MD16G1037800.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001947
Physical Location & Seq
Forward (+)
2709469 .. 2710215
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1037800.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGCTTCGGTGGCTTTGTTTATTCTCTCCGTTCTTGCTCACCTTGTGGTTCTGCACTCTGCTGAATATTCAGAAGCGGAACCCTCCAAACATTGTCCAGGCCACTTCTGCAGTGACATAGCAGGCAACATCAGCTTCCCCTTCAAAAGCCCGGGAGATCCTCCTGAATGCGGATTGTTCACAGTTGATTGTTCAGAACCCGATCGGCCAAAGATCCAATTGAAAGAGGGGGGATACTGGCATGAATTCCAAGGCTCTTTATTTTCACCTTCGATTTCTATCAATGACAGAGACCTTGCCGACCGCTTAGAAAAGGATCGCTGCGATGATGGAGTTTTCGACGACTTGAGTCTTCCCAGCGCTTCTCCAATCGTTGAAGTACCATTATTCACACACGATCTGACCCTTTTCAAATGCAACACCACCTTAGACAAAAATTCTTACCCACAACTTAATTATGGCTGCGGAAATGCTAACCATACTTATTATACCGCTTCATTTAGCAGTAGTTTAACTAACCCCCCGCCGCCAGGGTGTGCAACCATTCAGGTCCCCATAGTTCCGTCTCCGTCTCAACCCATCACGTTGTCTTCATTGACTGCTGAGTTTTCCCTCGAAGTGGAAGCACGAAAATGTTATCAATGTCGTGAAAGAAAAGGTGAATGCCTGGTTGAATATGGAAATTATAAATGCGCCGCCAGAGAAAAAGGTATATACATTTACATGACCTGTTTTTTCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

249

Amino Acids

27.4

Weight (kDa)

5.03

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AciI CCGC 8 cut(s) 77, 171, 304, 465, 492, 524, 527, 696
AclWI GGATC 3 cut(s) 152, 208, 324
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 2 cut(s) 245, 436
AdeI CACNNNGTG 1 cut(s) 46
AfaI GTAC 1 cut(s) 381
AfeI AGCGCT 1 cut(s) 361
AfiI CCNNNNNNNGG 3 cut(s) 170, 530, 619
AgsI TTSAA 5 cut(s) 145, 223, 377, 412, 674
AjnI CCWGG 3 cut(s) 97, 529, 666
AluBI AGCT 1 cut(s) 135
AluI AGCT 1 cut(s) 135
Alw26I GTCTC 3 cut(s) 285, 570, 576
AlwI GGATC 3 cut(s) 152, 208, 324
Ama87I CYCGRG 1 cut(s) 151
Aor51HI AGCGCT 1 cut(s) 361
AoxI GGCC 2 cut(s) 100, 206
ApeKI GCWGC 2 cut(s) 321, 462
ApoI RAATTY 2 cut(s) 245, 436
AspLEI GCGC 2 cut(s) 362, 695
AspS9I GGNCC 1 cut(s) 550
AsuC2I CCSGG 2 cut(s) 152, 153
AsuHPI GGTGA 3 cut(s) 32, 258, 671
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 1 cut(s) 550
BbsI GAAGAC 2 cut(s) 344, 582
BbvI GCAGC 2 cut(s) 308, 449
BccI CCATC 2 cut(s) 323, 587
BciT130I CCWGG 3 cut(s) 99, 531, 668
BciVI GTATCC 1 cut(s) 227
BcnI CCSGG 2 cut(s) 152, 153
BcoDI GTCTC 3 cut(s) 285, 570, 576
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 1 cut(s) 363
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 4 cut(s) 322, 463, 527, 696
BlsI GCNGC 4 cut(s) 323, 464, 528, 697
Bme1390I CCNGG 5 cut(s) 99, 152, 153, 531, 668
Bme18I GGWCC 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 2 cut(s) 81, 552
BmrFI CCNGG 5 cut(s) 99, 152, 153, 531, 668
BoxI GACNNNNGTC 1 cut(s) 348
BpiI GAAGAC 2 cut(s) 344, 582
BpuEI CTTGAG 1 cut(s) 367
BpuMI CCSGG 2 cut(s) 152, 153
BsaBI GATNNNNATC 1 cut(s) 278
BsaI GGTCTC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 151, 250, 530
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 530, 619
Bse1I ACTGG 1 cut(s) 242
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 3 cut(s) 99, 531, 668
BseDI CCNNGG 3 cut(s) 151, 250, 530
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 3 cut(s) 170, 530, 619
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 308, 449
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 38
Bsh1285I CGRYCG 2 cut(s) 205, 304
BshFI GGCC 2 cut(s) 102, 208
BsiEI CGRYCG 2 cut(s) 205, 304
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 3 cut(s) 170, 530, 619
BsmAI GTCTC 3 cut(s) 285, 570, 576
BsmBI CGTCTC 2 cut(s) 570, 576
BsmFI GGGAC 1 cut(s) 536
BsmI GAATGC 2 cut(s) 173, 668
BsnI GGCC 2 cut(s) 102, 208
Bso31I GGTCTC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 151
Bsp143I GATC 5 cut(s) 157, 202, 213, 316, 397
BspACI CCGC 8 cut(s) 77, 171, 304, 465, 492, 524, 527, 696
BspANI GGCC 2 cut(s) 102, 208
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 81, 552
BspMAI CTGCAG 1 cut(s) 113
BspPI GGATC 3 cut(s) 152, 208, 324
BspTNI GGTCTC 1 cut(s) 285
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 151, 250, 530
BssMI GATC 5 cut(s) 157, 202, 213, 316, 397
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 99, 531, 668
Bst4CI ACNGT 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 3 cut(s) 307, 427, 603
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 2 cut(s) 362, 695
BstKTI GATC 5 cut(s) 160, 205, 216, 319, 400
BstMAI GTCTC 3 cut(s) 285, 570, 576
BstMBI GATC 5 cut(s) 157, 202, 213, 316, 397
BstMCI CGRYCG 2 cut(s) 205, 304
BstMWI GCNNNNNNNGC 3 cut(s) 11, 108, 132
BstNI CCWGG 3 cut(s) 99, 531, 668
BstPAI GACNNNNGTC 1 cut(s) 348
BstSCI CCNGG 5 cut(s) 97, 150, 151, 529, 666
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 308, 449
BstV2I GAAGAC 2 cut(s) 344, 582
BstX2I RGATCY 2 cut(s) 157, 213
BstYI RGATCY 2 cut(s) 157, 213
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 2 cut(s) 102, 208
BtgZI GCGATG 1 cut(s) 339
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 124
CfoI GCGC 2 cut(s) 362, 695
Cfr13I GGNCC 1 cut(s) 550
Cfr9I CCCGGG 1 cut(s) 151
Csp6I GTAC 1 cut(s) 380
CviAII CATG 2 cut(s) 242, 724
CviJI RGCY 8 cut(s) 5, 14, 102, 135, 150, 208, 255, 462
CviKI_1 RGCY 8 cut(s) 5, 14, 102, 135, 150, 208, 255, 462
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 3 cut(s) 307, 427, 603
DpnI GATC 5 cut(s) 159, 204, 215, 318, 399
DpnII GATC 5 cut(s) 157, 202, 213, 316, 397
DraIII CACNNNGTG 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 550
Eco47III AGCGCT 1 cut(s) 361
Eco88I CYCGRG 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 550
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 97, 529, 666
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
Esp3I CGTCTC 2 cut(s) 570, 576
FaeI CATG 2 cut(s) 245, 727
FaqI GGGAC 1 cut(s) 536
FatI CATG 2 cut(s) 241, 723
FauI CCCGC 1 cut(s) 531
Fnu4HI GCNGC 4 cut(s) 322, 463, 527, 696
Fsp4HI GCNGC 4 cut(s) 322, 463, 527, 696
GlaI GCGC 2 cut(s) 361, 694
GluI GCNGC 4 cut(s) 322, 463, 527, 696
GsaI CCCAGC 1 cut(s) 360
HaeII RGCGCY 1 cut(s) 363
HaeIII GGCC 2 cut(s) 102, 208
HapII CCGG 1 cut(s) 152
HhaI GCGC 2 cut(s) 362, 695
Hin1II CATG 2 cut(s) 245, 727
Hin6I GCGC 2 cut(s) 360, 693
HinP1I GCGC 2 cut(s) 360, 693
HinfI GANTC 1 cut(s) 349
HpaII CCGG 1 cut(s) 152
HphI GGTGA 3 cut(s) 32, 258, 671
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 4 cut(s) 73, 196, 402, 746
Hpy188III TCNNGA 2 cut(s) 164, 647
Hpy8I GTNNAC 1 cut(s) 180
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 2 cut(s) 151, 279
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 4 cut(s) 55, 111, 417, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 108, 132
HpyF3I CTNAG 3 cut(s) 307, 427, 603
HpySE526I ACGT 1 cut(s) 584
Hsp92II CATG 2 cut(s) 245, 727
HspAI GCGC 2 cut(s) 360, 693
Kzo9I GATC 5 cut(s) 157, 202, 213, 316, 397
Lsp1109I GCAGC 2 cut(s) 308, 449
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 1 cut(s) 113
MalI GATC 5 cut(s) 159, 204, 215, 318, 399
MboI GATC 5 cut(s) 157, 202, 213, 316, 397
MboII GAAGA 2 cut(s) 344, 582
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 2 cut(s) 157, 213
MluCI AATT 5 cut(s) 218, 245, 436, 454, 682
MlyI GAGTC 1 cut(s) 358
MnlI CCTC 4 cut(s) 94, 171, 220, 623
MseI TTAA 2 cut(s) 453, 512
MslI CAYNNNNRTG 2 cut(s) 631, 722
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 5 cut(s) 99, 152, 153, 531, 668
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 2 cut(s) 173, 668
MvaI CCWGG 3 cut(s) 99, 531, 668
MwoI GCNNNNNNNGC 3 cut(s) 11, 108, 132
NciI CCSGG 2 cut(s) 152, 153
NdeII GATC 5 cut(s) 157, 202, 213, 316, 397
NlaIII CATG 2 cut(s) 245, 727
NlaIV GGNNCC 2 cut(s) 81, 552
NmuCI GTSAC 1 cut(s) 113
PctI GAATGC 2 cut(s) 173, 668
PkrI GCNGC 4 cut(s) 323, 464, 528, 697
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 1 cut(s) 357
PpsI GAGTC 1 cut(s) 357
PpuMI RGGWCCY 1 cut(s) 550
PshAI GACNNNNGTC 1 cut(s) 348
PsiI TTATAA 1 cut(s) 687
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 3 cut(s) 97, 529, 666
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 3 cut(s) 97, 529, 666
PspN4I GGNNCC 2 cut(s) 81, 552
PspPI GGNCC 1 cut(s) 550
PspPPI RGGWCCY 1 cut(s) 550
PstI CTGCAG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 157, 213
PvuI CGATCG 1 cut(s) 205
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
RseI CAYNNNNRTG 2 cut(s) 631, 722
SaqAI TTAA 2 cut(s) 453, 512
SatI GCNGC 4 cut(s) 322, 463, 527, 696
Sau3AI GATC 5 cut(s) 157, 202, 213, 316, 397
Sau96I GGNCC 1 cut(s) 550
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 5 cut(s) 99, 152, 153, 531, 668
SfcI CTRYAG 1 cut(s) 109
SinI GGWCC 1 cut(s) 550
SmaI CCCGGG 1 cut(s) 153
SmiMI CAYNNNNRTG 2 cut(s) 631, 722
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 5 cut(s) 218, 245, 436, 454, 682
SsiI CCGC 8 cut(s) 77, 171, 304, 465, 492, 524, 527, 696
SspI AATATT 1 cut(s) 68
StyD4I CCNGG 5 cut(s) 97, 150, 151, 529, 666
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 587
TaqI TCGA 3 cut(s) 272, 339, 615
TasI AATT 5 cut(s) 218, 245, 436, 454, 682
TauI GCSGC 2 cut(s) 529, 698
Tru1I TTAA 2 cut(s) 453, 512
Tru9I TTAA 2 cut(s) 453, 512
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 2 cut(s) 321, 462
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 4 cut(s) 258, 486, 582, 727
TspGWI ACGGA 3 cut(s) 19, 552, 558
TspMI CCCGGG 1 cut(s) 151
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 2 cut(s) 245, 436
XmaI CCCGGG 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.