pycom12g21280

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
22483545 .. 22488113
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21280.7

Sequence Viewer

Length: 1593 bp
ATGGGAGGATGCTTTTCGGACGTGAGGGGAGGCAAGGACGCCGTGGGCGGGGCCCTGCCACGGCCCACCGAACCGAACGCCAAGAACGACGGCGCTCACAACGATGCCATTGAATTCTTTTACAGGGAGCATGGTGCTTACCCGCTTTTTACGCAACTCGAGTTATCCTTTTCAGCATCCAAGCTGCTTGATCTTGATGTGACTTCAAAGAGTGATCCTATGGTGGTTGTATACCTCAAGAAGAATGATGGAAAGCTAGAGGAACTCGGGAGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGATGGTGCATAATTTGATATTCCGTGTCTACGATGTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCGATCTAGATCGGAAGAGTTTCTTGGAGAAGGCAGTTGTGTCCTTTCAGAGATTGTGACTAAACAAAATCGAAGTCTAACGTTGACTCTGAAAGGCAAAAACGGACATGGAGGTTTGAGAAACTTTGGAACACTCACTGTGCATGCCGAGGAAACTATTGCTTCTAGGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTCTTTTCTAAAAGCGATCCTTTCTTAAGAATATCAAGGACTGTTGAGACTGGGGGTTCTATTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACACCCCACTGGTTATTGAGTGTTTTGATTTCAACAGCGATGGCAACCACGTTCTTATTGGGAAACTCCAAAAATCAGTAGCAGACCTTGAAAAACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGATCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAAGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACAGGTTCAAATGGAGATCCTAGAAAACCCGAGTCTTTGCACTATATCGATCCTTATGGCCGGCTGAATTCTTACCAACACGCTATAACAGAAGTAGGACAAGTCATTCAGTTCTACGATGCTGATAAGAGTTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCACACTGCTTCAACTTGAATGGAAATGCAAGTGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCTACGCAATGTTGCTCTGTCAGGACCGACGTTGTTTGGCCAAGTGGTTAACAGGGCTGCTGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAACAAGTACTACGTCTTGCTCATTATAACGGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAATGCGGATTTCAGACAAATGGAGCAAAACTTGACGCTGACAAAGGGCAACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

531

Amino Acids

58.61

Weight (kDa)

5.72

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1442
AccI GTMKAC 2 cut(s) 231, 369
AciI CCGC 6 cut(s) 48, 143, 616, 938, 1392, 1541
AclI AACGTT 1 cut(s) 506
AclWI GGATC 7 cut(s) 209, 432, 653, 948, 984, 1073, 1106
AcoI YGGCCR 2 cut(s) 1120, 1360
AcsI RAATTY 4 cut(s) 113, 415, 611, 1129
AcuI CTGAAG 2 cut(s) 297, 741
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 399, 1424
AfiI CCNNNNNNNGG 3 cut(s) 48, 60, 1205
AflII CTTAAG 1 cut(s) 667
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 749, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 6 cut(s) 184, 256, 329, 967, 1005, 1036
AluI AGCT 6 cut(s) 184, 256, 329, 967, 1005, 1036
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 7 cut(s) 209, 432, 653, 948, 984, 1073, 1106
Ama87I CYCGRG 3 cut(s) 158, 266, 1091
AoxI GGCC 4 cut(s) 51, 62, 1120, 1360
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 184, 1307, 1379
ApoI RAATTY 4 cut(s) 113, 415, 611, 1129
ArsI GACNNNNNNTTYG 4 cut(s) 400, 432, 484, 516
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 5 cut(s) 51, 52, 63, 1346, 1398
AsuHPI GGTGA 2 cut(s) 922, 1501
AvaI CYCGRG 3 cut(s) 158, 266, 1091
AvaII GGWCC 2 cut(s) 1346, 1398
BaeGI GKGCMC 2 cut(s) 55, 625
BalI TGGCCA 1 cut(s) 1362
BanII GRGCYC 1 cut(s) 55
BauI CACGAG 1 cut(s) 933
BbvI GCAGC 3 cut(s) 171, 1294, 1366
BccI CCATC 5 cut(s) 242, 337, 827, 934, 1442
BceAI ACGGC 4 cut(s) 26, 77, 106, 1239
BciT130I CCWGG 2 cut(s) 751, 1396
BciVI GTATCC 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 5 cut(s) 257, 432, 591, 1023, 1083
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 667
BfuI GTATCC 1 cut(s) 303
BglII AGATCT 1 cut(s) 640
BisI GCNGC 4 cut(s) 185, 1308, 1380, 1392
BlsI GCNGC 4 cut(s) 186, 1309, 1381, 1393
BmcAI AGTACT 1 cut(s) 1424
Bme1390I CCNGG 2 cut(s) 751, 1396
Bme18I GGWCC 2 cut(s) 1346, 1398
BmeT110I CYCGRG 3 cut(s) 158, 266, 1091
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 5 cut(s) 51, 52, 63, 1346, 1398
BmiI GGNNCC 3 cut(s) 52, 53, 1400
BmrFI CCNGG 2 cut(s) 751, 1396
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 94, 185, 1171, 1471, 1505
BmuI ACTGGG 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 221
Bsa29I ATCGAT 2 cut(s) 427, 1110
BsaBI GATNNNNATC 1 cut(s) 433
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 42, 59, 573
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 60, 1205
Bse118I RCCGGY 1 cut(s) 1122
Bse1I ACTGG 2 cut(s) 697, 807
Bse3DI GCAATG 1 cut(s) 1336
Bse8I GATNNNNATC 1 cut(s) 433
BseBI CCWGG 2 cut(s) 751, 1396
BseCI ATCGAT 2 cut(s) 427, 1110
BseDI CCNNGG 3 cut(s) 42, 59, 573
BseGI GGATG 3 cut(s) 14, 176, 1453
BseJI GATNNNNATC 1 cut(s) 433
BseLI CCNNNNNNNGG 3 cut(s) 48, 60, 1205
BseMI GCAATG 1 cut(s) 1336
BseMII CTCAG 2 cut(s) 758, 1269
BseNI ACTGG 2 cut(s) 697, 807
BseSI GKGCMC 2 cut(s) 55, 625
BseXI GCAGC 3 cut(s) 171, 1294, 1366
BshFI GGCC 4 cut(s) 53, 64, 1122, 1362
BshVI ATCGAT 2 cut(s) 427, 1110
BsiHKCI CYCGRG 3 cut(s) 158, 266, 1091
BsiSI CCGG 2 cut(s) 302, 1123
BslFI GGGAC 1 cut(s) 1384
BslI CCNNNNNNNGG 3 cut(s) 48, 60, 1205
BsmAI GTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 1384
BsnI GGCC 4 cut(s) 53, 64, 1122, 1362
BsoBI CYCGRG 3 cut(s) 158, 266, 1091
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 55, 625
BspACI CCGC 6 cut(s) 48, 143, 616, 938, 1392, 1541
BspANI GGCC 4 cut(s) 53, 64, 1122, 1362
BspCNI CTCAG 2 cut(s) 759, 1270
BspDI ATCGAT 2 cut(s) 427, 1110
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 3 cut(s) 52, 53, 1400
BspPI GGATC 7 cut(s) 209, 432, 653, 948, 984, 1073, 1106
BspTI CTTAAG 1 cut(s) 667
BsrDI GCAATG 1 cut(s) 1336
BsrFI RCCGGY 1 cut(s) 1122
BsrI ACTGG 2 cut(s) 697, 807
BssAI RCCGGY 1 cut(s) 1122
BssECI CCNNGG 3 cut(s) 42, 59, 573
BssNAI GTATAC 1 cut(s) 232
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 933
Bst1107I GTATAC 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 933
Bst2UI CCWGG 2 cut(s) 751, 1396
Bst4CI ACNGT 4 cut(s) 565, 685, 780, 1237
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 570, 1124
BstDEI CTNAG 2 cut(s) 767, 1278
BstDSI CCRYGG 2 cut(s) 42, 59
BstF5I GGATG 3 cut(s) 14, 176, 1453
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstMAI GTCTC 1 cut(s) 683
BstMWI GCNNNNNNNGC 3 cut(s) 151, 1313, 1388
BstNI CCWGG 2 cut(s) 751, 1396
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 749, 1394
BstSFI CTRYAG 1 cut(s) 600
BstSLI GKGCMC 2 cut(s) 55, 625
BstV1I GCAGC 3 cut(s) 171, 1294, 1366
BstX2I RGATCY 2 cut(s) 640, 1078
BstYI RGATCY 2 cut(s) 640, 1078
BstZ17I GTATAC 1 cut(s) 232
Bsu15I ATCGAT 2 cut(s) 427, 1110
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 4 cut(s) 53, 64, 1122, 1362
BsuTUI ATCGAT 2 cut(s) 427, 1110
BtgI CCRYGG 2 cut(s) 42, 59
BtgZI GCGATG 1 cut(s) 846
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 14, 176, 1453
BtsI GCAGTG 1 cut(s) 1243
BtsIMutI CAGTG 4 cut(s) 561, 800, 1243, 1458
Cac8I GCNNGC 2 cut(s) 570, 1124
CciI TCATGA 1 cut(s) 943
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 1122
Cfr13I GGNCC 5 cut(s) 51, 52, 63, 1346, 1398
ClaI ATCGAT 2 cut(s) 427, 1110
CseI GACGC 2 cut(s) 47, 1579
Csp6I GTAC 2 cut(s) 398, 1423
CviAII CATG 5 cut(s) 131, 533, 569, 944, 1045
CviQI GTAC 2 cut(s) 398, 1423
DdeI CTNAG 2 cut(s) 767, 1278
DraI TTTAAA 2 cut(s) 297, 741
EaeI YGGCCR 2 cut(s) 1120, 1360
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 1346, 1398
Eco57I CTGAAG 2 cut(s) 297, 741
Eco88I CYCGRG 3 cut(s) 158, 266, 1091
EcoO109I RGGNCCY 3 cut(s) 51, 52, 1398
EcoRI GAATTC 2 cut(s) 113, 1129
EcoRII CCWGG 2 cut(s) 749, 1394
EcoT38I GRGCYC 1 cut(s) 55
FaeI CATG 5 cut(s) 134, 536, 572, 947, 1048
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 646, 678
FaqI GGGAC 1 cut(s) 1384
FatI CATG 5 cut(s) 130, 532, 568, 943, 1044
FauI CCCGC 2 cut(s) 41, 150
FblI GTMKAC 2 cut(s) 231, 369
Fnu4HI GCNGC 4 cut(s) 185, 1308, 1380, 1392
FokI GGATG 3 cut(s) 21, 163, 1460
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 4 cut(s) 185, 1308, 1380, 1392
FspBI CTAG 5 cut(s) 257, 432, 591, 1023, 1083
GlaI GCGC 1 cut(s) 94
GluI GCNGC 4 cut(s) 185, 1308, 1380, 1392
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 53, 64, 1122, 1362
HapII CCGG 2 cut(s) 302, 1123
HgaI GACGC 2 cut(s) 47, 1579
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 5 cut(s) 134, 536, 572, 947, 1048
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 3 cut(s) 510, 1012, 1372
HindII GTYRAC 3 cut(s) 510, 1012, 1372
HinfI GANTC 5 cut(s) 409, 511, 1094, 1274, 1452
HpaI GTTAAC 1 cut(s) 1372
HpaII CCGG 2 cut(s) 302, 1123
HphI GGTGA 2 cut(s) 922, 1501
Hpy166II GTNNAC 6 cut(s) 232, 370, 510, 913, 1012, 1372
Hpy188I TCNGA 9 cut(s) 19, 414, 439, 475, 516, 785, 1279, 1289, 1550
Hpy188III TCNNGA 8 cut(s) 194, 238, 268, 340, 432, 944, 1344, 1469
Hpy8I GTNNAC 6 cut(s) 232, 370, 510, 913, 1012, 1372
Hpy99I CGWCG 2 cut(s) 92, 1354
HpyAV CCTTC 5 cut(s) 449, 952, 1212, 1289, 1475
HpyCH4III ACNGT 4 cut(s) 565, 685, 780, 1237
HpyCH4IV ACGT 5 cut(s) 21, 506, 843, 1352, 1428
HpyCH4V TGCA 7 cut(s) 349, 568, 714, 775, 1052, 1102, 1268
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 1313, 1388
HpyF3I CTNAG 2 cut(s) 767, 1278
HpySE526I ACGT 5 cut(s) 21, 506, 843, 1352, 1428
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 5 cut(s) 134, 536, 572, 947, 1048
HspAI GCGC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 1122
KroNI GCCGGC 1 cut(s) 1124
KspAI GTTAAC 1 cut(s) 1372
LmnI GCTCC 2 cut(s) 127, 1558
Lsp1109I GCAGC 3 cut(s) 171, 1294, 1366
LweI GCATC 5 cut(s) 94, 185, 1171, 1471, 1505
MaeI CTAG 5 cut(s) 257, 432, 591, 1023, 1083
MaeII ACGT 5 cut(s) 21, 506, 843, 1352, 1428
MaeIII GTNAC 2 cut(s) 199, 481
MboII GAAGA 4 cut(s) 253, 452, 921, 1496
MflI RGATCY 2 cut(s) 640, 1078
MhlI GDGCHC 2 cut(s) 55, 625
MlsI TGGCCA 1 cut(s) 1362
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 3 cut(s) 403, 505, 1103
MmeI TCCRAC 3 cut(s) 1267, 1434, 1507
Mox20I TGGCCA 1 cut(s) 1362
MroNI GCCGGC 1 cut(s) 1122
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 6 cut(s) 296, 609, 668, 740, 1038, 1371
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 1362
MspA1I CMGCKG 1 cut(s) 618
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 2 cut(s) 302, 1123
MspR9I CCNGG 2 cut(s) 751, 1396
MvaI CCWGG 2 cut(s) 751, 1396
MwoI GCNNNNNNNGC 3 cut(s) 151, 1313, 1388
NaeI GCCGGC 1 cut(s) 1124
NgoMIV GCCGGC 1 cut(s) 1122
NlaIII CATG 5 cut(s) 134, 536, 572, 947, 1048
NlaIV GGNNCC 3 cut(s) 52, 53, 1400
NmeAIII GCCGAG 1 cut(s) 598
NmuCI GTSAC 2 cut(s) 199, 481
NspI RCATGY 1 cut(s) 572
PaeI GCATGC 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 1124
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 1274, 1452
PkrI GCNGC 4 cut(s) 186, 1309, 1381, 1393
PleI GAGTC 3 cut(s) 403, 505, 1102
PpsI GAGTC 3 cut(s) 403, 505, 1102
PpuMI RGGWCCY 1 cut(s) 1398
PsiI TTATAA 1 cut(s) 1442
Psp1406I AACGTT 1 cut(s) 506
Psp5II RGGWCCY 1 cut(s) 1398
Psp6I CCWGG 2 cut(s) 749, 1394
PspGI CCWGG 2 cut(s) 749, 1394
PspN4I GGNNCC 3 cut(s) 52, 53, 1400
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 5 cut(s) 51, 52, 63, 1346, 1398
PspPPI RGGWCCY 1 cut(s) 1398
PspXI VCTCGAGB 1 cut(s) 158
PsuI RGATCY 2 cut(s) 640, 1078
RsaI GTAC 2 cut(s) 399, 1424
RsaNI GTAC 2 cut(s) 398, 1423
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 6 cut(s) 296, 609, 668, 740, 1038, 1371
SatI GCNGC 4 cut(s) 185, 1308, 1380, 1392
Sau96I GGNCC 5 cut(s) 51, 52, 63, 1346, 1398
ScaI AGTACT 1 cut(s) 1424
SchI GAGTC 3 cut(s) 403, 505, 1103
ScrFI CCNGG 2 cut(s) 751, 1396
SduI GDGCHC 2 cut(s) 55, 625
SfaNI GCATC 5 cut(s) 94, 185, 1171, 1471, 1505
SfcI CTRYAG 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 2 cut(s) 1346, 1398
SlaI CTCGAG 1 cut(s) 158
SmiI ATTTAAAT 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 158, 236, 667
SmoI CTYRAG 3 cut(s) 158, 236, 667
SphI GCATGC 1 cut(s) 572
SsiI CCGC 6 cut(s) 48, 143, 616, 938, 1392, 1541
SspMI CTAG 5 cut(s) 257, 432, 591, 1023, 1083
StyD4I CCNGG 2 cut(s) 749, 1394
SwaI ATTTAAAT 1 cut(s) 297
TaaI ACNGT 4 cut(s) 565, 685, 780, 1237
TaiI ACGT 5 cut(s) 24, 509, 846, 1355, 1431
TaqI TCGA 5 cut(s) 159, 339, 427, 496, 1110
TaqII GACCGA 1 cut(s) 1363
TatI WGTACW 1 cut(s) 1422
TauI GCSGC 1 cut(s) 1394
TfiI GAWTC 2 cut(s) 1274, 1452
Tru1I TTAA 6 cut(s) 296, 609, 668, 740, 1038, 1371
Tru9I TTAA 6 cut(s) 296, 609, 668, 740, 1038, 1371
TscAI CASTG 4 cut(s) 568, 807, 1250, 1465
TseFI GTSAC 2 cut(s) 199, 481
TseI GCWGC 3 cut(s) 184, 1307, 1379
Tsp45I GTSAC 2 cut(s) 199, 481
TspDTI ATGAA 2 cut(s) 960, 1033
TspGWI ACGGA 3 cut(s) 353, 543, 1460
TspRI CASTG 4 cut(s) 568, 807, 1250, 1465
Vha464I CTTAAG 1 cut(s) 667
VpaK11BI GGWCC 2 cut(s) 1346, 1398
XapI RAATTY 4 cut(s) 113, 415, 611, 1129
XbaI TCTAGA 1 cut(s) 431
XceI RCATGY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 231, 369
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 5 cut(s) 257, 432, 591, 1023, 1083
ZrmI AGTACT 1 cut(s) 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.