MD13G1036100.v1.1

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001947
Physical Location & Seq
Forward (+)
2516440 .. 2523296
6857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1036100.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGAATCAAAACCTCTACCTCTTCATCCTGATCTTCCTTCTCTCAGAAACCCCAGCATTTGCAGTGGACCTTTACTACCAAAACTGCAGCGTCCCCACAACCTGTGGCCGCCTAAACATCAGCTATCCGTTCTACATCCCAGGCAGGCAACAAGACTTCTGCGGCTACCCTGGTTTCCAGCTCTCCTGCCACGGCGGCGCAGACGACGACAAAGAAGAAGCCTACCCACTTCTCCAGTTGTCTGGAGACGATTACATAATTCACAACATCAGTTACGAAAGACAGACTCTTGTCGTCTCAAATGTTCTACTTTCGCACTACTTGGACAACTCTTCCTGCACTAATCTCTCACTGATCCATAATTTAACGCTCCCTAATGAACAGTTTGAGCTGGCTCCAAACCAAGATCAGTTCTTTCTGCTCCACAACTGCAGCCCTTCGTTTGTCGATTCTTTTCCAACGTACAAGATTGGTTGTAGTAACACTTCGGTTCTTGCTCTGCCTCGGCAGATATATCCGAAATTTGAAGGTCTTGGGGAGGTTGAAAAGTGTGGATCGAGTGAGGTGCCGGTGGCGCAAGGTGGAGGGTATGGGAATGACGAGGCGGCGGGAATGAAGGAAGTGTTGGGAAGAGGGTTTGAGATGAAATGGCTGGCTGGTGACTGCAGCGAATGCCAGAGGAGTGGAGGGCTATGTGGGTTTAACTACACCACCCACCTTTTTAGGTGCCTCTGCCCCAACAGGTCCTATTCTGTGTTCTGTAGGGGCGACGAAGGAGATAAGCAGTTGAACCTGAAGCTAGGACTAGGAATTGGCCTTGGATTTCCAGCACTTCTTGCTATATGCGTGTGTCTTCTGTTGCTTTACAAGAAAAAACGCTCTTCACTCTTCCTCTCAAGGAACATTTCTTCTCAGCCATACACAAATTCCGACATAGAAGGGGGCGGTGCCTACTGTGGTATCCCGGTCTTCAGCTACAGTGAACTCGATGAGGCAACGAACCACTTTGATAGCGAAAGCGAACTTGGAGATGGAGGTTTCGGAACTGTCTACTATGGAAAACTCAAAGATGGTCGGGAAGTTGCAGTCAAGCGCTTATATGAGCACAATTACAAACGCGTTGAACAGTTCATAAATGAAATTGAAATCCTCACCCGTCTGCGCCACAGAAACTTAGTCTCCCTTTATGGCTGCACCTCACGCCGCAGCCGTGAACTCCTCCTTGTTTACGAATACATTTCCAATGGAACCGTTGCTGATCATCTCCACGGAGACAGAGCAGATGGCTCACTCACTTGGCCTATACGTATGAGCATCGCCATTGAAACCGCCGACGCATTGTCCTACCTCCATGCTTCTGAAATTGTACATCGCGATGTGAAGACTAACAACATTCTGCTCGATGACAATTTCTGCGTTAAAGTTGCAGATTTTGGCCTCTCCCGGCTCTTCCCCATTGATGTCACGCATGTCTCAACCGCTCCACAAGGGACACCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

501

Amino Acids

55.75

Weight (kDa)

5.3

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 100 6.6e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 180 - 247 2.2e-13 Wall-associated receptor kinase C-terminal
Pkinase PF00069 337 - 489 7.8e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 339 - 484 2e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 565, 726, 947
AccBSI CCGCTC 1 cut(s) 1481
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 2 cut(s) 1119, 1374
AciI CCGC 9 cut(s) 109, 162, 195, 605, 608, 945, 1204, 1329, 1479
AclWI GGATC 2 cut(s) 349, 562
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 2 cut(s) 521, 925
AcuI CTGAAG 2 cut(s) 815, 955
AfaI GTAC 2 cut(s) 464, 1368
AfeI AGCGCT 1 cut(s) 1094
AflIII ACRYGT 1 cut(s) 1117
AgsI TTSAA 6 cut(s) 527, 545, 790, 1124, 1145, 1325
AhdI GACNNNNNGTC 1 cut(s) 1339
AjnI CCWGG 2 cut(s) 139, 169
AjuI GAANNNNNNNTTGG 4 cut(s) 608, 640, 1008, 1040
AleI CACNNNNGTG 1 cut(s) 1498
AluBI AGCT 5 cut(s) 123, 181, 391, 799, 975
AluI AGCT 5 cut(s) 123, 181, 391, 799, 975
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 5 cut(s) 240, 301, 1183, 1266, 1477
AlwI GGATC 2 cut(s) 349, 562
Aor51HI AGCGCT 1 cut(s) 1094
AoxI GGCC 4 cut(s) 106, 814, 1298, 1435
ApeKI GCWGC 5 cut(s) 87, 432, 666, 1191, 1206
ApoI RAATTY 2 cut(s) 521, 925
AspLEI GCGC 4 cut(s) 200, 577, 1095, 1164
AspS9I GGNCC 2 cut(s) 67, 744
AsuC2I CCSGG 2 cut(s) 965, 1444
AsuHPI GGTGA 2 cut(s) 671, 1144
AvaII GGWCC 2 cut(s) 67, 744
BaeI ACNNNNGTAYC 2 cut(s) 943, 976
BanI GGYRCC 3 cut(s) 565, 726, 947
BarI GAAGNNNNNNTAC 2 cut(s) 469, 501
BbsI GAAGAC 3 cut(s) 845, 961, 1388
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 5 cut(s) 99, 444, 678, 1178, 1218
BccI CCATC 3 cut(s) 1025, 1064, 1277
BceAI ACGGC 2 cut(s) 208, 1194
BcgI CGANNNNNNTGC 2 cut(s) 547, 581
BciT130I CCWGG 2 cut(s) 141, 171
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 1258
BcnI CCSGG 2 cut(s) 965, 1444
BcoDI GTCTC 5 cut(s) 240, 301, 1183, 1266, 1477
BfaI CTAG 2 cut(s) 800, 806
BfmI CTRYAG 5 cut(s) 85, 430, 664, 760, 976
BfoI RGCGCY 1 cut(s) 1096
BfuI GTATCC 1 cut(s) 971
BglI GCCNNNNNGGC 1 cut(s) 195
Bme1390I CCNGG 4 cut(s) 141, 171, 965, 1444
Bme18I GGWCC 2 cut(s) 67, 744
BmeRI GACNNNNNGTC 1 cut(s) 1339
BmgT120I GGNCC 2 cut(s) 67, 744
BmiI GGNNCC 5 cut(s) 396, 567, 728, 949, 1249
BmrFI CCNGG 4 cut(s) 141, 171, 965, 1444
BmsI GCATC 1 cut(s) 1323
BoxI GACNNNNGTC 1 cut(s) 290
BpiI GAAGAC 3 cut(s) 845, 961, 1388
BpmI CTGGAG 2 cut(s) 218, 264
BpuEI CTTGAG 1 cut(s) 880
BpuMI CCSGG 2 cut(s) 965, 1444
BsaAI YACGTR 1 cut(s) 1307
BsaJI CCNNGG 6 cut(s) 139, 169, 190, 503, 817, 1267
BsaWI WCCGGW 1 cut(s) 1495
BsaXI ACNNNNNCTCC 2 cut(s) 1163, 1193
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 1 cut(s) 235
BseBI CCWGG 2 cut(s) 141, 171
BseDI CCNNGG 6 cut(s) 139, 169, 190, 503, 817, 1267
BseGI GGATG 2 cut(s) 24, 135
BseMII CTCAG 2 cut(s) 57, 926
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 2 cut(s) 694, 1208
BseXI GCAGC 5 cut(s) 99, 444, 678, 1178, 1218
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 322, 1177
Bsh1236I CGCG 2 cut(s) 1119, 1374
BshFI GGCC 4 cut(s) 108, 816, 1300, 1437
BshNI GGYRCC 3 cut(s) 565, 726, 947
BsiHKAI GWGCWC 1 cut(s) 1107
BsiSI CCGG 4 cut(s) 569, 965, 1444, 1496
BslFI GGGAC 1 cut(s) 77
BsmAI GTCTC 5 cut(s) 240, 301, 1183, 1266, 1477
BsmBI CGTCTC 2 cut(s) 240, 301
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 4 cut(s) 108, 816, 1300, 1437
Bsp1286I GDGCHC 1 cut(s) 1107
Bsp1407I TGTACA 1 cut(s) 1366
Bsp143I GATC 5 cut(s) 30, 354, 406, 554, 1258
Bsp68I TCGCGA 1 cut(s) 1374
BspACI CCGC 9 cut(s) 109, 162, 195, 605, 608, 945, 1204, 1329, 1479
BspANI GGCC 4 cut(s) 108, 816, 1300, 1437
BspCNI CTCAG 2 cut(s) 56, 925
BspFNI CGCG 2 cut(s) 1119, 1374
BspLI GGNNCC 5 cut(s) 396, 567, 728, 949, 1249
BspMAI CTGCAG 3 cut(s) 89, 434, 668
BspPI GGATC 2 cut(s) 349, 562
BspQI GCTCTTC 2 cut(s) 886, 1454
BspT107I GGYRCC 3 cut(s) 565, 726, 947
BsrBI CCGCTC 1 cut(s) 1481
BsrFI RCCGGY 1 cut(s) 568
BsrGI TGTACA 1 cut(s) 1366
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 6 cut(s) 139, 169, 190, 503, 817, 1267
BssMI GATC 5 cut(s) 30, 354, 406, 554, 1258
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 2 cut(s) 141, 171
Bst4CI ACNGT 6 cut(s) 384, 956, 980, 1048, 1128, 1252
Bst6I CTCTTC 6 cut(s) 26, 337, 625, 886, 893, 1454
BstAPI GCANNNNNTGC 2 cut(s) 672, 836
BstAUI TGTACA 1 cut(s) 1366
BstBAI YACGTR 1 cut(s) 1307
BstC8I GCNNGC 3 cut(s) 146, 393, 654
BstDEI CTNAG 3 cut(s) 43, 912, 1174
BstDSI CCRYGG 2 cut(s) 190, 1267
BstF5I GGATG 2 cut(s) 24, 135
BstFNI CGCG 2 cut(s) 1119, 1374
BstH2I RGCGCY 1 cut(s) 1096
BstHHI GCGC 4 cut(s) 200, 577, 1095, 1164
BstKTI GATC 5 cut(s) 33, 357, 409, 557, 1261
BstMAI GTCTC 5 cut(s) 240, 301, 1183, 1266, 1477
BstMBI GATC 5 cut(s) 30, 354, 406, 554, 1258
BstMWI GCNNNNNNNGC 5 cut(s) 195, 574, 672, 836, 1200
BstNI CCWGG 2 cut(s) 141, 171
BstNSI RCATGY 1 cut(s) 1472
BstPAI GACNNNNGTC 1 cut(s) 290
BstSCI CCNGG 4 cut(s) 139, 169, 963, 1442
BstSFI CTRYAG 5 cut(s) 85, 430, 664, 760, 976
BstSNI TACGTA 1 cut(s) 1307
BstUI CGCG 2 cut(s) 1119, 1374
BstV1I GCAGC 5 cut(s) 99, 444, 678, 1178, 1218
BstV2I GAAGAC 3 cut(s) 845, 961, 1388
BstXI CCANNNNNNTGG 2 cut(s) 242, 683
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 4 cut(s) 108, 816, 1300, 1437
BtgI CCRYGG 2 cut(s) 190, 1267
BtgZI GCGATG 3 cut(s) 1300, 1355, 1389
BtsCI GGATG 2 cut(s) 24, 135
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 350, 985
BtuMI TCGCGA 1 cut(s) 1374
Cac8I GCNNGC 3 cut(s) 146, 393, 654
CfoI GCGC 4 cut(s) 200, 577, 1095, 1164
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 2 cut(s) 67, 744
CseI GACGC 2 cut(s) 79, 1343
Csp6I GTAC 2 cut(s) 463, 1367
CviAII CATG 2 cut(s) 1352, 1469
CviQI GTAC 2 cut(s) 463, 1367
DdeI CTNAG 3 cut(s) 43, 912, 1174
DpnI GATC 5 cut(s) 32, 356, 408, 556, 1260
DpnII GATC 5 cut(s) 30, 354, 406, 554, 1258
DriI GACNNNNNGTC 1 cut(s) 1339
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 6 cut(s) 26, 337, 625, 886, 893, 1454
Eam1105I GACNNNNNGTC 1 cut(s) 1339
EarI CTCTTC 6 cut(s) 26, 337, 625, 886, 893, 1454
Eco105I TACGTA 1 cut(s) 1307
Eco130I CCWWGG 1 cut(s) 817
Eco47I GGWCC 2 cut(s) 67, 744
Eco47III AGCGCT 1 cut(s) 1094
Eco57I CTGAAG 2 cut(s) 815, 955
EcoO109I RGGNCCY 1 cut(s) 744
EcoRII CCWGG 2 cut(s) 139, 169
EcoT14I CCWWGG 1 cut(s) 817
ErhI CCWWGG 1 cut(s) 817
Esp3I CGTCTC 2 cut(s) 240, 301
FaeI CATG 2 cut(s) 1355, 1472
FaqI GGGAC 1 cut(s) 77
FatI CATG 2 cut(s) 1351, 1468
FauI CCCGC 1 cut(s) 601
FbaI TGATCA 1 cut(s) 1258
FblI GTMKAC 1 cut(s) 1050
FokI GGATG 2 cut(s) 11, 122
FspBI CTAG 2 cut(s) 800, 806
GlaI GCGC 4 cut(s) 199, 576, 1094, 1163
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 2 cut(s) 218, 264
HaeII RGCGCY 1 cut(s) 1096
HaeIII GGCC 4 cut(s) 108, 816, 1300, 1437
HapII CCGG 4 cut(s) 569, 965, 1444, 1496
HgaI GACGC 2 cut(s) 79, 1343
HhaI GCGC 4 cut(s) 200, 577, 1095, 1164
Hin1II CATG 2 cut(s) 1355, 1472
Hin6I GCGC 4 cut(s) 198, 575, 1093, 1162
HinP1I GCGC 4 cut(s) 198, 575, 1093, 1162
HinfI GANTC 3 cut(s) 4, 286, 449
HpaII CCGG 4 cut(s) 569, 965, 1444, 1496
HphI GGTGA 2 cut(s) 671, 1144
Hpy166II GTNNAC 5 cut(s) 67, 983, 1051, 1214, 1228
Hpy188I TCNGA 5 cut(s) 46, 519, 931, 1043, 1360
Hpy188III TCNNGA 4 cut(s) 28, 243, 1076, 1373
Hpy8I GTNNAC 5 cut(s) 67, 983, 1051, 1214, 1228
Hpy99I CGWCG 3 cut(s) 209, 773, 1337
HpyAV CCTTC 6 cut(s) 47, 447, 521, 610, 767, 932
HpyCH4III ACNGT 6 cut(s) 384, 956, 980, 1048, 1128, 1252
HpyCH4IV ACGT 2 cut(s) 461, 1306
HpyCH4V TGCA 8 cut(s) 62, 87, 339, 432, 666, 1085, 1194, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 574, 672, 836, 1200
HpyF3I CTNAG 3 cut(s) 43, 912, 1174
HpySE526I ACGT 2 cut(s) 461, 1306
Hsp92II CATG 2 cut(s) 1355, 1472
HspAI GCGC 4 cut(s) 198, 575, 1093, 1162
Ksp22I TGATCA 1 cut(s) 1258
Kzo9I GATC 5 cut(s) 30, 354, 406, 554, 1258
LguI GCTCTTC 2 cut(s) 886, 1454
LmnI GCTCC 4 cut(s) 375, 400, 426, 1486
Lsp1109I GCAGC 5 cut(s) 99, 444, 678, 1178, 1218
LweI GCATC 1 cut(s) 1323
MaeI CTAG 2 cut(s) 800, 806
MaeII ACGT 2 cut(s) 461, 1306
MaeIII GTNAC 4 cut(s) 272, 479, 659, 1462
MalI GATC 5 cut(s) 32, 356, 408, 556, 1260
MbiI CCGCTC 1 cut(s) 1481
MboI GATC 5 cut(s) 30, 354, 406, 554, 1258
MhlI GDGCHC 1 cut(s) 1107
MluCI AATT 9 cut(s) 258, 361, 521, 810, 925, 1108, 1140, 1362, 1408
MluI ACGCGT 1 cut(s) 1117
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 2 cut(s) 482, 954
MseI TTAA 3 cut(s) 365, 702, 1419
MslI CAYNNNNRTG 2 cut(s) 1374, 1498
MspI CCGG 4 cut(s) 569, 965, 1444, 1496
MspR9I CCNGG 4 cut(s) 141, 171, 965, 1444
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 2 cut(s) 141, 171
MvnI CGCG 2 cut(s) 1119, 1374
MwoI GCNNNNNNNGC 5 cut(s) 195, 574, 672, 836, 1200
NciI CCSGG 2 cut(s) 965, 1444
NdeII GATC 5 cut(s) 30, 354, 406, 554, 1258
NlaIII CATG 2 cut(s) 1355, 1472
NlaIV GGNNCC 5 cut(s) 396, 567, 728, 949, 1249
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 659, 1462
NruI TCGCGA 1 cut(s) 1374
NspI RCATGY 1 cut(s) 1472
OliI CACNNNNGTG 1 cut(s) 1498
PciSI GCTCTTC 2 cut(s) 886, 1454
PcsI WCGNNNNNNNCGW 1 cut(s) 1207
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 2 cut(s) 4, 449
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 1307
PpuMI RGGWCCY 1 cut(s) 744
PshAI GACNNNNGTC 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 744
Psp6I CCWGG 2 cut(s) 139, 169
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 139, 169
PspN4I GGNNCC 5 cut(s) 396, 567, 728, 949, 1249
PspPI GGNCC 2 cut(s) 67, 744
PspPPI RGGWCCY 1 cut(s) 744
PstI CTGCAG 3 cut(s) 89, 434, 668
RruI TCGCGA 1 cut(s) 1374
RsaI GTAC 2 cut(s) 464, 1368
RsaNI GTAC 2 cut(s) 463, 1367
RseI CAYNNNNRTG 2 cut(s) 1374, 1498
SapI GCTCTTC 2 cut(s) 886, 1454
SaqAI TTAA 3 cut(s) 365, 702, 1419
Sau3AI GATC 5 cut(s) 30, 354, 406, 554, 1258
Sau96I GGNCC 2 cut(s) 67, 744
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 4 cut(s) 141, 171, 965, 1444
SduI GDGCHC 1 cut(s) 1107
SfaNI GCATC 1 cut(s) 1323
SfcI CTRYAG 5 cut(s) 85, 430, 664, 760, 976
SinI GGWCC 2 cut(s) 67, 744
SmiMI CAYNNNNRTG 2 cut(s) 1374, 1498
SmlI CTYRAG 1 cut(s) 895
SmoI CTYRAG 1 cut(s) 895
SnaBI TACGTA 1 cut(s) 1307
Sse9I AATT 9 cut(s) 258, 361, 521, 810, 925, 1108, 1140, 1362, 1408
SsiI CCGC 9 cut(s) 109, 162, 195, 605, 608, 945, 1204, 1329, 1479
SspMI CTAG 2 cut(s) 800, 806
StyD4I CCNGG 4 cut(s) 139, 169, 963, 1442
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 6 cut(s) 384, 956, 980, 1048, 1128, 1252
TaiI ACGT 2 cut(s) 464, 1309
TaqI TCGA 4 cut(s) 447, 557, 987, 1401
TasI AATT 9 cut(s) 258, 361, 521, 810, 925, 1108, 1140, 1362, 1408
TatI WGTACW 1 cut(s) 1366
TauI GCSGC 5 cut(s) 111, 165, 198, 608, 1206
TfiI GAWTC 2 cut(s) 4, 449
Tru1I TTAA 3 cut(s) 365, 702, 1419
Tru9I TTAA 3 cut(s) 365, 702, 1419
TscAI CASTG 3 cut(s) 69, 357, 985
TseFI GTSAC 2 cut(s) 659, 1462
TseI GCWGC 5 cut(s) 87, 432, 666, 1191, 1206
Tsp45I GTSAC 2 cut(s) 659, 1462
TspDTI ATGAA 7 cut(s) 13, 17, 393, 629, 659, 1120, 1152
TspGWI ACGGA 2 cut(s) 117, 1284
TspRI CASTG 3 cut(s) 69, 357, 985
VpaK11BI GGWCC 2 cut(s) 67, 744
XapI RAATTY 2 cut(s) 521, 925
XceI RCATGY 1 cut(s) 1472
XmiI GTMKAC 1 cut(s) 1050
XspI CTAG 2 cut(s) 800, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.