pycom12g21250

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
22453598 .. 22457918
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21250.4

Sequence Viewer

Length: 1590 bp
ATGGGAGGATGCTTTTCGGACGTCAGGGGCGGCAAGGACGCCGTGGGCGGGGACCTGCCGAGGCCCACCGAACCGAACGCCAAGAACGACGGCGCTCACAATGATGCCATTGAATTATTTTACAGGGCGCATGGTGCTTACCCGCTTTTTACGCAACTCGAGTTATCCTTTTCAGCATCCAAGCTACTCAATCTTGACGTCACTTCAAAGAGTGATCCTATGGTGATTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTCGGGAGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGATGGTGCAGAATTTGATATTCCGTGTCTACGATGTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAACGATCAAGAGTTTCTTGGAGAAGGCAGTTGTGTCCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTTAACGTTGACTCTGAAAGGAAAAAATGGGCATGGAGGTTTGAGAAACTTTGGAACACTCACTGTGCATGCCGAGGAAACTATTGCTTCTAGGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTCTTTTCTAAAAGCGATCCTTTCTTAAGAATATCAAGGACTGTTGAGACTGGGGGCTCTATTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACAACCCACTGGTTATTGAGTGTTTTGATTTCAACAGCGATGGCAACCACATTCTTATTGGGAAACTCCAAAAATCAGTAGCAGACCTTGAAAAACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAGGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACGGGTTCAAATGGAGATCCTAGAAAACCCGAGTCTTTGCACTATATTGATCCGTATGGCCGGCTGAATTCTTACCAGCAGGCTATAACAGAAGTAGGACAAGTCATTCAGTTCTACGATGCAGATAAGAGTTTTCCTGCTTTGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCACACTGCTTCAACTTGAATGGAAATGCAAGTGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCTACGCAATGTTGCTCTCTCAGGACCGACGTTGTTTGGCCAAGTGGTTAACAGGGCTGCAGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAACAAATACTACGTCTTGCTCATTATAACAGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAATGCGGATTTCAAACAAATGGAGATCGATCTTTCCGGCAACACGCTGACAACGGGCAACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

530

Amino Acids

58.11

Weight (kDa)

5.43

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AatII GACGTC 2 cut(s) 24, 201
Acc36I ACCTGC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 231, 369
AciI CCGC 7 cut(s) 30, 48, 143, 604, 926, 1380, 1529
AclI AACGTT 1 cut(s) 494
AclWI GGATC 5 cut(s) 209, 641, 972, 1061, 1094
AcoI YGGCCR 2 cut(s) 1108, 1348
AcsI RAATTY 4 cut(s) 352, 415, 599, 1117
AcuI CTGAAG 2 cut(s) 297, 729
AcyI GRCGYC 3 cut(s) 21, 39, 198
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 48, 1193
AflII CTTAAG 1 cut(s) 655
AjnI CCWGG 2 cut(s) 737, 1382
AluBI AGCT 6 cut(s) 184, 256, 329, 955, 993, 1024
AluI AGCT 6 cut(s) 184, 256, 329, 955, 993, 1024
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 5 cut(s) 209, 641, 972, 1061, 1094
Ama87I CYCGRG 3 cut(s) 158, 266, 1079
AoxI GGCC 3 cut(s) 62, 1108, 1348
ApeKI GCWGC 2 cut(s) 1295, 1367
ApoI RAATTY 4 cut(s) 352, 415, 599, 1117
ArsI GACNNNNNNTTYG 4 cut(s) 400, 432, 1401, 1433
AspLEI GCGC 2 cut(s) 95, 130
AspS9I GGNCC 4 cut(s) 52, 63, 1334, 1386
AsuHPI GGTGA 3 cut(s) 235, 910, 1489
AvaI CYCGRG 3 cut(s) 158, 266, 1079
AvaII GGWCC 3 cut(s) 52, 1334, 1386
BaeGI GKGCMC 1 cut(s) 613
BalI TGGCCA 1 cut(s) 1350
BanII GRGCYC 1 cut(s) 689
BauI CACGAG 1 cut(s) 921
BbvI GCAGC 2 cut(s) 1282, 1354
BccI CCATC 5 cut(s) 242, 337, 815, 922, 1430
BceAI ACGGC 3 cut(s) 26, 106, 1227
BciT130I CCWGG 2 cut(s) 739, 1384
BciVI GTATCC 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 4 cut(s) 257, 579, 1011, 1071
BfmI CTRYAG 2 cut(s) 588, 1368
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 655
BfuAI ACCTGC 1 cut(s) 63
BfuI GTATCC 1 cut(s) 303
BglII AGATCT 1 cut(s) 628
BisI GCNGC 4 cut(s) 31, 1296, 1368, 1380
BlsI GCNGC 4 cut(s) 32, 1297, 1369, 1381
Bme1390I CCNGG 2 cut(s) 739, 1384
Bme18I GGWCC 3 cut(s) 52, 1334, 1386
BmeT110I CYCGRG 3 cut(s) 158, 266, 1079
BmgT120I GGNCC 4 cut(s) 52, 63, 1334, 1386
BmiI GGNNCC 2 cut(s) 53, 1388
BmrFI CCNGG 2 cut(s) 739, 1384
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 5 cut(s) 94, 185, 1159, 1459, 1493
BmuI ACTGGG 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 221
Bsa29I ATCGAT 1 cut(s) 1551
BsaHI GRCGYC 3 cut(s) 21, 39, 198
BsaJI CCNNGG 3 cut(s) 42, 59, 561
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 1193
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 2 cut(s) 685, 795
Bse3DI GCAATG 1 cut(s) 1324
BseBI CCWGG 2 cut(s) 739, 1384
BseCI ATCGAT 1 cut(s) 1551
BseDI CCNNGG 3 cut(s) 42, 59, 561
BseGI GGATG 2 cut(s) 14, 176
BseLI CCNNNNNNNGG 2 cut(s) 48, 1193
BseMI GCAATG 1 cut(s) 1324
BseMII CTCAG 3 cut(s) 746, 1257, 1344
BseNI ACTGG 2 cut(s) 685, 795
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 2 cut(s) 1282, 1354
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 3 cut(s) 64, 1110, 1350
BshVI ATCGAT 1 cut(s) 1551
BsiHKCI CYCGRG 3 cut(s) 158, 266, 1079
BsiSI CCGG 3 cut(s) 302, 1111, 1560
BslFI GGGAC 2 cut(s) 65, 1372
BslI CCNNNNNNNGG 2 cut(s) 48, 1193
BsmAI GTCTC 1 cut(s) 671
BsmFI GGGAC 2 cut(s) 65, 1372
BsnI GGCC 3 cut(s) 64, 1110, 1350
BsoBI CYCGRG 3 cut(s) 158, 266, 1079
Bsp1286I GDGCHC 2 cut(s) 613, 689
Bsp143I GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
BspACI CCGC 7 cut(s) 30, 48, 143, 604, 926, 1380, 1529
BspANI GGCC 3 cut(s) 64, 1110, 1350
BspCNI CTCAG 3 cut(s) 747, 1258, 1343
BspDI ATCGAT 1 cut(s) 1551
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 53, 1388
BspMAI CTGCAG 1 cut(s) 1372
BspMI ACCTGC 1 cut(s) 63
BspPI GGATC 5 cut(s) 209, 641, 972, 1061, 1094
BspTI CTTAAG 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 1324
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 2 cut(s) 685, 795
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 3 cut(s) 42, 59, 561
BssMI GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
BssNAI GTATAC 1 cut(s) 232
BssNI GRCGYC 3 cut(s) 21, 39, 198
BssSI CACGAG 1 cut(s) 921
Bst1107I GTATAC 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 921
Bst2UI CCWGG 2 cut(s) 739, 1384
Bst4CI ACNGT 4 cut(s) 553, 673, 768, 1225
BstACI GRCGYC 3 cut(s) 21, 39, 198
BstAFI CTTAAG 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstC8I GCNNGC 3 cut(s) 558, 1112, 1131
BstDEI CTNAG 3 cut(s) 755, 1266, 1330
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 14, 176
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 130
BstKTI GATC 9 cut(s) 217, 427, 631, 649, 967, 1069, 1102, 1551, 1555
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
BstMWI GCNNNNNNNGC 4 cut(s) 134, 151, 1301, 1376
BstNI CCWGG 2 cut(s) 739, 1384
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 737, 1382
BstSFI CTRYAG 2 cut(s) 588, 1368
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 2 cut(s) 1282, 1354
BstX2I RGATCY 2 cut(s) 628, 1066
BstYI RGATCY 2 cut(s) 628, 1066
BstZ17I GTATAC 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 1551
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 3 cut(s) 64, 1110, 1350
BsuTUI ATCGAT 1 cut(s) 1551
BtgI CCRYGG 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 2 cut(s) 14, 176
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 4 cut(s) 549, 788, 1231, 1446
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 3 cut(s) 558, 1112, 1131
CciI TCATGA 1 cut(s) 931
CfoI GCGC 2 cut(s) 95, 130
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 4 cut(s) 52, 63, 1334, 1386
ClaI ATCGAT 1 cut(s) 1551
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 398
CviAII CATG 5 cut(s) 131, 521, 557, 932, 1033
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 3 cut(s) 755, 1266, 1330
DpnI GATC 9 cut(s) 216, 426, 630, 648, 966, 1068, 1101, 1550, 1554
DpnII GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
DraI TTTAAA 2 cut(s) 297, 729
EaeI YGGCCR 2 cut(s) 1108, 1348
Eco24I GRGCYC 1 cut(s) 689
Eco47I GGWCC 3 cut(s) 52, 1334, 1386
Eco57I CTGAAG 2 cut(s) 297, 729
Eco88I CYCGRG 3 cut(s) 158, 266, 1079
EcoO109I RGGNCCY 2 cut(s) 52, 1386
EcoRI GAATTC 1 cut(s) 1117
EcoRII CCWGG 2 cut(s) 737, 1382
EcoT38I GRGCYC 1 cut(s) 689
FaeI CATG 5 cut(s) 134, 524, 560, 935, 1036
FalI AAGNNNNNCTT 4 cut(s) 420, 452, 634, 666
FaqI GGGAC 2 cut(s) 65, 1372
FatI CATG 5 cut(s) 130, 520, 556, 931, 1032
FauI CCCGC 3 cut(s) 41, 150, 919
FblI GTMKAC 2 cut(s) 231, 369
Fnu4HI GCNGC 4 cut(s) 31, 1296, 1368, 1380
FokI GGATG 2 cut(s) 21, 163
FriOI GRGCYC 1 cut(s) 689
Fsp4HI GCNGC 4 cut(s) 31, 1296, 1368, 1380
FspBI CTAG 4 cut(s) 257, 579, 1011, 1071
GlaI GCGC 2 cut(s) 94, 129
GluI GCNGC 4 cut(s) 31, 1296, 1368, 1380
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 64, 1110, 1350
HapII CCGG 3 cut(s) 302, 1111, 1560
HgaI GACGC 1 cut(s) 47
HhaI GCGC 2 cut(s) 95, 130
Hin1I GRCGYC 3 cut(s) 21, 39, 198
Hin1II CATG 5 cut(s) 134, 524, 560, 935, 1036
Hin6I GCGC 2 cut(s) 93, 128
HinP1I GCGC 2 cut(s) 93, 128
HincII GTYRAC 3 cut(s) 498, 1000, 1360
HindII GTYRAC 3 cut(s) 498, 1000, 1360
HinfI GANTC 5 cut(s) 409, 499, 1082, 1262, 1440
HpaI GTTAAC 1 cut(s) 1360
HpaII CCGG 3 cut(s) 302, 1111, 1560
HphI GGTGA 3 cut(s) 235, 910, 1489
Hpy166II GTNNAC 6 cut(s) 232, 370, 498, 901, 1000, 1360
Hpy188I TCNGA 7 cut(s) 19, 414, 463, 504, 773, 1267, 1277
Hpy188III TCNNGA 8 cut(s) 194, 238, 268, 340, 428, 932, 1332, 1457
Hpy8I GTNNAC 6 cut(s) 232, 370, 498, 901, 1000, 1360
Hpy99I CGWCG 2 cut(s) 92, 1342
HpyAV CCTTC 6 cut(s) 437, 940, 973, 1200, 1277, 1463
HpyCH4III ACNGT 4 cut(s) 553, 673, 768, 1225
HpyCH4IV ACGT 5 cut(s) 21, 198, 494, 1340, 1416
HpyCH4V TGCA 9 cut(s) 349, 556, 702, 763, 1040, 1090, 1172, 1256, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 151, 1301, 1376
HpyF3I CTNAG 3 cut(s) 755, 1266, 1330
HpySE526I ACGT 5 cut(s) 21, 198, 494, 1340, 1416
Hsp92I GRCGYC 3 cut(s) 21, 39, 198
Hsp92II CATG 5 cut(s) 134, 524, 560, 935, 1036
HspAI GCGC 2 cut(s) 93, 128
KroI GCCGGC 1 cut(s) 1110
KroNI GCCGGC 1 cut(s) 1112
KspAI GTTAAC 1 cut(s) 1360
Kzo9I GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
Lsp1109I GCAGC 2 cut(s) 1282, 1354
LweI GCATC 5 cut(s) 94, 185, 1159, 1459, 1493
MaeI CTAG 4 cut(s) 257, 579, 1011, 1071
MaeII ACGT 5 cut(s) 21, 198, 494, 1340, 1416
MaeIII GTNAC 2 cut(s) 199, 469
MalI GATC 9 cut(s) 216, 426, 630, 648, 966, 1068, 1101, 1550, 1554
MboI GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
MboII GAAGA 3 cut(s) 253, 909, 1484
MflI RGATCY 2 cut(s) 628, 1066
MhlI GDGCHC 2 cut(s) 613, 689
MlsI TGGCCA 1 cut(s) 1350
MluNI TGGCCA 1 cut(s) 1350
MlyI GAGTC 3 cut(s) 403, 493, 1091
MmeI TCCRAC 3 cut(s) 1255, 1422, 1495
Mox20I TGGCCA 1 cut(s) 1350
MroNI GCCGGC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 1350
MseI TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 1350
MspA1I CMGCKG 1 cut(s) 606
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 3 cut(s) 302, 1111, 1560
MspR9I CCNGG 2 cut(s) 739, 1384
MvaI CCWGG 2 cut(s) 739, 1384
MwoI GCNNNNNNNGC 4 cut(s) 134, 151, 1301, 1376
NaeI GCCGGC 1 cut(s) 1112
NdeII GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
NgoMIV GCCGGC 1 cut(s) 1110
NlaIII CATG 5 cut(s) 134, 524, 560, 935, 1036
NlaIV GGNNCC 2 cut(s) 53, 1388
NmeAIII GCCGAG 2 cut(s) 84, 586
NmuCI GTSAC 2 cut(s) 199, 469
NspI RCATGY 1 cut(s) 560
PaeI GCATGC 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 1112
PfeI GAWTC 2 cut(s) 1262, 1440
PkrI GCNGC 4 cut(s) 32, 1297, 1369, 1381
PleI GAGTC 3 cut(s) 403, 493, 1090
PpsI GAGTC 3 cut(s) 403, 493, 1090
PpuMI RGGWCCY 2 cut(s) 52, 1386
PsiI TTATAA 1 cut(s) 1430
Psp1406I AACGTT 1 cut(s) 494
Psp5II RGGWCCY 2 cut(s) 52, 1386
Psp6I CCWGG 2 cut(s) 737, 1382
PspGI CCWGG 2 cut(s) 737, 1382
PspN4I GGNNCC 2 cut(s) 53, 1388
PspPI GGNCC 4 cut(s) 52, 63, 1334, 1386
PspPPI RGGWCCY 2 cut(s) 52, 1386
PspXI VCTCGAGB 1 cut(s) 158
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 2 cut(s) 628, 1066
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
SatI GCNGC 4 cut(s) 31, 1296, 1368, 1380
Sau3AI GATC 9 cut(s) 214, 424, 628, 646, 964, 1066, 1099, 1548, 1552
Sau96I GGNCC 4 cut(s) 52, 63, 1334, 1386
SchI GAGTC 3 cut(s) 403, 493, 1091
ScrFI CCNGG 2 cut(s) 739, 1384
SduI GDGCHC 2 cut(s) 613, 689
SfaNI GCATC 5 cut(s) 94, 185, 1159, 1459, 1493
SfcI CTRYAG 2 cut(s) 588, 1368
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 3 cut(s) 52, 1334, 1386
SlaI CTCGAG 1 cut(s) 158
SmiI ATTTAAAT 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 158, 236, 655
SmoI CTYRAG 3 cut(s) 158, 236, 655
SphI GCATGC 1 cut(s) 560
SsiI CCGC 7 cut(s) 30, 48, 143, 604, 926, 1380, 1529
SspMI CTAG 4 cut(s) 257, 579, 1011, 1071
StyD4I CCNGG 2 cut(s) 737, 1382
SwaI ATTTAAAT 1 cut(s) 297
TaaI ACNGT 4 cut(s) 553, 673, 768, 1225
TaiI ACGT 5 cut(s) 24, 201, 497, 1343, 1419
TaqI TCGA 4 cut(s) 159, 339, 484, 1551
TaqII GACCGA 1 cut(s) 1351
TauI GCSGC 2 cut(s) 33, 1382
TfiI GAWTC 2 cut(s) 1262, 1440
Tru1I TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
Tru9I TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
TscAI CASTG 4 cut(s) 556, 795, 1238, 1453
TseFI GTSAC 2 cut(s) 199, 469
TseI GCWGC 2 cut(s) 1295, 1367
Tsp45I GTSAC 2 cut(s) 199, 469
TspDTI ATGAA 2 cut(s) 948, 1021
TspGWI ACGGA 2 cut(s) 353, 1092
TspRI CASTG 4 cut(s) 556, 795, 1238, 1453
Vha464I CTTAAG 1 cut(s) 655
VpaK11BI GGWCC 3 cut(s) 52, 1334, 1386
XapI RAATTY 4 cut(s) 352, 415, 599, 1117
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 231, 369
XspI CTAG 4 cut(s) 257, 579, 1011, 1071
ZraI GACGTC 2 cut(s) 22, 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.