Rorug02G0284800

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000862
Physical Location & Seq
Forward (+)
31004496 .. 31008051
3556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0284800.1

Sequence Viewer

Length: 819 bp
ATGTTTTCCAGTCCTTCAATTCCAGTGATTCAAAATTTAGTTGGATTTGAGGAGGAGTTGTTGGAGAAGGAGGTGACGAAGGCGGCGGAGTCGCTATCGTCTCGCACGCTTAACCCCACGAACCCAACACACCCATTTCTCCAGAACGCACTCAACTCCTCCGTCGTAGATTCCAAGACCACAAAACAAGGAAATACAGTCCTGATTCCTGAACCATGTCCTTCCAGCTTCACAATTGACAAGAACAAGTGCGTGCCACTTTCAATGGAGAACCAGAAGAATAATAAGAGACTCTCTGCTAATGTTCCTGTTCAACTTCATGAGAGGTTTCAAGGTGAGAGTATCACTAGGTCCTCAGTTATAGAGAAACCTGGAGACCATGGAGAAGCACACTTATTGAATGATCTGATAGCGGCAGGGGACATTAAGCATTGGAAATATTTGCCAATGGTAACGTTTGAGAGCAAAATTTTGGAATATCTAGGTCGAAAATGCATCAGCCATGAAGACCGTCGATTGAGTTTTGATTGGGATTCTGGAAGCACACACGTCTATCATTGTTACGTCAACATGGATGGGAGTTACAGATTCAAGCCAGCCAGAACTGTTGATTTTGGCACCTGTGATCAATCGGTTCCAGAGGCATATCCAGATAAGGATGAGGAGACAAATGGCTACTTGGCACAATATTCATCAACTGGTTTTACTGATATGGGGAACACAACTGATGAAGGTTCTTATTTTGGCAAGGACAAATCATTGGAGCTCAATCTATTCAAGGTTGATAGAACATACCTAATGAGAAAATTAGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

272

Amino Acids

30.64

Weight (kDa)

5.65

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 617
AciI CCGC 3 cut(s) 83, 86, 413
AclI AACGTT 1 cut(s) 455
AcsI RAATTY 2 cut(s) 34, 468
AflIII ACRYGT 1 cut(s) 547
AgsI TTSAA 8 cut(s) 18, 32, 264, 314, 332, 400, 592, 778
AjiI CACGTC 1 cut(s) 550
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 228, 766
AluI AGCT 2 cut(s) 228, 766
Alw21I GWGCWC 1 cut(s) 768
Alw26I GTCTC 4 cut(s) 105, 283, 369, 659
ApoI RAATTY 2 cut(s) 34, 468
AspS9I GGNCC 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 85, 347
AvaII GGWCC 1 cut(s) 351
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 768
BccI CCATC 1 cut(s) 569
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 4 cut(s) 105, 283, 369, 659
BfaI CTAG 2 cut(s) 348, 482
BisI GCNGC 2 cut(s) 84, 414
BlsI GCNGC 2 cut(s) 85, 415
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 1 cut(s) 351
BmgBI CACGTC 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 2 cut(s) 619, 636
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 2 cut(s) 125, 393
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bse1I ACTGG 3 cut(s) 9, 23, 703
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 2 cut(s) 580, 664
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 3 cut(s) 9, 23, 703
BseRI GAGGAG 4 cut(s) 65, 68, 148, 677
BshNI GGYRCC 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 768
BslFI GGGAC 1 cut(s) 434
BsmAI GTCTC 4 cut(s) 105, 283, 369, 659
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 434
Bso31I GGTCTC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 768
Bsp143I GATC 2 cut(s) 403, 625
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 3 cut(s) 83, 86, 413
BspCNI CTCAG 1 cut(s) 368
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 619, 636
BspT107I GGYRCC 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 369
BsrI ACTGG 3 cut(s) 9, 23, 703
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 2 cut(s) 403, 625
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 199, 512, 607
BstC8I GCNNGC 3 cut(s) 107, 254, 597
BstDEI CTNAG 1 cut(s) 355
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 2 cut(s) 580, 664
BstKTI GATC 2 cut(s) 406, 628
BstMAI GTCTC 4 cut(s) 105, 283, 369, 659
BstMBI GATC 2 cut(s) 403, 625
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 379
BtrI CACGTC 1 cut(s) 550
BtsCI GGATG 2 cut(s) 580, 664
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 3 cut(s) 107, 254, 597
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 351
CviAII CATG 5 cut(s) 216, 320, 380, 503, 571
CviJI RGCY 6 cut(s) 228, 501, 595, 599, 675, 766
CviKI_1 RGCY 6 cut(s) 228, 501, 595, 599, 675, 766
DdeI CTNAG 1 cut(s) 355
DpnI GATC 2 cut(s) 405, 627
DpnII GATC 2 cut(s) 403, 625
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 768
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 766
EcoICRI GAGCTC 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 497
EcoT38I GRGCYC 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 5 cut(s) 219, 323, 383, 506, 574
FaiI YATR 9 cut(s) 217, 321, 362, 381, 504, 572, 646, 713, 793
FaqI GGGAC 1 cut(s) 434
FatI CATG 5 cut(s) 215, 319, 379, 502, 570
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 84, 414
FokI GGATG 2 cut(s) 587, 671
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 2 cut(s) 84, 414
FspBI CTAG 2 cut(s) 348, 482
GluI GCNGC 2 cut(s) 84, 414
GsuI CTGGAG 2 cut(s) 125, 393
Hin1II CATG 5 cut(s) 219, 323, 383, 506, 574
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 7 cut(s) 28, 89, 170, 205, 291, 533, 588
HphI GGTGA 2 cut(s) 85, 347
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 7 cut(s) 142, 202, 209, 320, 537, 638, 650
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 2 cut(s) 167, 516
HpyAV CCTTC 5 cut(s) 24, 61, 73, 231, 725
HpyCH4III ACNGT 3 cut(s) 199, 512, 607
HpyCH4IV ACGT 3 cut(s) 455, 549, 564
HpyCH4V TGCA 1 cut(s) 495
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 3 cut(s) 455, 549, 564
Hsp92II CATG 5 cut(s) 219, 323, 383, 506, 574
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 2 cut(s) 403, 625
LmnI GCTCC 1 cut(s) 763
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 348, 482
MaeII ACGT 3 cut(s) 455, 549, 564
MaeIII GTNAC 4 cut(s) 73, 451, 560, 581
MalI GATC 2 cut(s) 405, 627
MboI GATC 2 cut(s) 403, 625
MboII GAAGA 2 cut(s) 289, 518
MfeI CAATTG 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 768
MluCI AATT 5 cut(s) 18, 34, 234, 468, 806
MlyI GAGTC 2 cut(s) 98, 285
MmeI TCCRAC 2 cut(s) 22, 42
MnlI CCTC 8 cut(s) 43, 46, 64, 169, 318, 364, 634, 655
Mph1103I ATGCAT 1 cut(s) 497
MseI TTAA 2 cut(s) 111, 426
MspR9I CCNGG 1 cut(s) 372
MunI CAATTG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 372
NcoI CCATGG 1 cut(s) 379
NdeII GATC 2 cut(s) 403, 625
NlaIII CATG 5 cut(s) 219, 323, 383, 506, 574
NlaIV GGNNCC 2 cut(s) 619, 636
NmuCI GTSAC 1 cut(s) 73
NsiI ATGCAT 1 cut(s) 497
PagI TCATGA 1 cut(s) 319
PfeI GAWTC 5 cut(s) 28, 170, 205, 533, 588
PkrI GCNGC 2 cut(s) 85, 415
PleI GAGTC 2 cut(s) 97, 285
PpsI GAGTC 2 cut(s) 97, 285
PpuMI RGGWCCY 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 768
Psp1406I AACGTT 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 619, 636
PspPI GGNCC 1 cut(s) 351
PspPPI RGGWCCY 1 cut(s) 351
SacI GAGCTC 1 cut(s) 768
SaqAI TTAA 2 cut(s) 111, 426
SatI GCNGC 2 cut(s) 84, 414
Sau3AI GATC 2 cut(s) 403, 625
Sau96I GGNCC 1 cut(s) 351
SchI GAGTC 2 cut(s) 98, 285
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 768
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 1 cut(s) 351
Sse9I AATT 5 cut(s) 18, 34, 234, 468, 806
SsiI CCGC 3 cut(s) 83, 86, 413
SspI AATATT 2 cut(s) 440, 689
SspMI CTAG 2 cut(s) 348, 482
SstI GAGCTC 1 cut(s) 768
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 3 cut(s) 199, 512, 607
TaiI ACGT 3 cut(s) 458, 552, 567
TaqI TCGA 2 cut(s) 487, 514
TasI AATT 5 cut(s) 18, 34, 234, 468, 806
TauI GCSGC 2 cut(s) 86, 416
TfiI GAWTC 5 cut(s) 28, 170, 205, 533, 588
Tru1I TTAA 2 cut(s) 111, 426
Tru9I TTAA 2 cut(s) 111, 426
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 4 cut(s) 308, 519, 681, 744
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 30
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 2 cut(s) 34, 468
XspI CTAG 2 cut(s) 348, 482
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.