MD12G1228200.v1.1

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
30351221 .. 30357589
6369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1228200.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGGGAGGATGCTTTTCGGACGTGAGGGGAGGCAAGGACGCCGTGGGCGGGGCCCTGCCAAGGCCCACCGAACCGAACGCCAAGAACGACGGCGCTCACAACGATGCCATTGAATTCTTTTACAGGGCGCATGGTGCTTACCCGCTTTTTACGCAACTCGAGTTATCCTTTTCAGCATCCAAGCTGCTTGATCTTGATGTCACTTCAAAGAGTGATCCTATGGTGGTTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTCGGGAGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGATGGTGCAGAATTTGATATTCCGTGTCTACGATGTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCAAGAGTTTCTTGGAGAAGGCAGTTGTGTTCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTTAACATTGACTCTGAACGGCAAAAACGGGCTTGGAGGTTTGAGAAACTTTGGAACACTCACCGTGCATGCCGAGGAAACTATTGCTTCTACGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTCTTTTCTCTAAAAGCGTACGCATTTCAAAGAAGCTTCGATCCTTTCTTAAGAATATCAAGAACTGTTGAGACTGGGGGTTCTATTCCAATTTGCAAGACTGAGGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACACCCCACTGGTTTTTGAGTGTTTTGATTTCAACAGCAATGGCAACCACATTCTTATTGGGAAACTCCAAAAATCAGTAGCAGACCTTGAAAAACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAAGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACGGGTTCAAATGGAGATCCTAGAAAGCCCGAGTCTTTGCACTATATTGATCCTTATGGCCGGCTGAATTCTTACCAGCAGGTTATAACAGAAGTAGGACAAGTCATTCAGTTCTATGATGCTGATAAGAGTTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCTACGCAATGTTGCTCTGTCAGGACCGACGTTGTTTGGCCAAGTGGTTAACAGGGCTGCTGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAACAAGTACTACGTCTTGCTCATTATAACAGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAACGCGGATTTCAAACAAATGGAGATCCTTGACGCTGACAACGGGCAACGATTAGAAAGTTCTACAGGTCGCATAGCTACGCGTGACATTGTCCAGTTCGTTCCAATGAGAGATGTGCAGAGTGGGCAGATTTCTGTTGTTCAAGGTCTTCTGGAAGAGCTGCCCGGACAGTTTTTGAGTTACATGCGTTCCATGGACATCAAGCCGCTCACCTTCAATGCAGCTGCCCAAGCATCACCTGCCTTAGAAATTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

587

Amino Acids

64.67

Weight (kDa)

5.48

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 58 - 162 5.5e-12 C2 domain
C2 PF00168 203 - 299 2.5e-09 C2 domain
Copine PF07002 370 - 570 1.2e-68 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1160, 1409
AarI CACCTGC 1 cut(s) 1750
Acc36I ACCTGC 2 cut(s) 1144, 1750
AccBSI CCGCTC 1 cut(s) 1711
AccI GTMKAC 2 cut(s) 231, 369
AccII CGCG 2 cut(s) 1508, 1585
AciI CCGC 7 cut(s) 48, 143, 604, 950, 1359, 1508, 1709
AclWI GGATC 7 cut(s) 209, 432, 665, 996, 1085, 1118, 1522
AcoI YGGCCR 2 cut(s) 1132, 1327
AcsI RAATTY 6 cut(s) 113, 352, 415, 599, 1141, 1752
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 399, 650, 1391
AfiI CCNNNNNNNGG 3 cut(s) 48, 60, 1217
AflII CTTAAG 1 cut(s) 679
AflIII ACRYGT 1 cut(s) 1583
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 761, 1361
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 7 cut(s) 209, 432, 665, 996, 1085, 1118, 1522
Ama87I CYCGRG 3 cut(s) 158, 266, 1103
AoxI GGCC 4 cut(s) 51, 62, 1132, 1327
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 6 cut(s) 184, 1274, 1346, 1663, 1724, 1727
ApoI RAATTY 6 cut(s) 113, 352, 415, 599, 1141, 1752
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
AspLEI GCGC 2 cut(s) 95, 130
AspS9I GGNCC 5 cut(s) 51, 52, 63, 1313, 1365
AsuC2I CCSGG 1 cut(s) 1668
AsuHPI GGTGA 5 cut(s) 541, 934, 1468, 1705, 1731
AvaI CYCGRG 3 cut(s) 158, 266, 1103
AvaII GGWCC 2 cut(s) 1313, 1365
BaeGI GKGCMC 2 cut(s) 55, 613
BalI TGGCCA 1 cut(s) 1329
BanII GRGCYC 1 cut(s) 55
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 1 cut(s) 1643
BbvI GCAGC 6 cut(s) 171, 1261, 1333, 1650, 1714, 1736
BccI CCATC 4 cut(s) 242, 337, 946, 1409
BceAI ACGGC 4 cut(s) 26, 106, 523, 1251
BciT130I CCWGG 2 cut(s) 763, 1363
BciVI GTATCC 1 cut(s) 303
BcnI CCSGG 1 cut(s) 1668
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 3 cut(s) 257, 1035, 1095
BfmI CTRYAG 2 cut(s) 588, 1566
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 679
BfuAI ACCTGC 2 cut(s) 1144, 1750
BfuI GTATCC 1 cut(s) 303
BglII AGATCT 1 cut(s) 628
BisI GCNGC 8 cut(s) 185, 1275, 1347, 1359, 1664, 1709, 1725, 1728
BlsI GCNGC 8 cut(s) 186, 1276, 1348, 1360, 1665, 1710, 1726, 1729
BmcAI AGTACT 1 cut(s) 1391
Bme1390I CCNGG 3 cut(s) 763, 1363, 1668
Bme18I GGWCC 2 cut(s) 1313, 1365
BmeT110I CYCGRG 3 cut(s) 158, 266, 1103
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 5 cut(s) 51, 52, 63, 1313, 1365
BmiI GGNNCC 3 cut(s) 52, 53, 1367
BmrFI CCNGG 3 cut(s) 763, 1363, 1668
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 6 cut(s) 94, 185, 1183, 1438, 1472, 1745
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 1643
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 1668
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 42, 59, 561, 1695
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 60, 1217
Bse118I RCCGGY 1 cut(s) 1134
Bse1I ACTGG 3 cut(s) 709, 819, 1597
Bse3DI GCAATG 2 cut(s) 850, 1303
BseBI CCWGG 2 cut(s) 763, 1363
BseDI CCNNGG 4 cut(s) 42, 59, 561, 1695
BseGI GGATG 2 cut(s) 14, 176
BseLI CCNNNNNNNGG 3 cut(s) 48, 60, 1217
BseMI GCAATG 2 cut(s) 850, 1303
BseMII CTCAG 2 cut(s) 723, 770
BseNI ACTGG 3 cut(s) 709, 819, 1597
BseSI GKGCMC 2 cut(s) 55, 613
BseXI GCAGC 6 cut(s) 171, 1261, 1333, 1650, 1714, 1736
BsgI GTGCAG 2 cut(s) 368, 1640
Bsh1236I CGCG 2 cut(s) 1508, 1585
BshFI GGCC 4 cut(s) 53, 64, 1134, 1329
BsiHKCI CYCGRG 3 cut(s) 158, 266, 1103
BsiSI CCGG 3 cut(s) 302, 1135, 1668
BsiWI CGTACG 1 cut(s) 648
BslFI GGGAC 1 cut(s) 1351
BslI CCNNNNNNNGG 3 cut(s) 48, 60, 1217
BsmAI GTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 1351
BsnI GGCC 4 cut(s) 53, 64, 1134, 1329
BsoBI CYCGRG 3 cut(s) 158, 266, 1103
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 55, 613
Bsp143I GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
Bsp19I CCATGG 1 cut(s) 1695
BspACI CCGC 7 cut(s) 48, 143, 604, 950, 1359, 1508, 1709
BspANI GGCC 4 cut(s) 53, 64, 1134, 1329
BspCNI CTCAG 2 cut(s) 724, 771
BspFNI CGCG 2 cut(s) 1508, 1585
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 3 cut(s) 52, 53, 1367
BspMI ACCTGC 2 cut(s) 1144, 1750
BspPI GGATC 7 cut(s) 209, 432, 665, 996, 1085, 1118, 1522
BspQI GCTCTTC 1 cut(s) 1653
BspTI CTTAAG 1 cut(s) 679
BsrBI CCGCTC 1 cut(s) 1711
BsrDI GCAATG 2 cut(s) 850, 1303
BsrFI RCCGGY 1 cut(s) 1134
BsrI ACTGG 3 cut(s) 709, 819, 1597
BssAI RCCGGY 1 cut(s) 1134
BssECI CCNNGG 4 cut(s) 42, 59, 561, 1695
BssMI GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
BssNAI GTATAC 1 cut(s) 232
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 945
BssT1I CCWWGG 2 cut(s) 59, 1695
Bst1107I GTATAC 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 2 cut(s) 763, 1363
Bst4CI ACNGT 5 cut(s) 553, 697, 792, 1249, 1674
Bst6I CTCTTC 1 cut(s) 1653
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 1742
BstC8I GCNNGC 2 cut(s) 558, 1136
BstDEI CTNAG 3 cut(s) 732, 779, 1747
BstDSI CCRYGG 2 cut(s) 42, 1695
BstF5I GGATG 2 cut(s) 14, 176
BstFNI CGCG 2 cut(s) 1508, 1585
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 130
BstKTI GATC 9 cut(s) 193, 217, 427, 631, 673, 991, 1093, 1126, 1530
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
BstMWI GCNNNNNNNGC 7 cut(s) 134, 151, 1280, 1355, 1627, 1733, 1742
BstNI CCWGG 2 cut(s) 763, 1363
BstNSI RCATGY 2 cut(s) 560, 1690
BstSCI CCNGG 3 cut(s) 761, 1361, 1666
BstSFI CTRYAG 2 cut(s) 588, 1566
BstSLI GKGCMC 2 cut(s) 55, 613
BstUI CGCG 2 cut(s) 1508, 1585
BstV1I GCAGC 6 cut(s) 171, 1261, 1333, 1650, 1714, 1736
BstV2I GAAGAC 1 cut(s) 1643
BstX2I RGATCY 3 cut(s) 628, 1090, 1527
BstYI RGATCY 3 cut(s) 628, 1090, 1527
BstZ17I GTATAC 1 cut(s) 232
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 4 cut(s) 53, 64, 1134, 1329
BtgI CCRYGG 2 cut(s) 42, 1695
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 14, 176
BtsIMutI CAGTG 2 cut(s) 812, 1425
BveI ACCTGC 2 cut(s) 1144, 1750
Cac8I GCNNGC 2 cut(s) 558, 1136
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 95, 130
Cfr10I RCCGGY 1 cut(s) 1134
Cfr13I GGNCC 5 cut(s) 51, 52, 63, 1313, 1365
CseI GACGC 2 cut(s) 47, 1544
Csp6I GTAC 3 cut(s) 398, 649, 1390
CviAII CATG 6 cut(s) 131, 557, 956, 1057, 1687, 1696
CviQI GTAC 3 cut(s) 398, 649, 1390
DdeI CTNAG 3 cut(s) 732, 779, 1747
DpnI GATC 9 cut(s) 192, 216, 426, 630, 672, 990, 1092, 1125, 1529
DpnII GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
DraI TTTAAA 2 cut(s) 297, 753
EaeI YGGCCR 2 cut(s) 1132, 1327
Eam1104I CTCTTC 1 cut(s) 1653
EarI CTCTTC 1 cut(s) 1653
Eco130I CCWWGG 2 cut(s) 59, 1695
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 1313, 1365
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 3 cut(s) 158, 266, 1103
EcoO109I RGGNCCY 3 cut(s) 51, 52, 1365
EcoRI GAATTC 2 cut(s) 113, 1141
EcoRII CCWGG 2 cut(s) 761, 1361
EcoT14I CCWWGG 2 cut(s) 59, 1695
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 59, 1695
FaeI CATG 6 cut(s) 134, 560, 959, 1060, 1690, 1699
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 1351
FatI CATG 6 cut(s) 130, 556, 955, 1056, 1686, 1695
FauI CCCGC 3 cut(s) 41, 150, 943
FblI GTMKAC 2 cut(s) 231, 369
Fnu4HI GCNGC 8 cut(s) 185, 1275, 1347, 1359, 1664, 1709, 1725, 1728
FokI GGATG 2 cut(s) 21, 163
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 8 cut(s) 185, 1275, 1347, 1359, 1664, 1709, 1725, 1728
FspBI CTAG 3 cut(s) 257, 1035, 1095
GlaI GCGC 2 cut(s) 94, 129
GluI GCNGC 8 cut(s) 185, 1275, 1347, 1359, 1664, 1709, 1725, 1728
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 53, 64, 1134, 1329
HapII CCGG 3 cut(s) 302, 1135, 1668
HgaI GACGC 2 cut(s) 47, 1544
HhaI GCGC 2 cut(s) 95, 130
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 134, 560, 959, 1060, 1690, 1699
Hin6I GCGC 2 cut(s) 93, 128
HinP1I GCGC 2 cut(s) 93, 128
HincII GTYRAC 2 cut(s) 1024, 1339
HindII GTYRAC 2 cut(s) 1024, 1339
HindIII AAGCTT 1 cut(s) 664
HinfI GANTC 4 cut(s) 409, 499, 1106, 1419
HpaI GTTAAC 1 cut(s) 1339
HpaII CCGG 3 cut(s) 302, 1135, 1668
HphI GGTGA 5 cut(s) 541, 934, 1468, 1705, 1731
Hpy166II GTNNAC 5 cut(s) 232, 370, 925, 1024, 1339
Hpy188I TCNGA 6 cut(s) 19, 414, 463, 504, 797, 1256
Hpy8I GTNNAC 5 cut(s) 232, 370, 925, 1024, 1339
Hpy99I CGWCG 2 cut(s) 92, 1321
HpyAV CCTTC 6 cut(s) 437, 964, 1224, 1256, 1442, 1726
HpyCH4III ACNGT 5 cut(s) 553, 697, 792, 1249, 1674
HpyCH4IV ACGT 3 cut(s) 21, 1319, 1395
HpyCH4V TGCA 8 cut(s) 349, 556, 726, 787, 1064, 1114, 1621, 1724
HpyF10VI GCNNNNNNNGC 7 cut(s) 134, 151, 1280, 1355, 1627, 1733, 1742
HpyF3I CTNAG 3 cut(s) 732, 779, 1747
HpySE526I ACGT 3 cut(s) 21, 1319, 1395
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 134, 560, 959, 1060, 1690, 1699
HspAI GCGC 2 cut(s) 93, 128
KroI GCCGGC 1 cut(s) 1134
KroNI GCCGGC 1 cut(s) 1136
KspAI GTTAAC 1 cut(s) 1339
Kzo9I GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
LguI GCTCTTC 1 cut(s) 1653
Lsp1109I GCAGC 6 cut(s) 171, 1261, 1333, 1650, 1714, 1736
LweI GCATC 6 cut(s) 94, 185, 1183, 1438, 1472, 1745
MaeI CTAG 3 cut(s) 257, 1035, 1095
MaeII ACGT 3 cut(s) 21, 1319, 1395
MaeIII GTNAC 5 cut(s) 199, 469, 1502, 1586, 1682
MalI GATC 9 cut(s) 192, 216, 426, 630, 672, 990, 1092, 1125, 1529
MbiI CCGCTC 1 cut(s) 1711
MboI GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
MboII GAAGA 5 cut(s) 253, 933, 1463, 1643, 1670
MflI RGATCY 3 cut(s) 628, 1090, 1527
MhlI GDGCHC 2 cut(s) 55, 613
MlsI TGGCCA 1 cut(s) 1329
MluI ACGCGT 1 cut(s) 1583
MluNI TGGCCA 1 cut(s) 1329
MlyI GAGTC 3 cut(s) 403, 493, 1115
MmeI TCCRAC 3 cut(s) 1234, 1401, 1474
Mox20I TGGCCA 1 cut(s) 1329
MroNI GCCGGC 1 cut(s) 1134
MscI TGGCCA 1 cut(s) 1329
MseI TTAA 7 cut(s) 296, 491, 597, 680, 752, 1050, 1338
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 1329
MspA1I CMGCKG 2 cut(s) 606, 1727
MspCI CTTAAG 1 cut(s) 679
MspI CCGG 3 cut(s) 302, 1135, 1668
MspR9I CCNGG 3 cut(s) 763, 1363, 1668
MvaI CCWGG 2 cut(s) 763, 1363
MvnI CGCG 2 cut(s) 1508, 1585
MwoI GCNNNNNNNGC 7 cut(s) 134, 151, 1280, 1355, 1627, 1733, 1742
NaeI GCCGGC 1 cut(s) 1136
NciI CCSGG 1 cut(s) 1668
NcoI CCATGG 1 cut(s) 1695
NdeII GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
NgoMIV GCCGGC 1 cut(s) 1134
NlaIII CATG 6 cut(s) 134, 560, 959, 1060, 1690, 1699
NlaIV GGNNCC 3 cut(s) 52, 53, 1367
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 3 cut(s) 199, 469, 1586
NspI RCATGY 2 cut(s) 560, 1690
PaeI GCATGC 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 955
PaqCI CACCTGC 1 cut(s) 1750
PciSI GCTCTTC 1 cut(s) 1653
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 1136
PfeI GAWTC 1 cut(s) 1419
Pfl23II CGTACG 1 cut(s) 648
PflFI GACNNNGTC 1 cut(s) 1592
PkrI GCNGC 8 cut(s) 186, 1276, 1348, 1360, 1665, 1710, 1726, 1729
PleI GAGTC 3 cut(s) 403, 493, 1114
PpsI GAGTC 3 cut(s) 403, 493, 1114
PpuMI RGGWCCY 1 cut(s) 1365
PsiI TTATAA 2 cut(s) 1160, 1409
Psp5II RGGWCCY 1 cut(s) 1365
Psp6I CCWGG 2 cut(s) 761, 1361
PspGI CCWGG 2 cut(s) 761, 1361
PspLI CGTACG 1 cut(s) 648
PspN4I GGNNCC 3 cut(s) 52, 53, 1367
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 5 cut(s) 51, 52, 63, 1313, 1365
PspPPI RGGWCCY 1 cut(s) 1365
PspXI VCTCGAGB 1 cut(s) 158
PsuI RGATCY 3 cut(s) 628, 1090, 1527
PsyI GACNNNGTC 1 cut(s) 1592
PvuII CAGCTG 1 cut(s) 1727
RsaI GTAC 3 cut(s) 399, 650, 1391
RsaNI GTAC 3 cut(s) 398, 649, 1390
RseI CAYNNNNRTG 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 1653
SaqAI TTAA 7 cut(s) 296, 491, 597, 680, 752, 1050, 1338
SatI GCNGC 8 cut(s) 185, 1275, 1347, 1359, 1664, 1709, 1725, 1728
Sau3AI GATC 9 cut(s) 190, 214, 424, 628, 670, 988, 1090, 1123, 1527
Sau96I GGNCC 5 cut(s) 51, 52, 63, 1313, 1365
ScaI AGTACT 1 cut(s) 1391
SchI GAGTC 3 cut(s) 403, 493, 1115
ScrFI CCNGG 3 cut(s) 763, 1363, 1668
SduI GDGCHC 2 cut(s) 55, 613
SfaNI GCATC 6 cut(s) 94, 185, 1183, 1438, 1472, 1745
SfcI CTRYAG 2 cut(s) 588, 1566
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 2 cut(s) 1313, 1365
SlaI CTCGAG 1 cut(s) 158
SmiI ATTTAAAT 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 158, 236, 679
SmoI CTYRAG 3 cut(s) 158, 236, 679
SphI GCATGC 1 cut(s) 560
SsiI CCGC 7 cut(s) 48, 143, 604, 950, 1359, 1508, 1709
SspMI CTAG 3 cut(s) 257, 1035, 1095
StyD4I CCNGG 3 cut(s) 761, 1361, 1666
StyI CCWWGG 2 cut(s) 59, 1695
SwaI ATTTAAAT 1 cut(s) 297
TaaI ACNGT 5 cut(s) 553, 697, 792, 1249, 1674
TaiI ACGT 3 cut(s) 24, 1322, 1398
TaqI TCGA 4 cut(s) 159, 339, 484, 669
TaqII GACCGA 1 cut(s) 1330
TatI WGTACW 1 cut(s) 1389
TauI GCSGC 2 cut(s) 1361, 1711
TfiI GAWTC 1 cut(s) 1419
Tru1I TTAA 7 cut(s) 296, 491, 597, 680, 752, 1050, 1338
Tru9I TTAA 7 cut(s) 296, 491, 597, 680, 752, 1050, 1338
TscAI CASTG 2 cut(s) 819, 1432
TseFI GTSAC 3 cut(s) 199, 469, 1586
TseI GCWGC 6 cut(s) 184, 1274, 1346, 1663, 1724, 1727
Tsp45I GTSAC 3 cut(s) 199, 469, 1586
TspDTI ATGAA 2 cut(s) 972, 1045
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 819, 1432
Tth111I GACNNNGTC 1 cut(s) 1592
Vha464I CTTAAG 1 cut(s) 679
VpaK11BI GGWCC 2 cut(s) 1313, 1365
XapI RAATTY 6 cut(s) 113, 352, 415, 599, 1141, 1752
XceI RCATGY 2 cut(s) 560, 1690
XcmI CCANNNNNNNNNTGG 1 cut(s) 860
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 231, 369
XspI CTAG 3 cut(s) 257, 1035, 1095
ZrmI AGTACT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.