Rh3BG052900

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000862
Physical Location & Seq
Forward (+)
3623340 .. 3629430
6091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG052900.1

Sequence Viewer

Length: 1449 bp
ATGGGAGGGTGTTTTTCCGACGTGAAAGGTGGCAAGCAGGCCGTGGGAGGTGGGGCCCATCATCTTCAACAACAGAGGCCCATCAACAGGGACGCTGACACAAACGACGCCGTTGAGCTCTTTAACCGCTCCCGTGGCGTTTTTCCACTCTACTCTCCGCTCGAGTTATCGCTGTCAGCCACTAACTTGCTTGATCGTGATATTCTGTCAAAGAGTGATCCCATGGTTGTGGCTTATGTGAAGAAAGGAGATGGTAAGCTAGAGGAACTAGGGAGAACTGAAGTAATACTGAATAGTTTGAATCCAGCGTGGATTGAGAAAATTACAGTTTCATATCAGTTTGAGGTTGTGCAGCCATTGATATTCCATGTCTATGATGTCGATACAAAATATCACAATGTAAATGTAAAGACACTGAAGTTGGAGGATCAAGAATTTCTTGGGGAAGGCAATTGCGTTCTTTCAGAGATAGTGACCAAACAAAGTCGAAGTTTAACCTTTAATCTCCATAACATAAATGGGCGTGGAGGTTTGAGAAACTTTGGAACACTCACTGTCCATGCTGAAGAAACTATGGCTTCAAGGAGTGTTGTTGAGATAAAATTCCATTGTTCCCAATTGGAAAACAAAGACCTCTTCTCTAAAAGTGATCCTTTCTTAAGAGTATCTAGAATTGTTGAGAGTGGAGGTTCTGTTCCAATTTGCAAGACTGAAGTGGTGGATAACAATTTAAATCCAATCTGGAAACCACTATCCCTGAGTATGCAGCAGTTTGGAAGCAAGGATAATCCATTAGTCATAGAGTGCTTTGATTTCAACAGCAATGGCAATCATGTTCTTATTGGGAAACTTCAAAAATCAGTGGCAGACCTGGAGAGATTGTACAAAGAAAGAAGTGGGGTGAATTTCGTCATTCCATCTTCTCATGGTCATGAAAAGGTTTCAAAGGGTCAGTTGTTTGTGGATCAATTTAATGAGAAGCAACAGTTCAGTTTTCTCGATTATATTTCCAGTGGATTTGAGCTTAACTTCATGGTTGCGGTTGACTTTACAGCTTCAAATGGAAACCCTCAACAACATGATTCATTGCACTACAATGATCCGTATGGCCAGTTGAATTCTTACCAGCAGGCAATTACGGAAGTTGGGGAAGTCATTCAGTTTTATGATGCTGATAAACGGTTTCCTGCTTGGGGCTTTGGAGGAAGGACAGCTGGAGGGCCTGTATCGCACTGTTTTAACTTGGATGGAACTGCAACTGGCTTTGAGGTCGAAGGAGTTCAAGGCATAATGACTGCTTATGCCAGTGCCCTACACAATGTTTCTCTATCAGGACCCACTTTGTTTGGCCCAGTGGTTACTAGGGCTGCAGAGATTGCTGGCAATTCTCTATTCCACAGCAACAATAAGTACTATGTGCTGCTGATCATAACGGCAAGAGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

482

Amino Acids

53.33

Weight (kDa)

5.77

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 58 - 168 1.2e-12 C2 domain
C2 PF00168 203 - 292 1.4e-12 C2 domain
Copine PF07002 362 - 478 1.7e-36 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 129, 160
AciI CCGC 3 cut(s) 127, 158, 1040
AclWI GGATC 5 cut(s) 212, 435, 644, 972, 1094
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 4 cut(s) 434, 602, 904, 1117
AcuI CTGAAG 4 cut(s) 300, 437, 585, 732
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 2 cut(s) 884, 1412
AfiI CCNNNNNNNGG 2 cut(s) 87, 1193
AflII CTTAAG 1 cut(s) 658
AgsI TTSAA 9 cut(s) 68, 301, 582, 817, 854, 945, 1059, 1117, 1283
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 870
AloI GAACNNNNNNTCC 2 cut(s) 678, 710
AluBI AGCT 5 cut(s) 118, 259, 1024, 1055, 1214
AluI AGCT 5 cut(s) 118, 259, 1024, 1055, 1214
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 5 cut(s) 212, 435, 644, 972, 1094
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 6 cut(s) 39, 54, 77, 1108, 1220, 1348
ApaI GGGCCC 1 cut(s) 58
ApeKI GCWGC 4 cut(s) 352, 766, 1367, 1420
ApoI RAATTY 4 cut(s) 434, 602, 904, 1117
Asp700I GAANNNNTTC 2 cut(s) 1155, 1278
AspS9I GGNCC 6 cut(s) 54, 55, 78, 1220, 1334, 1349
AsuHPI GGTGA 1 cut(s) 913
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 1334
BaeGI GKGCMC 2 cut(s) 58, 1312
BalI TGGCCA 1 cut(s) 1110
BanII GRGCYC 2 cut(s) 58, 120
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 4 cut(s) 364, 778, 1354, 1407
BccI CCATC 5 cut(s) 66, 89, 245, 925, 1241
BceAI ACGGC 2 cut(s) 26, 95
BciT130I CCWGG 1 cut(s) 872
BclI TGATCA 1 cut(s) 1425
BfaI CTAG 4 cut(s) 260, 269, 669, 1362
BfmI CTRYAG 1 cut(s) 1368
BfrI CTTAAG 1 cut(s) 658
BisI GCNGC 4 cut(s) 353, 767, 1368, 1421
BlsI GCNGC 4 cut(s) 354, 768, 1369, 1422
BmcAI AGTACT 1 cut(s) 1412
Bme1390I CCNGG 1 cut(s) 872
Bme18I GGWCC 1 cut(s) 1334
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 6 cut(s) 54, 55, 78, 1220, 1334, 1349
BmiI GGNNCC 3 cut(s) 55, 56, 1336
BmrFI CCNGG 1 cut(s) 872
BmrI ACTGGG 1 cut(s) 1346
BmsI GCATC 1 cut(s) 1159
BmuI ACTGGG 1 cut(s) 1346
BpmI CTGGAG 2 cut(s) 893, 1236
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 42, 133, 222
BsaXI ACNNNNNCTCC 6 cut(s) 678, 708, 866, 896, 1209, 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 1193
Bse1I ACTGG 5 cut(s) 1011, 1111, 1264, 1305, 1352
Bse3DI GCAATG 2 cut(s) 829, 1085
BseBI CCWGG 1 cut(s) 872
BseDI CCNNGG 3 cut(s) 42, 133, 222
BseGI GGATG 1 cut(s) 1252
BseLI CCNNNNNNNGG 2 cut(s) 87, 1193
BseMI GCAATG 2 cut(s) 829, 1085
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 5 cut(s) 1011, 1111, 1264, 1305, 1352
BseSI GKGCMC 2 cut(s) 58, 1312
BseXI GCAGC 4 cut(s) 364, 778, 1354, 1407
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 6 cut(s) 41, 56, 79, 1110, 1222, 1350
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 87, 1193
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 6 cut(s) 41, 56, 79, 1110, 1222, 1350
BsoBI CYCGRG 1 cut(s) 161
Bsp120I GGGCCC 1 cut(s) 54
Bsp1286I GDGCHC 3 cut(s) 58, 120, 1312
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 3 cut(s) 127, 158, 1040
BspANI GGCC 6 cut(s) 41, 56, 79, 1110, 1222, 1350
BspCNI CTCAG 1 cut(s) 750
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 3 cut(s) 55, 56, 1336
BspMAI CTGCAG 1 cut(s) 1372
BspPI GGATC 5 cut(s) 212, 435, 644, 972, 1094
BspTI CTTAAG 1 cut(s) 658
BsrBI CCGCTC 2 cut(s) 129, 160
BsrDI GCAATG 2 cut(s) 829, 1085
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 5 cut(s) 1011, 1111, 1264, 1305, 1352
BssECI CCNNGG 3 cut(s) 42, 133, 222
BssMI GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 872
Bst4CI ACNGT 5 cut(s) 328, 556, 987, 1182, 1235
Bst6I CTCTTC 1 cut(s) 641
BstACI GRCGYC 1 cut(s) 108
BstAFI CTTAAG 1 cut(s) 658
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstAUI TGTACA 1 cut(s) 882
BstC8I GCNNGC 4 cut(s) 35, 39, 1131, 1381
BstDEI CTNAG 1 cut(s) 758
BstDSI CCRYGG 3 cut(s) 42, 133, 222
BstF5I GGATG 1 cut(s) 1252
BstKTI GATC 7 cut(s) 196, 220, 430, 652, 967, 1102, 1428
BstMBI GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
BstMWI GCNNNNNNNGC 3 cut(s) 135, 1228, 1376
BstNI CCWGG 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 870
BstSFI CTRYAG 1 cut(s) 1368
BstSLI GKGCMC 2 cut(s) 58, 1312
BstV1I GCAGC 4 cut(s) 364, 778, 1354, 1407
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuRI GGCC 6 cut(s) 41, 56, 79, 1110, 1222, 1350
BtgI CCRYGG 3 cut(s) 42, 133, 222
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 1252
BtsIMutI CAGTG 7 cut(s) 413, 552, 867, 1018, 1231, 1312, 1359
Cac8I GCNNGC 4 cut(s) 35, 39, 1131, 1381
CciI TCATGA 1 cut(s) 931
Cfr13I GGNCC 6 cut(s) 54, 55, 78, 1220, 1334, 1349
CseI GACGC 2 cut(s) 101, 116
Csp6I GTAC 2 cut(s) 883, 1411
CspCI CAANNNNNGTGG 2 cut(s) 1327, 1362
CviAII CATG 8 cut(s) 223, 368, 560, 833, 926, 932, 1033, 1079
CviQI GTAC 2 cut(s) 883, 1411
DdeI CTNAG 1 cut(s) 758
DpnI GATC 7 cut(s) 195, 219, 429, 651, 966, 1101, 1427
DpnII GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
DraI TTTAAA 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 2 cut(s) 58, 120
Eco47I GGWCC 1 cut(s) 1334
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 4 cut(s) 300, 437, 585, 732
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 3 cut(s) 54, 1220, 1334
EcoRI GAATTC 1 cut(s) 1117
EcoRII CCWGG 1 cut(s) 870
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 2 cut(s) 58, 120
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 8 cut(s) 226, 371, 563, 836, 929, 935, 1036, 1082
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 637, 669
FaqI GGGAC 1 cut(s) 104
FatI CATG 8 cut(s) 222, 367, 559, 832, 925, 931, 1032, 1078
FbaI TGATCA 1 cut(s) 1425
Fnu4HI GCNGC 4 cut(s) 353, 767, 1368, 1421
FokI GGATG 1 cut(s) 1259
FriOI GRGCYC 2 cut(s) 58, 120
Fsp4HI GCNGC 4 cut(s) 353, 767, 1368, 1421
FspBI CTAG 4 cut(s) 260, 269, 669, 1362
GluI GCNGC 4 cut(s) 353, 767, 1368, 1421
GsuI CTGGAG 2 cut(s) 893, 1236
HaeIII GGCC 6 cut(s) 41, 56, 79, 1110, 1222, 1350
HgaI GACGC 2 cut(s) 101, 116
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 8 cut(s) 226, 371, 563, 836, 929, 935, 1036, 1082
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HinfI GANTC 2 cut(s) 301, 1082
HphI GGTGA 1 cut(s) 913
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 2 cut(s) 19, 466
Hpy188III TCNNGA 7 cut(s) 197, 431, 669, 742, 932, 998, 1332
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 2 cut(s) 23, 110
HpyAV CCTTC 4 cut(s) 440, 1200, 1268, 1436
HpyCH4III ACNGT 5 cut(s) 328, 556, 987, 1182, 1235
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 6 cut(s) 352, 705, 766, 1090, 1256, 1370
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 1228, 1376
HpyF3I CTNAG 1 cut(s) 758
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 8 cut(s) 226, 371, 563, 836, 929, 935, 1036, 1082
Ksp22I TGATCA 1 cut(s) 1425
Kzo9I GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 4 cut(s) 364, 778, 1354, 1407
LweI GCATC 1 cut(s) 1159
MaeI CTAG 4 cut(s) 260, 269, 669, 1362
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 472, 1357
MalI GATC 7 cut(s) 195, 219, 429, 651, 966, 1101, 1427
MbiI CCGCTC 2 cut(s) 129, 160
MboI GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
MboII GAAGA 5 cut(s) 56, 253, 578, 628, 912
MfeI CAATTG 2 cut(s) 451, 617
MhlI GDGCHC 3 cut(s) 58, 120, 1312
MlsI TGGCCA 1 cut(s) 1110
MluNI TGGCCA 1 cut(s) 1110
MmeI TCCRAC 2 cut(s) 42, 402
Mox20I TGGCCA 1 cut(s) 1110
MroXI GAANNNNTTC 2 cut(s) 1155, 1278
MscI TGGCCA 1 cut(s) 1110
MseI TTAA 8 cut(s) 123, 494, 501, 659, 731, 972, 1026, 1239
MslI CAYNNNNRTG 4 cut(s) 227, 372, 930, 1095
Msp20I TGGCCA 1 cut(s) 1110
MspA1I CMGCKG 1 cut(s) 1214
MspCI CTTAAG 1 cut(s) 658
MspR9I CCNGG 1 cut(s) 872
MunI CAATTG 2 cut(s) 451, 617
MvaI CCWGG 1 cut(s) 872
MwoI GCNNNNNNNGC 3 cut(s) 135, 1228, 1376
NcoI CCATGG 1 cut(s) 222
NdeII GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
NlaIII CATG 8 cut(s) 226, 371, 563, 836, 929, 935, 1036, 1082
NlaIV GGNNCC 3 cut(s) 55, 56, 1336
NmuCI GTSAC 1 cut(s) 472
PaeR7I CTCGAG 1 cut(s) 161
PagI TCATGA 1 cut(s) 931
PdmI GAANNNNTTC 2 cut(s) 1155, 1278
PfeI GAWTC 2 cut(s) 301, 1082
PkrI GCNGC 4 cut(s) 354, 768, 1369, 1422
PpuMI RGGWCCY 1 cut(s) 1334
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 1334
Psp6I CCWGG 1 cut(s) 870
PspGI CCWGG 1 cut(s) 870
PspN4I GGNNCC 3 cut(s) 55, 56, 1336
PspOMI GGGCCC 1 cut(s) 54
PspPI GGNCC 6 cut(s) 54, 55, 78, 1220, 1334, 1349
PspPPI RGGWCCY 1 cut(s) 1334
PspXI VCTCGAGB 1 cut(s) 161
PstI CTGCAG 1 cut(s) 1372
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 2 cut(s) 884, 1412
RsaNI GTAC 2 cut(s) 883, 1411
RseI CAYNNNNRTG 4 cut(s) 227, 372, 930, 1095
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 8 cut(s) 123, 494, 501, 659, 731, 972, 1026, 1239
SatI GCNGC 4 cut(s) 353, 767, 1368, 1421
Sau3AI GATC 7 cut(s) 193, 217, 427, 649, 964, 1099, 1425
Sau96I GGNCC 6 cut(s) 54, 55, 78, 1220, 1334, 1349
ScaI AGTACT 1 cut(s) 1412
ScrFI CCNGG 1 cut(s) 872
SduI GDGCHC 3 cut(s) 58, 120, 1312
SfaNI GCATC 1 cut(s) 1159
SfcI CTRYAG 1 cut(s) 1368
Sfr274I CTCGAG 1 cut(s) 161
SinI GGWCC 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 161
SmiI ATTTAAAT 1 cut(s) 732
SmiMI CAYNNNNRTG 4 cut(s) 227, 372, 930, 1095
SmlI CTYRAG 2 cut(s) 161, 658
SmoI CTYRAG 2 cut(s) 161, 658
SsiI CCGC 3 cut(s) 127, 158, 1040
SspMI CTAG 4 cut(s) 260, 269, 669, 1362
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 870
StyI CCWWGG 1 cut(s) 222
SwaI ATTTAAAT 1 cut(s) 732
TaaI ACNGT 5 cut(s) 328, 556, 987, 1182, 1235
TaiI ACGT 1 cut(s) 24
TaqI TCGA 5 cut(s) 162, 381, 487, 999, 1272
TatI WGTACW 2 cut(s) 882, 1410
TfiI GAWTC 2 cut(s) 301, 1082
Tru1I TTAA 8 cut(s) 123, 494, 501, 659, 731, 972, 1026, 1239
Tru9I TTAA 8 cut(s) 123, 494, 501, 659, 731, 972, 1026, 1239
TscAI CASTG 7 cut(s) 420, 559, 867, 1018, 1238, 1312, 1359
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 4 cut(s) 352, 766, 1367, 1420
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 4 cut(s) 321, 948, 1021, 1074
TspGWI ACGGA 2 cut(s) 1092, 1154
TspRI CASTG 7 cut(s) 420, 559, 867, 1018, 1238, 1312, 1359
Vha464I CTTAAG 1 cut(s) 658
VpaK11BI GGWCC 1 cut(s) 1334
XapI RAATTY 4 cut(s) 434, 602, 904, 1117
XbaI TCTAGA 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XhoI CTCGAG 1 cut(s) 161
XmnI GAANNNNTTC 2 cut(s) 1155, 1278
XspI CTAG 4 cut(s) 260, 269, 669, 1362
ZrmI AGTACT 1 cut(s) 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.