RLG00000025522

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000862
Physical Location & Seq
Reverse (-)
46429792 .. 46435389
5598 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025522

Sequence Viewer

Length: 1764 bp
ATGGGAGGGTGTTTTTCCGACGTGAAAGGTGGCAAGCAGGCAGTGGGAAAGGCCCATCAACAACAGAGGCCGATCAACAAGGACGCTGACTCAAACGACGCCGTTGAGCTCTTTAACCGCTCTCGTGGGGTTTATCCTCTCTACACTCCGCTCGAGTTATCGCTGTCAGCAACTAACTTGCTTGATCGTGACATTATGTCAAAGAGTGATCCTATGGTTGTGGTTTATGTGAAGAAAGGAGATGGTAAGCTAGAGGAACTAGGGAGAACTGAAGTAATACTGAATAGTTTGAATCCAGCGTGGATTGAGAAAATCACAGTCTCATATCAGTTCGAGATTGTGCAGTCATTGATATTTCATGTCTATGATGTCGATACAAAATATCACAACGTAAATGTAAAGACACTGAAGTTGGAGGATCAAGAATTTCTTGGGGAAGGCAGTTGCGTTCTTTCAGAGATAGTGACCAAACAAAGTCGAAGTTTAACCTTGAATATGCATAACAGACGTGGGCGTGGAGGTTTGAGAAACTTAGGAACACTCACTGTCCATGCTGAAGAAACTATGGCTTCGAAGAGTGTTGTTGAGATAAAATTCCGTTGTTCCCAATTAGAAAACAAAGACCTCTTCTCTAAAAGTGATCCTTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTAGTGGATAACAATTTAAATCCAATTTGGAAACCACTATCGCTCAGTATGCAGCAGTTTGGAAGCAAGGATAATCCAGTAGTCATCGAGTGCTTTGATTTCAACAGCAATGGCAATCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCAGACCTGGAGAGATTATACAAAGAAAGAAGTGGGGTGAATTTTGTCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTTGTGGATCAATTTAATGAAAAGCAACAGTTCAGCTTTCTCGATTATATTTCCAGTGGATTTGAACTTAACTTCATGGTTGCTGTTGACTTTACGGCTTCAAATGGAAATCCTCAACAACATGATTCGTTGCACTACATTGATCCGTATGGCCGATTAAATTCTTACCAGCAGGCAGTAACAGAAGTTGGGGAAGTCATTCAGTTTTATGATACTGACAAACGCTTTCCTGCTTGGGGCTTTGGAGGAAGGACAGCTGGAGGACCTATATCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTTGAAGGGGTTCAAGGCATAATGACTGCGTATGCCAGTGCCCTACATAATGTTTCTCTATCAGGACCCACTTTGTTTGGCCCAGTATTTACTAGGGCTGCAGAGATTGCTGGCAATTCTCTATTCCACAACAACAATAAGTACTATGTGCTGCTGATCATAACGGATGGGATACTTACAGACCTGCAGGAAACAACAGATGCTTTGGTTCGGGCATCTGATCTTCCCCTCTCAGTTCTTATAGTTGGAGTGGGAAGTGCGGATTTCAAACAAATGGAGATTCTCGATGCTGACAATGGACAGCGATTATCAAGTTCAACAGGCCGTGTGGCTACCAGAGACATTGTACAATTTGTTCCTATGAGAGAAGTGCAGAGTGGGCGCATTTCTGCTGTTCAATCGCTTCTCGAAGAGCTGCCTGGACAGTTCTTGAGTTACATGCGCTGCAGAGACGTCATGCCTCTCACTGTCCATGCAGCCCAATCATCTGCTCCACAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

588

Amino Acids

64.95

Weight (kDa)

5.75

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 55 - 159 1.1e-12 C2 domain
C2 PF00168 200 - 289 4.4e-12 C2 domain
Copine PF07002 359 - 572 4.2e-75 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1716
Acc36I ACCTGC 1 cut(s) 1452
AccBSI CCGCTC 2 cut(s) 120, 151
AciI CCGC 3 cut(s) 118, 149, 1520
AclWI GGATC 5 cut(s) 203, 426, 635, 963, 1085
AcoI YGGCCR 1 cut(s) 1099
AcsI RAATTY 4 cut(s) 425, 593, 895, 1108
AcuI CTGAAG 4 cut(s) 291, 428, 576, 723
AcyI GRCGYC 2 cut(s) 99, 1713
AfaI GTAC 2 cut(s) 1403, 1608
AfiI CCNNNNNNNGG 1 cut(s) 1184
AflII CTTAAG 1 cut(s) 649
AhdI GACNNNNNGTC 1 cut(s) 196
AjiI CACGTC 2 cut(s) 22, 509
AjnI CCWGG 2 cut(s) 861, 1678
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 5 cut(s) 109, 250, 984, 1205, 1675
AluI AGCT 5 cut(s) 109, 250, 984, 1205, 1675
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 3 cut(s) 325, 1593, 1704
AlwI GGATC 5 cut(s) 203, 426, 635, 963, 1085
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 5 cut(s) 51, 68, 1099, 1339, 1582
ApeKI GCWGC 6 cut(s) 757, 1358, 1411, 1675, 1704, 1736
ApoI RAATTY 4 cut(s) 425, 593, 895, 1108
Asp700I GAANNNNTTC 2 cut(s) 1146, 1269
AspLEI GCGC 2 cut(s) 1644, 1704
AspS9I GGNCC 4 cut(s) 52, 1211, 1325, 1340
AsuHPI GGTGA 1 cut(s) 904
AsuII TTCGAA 1 cut(s) 572
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 1211, 1325
BaeGI GKGCMC 1 cut(s) 1303
BanII GRGCYC 1 cut(s) 111
BauI CACGAG 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 6 cut(s) 769, 1345, 1398, 1662, 1691, 1748
BccI CCATC 5 cut(s) 63, 236, 916, 1232, 1421
BceAI ACGGC 3 cut(s) 86, 1059, 1569
BciT130I CCWGG 2 cut(s) 863, 1680
BciVI GTATCC 1 cut(s) 1425
BclI TGATCA 1 cut(s) 1416
BcoDI GTCTC 3 cut(s) 325, 1593, 1704
BfaI CTAG 4 cut(s) 251, 260, 660, 1353
BfmI CTRYAG 3 cut(s) 1359, 1445, 1705
BfrI CTTAAG 1 cut(s) 649
BfuAI ACCTGC 1 cut(s) 1452
BfuI GTATCC 1 cut(s) 1425
BisI GCNGC 6 cut(s) 758, 1359, 1412, 1676, 1705, 1737
BlsI GCNGC 6 cut(s) 759, 1360, 1413, 1677, 1706, 1738
BmcAI AGTACT 1 cut(s) 1403
Bme1390I CCNGG 2 cut(s) 863, 1680
Bme18I GGWCC 2 cut(s) 1211, 1325
BmeRI GACNNNNNGTC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 152
BmgBI CACGTC 2 cut(s) 22, 509
BmgT120I GGNCC 4 cut(s) 52, 1211, 1325, 1340
BmiI GGNNCC 1 cut(s) 1327
BmrFI CCNGG 2 cut(s) 863, 1680
BmrI ACTGGG 1 cut(s) 1337
BmsI GCATC 3 cut(s) 1450, 1484, 1537
BmuI ACTGGG 1 cut(s) 1337
BpmI CTGGAG 2 cut(s) 884, 1227
Bpu14I TTCGAA 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 1711
BsaHI GRCGYC 2 cut(s) 99, 1713
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bsc4I CCNNNNNNNGG 1 cut(s) 1184
Bse1I ACTGG 5 cut(s) 782, 1002, 1255, 1296, 1343
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 2 cut(s) 863, 1680
BseGI GGATG 2 cut(s) 1243, 1432
BseLI CCNNNNNNNGG 1 cut(s) 1184
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 2 cut(s) 763, 1506
BseNI ACTGG 5 cut(s) 782, 1002, 1255, 1296, 1343
BseSI GKGCMC 1 cut(s) 1303
BseXI GCAGC 6 cut(s) 769, 1345, 1398, 1662, 1691, 1748
BsgI GTGCAG 2 cut(s) 362, 1652
BshFI GGCC 5 cut(s) 53, 70, 1101, 1341, 1584
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 1184
BsmAI GTCTC 3 cut(s) 325, 1593, 1704
BsmBI CGTCTC 1 cut(s) 1704
BsnI GGCC 5 cut(s) 53, 70, 1101, 1341, 1584
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 572
Bsp1286I GDGCHC 2 cut(s) 111, 1303
Bsp1407I TGTACA 1 cut(s) 1606
Bsp143I GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
BspACI CCGC 3 cut(s) 118, 149, 1520
BspANI GGCC 5 cut(s) 53, 70, 1101, 1341, 1584
BspCNI CTCAG 2 cut(s) 762, 1505
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 1 cut(s) 1327
BspMAI CTGCAG 3 cut(s) 1363, 1449, 1709
BspMI ACCTGC 1 cut(s) 1452
BspPI GGATC 5 cut(s) 203, 426, 635, 963, 1085
BspQI GCTCTTC 1 cut(s) 1665
BspT104I TTCGAA 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 649
BsrBI CCGCTC 2 cut(s) 120, 151
BsrDI GCAATG 1 cut(s) 820
BsrGI TGTACA 1 cut(s) 1606
BsrI ACTGG 5 cut(s) 782, 1002, 1255, 1296, 1343
BssMI GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
BssNI GRCGYC 2 cut(s) 99, 1713
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 863, 1680
Bst4CI ACNGT 6 cut(s) 319, 547, 978, 1226, 1686, 1729
Bst6I CTCTTC 3 cut(s) 569, 632, 1665
BstACI GRCGYC 2 cut(s) 99, 1713
BstAFI CTTAAG 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 1367
BstAUI TGTACA 1 cut(s) 1606
BstBI TTCGAA 1 cut(s) 572
BstC8I GCNNGC 4 cut(s) 35, 39, 1122, 1372
BstDEI CTNAG 3 cut(s) 532, 749, 1492
BstF5I GGATG 2 cut(s) 1243, 1432
BstHHI GCGC 2 cut(s) 1644, 1704
BstKTI GATC 9 cut(s) 75, 187, 211, 421, 643, 958, 1093, 1419, 1483
BstMAI GTCTC 3 cut(s) 325, 1593, 1704
BstMBI GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
BstMWI GCNNNNNNNGC 3 cut(s) 754, 1367, 1639
BstNI CCWGG 2 cut(s) 863, 1680
BstNSI RCATGY 1 cut(s) 1702
BstSCI CCNGG 2 cut(s) 861, 1678
BstSFI CTRYAG 3 cut(s) 1359, 1445, 1705
BstSLI GKGCMC 1 cut(s) 1303
BstV1I GCAGC 6 cut(s) 769, 1345, 1398, 1662, 1691, 1748
BsuI GTATCC 1 cut(s) 1425
BsuRI GGCC 5 cut(s) 53, 70, 1101, 1341, 1584
BtrI CACGTC 2 cut(s) 22, 509
BtsCI GGATG 2 cut(s) 1243, 1432
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 8 cut(s) 48, 404, 543, 858, 1009, 1222, 1303, 1725
BveI ACCTGC 1 cut(s) 1452
Cac8I GCNNGC 4 cut(s) 35, 39, 1122, 1372
CciI TCATGA 1 cut(s) 922
CfoI GCGC 2 cut(s) 1644, 1704
Cfr13I GGNCC 4 cut(s) 52, 1211, 1325, 1340
CseI GACGC 2 cut(s) 92, 107
Csp6I GTAC 2 cut(s) 1402, 1607
CspCI CAANNNNNGTGG 2 cut(s) 1318, 1353
CviQI GTAC 2 cut(s) 1402, 1607
DdeI CTNAG 3 cut(s) 532, 749, 1492
DpnI GATC 9 cut(s) 74, 186, 210, 420, 642, 957, 1092, 1418, 1482
DpnII GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
DraI TTTAAA 1 cut(s) 723
DriI GACNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 1099
Eam1104I CTCTTC 3 cut(s) 569, 632, 1665
Eam1105I GACNNNNNGTC 1 cut(s) 196
EarI CTCTTC 3 cut(s) 569, 632, 1665
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 1211, 1325
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 4 cut(s) 291, 428, 576, 723
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 1211, 1325
EcoRII CCWGG 2 cut(s) 861, 1678
EcoT22I ATGCAT 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 1704
FalI AAGNNNNNCTT 4 cut(s) 414, 446, 628, 660
FbaI TGATCA 1 cut(s) 1416
Fnu4HI GCNGC 6 cut(s) 758, 1359, 1412, 1676, 1705, 1737
FokI GGATG 2 cut(s) 1250, 1439
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 6 cut(s) 758, 1359, 1412, 1676, 1705, 1737
FspBI CTAG 4 cut(s) 251, 260, 660, 1353
GlaI GCGC 2 cut(s) 1643, 1703
GluI GCNGC 6 cut(s) 758, 1359, 1412, 1676, 1705, 1737
GsuI CTGGAG 2 cut(s) 884, 1227
HaeIII GGCC 5 cut(s) 53, 70, 1101, 1341, 1584
HgaI GACGC 2 cut(s) 92, 107
HhaI GCGC 2 cut(s) 1644, 1704
Hin1I GRCGYC 2 cut(s) 99, 1713
Hin6I GCGC 2 cut(s) 1642, 1702
HinP1I GCGC 2 cut(s) 1642, 1702
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HinfI GANTC 4 cut(s) 89, 292, 1073, 1540
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 1 cut(s) 1036
Hpy188I TCNGA 3 cut(s) 19, 457, 1480
Hpy8I GTNNAC 1 cut(s) 1036
Hpy99I CGWCG 2 cut(s) 23, 101
HpyAV CCTTC 3 cut(s) 431, 1191, 1259
HpyCH4III ACNGT 6 cut(s) 319, 547, 978, 1226, 1686, 1729
HpyCH4IV ACGT 4 cut(s) 21, 390, 508, 1713
HpyF10VI GCNNNNNNNGC 3 cut(s) 754, 1367, 1639
HpyF3I CTNAG 3 cut(s) 532, 749, 1492
HpySE526I ACGT 4 cut(s) 21, 390, 508, 1713
Hsp92I GRCGYC 2 cut(s) 99, 1713
HspAI GCGC 2 cut(s) 1642, 1702
Ksp22I TGATCA 1 cut(s) 1416
Kzo9I GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
LguI GCTCTTC 1 cut(s) 1665
LmnI GCTCC 1 cut(s) 1756
Lsp1109I GCAGC 6 cut(s) 769, 1345, 1398, 1662, 1691, 1748
LweI GCATC 3 cut(s) 1450, 1484, 1537
MaeI CTAG 4 cut(s) 251, 260, 660, 1353
MaeII ACGT 4 cut(s) 21, 390, 508, 1713
MaeIII GTNAC 4 cut(s) 188, 463, 1126, 1694
MalI GATC 9 cut(s) 74, 186, 210, 420, 642, 957, 1092, 1418, 1482
MbiI CCGCTC 2 cut(s) 120, 151
MboI GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
MboII GAAGA 7 cut(s) 244, 569, 586, 619, 903, 1475, 1682
MhlI GDGCHC 2 cut(s) 111, 1303
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 3 cut(s) 42, 393, 1486
Mph1103I ATGCAT 1 cut(s) 501
MroXI GAANNNNTTC 2 cut(s) 1146, 1269
MseI TTAA 8 cut(s) 114, 485, 650, 722, 963, 1017, 1106, 1230
MslI CAYNNNNRTG 2 cut(s) 363, 921
MspA1I CMGCKG 1 cut(s) 1205
MspCI CTTAAG 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 863, 1680
MvaI CCWGG 2 cut(s) 863, 1680
MwoI GCNNNNNNNGC 3 cut(s) 754, 1367, 1639
NdeII GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
NlaIV GGNNCC 1 cut(s) 1327
NmuCI GTSAC 2 cut(s) 188, 463
NsiI ATGCAT 1 cut(s) 501
NspI RCATGY 1 cut(s) 1702
NspV TTCGAA 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 1665
PdmI GAANNNNTTC 2 cut(s) 1146, 1269
PfeI GAWTC 3 cut(s) 292, 1073, 1540
PkrI GCNGC 6 cut(s) 759, 1360, 1413, 1677, 1706, 1738
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PpuMI RGGWCCY 2 cut(s) 1211, 1325
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 2 cut(s) 1211, 1325
Psp6I CCWGG 2 cut(s) 861, 1678
PspGI CCWGG 2 cut(s) 861, 1678
PspN4I GGNNCC 1 cut(s) 1327
PspPI GGNCC 4 cut(s) 52, 1211, 1325, 1340
PspPPI RGGWCCY 2 cut(s) 1211, 1325
PspXI VCTCGAGB 1 cut(s) 152
PsrI GAACNNNNNNTAC 2 cut(s) 1599, 1631
PstI CTGCAG 3 cut(s) 1363, 1449, 1709
PvuII CAGCTG 1 cut(s) 1205
RsaI GTAC 2 cut(s) 1403, 1608
RsaNI GTAC 2 cut(s) 1402, 1607
RseI CAYNNNNRTG 2 cut(s) 363, 921
SacI GAGCTC 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 1665
SaqAI TTAA 8 cut(s) 114, 485, 650, 722, 963, 1017, 1106, 1230
SatI GCNGC 6 cut(s) 758, 1359, 1412, 1676, 1705, 1737
Sau3AI GATC 9 cut(s) 72, 184, 208, 418, 640, 955, 1090, 1416, 1480
Sau96I GGNCC 4 cut(s) 52, 1211, 1325, 1340
SbfI CCTGCAGG 1 cut(s) 1449
ScaI AGTACT 1 cut(s) 1403
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 863, 1680
SdaI CCTGCAGG 1 cut(s) 1449
SduI GDGCHC 2 cut(s) 111, 1303
SfaNI GCATC 3 cut(s) 1450, 1484, 1537
SfcI CTRYAG 3 cut(s) 1359, 1445, 1705
Sfr274I CTCGAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 572
SinI GGWCC 2 cut(s) 1211, 1325
SlaI CTCGAG 1 cut(s) 152
SmiI ATTTAAAT 1 cut(s) 723
SmiMI CAYNNNNRTG 2 cut(s) 363, 921
SmlI CTYRAG 3 cut(s) 152, 649, 1690
SmoI CTYRAG 3 cut(s) 152, 649, 1690
Sse8387I CCTGCAGG 1 cut(s) 1449
SsiI CCGC 3 cut(s) 118, 149, 1520
SspMI CTAG 4 cut(s) 251, 260, 660, 1353
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 861, 1678
SwaI ATTTAAAT 1 cut(s) 723
TaaI ACNGT 6 cut(s) 319, 547, 978, 1226, 1686, 1729
TaiI ACGT 4 cut(s) 24, 393, 511, 1716
TaqI TCGA 9 cut(s) 153, 333, 372, 478, 572, 792, 990, 1545, 1668
TatI WGTACW 2 cut(s) 1401, 1606
TfiI GAWTC 3 cut(s) 292, 1073, 1540
Tru1I TTAA 8 cut(s) 114, 485, 650, 722, 963, 1017, 1106, 1230
Tru9I TTAA 8 cut(s) 114, 485, 650, 722, 963, 1017, 1106, 1230
TscAI CASTG 8 cut(s) 48, 411, 550, 858, 1009, 1229, 1303, 1732
TseFI GTSAC 2 cut(s) 188, 463
TseI GCWGC 6 cut(s) 757, 1358, 1411, 1675, 1704, 1736
Tsp45I GTSAC 2 cut(s) 188, 463
TspDTI ATGAA 4 cut(s) 347, 939, 981, 1012
TspGWI ACGGA 3 cut(s) 587, 1083, 1439
TspRI CASTG 8 cut(s) 48, 411, 550, 858, 1009, 1229, 1303, 1732
Vha464I CTTAAG 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 1211, 1325
XapI RAATTY 4 cut(s) 425, 593, 895, 1108
XbaI TCTAGA 1 cut(s) 659
XceI RCATGY 1 cut(s) 1702
XhoI CTCGAG 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 1146, 1269
XspI CTAG 4 cut(s) 251, 260, 660, 1353
ZraI GACGTC 1 cut(s) 1714
ZrmI AGTACT 1 cut(s) 1403
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.