Rh3DG053000

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000862
Physical Location & Seq
Forward (+)
3572078 .. 3629430
57353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG053000.1

Sequence Viewer

Length: 1560 bp
AGTGATCCCATTGTTGTGGTTTATGTGAAGAAAGGAGATGGTAAGCTAGAGGAACTAGGGAGAACTGAAGTAATACTGAATAGTTTGAATCCAGCGTGGATTGAGAAAATTACAGTTTCATATCAGTTTGAGGTTGTGCAGCCATTGATATTCCATGTCTATGATGTCGATACAAAATATCACAATGTAAATGTAAAGACACTGATGTTGGAGGATCAAGAATTTCTTGGGGAAGGCAATTGCGTTCTTTCAGAGATAGTGACCAAACAAAGTCGAAGTTTAACCTTGAATCTCCATAACAGAAATGGGCGTGGAGGTTTGAGAAACTTTGGAACACTCACTGTCCATGCTGAAGAAACTATGGCTTCAAAGAGTGTTGTTGAGATAAAATTCCATTGTTCTGAATTGGAAAACAAAGACCTCTTCTCTAAAAGTGATCCTTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTGGTAGATAACAATTTAAATCCAATCTGGAAACCACTATCCCTGAGTATGCAGCAGTTTGGAAGCAAGGATAATCCATTAGTCATCGAGTGCTTTGATTTCAACAGCAATGGCAGTCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCCGAGCTGGAGAGATTATACAAAGAAAGAAGTGGGGTGAATTTTGTCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTAGTGGATCAATTTAATGAGAAGCAACAGTTCAGCTTTCTCGATTATATTTCCAGTGGATTTGAGCTTAACTTCATGGTTGCGGTTGACTTTACGGCTTCAAATGGAAACCCTCAACAACATGATTCATTGCACTACAATGATCCATATGGCCGGTTGAATTCTTACCAGCAGGCAATTACTGAAGTTGGGGAAGTCATTCAGTTTTATGATGCTGATAAACGGTTTCCTGCTTGGGGCTTTGGAGGAAGGACAGCTGGAGGACCTGTCTCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTTGAGGGAGTTCAAGGCATAATGACAGCTTATGCCAGTGCCCTACACAATGTTTCTCTATCAGGACCCACTTTGTTTGGCCCAGTGGTTACTAGGGCTGCGGAGATTGCTGGCAATTCTCTATTCCACAACAACAATAAGTACTATGTGCTGCTGATCATAACGGATGGGATACTTGCAGACCTGCAGGAAACAACAGATGCTTTGGTGAGGGCATCTGATCTTCCCCTCTCAATTCTAATAGTTGGAGTAGGAAATGCAGATTTCAAACAAATGGAGATTCTCGATGCTGACAATGGACCGCGATTATCGAGTTCAACAGGTCGTGTGGCTACCAGAGACATTGTACAATTTGTTCCCATGAGAGAAGTGCAGAGTGGGCGCATTTCTGCAGTTCAATCGCTTCTCGAAGAGCTGCCTGGACAGTTCTTGAGTTACATGCGCTGCAGAGACATCATGCCTCTCACTGTCTGTGCAGCCCAATCATCAGCTCCACAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

519

Amino Acids

57.33

Weight (kDa)

5.32

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 1 - 93 4.5e-09 C2 domain
C2 PF00168 132 - 221 2.3e-12 C2 domain
Copine PF07002 291 - 505 5e-76 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1248
AccII CGCG 1 cut(s) 1360
AciI CCGC 3 cut(s) 827, 1157, 1358
AclWI GGATC 4 cut(s) 222, 431, 759, 881
AcoI YGGCCR 2 cut(s) 651, 895
AcsI RAATTY 4 cut(s) 221, 389, 691, 904
AcuI CTGAAG 4 cut(s) 87, 372, 519, 948
AfaI GTAC 2 cut(s) 1199, 1404
AfiI CCNNNNNNNGG 1 cut(s) 980
AflII CTTAAG 1 cut(s) 445
AjnI CCWGG 1 cut(s) 1474
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 8 cut(s) 46, 658, 780, 811, 1001, 1085, 1471, 1547
AluI AGCT 8 cut(s) 46, 658, 780, 811, 1001, 1085, 1471, 1547
Alw26I GTCTC 3 cut(s) 1018, 1389, 1500
AlwI GGATC 4 cut(s) 222, 431, 759, 881
AoxI GGCC 3 cut(s) 651, 895, 1135
ApeKI GCWGC 7 cut(s) 139, 553, 1154, 1207, 1471, 1500, 1532
ApoI RAATTY 4 cut(s) 221, 389, 691, 904
Asp700I GAANNNNTTC 1 cut(s) 942
AspLEI GCGC 2 cut(s) 1440, 1500
AspS9I GGNCC 4 cut(s) 1007, 1121, 1136, 1355
AsuHPI GGTGA 2 cut(s) 700, 1276
AvaII GGWCC 3 cut(s) 1007, 1121, 1355
BaeGI GKGCMC 1 cut(s) 1099
BbvI GCAGC 7 cut(s) 151, 565, 1141, 1194, 1458, 1487, 1544
BccI CCATC 4 cut(s) 32, 712, 1028, 1217
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 1437, 1471
BciT130I CCWGG 1 cut(s) 1476
BciVI GTATCC 1 cut(s) 1221
BclI TGATCA 1 cut(s) 1212
BcoDI GTCTC 3 cut(s) 1018, 1389, 1500
BfaI CTAG 4 cut(s) 47, 56, 456, 1149
BfmI CTRYAG 3 cut(s) 1241, 1446, 1501
BfrI CTTAAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 1248
BfuI GTATCC 1 cut(s) 1221
BisI GCNGC 7 cut(s) 140, 554, 1155, 1208, 1472, 1501, 1533
BlsI GCNGC 7 cut(s) 141, 555, 1156, 1209, 1473, 1502, 1534
BmcAI AGTACT 1 cut(s) 1199
Bme1390I CCNGG 1 cut(s) 1476
Bme18I GGWCC 3 cut(s) 1007, 1121, 1355
BmgT120I GGNCC 4 cut(s) 1007, 1121, 1136, 1355
BmiI GGNNCC 1 cut(s) 1123
BmrFI CCNGG 1 cut(s) 1476
BmrI ACTGGG 1 cut(s) 1133
BmsI GCATC 4 cut(s) 946, 1246, 1280, 1333
BmuI ACTGGG 1 cut(s) 1133
BpmI CTGGAG 2 cut(s) 680, 1023
BpuEI CTTGAG 1 cut(s) 1507
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 996, 1026
Bsc4I CCNNNNNNNGG 1 cut(s) 980
Bse118I RCCGGY 1 cut(s) 897
Bse1I ACTGG 4 cut(s) 798, 1051, 1092, 1139
Bse3DI GCAATG 2 cut(s) 616, 872
BseBI CCWGG 1 cut(s) 1476
BseGI GGATG 2 cut(s) 1039, 1228
BseLI CCNNNNNNNGG 1 cut(s) 980
BseMI GCAATG 2 cut(s) 616, 872
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 4 cut(s) 798, 1051, 1092, 1139
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 7 cut(s) 151, 565, 1141, 1194, 1458, 1487, 1544
BsgI GTGCAG 3 cut(s) 158, 1448, 1551
Bsh1236I CGCG 1 cut(s) 1360
BshFI GGCC 3 cut(s) 653, 897, 1137
BsiSI CCGG 1 cut(s) 898
BslI CCNNNNNNNGG 1 cut(s) 980
BsmAI GTCTC 3 cut(s) 1018, 1389, 1500
BsnI GGCC 3 cut(s) 653, 897, 1137
Bsp1286I GDGCHC 1 cut(s) 1099
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
BspACI CCGC 3 cut(s) 827, 1157, 1358
BspANI GGCC 3 cut(s) 653, 897, 1137
BspCNI CTCAG 1 cut(s) 537
BspFNI CGCG 1 cut(s) 1360
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 1 cut(s) 1123
BspMAI CTGCAG 3 cut(s) 1245, 1450, 1505
BspMI ACCTGC 1 cut(s) 1248
BspPI GGATC 4 cut(s) 222, 431, 759, 881
BspQI GCTCTTC 1 cut(s) 1461
BspTI CTTAAG 1 cut(s) 445
BsrDI GCAATG 2 cut(s) 616, 872
BsrFI RCCGGY 1 cut(s) 897
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 4 cut(s) 798, 1051, 1092, 1139
BssAI RCCGGY 1 cut(s) 897
BssMI GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
Bst2UI CCWGG 1 cut(s) 1476
Bst4CI ACNGT 7 cut(s) 115, 343, 774, 969, 1022, 1482, 1525
Bst6I CTCTTC 2 cut(s) 428, 1461
BstAFI CTTAAG 1 cut(s) 445
BstAUI TGTACA 1 cut(s) 1402
BstC8I GCNNGC 2 cut(s) 918, 1168
BstDEI CTNAG 1 cut(s) 545
BstF5I GGATG 2 cut(s) 1039, 1228
BstFNI CGCG 1 cut(s) 1360
BstHHI GCGC 2 cut(s) 1440, 1500
BstKTI GATC 7 cut(s) 7, 217, 439, 754, 889, 1215, 1279
BstMAI GTCTC 3 cut(s) 1018, 1389, 1500
BstMBI GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
BstMWI GCNNNNNNNGC 2 cut(s) 1163, 1435
BstNI CCWGG 1 cut(s) 1476
BstNSI RCATGY 1 cut(s) 1498
BstSCI CCNGG 1 cut(s) 1474
BstSFI CTRYAG 3 cut(s) 1241, 1446, 1501
BstSLI GKGCMC 1 cut(s) 1099
BstUI CGCG 1 cut(s) 1360
BstV1I GCAGC 7 cut(s) 151, 565, 1141, 1194, 1458, 1487, 1544
BstXI CCANNNNNNTGG 1 cut(s) 16
BsuI GTATCC 1 cut(s) 1221
BsuRI GGCC 3 cut(s) 653, 897, 1137
BtsCI GGATG 2 cut(s) 1039, 1228
BtsIMutI CAGTG 8 cut(s) 200, 339, 654, 805, 1018, 1099, 1146, 1521
BveI ACCTGC 1 cut(s) 1248
Cac8I GCNNGC 2 cut(s) 918, 1168
CciI TCATGA 1 cut(s) 718
CfoI GCGC 2 cut(s) 1440, 1500
Cfr10I RCCGGY 1 cut(s) 897
Cfr13I GGNCC 4 cut(s) 1007, 1121, 1136, 1355
Csp6I GTAC 2 cut(s) 1198, 1403
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviQI GTAC 2 cut(s) 1198, 1403
DdeI CTNAG 1 cut(s) 545
DpnI GATC 7 cut(s) 6, 216, 438, 753, 888, 1214, 1278
DpnII GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 2 cut(s) 651, 895
Eam1104I CTCTTC 2 cut(s) 428, 1461
EarI CTCTTC 2 cut(s) 428, 1461
Eco47I GGWCC 3 cut(s) 1007, 1121, 1355
Eco57I CTGAAG 4 cut(s) 87, 372, 519, 948
EcoO109I RGGNCCY 2 cut(s) 1007, 1121
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 1 cut(s) 1474
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 424, 456
FauNDI CATATG 1 cut(s) 892
FbaI TGATCA 1 cut(s) 1212
Fnu4HI GCNGC 7 cut(s) 140, 554, 1155, 1208, 1472, 1501, 1533
FokI GGATG 2 cut(s) 1046, 1235
Fsp4HI GCNGC 7 cut(s) 140, 554, 1155, 1208, 1472, 1501, 1533
FspBI CTAG 4 cut(s) 47, 56, 456, 1149
GlaI GCGC 2 cut(s) 1439, 1499
GluI GCNGC 7 cut(s) 140, 554, 1155, 1208, 1472, 1501, 1533
GsuI CTGGAG 2 cut(s) 680, 1023
HaeIII GGCC 3 cut(s) 653, 897, 1137
HapII CCGG 1 cut(s) 898
HhaI GCGC 2 cut(s) 1440, 1500
Hin6I GCGC 2 cut(s) 1438, 1498
HinP1I GCGC 2 cut(s) 1438, 1498
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 4 cut(s) 88, 289, 869, 1336
HpaII CCGG 1 cut(s) 898
HphI GGTGA 2 cut(s) 700, 1276
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 3 cut(s) 253, 403, 1276
Hpy188III TCNNGA 9 cut(s) 218, 456, 529, 719, 785, 1119, 1340, 1463, 1486
Hpy8I GTNNAC 1 cut(s) 832
HpyAV CCTTC 2 cut(s) 227, 987
HpyCH4III ACNGT 7 cut(s) 115, 343, 774, 969, 1022, 1482, 1525
HpyF10VI GCNNNNNNNGC 2 cut(s) 1163, 1435
HpyF3I CTNAG 1 cut(s) 545
HspAI GCGC 2 cut(s) 1438, 1498
Ksp22I TGATCA 1 cut(s) 1212
Kzo9I GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
LguI GCTCTTC 1 cut(s) 1461
LmnI GCTCC 1 cut(s) 1552
Lsp1109I GCAGC 7 cut(s) 151, 565, 1141, 1194, 1458, 1487, 1544
LweI GCATC 4 cut(s) 946, 1246, 1280, 1333
MaeI CTAG 4 cut(s) 47, 56, 456, 1149
MaeIII GTNAC 3 cut(s) 259, 1144, 1490
MalI GATC 7 cut(s) 6, 216, 438, 753, 888, 1214, 1278
MboI GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
MboII GAAGA 6 cut(s) 40, 365, 415, 699, 1271, 1478
MfeI CAATTG 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 1099
MmeI TCCRAC 2 cut(s) 189, 1282
MroXI GAANNNNTTC 1 cut(s) 942
MseI TTAA 6 cut(s) 281, 446, 518, 759, 813, 1026
MslI CAYNNNNRTG 4 cut(s) 14, 159, 717, 882
MspA1I CMGCKG 1 cut(s) 1001
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 1 cut(s) 898
MspR9I CCNGG 1 cut(s) 1476
MunI CAATTG 1 cut(s) 238
MvaI CCWGG 1 cut(s) 1476
MvnI CGCG 1 cut(s) 1360
MwoI GCNNNNNNNGC 2 cut(s) 1163, 1435
NdeI CATATG 1 cut(s) 892
NdeII GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
NlaIV GGNNCC 1 cut(s) 1123
NmeAIII GCCGAG 1 cut(s) 679
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 1498
PagI TCATGA 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 1461
PdmI GAANNNNTTC 1 cut(s) 942
PfeI GAWTC 4 cut(s) 88, 289, 869, 1336
PkrI GCNGC 7 cut(s) 141, 555, 1156, 1209, 1473, 1502, 1534
PpuMI RGGWCCY 2 cut(s) 1007, 1121
Psp5II RGGWCCY 2 cut(s) 1007, 1121
Psp6I CCWGG 1 cut(s) 1474
PspGI CCWGG 1 cut(s) 1474
PspN4I GGNNCC 1 cut(s) 1123
PspPI GGNCC 4 cut(s) 1007, 1121, 1136, 1355
PspPPI RGGWCCY 2 cut(s) 1007, 1121
PsrI GAACNNNNNNTAC 2 cut(s) 1395, 1427
PstI CTGCAG 3 cut(s) 1245, 1450, 1505
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 2 cut(s) 1199, 1404
RsaNI GTAC 2 cut(s) 1198, 1403
RseI CAYNNNNRTG 4 cut(s) 14, 159, 717, 882
SapI GCTCTTC 1 cut(s) 1461
SaqAI TTAA 6 cut(s) 281, 446, 518, 759, 813, 1026
SatI GCNGC 7 cut(s) 140, 554, 1155, 1208, 1472, 1501, 1533
Sau3AI GATC 7 cut(s) 4, 214, 436, 751, 886, 1212, 1276
Sau96I GGNCC 4 cut(s) 1007, 1121, 1136, 1355
SbfI CCTGCAGG 1 cut(s) 1245
ScaI AGTACT 1 cut(s) 1199
ScrFI CCNGG 1 cut(s) 1476
SdaI CCTGCAGG 1 cut(s) 1245
SduI GDGCHC 1 cut(s) 1099
SfaNI GCATC 4 cut(s) 946, 1246, 1280, 1333
SfcI CTRYAG 3 cut(s) 1241, 1446, 1501
SinI GGWCC 3 cut(s) 1007, 1121, 1355
SmiI ATTTAAAT 1 cut(s) 519
SmiMI CAYNNNNRTG 4 cut(s) 14, 159, 717, 882
SmlI CTYRAG 2 cut(s) 445, 1486
SmoI CTYRAG 2 cut(s) 445, 1486
Sse8387I CCTGCAGG 1 cut(s) 1245
SsiI CCGC 3 cut(s) 827, 1157, 1358
SspMI CTAG 4 cut(s) 47, 56, 456, 1149
StyD4I CCNGG 1 cut(s) 1474
SwaI ATTTAAAT 1 cut(s) 519
TaaI ACNGT 7 cut(s) 115, 343, 774, 969, 1022, 1482, 1525
TaqI TCGA 7 cut(s) 168, 274, 588, 786, 1341, 1367, 1464
TatI WGTACW 2 cut(s) 1197, 1402
TfiI GAWTC 4 cut(s) 88, 289, 869, 1336
Tru1I TTAA 6 cut(s) 281, 446, 518, 759, 813, 1026
Tru9I TTAA 6 cut(s) 281, 446, 518, 759, 813, 1026
TscAI CASTG 8 cut(s) 207, 346, 654, 805, 1025, 1099, 1146, 1528
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 7 cut(s) 139, 553, 1154, 1207, 1471, 1500, 1532
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 4 cut(s) 108, 735, 808, 861
TspGWI ACGGA 1 cut(s) 1235
TspRI CASTG 8 cut(s) 207, 346, 654, 805, 1025, 1099, 1146, 1528
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 3 cut(s) 1007, 1121, 1355
XapI RAATTY 4 cut(s) 221, 389, 691, 904
XbaI TCTAGA 1 cut(s) 455
XceI RCATGY 1 cut(s) 1498
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 942
XspI CTAG 4 cut(s) 47, 56, 456, 1149
ZrmI AGTACT 1 cut(s) 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.