Rh7DG464600

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001088
Physical Location & Seq
Forward (+)
66161760 .. 66169256
7497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG464600.1

Sequence Viewer

Length: 1971 bp
ATGGATCCTCCTCAAAATCCCAATGTGGGTTCTTCTTCTTCTCGAAGACCTGATGTGGGTTCTTCTTCTTCTTCAAGACCTGATGCGGGTTCTTCTTCTTCTTCAAGACCTGGTGTGGGTTCTTCTTCTTCTTCTCGAAGACCTGATGTGGGTTCTTCTTCTTCTTCGTCTCCAAGACTTGAAGTGGGTTCTTCTTCTTCATCTTCTTTGAGCTTCCCGAGATTCAGTCTCAACGTGAGGATGGGCACCACACAATCAATTACACCAGTCTTCGGCTGCAGAATCGACCCTACAGCTCGAGTGCCCAATAAGGACGATGAAGTTCCCCCCGAGCACGAGTTCAGTTTCAAATGGTATCGGGAGTATATTAGCCCTTCTATCCCCAGAGACAAGATCAAGTGCTCTGTCCACCCTGATGAGGTGGCCACTTTCCAGTGTAATGATTGTGTCAAATTGAAGTTACCATTCAAGGGAAGTTATCATTGCTCTCCCACTTGTTATTCTGAGGCATGGGAGAAACACAGGATGTGCCATCGTTGTGCTAAAGCTGTTGGTGAGACTTCTTCTGATAATCAACAACCAGAAGAAGAGATGTATGGCTGGCTTGACTTTGAAGATGATCCACTCAATGAAATTCCAAAGCTGGAACGAGATGGAAAACAATGGGTTGAGGTGTGCTCTTCAAGGTGTTATGTACCCACAGTGAATGACCTTAACTGCAGTTTGATGCTGGAATCTAAGGCTGTTGATTGTTCAACTGGTCTGGATTTGTGCCTGAGTGACATCATCATGACGGTTCCTGTTCTTTATCCGCCTCATCAGAAACCTCGCTCCATGCTTGAGTGTCCGCGGAAGCTTGGGGACTCTGATCTCAAAGCGCACACTTGCAGTGGTGTAAAATTTAGTGTGCTATCATATAATGTCCTTGGTAATCTTTATACTGATCCGTTTAGGTACTCTTACTGCCCAGAATGGGCTAGGTCTTGGGAATATCGTCAGCAGAATTTACTAAACGAGATCATTGAATATGATGCAGACATCCTCTGTCTTCAAGAGGTACAAAGTAATCATCTTGAGTATTTCTTCGAACCTGAGTTGGCAAAATGTGGATATTCTGTTATATACAAGAAGAGAAGAGGATCATTTTTTGCAAGAGACGGGCTTTGCATGGATGGTTGTGCTACATTTTACCGCCGAGATGTATTCAAAGAAATCGTAAAATATGAGTGTGCGTTTGATAGCTCTGTTATGAGAGTGATAGAAGCATTGCAGCCATTGGAACCTCAACAGAGAGATAGAGCTTGTATAAGGCTGAAGAAGGACAATGTTGCACTTGTTGTTATATTAGAAAAACTGGAAAGTGAGGGCACTTGCGATGGAATTCAACCTAGAATTTGTGTGGCAAATACGCACATATCTGCAAATCCAAAGTTACCAGATGTAAAATTGTTCCAGGTTGTGGACCTGATTGCTGGACTGGACAAAATTGCCAAGATGAAAATTCCTCTGTTGGTTTGTGGAGATTTCAACTCTCTTCCTCTGAGTGAGCCTCATAAGTTCATAACTGCTGGCAGAGTCGACAATCTTCCCAACGATACTGATCCATTTGGCATATGTCAGCATCTAAAGCTTCACCATTCACTTCCACTGAAAAGTGCATATGCATCTTTTTATAACTCGGATGGTATCGAGGAGGAACAACGTAGGAAGTTCAATTCTGAAAGCAGGGAGCCTTTGTTCACCCACTTCACACCAAGTTTCACAGGAACCCTAGATTACCTATTCTACACAGAGGATAGTTTGACGCTTGATGGTGTGTTGGAGCTACTTGACCGTGACAATCTGGGTGTTGGTATTCCATCTCCTTTTTGGTCATCAGATCATATTGCCCTCATGGCACGGTTTGTCCTCAATTCAACCTCTCCCCAGCAACAGGGTTCACAAGCAATTCCTGCTGACTTGTGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

656

Amino Acids

73.7

Weight (kDa)

5.51

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 304 - 628 6.2e-23 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1674
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 1459
AccI GTMKAC 1 cut(s) 1578
AccII CGCG 1 cut(s) 850
AciI CCGC 5 cut(s) 86, 812, 848, 850, 1192
AclWI GGATC 5 cut(s) 12, 614, 938, 1147, 1595
AcoI YGGCCR 2 cut(s) 423, 1964
AcsI RAATTY 6 cut(s) 633, 899, 1003, 1380, 1392, 1500
AcuI CTGAAG 1 cut(s) 1334
AfaI GTAC 3 cut(s) 696, 956, 1059
AjnI CCWGG 2 cut(s) 109, 1452
AloI GAACNNNNNNTCC 2 cut(s) 1721, 1753
AluBI AGCT 9 cut(s) 213, 296, 548, 643, 856, 1242, 1301, 1630, 1825
AluI AGCT 9 cut(s) 213, 296, 548, 643, 856, 1242, 1301, 1630, 1825
Alw21I GWGCWC 3 cut(s) 336, 404, 680
Alw26I GTCTC 5 cut(s) 174, 233, 381, 551, 1149
AlwI GGATC 5 cut(s) 12, 614, 938, 1147, 1595
Ama87I CYCGRG 3 cut(s) 217, 297, 329
AoxI GGCC 2 cut(s) 423, 1964
ApeKI GCWGC 2 cut(s) 276, 1270
ApoI RAATTY 6 cut(s) 633, 899, 1003, 1380, 1392, 1500
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 1 cut(s) 1462
AsuHPI GGTGA 3 cut(s) 566, 1625, 1732
AsuII TTCGAA 1 cut(s) 1086
AvaI CYCGRG 3 cut(s) 217, 297, 329
AvaII GGWCC 1 cut(s) 1462
BaeGI GKGCMC 3 cut(s) 248, 306, 1370
BalI TGGCCA 2 cut(s) 425, 1966
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 245
BauI CACGAG 1 cut(s) 335
BbsI GAAGAC 4 cut(s) 52, 145, 262, 1040
Bbv12I GWGCWC 3 cut(s) 336, 404, 680
BbvI GCAGC 2 cut(s) 263, 1282
BccI CCATC 8 cut(s) 235, 540, 647, 1166, 1370, 1676, 1805, 1867
BciT130I CCWGG 2 cut(s) 111, 1454
BcoDI GTCTC 5 cut(s) 174, 233, 381, 551, 1149
BfaI CTAG 3 cut(s) 978, 1389, 1772
BfmI CTRYAG 3 cut(s) 277, 291, 718
BglI GCCNNNNNGGC 1 cut(s) 1895
BisI GCNGC 2 cut(s) 277, 1271
BlsI GCNGC 2 cut(s) 278, 1272
Bme1390I CCNGG 2 cut(s) 111, 1454
Bme18I GGWCC 1 cut(s) 1462
BmeT110I CYCGRG 3 cut(s) 217, 297, 329
BmgT120I GGNCC 1 cut(s) 1462
BmiI GGNNCC 6 cut(s) 6, 247, 798, 1281, 1731, 1768
BmrFI CCNGG 2 cut(s) 111, 1454
BmsI GCATC 5 cut(s) 73, 717, 1021, 1630, 1673
BpiI GAAGAC 4 cut(s) 52, 145, 262, 1040
BplI GAGNNNNNCTC 4 cut(s) 662, 694, 1534, 1566
Bpu14I TTCGAA 1 cut(s) 1086
BpuEI CTTGAG 2 cut(s) 860, 1094
BsaBI GATNNNNATC 1 cut(s) 1599
BsaJI CCNNGG 2 cut(s) 848, 925
BsaXI ACNNNNNCTCC 2 cut(s) 1721, 1751
Bse1I ACTGG 5 cut(s) 266, 433, 763, 1359, 1482
Bse3DI GCAATG 2 cut(s) 481, 1265
Bse8I GATNNNNATC 1 cut(s) 1599
BseBI CCWGG 2 cut(s) 111, 1454
BseDI CCNNGG 2 cut(s) 848, 925
BseGI GGATG 5 cut(s) 246, 531, 1038, 1177, 1687
BseJI GATNNNNATC 1 cut(s) 1599
BseMI GCAATG 2 cut(s) 481, 1265
BseMII CTCAG 4 cut(s) 495, 767, 1083, 1532
BseNI ACTGG 5 cut(s) 266, 433, 763, 1359, 1482
BseRI GAGGAG 1 cut(s) 1706
BseSI GKGCMC 3 cut(s) 248, 306, 1370
BseXI GCAGC 2 cut(s) 263, 1282
BseYI CCCAGC 1 cut(s) 1926
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 2 cut(s) 425, 1966
BshNI GGYRCC 1 cut(s) 245
BsiHKAI GWGCWC 3 cut(s) 336, 404, 680
BsiHKCI CYCGRG 3 cut(s) 217, 297, 329
BslFI GGGAC 1 cut(s) 875
BsmAI GTCTC 5 cut(s) 174, 233, 381, 551, 1149
BsmBI CGTCTC 2 cut(s) 174, 1149
BsmFI GGGAC 1 cut(s) 875
BsnI GGCC 2 cut(s) 425, 1966
BsoBI CYCGRG 3 cut(s) 217, 297, 329
Bsp119I TTCGAA 1 cut(s) 1086
Bsp1286I GDGCHC 6 cut(s) 248, 306, 336, 404, 680, 1370
Bsp143I GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
BspACI CCGC 5 cut(s) 86, 812, 848, 850, 1192
BspANI GGCC 2 cut(s) 425, 1966
BspCNI CTCAG 4 cut(s) 496, 768, 1084, 1533
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 6 cut(s) 6, 247, 798, 1281, 1731, 1768
BspMAI CTGCAG 2 cut(s) 281, 722
BspPI GGATC 5 cut(s) 12, 614, 938, 1147, 1595
BspQI GCTCTTC 1 cut(s) 685
BspT104I TTCGAA 1 cut(s) 1086
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 481, 1265
BsrI ACTGG 5 cut(s) 266, 433, 763, 1359, 1482
BssECI CCNNGG 2 cut(s) 848, 925
BssMI GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 925
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 2 cut(s) 111, 1454
Bst4CI ACNGT 4 cut(s) 703, 796, 1835, 1902
Bst6I CTCTTC 5 cut(s) 582, 685, 1124, 1129, 1539
BstAPI GCANNNNNTGC 1 cut(s) 1952
BstBI TTCGAA 1 cut(s) 1086
BstC8I GCNNGC 2 cut(s) 602, 1570
BstDEI CTNAG 5 cut(s) 504, 738, 776, 1092, 1541
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 5 cut(s) 246, 531, 1038, 1177, 1687
BstFNI CGCG 1 cut(s) 850
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 9 cut(s) 7, 396, 622, 871, 946, 1020, 1142, 1603, 1882
BstMAI GTCTC 5 cut(s) 174, 233, 381, 551, 1149
BstMBI GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
BstMWI GCNNNNNNNGC 3 cut(s) 1627, 1895, 1952
BstNI CCWGG 2 cut(s) 111, 1454
BstSCI CCNGG 2 cut(s) 109, 1452
BstSFI CTRYAG 3 cut(s) 277, 291, 718
BstSLI GKGCMC 3 cut(s) 248, 306, 1370
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 2 cut(s) 263, 1282
BstV2I GAAGAC 4 cut(s) 52, 145, 262, 1040
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 425, 1966
BtgI CCRYGG 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 1389
BtsCI GGATG 5 cut(s) 246, 531, 1038, 1177, 1687
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 4 cut(s) 440, 708, 895, 1646
Cac8I GCNNGC 2 cut(s) 602, 1570
CciI TCATGA 1 cut(s) 789
CfoI GCGC 1 cut(s) 880
Cfr13I GGNCC 1 cut(s) 1462
Cfr42I CCGCGG 1 cut(s) 851
CseI GACGC 1 cut(s) 1813
CsiI ACCWGGT 1 cut(s) 109
Csp6I GTAC 3 cut(s) 695, 955, 1058
CviAII CATG 5 cut(s) 510, 790, 835, 1168, 1894
CviQI GTAC 3 cut(s) 695, 955, 1058
DdeI CTNAG 5 cut(s) 504, 738, 776, 1092, 1541
DpnI GATC 9 cut(s) 6, 395, 621, 870, 945, 1019, 1141, 1602, 1881
DpnII GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
EaeI YGGCCR 2 cut(s) 423, 1964
Eam1104I CTCTTC 5 cut(s) 582, 685, 1124, 1129, 1539
EarI CTCTTC 5 cut(s) 582, 685, 1124, 1129, 1539
EciI GGCGGA 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 925
Eco47I GGWCC 1 cut(s) 1462
Eco57I CTGAAG 1 cut(s) 1334
Eco88I CYCGRG 3 cut(s) 217, 297, 329
EcoRI GAATTC 1 cut(s) 1380
EcoRII CCWGG 2 cut(s) 109, 1452
EcoT14I CCWWGG 1 cut(s) 925
EcoT22I ATGCAT 1 cut(s) 1666
ErhI CCWWGG 1 cut(s) 925
Esp3I CGTCTC 2 cut(s) 174, 1149
FaeI CATG 5 cut(s) 513, 793, 838, 1171, 1897
FaqI GGGAC 1 cut(s) 875
FatI CATG 5 cut(s) 509, 789, 834, 1167, 1893
FauI CCCGC 1 cut(s) 79
FauNDI CATATG 2 cut(s) 1613, 1660
FblI GTMKAC 1 cut(s) 1578
Fnu4HI GCNGC 2 cut(s) 277, 1271
FokI GGATG 5 cut(s) 253, 538, 1025, 1184, 1694
Fsp4HI GCNGC 2 cut(s) 277, 1271
FspBI CTAG 3 cut(s) 978, 1389, 1772
GlaI GCGC 1 cut(s) 879
GluI GCNGC 2 cut(s) 277, 1271
GsaI CCCAGC 1 cut(s) 1930
HaeIII GGCC 2 cut(s) 425, 1966
HgaI GACGC 1 cut(s) 1813
HhaI GCGC 1 cut(s) 880
Hin1II CATG 5 cut(s) 513, 793, 838, 1171, 1897
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 1 cut(s) 1579
HindII GTYRAC 1 cut(s) 1579
HindIII AAGCTT 2 cut(s) 854, 1628
HinfI GANTC 5 cut(s) 222, 282, 734, 863, 1575
HphI GGTGA 3 cut(s) 566, 1625, 1732
Hpy166II GTNNAC 5 cut(s) 409, 1462, 1579, 1740, 1940
Hpy188I TCNGA 8 cut(s) 505, 568, 822, 868, 1542, 1681, 1720, 1879
Hpy8I GTNNAC 5 cut(s) 409, 1462, 1579, 1740, 1940
HpyAV CCTTC 2 cut(s) 384, 1312
HpyCH4III ACNGT 4 cut(s) 703, 796, 1835, 1902
HpyCH4IV ACGT 2 cut(s) 234, 1702
HpyF10VI GCNNNNNNNGC 3 cut(s) 1627, 1895, 1952
HpyF3I CTNAG 5 cut(s) 504, 738, 776, 1092, 1541
HpySE526I ACGT 2 cut(s) 234, 1702
Hsp92II CATG 5 cut(s) 513, 793, 838, 1171, 1897
HspAI GCGC 1 cut(s) 878
KspI CCGCGG 1 cut(s) 851
Kzo9I GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
LguI GCTCTTC 1 cut(s) 685
LmnI GCTCC 3 cut(s) 836, 1729, 1822
Lsp1109I GCAGC 2 cut(s) 263, 1282
LweI GCATC 5 cut(s) 73, 717, 1021, 1630, 1673
MabI ACCWGGT 1 cut(s) 109
MaeI CTAG 3 cut(s) 978, 1389, 1772
MaeII ACGT 2 cut(s) 234, 1702
MaeIII GTNAC 4 cut(s) 459, 779, 1431, 1835
MalI GATC 9 cut(s) 6, 395, 621, 870, 945, 1019, 1141, 1602, 1881
MboI GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 6 cut(s) 248, 306, 336, 404, 680, 1370
MlsI TGGCCA 2 cut(s) 425, 1966
MluNI TGGCCA 2 cut(s) 425, 1966
MlyI GAGTC 2 cut(s) 857, 1584
MmeI TCCRAC 1 cut(s) 1800
Mox20I TGGCCA 2 cut(s) 425, 1966
Mph1103I ATGCAT 1 cut(s) 1666
MscI TGGCCA 2 cut(s) 425, 1966
MseI TTAA 1 cut(s) 714
MslI CAYNNNNRTG 3 cut(s) 414, 537, 788
Msp20I TGGCCA 2 cut(s) 425, 1966
MspA1I CMGCKG 1 cut(s) 850
MspR9I CCNGG 2 cut(s) 111, 1454
MvaI CCWGG 2 cut(s) 111, 1454
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 3 cut(s) 1627, 1895, 1952
NdeI CATATG 2 cut(s) 1613, 1660
NdeII GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
NlaIII CATG 5 cut(s) 513, 793, 838, 1171, 1897
NlaIV GGNNCC 6 cut(s) 6, 247, 798, 1281, 1731, 1768
NmeAIII GCCGAG 1 cut(s) 1220
NmuCI GTSAC 2 cut(s) 779, 1835
NsiI ATGCAT 1 cut(s) 1666
NspV TTCGAA 1 cut(s) 1086
PaeR7I CTCGAG 1 cut(s) 297
PagI TCATGA 1 cut(s) 789
PciSI GCTCTTC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 222, 282, 734
PflMI CCANNNNNTGG 1 cut(s) 1459
PkrI GCNGC 2 cut(s) 278, 1272
PleI GAGTC 2 cut(s) 857, 1583
PpsI GAGTC 2 cut(s) 857, 1583
PsiI TTATAA 1 cut(s) 1674
Psp6I CCWGG 2 cut(s) 109, 1452
PspFI CCCAGC 1 cut(s) 1926
PspGI CCWGG 2 cut(s) 109, 1452
PspN4I GGNNCC 6 cut(s) 6, 247, 798, 1281, 1731, 1768
PspPI GGNCC 1 cut(s) 1462
PspXI VCTCGAGB 1 cut(s) 297
PstI CTGCAG 2 cut(s) 281, 722
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 3 cut(s) 696, 956, 1059
RsaNI GTAC 3 cut(s) 695, 955, 1058
RseI CAYNNNNRTG 3 cut(s) 414, 537, 788
SacII CCGCGG 1 cut(s) 851
SalI GTCGAC 1 cut(s) 1577
SapI GCTCTTC 1 cut(s) 685
SaqAI TTAA 1 cut(s) 714
SatI GCNGC 2 cut(s) 277, 1271
Sau3AI GATC 9 cut(s) 4, 393, 619, 868, 943, 1017, 1139, 1600, 1879
Sau96I GGNCC 1 cut(s) 1462
SchI GAGTC 2 cut(s) 857, 1584
ScrFI CCNGG 2 cut(s) 111, 1454
SduI GDGCHC 6 cut(s) 248, 306, 336, 404, 680, 1370
SexAI ACCWGGT 1 cut(s) 109
SfaNI GCATC 5 cut(s) 73, 717, 1021, 1630, 1673
SfcI CTRYAG 3 cut(s) 277, 291, 718
Sfr274I CTCGAG 1 cut(s) 297
Sfr303I CCGCGG 1 cut(s) 851
SfuI TTCGAA 1 cut(s) 1086
SgrBI CCGCGG 1 cut(s) 851
SinI GGWCC 1 cut(s) 1462
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 3 cut(s) 414, 537, 788
SmlI CTYRAG 3 cut(s) 297, 839, 1073
SmoI CTYRAG 3 cut(s) 297, 839, 1073
SsiI CCGC 5 cut(s) 86, 812, 848, 850, 1192
SspMI CTAG 3 cut(s) 978, 1389, 1772
StyD4I CCNGG 2 cut(s) 109, 1452
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 4 cut(s) 703, 796, 1835, 1902
TaiI ACGT 2 cut(s) 237, 1705
TaqI TCGA 7 cut(s) 43, 136, 285, 298, 1086, 1578, 1689
TfiI GAWTC 3 cut(s) 222, 282, 734
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TscAI CASTG 4 cut(s) 440, 708, 895, 1653
TseFI GTSAC 2 cut(s) 779, 1835
TseI GCWGC 2 cut(s) 276, 1270
Tsp45I GTSAC 2 cut(s) 779, 1835
TspDTI ATGAA 5 cut(s) 189, 333, 645, 1511, 1549
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 4 cut(s) 440, 708, 895, 1653
Van91I CCANNNNNTGG 1 cut(s) 1459
VpaK11BI GGWCC 1 cut(s) 1462
XapI RAATTY 6 cut(s) 633, 899, 1003, 1380, 1392, 1500
XcmI CCANNNNNNNNNTGG 1 cut(s) 1866
XhoI CTCGAG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 1578
XspI CTAG 3 cut(s) 978, 1389, 1772
Zsp2I ATGCAT 1 cut(s) 1666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.