Rroxscaffold_3G00221350

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001088
Physical Location & Seq
Reverse (-)
4167757 .. 4175805
8049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221350.1

Sequence Viewer

Length: 1446 bp
ATGTGCCATCGTTGTGCTAAAGCTGTTGGTGAGACTTCTTCTGATAATCAACAAGCAGAAGAAGAGATGTATAGCTGGCTTGACTTTGAAGATGATCCACTCAATGAAATTCCAAAGCTGGAACGAGATGGAAAACAATGGGTTGAGGTGTGCTCTTCAAGGTGTTATGTACCCACAGTGAATGACCTTAACTGCAGGTTGATGCTGGAATCTAAGGTTGTTGATTGTTCAACTGGTCTGGATTTGTGCCTGAGTGACATCATCATGACTGTTCCTGTTCTTTATCCGCCTCATCAGAAACCTCGCTCCATGCTTGAGTGTCGGCGGAAGCTTGGGGACTCTGATCTTAAGGCGCACACTTCCAGTGGTGTCAAATTTAGTGTGCTATCATATAATGTCCTTGGTAATCTTTATACTGATCCGTTTAGGTACTCTTACTGCCCAGAATGGGCTAGGTCTTGGGAATATCGTCAGCAGAATTTACTAAACGAGATCATCGAATATGATGCAGACATCCTCTGTCTTCAAGAGGTACAAAGTAATCATCTTGAGTATTTCTTCGAACCTGAGTTGGCAAAATGTGGATATTCTGTTATATACAAGAAGAGAAGAGGATCATTTTTTGCAAGAGACGGGTTTTGCATGGATGGTTGTGCTACATTTTACCGCCGAGATGTATTCAAAGAAATCGTAAAATATGAGTGTGCGTTTGATAGCTCTATTATGAAAGTGATAGAAGCATTGCAGCCATTGGAACCTCAACAGAGAGATAGAGCTTGTATAAGGCTGAAGAAGGACAATGTAGCACTTGTTGTTATATTAGAAAAACTGGAAAGTGAGGGCACTTGCGATGGAATTCAACCTAGAATTTGTGTGGCAAATACGCACATATCTGCAAATCCAAAGTTCCCAGATGTAAAATTGTTCCAGGTTGTGGACCTGATTACTGGACTGGACAAAATTGCCAAGATGAAAATTCCTCTGTTGGTTTGTGGAGATTTCAACTCTCTTCCTCTGAGTGAGCCTCATAAGTTCATAACTGGTGGCAGAGTCGACAATCTTCCCAACGATACTGATCCATTTGGCATATGTCAGCATCTAAAGCTTCACCATTCACTTCCACTGAAAAGTGCATATGCATCTTTTTATAACTCGGATGGTATCGAGGAGGAACAACGTAGGAAGTTCAATTCTGAAAGCAGGGAGCCTTTGTTCACTCACTTCACGCCAAGTTTCACAGGAACCCTAGATTACCTATTCTACACAGAGGATAGTTTGACGCTTGATGGTGTATTGGAGCTACTTGACCGTGACAATCTGGGTGTTGGTATTCCATCTCCTTTTTGGTCATCAGATCATATTGCCCTCATGGCACGGTTTGTCCTCAATTCAACCTCTCCCCAGCAACAGGGTTCACAAGCAATTCCTGCTGACTTGTGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

481

Amino Acids

54.88

Weight (kDa)

5.14

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 129 - 453 2.1e-22 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1149
Acc36I ACCTGC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 934
AccI GTMKAC 1 cut(s) 1053
AciI CCGC 3 cut(s) 287, 325, 667
AclWI GGATC 4 cut(s) 89, 413, 622, 1070
AcoI YGGCCR 1 cut(s) 1439
AcsI RAATTY 6 cut(s) 108, 374, 478, 855, 867, 975
AcuI CTGAAG 1 cut(s) 809
AfaI GTAC 3 cut(s) 171, 431, 534
AfiI CCNNNNNNNGG 3 cut(s) 448, 934, 1408
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 9 cut(s) 89, 159, 231, 527, 682, 860, 1003, 1189, 1392
AjnI CCWGG 1 cut(s) 927
AloI GAACNNNNNNTCC 2 cut(s) 1196, 1228
AluBI AGCT 8 cut(s) 23, 75, 118, 331, 717, 776, 1105, 1300
AluI AGCT 8 cut(s) 23, 75, 118, 331, 717, 776, 1105, 1300
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 2 cut(s) 26, 624
AlwI GGATC 4 cut(s) 89, 413, 622, 1070
AoxI GGCC 1 cut(s) 1439
ApeKI GCWGC 1 cut(s) 745
ApoI RAATTY 6 cut(s) 108, 374, 478, 855, 867, 975
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 1 cut(s) 937
AsuHPI GGTGA 2 cut(s) 41, 1100
AsuII TTCGAA 1 cut(s) 561
AvaII GGWCC 1 cut(s) 937
BaeGI GKGCMC 1 cut(s) 845
BalI TGGCCA 1 cut(s) 1441
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 757
BccI CCATC 7 cut(s) 15, 122, 641, 845, 1151, 1280, 1342
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BciT130I CCWGG 1 cut(s) 929
BcoDI GTCTC 2 cut(s) 26, 624
BfaI CTAG 3 cut(s) 453, 864, 1247
BfmI CTRYAG 1 cut(s) 193
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 186
BglI GCCNNNNNGGC 1 cut(s) 1370
BisI GCNGC 1 cut(s) 746
BlsI GCNGC 1 cut(s) 747
Bme1390I CCNGG 1 cut(s) 929
Bme18I GGWCC 1 cut(s) 937
BmgT120I GGNCC 1 cut(s) 937
BmiI GGNNCC 3 cut(s) 756, 1206, 1243
BmrFI CCNGG 1 cut(s) 929
BmsI GCATC 4 cut(s) 192, 496, 1105, 1148
BpiI GAAGAC 1 cut(s) 515
BplI GAGNNNNNCTC 4 cut(s) 137, 169, 1009, 1041
Bpu14I TTCGAA 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 335, 569
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 1196, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 934, 1408
Bse1I ACTGG 6 cut(s) 238, 363, 834, 952, 957, 1045
Bse3DI GCAATG 1 cut(s) 740
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 1 cut(s) 929
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 3 cut(s) 513, 652, 1162
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 3 cut(s) 448, 934, 1408
BseMI GCAATG 1 cut(s) 740
BseMII CTCAG 3 cut(s) 242, 558, 1007
BseNI ACTGG 6 cut(s) 238, 363, 834, 952, 957, 1045
BseRI GAGGAG 1 cut(s) 1181
BseSI GKGCMC 1 cut(s) 845
BseXI GCAGC 1 cut(s) 757
BseYI CCCAGC 1 cut(s) 1401
BshFI GGCC 1 cut(s) 1441
BsiHKAI GWGCWC 1 cut(s) 155
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 448, 934, 1408
BsmAI GTCTC 2 cut(s) 26, 624
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 1 cut(s) 1441
Bsp119I TTCGAA 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 155, 845
Bsp143I GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
BspACI CCGC 3 cut(s) 287, 325, 667
BspANI GGCC 1 cut(s) 1441
BspCNI CTCAG 3 cut(s) 243, 559, 1008
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 756, 1206, 1243
BspMAI CTGCAG 1 cut(s) 197
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 4 cut(s) 89, 413, 622, 1070
BspQI GCTCTTC 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 561
BspTI CTTAAG 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 740
BsrI ACTGG 6 cut(s) 238, 363, 834, 952, 957, 1045
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
BssT1I CCWWGG 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 929
Bst4CI ACNGT 4 cut(s) 178, 271, 1310, 1377
Bst6I CTCTTC 5 cut(s) 57, 160, 599, 604, 1014
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstBI TTCGAA 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 4 cut(s) 213, 251, 567, 1016
BstF5I GGATG 3 cut(s) 513, 652, 1162
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 7 cut(s) 97, 346, 421, 495, 617, 1078, 1357
BstMAI GTCTC 2 cut(s) 26, 624
BstMBI GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
BstMWI GCNNNNNNNGC 3 cut(s) 1102, 1370, 1427
BstNI CCWGG 1 cut(s) 929
BstSCI CCNGG 1 cut(s) 927
BstSFI CTRYAG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 845
BstV1I GCAGC 1 cut(s) 757
BstV2I GAAGAC 1 cut(s) 515
BsuRI GGCC 1 cut(s) 1441
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 3 cut(s) 513, 652, 1162
BtsIMutI CAGTG 3 cut(s) 183, 370, 1121
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 77
CciI TCATGA 1 cut(s) 264
CfoI GCGC 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 937
CseI GACGC 1 cut(s) 1288
Csp6I GTAC 3 cut(s) 170, 430, 533
CviAII CATG 4 cut(s) 265, 310, 643, 1369
CviQI GTAC 3 cut(s) 170, 430, 533
DdeI CTNAG 4 cut(s) 213, 251, 567, 1016
DpnI GATC 7 cut(s) 96, 345, 420, 494, 616, 1077, 1356
DpnII GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
EaeI YGGCCR 1 cut(s) 1439
Eam1104I CTCTTC 5 cut(s) 57, 160, 599, 604, 1014
EarI CTCTTC 5 cut(s) 57, 160, 599, 604, 1014
EciI GGCGGA 2 cut(s) 276, 340
Eco130I CCWWGG 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 855
EcoRII CCWGG 1 cut(s) 927
EcoT14I CCWWGG 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 1141
ErhI CCWWGG 1 cut(s) 400
Esp3I CGTCTC 1 cut(s) 624
FaeI CATG 4 cut(s) 268, 313, 646, 1372
FaqI GGGAC 1 cut(s) 350
FatI CATG 4 cut(s) 264, 309, 642, 1368
FauNDI CATATG 2 cut(s) 1088, 1135
FblI GTMKAC 1 cut(s) 1053
Fnu4HI GCNGC 1 cut(s) 746
FokI GGATG 3 cut(s) 500, 659, 1169
Fsp4HI GCNGC 1 cut(s) 746
FspBI CTAG 3 cut(s) 453, 864, 1247
GlaI GCGC 1 cut(s) 354
GluI GCNGC 1 cut(s) 746
GsaI CCCAGC 1 cut(s) 1405
HaeIII GGCC 1 cut(s) 1441
HgaI GACGC 1 cut(s) 1288
HhaI GCGC 1 cut(s) 355
Hin1II CATG 4 cut(s) 268, 313, 646, 1372
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 329, 1103
HinfI GANTC 3 cut(s) 209, 338, 1050
HphI GGTGA 2 cut(s) 41, 1100
Hpy166II GTNNAC 4 cut(s) 937, 1054, 1215, 1415
Hpy188I TCNGA 7 cut(s) 43, 297, 343, 1017, 1156, 1195, 1354
Hpy188III TCNNGA 4 cut(s) 239, 265, 527, 548
Hpy8I GTNNAC 4 cut(s) 937, 1054, 1215, 1415
HpyAV CCTTC 1 cut(s) 787
HpyCH4III ACNGT 4 cut(s) 178, 271, 1310, 1377
HpyCH4IV ACGT 1 cut(s) 1177
HpyCH4V TGCA 8 cut(s) 195, 509, 626, 642, 745, 896, 1133, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 1102, 1370, 1427
HpyF3I CTNAG 4 cut(s) 213, 251, 567, 1016
HpySE526I ACGT 1 cut(s) 1177
Hsp92II CATG 4 cut(s) 268, 313, 646, 1372
HspAI GCGC 1 cut(s) 353
Kzo9I GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
LguI GCTCTTC 1 cut(s) 160
LmnI GCTCC 3 cut(s) 311, 1204, 1297
Lsp1109I GCAGC 1 cut(s) 757
LweI GCATC 4 cut(s) 192, 496, 1105, 1148
MaeI CTAG 3 cut(s) 453, 864, 1247
MaeII ACGT 1 cut(s) 1177
MaeIII GTNAC 2 cut(s) 254, 1310
MalI GATC 7 cut(s) 96, 345, 420, 494, 616, 1077, 1356
MboI GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
MhlI GDGCHC 2 cut(s) 155, 845
MlsI TGGCCA 1 cut(s) 1441
MluNI TGGCCA 1 cut(s) 1441
MlyI GAGTC 2 cut(s) 332, 1059
Mox20I TGGCCA 1 cut(s) 1441
Mph1103I ATGCAT 1 cut(s) 1141
MscI TGGCCA 1 cut(s) 1441
MseI TTAA 2 cut(s) 189, 348
MslI CAYNNNNRTG 2 cut(s) 12, 263
Msp20I TGGCCA 1 cut(s) 1441
MspCI CTTAAG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 929
MvaI CCWGG 1 cut(s) 929
MwoI GCNNNNNNNGC 3 cut(s) 1102, 1370, 1427
NdeI CATATG 2 cut(s) 1088, 1135
NdeII GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
NlaIII CATG 4 cut(s) 268, 313, 646, 1372
NlaIV GGNNCC 3 cut(s) 756, 1206, 1243
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 2 cut(s) 254, 1310
NsiI ATGCAT 1 cut(s) 1141
NspV TTCGAA 1 cut(s) 561
PagI TCATGA 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PfeI GAWTC 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 934
PkrI GCNGC 1 cut(s) 747
PleI GAGTC 2 cut(s) 332, 1058
PpsI GAGTC 2 cut(s) 332, 1058
PsiI TTATAA 1 cut(s) 1149
Psp6I CCWGG 1 cut(s) 927
PspFI CCCAGC 1 cut(s) 1401
PspGI CCWGG 1 cut(s) 927
PspN4I GGNNCC 3 cut(s) 756, 1206, 1243
PspPI GGNCC 1 cut(s) 937
PstI CTGCAG 1 cut(s) 197
RsaI GTAC 3 cut(s) 171, 431, 534
RsaNI GTAC 3 cut(s) 170, 430, 533
RseI CAYNNNNRTG 2 cut(s) 12, 263
SalI GTCGAC 1 cut(s) 1052
SapI GCTCTTC 1 cut(s) 160
SaqAI TTAA 2 cut(s) 189, 348
SatI GCNGC 1 cut(s) 746
Sau3AI GATC 7 cut(s) 94, 343, 418, 492, 614, 1075, 1354
Sau96I GGNCC 1 cut(s) 937
SchI GAGTC 2 cut(s) 332, 1059
ScrFI CCNGG 1 cut(s) 929
SduI GDGCHC 2 cut(s) 155, 845
SfaNI GCATC 4 cut(s) 192, 496, 1105, 1148
SfcI CTRYAG 1 cut(s) 193
SfuI TTCGAA 1 cut(s) 561
SinI GGWCC 1 cut(s) 937
SmiMI CAYNNNNRTG 2 cut(s) 12, 263
SmlI CTYRAG 3 cut(s) 314, 347, 548
SmoI CTYRAG 3 cut(s) 314, 347, 548
SsiI CCGC 3 cut(s) 287, 325, 667
SspMI CTAG 3 cut(s) 453, 864, 1247
StyD4I CCNGG 1 cut(s) 927
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 4 cut(s) 178, 271, 1310, 1377
TaiI ACGT 1 cut(s) 1180
TaqI TCGA 4 cut(s) 498, 561, 1053, 1164
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 189, 348
Tru9I TTAA 2 cut(s) 189, 348
TscAI CASTG 3 cut(s) 183, 370, 1128
TseFI GTSAC 2 cut(s) 254, 1310
TseI GCWGC 1 cut(s) 745
Tsp45I GTSAC 2 cut(s) 254, 1310
TspDTI ATGAA 4 cut(s) 120, 740, 986, 1024
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 3 cut(s) 183, 370, 1128
Van91I CCANNNNNTGG 1 cut(s) 934
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 6 cut(s) 108, 374, 478, 855, 867, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 1341
XmiI GTMKAC 1 cut(s) 1053
XspI CTAG 3 cut(s) 453, 864, 1247
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.