Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001947

Physical Location & Seq

Genomic Coordinates Forward (+)

55492561 .. 55495288

Gene Structure 2728 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G028860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGGCTACCGTGGCTTTGTTTGTTTTCTGTGTTTTCACTCTTCTCGTTCTTCACTCTGCTGAAGATAAGAGAGACGAAATTTGTCCAGCCCTCTTCTGTAATGACAGAGTAGGCAGCATCTACTTTCCCTTCAGAAGCACAACACAGAGTCCTGCATGCGGATTGTACACCGTTAATTGTTCTGACAGGCCTAATCCGAAGATCCAACTCAAAGATGGAGGATACTGGTTTCAACTGAAAGTCATGACTCGTACGCCTTCCATTTTTATCGATGACAGAGGGCTTCAAAAGCGCATAGAGCGCTTGAAAACAGATACATCTCATACATGTGATGATGATGTTTTCAATGATTTAAGTCTTCCAGGCCCGTCTCCCATCCTTAATATATCCACAAACAACCTTACCCTGTTCAGATGCAACAACAGCATGAACAAAACTTTCGAGCATCCTAACTATGCCTGCAGGAGTGTAAAGTATACATACTATACTGTATCAAATGATAGTTTAGCGTGCTCAGCCATTCAGATCCCCAAACATCCAGATTTTCGTTCTAATGTCTATAATCCTCATGCATTGACTGCTGAATTCTCCCTTCTAGTGCAATTTACCAAAGAATGTGATCACTGCTATACTAGCAATGGTAAATGCTTGGTTGACAACGGAGAATTCAAATGTGCCAAAGCAAAAAAAGAAGCTCCATTGGGATTGAAGCTAGGACTTGGTGTTGGACTTGGAGGTGCAGCATTCCTTGGAGTCCTTGCAGCATGCTTGTTTCTTTTCAGGCGTTACAAAAAAAAGCTCGCTTCTTCAAACGTATTCTCCCGGAACATTTCTTCTCAGCCCTACTCAAAATCAGACCTAGAAGGGGGCAATGCCTACTTTGGTGTTTCGGTCTTCACCTACGAGGAACTTGAAGAAGCCACTAATCATTTTGACAGTGAAAAAGAGCTTGGAGATGGAGGTTTTGGAACTGTCTACTATGGCAAACTCAAAGATGGCCGGGAAGTTGCAGTCAAGCGTTTGTACGAGCACAATTACAAACGTGTGGAACAGTTTATGAATGAAATTGAAATTCTCACCCGTCTGCGCCACAAAAACTTAGTCTCCCTCTATGGTTGCACTTCACGCCGCAGCCGTGAACTCATCCTTGTCTATGAATACATTCCCAATGGCACTGTTGCTGATCATCTCCACGGCGACCTAGCAGAACGTGGTTTACTCACGTGGCCTATTCGTATGAGCATTGCCATAGAAACTGCCAATGCATTGTCTTACCTCCATGTTACTGATATTGTGCATCGTGATGTGAAGACTACCAACATTCTTCTTGATAACAACTTCTGTGTTAAAGTTGCAGATTTTGGGCTCTCAAGGCTTTTTCCCCTTGATGTCACTCATGTCTCTACTGCTCCACAAGGGACCCCTGGTTATGTTGATCCAGAGTATCACCAGTGTTACCAGCTAACTAGTAAGAGTGATGTTTATAGTTTTGGGGTTGTCCTCATTGAGCTGATATCATCTCTGCCAGCAGTTGATATAACTAGGCATAGGCACGAGATCAATTTGTCTAACATAGCTATAAACAAGATTCAAGGAGGTTTATACAGTGAGCTGGTAGATTCATGTCTTGGGTTTGAGTCAGATCATGAAGTTAAAAGGATGACAATTGCAGTGGCAGAGTTGGCTTTCCAATGTTTGCAGCAGGACAATGATGCAAGGCCTACCATGTCTGAGGTTTTGGAAACTTTGCAGATGATTGAAAGTGGAAAAGATGTTCCAGAGAATTTGAAGGAGGACTTTGGTGATGTTGAGATGAGAAAGAGTATACAGCCACCACCTTCACCAGAATGTGATGAGCTTCAGTTGTTGAAGAACATGAGGCCACCATTTTCACCGATTTCCGTGACCCAAAACTGGCCTAGCACTAGGTCTGATACACCTAATGCCAGTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

651

Amino Acids

73.12

Weight (kDa)

6.01

Isoelectric Point (pI)

47.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	312 - 581	2.7e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	314 - 583	1.2e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001947)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25390 AT1G25390
fragaria_vesca	FvH4_4g26830 FvH4_4g26840 FvH4_4g26841 FvH4_4g26841
malus_domestica	MD13G1036100.v1.1 MD16G1037800.v1.1 MD16G1038800.v1.1
prunus_persica	Prupe.1G315200_v2.0.a1 Prupe.1G315900_v2.0.a1 Prupe.1G315900_v2.0.a1 Prupe.1G315900_v2.0.a1
pyrus_communis	pycom13g03150
rosa_chinensis	RchiOBHm_Chr4g0434491 RchiOBHm_Chr4g0434531 RchiOBHm_Chr4g0434541 RchiOBHm_Chr4g0434651
rosa_laevigata	RLG00000006663 RLG00000006664 RLG00000006665
rosa_multiflora	Rmu_co8505535.1_g000001 Rmu_sc0005783.1_g000005 Rmu_sc0005783.1_g000006 Rmu_sc0005783.1_g000008 Rmu_sc0005899.1_g000015 Rmu_sc0008439.1_g000007
rosa_roxburghii	Rroxscaffold_5G00375850
rosa_rugosa	Rorug04G0277200 Rorug04G0277200 Rorug04G0277200
rosa_samantha	Rh4AG333000 Rh4AG333900 Rh4BG340700 Rh4BG340800 Rh4BG341100 Rh4BG341600 Rh4BG341900 Rh4BG342100 Rh4BG342300 Rh4DG336000
rosa_wichuraiana	Rw4G028860 Rw4G028870 Rw4G028880 Rw4G028890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	476, 975, 1825
AciI	CCGC	2 cut(s)	159, 1129
AclWI	GGATC	3 cut(s)	196, 520, 1430
AcoI	YGGCCR	1 cut(s)	997
AcsI	RAATTY	5 cut(s)	78, 584, 665, 1071, 1783
AcuI	CTGAAG	3 cut(s)	81, 115, 1844
AcvI	CACGTG	1 cut(s)	1224
AfaI	GTAC	3 cut(s)	167, 253, 1025
AfeI	AGCGCT	1 cut(s)	302
AfiI	CCNNNNNNNGG	3 cut(s)	158, 865, 1914
AflIII	ACRYGT	2 cut(s)	326, 1042
AhlI	ACTAGT	1 cut(s)	1466
AjnI	CCWGG	2 cut(s)	361, 1423
AjuI	GAANNNNNNNTTGG	2 cut(s)	933, 965
AloI	GAACNNNNNNTCC	2 cut(s)	1758, 1790
AluBI	AGCT	9 cut(s)	695, 712, 799, 949, 1462, 1510, 1577, 1612, 1858
AluI	AGCT	9 cut(s)	695, 712, 799, 949, 1462, 1510, 1577, 1612, 1858
Alw21I	GWGCWC	2 cut(s)	515, 1032
Alw26I	GTCTC	4 cut(s)	66, 375, 1108, 1405
AlwI	GGATC	3 cut(s)	196, 520, 1430
AlwNI	CAGNNNCTG	1 cut(s)	1953
Aor51HI	AGCGCT	1 cut(s)	302
AoxI	GGCC	7 cut(s)	188, 364, 997, 1226, 1718, 1880, 1916
ApeKI	GCWGC	5 cut(s)	114, 740, 761, 1131, 1699
ApoI	RAATTY	5 cut(s)	78, 584, 665, 1071, 1783
ArsI	GACNNNNNNTTYG	2 cut(s)	1086, 1118
Asp700I	GAANNNNTTC	1 cut(s)	1161
AspLEI	GCGC	3 cut(s)	294, 303, 1089
AspS9I	GGNCC	2 cut(s)	365, 1419
AsuC2I	CCSGG	2 cut(s)	823, 1001
AsuHPI	GGTGA	6 cut(s)	889, 1069, 1439, 1814, 1833, 1884
AvaII	GGWCC	1 cut(s)	1419
BanII	GRGCYC	1 cut(s)	1368
BauI	CACGAG	1 cut(s)	1553
BbrPI	CACGTG	1 cut(s)	1224
BbsI	GAAGAC	3 cut(s)	350, 886, 1316
Bbv12I	GWGCWC	2 cut(s)	515, 1032
BbvI	GCAGC	5 cut(s)	126, 752, 773, 1143, 1711
BccI	CCATC	4 cut(s)	209, 383, 950, 989
BceAI	ACGGC	2 cut(s)	1119, 1210
BciT130I	CCWGG	2 cut(s)	363, 1425
BciVI	GTATCC	1 cut(s)	215
BclI	TGATCA	2 cut(s)	619, 1183
BcnI	CCSGG	2 cut(s)	823, 1001
BcoDI	GTCTC	4 cut(s)	66, 375, 1108, 1405
BcuI	ACTAGT	1 cut(s)	1466
BfaI	CTAG	9 cut(s)	596, 633, 713, 860, 1202, 1467, 1542, 1920, 1926
BfmI	CTRYAG	1 cut(s)	460
BfoI	RGCGCY	1 cut(s)	304
BfuI	GTATCC	1 cut(s)	215
BisI	GCNGC	6 cut(s)	115, 741, 762, 1129, 1132, 1700
BlpI	GCTNAGC	1 cut(s)	514
BlsI	GCNGC	6 cut(s)	116, 742, 763, 1130, 1133, 1701
Bme1390I	CCNGG	4 cut(s)	363, 823, 1001, 1425
Bme18I	GGWCC	1 cut(s)	1419
BmgT120I	GGNCC	2 cut(s)	365, 1419
BmiI	GGNNCC	2 cut(s)	1420, 1421
BmrFI	CCNGG	4 cut(s)	363, 823, 1001, 1425
BmsI	GCATC	5 cut(s)	126, 404, 454, 1306, 1702
BpiI	GAAGAC	3 cut(s)	350, 886, 1316
Bpu1102I	GCTNAGC	1 cut(s)	514
BpuEI	CTTGAG	1 cut(s)	1354
BpuMI	CCSGG	2 cut(s)	823, 1001
Bsa29I	ATCGAT	1 cut(s)	270
BsaAI	YACGTR	1 cut(s)	1224
BsaJI	CCNNGG	4 cut(s)	9, 748, 1192, 1423
BsaXI	ACNNNNNCTCC	4 cut(s)	803, 833, 1088, 1118
Bsc4I	CCNNNNNNNGG	3 cut(s)	158, 865, 1914
Bse1I	ACTGG	4 cut(s)	230, 1450, 1919, 1947
Bse3DI	GCAATG	3 cut(s)	643, 877, 1242
BseBI	CCWGG	2 cut(s)	363, 1425
BseCI	ATCGAT	1 cut(s)	270
BseDI	CCNNGG	4 cut(s)	9, 748, 1192, 1423
BseGI	GGATG	5 cut(s)	375, 445, 535, 1143, 1665
BseLI	CCNNNNNNNGG	3 cut(s)	158, 865, 1914
BseMI	GCAATG	3 cut(s)	643, 877, 1242
BseMII	CTCAG	3 cut(s)	528, 851, 1722
BseNI	ACTGG	4 cut(s)	230, 1450, 1919, 1947
BseXI	GCAGC	5 cut(s)	126, 752, 773, 1143, 1711
BsgI	GTGCAG	1 cut(s)	759
BshFI	GGCC	7 cut(s)	190, 366, 999, 1228, 1720, 1882, 1918
BshVI	ATCGAT	1 cut(s)	270
BsiHKAI	GWGCWC	2 cut(s)	515, 1032
BsiSI	CCGG	2 cut(s)	823, 1000
BsiWI	CGTACG	1 cut(s)	251
BslFI	GGGAC	1 cut(s)	1432
BslI	CCNNNNNNNGG	3 cut(s)	158, 865, 1914
BsmAI	GTCTC	4 cut(s)	66, 375, 1108, 1405
BsmBI	CGTCTC	2 cut(s)	66, 375
BsmFI	GGGAC	1 cut(s)	1432
BsmI	GAATGC	1 cut(s)	743
BsnI	GGCC	7 cut(s)	190, 366, 999, 1228, 1720, 1882, 1918
Bsp1286I	GDGCHC	3 cut(s)	515, 1032, 1368
Bsp1407I	TGTACA	1 cut(s)	165
Bsp143I	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
Bsp1720I	GCTNAGC	1 cut(s)	514
BspACI	CCGC	2 cut(s)	159, 1129
BspANI	GGCC	7 cut(s)	190, 366, 999, 1228, 1720, 1882, 1918
BspCNI	CTCAG	3 cut(s)	527, 850, 1723
BspDI	ATCGAT	1 cut(s)	270
BspHI	TCATGA	2 cut(s)	243, 1645
BspLI	GGNNCC	2 cut(s)	1420, 1421
BspMAI	CTGCAG	1 cut(s)	464
BspPI	GGATC	3 cut(s)	196, 520, 1430
BsrDI	GCAATG	3 cut(s)	643, 877, 1242
BsrGI	TGTACA	1 cut(s)	165
BsrI	ACTGG	4 cut(s)	230, 1450, 1919, 1947
BssECI	CCNNGG	4 cut(s)	9, 748, 1192, 1423
BssMI	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
BssNAI	GTATAC	2 cut(s)	477, 1826
BssSI	CACGAG	1 cut(s)	1553
BssT1I	CCWWGG	1 cut(s)	748
Bst1107I	GTATAC	2 cut(s)	477, 1826
Bst2BI	CACGAG	1 cut(s)	1553
Bst2UI	CCWGG	2 cut(s)	363, 1425
Bst4CI	ACNGT	8 cut(s)	10, 172, 490, 938, 973, 1053, 1177, 1607
Bst6I	CTCTTC	2 cut(s)	45, 98
BstAPI	GCANNNNNTGC	1 cut(s)	578
BstAUI	TGTACA	1 cut(s)	165
BstBAI	YACGTR	1 cut(s)	1224
BstC8I	GCNNGC	6 cut(s)	157, 460, 511, 766, 801, 1527
BstDEI	CTNAG	4 cut(s)	514, 837, 1099, 1731
BstDSI	CCRYGG	2 cut(s)	9, 1192
BstF5I	GGATG	5 cut(s)	375, 445, 535, 1143, 1665
BstH2I	RGCGCY	1 cut(s)	304
BstHHI	GCGC	3 cut(s)	294, 303, 1089
BstKTI	GATC	7 cut(s)	204, 528, 622, 1186, 1438, 1560, 1645
BstMAI	GTCTC	4 cut(s)	66, 375, 1108, 1405
BstMBI	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
BstNI	CCWGG	2 cut(s)	363, 1425
BstNSI	RCATGY	3 cut(s)	159, 330, 768
BstSCI	CCNGG	4 cut(s)	361, 821, 999, 1423
BstSFI	CTRYAG	1 cut(s)	460
BstV1I	GCAGC	5 cut(s)	126, 752, 773, 1143, 1711
BstV2I	GAAGAC	3 cut(s)	350, 886, 1316
BstX2I	RGATCY	2 cut(s)	201, 525
BstYI	RGATCY	2 cut(s)	201, 525
BstZ17I	GTATAC	2 cut(s)	477, 1826
Bsu15I	ATCGAT	1 cut(s)	270
BsuI	GTATCC	1 cut(s)	215
BsuRI	GGCC	7 cut(s)	190, 366, 999, 1228, 1720, 1882, 1918
BsuTUI	ATCGAT	1 cut(s)	270
BtgI	CCRYGG	2 cut(s)	9, 1192
BtsCI	GGATG	5 cut(s)	375, 445, 535, 1143, 1665
BtsI	GCAGTG	2 cut(s)	622, 1677
BtsIMutI	CAGTG	6 cut(s)	622, 943, 1173, 1457, 1612, 1677
Cac8I	GCNNGC	6 cut(s)	157, 460, 511, 766, 801, 1527
CaiI	CAGNNNCTG	1 cut(s)	1953
CciI	TCATGA	2 cut(s)	243, 1645
CfoI	GCGC	3 cut(s)	294, 303, 1089
Cfr13I	GGNCC	2 cut(s)	365, 1419
ClaI	ATCGAT	1 cut(s)	270
Csp6I	GTAC	3 cut(s)	166, 252, 1024
CspCI	CAANNNNNGTGG	2 cut(s)	1653, 1688
CviQI	GTAC	3 cut(s)	166, 252, 1024
DdeI	CTNAG	4 cut(s)	514, 837, 1099, 1731
DpnI	GATC	7 cut(s)	203, 527, 621, 1185, 1437, 1559, 1644
DpnII	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
EaeI	YGGCCR	1 cut(s)	997
Eam1104I	CTCTTC	2 cut(s)	45, 98
EarI	CTCTTC	2 cut(s)	45, 98
Eco130I	CCWWGG	1 cut(s)	748
Eco147I	AGGCCT	2 cut(s)	190, 1720
Eco24I	GRGCYC	1 cut(s)	1368
Eco32I	GATATC	1 cut(s)	1515
Eco47I	GGWCC	1 cut(s)	1419
Eco47III	AGCGCT	1 cut(s)	302
Eco57I	CTGAAG	3 cut(s)	81, 115, 1844
Eco72I	CACGTG	1 cut(s)	1224
EcoO109I	RGGNCCY	1 cut(s)	1419
EcoRI	GAATTC	2 cut(s)	584, 665
EcoRII	CCWGG	2 cut(s)	361, 1423
EcoRV	GATATC	1 cut(s)	1515
EcoT14I	CCWWGG	1 cut(s)	748
EcoT22I	ATGCAT	2 cut(s)	574, 1267
EcoT38I	GRGCYC	1 cut(s)	1368
ErhI	CCWWGG	1 cut(s)	748
Esp3I	CGTCTC	2 cut(s)	66, 375
FalI	AAGNNNNNCTT	2 cut(s)	1781, 1813
FaqI	GGGAC	1 cut(s)	1432
FbaI	TGATCA	2 cut(s)	619, 1183
FblI	GTMKAC	3 cut(s)	476, 975, 1825
Fnu4HI	GCNGC	6 cut(s)	115, 741, 762, 1129, 1132, 1700
FokI	GGATG	5 cut(s)	362, 432, 522, 1130, 1672
FriOI	GRGCYC	1 cut(s)	1368
Fsp4HI	GCNGC	6 cut(s)	115, 741, 762, 1129, 1132, 1700
FspBI	CTAG	9 cut(s)	596, 633, 713, 860, 1202, 1467, 1542, 1920, 1926
GlaI	GCGC	3 cut(s)	293, 302, 1088
GluI	GCNGC	6 cut(s)	115, 741, 762, 1129, 1132, 1700
HaeII	RGCGCY	1 cut(s)	304
HaeIII	GGCC	7 cut(s)	190, 366, 999, 1228, 1720, 1882, 1918
HapII	CCGG	2 cut(s)	823, 1000
HhaI	GCGC	3 cut(s)	294, 303, 1089
Hin6I	GCGC	3 cut(s)	292, 301, 1087
HinP1I	GCGC	3 cut(s)	292, 301, 1087
HincII	GTYRAC	1 cut(s)	655
HindII	GTYRAC	1 cut(s)	655
HinfI	GANTC	6 cut(s)	148, 247, 753, 1588, 1619, 1637
HpaII	CCGG	2 cut(s)	823, 1000
HphI	GGTGA	6 cut(s)	889, 1069, 1439, 1814, 1833, 1884
Hpy166II	GTNNAC	7 cut(s)	168, 477, 655, 976, 1139, 1217, 1826
Hpy188I	TCNGA	9 cut(s)	134, 184, 198, 413, 525, 856, 1642, 1732, 1933
Hpy188III	TCNNGA	7 cut(s)	244, 539, 1301, 1328, 1439, 1646, 1778
Hpy8I	GTNNAC	7 cut(s)	168, 477, 655, 976, 1139, 1217, 1826
HpyAV	CCTTC	6 cut(s)	139, 267, 602, 857, 1783, 1848
HpyCH4III	ACNGT	8 cut(s)	10, 172, 490, 938, 973, 1053, 1177, 1607
HpyCH4IV	ACGT	4 cut(s)	813, 1042, 1210, 1223
HpyF3I	CTNAG	4 cut(s)	514, 837, 1099, 1731
HpySE526I	ACGT	4 cut(s)	813, 1042, 1210, 1223
HspAI	GCGC	3 cut(s)	292, 301, 1087
KflI	GGGWCCC	1 cut(s)	1419
Ksp22I	TGATCA	2 cut(s)	619, 1183
Kzo9I	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
LmnI	GCTCC	2 cut(s)	700, 1414
Lsp1109I	GCAGC	5 cut(s)	126, 752, 773, 1143, 1711
LweI	GCATC	5 cut(s)	126, 404, 454, 1306, 1702
MaeI	CTAG	9 cut(s)	596, 633, 713, 860, 1202, 1467, 1542, 1920, 1926
MaeII	ACGT	4 cut(s)	813, 1042, 1210, 1223
MaeIII	GTNAC	5 cut(s)	785, 1282, 1390, 1454, 1903
MalI	GATC	7 cut(s)	203, 527, 621, 1185, 1437, 1559, 1644
MboI	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
MfeI	CAATTG	1 cut(s)	1665
MflI	RGATCY	2 cut(s)	201, 525
MhlI	GDGCHC	3 cut(s)	515, 1032, 1368
MlyI	GAGTC	4 cut(s)	157, 241, 762, 1646
MmeI	TCCRAC	2 cut(s)	229, 706
Mph1103I	ATGCAT	2 cut(s)	574, 1267
MroXI	GAANNNNTTC	1 cut(s)	1161
MseI	TTAA	5 cut(s)	174, 353, 381, 1347, 1653
MslI	CAYNNNNRTG	3 cut(s)	327, 1302, 1846
MspI	CCGG	2 cut(s)	823, 1000
MspR9I	CCNGG	4 cut(s)	363, 823, 1001, 1425
MunI	CAATTG	1 cut(s)	1665
Mva1269I	GAATGC	1 cut(s)	743
MvaI	CCWGG	2 cut(s)	363, 1425
NciI	CCSGG	2 cut(s)	823, 1001
NdeII	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
NlaIV	GGNNCC	2 cut(s)	1420, 1421
NmuCI	GTSAC	2 cut(s)	1390, 1903
NsiI	ATGCAT	2 cut(s)	574, 1267
NspI	RCATGY	3 cut(s)	159, 330, 768
PaeI	GCATGC	2 cut(s)	159, 768
PagI	TCATGA	2 cut(s)	243, 1645
PceI	AGGCCT	2 cut(s)	190, 1720
PciI	ACATGT	1 cut(s)	326
PcsI	WCGNNNNNNNCGW	1 cut(s)	1132
PctI	GAATGC	1 cut(s)	743
PdmI	GAANNNNTTC	1 cut(s)	1161
PfeI	GAWTC	2 cut(s)	1588, 1619
Pfl23II	CGTACG	1 cut(s)	251
PfoI	TCCNGGA	1 cut(s)	821
PkrI	GCNGC	6 cut(s)	116, 742, 763, 1130, 1133, 1701
PleI	GAGTC	4 cut(s)	156, 241, 761, 1645
PmaCI	CACGTG	1 cut(s)	1224
PmlI	CACGTG	1 cut(s)	1224
PpsI	GAGTC	4 cut(s)	156, 241, 761, 1645
Ppu21I	YACGTR	1 cut(s)	1224
PpuMI	RGGWCCY	1 cut(s)	1419
PscI	ACATGT	1 cut(s)	326
Psp5II	RGGWCCY	1 cut(s)	1419
Psp6I	CCWGG	2 cut(s)	361, 1423
PspCI	CACGTG	1 cut(s)	1224
PspGI	CCWGG	2 cut(s)	361, 1423
PspLI	CGTACG	1 cut(s)	251
PspN4I	GGNNCC	2 cut(s)	1420, 1421
PspPI	GGNCC	2 cut(s)	365, 1419
PspPPI	RGGWCCY	1 cut(s)	1419
PsrI	GAACNNNNNNTAC	2 cut(s)	1200, 1232
PstI	CTGCAG	1 cut(s)	464
PstNI	CAGNNNCTG	1 cut(s)	1953
PsuI	RGATCY	2 cut(s)	201, 525
RsaI	GTAC	3 cut(s)	167, 253, 1025
RsaNI	GTAC	3 cut(s)	166, 252, 1024
RseI	CAYNNNNRTG	3 cut(s)	327, 1302, 1846
SaqAI	TTAA	5 cut(s)	174, 353, 381, 1347, 1653
SatI	GCNGC	6 cut(s)	115, 741, 762, 1129, 1132, 1700
Sau3AI	GATC	7 cut(s)	201, 525, 619, 1183, 1435, 1557, 1642
Sau96I	GGNCC	2 cut(s)	365, 1419
SbfI	CCTGCAGG	1 cut(s)	464
SchI	GAGTC	4 cut(s)	157, 241, 762, 1646
ScrFI	CCNGG	4 cut(s)	363, 823, 1001, 1425
SdaI	CCTGCAGG	1 cut(s)	464
SduI	GDGCHC	3 cut(s)	515, 1032, 1368
SfaNI	GCATC	5 cut(s)	126, 404, 454, 1306, 1702
SfcI	CTRYAG	1 cut(s)	460
SinI	GGWCC	1 cut(s)	1419
SmiMI	CAYNNNNRTG	3 cut(s)	327, 1302, 1846
SmlI	CTYRAG	1 cut(s)	1369
SmoI	CTYRAG	1 cut(s)	1369
SpeI	ACTAGT	1 cut(s)	1466
SphI	GCATGC	2 cut(s)	159, 768
Sse8387I	CCTGCAGG	1 cut(s)	464
SseBI	AGGCCT	2 cut(s)	190, 1720
SsiI	CCGC	2 cut(s)	159, 1129
SspMI	CTAG	9 cut(s)	596, 633, 713, 860, 1202, 1467, 1542, 1920, 1926
StuI	AGGCCT	2 cut(s)	190, 1720
StyD4I	CCNGG	4 cut(s)	361, 821, 999, 1423
StyI	CCWWGG	1 cut(s)	748
TaaI	ACNGT	8 cut(s)	10, 172, 490, 938, 973, 1053, 1177, 1607
TaiI	ACGT	4 cut(s)	816, 1045, 1213, 1226
TaqI	TCGA	2 cut(s)	270, 441
TaqII	GACCGA	1 cut(s)	880
TatI	WGTACW	1 cut(s)	165
TauI	GCSGC	1 cut(s)	1131
TfiI	GAWTC	2 cut(s)	1588, 1619
Tru1I	TTAA	5 cut(s)	174, 353, 381, 1347, 1653
Tru9I	TTAA	5 cut(s)	174, 353, 381, 1347, 1653
TscAI	CASTG	6 cut(s)	629, 943, 1180, 1457, 1612, 1677
TseFI	GTSAC	2 cut(s)	1390, 1903
TseI	GCWGC	5 cut(s)	114, 740, 761, 1131, 1699
Tsp45I	GTSAC	2 cut(s)	1390, 1903
TspDTI	ATGAA	6 cut(s)	443, 1073, 1077, 1170, 1611, 1662
TspGWI	ACGGA	2 cut(s)	675, 1891
TspRI	CASTG	6 cut(s)	629, 943, 1180, 1457, 1612, 1677
VpaK11BI	GGWCC	1 cut(s)	1419
XapI	RAATTY	5 cut(s)	78, 584, 665, 1071, 1783
XceI	RCATGY	3 cut(s)	159, 330, 768
XcmI	CCANNNNNNNNNTGG	1 cut(s)	212
XmiI	GTMKAC	3 cut(s)	476, 975, 1825
XmnI	GAANNNNTTC	1 cut(s)	1161
XspI	CTAG	9 cut(s)	596, 633, 713, 860, 1202, 1467, 1542, 1920, 1926
Zsp2I	ATGCAT	2 cut(s)	574, 1267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.