 765
BstAPI GCANNNNNTGC 1 cut(s) 1739
BstC8I GCNNGC 3 cut(s) 540, 1094, 1113
BstDEI CTNAG 2 cut(s) 863, 1248
BstDSI CCRYGG 2 cut(s) 42, 1692
BstF5I GGATG 2 cut(s) 158, 1417
BstFNI CGCG 2 cut(s) 1299, 1582
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 112
BstMAI GTCTC 2 cut(s) 653, 820
BstMWI GCNNNNNNNGC 7 cut(s) 116, 133, 1283, 1358, 1624, 1730, 1739
BstNI CCWGG 3 cut(s) 721, 737, 1366
BstNSI RCATGY 3 cut(s) 542, 889, 1687
BstSCI CCNGG 4 cut(s) 719, 735, 1364, 1663
BstSFI CTRYAG 2 cut(s) 570, 1563
BstSLI GKGCMC 2 cut(s) 50, 595
BstUI CGCG 2 cut(s) 1299, 1582
BstV1I GCAGC 5 cut(s) 153, 1264, 1336, 1647, 1711
BstX2I RGATCY 3 cut(s) 610, 1048, 1524
BstYI RGATCY 3 cut(s) 610, 1048, 1524
BstZ17I GTATAC 1 cut(s) 214
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 38, 1332
BtgI CCRYGG 2 cut(s) 42, 1692
BtsCI GGATG 2 cut(s) 158, 1417
BtsIMutI CAGTG 2 cut(s) 1341, 1422
Cac8I GCNNGC 3 cut(s) 540, 1094, 1113
CciI TCATGA 1 cut(s) 913
CfoI GCGC 2 cut(s) 77, 112
Cfr13I GGNCC 3 cut(s) 1130, 1331, 1368
CseI GACGC 4 cut(s) 18, 68, 773, 1541
CsiI ACCWGGT 1 cut(s) 1364
Csp6I GTAC 2 cut(s) 380, 1387
CviAII CATG 8 cut(s) 113, 503, 539, 886, 914, 1015, 1684, 1693
CviQI GTAC 2 cut(s) 380, 1387
DdeI CTNAG 2 cut(s) 863, 1248
DraI TTTAAA 2 cut(s) 279, 711
Eam1104I CTCTTC 2 cut(s) 923, 1650
EarI CTCTTC 2 cut(s) 923, 1650
Eco130I CCWWGG 1 cut(s) 1692
Eco47I GGWCC 2 cut(s) 1130, 1368
Eco57I CTGAAG 2 cut(s) 279, 711
Eco88I CYCGRG 3 cut(s) 140, 248, 1061
EcoO109I RGGNCCY 1 cut(s) 1368
EcoRI GAATTC 2 cut(s) 95, 1099
EcoRII CCWGG 3 cut(s) 719, 735, 1364
EcoT14I CCWWGG 1 cut(s) 1692
ErhI CCWWGG 1 cut(s) 1692
FaeI CATG 8 cut(s) 116, 506, 542, 889, 917, 1018, 1687, 1696
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 616, 648
FaqI GGGAC 1 cut(s) 1354
FatI CATG 8 cut(s) 112, 502, 538, 885, 913, 1014, 1683, 1692
FauI CCCGC 4 cut(s) 132, 777, 901, 1085
FblI GTMKAC 2 cut(s) 213, 351
Fnu4HI GCNGC 8 cut(s) 39, 167, 1278, 1350, 1661, 1706, 1722, 1725
FokI GGATG 2 cut(s) 145, 1424
Fsp4HI GCNGC 8 cut(s) 39, 167, 1278, 1350, 1661, 1706, 1722, 1725
FspBI CTAG 4 cut(s) 239, 561, 993, 1053
GlaI GCGC 2 cut(s) 76, 111
GluI GCNGC 8 cut(s) 39, 167, 1278, 1350, 1661, 1706, 1722, 1725
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 38, 1332
HapII CCGG 3 cut(s) 56, 284, 1665
HgaI GACGC 4 cut(s) 18, 68, 773, 1541
HhaI GCGC 2 cut(s) 77, 112
Hin1I GRCGYC 3 cut(s) 10, 60, 765
Hin1II CATG 8 cut(s) 116, 506, 542, 889, 917, 1018, 1687, 1696
Hin6I GCGC 2 cut(s) 75, 110
HinP1I GCGC 2 cut(s) 75, 110
HincII GTYRAC 2 cut(s) 982, 1342
HindII GTYRAC 2 cut(s) 982, 1342
HinfI GANTC 9 cut(s) 391, 481, 668, 828, 931, 1064, 1235, 1244, 1416
HpaI GTTAAC 1 cut(s) 1342
HpaII CCGG 3 cut(s) 56, 284, 1665
HphI GGTGA 5 cut(s) 523, 964, 1465, 1702, 1728
Hpy166II GTNNAC 4 cut(s) 214, 352, 982, 1342
Hpy8I GTNNAC 4 cut(s) 214, 352, 982, 1342
Hpy99I CGWCG 2 cut(s) 74, 1324
HpyAV CCTTC 5 cut(s) 419, 1182, 1259, 1439, 1723
HpyCH4III ACNGT 5 cut(s) 535, 655, 750, 1207, 1671
HpyCH4IV ACGT 4 cut(s) 300, 874, 1322, 1392
HpyCH4V TGCA 8 cut(s) 331, 538, 684, 745, 1014, 1022, 1072, 1618
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 133, 1283, 1358, 1624, 1730, 1739
HpyF3I CTNAG 2 cut(s) 863, 1248
HpySE526I ACGT 4 cut(s) 300, 874, 1322, 1392
Hsp92I GRCGYC 3 cut(s) 10, 60, 765
Hsp92II CATG 8 cut(s) 116, 506, 542, 889, 917, 1018, 1687, 1696
HspAI GCGC 2 cut(s) 75, 110
Kpn2I TCCGGA 1 cut(s) 283
KspAI GTTAAC 1 cut(s) 1342
LguI GCTCTTC 1 cut(s) 1650
Lsp1109I GCAGC 5 cut(s) 153, 1264, 1336, 1647, 1711
LweI GCATC 6 cut(s) 76, 167, 1141, 1435, 1469, 1742
MabI ACCWGGT 1 cut(s) 1364
MaeI CTAG 4 cut(s) 239, 561, 993, 1053
MaeII ACGT 4 cut(s) 300, 874, 1322, 1392
MaeIII GTNAC 5 cut(s) 434, 451, 1568, 1583, 1679
MbiI CCGCTC 1 cut(s) 1708
MboII GAAGA 7 cut(s) 235, 881, 891, 940, 1460, 1534, 1667
MflI RGATCY 3 cut(s) 610, 1048, 1524
MhlI GDGCHC 2 cut(s) 50, 595
MluI ACGCGT 1 cut(s) 1580
MlyI GAGTC 5 cut(s) 385, 475, 822, 940, 1073
MmeI TCCRAC 3 cut(s) 1237, 1404, 1471
MroI TCCGGA 1 cut(s) 283
MseI TTAA 7 cut(s) 278, 579, 638, 710, 1008, 1341, 1553
MslI CAYNNNNRTG 2 cut(s) 84, 1604
MspA1I CMGCKG 3 cut(s) 588, 772, 1724
MspI CCGG 3 cut(s) 56, 284, 1665
MspR9I CCNGG 4 cut(s) 721, 737, 1366, 1665
MvaI CCWGG 3 cut(s) 721, 737, 1366
MvnI CGCG 2 cut(s) 1299, 1582
MwoI GCNNNNNNNGC 7 cut(s) 116, 133, 1283, 1358, 1624, 1730, 1739
NciI CCSGG 1 cut(s) 1665
NcoI CCATGG 1 cut(s) 1692
NlaIII CATG 8 cut(s) 116, 506, 542, 889, 917, 1018, 1687, 1696
NlaIV GGNNCC 2 cut(s) 49, 1370
NmeAIII GCCGAG 2 cut(s) 88, 568
NmuCI GTSAC 4 cut(s) 434, 451, 1568, 1583
NspI RCATGY 3 cut(s) 542, 889, 1687
PaeI GCATGC 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 913
PciI ACATGT 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 1650
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PfeI GAWTC 4 cut(s) 668, 1235, 1244, 1416
PflFI GACNNNGTC 1 cut(s) 1589
PkrI GCNGC 8 cut(s) 40, 168, 1279, 1351, 1662, 1707, 1723, 1726
PleI GAGTC 5 cut(s) 385, 475, 822, 939, 1072
PpsI GAGTC 5 cut(s) 385, 475, 822, 939, 1072
PpuMI RGGWCCY 1 cut(s) 1368
PscI ACATGT 1 cut(s) 885
PsiI TTATAA 1 cut(s) 1406
Psp1406I AACGTT 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 1368
Psp6I CCWGG 3 cut(s) 719, 735, 1364
PspGI CCWGG 3 cut(s) 719, 735, 1364
PspN4I GGNNCC 2 cut(s) 49, 1370
PspPI GGNCC 3 cut(s) 1130, 1331, 1368
PspPPI RGGWCCY 1 cut(s) 1368
PspXI VCTCGAGB 1 cut(s) 140
PsuI RGATCY 3 cut(s) 610, 1048, 1524
PsyI GACNNNGTC 1 cut(s) 1589
RsaI GTAC 2 cut(s) 381, 1388
RsaNI GTAC 2 cut(s) 380, 1387
RseI CAYNNNNRTG 2 cut(s) 84, 1604
SapI GCTCTTC 1 cut(s) 1650
SaqAI TTAA 7 cut(s) 278, 579, 638, 710, 1008, 1341, 1553
SatI GCNGC 8 cut(s) 39, 167, 1278, 1350, 1661, 1706, 1722, 1725
Sau96I GGNCC 3 cut(s) 1130, 1331, 1368
ScaI AGTACT 1 cut(s) 1388
SchI GAGTC 5 cut(s) 385, 475, 822, 940, 1073
ScrFI CCNGG 4 cut(s) 721, 737, 1366, 1665
SduI GDGCHC 2 cut(s) 50, 595
SexAI ACCWGGT 1 cut(s) 1364
SfaNI GCATC 6 cut(s) 76, 167, 1141, 1435, 1469, 1742
SfcI CTRYAG 2 cut(s) 570, 1563
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 2 cut(s) 1130, 1368
SlaI CTCGAG 1 cut(s) 140
SmiI ATTTAAAT 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 84, 1604
SmlI CTYRAG 3 cut(s) 22, 140, 218
SmoI CTYRAG 3 cut(s) 22, 140, 218
SphI GCATGC 1 cut(s) 542
SsiI CCGC 9 cut(s) 39, 125, 586, 770, 908, 1092, 1505, 1706, 1722
SspMI CTAG 4 cut(s) 239, 561, 993, 1053
StyD4I CCNGG 4 cut(s) 719, 735, 1364, 1663
StyI CCWWGG 1 cut(s) 1692
SwaI ATTTAAAT 1 cut(s) 279
TaaI ACNGT 5 cut(s) 535, 655, 750, 1207, 1671
TaiI ACGT 4 cut(s) 303, 877, 1325, 1395
TaqI TCGA 3 cut(s) 141, 321, 466
TatI WGTACW 1 cut(s) 1386
TauI GCSGC 3 cut(s) 41, 1708, 1724
TfiI GAWTC 4 cut(s) 668, 1235, 1244, 1416
Tru1I TTAA 7 cut(s) 278, 579, 638, 710, 1008, 1341, 1553
Tru9I TTAA 7 cut(s) 278, 579, 638, 710, 1008, 1341, 1553
TscAI CASTG 2 cut(s) 1341, 1429
TseFI GTSAC 4 cut(s) 434, 451, 1568, 1583
TseI GCWGC 5 cut(s) 166, 1277, 1349, 1660, 1724
Tsp45I GTSAC 4 cut(s) 434, 451, 1568, 1583
TspDTI ATGAA 3 cut(s) 930, 1535, 1588
TspGWI ACGGA 1 cut(s) 1424
TspRI CASTG 2 cut(s) 1341, 1429
Tth111I GACNNNGTC 1 cut(s) 1589
VpaK11BI GGWCC 2 cut(s) 1130, 1368
XapI RAATTY 5 cut(s) 95, 334, 397, 581, 1099
XceI RCATGY 3 cut(s) 542, 889, 1687
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 2 cut(s) 213, 351
XspI CTAG 4 cut(s) 239, 561, 993, 1053
ZrmI AGTACT 1 cut(s) 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.