Rorug02G0648400

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000862
Physical Location & Seq
Reverse (-)
75776771 .. 75779173
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0648400.1

Sequence Viewer

Length: 2403 bp
ATGGATGAGGCTACCAACACCCCTAAGGCATTAAAGACTCCTAAGTCTTTAGGGACCCCCAAGGGCAATGTGACGGATCCATTCGAAAATCAAAAGGAAGATTCCGAGGAGAACAAAAAGCAGGGACAAGAGAATGCTGATCAGAAGGAGGAAGAGAAGGACAGTACCGGTGTCGAACACAGCGGTCCCCTTGAGCCGGTTGAGATACCGGAAGATCCCCATCCCACCCTCGAAAAGCTTTGTGATGACATTGAGCGATGCATCTCTTCATTGCCTACACCTAACGAGAAAGATGAAAACCAACAAGAAGATAAGGATAATGCAAATGAGGCAAAAGATACAAAAGGTGATGAGAAGGATGAAAAGGATACAAAAGATGACGAGGAAGAAAAGGATGTAAAAGATGACAAGGAAGAAGAGAAGGGTGAGAAAGATGCAAAGGAAGAAAAGGATGTGAAAGATGAGAATAAGGATGATGAAAAGGCTGCAGATCAGGATGAGAAAGTACTAGGTGACAAAGATGCAAAGGATGAGAAAGAGGCTGAAAAAGTCGTCGAAAAAGAAAAGGATCTCCTTCCAGACGAGCTCCTTAAGCTTATTGACAAGTTCTTGACTTGGGTAGATGATATCTGTTGCGAAGGCAGATGCAGGTGGGGAACGGTGCCTCAAGACGACGAGGCGTTCTTGGAGGCTGTGGATCTGATGGCCAAGATGAGGGAGTCTCTCTTGTCCGTCTGTTCTATGGGATTGAAATTCCAAGAAAGGCACGGCGGTTTGATCAACCGCCTAAGTAGCATTCAGCACCGAGCCATGGCCTATCTGGAAGAAGAATTCAGAATCCTTCTAGAAGAATCCAAAACCGAGTCTGATCATCATCATCACTCTACAGCAGCTGATCACAACCATAACCATAACCATAACAGTAACAGTAACAAAGACAGCAGCAGTGGTAACAAAGGAAAGAACGATCAAGAATCACAGCCGGAATCCGAGTCAGAAGGAGTTGTTGAATTTCCCGGGTTTCCTCAGGAGGTTGTGTCCAACCTGAATAAAATAGCCAACAAGATGATCTCCGCCGGCTACGAATCCGAGTGTTGCGAGGTCTACATCATCTCCAGGAGGCATGCATTCGATGAAAATCTCCACAAGCTCGGCCTAGAGAAGCACAGCATCGACGACGTGCAGAAGATGCACTGGGAAGCGCTGGAGAGAGATATCATGTTGTGGGTCAAGGCATTCAAGCAATGCTCGCAATTCTACTTTTCCGGTGAGCGCCAGCTGGCAGAGTCGGTGTTTCCCGAGCCGGTGCTTTCCGAGCAGACGCCGATATGGTCGTGTCTGTTCAGCAATCTGAGCAGAGGAGTGATGATACAGCTCCTGAATTTCGCCGAAGGAATCGCCATGTCGAAAAGAGCGGCGGAGAAACTGTTCAAGACGCTCGATATGTACGAGGCGGTCAAGGAGATGCTCCCCAAGATAGACGAGCTTTTTCCGGAGGAGTGCGCCAGGGAGCTGAAGACGGAGACCACGACGGTGCTGAACCGGCTGGGCGAGGCGGCGATATTAATCTTCTGTGATCTGGAGAATTCCATCAAGGCCGAAACGGGTCGGAACCCGGTTCCGGGCGGGGCGGTCCACCCGTTGACCCGGTACACGATGAACTACCTGAGATACGCGTGCGAGTACAAGGAGACGCTGGAGCTGGTGTTCAAGGAGCACACCAAGATCGAGCGGTCCGACTCGACGAGCAGGCCTGACGGTCGCGACGATTACGACGCGAGCGACACGTCGTTCGAGATACCCGACGACGAGTCGCCGTTCGCGGTGCAACTGGCGCGGGTGATGGACTTGTTGGACTCGAACCTGGAGGGGAAGGCGAAGCTGTACAAGGACGTGGCGTTGAGCTCGATTTTCATGATGAACAACGGGCGCTACATCTTGCAGAAGATCAAGGGCTCTCCGGAGATCAACGCCTGCATGGGCGACAAGTGGTGCCGGAAGAGGTCGACGGAGCTGAGGCAGTACCACAAGAACTACCAGAGAGAGACGTGGAGCCGGCTGTTGAGTTTTTTGAGCCACGAGGGGCTGAGCAACCACGGCAAAGTGAGCAAGCCTGTGCTGAAAGAGAGGTTCAAGAGCTTCAACGCTATGTTTGATGAGATTCATAAGACGCAGAGCACGTGGGTGGTGAGTGACGAGCAGCTTCAGTCGGAGCTTAGGGTTTCGATATCGGCGGTGGTCATTCCGGCGTACCGGTCGTTCTTGGGGAGATTCTCGCAGACGTTGGACCCGGGAAGGCAAACAGAGAAGTACATTAAGTTTCAGCCGGAAGATATAGAGACGTACATTGATGAGCTATTTGATGGCAACCCCAGTACCTCCATATTTAGGAAAAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

800

Amino Acids

91.59

Weight (kDa)

4.89

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_N PF20669 224 - 284 8.6e-06 Exocyst complex component Exo70 N-terminal
Exo70_C PF03081 409 - 785 9.4e-126 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 639
AasI GACNNNNNNGTC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 639
AccB1I GGYRCC 2 cut(s) 661, 1992
AccBSI CCGCTC 2 cut(s) 1415, 1732
AccI GTMKAC 2 cut(s) 1104, 2006
AccII CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
AccIII TCCGGA 2 cut(s) 1492, 1960
AclWI GGATC 5 cut(s) 71, 84, 209, 576, 705
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 5 cut(s) 752, 830, 1010, 1381, 1585
AcuI CTGAAG 2 cut(s) 1535, 2189
AcvI CACGTG 1 cut(s) 2181
AcyI GRCGYC 1 cut(s) 1322
AfeI AGCGCT 1 cut(s) 1203
AfiI CCNNNNNNNGG 6 cut(s) 196, 714, 811, 1621, 1622, 2388
AflII CTTAAG 1 cut(s) 590
AflIII ACRYGT 2 cut(s) 1674, 1785
AgeI ACCGGT 2 cut(s) 167, 2253
AgsI TTSAA 7 cut(s) 751, 1010, 1240, 1432, 1711, 2134, 2143
AhdI GACNNNNNGTC 1 cut(s) 1810
AjiI CACGTC 4 cut(s) 1180, 1788, 1894, 2049
AjnI CCWGG 3 cut(s) 1115, 1505, 1863
AleI CACNNNNGTG 2 cut(s) 1532, 2183
AloI GAACNNNNNNTCC 4 cut(s) 1412, 1444, 1691, 1723
Alw21I GWGCWC 4 cut(s) 588, 1719, 1907, 2180
Alw26I GTCTC 5 cut(s) 726, 1517, 1685, 2039, 2333
AlwI GGATC 5 cut(s) 71, 84, 209, 576, 705
AlwNI CAGNNNCTG 2 cut(s) 893, 1378
Ama87I CYCGRG 3 cut(s) 1016, 1298, 2290
Aor13HI TCCGGA 2 cut(s) 1492, 1960
Aor51HI AGCGCT 1 cut(s) 1203
AoxI GGCC 5 cut(s) 705, 813, 1153, 1596, 1751
ApeKI GCWGC 4 cut(s) 485, 890, 942, 2200
ApoI RAATTY 5 cut(s) 752, 830, 1010, 1381, 1585
ArsI GACNNNNNNTTYG 2 cut(s) 1775, 1807
AseI ATTAAT 1 cut(s) 1565
AsiGI ACCGGT 2 cut(s) 167, 2253
Asp700I GAANNNNTTC 1 cut(s) 1427
AspLEI GCGC 5 cut(s) 1204, 1275, 1505, 1837, 1932
AspS9I GGNCC 5 cut(s) 54, 185, 1633, 1734, 2287
AsuC2I CCSGG 7 cut(s) 1017, 1018, 1616, 1623, 1648, 2291, 2292
AsuHPI GGTGA 6 cut(s) 359, 437, 524, 1280, 1852, 2200
AsuII TTCGAA 1 cut(s) 84
AvaI CYCGRG 3 cut(s) 1016, 1298, 2290
AvaII GGWCC 5 cut(s) 54, 185, 1633, 1734, 2287
AxyI CCTNAGG 2 cut(s) 24, 1026
BaeI ACNNNNGTAYC 2 cut(s) 2359, 2392
BalI TGGCCA 1 cut(s) 707
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 661, 1992
BanII GRGCYC 3 cut(s) 588, 1907, 1958
BauI CACGAG 1 cut(s) 2078
BbrPI CACGTG 1 cut(s) 2181
BbsI GAAGAC 1 cut(s) 1523
Bbv12I GWGCWC 4 cut(s) 588, 1719, 1907, 2180
BbvCI CCTCAGC 1 cut(s) 2015
BbvI GCAGC 4 cut(s) 472, 902, 954, 2212
BccI CCATC 5 cut(s) 228, 697, 1598, 1837, 2357
BceAI ACGGC 3 cut(s) 784, 1801, 2113
BciT130I CCWGG 3 cut(s) 1117, 1507, 1865
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 4 cut(s) 139, 777, 868, 895
BcnI CCSGG 7 cut(s) 1017, 1018, 1616, 1623, 1648, 2291, 2292
BcoDI GTCTC 5 cut(s) 726, 1517, 1685, 2039, 2333
BfaI CTAG 3 cut(s) 509, 845, 1157
BfmI CTRYAG 2 cut(s) 486, 885
BfoI RGCGCY 3 cut(s) 1205, 1276, 1933
BfrI CTTAAG 1 cut(s) 590
BfuAI ACCTGC 1 cut(s) 639
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 6 cut(s) 486, 891, 943, 1416, 1557, 2201
BlpI GCTNAGC 1 cut(s) 2087
BlsI GCNGC 6 cut(s) 487, 892, 944, 1417, 1558, 2202
BmcAI AGTACT 1 cut(s) 507
Bme18I GGWCC 5 cut(s) 54, 185, 1633, 1734, 2287
BmeRI GACNNNNNGTC 1 cut(s) 1810
BmeT110I CYCGRG 3 cut(s) 1016, 1298, 2290
BmgBI CACGTC 4 cut(s) 1180, 1788, 1894, 2049
BmgT120I GGNCC 5 cut(s) 54, 185, 1633, 1734, 2287
BmrI ACTGGG 2 cut(s) 1204, 2367
BmsI GCATC 8 cut(s) 248, 270, 424, 511, 635, 1179, 1179, 1455
BmuI ACTGGG 2 cut(s) 1204, 2367
BpiI GAAGAC 1 cut(s) 1523
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 5 cut(s) 1099, 1226, 1601, 1718, 1886
Bpu10I CCTNAGC 2 cut(s) 2015, 2216
Bpu1102I GCTNAGC 1 cut(s) 2087
Bpu14I TTCGAA 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 212, 651
BpuMI CCSGG 7 cut(s) 1017, 1018, 1616, 1623, 1648, 2291, 2292
BsaAI YACGTR 1 cut(s) 2181
BsaBI GATNNNNATC 4 cut(s) 219, 873, 1137, 1565
BsaHI GRCGYC 1 cut(s) 1322
BsaI GGTCTC 1 cut(s) 1517
BsaJI CCNNGG 7 cut(s) 60, 105, 810, 1016, 1506, 2095, 2290
BsaWI WCCGGW 6 cut(s) 167, 208, 1265, 1492, 1960, 2253
BsaXI ACNNNNNCTCC 8 cut(s) 1022, 1052, 1097, 1127, 1412, 1442, 1691, 1721
Bsc4I CCNNNNNNNGG 6 cut(s) 196, 714, 811, 1621, 1622, 2388
Bse118I RCCGGY 7 cut(s) 167, 196, 1076, 1303, 1542, 2055, 2253
Bse1I ACTGG 3 cut(s) 1199, 1836, 2373
Bse21I CCTNAGG 2 cut(s) 24, 1026
Bse3DI GCAATG 3 cut(s) 73, 269, 1250
Bse8I GATNNNNATC 4 cut(s) 219, 873, 1137, 1565
BseAI TCCGGA 2 cut(s) 1492, 1960
BseBI CCWGG 3 cut(s) 1117, 1507, 1865
BseDI CCNNGG 7 cut(s) 60, 105, 810, 1016, 1506, 2095, 2290
BseGI GGATG 8 cut(s) 10, 220, 364, 400, 457, 478, 502, 535
BseJI GATNNNNATC 4 cut(s) 219, 873, 1137, 1565
BseLI CCNNNNNNNGG 6 cut(s) 196, 714, 811, 1621, 1622, 2388
BseMI GCAATG 3 cut(s) 73, 269, 1250
BseMII CTCAG 5 cut(s) 1040, 1343, 1658, 2006, 2078
BseNI ACTGG 3 cut(s) 1199, 1836, 2373
BseRI GAGGAG 3 cut(s) 122, 1374, 1511
BseXI GCAGC 4 cut(s) 472, 902, 954, 2212
BseYI CCCAGC 1 cut(s) 1546
BsgI GTGCAG 1 cut(s) 1202
Bsh1236I CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
Bsh1285I CGRYCG 2 cut(s) 1762, 2258
BshFI GGCC 5 cut(s) 707, 815, 1155, 1598, 1753
BshNI GGYRCC 2 cut(s) 661, 1992
BshTI ACCGGT 2 cut(s) 167, 2253
BsiEI CGRYCG 2 cut(s) 1762, 2258
BsiHKAI GWGCWC 4 cut(s) 588, 1719, 1907, 2180
BsiHKCI CYCGRG 3 cut(s) 1016, 1298, 2290
BslFI GGGAC 3 cut(s) 67, 138, 171
BslI CCNNNNNNNGG 6 cut(s) 196, 714, 811, 1621, 1622, 2388
BsmAI GTCTC 5 cut(s) 726, 1517, 1685, 2039, 2333
BsmBI CGTCTC 3 cut(s) 1685, 2039, 2333
BsmFI GGGAC 3 cut(s) 67, 138, 171
BsmI GAATGC 4 cut(s) 139, 795, 1127, 1235
BsnI GGCC 5 cut(s) 707, 815, 1155, 1598, 1753
Bso31I GGTCTC 1 cut(s) 1517
BsoBI CYCGRG 3 cut(s) 1016, 1298, 2290
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 5 cut(s) 588, 1719, 1907, 1958, 2180
Bsp13I TCCGGA 2 cut(s) 1492, 1960
Bsp1407I TGTACA 1 cut(s) 1884
Bsp1720I GCTNAGC 1 cut(s) 2087
Bsp19I CCATGG 1 cut(s) 810
Bsp68I TCGCGA 1 cut(s) 1764
BspANI GGCC 5 cut(s) 707, 815, 1155, 1598, 1753
BspCNI CTCAG 5 cut(s) 1039, 1344, 1659, 2007, 2079
BspEI TCCGGA 2 cut(s) 1492, 1960
BspFNI CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
BspHI TCATGA 1 cut(s) 1914
BspMAI CTGCAG 1 cut(s) 490
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 5 cut(s) 71, 84, 209, 576, 705
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 2 cut(s) 661, 1992
BspTI CTTAAG 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 1517
BsrBI CCGCTC 2 cut(s) 1415, 1732
BsrDI GCAATG 3 cut(s) 73, 269, 1250
BsrFI RCCGGY 7 cut(s) 167, 196, 1076, 1303, 1542, 2055, 2253
BsrGI TGTACA 1 cut(s) 1884
BsrI ACTGG 3 cut(s) 1199, 1836, 2373
BssAI RCCGGY 7 cut(s) 167, 196, 1076, 1303, 1542, 2055, 2253
BssECI CCNNGG 7 cut(s) 60, 105, 810, 1016, 1506, 2095, 2290
BssNI GRCGYC 1 cut(s) 1322
BssSI CACGAG 1 cut(s) 2078
BssT1I CCWWGG 2 cut(s) 60, 810
Bst2BI CACGAG 1 cut(s) 2078
Bst2UI CCWGG 3 cut(s) 1117, 1507, 1865
Bst4CI ACNGT 7 cut(s) 164, 661, 923, 929, 1428, 1534, 1760
Bst6I CTCTTC 4 cut(s) 147, 271, 411, 1994
BstACI GRCGYC 1 cut(s) 1322
BstAFI CTTAAG 1 cut(s) 590
BstAPI GCANNNNNTGC 1 cut(s) 1189
BstAUI TGTACA 1 cut(s) 1884
BstBAI YACGTR 1 cut(s) 2181
BstBI TTCGAA 1 cut(s) 84
BstDEI CTNAG 9 cut(s) 24, 42, 788, 1026, 1352, 1667, 2015, 2087, 2216
BstDSI CCRYGG 2 cut(s) 810, 2095
BstF5I GGATG 8 cut(s) 10, 220, 364, 400, 457, 478, 502, 535
BstFNI CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
BstH2I RGCGCY 3 cut(s) 1205, 1276, 1933
BstHHI GCGC 5 cut(s) 1204, 1275, 1505, 1837, 1932
BstMAI GTCTC 5 cut(s) 726, 1517, 1685, 2039, 2333
BstMCI CGRYCG 2 cut(s) 1762, 2258
BstNI CCWGG 3 cut(s) 1117, 1507, 1865
BstNSI RCATGY 1 cut(s) 1127
BstSFI CTRYAG 2 cut(s) 486, 885
BstUI CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
BstV1I GCAGC 4 cut(s) 472, 902, 954, 2212
BstV2I GAAGAC 1 cut(s) 1523
BstX2I RGATCY 4 cut(s) 76, 214, 568, 697
BstYI RGATCY 4 cut(s) 76, 214, 568, 697
Bsu36I CCTNAGG 2 cut(s) 24, 1026
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 5 cut(s) 707, 815, 1155, 1598, 1753
BtgI CCRYGG 2 cut(s) 810, 2095
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 4 cut(s) 1180, 1788, 1894, 2049
BtsCI GGATG 8 cut(s) 10, 220, 364, 400, 457, 478, 502, 535
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 2 cut(s) 952, 1192
BtuMI TCGCGA 1 cut(s) 1764
BveI ACCTGC 1 cut(s) 639
CaiI CAGNNNCTG 2 cut(s) 893, 1378
CciI TCATGA 1 cut(s) 1914
CfoI GCGC 5 cut(s) 1204, 1275, 1505, 1837, 1932
Cfr10I RCCGGY 7 cut(s) 167, 196, 1076, 1303, 1542, 2055, 2253
Cfr13I GGNCC 5 cut(s) 54, 185, 1633, 1734, 2287
Cfr9I CCCGGG 2 cut(s) 1016, 2290
CpoI CGGWCCG 1 cut(s) 1734
CseI GACGC 5 cut(s) 1330, 1444, 1702, 1784, 2179
CspAI ACCGGT 2 cut(s) 167, 2253
CspI CGGWCCG 1 cut(s) 1734
CviAII CATG 6 cut(s) 811, 1124, 1219, 1402, 1915, 1978
DdeI CTNAG 9 cut(s) 24, 42, 788, 1026, 1352, 1667, 2015, 2087, 2216
DrdI GACNNNNNNGTC 1 cut(s) 43
DriI GACNNNNNGTC 1 cut(s) 1810
DseDI GACNNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 4 cut(s) 147, 271, 411, 1994
Eam1105I GACNNNNNGTC 1 cut(s) 1810
EarI CTCTTC 4 cut(s) 147, 271, 411, 1994
EciI GGCGGA 2 cut(s) 1063, 1433
Ecl136II GAGCTC 2 cut(s) 586, 1905
Eco130I CCWWGG 2 cut(s) 60, 810
Eco147I AGGCCT 1 cut(s) 1753
Eco24I GRGCYC 3 cut(s) 588, 1907, 1958
Eco31I GGTCTC 1 cut(s) 1517
Eco32I GATATC 3 cut(s) 628, 1216, 2229
Eco47I GGWCC 5 cut(s) 54, 185, 1633, 1734, 2287
Eco47III AGCGCT 1 cut(s) 1203
Eco53kI GAGCTC 2 cut(s) 586, 1905
Eco57I CTGAAG 2 cut(s) 1535, 2189
Eco72I CACGTG 1 cut(s) 2181
Eco81I CCTNAGG 2 cut(s) 24, 1026
Eco88I CYCGRG 3 cut(s) 1016, 1298, 2290
EcoICRI GAGCTC 2 cut(s) 586, 1905
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 2 cut(s) 830, 1585
EcoRII CCWGG 3 cut(s) 1115, 1505, 1863
EcoRV GATATC 3 cut(s) 628, 1216, 2229
EcoT14I CCWWGG 2 cut(s) 60, 810
EcoT22I ATGCAT 2 cut(s) 263, 1129
EcoT38I GRGCYC 3 cut(s) 588, 1907, 1958
ErhI CCWWGG 2 cut(s) 60, 810
Esp3I CGTCTC 3 cut(s) 1685, 2039, 2333
FaeI CATG 6 cut(s) 814, 1127, 1222, 1405, 1918, 1981
FaqI GGGAC 3 cut(s) 67, 138, 171
FatI CATG 6 cut(s) 810, 1123, 1218, 1401, 1914, 1977
FauI CCCGC 2 cut(s) 1619, 1830
FbaI TGATCA 4 cut(s) 139, 777, 868, 895
FblI GTMKAC 2 cut(s) 1104, 2006
Fnu4HI GCNGC 6 cut(s) 486, 891, 943, 1416, 1557, 2201
FokI GGATG 8 cut(s) 17, 207, 371, 407, 464, 485, 509, 542
FriOI GRGCYC 3 cut(s) 588, 1907, 1958
Fsp4HI GCNGC 6 cut(s) 486, 891, 943, 1416, 1557, 2201
FspBI CTAG 3 cut(s) 509, 845, 1157
GlaI GCGC 5 cut(s) 1203, 1274, 1504, 1836, 1931
GluI GCNGC 6 cut(s) 486, 891, 943, 1416, 1557, 2201
GsaI CCCAGC 1 cut(s) 1550
GsuI CTGGAG 5 cut(s) 1099, 1226, 1601, 1718, 1886
HaeII RGCGCY 3 cut(s) 1205, 1276, 1933
HaeIII GGCC 5 cut(s) 707, 815, 1155, 1598, 1753
HgaI GACGC 5 cut(s) 1330, 1444, 1702, 1784, 2179
HhaI GCGC 5 cut(s) 1204, 1275, 1505, 1837, 1932
Hin1I GRCGYC 1 cut(s) 1322
Hin1II CATG 6 cut(s) 814, 1127, 1222, 1405, 1918, 1981
Hin6I GCGC 5 cut(s) 1202, 1273, 1503, 1835, 1930
HinP1I GCGC 5 cut(s) 1202, 1273, 1503, 1835, 1930
HincII GTYRAC 2 cut(s) 1644, 2007
HindII GTYRAC 2 cut(s) 1644, 2007
HindIII AAGCTT 2 cut(s) 236, 593
HphI GGTGA 6 cut(s) 359, 437, 524, 1280, 1852, 2200
Hpy166II GTNNAC 5 cut(s) 1105, 1636, 1644, 1653, 2007
Hpy8I GTNNAC 5 cut(s) 1105, 1636, 1644, 1653, 2007
HpyCH4III ACNGT 7 cut(s) 164, 661, 923, 929, 1428, 1534, 1760
HpyCH4IV ACGT 7 cut(s) 1179, 1787, 1893, 2048, 2180, 2282, 2342
HpyF3I CTNAG 9 cut(s) 24, 42, 788, 1026, 1352, 1667, 2015, 2087, 2216
HpySE526I ACGT 7 cut(s) 1179, 1787, 1893, 2048, 2180, 2282, 2342
Hsp92I GRCGYC 1 cut(s) 1322
Hsp92II CATG 6 cut(s) 814, 1127, 1222, 1405, 1918, 1981
HspAI GCGC 5 cut(s) 1202, 1273, 1503, 1835, 1930
KflI GGGWCCC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 1492, 1960
KroI GCCGGC 2 cut(s) 1076, 2055
KroNI GCCGGC 2 cut(s) 1078, 2057
Ksp22I TGATCA 4 cut(s) 139, 777, 868, 895
LmnI GCTCC 9 cut(s) 591, 1380, 1473, 1510, 1699, 1714, 2011, 2052, 2212
Lsp1109I GCAGC 4 cut(s) 472, 902, 954, 2212
LweI GCATC 8 cut(s) 248, 270, 424, 511, 635, 1179, 1179, 1455
MaeI CTAG 3 cut(s) 509, 845, 1157
MaeII ACGT 7 cut(s) 1179, 1787, 1893, 2048, 2180, 2282, 2342
MaeIII GTNAC 6 cut(s) 70, 512, 923, 929, 950, 2192
MbiI CCGCTC 2 cut(s) 1415, 1732
MflI RGATCY 4 cut(s) 76, 214, 568, 697
MhlI GDGCHC 5 cut(s) 588, 1719, 1907, 1958, 2180
MlsI TGGCCA 1 cut(s) 707
MluCI AATT 6 cut(s) 752, 830, 1010, 1253, 1381, 1585
MluI ACGCGT 1 cut(s) 1674
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 8 cut(s) 31, 728, 872, 1001, 1295, 1733, 1820, 1850
MmeI TCCRAC 6 cut(s) 1065, 1589, 1761, 1833, 2190, 2265
Mox20I TGGCCA 1 cut(s) 707
Mph1103I ATGCAT 2 cut(s) 263, 1129
MroI TCCGGA 2 cut(s) 1492, 1960
MroNI GCCGGC 2 cut(s) 1076, 2055
MroXI GAANNNNTTC 1 cut(s) 1427
MscI TGGCCA 1 cut(s) 707
MseI TTAA 4 cut(s) 32, 591, 1565, 2316
MslI CAYNNNNRTG 2 cut(s) 1532, 2183
Msp20I TGGCCA 1 cut(s) 707
MspA1I CMGCKG 3 cut(s) 183, 893, 1279
MspCI CTTAAG 1 cut(s) 590
Mva1269I GAATGC 4 cut(s) 139, 795, 1127, 1235
MvaI CCWGG 3 cut(s) 1117, 1507, 1865
MvnI CGCG 5 cut(s) 1676, 1764, 1778, 1823, 1837
NaeI GCCGGC 2 cut(s) 1078, 2057
NciI CCSGG 7 cut(s) 1017, 1018, 1616, 1623, 1648, 2291, 2292
NcoI CCATGG 1 cut(s) 810
NgoMIV GCCGGC 2 cut(s) 1076, 2055
NlaIII CATG 6 cut(s) 814, 1127, 1222, 1405, 1918, 1981
NmeAIII GCCGAG 1 cut(s) 1131
NmuCI GTSAC 3 cut(s) 70, 512, 2192
NruI TCGCGA 1 cut(s) 1764
NsiI ATGCAT 2 cut(s) 263, 1129
NspI RCATGY 1 cut(s) 1127
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 1532, 2183
PaeI GCATGC 1 cut(s) 1127
PagI TCATGA 1 cut(s) 1914
PaqCI CACCTGC 1 cut(s) 639
PceI AGGCCT 1 cut(s) 1753
PcsI WCGNNNNNNNCGW 7 cut(s) 1446, 1526, 1734, 1764, 1779, 1800, 2177
PctI GAATGC 4 cut(s) 139, 795, 1127, 1235
PdiI GCCGGC 2 cut(s) 1078, 2057
PdmI GAANNNNTTC 1 cut(s) 1427
PfeI GAWTC 9 cut(s) 101, 837, 851, 974, 986, 1085, 1395, 2161, 2271
PfoI TCCNGGA 1 cut(s) 1115
PinAI ACCGGT 2 cut(s) 167, 2253
PkrI GCNGC 6 cut(s) 487, 892, 944, 1417, 1558, 2202
PleI GAGTC 8 cut(s) 31, 727, 871, 1000, 1294, 1733, 1819, 1850
PmaCI CACGTG 1 cut(s) 2181
PmlI CACGTG 1 cut(s) 2181
PpsI GAGTC 8 cut(s) 31, 727, 871, 1000, 1294, 1733, 1819, 1850
Ppu21I YACGTR 1 cut(s) 2181
PpuMI RGGWCCY 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 1565
Psp124BI GAGCTC 2 cut(s) 588, 1907
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 1115, 1505, 1863
PspCI CACGTG 1 cut(s) 2181
PspFI CCCAGC 1 cut(s) 1546
PspGI CCWGG 3 cut(s) 1115, 1505, 1863
PspPI GGNCC 5 cut(s) 54, 185, 1633, 1734, 2287
PspPPI RGGWCCY 1 cut(s) 54
PsrI GAACNNNNNNTAC 2 cut(s) 2243, 2275
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 2 cut(s) 893, 1378
PsuI RGATCY 4 cut(s) 76, 214, 568, 697
PvuII CAGCTG 2 cut(s) 893, 1279
RruI TCGCGA 1 cut(s) 1764
RseI CAYNNNNRTG 2 cut(s) 1532, 2183
Rsr2I CGGWCCG 1 cut(s) 1734
RsrII CGGWCCG 1 cut(s) 1734
SacI GAGCTC 2 cut(s) 588, 1907
SalI GTCGAC 1 cut(s) 2005
SaqAI TTAA 4 cut(s) 32, 591, 1565, 2316
SatI GCNGC 6 cut(s) 486, 891, 943, 1416, 1557, 2201
Sau96I GGNCC 5 cut(s) 54, 185, 1633, 1734, 2287
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 8 cut(s) 31, 728, 872, 1001, 1295, 1733, 1820, 1850
SduI GDGCHC 5 cut(s) 588, 1719, 1907, 1958, 2180
SfaNI GCATC 8 cut(s) 248, 270, 424, 511, 635, 1179, 1179, 1455
SfcI CTRYAG 2 cut(s) 486, 885
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 5 cut(s) 54, 185, 1633, 1734, 2287
SmaI CCCGGG 2 cut(s) 1018, 2292
SmiMI CAYNNNNRTG 2 cut(s) 1532, 2183
SmlI CTYRAG 3 cut(s) 191, 590, 666
SmoI CTYRAG 3 cut(s) 191, 590, 666
SphI GCATGC 1 cut(s) 1127
Sse9I AATT 6 cut(s) 752, 830, 1010, 1253, 1381, 1585
SseBI AGGCCT 1 cut(s) 1753
SspMI CTAG 3 cut(s) 509, 845, 1157
SstI GAGCTC 2 cut(s) 588, 1907
StuI AGGCCT 1 cut(s) 1753
StyI CCWWGG 2 cut(s) 60, 810
TaaI ACNGT 7 cut(s) 164, 661, 923, 929, 1428, 1534, 1760
TaiI ACGT 7 cut(s) 1182, 1790, 1896, 2051, 2183, 2285, 2345
TasI AATT 6 cut(s) 752, 830, 1010, 1253, 1381, 1585
TatI WGTACW 4 cut(s) 505, 1683, 1884, 2310
TauI GCSGC 2 cut(s) 1418, 1559
TfiI GAWTC 9 cut(s) 101, 837, 851, 974, 986, 1085, 1395, 2161, 2271
Tru1I TTAA 4 cut(s) 32, 591, 1565, 2316
Tru9I TTAA 4 cut(s) 32, 591, 1565, 2316
TscAI CASTG 2 cut(s) 952, 1199
TseFI GTSAC 3 cut(s) 70, 512, 2192
TseI GCWGC 4 cut(s) 485, 890, 942, 2200
Tsp45I GTSAC 3 cut(s) 70, 512, 2192
TspDTI ATGAA 9 cut(s) 258, 309, 375, 492, 1149, 1673, 1903, 1934, 2153
TspGWI ACGGA 4 cut(s) 89, 721, 1535, 2024
TspMI CCCGGG 2 cut(s) 1016, 2290
TspRI CASTG 2 cut(s) 952, 1199
Vha464I CTTAAG 1 cut(s) 590
VpaK11BI GGWCC 5 cut(s) 54, 185, 1633, 1734, 2287
VspI ATTAAT 1 cut(s) 1565
XapI RAATTY 5 cut(s) 752, 830, 1010, 1381, 1585
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 1 cut(s) 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 817
XmaI CCCGGG 2 cut(s) 1016, 2290
XmiI GTMKAC 2 cut(s) 1104, 2006
XmnI GAANNNNTTC 1 cut(s) 1427
XspI CTAG 3 cut(s) 509, 845, 1157
ZrmI AGTACT 1 cut(s) 507
Zsp2I ATGCAT 2 cut(s) 263, 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.