pycom12g21270

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000862
Physical Location & Seq
Reverse (-)
22466406 .. 22469612
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21270.13

Sequence Viewer

Length: 1548 bp
ATGGTGGTTGTATACCTCAAGAAGAATGATGGTAAGCTAGAGGAACTTGGACGAACTGAAGTCATACTGAACAATTTAAATCCGGTATGGATACAGAAAGTTTCAGTAGCTTATCAGTTCGAGACGGTGCAGAATTTGATATTCCGTGTCTATGATGTTGATACACAATACCACAATGTACCTGTAAAGACTCTGAATTTGAGGGATCAAGAGTTTCTTGGAGAAGGCAGTTGTGTCCTTTCAGAGATTGTGACTAAACAAAATCGAAGTTTGACATTGACTCTGAAAGGCAAAAACGGGCAGGGAGGTTTGAGAAACTTTGGAACACTCACCGTGCATGCCGAGGAAACTATTGCTTCTAGGAGTGCCGTAGAGATTAAATTCCGCTGTGCCCACTTGGACAACATAGATCTCTTTTCTAAAAGCGATCCCTTCTTAAGAATATCAAGAACTGTTGAGACTGGGGGTTCTATTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAACCCGACCTGGAAACCATTATGCCTGAGTATGCAACAGTTCGGAAGCAAGGACACCCCATTGGTTATTGAGTGTTTTGATTTCAATAGCAATGGCAACCACATTCTTATTGGGAAACTCCAAAAATCAGTAGCAGACCTTGAAACACTATTCAAAGAAAAAAGTGGGGTGAACTTTTTTATTCCATCTTCTCGTGCGGGTCATGAAAAGGTTTTGAAGGGTCAGCTATTTGTGGATCAATTTTGTGAGAAGGAACAATTCAGCTTTGTTGACTATATATCTAGTGGATTTGAGCTTAACTTCATGGTTGCAGTTGATTTTACGGGTTCAAATGGAGATCCTAGAAAGCCCGAGTCTTTGCACTATATTGATCCGTATGGCCGGCTGAATTCTTACCAGCAGGCTATAACAGAAGTAGGACAAGTCATTCAGTTCTACGATGCAGATAAGAGTTTTCCTGCTTGGGGCTTTGGAGGAAGGACGGCTAATGGAACTGTATCACACTGCTTCAACTTGAATGGAAATGCAAATGAATCTGAGGTTGTCGGAGTTGAAGGCATAATGGCTGCTTATGCCAGTGCTGTACGCAATGTTGCTCTGTCAGGACCGACGTTGTTTGGCCTAGTGGTTAACAGGGCTGCTGAAATTGCCGCCAGGTCCCTCTCGTCCAACAACAAGTACTACGTCTTGCTCATTATAACGGATGGAATCCTCACCGACCTTCAAGAAACAAAAGATGCTCTGGTGAGGGCATCTAATCTTCCTCTATCAATCCTCATCGTTGGAGTCGGTAATGCAGATTTCAAACAAATGGAGATCCTTGATGCTGACAACGGGCAACGATTAGAAAGCTCTACAGGTCGCATAGCTACGCGTGACATTGTCCAGTTTGTTCCAATGAGAGATGTGCAAAGTGGGCAGATTTCTGTTGTTCAAGGTCTTTTGGAAGAGCTGCCCGGACAGTTTTTGAGTTACATGCGTTCCATGGACATCAAGCCGCTCATCTTCAATGCCGCTGCCCAAGTATCACCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

516

Amino Acids

56.88

Weight (kDa)

5.46

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1208
AccBSI CCGCTC 1 cut(s) 1510
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 1384
AciI CCGC 5 cut(s) 385, 707, 1161, 1508, 1524
AclWI GGATC 6 cut(s) 213, 422, 753, 842, 875, 1321
AcoI YGGCCR 1 cut(s) 889
AcsI RAATTY 4 cut(s) 133, 196, 380, 898
AcuI CTGAAG 2 cut(s) 78, 510
AfaI GTAC 3 cut(s) 180, 1095, 1190
AfiI CCNNNNNNNGG 1 cut(s) 974
AflII CTTAAG 1 cut(s) 436
AflIII ACRYGT 1 cut(s) 1382
AjnI CCWGG 2 cut(s) 518, 1163
AluBI AGCT 8 cut(s) 37, 110, 736, 774, 805, 1362, 1379, 1462
AluI AGCT 8 cut(s) 37, 110, 736, 774, 805, 1362, 1379, 1462
Alw26I GTCTC 2 cut(s) 116, 452
AlwI GGATC 6 cut(s) 213, 422, 753, 842, 875, 1321
Ama87I CYCGRG 1 cut(s) 860
AoxI GGCC 2 cut(s) 889, 1129
ApeKI GCWGC 4 cut(s) 1076, 1148, 1462, 1526
ApoI RAATTY 4 cut(s) 133, 196, 380, 898
ArsI GACNNNNNNTTYG 2 cut(s) 181, 213
AspS9I GGNCC 2 cut(s) 1115, 1167
AsuC2I CCSGG 1 cut(s) 1467
AsuHPI GGTGA 5 cut(s) 322, 691, 1216, 1267, 1530
AvaI CYCGRG 1 cut(s) 860
AvaII GGWCC 2 cut(s) 1115, 1167
BaeGI GKGCMC 1 cut(s) 394
BauI CACGAG 1 cut(s) 702
BbvI GCAGC 4 cut(s) 1063, 1135, 1449, 1513
BccI CCATC 3 cut(s) 23, 703, 1208
BceAI ACGGC 2 cut(s) 353, 1008
BciT130I CCWGG 2 cut(s) 520, 1165
BciVI GTATCC 1 cut(s) 84
BcnI CCSGG 1 cut(s) 1467
BcoDI GTCTC 2 cut(s) 116, 452
BfaI CTAG 5 cut(s) 38, 360, 792, 852, 1133
BfmI CTRYAG 1 cut(s) 1365
BfrI CTTAAG 1 cut(s) 436
BfuI GTATCC 1 cut(s) 84
BglII AGATCT 1 cut(s) 409
BisI GCNGC 7 cut(s) 1077, 1149, 1161, 1463, 1508, 1524, 1527
BlsI GCNGC 7 cut(s) 1078, 1150, 1162, 1464, 1509, 1525, 1528
BmcAI AGTACT 1 cut(s) 1190
Bme1390I CCNGG 3 cut(s) 520, 1165, 1467
Bme18I GGWCC 2 cut(s) 1115, 1167
BmeT110I CYCGRG 1 cut(s) 860
BmgT120I GGNCC 2 cut(s) 1115, 1167
BmiI GGNNCC 1 cut(s) 1169
BmrFI CCNGG 3 cut(s) 520, 1165, 1467
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 4 cut(s) 940, 1237, 1271, 1324
BmuI ACTGGG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 1467
BsaJI CCNNGG 2 cut(s) 342, 1494
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 974
Bse118I RCCGGY 1 cut(s) 891
Bse1I ACTGG 3 cut(s) 466, 1086, 1396
Bse3DI GCAATG 2 cut(s) 607, 1105
BseBI CCWGG 2 cut(s) 520, 1165
BseDI CCNNGG 2 cut(s) 342, 1494
BseGI GGATG 1 cut(s) 1219
BseLI CCNNNNNNNGG 1 cut(s) 974
BseMI GCAATG 2 cut(s) 607, 1105
BseMII CTCAG 2 cut(s) 527, 1038
BseNI ACTGG 3 cut(s) 466, 1086, 1396
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 4 cut(s) 1063, 1135, 1449, 1513
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 1384
BshFI GGCC 2 cut(s) 891, 1131
BsiHKCI CYCGRG 1 cut(s) 860
BsiSI CCGG 3 cut(s) 83, 892, 1467
BslFI GGGAC 1 cut(s) 1153
BslI CCNNNNNNNGG 1 cut(s) 974
BsmAI GTCTC 2 cut(s) 116, 452
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 1153
BsnI GGCC 2 cut(s) 891, 1131
BsoBI CYCGRG 1 cut(s) 860
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
Bsp19I CCATGG 1 cut(s) 1494
BspACI CCGC 5 cut(s) 385, 707, 1161, 1508, 1524
BspANI GGCC 2 cut(s) 891, 1131
BspCNI CTCAG 2 cut(s) 528, 1039
BspFNI CGCG 1 cut(s) 1384
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 1169
BspPI GGATC 6 cut(s) 213, 422, 753, 842, 875, 1321
BspQI GCTCTTC 1 cut(s) 1452
BspTI CTTAAG 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 1510
BsrDI GCAATG 2 cut(s) 607, 1105
BsrFI RCCGGY 1 cut(s) 891
BsrI ACTGG 3 cut(s) 466, 1086, 1396
BssAI RCCGGY 1 cut(s) 891
BssECI CCNNGG 2 cut(s) 342, 1494
BssMI GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
BssNAI GTATAC 1 cut(s) 13
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 1494
Bst1107I GTATAC 1 cut(s) 13
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 520, 1165
Bst4CI ACNGT 6 cut(s) 127, 334, 454, 549, 1006, 1473
Bst6I CTCTTC 1 cut(s) 1452
BstAFI CTTAAG 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 339, 893, 912
BstDEI CTNAG 2 cut(s) 536, 1047
BstDSI CCRYGG 1 cut(s) 1494
BstF5I GGATG 1 cut(s) 1219
BstFNI CGCG 1 cut(s) 1384
BstKTI GATC 7 cut(s) 208, 412, 430, 748, 850, 883, 1329
BstMAI GTCTC 2 cut(s) 116, 452
BstMBI GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
BstMWI GCNNNNNNNGC 3 cut(s) 1082, 1157, 1426
BstNI CCWGG 2 cut(s) 520, 1165
BstNSI RCATGY 2 cut(s) 341, 1489
BstSCI CCNGG 3 cut(s) 518, 1163, 1465
BstSFI CTRYAG 1 cut(s) 1365
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 1384
BstV1I GCAGC 4 cut(s) 1063, 1135, 1449, 1513
BstX2I RGATCY 3 cut(s) 409, 847, 1326
BstYI RGATCY 3 cut(s) 409, 847, 1326
BstZ17I GTATAC 1 cut(s) 13
BsuI GTATCC 1 cut(s) 84
BsuRI GGCC 2 cut(s) 891, 1131
BtgI CCRYGG 1 cut(s) 1494
BtsCI GGATG 1 cut(s) 1219
BtsI GCAGTG 1 cut(s) 1012
BtsIMutI CAGTG 2 cut(s) 1012, 1093
Cac8I GCNNGC 3 cut(s) 339, 893, 912
CciI TCATGA 1 cut(s) 712
Cfr10I RCCGGY 1 cut(s) 891
Cfr13I GGNCC 2 cut(s) 1115, 1167
Csp6I GTAC 3 cut(s) 179, 1094, 1189
CviAII CATG 5 cut(s) 338, 713, 814, 1486, 1495
CviQI GTAC 3 cut(s) 179, 1094, 1189
DdeI CTNAG 2 cut(s) 536, 1047
DpnI GATC 7 cut(s) 207, 411, 429, 747, 849, 882, 1328
DpnII GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
DraI TTTAAA 2 cut(s) 78, 510
EaeI YGGCCR 1 cut(s) 889
Eam1104I CTCTTC 1 cut(s) 1452
EarI CTCTTC 1 cut(s) 1452
Eco130I CCWWGG 1 cut(s) 1494
Eco47I GGWCC 2 cut(s) 1115, 1167
Eco57I CTGAAG 2 cut(s) 78, 510
Eco88I CYCGRG 1 cut(s) 860
EcoO109I RGGNCCY 1 cut(s) 1167
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 518, 1163
EcoT14I CCWWGG 1 cut(s) 1494
ErhI CCWWGG 1 cut(s) 1494
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 5 cut(s) 341, 716, 817, 1489, 1498
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 1153
FatI CATG 5 cut(s) 337, 712, 813, 1485, 1494
FauI CCCGC 1 cut(s) 700
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 7 cut(s) 1077, 1149, 1161, 1463, 1508, 1524, 1527
FokI GGATG 1 cut(s) 1226
Fsp4HI GCNGC 7 cut(s) 1077, 1149, 1161, 1463, 1508, 1524, 1527
FspBI CTAG 5 cut(s) 38, 360, 792, 852, 1133
GluI GCNGC 7 cut(s) 1077, 1149, 1161, 1463, 1508, 1524, 1527
HaeIII GGCC 2 cut(s) 891, 1131
HapII CCGG 3 cut(s) 83, 892, 1467
Hin1II CATG 5 cut(s) 341, 716, 817, 1489, 1498
HincII GTYRAC 2 cut(s) 781, 1141
HindII GTYRAC 2 cut(s) 781, 1141
HinfI GANTC 6 cut(s) 190, 280, 863, 1043, 1218, 1296
HpaI GTTAAC 1 cut(s) 1141
HpaII CCGG 3 cut(s) 83, 892, 1467
HphI GGTGA 5 cut(s) 322, 691, 1216, 1267, 1530
Hpy166II GTNNAC 4 cut(s) 13, 682, 781, 1141
Hpy188I TCNGA 6 cut(s) 195, 244, 285, 554, 1048, 1058
Hpy188III TCNNGA 7 cut(s) 19, 121, 209, 447, 713, 1113, 1235
Hpy8I GTNNAC 4 cut(s) 13, 682, 781, 1141
Hpy99I CGWCG 1 cut(s) 1123
HpyAV CCTTC 7 cut(s) 218, 442, 721, 754, 981, 1058, 1241
HpyCH4III ACNGT 6 cut(s) 127, 334, 454, 549, 1006, 1473
HpyCH4IV ACGT 2 cut(s) 1121, 1194
HpyF10VI GCNNNNNNNGC 3 cut(s) 1082, 1157, 1426
HpyF3I CTNAG 2 cut(s) 536, 1047
HpySE526I ACGT 2 cut(s) 1121, 1194
Hsp92II CATG 5 cut(s) 341, 716, 817, 1489, 1498
KroI GCCGGC 1 cut(s) 891
KroNI GCCGGC 1 cut(s) 893
KspAI GTTAAC 1 cut(s) 1141
Kzo9I GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
LguI GCTCTTC 1 cut(s) 1452
Lsp1109I GCAGC 4 cut(s) 1063, 1135, 1449, 1513
LweI GCATC 4 cut(s) 940, 1237, 1271, 1324
MaeI CTAG 5 cut(s) 38, 360, 792, 852, 1133
MaeII ACGT 2 cut(s) 1121, 1194
MaeIII GTNAC 3 cut(s) 250, 1385, 1481
MalI GATC 7 cut(s) 207, 411, 429, 747, 849, 882, 1328
MbiI CCGCTC 1 cut(s) 1510
MboI GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
MboII GAAGA 5 cut(s) 34, 690, 1262, 1469, 1507
MflI RGATCY 3 cut(s) 409, 847, 1326
MhlI GDGCHC 1 cut(s) 394
MluI ACGCGT 1 cut(s) 1382
MlyI GAGTC 4 cut(s) 184, 274, 872, 1305
MmeI TCCRAC 3 cut(s) 1036, 1203, 1273
MroNI GCCGGC 1 cut(s) 891
MseI TTAA 6 cut(s) 77, 378, 437, 509, 807, 1140
MspA1I CMGCKG 2 cut(s) 387, 1526
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 3 cut(s) 83, 892, 1467
MspR9I CCNGG 3 cut(s) 520, 1165, 1467
MvaI CCWGG 2 cut(s) 520, 1165
MvnI CGCG 1 cut(s) 1384
MwoI GCNNNNNNNGC 3 cut(s) 1082, 1157, 1426
NaeI GCCGGC 1 cut(s) 893
NciI CCSGG 1 cut(s) 1467
NcoI CCATGG 1 cut(s) 1494
NdeII GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
NgoMIV GCCGGC 1 cut(s) 891
NlaIII CATG 5 cut(s) 341, 716, 817, 1489, 1498
NlaIV GGNNCC 1 cut(s) 1169
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 250, 1385
NspI RCATGY 2 cut(s) 341, 1489
PaeI GCATGC 1 cut(s) 341
PagI TCATGA 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 1452
PdiI GCCGGC 1 cut(s) 893
PfeI GAWTC 2 cut(s) 1043, 1218
PflFI GACNNNGTC 1 cut(s) 1391
PkrI GCNGC 7 cut(s) 1078, 1150, 1162, 1464, 1509, 1525, 1528
PleI GAGTC 4 cut(s) 184, 274, 871, 1304
PpsI GAGTC 4 cut(s) 184, 274, 871, 1304
PpuMI RGGWCCY 1 cut(s) 1167
PsiI TTATAA 1 cut(s) 1208
Psp5II RGGWCCY 1 cut(s) 1167
Psp6I CCWGG 2 cut(s) 518, 1163
PspGI CCWGG 2 cut(s) 518, 1163
PspN4I GGNNCC 1 cut(s) 1169
PspPI GGNCC 2 cut(s) 1115, 1167
PspPPI RGGWCCY 1 cut(s) 1167
PsuI RGATCY 3 cut(s) 409, 847, 1326
PsyI GACNNNGTC 1 cut(s) 1391
RsaI GTAC 3 cut(s) 180, 1095, 1190
RsaNI GTAC 3 cut(s) 179, 1094, 1189
SapI GCTCTTC 1 cut(s) 1452
SaqAI TTAA 6 cut(s) 77, 378, 437, 509, 807, 1140
SatI GCNGC 7 cut(s) 1077, 1149, 1161, 1463, 1508, 1524, 1527
Sau3AI GATC 7 cut(s) 205, 409, 427, 745, 847, 880, 1326
Sau96I GGNCC 2 cut(s) 1115, 1167
ScaI AGTACT 1 cut(s) 1190
SchI GAGTC 4 cut(s) 184, 274, 872, 1305
ScrFI CCNGG 3 cut(s) 520, 1165, 1467
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 4 cut(s) 940, 1237, 1271, 1324
SfcI CTRYAG 1 cut(s) 1365
SinI GGWCC 2 cut(s) 1115, 1167
SmiI ATTTAAAT 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 17, 436
SmoI CTYRAG 2 cut(s) 17, 436
SphI GCATGC 1 cut(s) 341
SsiI CCGC 5 cut(s) 385, 707, 1161, 1508, 1524
SspMI CTAG 5 cut(s) 38, 360, 792, 852, 1133
StyD4I CCNGG 3 cut(s) 518, 1163, 1465
StyI CCWWGG 1 cut(s) 1494
SwaI ATTTAAAT 1 cut(s) 78
TaaI ACNGT 6 cut(s) 127, 334, 454, 549, 1006, 1473
TaiI ACGT 2 cut(s) 1124, 1197
TaqI TCGA 2 cut(s) 120, 265
TaqII GACCGA 1 cut(s) 1132
TatI WGTACW 1 cut(s) 1188
TauI GCSGC 3 cut(s) 1163, 1510, 1526
TfiI GAWTC 2 cut(s) 1043, 1218
Tru1I TTAA 6 cut(s) 77, 378, 437, 509, 807, 1140
Tru9I TTAA 6 cut(s) 77, 378, 437, 509, 807, 1140
TscAI CASTG 2 cut(s) 1019, 1093
TseFI GTSAC 2 cut(s) 250, 1385
TseI GCWGC 4 cut(s) 1076, 1148, 1462, 1526
Tsp45I GTSAC 2 cut(s) 250, 1385
TspDTI ATGAA 3 cut(s) 729, 802, 1056
TspGWI ACGGA 3 cut(s) 134, 873, 1226
TspRI CASTG 2 cut(s) 1019, 1093
Tth111I GACNNNGTC 1 cut(s) 1391
Vha464I CTTAAG 1 cut(s) 436
VpaK11BI GGWCC 2 cut(s) 1115, 1167
XapI RAATTY 4 cut(s) 133, 196, 380, 898
XceI RCATGY 2 cut(s) 341, 1489
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 5 cut(s) 38, 360, 792, 852, 1133
ZrmI AGTACT 1 cut(s) 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.