MD12G1228300.v1.1

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
30366708 .. 30371578
4871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1228300.v1.1.491

Sequence Viewer

Length: 1770 bp
ATGGGAGGATGCTTTTCGGACGTGAGGGGAGGCAAGGACGCCGTGGGCGGAGCCCTGCCAAGGCCCACCGAACCGAACGCCAAGAACGACGGCGCTCACAACGATGCCATTGAATTCTTTTACAGGGCGCATGGTGCTTACCCGCTTTTTACGCAACTCGAGTTATCCTTTTCAGCATCCAAGCTGCTTGATCTTGATGTCACTTCAAAGAGTGATCCTATGGTGGTTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTCGGGCGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGATGGTGCAGAATTTGATATTCCGTGTCTACGATGTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCAAGAGTTTCTTGGAGAAGGCAGTTGTATCCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTTAACATTGACTCTGAAAGGCAAAAACGGGCTTGGAGGTTTGAGAAACTTTGGAACACTCACCGTGCATGCCGAGGAAACTATTGCTTCTAGGAGTGCTGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACAAAGATCTGTTTTCTAAAAGCGATCCTTTCTTAAGAATATCAAGAACTGTTGAGACTGGGGGTTCTATTCCAATTTGCAAGACTGAGGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACACCCCACTGGTTATTGAGTGTTTTGATTTCAACAGCAATGGCAACCACATTCTTATTGGGAAACTCCAAAAATCAGTTGCAGACCTTGAAAAACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAAGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACAGGTTCAAATGGAGATCCTAGAAAGCCCGAGTCTTTGCACTATATTGATCCTTATGGCCGGCTGAATTCTTACCAGCAGGCTATAACAGAAGTAGGACAAGTCATTCAGTTCTACGATGCTGATAAGAGTTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCACACTGCTTCAACTTGAATGGAAATGCAAGTGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAATGCTCTACGCAATGTTGCTCTGTCAGGACCGACGTTGTTTGGCCAAGTGGTTAACAGGGCTGCTGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAACAAGTACTACGTCTTGCTCATTATAACGGATGGAATCCTCACTGACCTTCAAGAAACAAAAGATGCTTTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTTGGTAATGCGGATTTCAAACAAATGGAGATCCTTGACGCTGACAACGGGCAACGATTAGAAAGTTCTACAGGTCGCATAGCTACGCGTGACATTGTCCAGTTCGTTCCAATGAGAGATGTGCAGAGTGGGCAGATTTCCGTTGTTCAAGGTCTTTTGGAAGAGCTGCCCGGACAGTTTTTGAGTTACATGCGTTCCATGGACATCAAGCCGCTCACCTTCAATGCCGCTGCCCAAGCATCATCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

590

Amino Acids

64.8

Weight (kDa)

5.55

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 58 - 162 6.9e-12 C2 domain
C2 PF00168 202 - 291 6e-12 C2 domain
Copine PF07002 362 - 577 7.2e-81 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AccBSI CCGCTC 1 cut(s) 1732
AccI GTMKAC 2 cut(s) 231, 369
AccII CGCG 1 cut(s) 1606
AciI CCGC 8 cut(s) 48, 143, 604, 926, 1380, 1529, 1730, 1746
AclWI GGATC 7 cut(s) 209, 432, 641, 972, 1061, 1094, 1543
AcoI YGGCCR 2 cut(s) 1108, 1348
AcsI RAATTY 5 cut(s) 113, 352, 415, 599, 1117
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 399, 1412
AfiI CCNNNNNNNGG 2 cut(s) 60, 1193
AflII CTTAAG 1 cut(s) 655
AflIII ACRYGT 1 cut(s) 1604
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 737, 1382
AluBI AGCT 8 cut(s) 184, 256, 329, 955, 993, 1024, 1601, 1684
AluI AGCT 8 cut(s) 184, 256, 329, 955, 993, 1024, 1601, 1684
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 7 cut(s) 209, 432, 641, 972, 1061, 1094, 1543
Ama87I CYCGRG 3 cut(s) 158, 266, 1079
AoxI GGCC 3 cut(s) 62, 1108, 1348
ApeKI GCWGC 5 cut(s) 184, 1295, 1367, 1684, 1748
ApoI RAATTY 5 cut(s) 113, 352, 415, 599, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
AspLEI GCGC 2 cut(s) 95, 130
AspS9I GGNCC 3 cut(s) 63, 1334, 1386
AsuC2I CCSGG 1 cut(s) 1689
AsuHPI GGTGA 4 cut(s) 541, 910, 1489, 1726
AvaI CYCGRG 3 cut(s) 158, 266, 1079
AvaII GGWCC 2 cut(s) 1334, 1386
BaeGI GKGCMC 1 cut(s) 613
BalI TGGCCA 1 cut(s) 1350
BanII GRGCYC 1 cut(s) 55
BauI CACGAG 1 cut(s) 921
BbvI GCAGC 5 cut(s) 171, 1282, 1354, 1671, 1735
BccI CCATC 4 cut(s) 242, 337, 922, 1430
BceAI ACGGC 3 cut(s) 26, 106, 1227
BciT130I CCWGG 2 cut(s) 739, 1384
BciVI GTATCC 2 cut(s) 303, 464
BcnI CCSGG 1 cut(s) 1689
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 4 cut(s) 257, 579, 1011, 1071
BfmI CTRYAG 2 cut(s) 588, 1587
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 655
BfuI GTATCC 2 cut(s) 303, 464
BglII AGATCT 1 cut(s) 628
BisI GCNGC 8 cut(s) 185, 1296, 1368, 1380, 1685, 1730, 1746, 1749
BlsI GCNGC 8 cut(s) 186, 1297, 1369, 1381, 1686, 1731, 1747, 1750
BmcAI AGTACT 1 cut(s) 1412
Bme1390I CCNGG 3 cut(s) 739, 1384, 1689
Bme18I GGWCC 2 cut(s) 1334, 1386
BmeT110I CYCGRG 3 cut(s) 158, 266, 1079
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 63, 1334, 1386
BmiI GGNNCC 2 cut(s) 52, 1388
BmrFI CCNGG 3 cut(s) 739, 1384, 1689
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 6 cut(s) 94, 185, 1159, 1459, 1493, 1766
BmuI ACTGGG 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 1689
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 42, 59, 561, 1716
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 1193
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 3 cut(s) 685, 795, 1618
Bse3DI GCAATG 2 cut(s) 826, 1324
BseBI CCWGG 2 cut(s) 739, 1384
BseDI CCNNGG 4 cut(s) 42, 59, 561, 1716
BseGI GGATG 3 cut(s) 14, 176, 1441
BseLI CCNNNNNNNGG 2 cut(s) 60, 1193
BseMI GCAATG 2 cut(s) 826, 1324
BseMII CTCAG 3 cut(s) 699, 746, 1257
BseNI ACTGG 3 cut(s) 685, 795, 1618
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 5 cut(s) 171, 1282, 1354, 1671, 1735
BsgI GTGCAG 2 cut(s) 368, 1661
Bsh1236I CGCG 1 cut(s) 1606
BshFI GGCC 3 cut(s) 64, 1110, 1350
BsiHKCI CYCGRG 3 cut(s) 158, 266, 1079
BsiSI CCGG 3 cut(s) 302, 1111, 1689
BslFI GGGAC 1 cut(s) 1372
BslI CCNNNNNNNGG 2 cut(s) 60, 1193
BsmAI GTCTC 1 cut(s) 671
BsmFI GGGAC 1 cut(s) 1372
BsnI GGCC 3 cut(s) 64, 1110, 1350
BsoBI CYCGRG 3 cut(s) 158, 266, 1079
Bsp1286I GDGCHC 2 cut(s) 55, 613
Bsp143I GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
Bsp19I CCATGG 1 cut(s) 1716
BspACI CCGC 8 cut(s) 48, 143, 604, 926, 1380, 1529, 1730, 1746
BspANI GGCC 3 cut(s) 64, 1110, 1350
BspCNI CTCAG 3 cut(s) 700, 747, 1258
BspFNI CGCG 1 cut(s) 1606
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 52, 1388
BspPI GGATC 7 cut(s) 209, 432, 641, 972, 1061, 1094, 1543
BspQI GCTCTTC 1 cut(s) 1674
BspTI CTTAAG 1 cut(s) 655
BsrBI CCGCTC 1 cut(s) 1732
BsrDI GCAATG 2 cut(s) 826, 1324
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 3 cut(s) 685, 795, 1618
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 4 cut(s) 42, 59, 561, 1716
BssMI GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
BssNAI GTATAC 1 cut(s) 232
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 921
BssT1I CCWWGG 2 cut(s) 59, 1716
Bst1107I GTATAC 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 921
Bst2UI CCWGG 2 cut(s) 739, 1384
Bst4CI ACNGT 5 cut(s) 553, 673, 768, 1225, 1695
Bst6I CTCTTC 1 cut(s) 1674
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 1763
BstC8I GCNNGC 3 cut(s) 558, 1112, 1131
BstDEI CTNAG 3 cut(s) 708, 755, 1266
BstDSI CCRYGG 2 cut(s) 42, 1716
BstF5I GGATG 3 cut(s) 14, 176, 1441
BstFNI CGCG 1 cut(s) 1606
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 130
BstKTI GATC 9 cut(s) 193, 217, 427, 631, 649, 967, 1069, 1102, 1551
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
BstMWI GCNNNNNNNGC 7 cut(s) 134, 151, 1301, 1376, 1648, 1754, 1763
BstNI CCWGG 2 cut(s) 739, 1384
BstNSI RCATGY 2 cut(s) 560, 1711
BstSCI CCNGG 3 cut(s) 737, 1382, 1687
BstSFI CTRYAG 2 cut(s) 588, 1587
BstSLI GKGCMC 1 cut(s) 613
BstUI CGCG 1 cut(s) 1606
BstV1I GCAGC 5 cut(s) 171, 1282, 1354, 1671, 1735
BstX2I RGATCY 3 cut(s) 628, 1066, 1548
BstYI RGATCY 3 cut(s) 628, 1066, 1548
BstZ17I GTATAC 1 cut(s) 232
BsuI GTATCC 2 cut(s) 303, 464
BsuRI GGCC 3 cut(s) 64, 1110, 1350
BtgI CCRYGG 2 cut(s) 42, 1716
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 14, 176, 1441
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 3 cut(s) 788, 1231, 1446
Cac8I GCNNGC 3 cut(s) 558, 1112, 1131
CciI TCATGA 1 cut(s) 931
CfoI GCGC 2 cut(s) 95, 130
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 3 cut(s) 63, 1334, 1386
CseI GACGC 2 cut(s) 47, 1565
Csp6I GTAC 2 cut(s) 398, 1411
CviAII CATG 6 cut(s) 131, 557, 932, 1033, 1708, 1717
CviQI GTAC 2 cut(s) 398, 1411
DdeI CTNAG 3 cut(s) 708, 755, 1266
DpnI GATC 9 cut(s) 192, 216, 426, 630, 648, 966, 1068, 1101, 1550
DpnII GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
DraI TTTAAA 2 cut(s) 297, 729
EaeI YGGCCR 2 cut(s) 1108, 1348
Eam1104I CTCTTC 1 cut(s) 1674
EarI CTCTTC 1 cut(s) 1674
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 59, 1716
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 1334, 1386
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 3 cut(s) 158, 266, 1079
EcoO109I RGGNCCY 1 cut(s) 1386
EcoRI GAATTC 2 cut(s) 113, 1117
EcoRII CCWGG 2 cut(s) 737, 1382
EcoT14I CCWWGG 2 cut(s) 59, 1716
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 59, 1716
FaeI CATG 6 cut(s) 134, 560, 935, 1036, 1711, 1720
FalI AAGNNNNNCTT 4 cut(s) 420, 452, 634, 666
FaqI GGGAC 1 cut(s) 1372
FatI CATG 6 cut(s) 130, 556, 931, 1032, 1707, 1716
FauI CCCGC 2 cut(s) 150, 919
FblI GTMKAC 2 cut(s) 231, 369
Fnu4HI GCNGC 8 cut(s) 185, 1296, 1368, 1380, 1685, 1730, 1746, 1749
FokI GGATG 3 cut(s) 21, 163, 1448
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 8 cut(s) 185, 1296, 1368, 1380, 1685, 1730, 1746, 1749
FspBI CTAG 4 cut(s) 257, 579, 1011, 1071
GlaI GCGC 2 cut(s) 94, 129
GluI GCNGC 8 cut(s) 185, 1296, 1368, 1380, 1685, 1730, 1746, 1749
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 64, 1110, 1350
HapII CCGG 3 cut(s) 302, 1111, 1689
HgaI GACGC 2 cut(s) 47, 1565
HhaI GCGC 2 cut(s) 95, 130
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 134, 560, 935, 1036, 1711, 1720
Hin6I GCGC 2 cut(s) 93, 128
HinP1I GCGC 2 cut(s) 93, 128
HincII GTYRAC 2 cut(s) 1000, 1360
HindII GTYRAC 2 cut(s) 1000, 1360
HinfI GANTC 5 cut(s) 409, 499, 1082, 1262, 1440
HpaI GTTAAC 1 cut(s) 1360
HpaII CCGG 3 cut(s) 302, 1111, 1689
HphI GGTGA 4 cut(s) 541, 910, 1489, 1726
Hpy166II GTNNAC 5 cut(s) 232, 370, 901, 1000, 1360
Hpy188I TCNGA 7 cut(s) 19, 414, 463, 504, 773, 1267, 1277
Hpy188III TCNNGA 8 cut(s) 194, 238, 340, 428, 666, 932, 1332, 1457
Hpy8I GTNNAC 5 cut(s) 232, 370, 901, 1000, 1360
Hpy99I CGWCG 2 cut(s) 92, 1342
HpyAV CCTTC 6 cut(s) 437, 940, 1200, 1277, 1463, 1747
HpyCH4III ACNGT 5 cut(s) 553, 673, 768, 1225, 1695
HpyCH4IV ACGT 3 cut(s) 21, 1340, 1416
HpyCH4V TGCA 9 cut(s) 349, 556, 702, 763, 863, 1040, 1090, 1256, 1642
HpyF10VI GCNNNNNNNGC 7 cut(s) 134, 151, 1301, 1376, 1648, 1754, 1763
HpyF3I CTNAG 3 cut(s) 708, 755, 1266
HpySE526I ACGT 3 cut(s) 21, 1340, 1416
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 134, 560, 935, 1036, 1711, 1720
HspAI GCGC 2 cut(s) 93, 128
KroI GCCGGC 1 cut(s) 1110
KroNI GCCGGC 1 cut(s) 1112
KspAI GTTAAC 1 cut(s) 1360
Kzo9I GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
LguI GCTCTTC 1 cut(s) 1674
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 5 cut(s) 171, 1282, 1354, 1671, 1735
LweI GCATC 6 cut(s) 94, 185, 1159, 1459, 1493, 1766
MaeI CTAG 4 cut(s) 257, 579, 1011, 1071
MaeII ACGT 3 cut(s) 21, 1340, 1416
MaeIII GTNAC 4 cut(s) 199, 469, 1607, 1703
MalI GATC 9 cut(s) 192, 216, 426, 630, 648, 966, 1068, 1101, 1550
MbiI CCGCTC 1 cut(s) 1732
MboI GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
MboII GAAGA 4 cut(s) 253, 909, 1484, 1691
MflI RGATCY 3 cut(s) 628, 1066, 1548
MhlI GDGCHC 2 cut(s) 55, 613
MlsI TGGCCA 1 cut(s) 1350
MluI ACGCGT 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 1350
MlyI GAGTC 3 cut(s) 403, 493, 1091
MmeI TCCRAC 3 cut(s) 1255, 1422, 1495
Mox20I TGGCCA 1 cut(s) 1350
MroNI GCCGGC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 1350
MseI TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 1350
MspA1I CMGCKG 2 cut(s) 606, 1748
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 3 cut(s) 302, 1111, 1689
MspR9I CCNGG 3 cut(s) 739, 1384, 1689
MvaI CCWGG 2 cut(s) 739, 1384
MvnI CGCG 1 cut(s) 1606
MwoI GCNNNNNNNGC 7 cut(s) 134, 151, 1301, 1376, 1648, 1754, 1763
NaeI GCCGGC 1 cut(s) 1112
NciI CCSGG 1 cut(s) 1689
NcoI CCATGG 1 cut(s) 1716
NdeII GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
NgoMIV GCCGGC 1 cut(s) 1110
NlaIII CATG 6 cut(s) 134, 560, 935, 1036, 1711, 1720
NlaIV GGNNCC 2 cut(s) 52, 1388
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 3 cut(s) 199, 469, 1607
NspI RCATGY 2 cut(s) 560, 1711
PaeI GCATGC 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 1674
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 1112
PfeI GAWTC 2 cut(s) 1262, 1440
PflFI GACNNNGTC 1 cut(s) 1613
PkrI GCNGC 8 cut(s) 186, 1297, 1369, 1381, 1686, 1731, 1747, 1750
PleI GAGTC 3 cut(s) 403, 493, 1090
PpsI GAGTC 3 cut(s) 403, 493, 1090
PpuMI RGGWCCY 1 cut(s) 1386
PsiI TTATAA 1 cut(s) 1430
Psp5II RGGWCCY 1 cut(s) 1386
Psp6I CCWGG 2 cut(s) 737, 1382
PspGI CCWGG 2 cut(s) 737, 1382
PspN4I GGNNCC 2 cut(s) 52, 1388
PspPI GGNCC 3 cut(s) 63, 1334, 1386
PspPPI RGGWCCY 1 cut(s) 1386
PspXI VCTCGAGB 1 cut(s) 158
PsuI RGATCY 3 cut(s) 628, 1066, 1548
PsyI GACNNNGTC 1 cut(s) 1613
RsaI GTAC 2 cut(s) 399, 1412
RsaNI GTAC 2 cut(s) 398, 1411
RseI CAYNNNNRTG 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 1674
SaqAI TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
SatI GCNGC 8 cut(s) 185, 1296, 1368, 1380, 1685, 1730, 1746, 1749
Sau3AI GATC 9 cut(s) 190, 214, 424, 628, 646, 964, 1066, 1099, 1548
Sau96I GGNCC 3 cut(s) 63, 1334, 1386
ScaI AGTACT 1 cut(s) 1412
SchI GAGTC 3 cut(s) 403, 493, 1091
ScrFI CCNGG 3 cut(s) 739, 1384, 1689
SduI GDGCHC 2 cut(s) 55, 613
SfaNI GCATC 6 cut(s) 94, 185, 1159, 1459, 1493, 1766
SfcI CTRYAG 2 cut(s) 588, 1587
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 2 cut(s) 1334, 1386
SlaI CTCGAG 1 cut(s) 158
SmiI ATTTAAAT 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 158, 236, 655
SmoI CTYRAG 3 cut(s) 158, 236, 655
SphI GCATGC 1 cut(s) 560
SsiI CCGC 8 cut(s) 48, 143, 604, 926, 1380, 1529, 1730, 1746
SspMI CTAG 4 cut(s) 257, 579, 1011, 1071
StyD4I CCNGG 3 cut(s) 737, 1382, 1687
StyI CCWWGG 2 cut(s) 59, 1716
SwaI ATTTAAAT 1 cut(s) 297
TaaI ACNGT 5 cut(s) 553, 673, 768, 1225, 1695
TaiI ACGT 3 cut(s) 24, 1343, 1419
TaqI TCGA 3 cut(s) 159, 339, 484
TaqII GACCGA 1 cut(s) 1351
TatI WGTACW 1 cut(s) 1410
TauI GCSGC 3 cut(s) 1382, 1732, 1748
TfiI GAWTC 2 cut(s) 1262, 1440
Tru1I TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
Tru9I TTAA 7 cut(s) 296, 491, 597, 656, 728, 1026, 1359
TscAI CASTG 3 cut(s) 795, 1238, 1453
TseFI GTSAC 3 cut(s) 199, 469, 1607
TseI GCWGC 5 cut(s) 184, 1295, 1367, 1684, 1748
Tsp45I GTSAC 3 cut(s) 199, 469, 1607
TspDTI ATGAA 2 cut(s) 948, 1021
TspGWI ACGGA 3 cut(s) 353, 1448, 1648
TspRI CASTG 3 cut(s) 795, 1238, 1453
Tth111I GACNNNGTC 1 cut(s) 1613
Vha464I CTTAAG 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 1334, 1386
XapI RAATTY 5 cut(s) 113, 352, 415, 599, 1117
XceI RCATGY 2 cut(s) 560, 1711
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 231, 369
XspI CTAG 4 cut(s) 257, 579, 1011, 1071
ZrmI AGTACT 1 cut(s) 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.