Rmu_sc0003983.1_g000002

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003983.1
OG0000862
Physical Location & Seq
Reverse (-)
2325 .. 7427
5103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003983.1_g000002.1.cds

Sequence Viewer

Length: 1752 bp
atgggagggtgtttttctgacgtgaaaggtggcaagcaggccgtgggtggggcccgtcatcaacaacagagacccatcaacagcgacgccgttgagctctttaaccgctcccgaggggtttatccactctacactccgctcgagttatcgctgtcatcaactaacttgcttgatcgtgacattacgtcaaagagtgatcccatggttgtggtttatgtaaagagaggagatggtaagctagaggaactaggtagaactgaagtaatattgaatagtttgaatccagcgtggattgagaaaattacagtttcatatcagtttgaggttgtgcaaccattgatattccacgtctatgatgtcgatacaaagtatcacaacgtaaatgtaaagacactgaagttggaggatcaagaatttcttggggaaggcagttgtgttctttcagagatagtgaccaaacaaagtcgaagtttaaccttgaatctgcatagcagaagtgggcgtggaggtttgagagacttcggaacactcactgtccatgctgaagaaactatggcttcaaagagtgttgttgagataaaattccgctgttcccagttggaaaacaaagacgtcttctctaaaagcgatcctttcttaagagtatctagaattgttgagagtggaggttctgttccaatttgcaagactgaagtggtgtataacaatttaaatccaatctggaaaccactatccctgagtatgcagcagtttggaagcaaggataatccattagtcatcgagtgttttgatttcaacagcaatggcaatcatgttcttattgggaaacttcaaaaatcagtggcagacctggagagattatacaaagaaagaagtggggtgaatttcgtcattccatcttctcatggtcatgaaaaggatttaaagggtcagttgtttgtggatcaatttaatgaaaagcagcagttcagctttctcgattatatttccagtggatttgagcttaacttcatggttgctgttgactttacggcttcaaatggaaatcctcatcaacatgattcattgcactacattgatccgtatggccggttgaattcttaccagcaggcaataacggaagttggggaagtcattcagttttatgatgctgataaacggtttccggcttggggctttggaggaaggacagctggaggacctgtatcgcactgttttaacttggatggaactgcaactggctttgaggttgaaggagttcaaggcataatgactgcttatgccagtgctctacacaatgtttctctatcaggacccactttgtttggcccagtggttaatggggctgcagagattgctggcaattctctattccacaacaacaataagtactatgtgctaatgatcataacggatgggatacttacagacctgcaggaaacaacagatgctttcgtgagggcatctgatcttcccctctcagttcttatagttggagtaggaagtgcggatttcaaacaaatggagattctcgatgctgacaatggactgcgattgtcaagttcaacaggtcgtgtggctaccagagacattgtacaatttgttcctatgagagaagtgcagagtgggcgcatttctgctgttcaatcgcttctcgaagagctacctggacagttcttgagttacatgcgctggagagacgtcatgcctctcactgtccatgcagcccaatcatctgctccacaactttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

583

Amino Acids

64.48

Weight (kDa)

5.65

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 615, 1704
Acc36I ACCTGC 1 cut(s) 1440
AccBSI CCGCTC 2 cut(s) 108, 139
AciI CCGC 4 cut(s) 106, 137, 586, 1508
AclWI GGATC 5 cut(s) 191, 414, 623, 951, 1073
AcoI YGGCCR 1 cut(s) 1087
AcsI RAATTY 4 cut(s) 413, 581, 883, 1096
AcuI CTGAAG 4 cut(s) 279, 416, 564, 711
AcyI GRCGYC 3 cut(s) 87, 612, 1701
AfaI GTAC 2 cut(s) 1391, 1596
AfiI CCNNNNNNNGG 2 cut(s) 48, 1172
AflII CTTAAG 1 cut(s) 637
AhdI GACNNNNNGTC 1 cut(s) 184
AjiI CACGTC 2 cut(s) 22, 349
AjnI CCWGG 2 cut(s) 849, 1666
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
AluBI AGCT 6 cut(s) 97, 238, 972, 1003, 1193, 1663
AluI AGCT 6 cut(s) 97, 238, 972, 1003, 1193, 1663
Alw21I GWGCWC 2 cut(s) 99, 1291
Alw26I GTCTC 4 cut(s) 64, 510, 1581, 1692
AlwI GGATC 5 cut(s) 191, 414, 623, 951, 1073
Ama87I CYCGRG 2 cut(s) 111, 140
AoxI GGCC 4 cut(s) 39, 51, 1087, 1327
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 4 cut(s) 745, 961, 1346, 1724
ApoI RAATTY 4 cut(s) 413, 581, 883, 1096
Asp700I GAANNNNTTC 2 cut(s) 1134, 1257
AspLEI GCGC 2 cut(s) 1632, 1692
AspS9I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
AsuHPI GGTGA 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 111, 140
AvaII GGWCC 2 cut(s) 1199, 1313
BaeGI GKGCMC 1 cut(s) 55
BanII GRGCYC 2 cut(s) 55, 99
BbsI GAAGAC 1 cut(s) 607
Bbv12I GWGCWC 2 cut(s) 99, 1291
BbvI GCAGC 4 cut(s) 757, 973, 1333, 1736
BccI CCATC 5 cut(s) 83, 224, 904, 1220, 1409
BceAI ACGGC 3 cut(s) 26, 74, 1047
BciT130I CCWGG 2 cut(s) 851, 1668
BciVI GTATCC 1 cut(s) 1413
BclI TGATCA 1 cut(s) 1404
BcoDI GTCTC 4 cut(s) 64, 510, 1581, 1692
BfaI CTAG 3 cut(s) 239, 248, 648
BfmI CTRYAG 2 cut(s) 1347, 1433
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 1440
BfuI GTATCC 1 cut(s) 1413
BisI GCNGC 4 cut(s) 746, 962, 1347, 1725
BlsI GCNGC 4 cut(s) 747, 963, 1348, 1726
BmcAI AGTACT 1 cut(s) 1391
Bme1390I CCNGG 2 cut(s) 851, 1668
Bme18I GGWCC 2 cut(s) 1199, 1313
BmeRI GACNNNNNGTC 1 cut(s) 184
BmeT110I CYCGRG 2 cut(s) 111, 140
BmgBI CACGTC 2 cut(s) 22, 349
BmgT120I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
BmiI GGNNCC 3 cut(s) 52, 53, 1315
BmrFI CCNGG 2 cut(s) 851, 1668
BmrI ACTGGG 2 cut(s) 589, 1325
BmsI GCATC 4 cut(s) 1138, 1438, 1472, 1525
BmuI ACTGGG 2 cut(s) 589, 1325
BpiI GAAGAC 1 cut(s) 607
BpmI CTGGAG 3 cut(s) 872, 1215, 1714
BpuEI CTTGAG 1 cut(s) 1699
BsaHI GRCGYC 3 cut(s) 87, 612, 1701
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 3 cut(s) 42, 112, 201
BsaXI ACNNNNNCTCC 4 cut(s) 657, 687, 1188, 1218
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 1172
Bse118I RCCGGY 1 cut(s) 1089
Bse1I ACTGG 5 cut(s) 595, 990, 1243, 1284, 1331
Bse3DI GCAATG 2 cut(s) 808, 1064
BseBI CCWGG 2 cut(s) 851, 1668
BseDI CCNNGG 3 cut(s) 42, 112, 201
BseGI GGATG 2 cut(s) 1231, 1420
BseLI CCNNNNNNNGG 2 cut(s) 48, 1172
BseMI GCAATG 2 cut(s) 808, 1064
BseMII CTCAG 2 cut(s) 728, 1494
BseNI ACTGG 5 cut(s) 595, 990, 1243, 1284, 1331
BseRI GAGGAG 1 cut(s) 240
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 4 cut(s) 757, 973, 1333, 1736
BsgI GTGCAG 1 cut(s) 1640
BshFI GGCC 4 cut(s) 41, 53, 1089, 1329
BsiHKAI GWGCWC 2 cut(s) 99, 1291
BsiHKCI CYCGRG 2 cut(s) 111, 140
BsiSI CCGG 2 cut(s) 1090, 1166
BslI CCNNNNNNNGG 2 cut(s) 48, 1172
BsmAI GTCTC 4 cut(s) 64, 510, 1581, 1692
BsmBI CGTCTC 1 cut(s) 1692
BsnI GGCC 4 cut(s) 41, 53, 1089, 1329
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 2 cut(s) 111, 140
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 3 cut(s) 55, 99, 1291
Bsp1407I TGTACA 1 cut(s) 1594
Bsp143I GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 4 cut(s) 106, 137, 586, 1508
BspANI GGCC 4 cut(s) 41, 53, 1089, 1329
BspCNI CTCAG 2 cut(s) 729, 1493
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 3 cut(s) 52, 53, 1315
BspMAI CTGCAG 2 cut(s) 1351, 1437
BspMI ACCTGC 1 cut(s) 1440
BspPI GGATC 5 cut(s) 191, 414, 623, 951, 1073
BspQI GCTCTTC 1 cut(s) 1653
BspTI CTTAAG 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 64
BsrBI CCGCTC 2 cut(s) 108, 139
BsrDI GCAATG 2 cut(s) 808, 1064
BsrFI RCCGGY 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 1594
BsrI ACTGG 5 cut(s) 595, 990, 1243, 1284, 1331
BssAI RCCGGY 1 cut(s) 1089
BssECI CCNNGG 3 cut(s) 42, 112, 201
BssMI GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
BssNI GRCGYC 3 cut(s) 87, 612, 1701
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 851, 1668
Bst4CI ACNGT 6 cut(s) 307, 535, 1161, 1214, 1674, 1717
Bst6I CTCTTC 1 cut(s) 1653
BstACI GRCGYC 3 cut(s) 87, 612, 1701
BstAFI CTTAAG 1 cut(s) 637
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 1594
BstC8I GCNNGC 4 cut(s) 35, 39, 1110, 1360
BstDEI CTNAG 2 cut(s) 737, 1480
BstDSI CCRYGG 2 cut(s) 42, 201
BstF5I GGATG 2 cut(s) 1231, 1420
BstHHI GCGC 2 cut(s) 1632, 1692
BstKTI GATC 8 cut(s) 175, 199, 409, 631, 946, 1081, 1407, 1471
BstMAI GTCTC 4 cut(s) 64, 510, 1581, 1692
BstMBI GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
BstMWI GCNNNNNNNGC 2 cut(s) 1355, 1627
BstNI CCWGG 2 cut(s) 851, 1668
BstNSI RCATGY 1 cut(s) 1690
BstSCI CCNGG 2 cut(s) 849, 1666
BstSFI CTRYAG 2 cut(s) 1347, 1433
BstSLI GKGCMC 1 cut(s) 55
BstV1I GCAGC 4 cut(s) 757, 973, 1333, 1736
BstV2I GAAGAC 1 cut(s) 607
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 1413
BsuRI GGCC 4 cut(s) 41, 53, 1089, 1329
BtgI CCRYGG 2 cut(s) 42, 201
BtrI CACGTC 2 cut(s) 22, 349
BtsCI GGATG 2 cut(s) 1231, 1420
BtsIMutI CAGTG 8 cut(s) 392, 531, 846, 997, 1210, 1291, 1338, 1713
BveI ACCTGC 1 cut(s) 1440
Cac8I GCNNGC 4 cut(s) 35, 39, 1110, 1360
CciI TCATGA 1 cut(s) 910
CfoI GCGC 2 cut(s) 1632, 1692
Cfr10I RCCGGY 1 cut(s) 1089
Cfr13I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
CseI GACGC 1 cut(s) 95
Csp6I GTAC 2 cut(s) 1390, 1595
CspCI CAANNNNNGTGG 2 cut(s) 1306, 1341
CviQI GTAC 2 cut(s) 1390, 1595
DdeI CTNAG 2 cut(s) 737, 1480
DpnI GATC 8 cut(s) 174, 198, 408, 630, 945, 1080, 1406, 1470
DpnII GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
DraI TTTAAA 2 cut(s) 711, 924
DriI GACNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 1087
Eam1104I CTCTTC 1 cut(s) 1653
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 1653
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 55, 99
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 1199, 1313
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 4 cut(s) 279, 416, 564, 711
Eco88I CYCGRG 2 cut(s) 111, 140
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 3 cut(s) 51, 1199, 1313
EcoRI GAATTC 1 cut(s) 1096
EcoRII CCWGG 2 cut(s) 849, 1666
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 55, 99
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 1692
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 616, 648
FbaI TGATCA 1 cut(s) 1404
Fnu4HI GCNGC 4 cut(s) 746, 962, 1347, 1725
FokI GGATG 2 cut(s) 1238, 1427
FriOI GRGCYC 2 cut(s) 55, 99
Fsp4HI GCNGC 4 cut(s) 746, 962, 1347, 1725
FspBI CTAG 3 cut(s) 239, 248, 648
GlaI GCGC 2 cut(s) 1631, 1691
GluI GCNGC 4 cut(s) 746, 962, 1347, 1725
GsuI CTGGAG 3 cut(s) 872, 1215, 1714
HaeIII GGCC 4 cut(s) 41, 53, 1089, 1329
HapII CCGG 2 cut(s) 1090, 1166
HgaI GACGC 1 cut(s) 95
HhaI GCGC 2 cut(s) 1632, 1692
Hin1I GRCGYC 3 cut(s) 87, 612, 1701
Hin6I GCGC 2 cut(s) 1630, 1690
HinP1I GCGC 2 cut(s) 1630, 1690
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HinfI GANTC 4 cut(s) 280, 481, 1061, 1528
HpaII CCGG 2 cut(s) 1090, 1166
HphI GGTGA 1 cut(s) 892
Hpy166II GTNNAC 1 cut(s) 1024
Hpy188I TCNGA 4 cut(s) 19, 445, 524, 1468
Hpy8I GTNNAC 1 cut(s) 1024
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 3 cut(s) 419, 1179, 1247
HpyCH4III ACNGT 6 cut(s) 307, 535, 1161, 1214, 1674, 1717
HpyCH4IV ACGT 6 cut(s) 21, 185, 348, 378, 612, 1701
HpyF10VI GCNNNNNNNGC 2 cut(s) 1355, 1627
HpyF3I CTNAG 2 cut(s) 737, 1480
HpySE526I ACGT 6 cut(s) 21, 185, 348, 378, 612, 1701
Hsp92I GRCGYC 3 cut(s) 87, 612, 1701
HspAI GCGC 2 cut(s) 1630, 1690
Ksp22I TGATCA 1 cut(s) 1404
Kzo9I GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
LguI GCTCTTC 1 cut(s) 1653
LmnI GCTCC 2 cut(s) 113, 1744
Lsp1109I GCAGC 4 cut(s) 757, 973, 1333, 1736
LweI GCATC 4 cut(s) 1138, 1438, 1472, 1525
MaeI CTAG 3 cut(s) 239, 248, 648
MaeII ACGT 6 cut(s) 21, 185, 348, 378, 612, 1701
MaeIII GTNAC 3 cut(s) 176, 451, 1682
MalI GATC 8 cut(s) 174, 198, 408, 630, 945, 1080, 1406, 1470
MbiI CCGCTC 2 cut(s) 108, 139
MboI GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
MboII GAAGA 5 cut(s) 557, 607, 891, 1463, 1670
MhlI GDGCHC 3 cut(s) 55, 99, 1291
MmeI TCCRAC 3 cut(s) 381, 579, 1474
MroXI GAANNNNTTC 2 cut(s) 1134, 1257
MseI TTAA 9 cut(s) 102, 473, 638, 710, 923, 951, 1005, 1218, 1338
MslI CAYNNNNRTG 4 cut(s) 206, 351, 909, 1056
MspA1I CMGCKG 2 cut(s) 588, 1193
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 2 cut(s) 1090, 1166
MspR9I CCNGG 2 cut(s) 851, 1668
MvaI CCWGG 2 cut(s) 851, 1668
MwoI GCNNNNNNNGC 2 cut(s) 1355, 1627
NcoI CCATGG 1 cut(s) 201
NdeII GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
NlaIV GGNNCC 3 cut(s) 52, 53, 1315
NmuCI GTSAC 2 cut(s) 176, 451
NspI RCATGY 1 cut(s) 1690
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 910
PciSI GCTCTTC 1 cut(s) 1653
PdmI GAANNNNTTC 2 cut(s) 1134, 1257
PfeI GAWTC 4 cut(s) 280, 481, 1061, 1528
PkrI GCNGC 4 cut(s) 747, 963, 1348, 1726
PpuMI RGGWCCY 2 cut(s) 1199, 1313
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 2 cut(s) 1199, 1313
Psp6I CCWGG 2 cut(s) 849, 1666
PspGI CCWGG 2 cut(s) 849, 1666
PspN4I GGNNCC 3 cut(s) 52, 53, 1315
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
PspPPI RGGWCCY 2 cut(s) 1199, 1313
PspXI VCTCGAGB 1 cut(s) 140
PsrI GAACNNNNNNTAC 2 cut(s) 1587, 1619
PstI CTGCAG 2 cut(s) 1351, 1437
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 2 cut(s) 1391, 1596
RsaNI GTAC 2 cut(s) 1390, 1595
RseI CAYNNNNRTG 4 cut(s) 206, 351, 909, 1056
SacI GAGCTC 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 1653
SaqAI TTAA 9 cut(s) 102, 473, 638, 710, 923, 951, 1005, 1218, 1338
SatI GCNGC 4 cut(s) 746, 962, 1347, 1725
Sau3AI GATC 8 cut(s) 172, 196, 406, 628, 943, 1078, 1404, 1468
Sau96I GGNCC 5 cut(s) 51, 52, 1199, 1313, 1328
SbfI CCTGCAGG 1 cut(s) 1437
ScaI AGTACT 1 cut(s) 1391
ScrFI CCNGG 2 cut(s) 851, 1668
SdaI CCTGCAGG 1 cut(s) 1437
SduI GDGCHC 3 cut(s) 55, 99, 1291
SfaNI GCATC 4 cut(s) 1138, 1438, 1472, 1525
SfcI CTRYAG 2 cut(s) 1347, 1433
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 2 cut(s) 1199, 1313
SlaI CTCGAG 1 cut(s) 140
SmiI ATTTAAAT 1 cut(s) 711
SmiMI CAYNNNNRTG 4 cut(s) 206, 351, 909, 1056
SmlI CTYRAG 3 cut(s) 140, 637, 1678
SmoI CTYRAG 3 cut(s) 140, 637, 1678
Sse8387I CCTGCAGG 1 cut(s) 1437
SsiI CCGC 4 cut(s) 106, 137, 586, 1508
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 239, 248, 648
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 849, 1666
StyI CCWWGG 1 cut(s) 201
SwaI ATTTAAAT 1 cut(s) 711
TaaI ACNGT 6 cut(s) 307, 535, 1161, 1214, 1674, 1717
TaiI ACGT 6 cut(s) 24, 188, 351, 381, 615, 1704
TaqI TCGA 7 cut(s) 141, 360, 466, 780, 978, 1533, 1656
TatI WGTACW 2 cut(s) 1389, 1594
TfiI GAWTC 4 cut(s) 280, 481, 1061, 1528
Tru1I TTAA 9 cut(s) 102, 473, 638, 710, 923, 951, 1005, 1218, 1338
Tru9I TTAA 9 cut(s) 102, 473, 638, 710, 923, 951, 1005, 1218, 1338
TscAI CASTG 8 cut(s) 399, 538, 846, 997, 1217, 1291, 1338, 1720
TseFI GTSAC 2 cut(s) 176, 451
TseI GCWGC 4 cut(s) 745, 961, 1346, 1724
Tsp45I GTSAC 2 cut(s) 176, 451
TspDTI ATGAA 5 cut(s) 300, 927, 969, 1000, 1053
TspGWI ACGGA 3 cut(s) 1071, 1133, 1427
TspRI CASTG 8 cut(s) 399, 538, 846, 997, 1217, 1291, 1338, 1720
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 1199, 1313
XapI RAATTY 4 cut(s) 413, 581, 883, 1096
XbaI TCTAGA 1 cut(s) 647
XceI RCATGY 1 cut(s) 1690
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 2 cut(s) 1134, 1257
XspI CTAG 3 cut(s) 239, 248, 648
ZraI GACGTC 2 cut(s) 613, 1702
ZrmI AGTACT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.