Rorug02G0648300

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000862
Physical Location & Seq
Forward (+)
75776372 .. 75782634
6263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0648300.1

Sequence Viewer

Length: 1638 bp
ATGATGAGGAGAAGGAGACGAGTTTCTGTAGGGTCAATTCTGATATTCGTTGCAATTTTTGCGGGAATCGGATTGCTGCTGATTCTCAACTTAAGACCTGTCCATGATGATTCTCCAACGAGCCGCGATTTCCCTGTTGAAATTCACGACTCGGAGACTCCGACTCGGAGCAGCGAGAGTAAGAGTAAGAGAAAGAGAAAATCGAGCTCGTGTGCTTCGGTGGAAGAGATGGGAAAGGAGTTTGAAGGAGGAGGGTTTTGGGATGAAAGTTTCAGAGTGAGGAAACTTATTCAAAATCACTTTGAATTGAATGGTGCTTTGAGAGTGCGGAATCTTCCTCCACAACAGTTTTGCCAGCATGGTTTTGTGCTTGGTAAATCAGCAGAGGCAGGCTTCGGGAATGAAATGTACAAGATCTTAAGTGGTGCAGCACTGAGTGTGATGTTAAACCGGTCCCTCATTATTGGGCAAACCAGGGGAAAATTCCCTTTTGGGGATTACATTTCTTATTCCAACCTTTCTTTTACAATGAAAGAAATCAAGCATCTGTGGAGACTTAATAAATGTGTAAACAAGTACGGAAGGCAACTGATTATGAGGACTGATGACTTTGAGAAACCAATGAAGACAAATGTCCTGTGTAGCGACTGGAGGAGATGGAAGCAACCTATTATATGGTTCCAAGGTACAAATGACGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATACATCCAGAGATGAGGAAGGCTGCTTCTAATTTATTTGGAAAGCCAGAGGTTCTCCATTCTCGGCCTAATGTATTTGGAGAGCTGATGAGAGTGCTTATATCTCCTTCTGAAGATGTTGAGCAAGCAATCAATTGGGTTATCGGTGGAGTGGATCCTGATATTTCTCTGCACATGCGAATGCTCATGAATAGGTCGGTGAGAGCTGCACAGGCAGCACTAAACTGCATCAGAAAAGCTGTGCGCAATCTTGGTAAAGCCTCAAGGCCCAGGGTGGTCTTAGTGTCAGATACCCCCTCTCTTATAAAAAGTATTACACCGAATATAAGCAAGTTTGCAGAGGTAATTCATTTTGATTACGAACTTTTCAAAGGAAATATATCTGATGGCGGTAAAAGATTGCGAAGTTTAGAATTTAGAGCGAAAGATTGGGGCCCAGCACCCAGATGGGTCGCCTTTGTGGACTTCTTTCTTGCATCACGGGCAAAACATGCTGTTGTGTCTGGTGCTCACAGGCGTGTTGGGACTACATATGCTCAGTTAATTGCAGCACTGGCTGCAGCAAACAGTCTAGGGGACAGTCCAACTGGTTCGAATTTTTCGTTCTTAAGCAGCTTTCAAAGTGATTTGTTGAGAGAAGGTTTAAGATTTCAGATTGGTTGGGGACATGTGTGGAACAGATTTGCAGGTCCCCTTAGTTGTCACAACCAGCATAACCAATGTGCTTTCACACCACTTCTCCCTCCTGCATGGTGGGATGGTCTTTGGCAATCTCCACTAACAAGGGATATTAATAGGTTGGCAGACTACGGCATCCAGTTAACCGGTTTTGGCACCATTGATGAAACACATCTTCAGTCTTTTTGTAGCTCAAAGAAAGTTGTTGTGAAAACTATTCCGTACATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

545

Amino Acids

61.54

Weight (kDa)

9.93

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 67 - 541 3e-263 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
Acc16I TGCGCA 1 cut(s) 971
Acc36I ACCTGC 1 cut(s) 1403
AccB1I GGYRCC 1 cut(s) 1559
AccII CGCG 1 cut(s) 126
AciI CCGC 4 cut(s) 62, 124, 328, 1116
AclWI GGATC 2 cut(s) 875, 888
AcsI RAATTY 4 cut(s) 141, 482, 1139, 1321
AcuI CTGAAG 2 cut(s) 858, 1565
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 4 cut(s) 410, 578, 688, 1628
AfiI CCNNNNNNNGG 1 cut(s) 493
AflII CTTAAG 3 cut(s) 91, 418, 1333
AflIII ACRYGT 1 cut(s) 1393
AgeI ACCGGT 2 cut(s) 450, 1550
AgsI TTSAA 8 cut(s) 140, 245, 293, 305, 310, 721, 1096, 1346
AjnI CCWGG 2 cut(s) 473, 995
AleI CACNNNNGTG 1 cut(s) 1242
AluBI AGCT 6 cut(s) 207, 811, 932, 965, 1341, 1596
AluI AGCT 6 cut(s) 207, 811, 932, 965, 1341, 1596
Alw21I GWGCWC 2 cut(s) 209, 1237
Alw26I GTCTC 3 cut(s) 10, 149, 547
AlwI GGATC 2 cut(s) 875, 888
AoxI GGCC 3 cut(s) 791, 992, 1159
ApaI GGGCCC 1 cut(s) 1163
ApoI RAATTY 4 cut(s) 141, 482, 1139, 1321
AseI ATTAAT 1 cut(s) 1518
AsiGI ACCGGT 2 cut(s) 450, 1550
AspLEI GCGC 1 cut(s) 972
AspS9I GGNCC 5 cut(s) 453, 993, 1159, 1160, 1415
AsuHPI GGTGA 1 cut(s) 937
AsuII TTCGAA 1 cut(s) 1319
AvaII GGWCC 2 cut(s) 453, 1415
BaeGI GKGCMC 1 cut(s) 1163
BamHI GGATCC 1 cut(s) 880
BanI GGYRCC 1 cut(s) 1559
BanII GRGCYC 2 cut(s) 209, 1163
BauI CACGAG 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 209, 1237
BccI CCATC 5 cut(s) 223, 651, 1106, 1167, 1478
BceAI ACGGC 1 cut(s) 1552
BciT130I CCWGG 2 cut(s) 475, 997
BcoDI GTCTC 3 cut(s) 10, 149, 547
BfaI CTAG 1 cut(s) 1298
BfmI CTRYAG 2 cut(s) 27, 1284
BfrI CTTAAG 3 cut(s) 91, 418, 1333
BfuAI ACCTGC 1 cut(s) 1403
BglII AGATCT 1 cut(s) 414
Bme1390I CCNGG 2 cut(s) 475, 997
Bme18I GGWCC 2 cut(s) 453, 1415
BmgT120I GGNCC 5 cut(s) 453, 993, 1159, 1160, 1415
BmiI GGNNCC 7 cut(s) 455, 680, 882, 1160, 1161, 1417, 1561
BmrFI CCNGG 2 cut(s) 475, 997
BmrI ACTGGG 1 cut(s) 703
BmsI GCATC 4 cut(s) 553, 963, 1211, 1548
BmuI ACTGGG 1 cut(s) 703
BoxI GACNNNNGTC 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 1319
BpuEI CTTGAG 1 cut(s) 973
BsaJI CCNNGG 4 cut(s) 474, 682, 995, 996
BsaWI WCCGGW 2 cut(s) 450, 1550
BsaXI ACNNNNNCTCC 2 cut(s) 1449, 1479
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 2 cut(s) 450, 1550
Bse1I ACTGG 5 cut(s) 653, 709, 1284, 1318, 1543
BseBI CCWGG 2 cut(s) 475, 997
BseDI CCNNGG 4 cut(s) 474, 682, 995, 996
BseGI GGATG 4 cut(s) 268, 730, 1489, 1539
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMII CTCAG 2 cut(s) 425, 1277
BseNI ACTGG 5 cut(s) 653, 709, 1284, 1318, 1543
BseRI GAGGAG 3 cut(s) 22, 264, 667
BseSI GKGCMC 1 cut(s) 1163
BseYI CCCAGC 1 cut(s) 1162
BsgI GTGCAG 3 cut(s) 447, 881, 918
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 793, 994, 1161
BshNI GGYRCC 1 cut(s) 1559
BshTI ACCGGT 2 cut(s) 450, 1550
BsiHKAI GWGCWC 2 cut(s) 209, 1237
BsiSI CCGG 2 cut(s) 451, 1551
BslFI GGGAC 5 cut(s) 439, 1264, 1316, 1401, 1404
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 3 cut(s) 10, 149, 547
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 5 cut(s) 439, 1264, 1316, 1401, 1404
BsmI GAATGC 1 cut(s) 912
BsnI GGCC 3 cut(s) 793, 994, 1161
Bsp119I TTCGAA 1 cut(s) 1319
Bsp120I GGGCCC 1 cut(s) 1159
Bsp1286I GDGCHC 3 cut(s) 209, 1163, 1237
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 2 cut(s) 414, 880
BspACI CCGC 4 cut(s) 62, 124, 328, 1116
BspANI GGCC 3 cut(s) 793, 994, 1161
BspCNI CTCAG 2 cut(s) 426, 1276
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 7 cut(s) 455, 680, 882, 1160, 1161, 1417, 1561
BspMAI CTGCAG 1 cut(s) 1288
BspMI ACCTGC 1 cut(s) 1403
BspPI GGATC 2 cut(s) 875, 888
BspT104I TTCGAA 1 cut(s) 1319
BspT107I GGYRCC 1 cut(s) 1559
BspTI CTTAAG 3 cut(s) 91, 418, 1333
BsrFI RCCGGY 2 cut(s) 450, 1550
BsrGI TGTACA 1 cut(s) 408
BsrI ACTGG 5 cut(s) 653, 709, 1284, 1318, 1543
BssAI RCCGGY 2 cut(s) 450, 1550
BssECI CCNNGG 4 cut(s) 474, 682, 995, 996
BssMI GATC 2 cut(s) 414, 880
BssSI CACGAG 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 682
Bst2BI CACGAG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 475, 997
Bst4CI ACNGT 3 cut(s) 348, 1295, 1307
Bst6I CTCTTC 1 cut(s) 219
BstAFI CTTAAG 3 cut(s) 91, 418, 1333
BstAPI GCANNNNNTGC 3 cut(s) 59, 1217, 1283
BstAUI TGTACA 1 cut(s) 408
BstBI TTCGAA 1 cut(s) 1319
BstC8I GCNNGC 3 cut(s) 356, 391, 852
BstDEI CTNAG 4 cut(s) 434, 1006, 1263, 1421
BstF5I GGATG 4 cut(s) 268, 730, 1489, 1539
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 972
BstKTI GATC 2 cut(s) 417, 883
BstMAI GTCTC 3 cut(s) 10, 149, 547
BstMBI GATC 2 cut(s) 414, 880
BstMWI GCNNNNNNNGC 8 cut(s) 59, 704, 938, 941, 1208, 1217, 1280, 1283
BstNI CCWGG 2 cut(s) 475, 997
BstNSI RCATGY 3 cut(s) 904, 1220, 1397
BstPAI GACNNNNGTC 1 cut(s) 632
BstSCI CCNGG 2 cut(s) 473, 995
BstSFI CTRYAG 2 cut(s) 27, 1284
BstSLI GKGCMC 1 cut(s) 1163
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 2 cut(s) 414, 880
BstYI RGATCY 2 cut(s) 414, 880
BsuRI GGCC 3 cut(s) 793, 994, 1161
BtsCI GGATG 4 cut(s) 268, 730, 1489, 1539
BtsIMutI CAGTG 2 cut(s) 431, 1277
BveI ACCTGC 1 cut(s) 1403
Cac8I GCNNGC 3 cut(s) 356, 391, 852
CciI TCATGA 1 cut(s) 912
CfoI GCGC 1 cut(s) 972
Cfr10I RCCGGY 2 cut(s) 450, 1550
Cfr13I GGNCC 5 cut(s) 453, 993, 1159, 1160, 1415
CseI GACGC 1 cut(s) 704
Csp6I GTAC 4 cut(s) 409, 577, 687, 1627
CspAI ACCGGT 2 cut(s) 450, 1550
CviAII CATG 7 cut(s) 104, 359, 901, 913, 1217, 1394, 1476
CviQI GTAC 4 cut(s) 409, 577, 687, 1627
DdeI CTNAG 4 cut(s) 434, 1006, 1263, 1421
DpnI GATC 2 cut(s) 416, 882
DpnII GATC 2 cut(s) 414, 880
DraIII CACNNNGTG 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 682
Eco24I GRGCYC 2 cut(s) 209, 1163
Eco47I GGWCC 2 cut(s) 453, 1415
Eco53kI GAGCTC 1 cut(s) 207
Eco57I CTGAAG 2 cut(s) 858, 1565
EcoICRI GAGCTC 1 cut(s) 207
EcoO109I RGGNCCY 2 cut(s) 1159, 1415
EcoRII CCWGG 2 cut(s) 473, 995
EcoT14I CCWWGG 1 cut(s) 682
EcoT38I GRGCYC 2 cut(s) 209, 1163
ErhI CCWWGG 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 10
FaeI CATG 7 cut(s) 107, 362, 904, 916, 1220, 1397, 1479
FaqI GGGAC 5 cut(s) 439, 1264, 1316, 1401, 1404
FatI CATG 7 cut(s) 103, 358, 900, 912, 1216, 1393, 1475
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 1258
FokI GGATG 4 cut(s) 275, 717, 1496, 1526
FriOI GRGCYC 2 cut(s) 209, 1163
FspBI CTAG 1 cut(s) 1298
FspI TGCGCA 1 cut(s) 971
GlaI GCGC 1 cut(s) 971
GsaI CCCAGC 1 cut(s) 1166
GsuI CTGGAG 1 cut(s) 670
HaeIII GGCC 3 cut(s) 793, 994, 1161
HapII CCGG 2 cut(s) 451, 1551
HgaI GACGC 1 cut(s) 704
HhaI GCGC 1 cut(s) 972
Hin1II CATG 7 cut(s) 107, 362, 904, 916, 1220, 1397, 1479
Hin6I GCGC 1 cut(s) 970
HinP1I GCGC 1 cut(s) 970
HincII GTYRAC 1 cut(s) 1548
HindII GTYRAC 1 cut(s) 1548
HinfI GANTC 7 cut(s) 66, 82, 110, 149, 157, 163, 331
HpaI GTTAAC 1 cut(s) 1548
HpaII CCGG 2 cut(s) 451, 1551
HphI GGTGA 1 cut(s) 937
Hpy166II GTNNAC 3 cut(s) 571, 1189, 1548
Hpy188III TCNNGA 6 cut(s) 146, 397, 718, 734, 884, 913
Hpy8I GTNNAC 3 cut(s) 571, 1189, 1548
HpyAV CCTTC 6 cut(s) 6, 239, 576, 739, 843, 1358
HpyCH4III ACNGT 3 cut(s) 348, 1295, 1307
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 704, 938, 941, 1208, 1217, 1280, 1283
HpyF3I CTNAG 4 cut(s) 434, 1006, 1263, 1421
Hsp92II CATG 7 cut(s) 107, 362, 904, 916, 1220, 1397, 1479
HspAI GCGC 1 cut(s) 970
KspAI GTTAAC 1 cut(s) 1548
Kzo9I GATC 2 cut(s) 414, 880
LmnI GCTCC 1 cut(s) 168
LweI GCATC 4 cut(s) 553, 963, 1211, 1548
MaeI CTAG 1 cut(s) 1298
MaeIII GTNAC 1 cut(s) 1427
MalI GATC 2 cut(s) 416, 882
MboI GATC 2 cut(s) 414, 880
MboII GAAGA 6 cut(s) 236, 326, 637, 733, 851, 1571
MfeI CAATTG 1 cut(s) 859
MflI RGATCY 2 cut(s) 414, 880
MhlI GDGCHC 3 cut(s) 209, 1163, 1237
MlyI GAGTC 3 cut(s) 143, 151, 157
MmeI TCCRAC 4 cut(s) 140, 185, 537, 1334
MseI TTAA 9 cut(s) 92, 419, 446, 558, 1268, 1334, 1370, 1518, 1547
MslI CAYNNNNRTG 3 cut(s) 905, 1171, 1242
MspCI CTTAAG 3 cut(s) 91, 418, 1333
MspI CCGG 2 cut(s) 451, 1551
MspR9I CCNGG 2 cut(s) 475, 997
MunI CAATTG 1 cut(s) 859
Mva1269I GAATGC 1 cut(s) 912
MvaI CCWGG 2 cut(s) 475, 997
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 8 cut(s) 59, 704, 938, 941, 1208, 1217, 1280, 1283
NdeI CATATG 1 cut(s) 1258
NdeII GATC 2 cut(s) 414, 880
NlaIII CATG 7 cut(s) 107, 362, 904, 916, 1220, 1397, 1479
NlaIV GGNNCC 7 cut(s) 455, 680, 882, 1160, 1161, 1417, 1561
NmeAIII GCCGAG 1 cut(s) 769
NmuCI GTSAC 1 cut(s) 1427
NsbI TGCGCA 1 cut(s) 971
NspI RCATGY 3 cut(s) 904, 1220, 1397
NspV TTCGAA 1 cut(s) 1319
OliI CACNNNNGTG 1 cut(s) 1242
PagI TCATGA 1 cut(s) 912
PasI CCCWGGG 1 cut(s) 996
PciI ACATGT 1 cut(s) 1393
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 1325
PctI GAATGC 1 cut(s) 912
PfeI GAWTC 4 cut(s) 66, 82, 110, 331
PinAI ACCGGT 2 cut(s) 450, 1550
PleI GAGTC 3 cut(s) 143, 151, 157
PpsI GAGTC 3 cut(s) 143, 151, 157
PpuMI RGGWCCY 1 cut(s) 1415
PscI ACATGT 1 cut(s) 1393
PshAI GACNNNNGTC 1 cut(s) 632
PshBI ATTAAT 1 cut(s) 1518
PsiI TTATAA 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 1415
Psp6I CCWGG 2 cut(s) 473, 995
PspFI CCCAGC 1 cut(s) 1162
PspGI CCWGG 2 cut(s) 473, 995
PspN4I GGNNCC 7 cut(s) 455, 680, 882, 1160, 1161, 1417, 1561
PspOMI GGGCCC 1 cut(s) 1159
PspPI GGNCC 5 cut(s) 453, 993, 1159, 1160, 1415
PspPPI RGGWCCY 1 cut(s) 1415
PstI CTGCAG 1 cut(s) 1288
PsuI RGATCY 2 cut(s) 414, 880
RsaI GTAC 4 cut(s) 410, 578, 688, 1628
RsaNI GTAC 4 cut(s) 409, 577, 687, 1627
RseI CAYNNNNRTG 3 cut(s) 905, 1171, 1242
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 9 cut(s) 92, 419, 446, 558, 1268, 1334, 1370, 1518, 1547
Sau3AI GATC 2 cut(s) 414, 880
Sau96I GGNCC 5 cut(s) 453, 993, 1159, 1160, 1415
SchI GAGTC 3 cut(s) 143, 151, 157
ScrFI CCNGG 2 cut(s) 475, 997
SduI GDGCHC 3 cut(s) 209, 1163, 1237
SfaNI GCATC 4 cut(s) 553, 963, 1211, 1548
SfcI CTRYAG 2 cut(s) 27, 1284
SfuI TTCGAA 1 cut(s) 1319
SinI GGWCC 2 cut(s) 453, 1415
SmiMI CAYNNNNRTG 3 cut(s) 905, 1171, 1242
SmlI CTYRAG 4 cut(s) 91, 418, 988, 1333
SmoI CTYRAG 4 cut(s) 91, 418, 988, 1333
SsiI CCGC 4 cut(s) 62, 124, 328, 1116
SspMI CTAG 1 cut(s) 1298
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 473, 995
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 3 cut(s) 348, 1295, 1307
TaqI TCGA 2 cut(s) 203, 1319
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 126
TfiI GAWTC 4 cut(s) 66, 82, 110, 331
Tru1I TTAA 9 cut(s) 92, 419, 446, 558, 1268, 1334, 1370, 1518, 1547
Tru9I TTAA 9 cut(s) 92, 419, 446, 558, 1268, 1334, 1370, 1518, 1547
TscAI CASTG 2 cut(s) 438, 1284
TseFI GTSAC 1 cut(s) 1427
Tsp45I GTSAC 1 cut(s) 1427
TspDTI ATGAA 7 cut(s) 279, 417, 545, 638, 929, 1064, 1584
TspGWI ACGGA 2 cut(s) 594, 1614
TspRI CASTG 2 cut(s) 438, 1284
Vha464I CTTAAG 3 cut(s) 91, 418, 1333
VpaK11BI GGWCC 2 cut(s) 453, 1415
VspI ATTAAT 1 cut(s) 1518
XapI RAATTY 4 cut(s) 141, 482, 1139, 1321
XceI RCATGY 3 cut(s) 904, 1220, 1397
XcmI CCANNNNNNNNNTGG 1 cut(s) 1170
XspI CTAG 1 cut(s) 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.