Rh3BG052100

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000862
Physical Location & Seq
Forward (+)
3558074 .. 3563581
5508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG052100.1

Sequence Viewer

Length: 1752 bp
ATGGGAGGGTGTTTCTCCGACGTGAAAGGTGGCAAGCAGGCAGTGGGAGGGGCCCATCAACAACAGAGGCCGATCAACAAGGACGCTGACTCAAACGACGCCGTTGAGCTCTTTAACCGCTCTCGTGGGGTTTATCCTCTCTACACTCCGCTCGAGTTATCGCTGTCAGCAACTAACTTGCTTGATCGTGACATTATGTCAAAGAGTGATCCTATGGTTGTGGTTTATGTGAAGAAAGGAGATGGTAAGCTAGAGGAACTAGGAAGAACTGAAGTAATACTGAATAGTTTGAAACCAGTGTGGATTGAGAAAATCACAGTCTCATATCAGTTCGAGATTGTGCAGTCATTGATATTTCATGTCTATGATGTCGATACAAAATATCACAACGTAAATGTAAAGACACTGAAGTTGGAGGATCAAGAATGTCTTGGGGAAGGCAGTTGCGTTCTTTCAGAGATAGTGACCAAAAAAAGTCGAAGTTTAACCTTAAATCTGCATAACAGAAGTGGGCGTGGCTTAGGAACACTCACAGTCCATGCTGAAGAAACTATGGCTTCGAAGAGTGTTGTTGAGATAAAATTCTGTTGTTCCCAATTAGAAAACAAAGACCTCTTCTCTAAAAGTGATCCCTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTAGTGGATAACAATTTAAATCCAATTTGGAAACCACTATCCCTCAGTATGCAGCAGTTTGGAAGCAAGGATAATCCATTAGTCATCGAGTGCTTTGATTTCAACAGCAATGGCAATCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCAGACCTGGAGAGATTATACAAAGAAAGAAGTGGGGTGAATTTCGTCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTTGTGGATCAATTTAATGAGAAGCAACAGTTCAGCTTTCTCGATTATATTTCCAGTGGATTTGAACTTAACTTCATGGTTGCGGTTGACTTTACGGCTTCAAATGGAAATCCTCAACAACATGATTCGTTGCACTACATTGATCCGTATGGCCGATTAAATTCTTACCAGCAGGCAGTAACAGAAGTTGGGGAAGTCATTCAGTTTTATGATACTGACAAACGCTTTCCTGCTTGGGGATTTGGAGGAAGGACTGCTGGAGGACCTGTATCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTTGAAGGGGTTCAAGGCATAATGACTGCGTATGCCAGTGCCCTACACAATGTTTCTCTATCAGGACCCACTTTGTTTGGCCCAGTGGTTACTAGAGCTGCAGAGATTGCTGGCAATTCTCTATTCCACAACAACAATAAGTACTTTGTGCTGCTGATCATAACGGATGGGATACTTACAGACCTGCAGGAAACAACAGATGCTTTGGTGAGGGCATCTGATCTTCCCCTCTCAGTTCTTATAGTTGGAGTGGGAAGTGCGGATTTCAAACAAATGGAGATTCTCGATGCTGACAATGGACAGCGATTATCAAGTTCAACAGGCCGTGTGGCTACCAGAGACATTGTACAATTTGTTCCTATGAGAGAAGTGCAGAGTGGGCGCATTTCTGCTGTTCAATCGCTTCTTGAAGAGCTACCTGGACAGTTCTTGAGTTACATGCGCTGCAGAGACGTCATGCCTCTCACTGTCCATGCAGCCCAATCATCTGCTCCACAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

583

Amino Acids

64.23

Weight (kDa)

5.6

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 55 - 152 5.4e-12 C2 domain
C2 PF00168 196 - 285 2.2e-12 C2 domain
Copine PF07002 355 - 568 2.7e-76 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1704
Acc36I ACCTGC 1 cut(s) 1440
AccBSI CCGCTC 2 cut(s) 120, 151
AciI CCGC 4 cut(s) 118, 149, 1019, 1508
AclWI GGATC 5 cut(s) 203, 426, 623, 951, 1073
AcoI YGGCCR 1 cut(s) 1087
AcsI RAATTY 3 cut(s) 581, 883, 1096
AcuI CTGAAG 4 cut(s) 291, 428, 564, 711
AcyI GRCGYC 2 cut(s) 99, 1701
AfaI GTAC 2 cut(s) 1391, 1596
AfiI CCNNNNNNNGG 1 cut(s) 1172
AflII CTTAAG 1 cut(s) 637
AhdI GACNNNNNGTC 1 cut(s) 196
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 849, 1666
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
AluBI AGCT 5 cut(s) 109, 250, 972, 1346, 1663
AluI AGCT 5 cut(s) 109, 250, 972, 1346, 1663
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 3 cut(s) 325, 1581, 1692
AlwI GGATC 5 cut(s) 203, 426, 623, 951, 1073
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 5 cut(s) 51, 68, 1087, 1327, 1570
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 5 cut(s) 745, 1346, 1399, 1692, 1724
ApoI RAATTY 3 cut(s) 581, 883, 1096
Asp700I GAANNNNTTC 2 cut(s) 1134, 1257
AspLEI GCGC 2 cut(s) 1632, 1692
AspS9I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
AsuHPI GGTGA 2 cut(s) 892, 1468
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 1199, 1313
BaeGI GKGCMC 2 cut(s) 55, 1291
BanII GRGCYC 2 cut(s) 55, 111
BauI CACGAG 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 757, 1333, 1386, 1679, 1736
BccI CCATC 5 cut(s) 63, 236, 904, 1220, 1409
BceAI ACGGC 3 cut(s) 86, 1047, 1557
BciT130I CCWGG 2 cut(s) 851, 1668
BciVI GTATCC 1 cut(s) 1413
BclI TGATCA 1 cut(s) 1404
BcoDI GTCTC 3 cut(s) 325, 1581, 1692
BfaI CTAG 4 cut(s) 251, 260, 648, 1341
BfmI CTRYAG 3 cut(s) 1347, 1433, 1693
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 1440
BfuI GTATCC 1 cut(s) 1413
BisI GCNGC 5 cut(s) 746, 1347, 1400, 1693, 1725
BlsI GCNGC 5 cut(s) 747, 1348, 1401, 1694, 1726
BmcAI AGTACT 1 cut(s) 1391
Bme1390I CCNGG 2 cut(s) 851, 1668
Bme18I GGWCC 2 cut(s) 1199, 1313
BmeRI GACNNNNNGTC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 152
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
BmiI GGNNCC 3 cut(s) 52, 53, 1315
BmrFI CCNGG 2 cut(s) 851, 1668
BmrI ACTGGG 1 cut(s) 1325
BmsI GCATC 3 cut(s) 1438, 1472, 1525
BmuI ACTGGG 1 cut(s) 1325
BpmI CTGGAG 2 cut(s) 872, 1215
Bpu10I CCTNAGC 1 cut(s) 520
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 1699
BsaHI GRCGYC 2 cut(s) 99, 1701
BsaXI ACNNNNNCTCC 4 cut(s) 657, 687, 1188, 1218
Bsc4I CCNNNNNNNGG 1 cut(s) 1172
Bse1I ACTGG 5 cut(s) 296, 990, 1243, 1284, 1331
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 2 cut(s) 851, 1668
BseGI GGATG 2 cut(s) 1231, 1420
BseLI CCNNNNNNNGG 1 cut(s) 1172
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 2 cut(s) 751, 1494
BseNI ACTGG 5 cut(s) 296, 990, 1243, 1284, 1331
BseSI GKGCMC 2 cut(s) 55, 1291
BseXI GCAGC 5 cut(s) 757, 1333, 1386, 1679, 1736
BsgI GTGCAG 2 cut(s) 362, 1640
BshFI GGCC 5 cut(s) 53, 70, 1089, 1329, 1572
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 1172
BsmAI GTCTC 3 cut(s) 325, 1581, 1692
BsmBI CGTCTC 1 cut(s) 1692
BsnI GGCC 5 cut(s) 53, 70, 1089, 1329, 1572
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 3 cut(s) 55, 111, 1291
Bsp1407I TGTACA 1 cut(s) 1594
Bsp143I GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
BspACI CCGC 4 cut(s) 118, 149, 1019, 1508
BspANI GGCC 5 cut(s) 53, 70, 1089, 1329, 1572
BspCNI CTCAG 2 cut(s) 750, 1493
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 3 cut(s) 52, 53, 1315
BspMAI CTGCAG 3 cut(s) 1351, 1437, 1697
BspMI ACCTGC 1 cut(s) 1440
BspPI GGATC 5 cut(s) 203, 426, 623, 951, 1073
BspQI GCTCTTC 1 cut(s) 1653
BspT104I TTCGAA 1 cut(s) 560
BspTI CTTAAG 1 cut(s) 637
BsrBI CCGCTC 2 cut(s) 120, 151
BsrDI GCAATG 1 cut(s) 808
BsrGI TGTACA 1 cut(s) 1594
BsrI ACTGG 5 cut(s) 296, 990, 1243, 1284, 1331
BssMI GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
BssNI GRCGYC 2 cut(s) 99, 1701
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 851, 1668
Bst4CI ACNGT 6 cut(s) 319, 535, 966, 1214, 1674, 1717
Bst6I CTCTTC 3 cut(s) 557, 620, 1653
BstACI GRCGYC 2 cut(s) 99, 1701
BstAFI CTTAAG 1 cut(s) 637
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 1594
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 35, 39, 1110, 1360
BstDEI CTNAG 3 cut(s) 520, 737, 1480
BstF5I GGATG 2 cut(s) 1231, 1420
BstHHI GCGC 2 cut(s) 1632, 1692
BstKTI GATC 9 cut(s) 75, 187, 211, 421, 631, 946, 1081, 1407, 1471
BstMAI GTCTC 3 cut(s) 325, 1581, 1692
BstMBI GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
BstMWI GCNNNNNNNGC 2 cut(s) 1355, 1627
BstNI CCWGG 2 cut(s) 851, 1668
BstNSI RCATGY 1 cut(s) 1690
BstSCI CCNGG 2 cut(s) 849, 1666
BstSFI CTRYAG 3 cut(s) 1347, 1433, 1693
BstSLI GKGCMC 2 cut(s) 55, 1291
BstV1I GCAGC 5 cut(s) 757, 1333, 1386, 1679, 1736
BsuI GTATCC 1 cut(s) 1413
BsuRI GGCC 5 cut(s) 53, 70, 1089, 1329, 1572
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 1231, 1420
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 9 cut(s) 48, 303, 404, 846, 997, 1210, 1291, 1338, 1713
BveI ACCTGC 1 cut(s) 1440
Cac8I GCNNGC 4 cut(s) 35, 39, 1110, 1360
CciI TCATGA 1 cut(s) 910
CfoI GCGC 2 cut(s) 1632, 1692
Cfr13I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
CseI GACGC 2 cut(s) 92, 107
Csp6I GTAC 2 cut(s) 1390, 1595
CspCI CAANNNNNGTGG 2 cut(s) 1306, 1341
CviQI GTAC 2 cut(s) 1390, 1595
DdeI CTNAG 3 cut(s) 520, 737, 1480
DpnI GATC 9 cut(s) 74, 186, 210, 420, 630, 945, 1080, 1406, 1470
DpnII GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
DraI TTTAAA 1 cut(s) 711
DriI GACNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 1087
Eam1104I CTCTTC 3 cut(s) 557, 620, 1653
Eam1105I GACNNNNNGTC 1 cut(s) 196
EarI CTCTTC 3 cut(s) 557, 620, 1653
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 2 cut(s) 55, 111
Eco47I GGWCC 2 cut(s) 1199, 1313
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 4 cut(s) 291, 428, 564, 711
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 3 cut(s) 51, 1199, 1313
EcoRII CCWGG 2 cut(s) 849, 1666
EcoT38I GRGCYC 2 cut(s) 55, 111
Esp3I CGTCTC 1 cut(s) 1692
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FbaI TGATCA 1 cut(s) 1404
Fnu4HI GCNGC 5 cut(s) 746, 1347, 1400, 1693, 1725
FokI GGATG 2 cut(s) 1238, 1427
FriOI GRGCYC 2 cut(s) 55, 111
Fsp4HI GCNGC 5 cut(s) 746, 1347, 1400, 1693, 1725
FspBI CTAG 4 cut(s) 251, 260, 648, 1341
GlaI GCGC 2 cut(s) 1631, 1691
GluI GCNGC 5 cut(s) 746, 1347, 1400, 1693, 1725
GsuI CTGGAG 2 cut(s) 872, 1215
HaeIII GGCC 5 cut(s) 53, 70, 1089, 1329, 1572
HgaI GACGC 2 cut(s) 92, 107
HhaI GCGC 2 cut(s) 1632, 1692
Hin1I GRCGYC 2 cut(s) 99, 1701
Hin6I GCGC 2 cut(s) 1630, 1690
HinP1I GCGC 2 cut(s) 1630, 1690
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HinfI GANTC 3 cut(s) 89, 1061, 1528
HphI GGTGA 2 cut(s) 892, 1468
Hpy166II GTNNAC 1 cut(s) 1024
Hpy188I TCNGA 3 cut(s) 19, 457, 1468
Hpy8I GTNNAC 1 cut(s) 1024
Hpy99I CGWCG 2 cut(s) 23, 101
HpyAV CCTTC 4 cut(s) 431, 643, 1179, 1247
HpyCH4III ACNGT 6 cut(s) 319, 535, 966, 1214, 1674, 1717
HpyCH4IV ACGT 3 cut(s) 21, 390, 1701
HpyF10VI GCNNNNNNNGC 2 cut(s) 1355, 1627
HpyF3I CTNAG 3 cut(s) 520, 737, 1480
HpySE526I ACGT 3 cut(s) 21, 390, 1701
Hsp92I GRCGYC 2 cut(s) 99, 1701
HspAI GCGC 2 cut(s) 1630, 1690
Ksp22I TGATCA 1 cut(s) 1404
Kzo9I GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
LguI GCTCTTC 1 cut(s) 1653
LmnI GCTCC 1 cut(s) 1744
Lsp1109I GCAGC 5 cut(s) 757, 1333, 1386, 1679, 1736
LweI GCATC 3 cut(s) 1438, 1472, 1525
MaeI CTAG 4 cut(s) 251, 260, 648, 1341
MaeII ACGT 3 cut(s) 21, 390, 1701
MaeIII GTNAC 5 cut(s) 188, 463, 1114, 1336, 1682
MalI GATC 9 cut(s) 74, 186, 210, 420, 630, 945, 1080, 1406, 1470
MbiI CCGCTC 2 cut(s) 120, 151
MboI GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
MboII GAAGA 8 cut(s) 244, 276, 557, 574, 607, 891, 1463, 1670
MhlI GDGCHC 3 cut(s) 55, 111, 1291
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 3 cut(s) 42, 393, 1474
MroXI GAANNNNTTC 2 cut(s) 1134, 1257
MseI TTAA 9 cut(s) 114, 485, 491, 638, 710, 951, 1005, 1094, 1218
MslI CAYNNNNRTG 2 cut(s) 363, 909
MspCI CTTAAG 1 cut(s) 637
MspR9I CCNGG 2 cut(s) 851, 1668
MvaI CCWGG 2 cut(s) 851, 1668
MwoI GCNNNNNNNGC 2 cut(s) 1355, 1627
NdeII GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
NlaIV GGNNCC 3 cut(s) 52, 53, 1315
NmuCI GTSAC 2 cut(s) 188, 463
NspI RCATGY 1 cut(s) 1690
NspV TTCGAA 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 910
PciSI GCTCTTC 1 cut(s) 1653
PdmI GAANNNNTTC 2 cut(s) 1134, 1257
PfeI GAWTC 2 cut(s) 1061, 1528
PkrI GCNGC 5 cut(s) 747, 1348, 1401, 1694, 1726
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PpuMI RGGWCCY 2 cut(s) 1199, 1313
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 2 cut(s) 1199, 1313
Psp6I CCWGG 2 cut(s) 849, 1666
PspGI CCWGG 2 cut(s) 849, 1666
PspN4I GGNNCC 3 cut(s) 52, 53, 1315
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
PspPPI RGGWCCY 2 cut(s) 1199, 1313
PspXI VCTCGAGB 1 cut(s) 152
PsrI GAACNNNNNNTAC 2 cut(s) 1587, 1619
PstI CTGCAG 3 cut(s) 1351, 1437, 1697
RsaI GTAC 2 cut(s) 1391, 1596
RsaNI GTAC 2 cut(s) 1390, 1595
RseI CAYNNNNRTG 2 cut(s) 363, 909
SacI GAGCTC 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 1653
SaqAI TTAA 9 cut(s) 114, 485, 491, 638, 710, 951, 1005, 1094, 1218
SatI GCNGC 5 cut(s) 746, 1347, 1400, 1693, 1725
Sau3AI GATC 9 cut(s) 72, 184, 208, 418, 628, 943, 1078, 1404, 1468
Sau96I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
SbfI CCTGCAGG 1 cut(s) 1437
ScaI AGTACT 1 cut(s) 1391
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 851, 1668
SdaI CCTGCAGG 1 cut(s) 1437
SduI GDGCHC 3 cut(s) 55, 111, 1291
SfaNI GCATC 3 cut(s) 1438, 1472, 1525
SfcI CTRYAG 3 cut(s) 1347, 1433, 1693
Sfr274I CTCGAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 560
SinI GGWCC 2 cut(s) 1199, 1313
SlaI CTCGAG 1 cut(s) 152
SmiI ATTTAAAT 1 cut(s) 711
SmiMI CAYNNNNRTG 2 cut(s) 363, 909
SmlI CTYRAG 3 cut(s) 152, 637, 1678
SmoI CTYRAG 3 cut(s) 152, 637, 1678
Sse8387I CCTGCAGG 1 cut(s) 1437
SsiI CCGC 4 cut(s) 118, 149, 1019, 1508
SspMI CTAG 4 cut(s) 251, 260, 648, 1341
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 849, 1666
SwaI ATTTAAAT 1 cut(s) 711
TaaI ACNGT 6 cut(s) 319, 535, 966, 1214, 1674, 1717
TaiI ACGT 3 cut(s) 24, 393, 1704
TaqI TCGA 8 cut(s) 153, 333, 372, 478, 560, 780, 978, 1533
TatI WGTACW 2 cut(s) 1389, 1594
TfiI GAWTC 2 cut(s) 1061, 1528
Tru1I TTAA 9 cut(s) 114, 485, 491, 638, 710, 951, 1005, 1094, 1218
Tru9I TTAA 9 cut(s) 114, 485, 491, 638, 710, 951, 1005, 1094, 1218
TscAI CASTG 9 cut(s) 48, 303, 411, 846, 997, 1217, 1291, 1338, 1720
TseFI GTSAC 2 cut(s) 188, 463
TseI GCWGC 5 cut(s) 745, 1346, 1399, 1692, 1724
Tsp45I GTSAC 2 cut(s) 188, 463
TspDTI ATGAA 3 cut(s) 347, 927, 1000
TspGWI ACGGA 2 cut(s) 1071, 1427
TspRI CASTG 9 cut(s) 48, 303, 411, 846, 997, 1217, 1291, 1338, 1720
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 1199, 1313
XapI RAATTY 3 cut(s) 581, 883, 1096
XbaI TCTAGA 1 cut(s) 647
XceI RCATGY 1 cut(s) 1690
XhoI CTCGAG 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 1134, 1257
XspI CTAG 4 cut(s) 251, 260, 648, 1341
ZraI GACGTC 1 cut(s) 1702
ZrmI AGTACT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.