MD12G1227900.v1.1

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
30337289 .. 30341496
4208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1227900.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGTGTTCCAAATGTTTGACCAAAATATTATCCTTTTCAGCATCCAAGCTGCTTGATCTTGATGTCACTTCAAAGAGTGATCCTATGGTGGTTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTCGGGAGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGATGGTGCAGAATTTGATACGTTTGCTTATTTTCTTTTCTTGTGTTCTCCTCCAAGATTCCGTGTCTACGATGTTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCAAGAGTTTCTTGGAGAAGGCAGTTGTGTTCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTTAACATTGACTCTGAACGGCAAAAACGGGCTTGGAGGTTTGAGAAACTTTGGAACACTCACCGTGCATGCCGAGGAAACTATTGCTTCTACGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTCTTTTCTAAAAGCGATCCTTTCTTAAGAATATCAAGAACTGTTGAGACTGGGGGTTCTATTCCAATTTGCAAGACTGAGGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACACCCCACTGGTTTTTGAGTGTTTTGATTTCAACAGCAATGGCAACCACATTCTTATTGGGAAACTCCAAAAATCAGTAGCAGACCTTGAAAAACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAAGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACGGGTTCAAATGGAGATCCTAGAAAGCCCGAGTCTTTGCACTATATTGATCCTTATGGCCGGCTGAATTCTTACCAGCAGGTTATAACAGAAGTAGGACAAGTCATTCAGTTCTATGATGCTGATAAGAGTTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCACACTGCTTCAACTTGAATGGAAATGCAAGTGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCTACGCAATGTTGCTCTGTCAGGACCGACGTTGTTTGGCCAAGTGGTTAACAGGGCTGCTGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAACAAGTACTACGTCTTGCTCATTATAACAGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAACGCGGATTTCAAACAAATGGAGATCCTTGACGCTGACAACGGGCAACGATTAGAAAGTTCTACAGGTCGCATAGCTACGCGTGACATTGTCCAGTTCGTTCCAATGAGAGATGTGCAGAGTGGGCAGATTTCTGTTGTTCAAGGTCTTCTGGAAGAGCTGCCCGGACAGTTTTTGAGTTACATGCGTTCCATGGACATCAAGCCGCTCACCTTCAATGCCGCTGCCCAAGCATCATCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

558

Amino Acids

61.46

Weight (kDa)

5.57

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 74 2.1e-07 C2 domain
C2 PF00168 170 - 259 5e-12 C2 domain
Copine PF07002 330 - 545 2.4e-80 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1040, 1334
Acc36I ACCTGC 1 cut(s) 1024
AccBSI CCGCTC 1 cut(s) 1636
AccI GTMKAC 2 cut(s) 96, 272
AccII CGCG 2 cut(s) 1433, 1510
AciI CCGC 6 cut(s) 508, 830, 1284, 1433, 1634, 1650
AclWI GGATC 7 cut(s) 74, 336, 545, 876, 965, 998, 1447
AcoI YGGCCR 2 cut(s) 1012, 1252
AcsI RAATTY 4 cut(s) 217, 319, 503, 1021
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 303, 1316
AfiI CCNNNNNNNGG 1 cut(s) 1097
AflII CTTAAG 1 cut(s) 559
AflIII ACRYGT 1 cut(s) 1508
AjnI CCWGG 2 cut(s) 641, 1286
AluBI AGCT 8 cut(s) 49, 121, 194, 859, 897, 928, 1505, 1588
AluI AGCT 8 cut(s) 49, 121, 194, 859, 897, 928, 1505, 1588
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 7 cut(s) 74, 336, 545, 876, 965, 998, 1447
Ama87I CYCGRG 2 cut(s) 131, 983
AoxI GGCC 2 cut(s) 1012, 1252
ApeKI GCWGC 5 cut(s) 49, 1199, 1271, 1588, 1652
ApoI RAATTY 4 cut(s) 217, 319, 503, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
AspS9I GGNCC 2 cut(s) 1238, 1290
AsuC2I CCSGG 1 cut(s) 1593
AsuHPI GGTGA 4 cut(s) 445, 814, 1393, 1630
AvaI CYCGRG 2 cut(s) 131, 983
AvaII GGWCC 2 cut(s) 1238, 1290
BaeGI GKGCMC 1 cut(s) 517
BalI TGGCCA 1 cut(s) 1254
BauI CACGAG 1 cut(s) 825
BbsI GAAGAC 1 cut(s) 1568
BbvI GCAGC 5 cut(s) 36, 1186, 1258, 1575, 1639
BccI CCATC 4 cut(s) 107, 202, 826, 1334
BceAI ACGGC 2 cut(s) 427, 1131
BciT130I CCWGG 2 cut(s) 643, 1288
BciVI GTATCC 1 cut(s) 168
BcnI CCSGG 1 cut(s) 1593
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 3 cut(s) 122, 915, 975
BfmI CTRYAG 2 cut(s) 492, 1491
BfrI CTTAAG 1 cut(s) 559
BfuAI ACCTGC 1 cut(s) 1024
BfuI GTATCC 1 cut(s) 168
BglII AGATCT 1 cut(s) 532
BisI GCNGC 8 cut(s) 50, 1200, 1272, 1284, 1589, 1634, 1650, 1653
BlsI GCNGC 8 cut(s) 51, 1201, 1273, 1285, 1590, 1635, 1651, 1654
BmcAI AGTACT 1 cut(s) 1316
Bme1390I CCNGG 3 cut(s) 643, 1288, 1593
Bme18I GGWCC 2 cut(s) 1238, 1290
BmeT110I CYCGRG 2 cut(s) 131, 983
BmgT120I GGNCC 2 cut(s) 1238, 1290
BmiI GGNNCC 1 cut(s) 1292
BmrFI CCNGG 3 cut(s) 643, 1288, 1593
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 5 cut(s) 50, 1063, 1363, 1397, 1670
BmuI ACTGGG 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 1568
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 1593
BsaJI CCNNGG 2 cut(s) 465, 1620
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 1 cut(s) 1097
Bse118I RCCGGY 1 cut(s) 1014
Bse1I ACTGG 3 cut(s) 589, 699, 1522
Bse3DI GCAATG 2 cut(s) 730, 1228
BseBI CCWGG 2 cut(s) 643, 1288
BseDI CCNNGG 2 cut(s) 465, 1620
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 1097
BseMI GCAATG 2 cut(s) 730, 1228
BseMII CTCAG 3 cut(s) 603, 650, 1161
BseNI ACTGG 3 cut(s) 589, 699, 1522
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 5 cut(s) 36, 1186, 1258, 1575, 1639
BsgI GTGCAG 2 cut(s) 233, 1565
Bsh1236I CGCG 2 cut(s) 1433, 1510
BshFI GGCC 2 cut(s) 1014, 1254
BsiHKCI CYCGRG 2 cut(s) 131, 983
BsiSI CCGG 3 cut(s) 167, 1015, 1593
BslFI GGGAC 1 cut(s) 1276
BslI CCNNNNNNNGG 1 cut(s) 1097
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 1276
BsnI GGCC 2 cut(s) 1014, 1254
BsoBI CYCGRG 2 cut(s) 131, 983
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
Bsp19I CCATGG 1 cut(s) 1620
BspACI CCGC 6 cut(s) 508, 830, 1284, 1433, 1634, 1650
BspANI GGCC 2 cut(s) 1014, 1254
BspCNI CTCAG 3 cut(s) 604, 651, 1162
BspFNI CGCG 2 cut(s) 1433, 1510
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 1 cut(s) 1292
BspMI ACCTGC 1 cut(s) 1024
BspPI GGATC 7 cut(s) 74, 336, 545, 876, 965, 998, 1447
BspQI GCTCTTC 1 cut(s) 1578
BspTI CTTAAG 1 cut(s) 559
BsrBI CCGCTC 1 cut(s) 1636
BsrDI GCAATG 2 cut(s) 730, 1228
BsrFI RCCGGY 1 cut(s) 1014
BsrI ACTGG 3 cut(s) 589, 699, 1522
BssAI RCCGGY 1 cut(s) 1014
BssECI CCNNGG 2 cut(s) 465, 1620
BssMI GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
BssNAI GTATAC 1 cut(s) 97
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 1 cut(s) 1620
Bst1107I GTATAC 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 825
Bst2UI CCWGG 2 cut(s) 643, 1288
Bst4CI ACNGT 5 cut(s) 457, 577, 672, 1129, 1599
Bst6I CTCTTC 1 cut(s) 1578
BstAFI CTTAAG 1 cut(s) 559
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstC8I GCNNGC 2 cut(s) 462, 1016
BstDEI CTNAG 3 cut(s) 612, 659, 1170
BstDSI CCRYGG 1 cut(s) 1620
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 2 cut(s) 1433, 1510
BstKTI GATC 9 cut(s) 58, 82, 331, 535, 553, 871, 973, 1006, 1455
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
BstMWI GCNNNNNNNGC 5 cut(s) 1205, 1280, 1552, 1658, 1667
BstNI CCWGG 2 cut(s) 643, 1288
BstNSI RCATGY 2 cut(s) 464, 1615
BstSCI CCNGG 3 cut(s) 641, 1286, 1591
BstSFI CTRYAG 2 cut(s) 492, 1491
BstSLI GKGCMC 1 cut(s) 517
BstUI CGCG 2 cut(s) 1433, 1510
BstV1I GCAGC 5 cut(s) 36, 1186, 1258, 1575, 1639
BstV2I GAAGAC 1 cut(s) 1568
BstX2I RGATCY 3 cut(s) 532, 970, 1452
BstYI RGATCY 3 cut(s) 532, 970, 1452
BstZ17I GTATAC 1 cut(s) 97
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 2 cut(s) 1014, 1254
BtgI CCRYGG 1 cut(s) 1620
BtsCI GGATG 1 cut(s) 41
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 3 cut(s) 692, 1135, 1350
BveI ACCTGC 1 cut(s) 1024
Cac8I GCNNGC 2 cut(s) 462, 1016
CciI TCATGA 1 cut(s) 835
Cfr10I RCCGGY 1 cut(s) 1014
Cfr13I GGNCC 2 cut(s) 1238, 1290
CseI GACGC 1 cut(s) 1469
Csp6I GTAC 2 cut(s) 302, 1315
CviAII CATG 5 cut(s) 461, 836, 937, 1612, 1621
CviQI GTAC 2 cut(s) 302, 1315
DdeI CTNAG 3 cut(s) 612, 659, 1170
DpnI GATC 9 cut(s) 57, 81, 330, 534, 552, 870, 972, 1005, 1454
DpnII GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
DraI TTTAAA 2 cut(s) 162, 633
EaeI YGGCCR 2 cut(s) 1012, 1252
Eam1104I CTCTTC 1 cut(s) 1578
EarI CTCTTC 1 cut(s) 1578
Eco130I CCWWGG 1 cut(s) 1620
Eco47I GGWCC 2 cut(s) 1238, 1290
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 2 cut(s) 131, 983
EcoO109I RGGNCCY 1 cut(s) 1290
EcoRI GAATTC 1 cut(s) 1021
EcoRII CCWGG 2 cut(s) 641, 1286
EcoT14I CCWWGG 1 cut(s) 1620
ErhI CCWWGG 1 cut(s) 1620
FaeI CATG 5 cut(s) 464, 839, 940, 1615, 1624
FalI AAGNNNNNCTT 4 cut(s) 324, 356, 538, 570
FaqI GGGAC 1 cut(s) 1276
FatI CATG 5 cut(s) 460, 835, 936, 1611, 1620
FauI CCCGC 1 cut(s) 823
FblI GTMKAC 2 cut(s) 96, 272
Fnu4HI GCNGC 8 cut(s) 50, 1200, 1272, 1284, 1589, 1634, 1650, 1653
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 8 cut(s) 50, 1200, 1272, 1284, 1589, 1634, 1650, 1653
FspBI CTAG 3 cut(s) 122, 915, 975
GluI GCNGC 8 cut(s) 50, 1200, 1272, 1284, 1589, 1634, 1650, 1653
HaeIII GGCC 2 cut(s) 1014, 1254
HapII CCGG 3 cut(s) 167, 1015, 1593
HgaI GACGC 1 cut(s) 1469
Hin1II CATG 5 cut(s) 464, 839, 940, 1615, 1624
HincII GTYRAC 2 cut(s) 904, 1264
HindII GTYRAC 2 cut(s) 904, 1264
HinfI GANTC 6 cut(s) 263, 313, 403, 986, 1166, 1344
HpaI GTTAAC 1 cut(s) 1264
HpaII CCGG 3 cut(s) 167, 1015, 1593
HphI GGTGA 4 cut(s) 445, 814, 1393, 1630
Hpy166II GTNNAC 5 cut(s) 97, 273, 805, 904, 1264
Hpy188I TCNGA 6 cut(s) 318, 367, 408, 677, 1171, 1181
Hpy8I GTNNAC 5 cut(s) 97, 273, 805, 904, 1264
Hpy99I CGWCG 1 cut(s) 1246
HpyAV CCTTC 6 cut(s) 341, 844, 1104, 1181, 1367, 1651
HpyCH4III ACNGT 5 cut(s) 457, 577, 672, 1129, 1599
HpyCH4IV ACGT 3 cut(s) 226, 1244, 1320
HpyCH4V TGCA 8 cut(s) 214, 460, 606, 667, 944, 994, 1160, 1546
HpyF10VI GCNNNNNNNGC 5 cut(s) 1205, 1280, 1552, 1658, 1667
HpyF3I CTNAG 3 cut(s) 612, 659, 1170
HpySE526I ACGT 3 cut(s) 226, 1244, 1320
Hsp92II CATG 5 cut(s) 464, 839, 940, 1615, 1624
KroI GCCGGC 1 cut(s) 1014
KroNI GCCGGC 1 cut(s) 1016
KspAI GTTAAC 1 cut(s) 1264
Kzo9I GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
LguI GCTCTTC 1 cut(s) 1578
Lsp1109I GCAGC 5 cut(s) 36, 1186, 1258, 1575, 1639
LweI GCATC 5 cut(s) 50, 1063, 1363, 1397, 1670
MaeI CTAG 3 cut(s) 122, 915, 975
MaeII ACGT 3 cut(s) 226, 1244, 1320
MaeIII GTNAC 5 cut(s) 64, 373, 1427, 1511, 1607
MalI GATC 9 cut(s) 57, 81, 330, 534, 552, 870, 972, 1005, 1454
MbiI CCGCTC 1 cut(s) 1636
MboI GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
MboII GAAGA 5 cut(s) 118, 813, 1388, 1568, 1595
MflI RGATCY 3 cut(s) 532, 970, 1452
MhlI GDGCHC 1 cut(s) 517
MlsI TGGCCA 1 cut(s) 1254
MluI ACGCGT 1 cut(s) 1508
MluNI TGGCCA 1 cut(s) 1254
MlyI GAGTC 3 cut(s) 307, 397, 995
MmeI TCCRAC 3 cut(s) 1159, 1326, 1399
Mox20I TGGCCA 1 cut(s) 1254
MroNI GCCGGC 1 cut(s) 1014
MscI TGGCCA 1 cut(s) 1254
MseI TTAA 7 cut(s) 161, 395, 501, 560, 632, 930, 1263
Msp20I TGGCCA 1 cut(s) 1254
MspA1I CMGCKG 2 cut(s) 510, 1652
MspCI CTTAAG 1 cut(s) 559
MspI CCGG 3 cut(s) 167, 1015, 1593
MspR9I CCNGG 3 cut(s) 643, 1288, 1593
MvaI CCWGG 2 cut(s) 643, 1288
MvnI CGCG 2 cut(s) 1433, 1510
MwoI GCNNNNNNNGC 5 cut(s) 1205, 1280, 1552, 1658, 1667
NaeI GCCGGC 1 cut(s) 1016
NciI CCSGG 1 cut(s) 1593
NcoI CCATGG 1 cut(s) 1620
NdeII GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
NgoMIV GCCGGC 1 cut(s) 1014
NlaIII CATG 5 cut(s) 464, 839, 940, 1615, 1624
NlaIV GGNNCC 1 cut(s) 1292
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 3 cut(s) 64, 373, 1511
NspI RCATGY 2 cut(s) 464, 1615
PaeI GCATGC 1 cut(s) 464
PagI TCATGA 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 1578
PdiI GCCGGC 1 cut(s) 1016
PfeI GAWTC 3 cut(s) 263, 1166, 1344
PflFI GACNNNGTC 1 cut(s) 1517
PkrI GCNGC 8 cut(s) 51, 1201, 1273, 1285, 1590, 1635, 1651, 1654
PleI GAGTC 3 cut(s) 307, 397, 994
PpsI GAGTC 3 cut(s) 307, 397, 994
PpuMI RGGWCCY 1 cut(s) 1290
PsiI TTATAA 2 cut(s) 1040, 1334
Psp5II RGGWCCY 1 cut(s) 1290
Psp6I CCWGG 2 cut(s) 641, 1286
PspGI CCWGG 2 cut(s) 641, 1286
PspN4I GGNNCC 1 cut(s) 1292
PspPI GGNCC 2 cut(s) 1238, 1290
PspPPI RGGWCCY 1 cut(s) 1290
PsuI RGATCY 3 cut(s) 532, 970, 1452
PsyI GACNNNGTC 1 cut(s) 1517
RsaI GTAC 2 cut(s) 303, 1316
RsaNI GTAC 2 cut(s) 302, 1315
SapI GCTCTTC 1 cut(s) 1578
SaqAI TTAA 7 cut(s) 161, 395, 501, 560, 632, 930, 1263
SatI GCNGC 8 cut(s) 50, 1200, 1272, 1284, 1589, 1634, 1650, 1653
Sau3AI GATC 9 cut(s) 55, 79, 328, 532, 550, 868, 970, 1003, 1452
Sau96I GGNCC 2 cut(s) 1238, 1290
ScaI AGTACT 1 cut(s) 1316
SchI GAGTC 3 cut(s) 307, 397, 995
ScrFI CCNGG 3 cut(s) 643, 1288, 1593
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 5 cut(s) 50, 1063, 1363, 1397, 1670
SfcI CTRYAG 2 cut(s) 492, 1491
SinI GGWCC 2 cut(s) 1238, 1290
SmiI ATTTAAAT 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 101, 559
SmoI CTYRAG 2 cut(s) 101, 559
SphI GCATGC 1 cut(s) 464
SsiI CCGC 6 cut(s) 508, 830, 1284, 1433, 1634, 1650
SspI AATATT 1 cut(s) 27
SspMI CTAG 3 cut(s) 122, 915, 975
StyD4I CCNGG 3 cut(s) 641, 1286, 1591
StyI CCWWGG 1 cut(s) 1620
SwaI ATTTAAAT 1 cut(s) 162
TaaI ACNGT 5 cut(s) 457, 577, 672, 1129, 1599
TaiI ACGT 3 cut(s) 229, 1247, 1323
TaqI TCGA 2 cut(s) 204, 388
TaqII GACCGA 1 cut(s) 1255
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 3 cut(s) 1286, 1636, 1652
TfiI GAWTC 3 cut(s) 263, 1166, 1344
Tru1I TTAA 7 cut(s) 161, 395, 501, 560, 632, 930, 1263
Tru9I TTAA 7 cut(s) 161, 395, 501, 560, 632, 930, 1263
TscAI CASTG 3 cut(s) 699, 1142, 1357
TseFI GTSAC 3 cut(s) 64, 373, 1511
TseI GCWGC 5 cut(s) 49, 1199, 1271, 1588, 1652
Tsp45I GTSAC 3 cut(s) 64, 373, 1511
TspDTI ATGAA 2 cut(s) 852, 925
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 3 cut(s) 699, 1142, 1357
Tth111I GACNNNGTC 1 cut(s) 1517
Vha464I CTTAAG 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 1238, 1290
XapI RAATTY 4 cut(s) 217, 319, 503, 1021
XceI RCATGY 2 cut(s) 464, 1615
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmiI GTMKAC 2 cut(s) 96, 272
XspI CTAG 3 cut(s) 122, 915, 975
ZrmI AGTACT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.