Rroxscaffold_6G00430350

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000862
Physical Location & Seq
Forward (+)
50512967 .. 50516711
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430350.1

Sequence Viewer

Length: 1635 bp
ATGTTGGATTTCTTATCTGTGTTACAGTTATCGCTGTCAGCAACTAACTTGCTTGATCGTGACATTACGTCAAAGAGTGATCCCATGGTTGTGGTTTATGTGAAGAAAGGAGATGGCAAGCTAGAGGAACTAGGGAGAACTGAAGTAATACTGAATAGTTTGAACCCAGTGTGGATTGAGAAAATTACAGTTTCATATCAGTTCGAGGTTGTGCAGCCATTGATATTCCACGTCTATGATGTCGATACAAAATATCACAATGTGAATGTAAAGACACTGAAGTTGGAGGATCAAGAATTTCTTGGTGAAGGCAGTTGTGTTCTTTCAGAGATAGTGACCAAACAAAGTCGAAGTTTAACCTTTAATCTGCATAACAGAAGTGGGCGTGGAGGTATGAGAAACTTCGGAACACTCACTGTCCATGCTGAAGAAACTATGGCTTCAAAGAGTGTAGTTGAAATAAAATTCCGTTGTTCCCAATTGGAAAACAAAGACCTCTTCTCTAAAAGTGATCCTTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAATCCAATCTGGAAACCACTATCCCTGAGTATGCAGCAGTTTGGAAGCAAGGATAATCCATTAGTCATCGAGTGCTTTGATTTCAACAGCAATGGCAACCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCAGACCTTGAGAGATTATACCAAGAAAGAAGTGGGGTGAATTTTGCCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTTGTGGATCAATTTAATGAGAAGCAACAGTTCAGCTTTCTTGATTATATTTCCAGTGGATTTGAGCTTAACTTCATGGTTGCTGTTGACTTTACGGCTTCAAATGGAAACCCTCAACAACATGATTCGTTGCACTACATTGATCCGTATGGCCGGTTGAATTCCTACCAGCAGGCAATAACGGAGGTTGGGGAAGTCATTCAGTTTTATGATGCTGATAAACGGTTTCCTGCTTGGGGCTTTGGAGGAAGGACAGCTGGAGGGCCTGTATCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTCGAAGGAGTTCAAGGGATAATGACTGCTTATGCCAGTGCCCTACACAATGTTTCTCTCTCAGGACCCACTTTGTTTGGCCCAGTGGTTACTAGGGCTGCAGAGATTGCTGGCAATTCTCTATTCCACAACAACAATAAGTACTATGTGTTGCTGATCATAACGGATGGGATACTTACAGACCTGCAGGAAACAACAGATGCTTTGGTGCGGGCATCTGATCTTCCCCTCTCAGTTCTTATTGTTGGAGTAGGAAGTGCGGATTTCAAACAAATGGAGATTCTTGATGCTGACAATGGACCGCGATTGTCAAGTTCAACAGGTCGTGTGGCTACCAGAGACATTGTACAGTTTGTTCCTATGAGAGAAGTGCAGAGTGGGCGCATTTCTGCGGTTCAATCGCTTCTCGAAGAGCTGCCTGGACAGTTCTTGGGTTACATGCGCTGCAGAGACATCATGCCTCTCACTGTCCATGCAACCCAATCATCTGCTCCACAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

544

Amino Acids

60.2

Weight (kDa)

5.38

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 121 2.9e-14 C2 domain
C2 PF00168 157 - 246 1.1e-12 C2 domain
Copine PF07002 316 - 530 9.1e-77 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1323
AccII CGCG 1 cut(s) 1435
AciI CCGC 4 cut(s) 1342, 1391, 1433, 1523
AclWI GGATC 5 cut(s) 74, 297, 506, 834, 956
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 4 cut(s) 296, 464, 766, 979
AcuI CTGAAG 4 cut(s) 162, 299, 447, 594
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 2 cut(s) 1274, 1479
AfiI CCNNNNNNNGG 1 cut(s) 1055
AflII CTTAAG 1 cut(s) 520
AhdI GACNNNNNGTC 1 cut(s) 67
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 1549
AloI GAACNNNNNNTCC 2 cut(s) 540, 572
AluBI AGCT 5 cut(s) 121, 855, 886, 1076, 1546
AluI AGCT 5 cut(s) 121, 855, 886, 1076, 1546
Alw26I GTCTC 2 cut(s) 1464, 1575
AlwI GGATC 5 cut(s) 74, 297, 506, 834, 956
AoxI GGCC 3 cut(s) 970, 1082, 1210
ApeKI GCWGC 5 cut(s) 214, 628, 1229, 1546, 1575
ApoI RAATTY 4 cut(s) 296, 464, 766, 979
Asp700I GAANNNNTTC 2 cut(s) 1017, 1140
AspLEI GCGC 2 cut(s) 1515, 1575
AspS9I GGNCC 4 cut(s) 1082, 1196, 1211, 1430
AsuHPI GGTGA 2 cut(s) 317, 775
AvaII GGWCC 2 cut(s) 1196, 1430
BaeGI GKGCMC 1 cut(s) 1174
BbvI GCAGC 5 cut(s) 226, 640, 1216, 1533, 1562
BccI CCATC 4 cut(s) 107, 787, 1103, 1292
BceAI ACGGC 1 cut(s) 930
BciT130I CCWGG 1 cut(s) 1551
BciVI GTATCC 1 cut(s) 1296
BclI TGATCA 1 cut(s) 1287
BcoDI GTCTC 2 cut(s) 1464, 1575
BfaI CTAG 4 cut(s) 122, 131, 531, 1224
BfmI CTRYAG 3 cut(s) 1230, 1316, 1576
BfrI CTTAAG 1 cut(s) 520
BfuAI ACCTGC 1 cut(s) 1323
BfuI GTATCC 1 cut(s) 1296
BisI GCNGC 5 cut(s) 215, 629, 1230, 1547, 1576
BlsI GCNGC 5 cut(s) 216, 630, 1231, 1548, 1577
BmcAI AGTACT 1 cut(s) 1274
Bme1390I CCNGG 1 cut(s) 1551
Bme18I GGWCC 2 cut(s) 1196, 1430
BmeRI GACNNNNNGTC 1 cut(s) 67
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 4 cut(s) 1082, 1196, 1211, 1430
BmiI GGNNCC 1 cut(s) 1198
BmrFI CCNGG 1 cut(s) 1551
BmrI ACTGGG 2 cut(s) 161, 1208
BmsI GCATC 4 cut(s) 1021, 1321, 1355, 1408
BmuI ACTGGG 2 cut(s) 161, 1208
BpmI CTGGAG 1 cut(s) 1098
BpuEI CTTGAG 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 84
BsaXI ACNNNNNCTCC 4 cut(s) 540, 570, 1071, 1101
Bsc4I CCNNNNNNNGG 1 cut(s) 1055
Bse118I RCCGGY 1 cut(s) 972
Bse1I ACTGG 5 cut(s) 167, 873, 1126, 1167, 1214
Bse3DI GCAATG 1 cut(s) 691
BseBI CCWGG 1 cut(s) 1551
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 2 cut(s) 1114, 1303
BseLI CCNNNNNNNGG 1 cut(s) 1055
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 3 cut(s) 611, 1206, 1377
BseNI ACTGG 5 cut(s) 167, 873, 1126, 1167, 1214
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 5 cut(s) 226, 640, 1216, 1533, 1562
BsgI GTGCAG 2 cut(s) 233, 1523
Bsh1236I CGCG 1 cut(s) 1435
BshFI GGCC 3 cut(s) 972, 1084, 1212
BsiSI CCGG 1 cut(s) 973
BslI CCNNNNNNNGG 1 cut(s) 1055
BsmAI GTCTC 2 cut(s) 1464, 1575
BsnI GGCC 3 cut(s) 972, 1084, 1212
Bsp1286I GDGCHC 1 cut(s) 1174
Bsp1407I TGTACA 1 cut(s) 1477
Bsp143I GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 4 cut(s) 1342, 1391, 1433, 1523
BspANI GGCC 3 cut(s) 972, 1084, 1212
BspCNI CTCAG 3 cut(s) 612, 1205, 1376
BspFNI CGCG 1 cut(s) 1435
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 1 cut(s) 1198
BspMAI CTGCAG 3 cut(s) 1234, 1320, 1580
BspMI ACCTGC 1 cut(s) 1323
BspPI GGATC 5 cut(s) 74, 297, 506, 834, 956
BspQI GCTCTTC 1 cut(s) 1536
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 691
BsrFI RCCGGY 1 cut(s) 972
BsrGI TGTACA 1 cut(s) 1477
BsrI ACTGG 5 cut(s) 167, 873, 1126, 1167, 1214
BssAI RCCGGY 1 cut(s) 972
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 1551
Bst4CI ACNGT 9 cut(s) 27, 190, 418, 849, 1044, 1097, 1482, 1557, 1600
Bst6I CTCTTC 2 cut(s) 503, 1536
BstAFI CTTAAG 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstAUI TGTACA 1 cut(s) 1477
BstC8I GCNNGC 4 cut(s) 119, 993, 1243, 1344
BstDEI CTNAG 3 cut(s) 620, 1192, 1363
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 1114, 1303
BstFNI CGCG 1 cut(s) 1435
BstHHI GCGC 2 cut(s) 1515, 1575
BstKTI GATC 8 cut(s) 58, 82, 292, 514, 829, 964, 1290, 1354
BstMAI GTCTC 2 cut(s) 1464, 1575
BstMBI GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
BstMWI GCNNNNNNNGC 3 cut(s) 1090, 1238, 1510
BstNI CCWGG 1 cut(s) 1551
BstNSI RCATGY 1 cut(s) 1573
BstSCI CCNGG 1 cut(s) 1549
BstSFI CTRYAG 3 cut(s) 1230, 1316, 1576
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 1 cut(s) 1435
BstV1I GCAGC 5 cut(s) 226, 640, 1216, 1533, 1562
BstXI CCANNNNNNTGG 1 cut(s) 91
BsuI GTATCC 1 cut(s) 1296
BsuRI GGCC 3 cut(s) 972, 1084, 1212
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 1114, 1303
BtsIMutI CAGTG 9 cut(s) 174, 275, 414, 729, 880, 1093, 1174, 1221, 1596
BveI ACCTGC 1 cut(s) 1323
Cac8I GCNNGC 4 cut(s) 119, 993, 1243, 1344
CciI TCATGA 1 cut(s) 793
CfoI GCGC 2 cut(s) 1515, 1575
Cfr10I RCCGGY 1 cut(s) 972
Cfr13I GGNCC 4 cut(s) 1082, 1196, 1211, 1430
Csp6I GTAC 2 cut(s) 1273, 1478
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CviQI GTAC 2 cut(s) 1273, 1478
DdeI CTNAG 3 cut(s) 620, 1192, 1363
DpnI GATC 8 cut(s) 57, 81, 291, 513, 828, 963, 1289, 1353
DpnII GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
DraI TTTAAA 1 cut(s) 594
DraIII CACNNNGTG 1 cut(s) 262
DriI GACNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 2 cut(s) 503, 1536
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 2 cut(s) 503, 1536
Eco130I CCWWGG 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 1196, 1430
Eco57I CTGAAG 4 cut(s) 162, 299, 447, 594
EcoO109I RGGNCCY 2 cut(s) 1082, 1196
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 1 cut(s) 1549
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FalI AAGNNNNNCTT 4 cut(s) 285, 317, 499, 531
FauI CCCGC 1 cut(s) 1335
FbaI TGATCA 1 cut(s) 1287
Fnu4HI GCNGC 5 cut(s) 215, 629, 1230, 1547, 1576
FokI GGATG 2 cut(s) 1121, 1310
Fsp4HI GCNGC 5 cut(s) 215, 629, 1230, 1547, 1576
FspBI CTAG 4 cut(s) 122, 131, 531, 1224
GlaI GCGC 2 cut(s) 1514, 1574
GluI GCNGC 5 cut(s) 215, 629, 1230, 1547, 1576
GsuI CTGGAG 1 cut(s) 1098
HaeIII GGCC 3 cut(s) 972, 1084, 1212
HapII CCGG 1 cut(s) 973
HhaI GCGC 2 cut(s) 1515, 1575
Hin6I GCGC 2 cut(s) 1513, 1573
HinP1I GCGC 2 cut(s) 1513, 1573
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HinfI GANTC 2 cut(s) 944, 1411
HpaII CCGG 1 cut(s) 973
HphI GGTGA 2 cut(s) 317, 775
Hpy166II GTNNAC 1 cut(s) 907
Hpy188I TCNGA 3 cut(s) 328, 407, 1351
Hpy188III TCNNGA 9 cut(s) 59, 293, 531, 604, 794, 860, 1194, 1415, 1538
Hpy8I GTNNAC 1 cut(s) 907
HpyAV CCTTC 3 cut(s) 302, 1062, 1130
HpyCH4III ACNGT 9 cut(s) 27, 190, 418, 849, 1044, 1097, 1482, 1557, 1600
HpyCH4IV ACGT 2 cut(s) 68, 231
HpyF10VI GCNNNNNNNGC 3 cut(s) 1090, 1238, 1510
HpyF3I CTNAG 3 cut(s) 620, 1192, 1363
HpySE526I ACGT 2 cut(s) 68, 231
HspAI GCGC 2 cut(s) 1513, 1573
Ksp22I TGATCA 1 cut(s) 1287
Kzo9I GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
LguI GCTCTTC 1 cut(s) 1536
LmnI GCTCC 1 cut(s) 1627
Lsp1109I GCAGC 5 cut(s) 226, 640, 1216, 1533, 1562
LweI GCATC 4 cut(s) 1021, 1321, 1355, 1408
MaeI CTAG 4 cut(s) 122, 131, 531, 1224
MaeII ACGT 2 cut(s) 68, 231
MaeIII GTNAC 5 cut(s) 21, 59, 334, 1219, 1565
MalI GATC 8 cut(s) 57, 81, 291, 513, 828, 963, 1289, 1353
MboI GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
MboII GAAGA 6 cut(s) 115, 440, 490, 774, 1346, 1553
MfeI CAATTG 1 cut(s) 479
MhlI GDGCHC 1 cut(s) 1174
MmeI TCCRAC 2 cut(s) 264, 1357
MroXI GAANNNNTTC 2 cut(s) 1017, 1140
MseI TTAA 7 cut(s) 356, 363, 521, 593, 834, 888, 1101
MslI CAYNNNNRTG 3 cut(s) 89, 234, 792
MspA1I CMGCKG 1 cut(s) 1076
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 1 cut(s) 973
MspR9I CCNGG 1 cut(s) 1551
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 1551
MvnI CGCG 1 cut(s) 1435
MwoI GCNNNNNNNGC 3 cut(s) 1090, 1238, 1510
NcoI CCATGG 1 cut(s) 84
NdeII GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
NlaIV GGNNCC 1 cut(s) 1198
NmuCI GTSAC 2 cut(s) 59, 334
NspI RCATGY 1 cut(s) 1573
PagI TCATGA 1 cut(s) 793
PciSI GCTCTTC 1 cut(s) 1536
PdmI GAANNNNTTC 2 cut(s) 1017, 1140
PfeI GAWTC 2 cut(s) 944, 1411
PkrI GCNGC 5 cut(s) 216, 630, 1231, 1548, 1577
PpuMI RGGWCCY 1 cut(s) 1196
Psp5II RGGWCCY 1 cut(s) 1196
Psp6I CCWGG 1 cut(s) 1549
PspGI CCWGG 1 cut(s) 1549
PspN4I GGNNCC 1 cut(s) 1198
PspPI GGNCC 4 cut(s) 1082, 1196, 1211, 1430
PspPPI RGGWCCY 1 cut(s) 1196
PsrI GAACNNNNNNTAC 2 cut(s) 1470, 1502
PstI CTGCAG 3 cut(s) 1234, 1320, 1580
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 2 cut(s) 1274, 1479
RsaNI GTAC 2 cut(s) 1273, 1478
RseI CAYNNNNRTG 3 cut(s) 89, 234, 792
SapI GCTCTTC 1 cut(s) 1536
SaqAI TTAA 7 cut(s) 356, 363, 521, 593, 834, 888, 1101
SatI GCNGC 5 cut(s) 215, 629, 1230, 1547, 1576
Sau3AI GATC 8 cut(s) 55, 79, 289, 511, 826, 961, 1287, 1351
Sau96I GGNCC 4 cut(s) 1082, 1196, 1211, 1430
SbfI CCTGCAGG 1 cut(s) 1320
ScaI AGTACT 1 cut(s) 1274
ScrFI CCNGG 1 cut(s) 1551
SdaI CCTGCAGG 1 cut(s) 1320
SduI GDGCHC 1 cut(s) 1174
SfaNI GCATC 4 cut(s) 1021, 1321, 1355, 1408
SfcI CTRYAG 3 cut(s) 1230, 1316, 1576
SinI GGWCC 2 cut(s) 1196, 1430
SmiI ATTTAAAT 1 cut(s) 594
SmiMI CAYNNNNRTG 3 cut(s) 89, 234, 792
SmlI CTYRAG 2 cut(s) 520, 734
SmoI CTYRAG 2 cut(s) 520, 734
Sse8387I CCTGCAGG 1 cut(s) 1320
SsiI CCGC 4 cut(s) 1342, 1391, 1433, 1523
SspMI CTAG 4 cut(s) 122, 131, 531, 1224
StyD4I CCNGG 1 cut(s) 1549
StyI CCWWGG 1 cut(s) 84
SwaI ATTTAAAT 1 cut(s) 594
TaaI ACNGT 9 cut(s) 27, 190, 418, 849, 1044, 1097, 1482, 1557, 1600
TaiI ACGT 2 cut(s) 71, 234
TaqI TCGA 6 cut(s) 204, 243, 349, 663, 1134, 1539
TatI WGTACW 2 cut(s) 1272, 1477
TfiI GAWTC 2 cut(s) 944, 1411
Tru1I TTAA 7 cut(s) 356, 363, 521, 593, 834, 888, 1101
Tru9I TTAA 7 cut(s) 356, 363, 521, 593, 834, 888, 1101
TscAI CASTG 9 cut(s) 174, 282, 421, 729, 880, 1100, 1174, 1221, 1603
TseFI GTSAC 2 cut(s) 59, 334
TseI GCWGC 5 cut(s) 214, 628, 1229, 1546, 1575
Tsp45I GTSAC 2 cut(s) 59, 334
TspDTI ATGAA 3 cut(s) 183, 810, 883
TspGWI ACGGA 4 cut(s) 458, 954, 1016, 1310
TspRI CASTG 9 cut(s) 174, 282, 421, 729, 880, 1100, 1174, 1221, 1603
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 1196, 1430
XapI RAATTY 4 cut(s) 296, 464, 766, 979
XbaI TCTAGA 1 cut(s) 530
XceI RCATGY 1 cut(s) 1573
XcmI CCANNNNNNNNNTGG 2 cut(s) 701, 755
XmnI GAANNNNTTC 2 cut(s) 1017, 1140
XspI CTAG 4 cut(s) 122, 131, 531, 1224
ZrmI AGTACT 1 cut(s) 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.