Rorug02G0648500

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000862
Physical Location & Seq
Forward (+)
75783362 .. 75786731
3370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0648500.1

Sequence Viewer

Length: 1251 bp
ATGGGCTATTGCCCCTGTTTCGGTTTGAGGAAGGGCAAGAAGCTCAAGCGCGGCGAAGACGACGACGTTCACAAAACCCCTTCTTCCGACAATGCCACGCCTCAATTGTCTTCTGCTCAAGAAGATGGAACTAATGATCCTACTAAGGCACAGACATTCACCTTTCGTGAACTTGCAACTGCAACCAAAAATTTTAGGGCGGAATGCTTTATTGGGGAAGGTGGATTTGGGCCTGTATACAAAGGGAAATTGAGTTCTGGTCGAGTTGTAGCTGTAAAGAAACTAGATCAGACAGGTCTTCAAGGAGACAAGGAGTTTCTGGTGGAGGTTCTAATGCTTTCTCTTCTGCGTCACCCAAACCTTGTAAGTTTAATTGGTTACTGTGCTGAAGGGGAGCAGCGCCTGCTTGTCTATGAATACATGCCCTTAGGGTCCTTGGCAGACCATCTCCATGATCTTAGACCTGATAAAGAACCACTTGATTGGAACACAAGGATGATGATAGCTGCTGGTGCAGCCAAAGGGTTGGAATATCTTCACCATGATGCTGAACCACCTGTCATATACAGGGATGTAAAATCAGCCAACATTTTGTTGGGGGAGGATTTTTTCCCGAAACTTTCTGATTTTGGGCTTGCAAAGTTTGGTCCTACTGGAGATAGATCGCATGTCTCCACCAGGGTCATGGGAACTCATGGTTACTGTGCTCCTGAGTATGCTAAAAGTGGAAAGTTGACAGCAAAATCGGATATCTACAGTTTTGGGGTAGTCTTGCTGGAGTTGCTTACAGGACATAAGGCCGTTGATAGTACTCTTGGTCGTGAAAAACAGCTTGTTGAGTGGGCACGTCCTTTTATGAAAGATCCCAAGAAGCTTACCATGCAATTTGCAGATCCATATCTTAGAGGGCAGTTTCCAGTACATATCTTGAGAAACGCATTAGAAGTGGCTTCCATGTGTCTAAGAGAGGATGCTTCTGCCCGACCGACCATGAGGGATGTGGTACTTGCCTTGAATTACTTGACAACCCATCCATATAATGAACCAAAGGTGTTGAGCATCAGCACTGAGCGGGTTGAAGGAATAAAGAGTTTGAATGCTTCAGGGAATAACTCTCCTAATGAAACGACATGTATGTTAAACAAGAACTTGGACAGAGAAAAAGCTGTTGCAGAGGCCAAGATGTGGGGAGAGACTTGGAGAGACAAGCGTGGTGCACAAAATAGTCGTCATGATGCCTTTAGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

416

Amino Acids

46.26

Weight (kDa)

8.05

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 70 - 335 5e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 70 - 336 4.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1185
AccBSI CCGCTC 1 cut(s) 1072
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 51, 200, 1072
AclWI GGATC 3 cut(s) 131, 857, 887
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 2 cut(s) 408, 1086
AfaI GTAC 3 cut(s) 811, 921, 1005
AfiI CCNNNNNNNGG 2 cut(s) 20, 1185
AflIII ACRYGT 1 cut(s) 1130
AgsI TTSAA 4 cut(s) 302, 1015, 1079, 1096
AjiI CACGTC 1 cut(s) 848
AjnI CCWGG 1 cut(s) 677
AjuI GAANNNNNNNTTGG 4 cut(s) 195, 210, 227, 242
AloI GAACNNNNNNTCC 4 cut(s) 121, 153, 238, 270
AluBI AGCT 6 cut(s) 43, 272, 506, 832, 874, 1166
AluI AGCT 6 cut(s) 43, 272, 506, 832, 874, 1166
Alw21I GWGCWC 2 cut(s) 709, 1219
Alw26I GTCTC 4 cut(s) 300, 676, 1187, 1197
Alw44I GTGCAC 1 cut(s) 1215
AlwI GGATC 3 cut(s) 131, 857, 887
AlwNI CAGNNNCTG 1 cut(s) 403
AoxI GGCC 3 cut(s) 230, 798, 1176
ApaLI GTGCAC 1 cut(s) 1215
ApeKI GCWGC 3 cut(s) 397, 506, 515
ApoI RAATTY 1 cut(s) 190
ArsI GACNNNNNNTTYG 2 cut(s) 1213, 1245
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 2 cut(s) 51, 402
AspS9I GGNCC 3 cut(s) 230, 432, 647
AsuHPI GGTGA 3 cut(s) 151, 344, 530
AvaII GGWCC 2 cut(s) 432, 647
AxyI CCTNAGG 1 cut(s) 427
BaeGI GKGCMC 2 cut(s) 847, 1219
BbsI GAAGAC 3 cut(s) 63, 102, 290
Bbv12I GWGCWC 2 cut(s) 709, 1219
BbvI GCAGC 3 cut(s) 409, 493, 527
BccI CCATC 3 cut(s) 119, 453, 1038
BceAI ACGGC 1 cut(s) 785
BciT130I CCWGG 1 cut(s) 679
BcoDI GTCTC 4 cut(s) 300, 676, 1187, 1197
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 754
BfoI RGCGCY 1 cut(s) 403
BisI GCNGC 4 cut(s) 52, 398, 507, 516
BlsI GCNGC 4 cut(s) 53, 399, 508, 517
BmcAI AGTACT 1 cut(s) 811
Bme1390I CCNGG 1 cut(s) 679
Bme18I GGWCC 2 cut(s) 432, 647
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 3 cut(s) 230, 432, 647
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 679
BmsI GCATC 4 cut(s) 535, 961, 1068, 1225
BpiI GAAGAC 3 cut(s) 63, 102, 290
BpmI CTGGAG 2 cut(s) 675, 797
BpuEI CTTGAG 3 cut(s) 29, 102, 949
BsaBI GATNNNNATC 1 cut(s) 897
BsaJI CCNNGG 2 cut(s) 435, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 1185
Bse1I ACTGG 2 cut(s) 658, 917
Bse21I CCTNAGG 1 cut(s) 427
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 1 cut(s) 679
BseDI CCNNGG 2 cut(s) 435, 678
BseGI GGATG 5 cut(s) 501, 577, 976, 1003, 1030
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 2 cut(s) 20, 1185
BseMII CTCAG 2 cut(s) 702, 1059
BseNI ACTGG 2 cut(s) 658, 917
BseSI GKGCMC 2 cut(s) 847, 1219
BseXI GCAGC 3 cut(s) 409, 493, 527
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 986
BshFI GGCC 3 cut(s) 232, 800, 1178
BsiEI CGRYCG 1 cut(s) 986
BsiHKAI GWGCWC 2 cut(s) 709, 1219
BslI CCNNNNNNNGG 2 cut(s) 20, 1185
BsmAI GTCTC 4 cut(s) 300, 676, 1187, 1197
BsmI GAATGC 2 cut(s) 209, 1102
BsnI GGCC 3 cut(s) 232, 800, 1178
Bsp1286I GDGCHC 3 cut(s) 709, 847, 1219
Bsp143I GATC 6 cut(s) 136, 286, 454, 662, 862, 892
BspACI CCGC 3 cut(s) 51, 200, 1072
BspANI GGCC 3 cut(s) 232, 800, 1178
BspCNI CTCAG 2 cut(s) 703, 1060
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 1 cut(s) 433
BspPI GGATC 3 cut(s) 131, 857, 887
BsrBI CCGCTC 1 cut(s) 1072
BsrI ACTGG 2 cut(s) 658, 917
BssECI CCNNGG 2 cut(s) 435, 678
BssMI GATC 6 cut(s) 136, 286, 454, 662, 862, 892
BssNAI GTATAC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 679
Bst4CI ACNGT 3 cut(s) 383, 704, 758
Bst6I CTCTTC 1 cut(s) 348
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 404, 636
BstDEI CTNAG 7 cut(s) 144, 427, 458, 711, 902, 962, 1068
BstF5I GGATG 5 cut(s) 501, 577, 976, 1003, 1030
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 2 cut(s) 51, 402
BstKTI GATC 6 cut(s) 139, 289, 457, 665, 865, 895
BstMAI GTCTC 4 cut(s) 300, 676, 1187, 1197
BstMBI GATC 6 cut(s) 136, 286, 454, 662, 862, 892
BstMCI CGRYCG 1 cut(s) 986
BstMWI GCNNNNNNNGC 5 cut(s) 403, 512, 515, 781, 880
BstNI CCWGG 1 cut(s) 679
BstNSI RCATGY 3 cut(s) 424, 671, 1134
BstSCI CCNGG 1 cut(s) 677
BstSFI CTRYAG 1 cut(s) 754
BstSLI GKGCMC 2 cut(s) 847, 1219
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 3 cut(s) 409, 493, 527
BstV2I GAAGAC 3 cut(s) 63, 102, 290
BstX2I RGATCY 2 cut(s) 862, 892
BstXI CCANNNNNNTGG 3 cut(s) 483, 526, 685
BstYI RGATCY 2 cut(s) 862, 892
BstZ17I GTATAC 1 cut(s) 238
Bsu36I CCTNAGG 1 cut(s) 427
BsuRI GGCC 3 cut(s) 232, 800, 1178
BtrI CACGTC 1 cut(s) 848
BtsCI GGATG 5 cut(s) 501, 577, 976, 1003, 1030
BtsIMutI CAGTG 1 cut(s) 1065
Cac8I GCNNGC 2 cut(s) 404, 636
CaiI CAGNNNCTG 1 cut(s) 403
CciI TCATGA 1 cut(s) 1231
CfoI GCGC 2 cut(s) 51, 402
Cfr13I GGNCC 3 cut(s) 230, 432, 647
CseI GACGC 1 cut(s) 338
Csp6I GTAC 3 cut(s) 810, 920, 1004
CviQI GTAC 3 cut(s) 810, 920, 1004
DdeI CTNAG 7 cut(s) 144, 427, 458, 711, 902, 962, 1068
DpnI GATC 6 cut(s) 138, 288, 456, 664, 864, 894
DpnII GATC 6 cut(s) 136, 286, 454, 662, 862, 892
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 435
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 432, 647
Eco57I CTGAAG 2 cut(s) 408, 1086
Eco81I CCTNAGG 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 677
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FauI CCCGC 1 cut(s) 1065
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 52, 398, 507, 516
FokI GGATG 5 cut(s) 508, 584, 983, 1010, 1017
Fsp4HI GCNGC 4 cut(s) 52, 398, 507, 516
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 50, 401
GluI GCNGC 4 cut(s) 52, 398, 507, 516
GsuI CTGGAG 2 cut(s) 675, 797
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 3 cut(s) 232, 800, 1178
HgaI GACGC 1 cut(s) 338
HhaI GCGC 2 cut(s) 51, 402
Hin6I GCGC 2 cut(s) 49, 400
HinP1I GCGC 2 cut(s) 49, 400
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HindIII AAGCTT 1 cut(s) 872
HphI GGTGA 3 cut(s) 151, 344, 530
Hpy166II GTNNAC 5 cut(s) 70, 170, 238, 735, 1217
Hpy188I TCNGA 4 cut(s) 88, 291, 625, 748
Hpy188III TCNNGA 7 cut(s) 119, 167, 613, 710, 821, 928, 1232
Hpy8I GTNNAC 5 cut(s) 70, 170, 238, 735, 1217
Hpy99I CGWCG 2 cut(s) 65, 68
HpyAV CCTTC 5 cut(s) 25, 90, 212, 383, 1073
HpyCH4III ACNGT 3 cut(s) 383, 704, 758
HpyCH4IV ACGT 2 cut(s) 66, 847
HpyCH4V TGCA 8 cut(s) 176, 182, 515, 638, 883, 890, 1172, 1217
HpyF10VI GCNNNNNNNGC 5 cut(s) 403, 512, 515, 781, 880
HpyF3I CTNAG 7 cut(s) 144, 427, 458, 711, 902, 962, 1068
HpySE526I ACGT 2 cut(s) 66, 847
HspAI GCGC 2 cut(s) 49, 400
Kzo9I GATC 6 cut(s) 136, 286, 454, 662, 862, 892
LmnI GCTCC 2 cut(s) 394, 712
Lsp1109I GCAGC 3 cut(s) 409, 493, 527
LweI GCATC 4 cut(s) 535, 961, 1068, 1225
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 66, 847
MaeIII GTNAC 3 cut(s) 350, 377, 698
MalI GATC 6 cut(s) 138, 288, 456, 664, 864, 894
MbiI CCGCTC 1 cut(s) 1072
MboI GATC 6 cut(s) 136, 286, 454, 662, 862, 892
MboII GAAGA 7 cut(s) 68, 75, 102, 134, 290, 335, 527
MfeI CAATTG 1 cut(s) 104
MflI RGATCY 2 cut(s) 862, 892
MhlI GDGCHC 3 cut(s) 709, 847, 1219
MluCI AATT 6 cut(s) 104, 190, 248, 372, 884, 1015
MmeI TCCRAC 2 cut(s) 111, 507
MnlI CCTC 8 cut(s) 21, 111, 319, 595, 899, 961, 987, 1168
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 2 cut(s) 371, 1139
MslI CAYNNNNRTG 3 cut(s) 450, 494, 543
MspR9I CCNGG 1 cut(s) 679
MunI CAATTG 1 cut(s) 104
Mva1269I GAATGC 2 cut(s) 209, 1102
MvaI CCWGG 1 cut(s) 679
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 403, 512, 515, 781, 880
NdeII GATC 6 cut(s) 136, 286, 454, 662, 862, 892
NlaIV GGNNCC 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 3 cut(s) 424, 671, 1134
PagI TCATGA 1 cut(s) 1231
PciI ACATGT 1 cut(s) 1130
PctI GAATGC 2 cut(s) 209, 1102
PdmI GAANNNNTTC 1 cut(s) 534
PflMI CCANNNNNTGG 1 cut(s) 1185
PkrI GCNGC 4 cut(s) 53, 399, 508, 517
PpuMI RGGWCCY 1 cut(s) 432
PscI ACATGT 1 cut(s) 1130
Psp5II RGGWCCY 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 677
PspGI CCWGG 1 cut(s) 677
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 3 cut(s) 230, 432, 647
PspPPI RGGWCCY 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 862, 892
RsaI GTAC 3 cut(s) 811, 921, 1005
RsaNI GTAC 3 cut(s) 810, 920, 1004
RseI CAYNNNNRTG 3 cut(s) 450, 494, 543
SaqAI TTAA 2 cut(s) 371, 1139
SatI GCNGC 4 cut(s) 52, 398, 507, 516
Sau3AI GATC 6 cut(s) 136, 286, 454, 662, 862, 892
Sau96I GGNCC 3 cut(s) 230, 432, 647
ScaI AGTACT 1 cut(s) 811
ScrFI CCNGG 1 cut(s) 679
SduI GDGCHC 3 cut(s) 709, 847, 1219
SfaNI GCATC 4 cut(s) 535, 961, 1068, 1225
SfcI CTRYAG 1 cut(s) 754
SinI GGWCC 2 cut(s) 432, 647
SmiMI CAYNNNNRTG 3 cut(s) 450, 494, 543
SmlI CTYRAG 3 cut(s) 44, 117, 928
SmoI CTYRAG 3 cut(s) 44, 117, 928
Sse9I AATT 6 cut(s) 104, 190, 248, 372, 884, 1015
SsiI CCGC 3 cut(s) 51, 200, 1072
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 1 cut(s) 677
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 3 cut(s) 383, 704, 758
TaiI ACGT 2 cut(s) 69, 850
TaqI TCGA 1 cut(s) 262
TaqII GACCGA 1 cut(s) 1000
TasI AATT 6 cut(s) 104, 190, 248, 372, 884, 1015
TatI WGTACW 2 cut(s) 809, 919
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 2 cut(s) 371, 1139
Tru9I TTAA 2 cut(s) 371, 1139
TscAI CASTG 1 cut(s) 1072
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 3 cut(s) 397, 506, 515
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 429, 872, 1056, 1137
TspRI CASTG 1 cut(s) 1072
Van91I CCANNNNNTGG 1 cut(s) 1185
VneI GTGCAC 1 cut(s) 1215
VpaK11BI GGWCC 2 cut(s) 432, 647
XapI RAATTY 1 cut(s) 190
XceI RCATGY 3 cut(s) 424, 671, 1134
XcmI CCANNNNNNNNNTGG 3 cut(s) 592, 682, 997
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.