RchiOBHm_Chr3g0453381

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000862
Physical Location & Seq
Forward (+)
3808228 .. 3812143
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42046

Sequence Viewer

Length: 1701 bp
ATGGTTCTCTTATTTGTAGTTCTATTCATTTTCATATATTTTTCTAGTTGGACCTTCTATTTCCTTCTTTGGAATGTAATGTCGGATTTCTTATCTGTGTTACAGTTATCGCTGTCAGCAACTAACTTGCTTGATCGTGACATTATGTCAAAGAGTGATCCTATGGTTGTGGTTTATGTGAAGAAAGGAGATGGTAAGCTAGAGGAACTAGGAAGAACTGAAGTAATACTGAATAGTTTGAAACCAGTGTGGATTGAGAAAATCACAGTCTCATATCAGTTCGAGATTGTGCAGTCATTGATATTTCATGTCTATGATGTCGATACAAAATATCACAACATAAATGTAAAGACACTGAAGTTGGAGGATCAAGAATTTCTTGGGGAAGGCAGTTGCGTTCTTTCAGAGATAGTGACCAAACAAAGTCGAAGTTTAACCTTAAATCTGCATAACAGAAGTGGGCGTGGCTTAGGAACACTCACAGTCCATGCTGAAGAAACTATGGCTTCGAAGAGTGTTGTTGAGATAAAATTCCGTTGTTCCCAATTAGAAAACAAAGACCTCTTCTCTAAAAGTGATCCTTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTAGTGGATAACAATTTAAATCCAATTTGGAAACCACTATCCCTCAGTATGCAGCAGTTTGGAAGCAAGGATAATCCATTAGTCATCGAATGCTTTGATTTCAACAGCAATGGCAATCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCAGACCTGGAGAGATTATACAAAGAAAGAAGTGGGGTGAATTTCGTCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTTGTGGATCAATTTAATGAGAAGCAACAGTTCAGCTTTCTCGATTATATATCCAGTGGATTTGAACTTAACTTCATGGTTGCCGTTGACTTTACGGCTTCAAATGGAAATCCTCAACAACATGATTCGTTGCACTACATTGATCCGTATGGCAGATTAAATTCTTACCAGCAGGCAGTAACAGAAGTTGGGGAAGTCATTCAGTTTTATGATACTGACAAACGCTTTCCTGCTTGGGGCTTTGGAGGAAGGACAGCTGGAGGACCTGTATCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTTGAAGGGGTTCAAGGCATAATGACTGCGTATGCCGGTGCCCTACACAATGTTTCTCTATCAGGACCCACTTTGTTTGGCCCAGTGGTTAATAGGGCTGCAGAGATTGCTGGCAATTCTCTATTCCACAACAACAATAAGTACTATGTGCTACTGATCATTACGGATGGGATACTTACAGACCTGCAGGAAACAACAGATGCTTTGGTGAGGGCATCTGATCTTCCCCTCTCAGTTCTTATAGTTGGAGTGGGAAGTGCGGAATTCAAACAAATGGAGATTCTCGATGCTGACAATGGACAGCGATTATCAAGTTCAACAGGCCGTGTGGCTACCAGAGACATTGTACAATTTGTTCCTATGAGAGAAGTGCAGAGTGGGCGCATTTCTGCTGTTCAATCGCTTCTCGAAGAGCTGCCTGGACAGTTCTTGAGTTACATGCGCTGCAGAGACGTCATGCCTCTCACTGTCCATGCAGCCCAATCATCTGCTCCACAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

566

Amino Acids

63.06

Weight (kDa)

5.46

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 38 - 143 1.4e-12 C2 domain
C2 PF00168 179 - 268 1.3e-12 C2 domain
Copine PF07002 338 - 551 1.9e-75 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1653
Acc36I ACCTGC 1 cut(s) 1389
AccB1I GGYRCC 1 cut(s) 1235
AciI CCGC 1 cut(s) 1457
AclWI GGATC 5 cut(s) 152, 375, 572, 900, 1022
AcsI RAATTY 5 cut(s) 374, 530, 832, 1045, 1460
AcuI CTGAAG 4 cut(s) 240, 377, 513, 660
AcyI GRCGYC 1 cut(s) 1650
AfaI GTAC 2 cut(s) 1340, 1545
AfiI CCNNNNNNNGG 1 cut(s) 1121
AflII CTTAAG 1 cut(s) 586
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 798, 1615
AloI GAACNNNNNNTCC 2 cut(s) 606, 638
AluBI AGCT 4 cut(s) 199, 921, 1142, 1612
AluI AGCT 4 cut(s) 199, 921, 1142, 1612
Alw26I GTCTC 3 cut(s) 274, 1530, 1641
AlwI GGATC 5 cut(s) 152, 375, 572, 900, 1022
AoxI GGCC 2 cut(s) 1276, 1519
ApeKI GCWGC 5 cut(s) 694, 1295, 1612, 1641, 1673
ApoI RAATTY 5 cut(s) 374, 530, 832, 1045, 1460
Asp700I GAANNNNTTC 2 cut(s) 1083, 1206
AspLEI GCGC 2 cut(s) 1581, 1641
AspS9I GGNCC 4 cut(s) 51, 1148, 1262, 1277
AsuHPI GGTGA 2 cut(s) 841, 1417
AsuII TTCGAA 1 cut(s) 509
AvaII GGWCC 3 cut(s) 51, 1148, 1262
BaeGI GKGCMC 1 cut(s) 1240
BanI GGYRCC 1 cut(s) 1235
BbvI GCAGC 5 cut(s) 706, 1282, 1599, 1628, 1685
BccI CCATC 4 cut(s) 185, 853, 1169, 1358
BceAI ACGGC 3 cut(s) 953, 996, 1506
BciT130I CCWGG 2 cut(s) 800, 1617
BciVI GTATCC 1 cut(s) 1362
BclI TGATCA 1 cut(s) 1353
BcoDI GTCTC 3 cut(s) 274, 1530, 1641
BfaI CTAG 4 cut(s) 45, 200, 209, 597
BfmI CTRYAG 3 cut(s) 1296, 1382, 1642
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 1389
BfuI GTATCC 1 cut(s) 1362
BisI GCNGC 5 cut(s) 695, 1296, 1613, 1642, 1674
BlsI GCNGC 5 cut(s) 696, 1297, 1614, 1643, 1675
BmcAI AGTACT 1 cut(s) 1340
Bme1390I CCNGG 2 cut(s) 800, 1617
Bme18I GGWCC 3 cut(s) 51, 1148, 1262
BmeRI GACNNNNNGTC 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 51, 1148, 1262, 1277
BmiI GGNNCC 2 cut(s) 1237, 1264
BmrFI CCNGG 2 cut(s) 800, 1617
BmrI ACTGGG 1 cut(s) 1274
BmsI GCATC 3 cut(s) 1387, 1421, 1474
BmuI ACTGGG 1 cut(s) 1274
BpmI CTGGAG 2 cut(s) 821, 1164
Bpu10I CCTNAGC 1 cut(s) 469
Bpu14I TTCGAA 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 1648
BsaHI GRCGYC 1 cut(s) 1650
BsaXI ACNNNNNCTCC 4 cut(s) 606, 636, 1137, 1167
Bsc4I CCNNNNNNNGG 1 cut(s) 1121
Bse118I RCCGGY 1 cut(s) 1232
Bse1I ACTGG 4 cut(s) 245, 939, 1192, 1280
Bse3DI GCAATG 1 cut(s) 757
BseBI CCWGG 2 cut(s) 800, 1617
BseGI GGATG 2 cut(s) 1180, 1369
BseLI CCNNNNNNNGG 1 cut(s) 1121
BseMI GCAATG 1 cut(s) 757
BseMII CTCAG 2 cut(s) 700, 1443
BseNI ACTGG 4 cut(s) 245, 939, 1192, 1280
BseSI GKGCMC 1 cut(s) 1240
BseXI GCAGC 5 cut(s) 706, 1282, 1599, 1628, 1685
BsgI GTGCAG 2 cut(s) 311, 1589
BshFI GGCC 2 cut(s) 1278, 1521
BshNI GGYRCC 1 cut(s) 1235
BsiSI CCGG 1 cut(s) 1233
BslI CCNNNNNNNGG 1 cut(s) 1121
BsmAI GTCTC 3 cut(s) 274, 1530, 1641
BsmBI CGTCTC 1 cut(s) 1641
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 1278, 1521
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 1240
Bsp1407I TGTACA 1 cut(s) 1543
Bsp143I GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
BspACI CCGC 1 cut(s) 1457
BspANI GGCC 2 cut(s) 1278, 1521
BspCNI CTCAG 2 cut(s) 699, 1442
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 2 cut(s) 1237, 1264
BspMAI CTGCAG 3 cut(s) 1300, 1386, 1646
BspMI ACCTGC 1 cut(s) 1389
BspPI GGATC 5 cut(s) 152, 375, 572, 900, 1022
BspQI GCTCTTC 1 cut(s) 1602
BspT104I TTCGAA 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 1235
BspTI CTTAAG 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 757
BsrFI RCCGGY 1 cut(s) 1232
BsrGI TGTACA 1 cut(s) 1543
BsrI ACTGG 4 cut(s) 245, 939, 1192, 1280
BssAI RCCGGY 1 cut(s) 1232
BssMI GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
BssNI GRCGYC 1 cut(s) 1650
Bst2UI CCWGG 2 cut(s) 800, 1617
Bst4CI ACNGT 7 cut(s) 105, 268, 484, 915, 1163, 1623, 1666
Bst6I CTCTTC 3 cut(s) 506, 569, 1602
BstACI GRCGYC 1 cut(s) 1650
BstAFI CTTAAG 1 cut(s) 586
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstAUI TGTACA 1 cut(s) 1543
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 1059, 1309
BstDEI CTNAG 3 cut(s) 469, 686, 1429
BstF5I GGATG 2 cut(s) 1180, 1369
BstHHI GCGC 2 cut(s) 1581, 1641
BstKTI GATC 8 cut(s) 136, 160, 370, 580, 895, 1030, 1356, 1420
BstMAI GTCTC 3 cut(s) 274, 1530, 1641
BstMBI GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
BstMWI GCNNNNNNNGC 2 cut(s) 1304, 1576
BstNI CCWGG 2 cut(s) 800, 1617
BstNSI RCATGY 1 cut(s) 1639
BstSCI CCNGG 2 cut(s) 798, 1615
BstSFI CTRYAG 3 cut(s) 1296, 1382, 1642
BstSLI GKGCMC 1 cut(s) 1240
BstV1I GCAGC 5 cut(s) 706, 1282, 1599, 1628, 1685
BsuI GTATCC 1 cut(s) 1362
BsuRI GGCC 2 cut(s) 1278, 1521
BtsCI GGATG 2 cut(s) 1180, 1369
BtsIMutI CAGTG 7 cut(s) 252, 353, 795, 946, 1159, 1287, 1662
BveI ACCTGC 1 cut(s) 1389
Cac8I GCNNGC 2 cut(s) 1059, 1309
CciI TCATGA 1 cut(s) 859
CfoI GCGC 2 cut(s) 1581, 1641
Cfr10I RCCGGY 1 cut(s) 1232
Cfr13I GGNCC 4 cut(s) 51, 1148, 1262, 1277
Csp6I GTAC 2 cut(s) 1339, 1544
CspCI CAANNNNNGTGG 2 cut(s) 1255, 1290
CviQI GTAC 2 cut(s) 1339, 1544
DdeI CTNAG 3 cut(s) 469, 686, 1429
DpnI GATC 8 cut(s) 135, 159, 369, 579, 894, 1029, 1355, 1419
DpnII GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
DraI TTTAAA 1 cut(s) 660
DriI GACNNNNNGTC 1 cut(s) 145
Eam1104I CTCTTC 3 cut(s) 506, 569, 1602
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 3 cut(s) 506, 569, 1602
Eco47I GGWCC 3 cut(s) 51, 1148, 1262
Eco57I CTGAAG 4 cut(s) 240, 377, 513, 660
EcoO109I RGGNCCY 2 cut(s) 1148, 1262
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 2 cut(s) 798, 1615
Esp3I CGTCTC 1 cut(s) 1641
FalI AAGNNNNNCTT 4 cut(s) 363, 395, 565, 597
FbaI TGATCA 1 cut(s) 1353
Fnu4HI GCNGC 5 cut(s) 695, 1296, 1613, 1642, 1674
FokI GGATG 2 cut(s) 1187, 1376
Fsp4HI GCNGC 5 cut(s) 695, 1296, 1613, 1642, 1674
FspBI CTAG 4 cut(s) 45, 200, 209, 597
GlaI GCGC 2 cut(s) 1580, 1640
GluI GCNGC 5 cut(s) 695, 1296, 1613, 1642, 1674
GsuI CTGGAG 2 cut(s) 821, 1164
HaeIII GGCC 2 cut(s) 1278, 1521
HapII CCGG 1 cut(s) 1233
HhaI GCGC 2 cut(s) 1581, 1641
Hin1I GRCGYC 1 cut(s) 1650
Hin6I GCGC 2 cut(s) 1579, 1639
HinP1I GCGC 2 cut(s) 1579, 1639
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HinfI GANTC 2 cut(s) 1010, 1477
HpaII CCGG 1 cut(s) 1233
HphI GGTGA 2 cut(s) 841, 1417
Hpy166II GTNNAC 1 cut(s) 973
Hpy188I TCNGA 3 cut(s) 85, 406, 1417
Hpy8I GTNNAC 1 cut(s) 973
HpyAV CCTTC 5 cut(s) 64, 74, 380, 1128, 1196
HpyCH4III ACNGT 7 cut(s) 105, 268, 484, 915, 1163, 1623, 1666
HpyCH4IV ACGT 1 cut(s) 1650
HpyF10VI GCNNNNNNNGC 2 cut(s) 1304, 1576
HpyF3I CTNAG 3 cut(s) 469, 686, 1429
HpySE526I ACGT 1 cut(s) 1650
Hsp92I GRCGYC 1 cut(s) 1650
HspAI GCGC 2 cut(s) 1579, 1639
Ksp22I TGATCA 1 cut(s) 1353
Kzo9I GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
LguI GCTCTTC 1 cut(s) 1602
LmnI GCTCC 1 cut(s) 1693
Lsp1109I GCAGC 5 cut(s) 706, 1282, 1599, 1628, 1685
LweI GCATC 3 cut(s) 1387, 1421, 1474
MaeI CTAG 4 cut(s) 45, 200, 209, 597
MaeII ACGT 1 cut(s) 1650
MaeIII GTNAC 5 cut(s) 99, 137, 412, 1063, 1631
MalI GATC 8 cut(s) 135, 159, 369, 579, 894, 1029, 1355, 1419
MboI GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
MboII GAAGA 8 cut(s) 193, 225, 506, 523, 556, 840, 1412, 1619
MhlI GDGCHC 1 cut(s) 1240
MmeI TCCRAC 4 cut(s) 29, 63, 342, 1423
MroXI GAANNNNTTC 2 cut(s) 1083, 1206
MseI TTAA 9 cut(s) 434, 440, 587, 659, 900, 954, 1043, 1167, 1287
MslI CAYNNNNRTG 2 cut(s) 312, 858
MspA1I CMGCKG 1 cut(s) 1142
MspCI CTTAAG 1 cut(s) 586
MspI CCGG 1 cut(s) 1233
MspR9I CCNGG 2 cut(s) 800, 1617
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 2 cut(s) 800, 1617
MwoI GCNNNNNNNGC 2 cut(s) 1304, 1576
NdeII GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
NlaIV GGNNCC 2 cut(s) 1237, 1264
NmuCI GTSAC 2 cut(s) 137, 412
NspI RCATGY 1 cut(s) 1639
NspV TTCGAA 1 cut(s) 509
PagI TCATGA 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 1602
PctI GAATGC 1 cut(s) 737
PdmI GAANNNNTTC 2 cut(s) 1083, 1206
PfeI GAWTC 2 cut(s) 1010, 1477
PkrI GCNGC 5 cut(s) 696, 1297, 1614, 1643, 1675
PpuMI RGGWCCY 2 cut(s) 1148, 1262
Psp5II RGGWCCY 2 cut(s) 1148, 1262
Psp6I CCWGG 2 cut(s) 798, 1615
PspGI CCWGG 2 cut(s) 798, 1615
PspN4I GGNNCC 2 cut(s) 1237, 1264
PspPI GGNCC 4 cut(s) 51, 1148, 1262, 1277
PspPPI RGGWCCY 2 cut(s) 1148, 1262
PsrI GAACNNNNNNTAC 2 cut(s) 1536, 1568
PstI CTGCAG 3 cut(s) 1300, 1386, 1646
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 2 cut(s) 1340, 1545
RsaNI GTAC 2 cut(s) 1339, 1544
RseI CAYNNNNRTG 2 cut(s) 312, 858
SapI GCTCTTC 1 cut(s) 1602
SaqAI TTAA 9 cut(s) 434, 440, 587, 659, 900, 954, 1043, 1167, 1287
SatI GCNGC 5 cut(s) 695, 1296, 1613, 1642, 1674
Sau3AI GATC 8 cut(s) 133, 157, 367, 577, 892, 1027, 1353, 1417
Sau96I GGNCC 4 cut(s) 51, 1148, 1262, 1277
SbfI CCTGCAGG 1 cut(s) 1386
ScaI AGTACT 1 cut(s) 1340
ScrFI CCNGG 2 cut(s) 800, 1617
SdaI CCTGCAGG 1 cut(s) 1386
SduI GDGCHC 1 cut(s) 1240
SfaNI GCATC 3 cut(s) 1387, 1421, 1474
SfcI CTRYAG 3 cut(s) 1296, 1382, 1642
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 3 cut(s) 51, 1148, 1262
SmiI ATTTAAAT 1 cut(s) 660
SmiMI CAYNNNNRTG 2 cut(s) 312, 858
SmlI CTYRAG 2 cut(s) 586, 1627
SmoI CTYRAG 2 cut(s) 586, 1627
Sse8387I CCTGCAGG 1 cut(s) 1386
SsiI CCGC 1 cut(s) 1457
SspMI CTAG 4 cut(s) 45, 200, 209, 597
StyD4I CCNGG 2 cut(s) 798, 1615
SwaI ATTTAAAT 1 cut(s) 660
TaaI ACNGT 7 cut(s) 105, 268, 484, 915, 1163, 1623, 1666
TaiI ACGT 1 cut(s) 1653
TaqI TCGA 8 cut(s) 282, 321, 427, 509, 729, 927, 1482, 1605
TatI WGTACW 2 cut(s) 1338, 1543
TfiI GAWTC 2 cut(s) 1010, 1477
Tru1I TTAA 9 cut(s) 434, 440, 587, 659, 900, 954, 1043, 1167, 1287
Tru9I TTAA 9 cut(s) 434, 440, 587, 659, 900, 954, 1043, 1167, 1287
TscAI CASTG 7 cut(s) 252, 360, 795, 946, 1166, 1287, 1669
TseFI GTSAC 2 cut(s) 137, 412
TseI GCWGC 5 cut(s) 694, 1295, 1612, 1641, 1673
Tsp45I GTSAC 2 cut(s) 137, 412
TspDTI ATGAA 5 cut(s) 16, 22, 296, 876, 949
TspGWI ACGGA 3 cut(s) 524, 1020, 1376
TspRI CASTG 7 cut(s) 252, 360, 795, 946, 1166, 1287, 1669
Vha464I CTTAAG 1 cut(s) 586
VpaK11BI GGWCC 3 cut(s) 51, 1148, 1262
XapI RAATTY 5 cut(s) 374, 530, 832, 1045, 1460
XbaI TCTAGA 1 cut(s) 596
XceI RCATGY 1 cut(s) 1639
XmnI GAANNNNTTC 2 cut(s) 1083, 1206
XspI CTAG 4 cut(s) 45, 200, 209, 597
ZraI GACGTC 1 cut(s) 1651
ZrmI AGTACT 1 cut(s) 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.