Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001088

Physical Location & Seq

Genomic Coordinates Reverse (-)

4947494 .. 4954012

Gene Structure 6519 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1051700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGCTGAGCGTGCTACGCGTGCACCTCCCTTCGGATATTCCCATTGTAGGCTGCGAGCTCACGCCATACGTGCTCCTGCGCCGGCCTGACAAGAATGTCACGACAGACGATGTGCCGGAGTCAGCCCCTCTTGATGGCCACTTCTTGAGGTACAAGTGGTATCGTGTACAAAGTGATAAAAAAGTTGCTATCTGTAGTGTACATCCATCTGAGCAAGCCACATTGCAGTGTCTAGGTTGCGTTAAGGCCAAAATACCTGTTGCCAAAAGTTACCATTGTTCACCTAAGTGCTTCTCAGATGCATGGCAGCATCACCGTGTTTTGCATGACCGTGCTGCTACTGCTGTAAATGAAAATGGAAATGAGGAAGAAGAGGTGTTTGGACGCTTCAATAGCTCTGGATCTGGAGTCCTTAACACTAGCTTATCTGGTTCCGTGTCAAGCATGAGTTTGACGAATGGTTCAGCACCTTTATATCCTGCAGCTGTTACACAAAGGAGTGGTGGTGAAACATGGTTTGAAGTGGGCCGCTCTAAAACGTATACACCAACGGCTGATGATATTGGGCATGTTCTTAAGTTTGAATGCGTTGTAGTTGATGCTGAATCAAAAGTACCTGTGGGACATGTCAATACCATATTAACTTCCCGTGTCATTCCAGCACCCTCTCCCAGTCCACGGCGTCTAGTTCCTGTCAATGGAGTTGACGTCATGGGGCATTTAGATTCAGATGGCCGTATTTCATCTTCTGGAACTTTTACTGTGCTTTCATACAACATTTTGTCTGATGTGTATGCCACCAGTGAATCATACAGTTACTGCCCTTCATGGGCACTTTCGTGGCCCTACCGCAGGCAAAATTTGCTGCGGGAAATTGTTGGCTATCGCGCAGATATTGTTTGTCTTCAGGAGGTTCAAAGTGATCATTTCGAGGAATTTTTTGCCCCCGAGCTGGACAAACATGGTTATCAAGCACTATACAAGAGAAAAACAAATGAGGTTTACAATGGAAACACACAAACAATTGATGGTTGTGCAACATTTTTCCGTAGAGATAGATTTTCCCATGTCAAAAAATATGAGGTTGAATTTAATAAGGCTGCTCAGTCTTTGACTGATGCAATGATTCCAAGTAATCAGAAGAAAAGTGCTTTAAATCGATTAGTTAAGGATAATGTTGCGCTCATTGTGGTTCTGGAAGCAAAATTCAGCAATCAGGGAGCTGATAATCCTGGGAAACGGCAACTTCTTTGTGTGGCAAATACGCATGTAAATGTCCACCAAGATCTGAAGGATGTCAAACTCTGGCAGGTTCATACTCTTTTAAAAGGACTAGAGAAAATAGCTGCCAGTGCAGATATCCCGATGCTGGTGTGTGGGGATTTTAATTCAGTTCCTGGAAGCGCTCCTCATACACTTCTGGCTATGGGAAAGGTAGATCCATTGCATACAGATTTACAAGTGGATCCTCTTAATATACTGCGCCCTCTCAGTAAGCTGATACATCAGCTACCACTGGTTAGTGCATATTCATCATTTGCAAGATTGGGAGTTGGTCTTGGGATGGAGCAGCTGAGAAGGAGAATGGATCCCACCACAAATGAGCCGTTATTTACAAATTGTACTAGAGATTTCATTGGTACCCTAGATTACATATTCTACACCGCTGACTCCTTAACGGTGGAGTCCTTGTTGGAACTCTTGGATGAGGAAAGCTTAAGGAAAGACACAGCCCTTCCTTCTCCAGAGTGGTCATCAGATCATATTGCACTTTTAGCCGAGTTTCGCTGCAAGCCTAGAGTTAGACGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000932 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0005986 GO:0006073 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009311 GO:0009312 GO:0009987 GO:0010467 GO:0016051 GO:0016070 GO:0016071 GO:0019252 GO:0032991 GO:0033692 GO:0034637 GO:0034641 GO:0034645 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

Exo_endo_phos zf-C6H2

Protein Analysis

604

Amino Acids

67.09

Weight (kDa)

6.7

Isoelectric Point (pI)

41.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C6H2	PF15801	72 - 110	2.7e-06	zf-MYND-like zinc finger, mRNA-binding
Exo_endo_phos	PF03372	256 - 588	4.6e-26	Endonuclease/Exonuclease/phosphatase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001088)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58560 AT3G58580
fragaria_vesca	FvH4_5g37360 FvH4_5g37360 FvH4_5g37650 FvH4_6g21630 FvH4_6g21630 FvH4_6g21630 FvH4_6g21630 FvH4_6g21630
malus_domestica	MD08G1182500.v1.1 MD12G1053300.v1.1 MD14G1051700.v1.1 MD15G1368200.v1.1 MD15G1368500.v1.1
prunus_persica	Prupe.1G516400_v2.0.a1 Prupe.1G516400_v2.0.a1 Prupe.1G516400_v2.0.a1 Prupe.7G084300_v2.0.a1
pyrus_communis	pycom08g15630 pycom15g33010 pycom15g33030
rosa_chinensis	RchiOBHm_Chr3g0478881 RchiOBHm_Chr7g0227401 RchiOBHm_Chr7g0239031
rosa_laevigata	RLG00000000789 RLG00000001657 RLG00000023618
rosa_multiflora	Rmu_sc0000868.1_g000004 Rmu_sc0004831.1_g000003 Rmu_sc0005651.1_g000003 Rmu_sc0005751.1_g000015
rosa_roxburghii	Rroxscaffold_3G00221350 Rroxscaffold_3G00237900 Rroxscaffold_3G00237980 Rroxscaffold_3G00238050 Rroxscaffold_5G00332920 Rroxscaffold_6G00403000 Rroxscaffold_6G00413120 Rroxscaffold_7G00214960
rosa_rugosa	Rorug03G0168800 Rorug03G0168900 Rorug07G0324800
rosa_samantha	Rh2DG401300 Rh3AG220500 Rh3BG252400 Rh3CG249200 Rh3DG245400 Rh6AG177800 Rh7AG393200 Rh7AG479600 Rh7BG452100 Rh7CG412400 Rh7CG497100 Rh7DG464600
rosa_wichuraiana	Rw2G030830 Rw3G019830 Rw6G015170 Rw7G040100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	95
AatII	GACGTC	1 cut(s)	711
Acc36I	ACCTGC	1 cut(s)	1300
Acc65I	GGTACC	1 cut(s)	1640
AccB1I	GGYRCC	1 cut(s)	1640
AccBSI	CCGCTC	1 cut(s)	531
AccI	GTMKAC	1 cut(s)	542
AccII	CGCG	2 cut(s)	18, 888
AciI	CCGC	4 cut(s)	529, 850, 868, 1665
AclWI	GGATC	6 cut(s)	409, 1433, 1460, 1473, 1583, 1596
AcoI	YGGCCR	2 cut(s)	136, 733
AcsI	RAATTY	4 cut(s)	859, 935, 1088, 1205
AcuI	CTGAAG	2 cut(s)	890, 1310
AcyI	GRCGYC	2 cut(s)	682, 708
AfaI	GTAC	6 cut(s)	152, 168, 201, 615, 1624, 1642
AfeI	AGCGCT	1 cut(s)	1405
AfiI	CCNNNNNNNGG	9 cut(s)	31, 47, 134, 678, 698, 829, 852, 952, 1369
AflII	CTTAAG	2 cut(s)	575, 1717
AflIII	ACRYGT	2 cut(s)	16, 625
AgsI	TTSAA	5 cut(s)	391, 521, 584, 917, 1088
AjnI	CCWGG	2 cut(s)	1231, 1396
AjuI	GAANNNNNNNTTGG	2 cut(s)	363, 395
AleI	CACNNNNGTG	2 cut(s)	286, 838
Alw21I	GWGCWC	3 cut(s)	24, 60, 75
Alw44I	GTGCAC	1 cut(s)	20
AlwI	GGATC	6 cut(s)	409, 1433, 1460, 1473, 1583, 1596
AlwNI	CAGNNNCTG	2 cut(s)	819, 1397
Ama87I	CYCGRG	1 cut(s)	947
Aor51HI	AGCGCT	1 cut(s)	1405
AoxI	GGCC	6 cut(s)	83, 136, 246, 526, 733, 842
ApaLI	GTGCAC	1 cut(s)	20
ApeKI	GCWGC	9 cut(s)	51, 307, 335, 482, 865, 1100, 1346, 1570, 1788
ApoI	RAATTY	4 cut(s)	859, 935, 1088, 1205
Asp718I	GGTACC	1 cut(s)	1640
AspLEI	GCGC	5 cut(s)	81, 890, 1183, 1406, 1485
AspS9I	GGNCC	2 cut(s)	526, 843
AsuHPI	GGTGA	3 cut(s)	273, 305, 518
AvaI	CYCGRG	1 cut(s)	947
BaeGI	GKGCMC	2 cut(s)	24, 835
BalI	TGGCCA	1 cut(s)	138
BamHI	GGATCC	2 cut(s)	1465, 1588
BanI	GGYRCC	1 cut(s)	1640
BanII	GRGCYC	1 cut(s)	60
BarI	GAAGNNNNNNTAC	2 cut(s)	730, 762
BbsI	GAAGAC	1 cut(s)	896
Bbv12I	GWGCWC	3 cut(s)	24, 60, 75
BbvI	GCAGC	9 cut(s)	38, 319, 322, 494, 852, 1087, 1333, 1582, 1775
BccI	CCATC	5 cut(s)	128, 214, 725, 1022, 1558
BceAI	ACGGC	5 cut(s)	567, 695, 720, 1256, 1591
BciT130I	CCWGG	2 cut(s)	1233, 1398
BclI	TGATCA	1 cut(s)	922
BfaI	CTAG	7 cut(s)	233, 420, 686, 1334, 1626, 1646, 1797
BfmI	CTRYAG	2 cut(s)	193, 480
BfoI	RGCGCY	1 cut(s)	1407
BfrI	CTTAAG	2 cut(s)	575, 1717
BfuAI	ACCTGC	1 cut(s)	1300
BglII	AGATCT	1 cut(s)	1285
BlpI	GCTNAGC	1 cut(s)	5
Bme1390I	CCNGG	2 cut(s)	1233, 1398
BmeT110I	CYCGRG	1 cut(s)	947
BmgT120I	GGNCC	2 cut(s)	526, 843
BmiI	GGNNCC	4 cut(s)	433, 1467, 1590, 1642
BmrFI	CCNGG	2 cut(s)	1233, 1398
BmrI	ACTGGG	1 cut(s)	666
BmsI	GCATC	5 cut(s)	289, 319, 589, 1108, 1356
BmuI	ACTGGG	1 cut(s)	666
BpiI	GAAGAC	1 cut(s)	896
BpmI	CTGGAG	2 cut(s)	426, 1728
Bpu1102I	GCTNAGC	1 cut(s)	5
BpuEI	CTTGAG	1 cut(s)	166
Bsa29I	ATCGAT	1 cut(s)	1159
BsaAI	YACGTR	1 cut(s)	70
BsaHI	GRCGYC	2 cut(s)	682, 708
BsaJI	CCNNGG	2 cut(s)	677, 1232
BsaXI	ACNNNNNCTCC	6 cut(s)	490, 520, 693, 723, 1676, 1706
Bsc4I	CCNNNNNNNGG	9 cut(s)	31, 47, 134, 678, 698, 829, 852, 952, 1369
Bse118I	RCCGGY	1 cut(s)	81
Bse1I	ACTGG	4 cut(s)	672, 801, 1350, 1521
Bse3DI	GCAATG	3 cut(s)	221, 1128, 1442
BseBI	CCWGG	2 cut(s)	1233, 1398
BseCI	ATCGAT	1 cut(s)	1159
BseDI	CCNNGG	2 cut(s)	677, 1232
BseGI	GGATG	4 cut(s)	202, 1300, 1569, 1711
BseLI	CCNNNNNNNGG	9 cut(s)	31, 47, 134, 678, 698, 829, 852, 952, 1369
BseMI	GCAATG	3 cut(s)	221, 1128, 1442
BseMII	CTCAG	5 cut(s)	201, 309, 1118, 1504, 1565
BseNI	ACTGG	4 cut(s)	672, 801, 1350, 1521
BseRI	GAGGAG	1 cut(s)	1398
BseSI	GKGCMC	2 cut(s)	24, 835
BseXI	GCAGC	9 cut(s)	38, 319, 322, 494, 852, 1087, 1333, 1582, 1775
BsgI	GTGCAG	1 cut(s)	1374
Bsh1236I	CGCG	2 cut(s)	18, 888
BshFI	GGCC	6 cut(s)	85, 138, 248, 528, 735, 844
BshNI	GGYRCC	1 cut(s)	1640
BshVI	ATCGAT	1 cut(s)	1159
BsiHKAI	GWGCWC	3 cut(s)	24, 60, 75
BsiHKCI	CYCGRG	1 cut(s)	947
BsiSI	CCGG	2 cut(s)	82, 116
BslFI	GGGAC	1 cut(s)	636
BslI	CCNNNNNNNGG	9 cut(s)	31, 47, 134, 678, 698, 829, 852, 952, 1369
BsmFI	GGGAC	1 cut(s)	636
BsmI	GAATGC	1 cut(s)	590
BsnI	GGCC	6 cut(s)	85, 138, 248, 528, 735, 844
BsoBI	CYCGRG	1 cut(s)	947
Bsp1286I	GDGCHC	4 cut(s)	24, 60, 75, 835
Bsp1407I	TGTACA	2 cut(s)	166, 199
Bsp143I	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
Bsp1720I	GCTNAGC	1 cut(s)	5
BspACI	CCGC	4 cut(s)	529, 850, 868, 1665
BspANI	GGCC	6 cut(s)	85, 138, 248, 528, 735, 844
BspCNI	CTCAG	5 cut(s)	202, 308, 1117, 1503, 1566
BspDI	ATCGAT	1 cut(s)	1159
BspFNI	CGCG	2 cut(s)	18, 888
BspLI	GGNNCC	4 cut(s)	433, 1467, 1590, 1642
BspMAI	CTGCAG	1 cut(s)	484
BspMI	ACCTGC	1 cut(s)	1300
BspPI	GGATC	6 cut(s)	409, 1433, 1460, 1473, 1583, 1596
BspT107I	GGYRCC	1 cut(s)	1640
BspTI	CTTAAG	2 cut(s)	575, 1717
BsrBI	CCGCTC	1 cut(s)	531
BsrDI	GCAATG	3 cut(s)	221, 1128, 1442
BsrFI	RCCGGY	1 cut(s)	81
BsrGI	TGTACA	2 cut(s)	166, 199
BsrI	ACTGG	4 cut(s)	672, 801, 1350, 1521
BssAI	RCCGGY	1 cut(s)	81
BssECI	CCNNGG	2 cut(s)	677, 1232
BssMI	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
BssNAI	GTATAC	1 cut(s)	543
BssNI	GRCGYC	2 cut(s)	682, 708
Bst1107I	GTATAC	1 cut(s)	543
Bst2UI	CCWGG	2 cut(s)	1233, 1398
Bst4CI	ACNGT	5 cut(s)	317, 332, 763, 815, 1681
Bst6I	CTCTTC	1 cut(s)	366
BstACI	GRCGYC	2 cut(s)	682, 708
BstAFI	CTTAAG	2 cut(s)	575, 1717
BstAPI	GCANNNNNTGC	1 cut(s)	862
BstAUI	TGTACA	2 cut(s)	166, 199
BstBAI	YACGTR	1 cut(s)	70
BstC8I	GCNNGC	7 cut(s)	11, 20, 56, 83, 216, 854, 1793
BstDEI	CTNAG	7 cut(s)	5, 210, 285, 295, 1104, 1490, 1574
BstDSI	CCRYGG	1 cut(s)	677
BstENI	CCTNNNNNAGG	1 cut(s)	850
BstF5I	GGATG	4 cut(s)	202, 1300, 1569, 1711
BstFNI	CGCG	2 cut(s)	18, 888
BstH2I	RGCGCY	1 cut(s)	1407
BstHHI	GCGC	5 cut(s)	81, 890, 1183, 1406, 1485
BstKTI	GATC	7 cut(s)	404, 925, 1288, 1441, 1468, 1591, 1762
BstMBI	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
BstMWI	GCNNNNNNNGC	9 cut(s)	10, 15, 19, 70, 341, 393, 862, 1352, 1775
BstNI	CCWGG	2 cut(s)	1233, 1398
BstNSI	RCATGY	3 cut(s)	572, 629, 1271
BstSCI	CCNGG	2 cut(s)	1231, 1396
BstSFI	CTRYAG	2 cut(s)	193, 480
BstSLI	GKGCMC	2 cut(s)	24, 835
BstUI	CGCG	2 cut(s)	18, 888
BstV1I	GCAGC	9 cut(s)	38, 319, 322, 494, 852, 1087, 1333, 1582, 1775
BstV2I	GAAGAC	1 cut(s)	896
BstX2I	RGATCY	5 cut(s)	401, 1285, 1438, 1465, 1588
BstYI	RGATCY	5 cut(s)	401, 1285, 1438, 1465, 1588
BstZ17I	GTATAC	1 cut(s)	543
Bsu15I	ATCGAT	1 cut(s)	1159
BsuRI	GGCC	6 cut(s)	85, 138, 248, 528, 735, 844
BsuTUI	ATCGAT	1 cut(s)	1159
BtgI	CCRYGG	1 cut(s)	677
BtsCI	GGATG	4 cut(s)	202, 1300, 1569, 1711
BtsI	GCAGTG	1 cut(s)	233
BtsIMutI	CAGTG	4 cut(s)	233, 808, 1357, 1514
BveI	ACCTGC	1 cut(s)	1300
Cac8I	GCNNGC	7 cut(s)	11, 20, 56, 83, 216, 854, 1793
CaiI	CAGNNNCTG	2 cut(s)	819, 1397
CfoI	GCGC	5 cut(s)	81, 890, 1183, 1406, 1485
Cfr10I	RCCGGY	1 cut(s)	81
Cfr13I	GGNCC	2 cut(s)	526, 843
ClaI	ATCGAT	1 cut(s)	1159
CseI	GACGC	2 cut(s)	393, 671
Csp6I	GTAC	6 cut(s)	151, 167, 200, 614, 1623, 1641
CviQI	GTAC	6 cut(s)	151, 167, 200, 614, 1623, 1641
DdeI	CTNAG	7 cut(s)	5, 210, 285, 295, 1104, 1490, 1574
DpnI	GATC	7 cut(s)	403, 924, 1287, 1440, 1467, 1590, 1761
DpnII	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
DraI	TTTAAA	2 cut(s)	1155, 1326
DrdI	GACNNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	1 cut(s)	95
EaeI	YGGCCR	2 cut(s)	136, 733
Eam1104I	CTCTTC	1 cut(s)	366
EarI	CTCTTC	1 cut(s)	366
Ecl136II	GAGCTC	1 cut(s)	58
Eco24I	GRGCYC	1 cut(s)	60
Eco32I	GATATC	1 cut(s)	1360
Eco47III	AGCGCT	1 cut(s)	1405
Eco53kI	GAGCTC	1 cut(s)	58
Eco57I	CTGAAG	2 cut(s)	890, 1310
Eco88I	CYCGRG	1 cut(s)	947
EcoICRI	GAGCTC	1 cut(s)	58
EcoNI	CCTNNNNNAGG	1 cut(s)	850
EcoRII	CCWGG	2 cut(s)	1231, 1396
EcoRV	GATATC	1 cut(s)	1360
EcoT22I	ATGCAT	1 cut(s)	304
EcoT38I	GRGCYC	1 cut(s)	60
FaqI	GGGAC	1 cut(s)	636
FauI	CCCGC	1 cut(s)	861
FbaI	TGATCA	1 cut(s)	922
FblI	GTMKAC	1 cut(s)	542
FokI	GGATG	4 cut(s)	189, 1307, 1576, 1718
FriOI	GRGCYC	1 cut(s)	60
FspBI	CTAG	7 cut(s)	233, 420, 686, 1334, 1626, 1646, 1797
GlaI	GCGC	5 cut(s)	80, 889, 1182, 1405, 1484
GsuI	CTGGAG	2 cut(s)	426, 1728
HaeII	RGCGCY	1 cut(s)	1407
HaeIII	GGCC	6 cut(s)	85, 138, 248, 528, 735, 844
HapII	CCGG	2 cut(s)	82, 116
HgaI	GACGC	2 cut(s)	393, 671
HhaI	GCGC	5 cut(s)	81, 890, 1183, 1406, 1485
Hin1I	GRCGYC	2 cut(s)	682, 708
Hin6I	GCGC	5 cut(s)	79, 888, 1181, 1404, 1483
HinP1I	GCGC	5 cut(s)	79, 888, 1181, 1404, 1483
HincII	GTYRAC	1 cut(s)	706
HindII	GTYRAC	1 cut(s)	706
HindIII	AAGCTT	1 cut(s)	1714
HinfI	GANTC	8 cut(s)	119, 408, 605, 725, 806, 1126, 1670, 1685
HpaII	CCGG	2 cut(s)	82, 116
HphI	GGTGA	3 cut(s)	273, 305, 518
Hpy166II	GTNNAC	9 cut(s)	22, 167, 200, 281, 543, 677, 706, 1003, 1279
Hpy188I	TCNGA	8 cut(s)	34, 211, 298, 730, 787, 1140, 1290, 1759
Hpy8I	GTNNAC	9 cut(s)	22, 167, 200, 281, 543, 677, 706, 1003, 1279
HpyAV	CCTTC	6 cut(s)	39, 834, 1285, 1572, 1745, 1749
HpyCH4III	ACNGT	5 cut(s)	317, 332, 763, 815, 1681
HpyCH4IV	ACGT	4 cut(s)	69, 539, 708, 1807
HpyF10VI	GCNNNNNNNGC	9 cut(s)	10, 15, 19, 70, 341, 393, 862, 1352, 1775
HpyF3I	CTNAG	7 cut(s)	5, 210, 285, 295, 1104, 1490, 1574
HpySE526I	ACGT	4 cut(s)	69, 539, 708, 1807
Hsp92I	GRCGYC	2 cut(s)	682, 708
HspAI	GCGC	5 cut(s)	79, 888, 1181, 1404, 1483
KpnI	GGTACC	1 cut(s)	1644
KroI	GCCGGC	1 cut(s)	81
KroNI	GCCGGC	1 cut(s)	83
Ksp22I	TGATCA	1 cut(s)	922
Kzo9I	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
LmnI	GCTCC	4 cut(s)	78, 1220, 1411, 1567
Lsp1109I	GCAGC	9 cut(s)	38, 319, 322, 494, 852, 1087, 1333, 1582, 1775
LweI	GCATC	5 cut(s)	289, 319, 589, 1108, 1356
MaeI	CTAG	7 cut(s)	233, 420, 686, 1334, 1626, 1646, 1797
MaeII	ACGT	4 cut(s)	69, 539, 708, 1807
MaeIII	GTNAC	4 cut(s)	97, 269, 487, 815
MalI	GATC	7 cut(s)	403, 924, 1287, 1440, 1467, 1590, 1761
MbiI	CCGCTC	1 cut(s)	531
MboI	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
MboII	GAAGA	5 cut(s)	380, 383, 738, 896, 1153
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	5 cut(s)	401, 1285, 1438, 1465, 1588
MhlI	GDGCHC	4 cut(s)	24, 60, 75, 835
MlsI	TGGCCA	1 cut(s)	138
MluCI	AATT	8 cut(s)	859, 873, 935, 1023, 1088, 1205, 1387, 1618
MluI	ACGCGT	1 cut(s)	16
MluNI	TGGCCA	1 cut(s)	138
MlyI	GAGTC	4 cut(s)	128, 417, 1664, 1694
MmeI	TCCRAC	1 cut(s)	1674
Mox20I	TGGCCA	1 cut(s)	138
Mph1103I	ATGCAT	1 cut(s)	304
MroNI	GCCGGC	1 cut(s)	81
MscI	TGGCCA	1 cut(s)	138
MslI	CAYNNNNRTG	6 cut(s)	226, 286, 315, 330, 838, 1272
Msp20I	TGGCCA	1 cut(s)	138
MspA1I	CMGCKG	3 cut(s)	485, 1573, 1667
MspCI	CTTAAG	2 cut(s)	575, 1717
MspI	CCGG	2 cut(s)	82, 116
MspR9I	CCNGG	2 cut(s)	1233, 1398
MunI	CAATTG	1 cut(s)	1023
Mva1269I	GAATGC	1 cut(s)	590
MvaI	CCWGG	2 cut(s)	1233, 1398
MvnI	CGCG	2 cut(s)	18, 888
MwoI	GCNNNNNNNGC	9 cut(s)	10, 15, 19, 70, 341, 393, 862, 1352, 1775
NaeI	GCCGGC	1 cut(s)	83
NdeII	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
NgoMIV	GCCGGC	1 cut(s)	81
NlaIV	GGNNCC	4 cut(s)	433, 1467, 1590, 1642
NmeAIII	GCCGAG	1 cut(s)	1804
NmuCI	GTSAC	1 cut(s)	97
NsiI	ATGCAT	1 cut(s)	304
NspI	RCATGY	3 cut(s)	572, 629, 1271
OliI	CACNNNNGTG	2 cut(s)	286, 838
PciI	ACATGT	1 cut(s)	625
PctI	GAATGC	1 cut(s)	590
PdiI	GCCGGC	1 cut(s)	83
PfeI	GAWTC	4 cut(s)	605, 725, 806, 1126
PfoI	TCCNGGA	1 cut(s)	1396
PleI	GAGTC	4 cut(s)	127, 416, 1664, 1693
PpsI	GAGTC	4 cut(s)	127, 416, 1664, 1693
Ppu21I	YACGTR	1 cut(s)	70
PscI	ACATGT	1 cut(s)	625
Psp124BI	GAGCTC	1 cut(s)	60
Psp6I	CCWGG	2 cut(s)	1231, 1396
PspGI	CCWGG	2 cut(s)	1231, 1396
PspN4I	GGNNCC	4 cut(s)	433, 1467, 1590, 1642
PspPI	GGNCC	2 cut(s)	526, 843
PstI	CTGCAG	1 cut(s)	484
PstNI	CAGNNNCTG	2 cut(s)	819, 1397
PsuI	RGATCY	5 cut(s)	401, 1285, 1438, 1465, 1588
PvuII	CAGCTG	2 cut(s)	485, 1573
RsaI	GTAC	6 cut(s)	152, 168, 201, 615, 1624, 1642
RsaNI	GTAC	6 cut(s)	151, 167, 200, 614, 1623, 1641
RseI	CAYNNNNRTG	6 cut(s)	226, 286, 315, 330, 838, 1272
SacI	GAGCTC	1 cut(s)	60
Sau3AI	GATC	7 cut(s)	401, 922, 1285, 1438, 1465, 1588, 1759
Sau96I	GGNCC	2 cut(s)	526, 843
SchI	GAGTC	4 cut(s)	128, 417, 1664, 1694
ScrFI	CCNGG	2 cut(s)	1233, 1398
SduI	GDGCHC	4 cut(s)	24, 60, 75, 835
SfaNI	GCATC	5 cut(s)	289, 319, 589, 1108, 1356
SfcI	CTRYAG	2 cut(s)	193, 480
SmiMI	CAYNNNNRTG	6 cut(s)	226, 286, 315, 330, 838, 1272
SmlI	CTYRAG	3 cut(s)	145, 575, 1717
SmoI	CTYRAG	3 cut(s)	145, 575, 1717
Sse9I	AATT	8 cut(s)	859, 873, 935, 1023, 1088, 1205, 1387, 1618
SsiI	CCGC	4 cut(s)	529, 850, 868, 1665
SspMI	CTAG	7 cut(s)	233, 420, 686, 1334, 1626, 1646, 1797
SstI	GAGCTC	1 cut(s)	60
StyD4I	CCNGG	2 cut(s)	1231, 1396
TaaI	ACNGT	5 cut(s)	317, 332, 763, 815, 1681
TaiI	ACGT	4 cut(s)	72, 542, 711, 1810
TaqI	TCGA	2 cut(s)	930, 1159
TasI	AATT	8 cut(s)	859, 873, 935, 1023, 1088, 1205, 1387, 1618
TatI	WGTACW	3 cut(s)	166, 199, 1622
TauI	GCSGC	1 cut(s)	531
TfiI	GAWTC	4 cut(s)	605, 725, 806, 1126
TscAI	CASTG	4 cut(s)	233, 808, 1357, 1521
TseFI	GTSAC	1 cut(s)	97
TseI	GCWGC	9 cut(s)	51, 307, 335, 482, 865, 1100, 1346, 1570, 1788
Tsp45I	GTSAC	1 cut(s)	97
TspDTI	ATGAA	7 cut(s)	366, 732, 759, 816, 1304, 1521, 1624
TspGWI	ACGGA	2 cut(s)	424, 1037
TspRI	CASTG	4 cut(s)	233, 808, 1357, 1521
Vha464I	CTTAAG	2 cut(s)	575, 1717
VneI	GTGCAC	1 cut(s)	20
XagI	CCTNNNNNAGG	1 cut(s)	850
XapI	RAATTY	4 cut(s)	859, 935, 1088, 1205
XceI	RCATGY	3 cut(s)	572, 629, 1271
XmiI	GTMKAC	1 cut(s)	542
XspI	CTAG	7 cut(s)	233, 420, 686, 1334, 1626, 1646, 1797
ZraI	GACGTC	1 cut(s)	709
Zsp2I	ATGCAT	1 cut(s)	304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.