pycom08g15630

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001088
Physical Location & Seq
Reverse (-)
15383678 .. 15387440
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15630.1

Sequence Viewer

Length: 1332 bp
ATGGCTCAAGAATCTGGGTCGTCTGAGTCCCGCAATTGGAGACTCAATCTGCGTTTTCCTTCAAGTGCTCCTGTGTTCGGCTGCAGATTCCAGCCCTACATTCATGTGGCCAAACCCAAAGGCTCATTTCAATTCCCGCCTCACGAGAATTCTTTCACCTGGTACCGAGAGCATGTTTGCTCGTCTTCCAATCAAGAGAAGGTTCGCTGTTTTGTCCACCATGACAAGCTGTCCACAATTCAGTGTATAGTTTGTGTAGGTCAAAATCGACATACCAAGATGAGTTACTATTGCTCTGACACTTGTTTTATGGCTGCCTGGAACAAGCACAAGGTGCACCATCATTTTGCAGATCCTGATCCACAAGCAGTGAAAATTATCAAGAGTTTAGGGCCTTATGGCTCCTATCCTGATTCACCTGGCTATCAGGATGACGATTCACAACCTGATGAGGTGGTGGAACAAGATGGGAAAACATGGATTGAAATCGGATCTTGGGAATATTATGTACCCACGGAGCATGATATTGGATTTTGTCTGAGGCTAAAATTTGAAGTTGTTGATTCTTCAACGCGTACACAATTGTGCCCAGCAAGAATCGAAGTGACAGATCCTGTGATTTATCCTCCTCCTCATCCACCGCGTTGTACAATGTGGAACTCTTGCATCGCTGACCTCAAAGAACACTCTTCTAACGATGTAACATTTAGGGTGCTGTCCTATAATATTCTTGCTGATTATCTTGCTACTAGTGGTAGTAACCGGCACAATTACTGCCCAGATTGGGCTCTTTCATGGTCATATCGTCAGCACAATTTACTTTATGAGATCATTGACTATGACGCAGATATCTTATGTCTCCAAGAGGTACAGATAGATCATTTTGAGAATTTCCTCAAACCTGAGTTGGCAAAATATGGATACTTGGTTGTATACAAGAAGAAAAAGACCGAGATATTTACGTTTAAGTCCCAGCTTACAGTTGATGGATGTGCTACATTTTACCGCGATCGACGCTTCAAAGAAATTGCAACATATGAGCTTGAGTTTAATAATTCAGCAAAGCCCTTTGTAGATTCATTGGATGGTGAACAGAAAAATGAAGCTCTCCGTCTGTTGAAGGACAATGTTGCACTTGTTGTTATATTAGAAATGGTGGGAAATGAGAGCTCTTCTGATGGCAACCGACCTAGAATTTGTGTGGTAGTATGTCTATTTCAAATTCTACAGCCTTATATCATAAATCCTCGAGTGCTGACTGCTGACACAGTTGTCCCTTTTGACCATAGGCAAATACCCATATACATGCAAATGTACAAAATCCAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

444

Amino Acids

51.34

Weight (kDa)

6.1

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 239 - 364 6.5e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1271
Acc65I GGTACC 1 cut(s) 162
AccB1I GGYRCC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 933
AccII CGCG 3 cut(s) 574, 643, 1008
AciI CCGC 4 cut(s) 31, 137, 641, 1006
AclWI GGATC 4 cut(s) 347, 353, 499, 605
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 5 cut(s) 148, 548, 889, 1194, 1221
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 6 cut(s) 164, 510, 577, 649, 870, 1316
AfiI CCNNNNNNNGG 3 cut(s) 36, 77, 784
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 8 cut(s) 63, 131, 485, 554, 570, 1021, 1120, 1220
AhdI GACNNNNNGTC 1 cut(s) 229
AhlI ACTAGT 1 cut(s) 749
AjnI CCWGG 3 cut(s) 158, 317, 418
AleI CACNNNNGTG 1 cut(s) 583
AluBI AGCT 5 cut(s) 229, 976, 1042, 1106, 1170
AluI AGCT 5 cut(s) 229, 976, 1042, 1106, 1170
Alw21I GWGCWC 3 cut(s) 70, 339, 1172
Alw26I GTCTC 2 cut(s) 34, 863
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 4 cut(s) 347, 353, 499, 605
AlwNI CAGNNNCTG 2 cut(s) 356, 614
Ama87I CYCGRG 1 cut(s) 1248
AoxI GGCC 2 cut(s) 108, 392
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 81, 314
ApoI RAATTY 5 cut(s) 148, 548, 889, 1194, 1221
ArsI GACNNNNNNTTYG 2 cut(s) 1179, 1211
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 3 cut(s) 148, 408, 1100
AvaI CYCGRG 1 cut(s) 1248
BaeGI GKGCMC 2 cut(s) 339, 590
BalI TGGCCA 1 cut(s) 110
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 2 cut(s) 790, 1172
BauI CACGAG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 3 cut(s) 70, 339, 1172
BbvI GCAGC 2 cut(s) 68, 301
BccI CCATC 5 cut(s) 348, 461, 980, 1079, 1172
BciT130I CCWGG 3 cut(s) 160, 319, 420
BciVI GTATCC 1 cut(s) 914
BcoDI GTCTC 2 cut(s) 34, 863
BcuI ACTAGT 1 cut(s) 749
BfaI CTAG 2 cut(s) 750, 1191
BfmI CTRYAG 2 cut(s) 82, 1226
BfuI GTATCC 1 cut(s) 914
BisI GCNGC 2 cut(s) 82, 315
BlsI GCNGC 2 cut(s) 83, 316
Bme1390I CCNGG 3 cut(s) 160, 319, 420
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 1248
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 2 cut(s) 164, 403
BmrFI CCNGG 3 cut(s) 160, 319, 420
BmsI GCATC 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 1064
BsaBI GATNNNNATC 2 cut(s) 357, 485
BsaJI CCNNGG 1 cut(s) 513
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 77, 784
Bse118I RCCGGY 1 cut(s) 762
Bse8I GATNNNNATC 2 cut(s) 357, 485
BseBI CCWGG 3 cut(s) 160, 319, 420
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 4 cut(s) 436, 634, 995, 1090
BseJI GATNNNNATC 2 cut(s) 357, 485
BseLI CCNNNNNNNGG 3 cut(s) 36, 77, 784
BseMII CTCAG 3 cut(s) 15, 530, 894
BseRI GAGGAG 2 cut(s) 618, 621
BseSI GKGCMC 2 cut(s) 339, 590
BseXI GCAGC 2 cut(s) 68, 301
BseYI CCCAGC 2 cut(s) 589, 972
Bsh1236I CGCG 3 cut(s) 574, 643, 1008
Bsh1285I CGRYCG 1 cut(s) 1012
BshFI GGCC 2 cut(s) 110, 394
BshNI GGYRCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 1012
BsiHKAI GWGCWC 3 cut(s) 70, 339, 1172
BsiHKCI CYCGRG 1 cut(s) 1248
BsiSI CCGG 1 cut(s) 763
BslFI GGGAC 3 cut(s) 13, 955, 1259
BslI CCNNNNNNNGG 3 cut(s) 36, 77, 784
BsmAI GTCTC 2 cut(s) 34, 863
BsmFI GGGAC 3 cut(s) 13, 955, 1259
BsnI GGCC 2 cut(s) 110, 394
BsoBI CYCGRG 1 cut(s) 1248
Bsp1286I GDGCHC 5 cut(s) 70, 339, 590, 790, 1172
Bsp1407I TGTACA 2 cut(s) 647, 1314
Bsp143I GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
BspACI CCGC 4 cut(s) 31, 137, 641, 1006
BspANI GGCC 2 cut(s) 110, 394
BspCNI CTCAG 3 cut(s) 16, 531, 895
BspFNI CGCG 3 cut(s) 574, 643, 1008
BspLI GGNNCC 2 cut(s) 164, 403
BspMAI CTGCAG 1 cut(s) 86
BspPI GGATC 4 cut(s) 347, 353, 499, 605
BspQI GCTCTTC 1 cut(s) 1177
BspT107I GGYRCC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 762
BsrGI TGTACA 2 cut(s) 647, 1314
BssAI RCCGGY 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
BssNAI GTATAC 1 cut(s) 934
BssSI CACGAG 1 cut(s) 143
Bst1107I GTATAC 1 cut(s) 934
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 3 cut(s) 160, 319, 420
Bst4CI ACNGT 2 cut(s) 982, 1270
Bst6I CTCTTC 2 cut(s) 694, 1177
BstAPI GCANNNNNTGC 1 cut(s) 334
BstAUI TGTACA 2 cut(s) 647, 1314
BstDEI CTNAG 3 cut(s) 24, 539, 903
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 4 cut(s) 436, 634, 995, 1090
BstFNI CGCG 3 cut(s) 574, 643, 1008
BstKTI GATC 7 cut(s) 355, 361, 494, 613, 831, 880, 1012
BstMAI GTCTC 2 cut(s) 34, 863
BstMBI GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
BstMCI CGRYCG 1 cut(s) 1012
BstMWI GCNNNNNNNGC 2 cut(s) 334, 1014
BstNI CCWGG 3 cut(s) 160, 319, 420
BstNSI RCATGY 2 cut(s) 176, 1309
BstSCI CCNGG 3 cut(s) 158, 317, 418
BstSFI CTRYAG 2 cut(s) 82, 1226
BstSLI GKGCMC 2 cut(s) 339, 590
BstUI CGCG 3 cut(s) 574, 643, 1008
BstV1I GCAGC 2 cut(s) 68, 301
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 3 cut(s) 352, 491, 610
BstYI RGATCY 3 cut(s) 352, 491, 610
BstZ17I GTATAC 1 cut(s) 934
BsuI GTATCC 1 cut(s) 914
BsuRI GGCC 2 cut(s) 110, 394
BtgI CCRYGG 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 652
BtsCI GGATG 4 cut(s) 436, 634, 995, 1090
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 248, 375
CaiI CAGNNNCTG 2 cut(s) 356, 614
Cfr10I RCCGGY 1 cut(s) 762
Cfr13I GGNCC 1 cut(s) 392
CseI GACGC 2 cut(s) 851, 1023
CsiI ACCWGGT 1 cut(s) 158
Csp6I GTAC 6 cut(s) 163, 509, 576, 648, 869, 1315
CviAII CATG 7 cut(s) 104, 173, 221, 477, 521, 795, 1306
CviQI GTAC 6 cut(s) 163, 509, 576, 648, 869, 1315
DdeI CTNAG 3 cut(s) 24, 539, 903
DpnI GATC 7 cut(s) 354, 360, 493, 612, 830, 879, 1011
DpnII GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
DraIII CACNNNGTG 1 cut(s) 334
DrdI GACNNNNNNGTC 1 cut(s) 1271
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 1271
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 694, 1177
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 694, 1177
Ecl136II GAGCTC 1 cut(s) 1170
Eco24I GRGCYC 2 cut(s) 790, 1172
Eco32I GATATC 1 cut(s) 850
Eco53kI GAGCTC 1 cut(s) 1170
Eco88I CYCGRG 1 cut(s) 1248
EcoICRI GAGCTC 1 cut(s) 1170
EcoO109I RGGNCCY 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 3 cut(s) 158, 317, 418
EcoRV GATATC 1 cut(s) 850
EcoT38I GRGCYC 2 cut(s) 790, 1172
FaeI CATG 7 cut(s) 107, 176, 224, 480, 524, 798, 1309
FaqI GGGAC 3 cut(s) 13, 955, 1259
FatI CATG 7 cut(s) 103, 172, 220, 476, 520, 794, 1305
FauI CCCGC 2 cut(s) 38, 144
FauNDI CATATG 1 cut(s) 1036
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 2 cut(s) 82, 315
FokI GGATG 4 cut(s) 443, 621, 1002, 1097
FriOI GRGCYC 2 cut(s) 790, 1172
Fsp4HI GCNGC 2 cut(s) 82, 315
FspBI CTAG 2 cut(s) 750, 1191
GluI GCNGC 2 cut(s) 82, 315
GsaI CCCAGC 2 cut(s) 593, 976
HaeIII GGCC 2 cut(s) 110, 394
HapII CCGG 1 cut(s) 763
HgaI GACGC 2 cut(s) 851, 1023
Hin1II CATG 7 cut(s) 107, 176, 224, 480, 524, 798, 1309
HinfI GANTC 9 cut(s) 11, 26, 42, 87, 413, 437, 563, 597, 1076
HpaII CCGG 1 cut(s) 763
HphI GGTGA 3 cut(s) 148, 408, 1100
Hpy166II GTNNAC 6 cut(s) 217, 234, 337, 578, 934, 1091
Hpy188I TCNGA 5 cut(s) 25, 298, 491, 540, 1177
Hpy188III TCNNGA 8 cut(s) 8, 143, 194, 356, 382, 410, 428, 1324
Hpy8I GTNNAC 6 cut(s) 217, 234, 337, 578, 934, 1091
Hpy99I CGWCG 1 cut(s) 1017
HpyAV CCTTC 3 cut(s) 69, 193, 1114
HpyCH4III ACNGT 2 cut(s) 982, 1270
HpyCH4IV ACGT 1 cut(s) 962
HpyCH4V TGCA 7 cut(s) 84, 337, 350, 666, 1031, 1133, 1309
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 1014
HpyF3I CTNAG 3 cut(s) 24, 539, 903
HpySE526I ACGT 1 cut(s) 962
Hsp92II CATG 7 cut(s) 107, 176, 224, 480, 524, 798, 1309
KpnI GGTACC 1 cut(s) 166
Kzo9I GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
LguI GCTCTTC 1 cut(s) 1177
LmnI GCTCC 3 cut(s) 73, 407, 517
Lsp1109I GCAGC 2 cut(s) 68, 301
LweI GCATC 1 cut(s) 675
MabI ACCWGGT 1 cut(s) 158
MaeI CTAG 2 cut(s) 750, 1191
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 4 cut(s) 284, 604, 700, 758
MalI GATC 7 cut(s) 354, 360, 493, 612, 830, 879, 1011
MboI GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
MboII GAAGA 5 cut(s) 177, 558, 681, 952, 1164
MfeI CAATTG 2 cut(s) 34, 581
MflI RGATCY 3 cut(s) 352, 491, 610
MhlI GDGCHC 5 cut(s) 70, 339, 590, 790, 1172
MlsI TGGCCA 1 cut(s) 110
MluI ACGCGT 1 cut(s) 572
MluNI TGGCCA 1 cut(s) 110
MlyI GAGTC 2 cut(s) 35, 36
Mox20I TGGCCA 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 110
MseI TTAA 2 cut(s) 966, 1050
MslI CAYNNNNRTG 4 cut(s) 104, 583, 1304, 1310
Msp20I TGGCCA 1 cut(s) 110
MspI CCGG 1 cut(s) 763
MspR9I CCNGG 3 cut(s) 160, 319, 420
MunI CAATTG 2 cut(s) 34, 581
MvaI CCWGG 3 cut(s) 160, 319, 420
MvnI CGCG 3 cut(s) 574, 643, 1008
MwoI GCNNNNNNNGC 2 cut(s) 334, 1014
NdeI CATATG 1 cut(s) 1036
NdeII GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
NlaIII CATG 7 cut(s) 107, 176, 224, 480, 524, 798, 1309
NlaIV GGNNCC 2 cut(s) 164, 403
NmuCI GTSAC 1 cut(s) 604
NspI RCATGY 2 cut(s) 176, 1309
OliI CACNNNNGTG 1 cut(s) 583
PaeR7I CTCGAG 1 cut(s) 1248
PciSI GCTCTTC 1 cut(s) 1177
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 7 cut(s) 11, 87, 413, 437, 563, 597, 1076
PkrI GCNGC 2 cut(s) 83, 316
Ple19I CGATCG 1 cut(s) 1012
PleI GAGTC 2 cut(s) 34, 36
PpsI GAGTC 2 cut(s) 34, 36
Psp124BI GAGCTC 1 cut(s) 1172
Psp6I CCWGG 3 cut(s) 158, 317, 418
PspFI CCCAGC 2 cut(s) 589, 972
PspGI CCWGG 3 cut(s) 158, 317, 418
PspN4I GGNNCC 2 cut(s) 164, 403
PspPI GGNCC 1 cut(s) 392
PspXI VCTCGAGB 1 cut(s) 1248
PstI CTGCAG 1 cut(s) 86
PstNI CAGNNNCTG 2 cut(s) 356, 614
PsuI RGATCY 3 cut(s) 352, 491, 610
PvuI CGATCG 1 cut(s) 1012
RsaI GTAC 6 cut(s) 164, 510, 577, 649, 870, 1316
RsaNI GTAC 6 cut(s) 163, 509, 576, 648, 869, 1315
RseI CAYNNNNRTG 4 cut(s) 104, 583, 1304, 1310
SacI GAGCTC 1 cut(s) 1172
SapI GCTCTTC 1 cut(s) 1177
SaqAI TTAA 2 cut(s) 966, 1050
SatI GCNGC 2 cut(s) 82, 315
Sau3AI GATC 7 cut(s) 352, 358, 491, 610, 828, 877, 1009
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 2 cut(s) 35, 36
ScrFI CCNGG 3 cut(s) 160, 319, 420
SduI GDGCHC 5 cut(s) 70, 339, 590, 790, 1172
SexAI ACCWGGT 1 cut(s) 158
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 2 cut(s) 82, 1226
Sfr274I CTCGAG 1 cut(s) 1248
SlaI CTCGAG 1 cut(s) 1248
SmiMI CAYNNNNRTG 4 cut(s) 104, 583, 1304, 1310
SmlI CTYRAG 3 cut(s) 6, 1043, 1248
SmoI CTYRAG 3 cut(s) 6, 1043, 1248
SpeI ACTAGT 1 cut(s) 749
SsiI CCGC 4 cut(s) 31, 137, 641, 1006
SspI AATATT 2 cut(s) 503, 727
SspMI CTAG 2 cut(s) 750, 1191
SstI GAGCTC 1 cut(s) 1172
StyD4I CCNGG 3 cut(s) 158, 317, 418
TaaI ACNGT 2 cut(s) 982, 1270
TaiI ACGT 1 cut(s) 965
TaqI TCGA 4 cut(s) 268, 600, 1012, 1249
TaqII GACCGA 1 cut(s) 965
TatI WGTACW 2 cut(s) 647, 1314
TfiI GAWTC 7 cut(s) 11, 87, 413, 437, 563, 597, 1076
Tru1I TTAA 2 cut(s) 966, 1050
Tru9I TTAA 2 cut(s) 966, 1050
TscAI CASTG 2 cut(s) 248, 375
TseFI GTSAC 1 cut(s) 604
TseI GCWGC 2 cut(s) 81, 314
Tsp45I GTSAC 1 cut(s) 604
TspDTI ATGAA 4 cut(s) 92, 783, 1068, 1116
TspGWI ACGGA 2 cut(s) 530, 1100
TspRI CASTG 2 cut(s) 248, 375
VneI GTGCAC 1 cut(s) 335
XapI RAATTY 5 cut(s) 148, 548, 889, 1194, 1221
XceI RCATGY 2 cut(s) 176, 1309
XhoI CTCGAG 1 cut(s) 1248
XmiI GTMKAC 1 cut(s) 933
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 2 cut(s) 750, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.