Rmu_sc0000868.1_g000004

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000868.1
OG0001088
Physical Location & Seq
Forward (+)
20310 .. 26469
6160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000868.1_g000004.1.cds

Sequence Viewer

Length: 1968 bp
atggatcctgcacgcaatgctggttctcatgcccggaatcttggaacggattccctttctcgccaaaaagctacgaggaaagaacgggcctcttcttgttcttcttcatcgacatgccaattcagactccaaggaatgatgggtacaaaacgcagggtcactccagtttttggctgcaaaatcgacctagaagctcgcgtatacaggaagaacaccaccacattccatgaagaaccccccaagcatgagatcacgtacagatggtatcgggaatatgttagctcttcttcccccacagacaagatcaagtgttctgtccatccggataaagtagccactgtgcagtgcagaggctgtgtgaaactgaagttatctcttaccggaagctatcattgttcttctgaatgttactataattcccccgaaagacacaggatgtgccatcgttgcgctaaaacttttggtgagacatctattgaggaagaagagatgctctcagggttgactgcagctcaagattcattggaaaatcacaaactagaaagagatggaaaacagtgggttgaggtgtgctcttcaaagtcttactctcccacagcgcatgacattggctgcagtttacttctacagttcaaagctgtagatatttcagatggtattgatctgtgcccatgtgactttatcacaaccgataaagtgttgtatccgcctggccagaaacctcgctctatactcagttactgttggaagtctgcagcctttgatatcagagaaccatgttcagagggtgcaaggattagtatactgacctacaacatacttgccagttgttatgtcaaccagaagaagtacccggcctgcgcagaatgggctcgttcttgggaatatcgaagacacaatttaattcatgaaattactgggtatgatgcagacatcctctgtcttcaagaggtacaaagtaatcatcttgagaatttcttcaggcctgagttggaaaaatgcggatactctgttctatataagagaagaagagcaagttacctcgaaagagatagcaattcaatggacggatgtgccatattttaccgcaaaggtgtattcaaggagatcatgacatatgagcgtgcgtttgatagctctatattgagagcgattgaagaattgcagccattggaaactgaaggaccagatgaagctttccagaggctgaagaagtctgagtctgatagctctttaatgagagttatggaagcattggaacccttggaaccgaaacagagaaatgaagcttttgaaaggctgaagaaggataatgttgcactcatcgttatattagaaaaactggaaagtgagggcacttgtgatggcattcaacctagaatttgtgtggcaaatacgcacatatcggcaaatcccaacttcccagatgcaaaattgtttcaggttgtggacctgattactggactggacaaaattgccaagatgaaaattcctctgttggtttgtggagatttcaattctcttcctctgagtgagccttataggttcataactgctggccgacttgagaatcttcccaagaaggacaagaatagtgatccatttggcatatgtcagcatctaaaccttcaccattcaactcaactgaaaagtgcatatgcatcattccacaactcagatggtatcgaggaggaacaacgtaggaagttcaattctgaaagccaggagcctttgtttactcacttcacatcaagtttcacaggaaccctagattacatattctacacagaggatagtttgaagcttgatggtgtgttggagctacttgaccgtgacaatctgggagttggtattccatctcttctttggtcatcagatcatattgccctcatggcacggtttgcactcaattcaacctctccccagcaacagggctcacaagcaattcctgctaaactgtggccgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

655

Amino Acids

74.43

Weight (kDa)

6.69

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 862
AccB7I CCANNNNNTGG 1 cut(s) 170
AccI GTMKAC 2 cut(s) 201, 802
AccII CGCG 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 3 cut(s) 707, 1002, 1087
AclWI GGATC 2 cut(s) 12, 1592
AcoI YGGCCR 3 cut(s) 712, 1558, 1961
AcsI RAATTY 3 cut(s) 973, 1380, 1488
AcuI CTGAAG 5 cut(s) 386, 964, 1200, 1229, 1322
AfaI GTAC 4 cut(s) 145, 257, 851, 954
AfiI CCNNNNNNNGG 2 cut(s) 170, 1930
AjnI CCWGG 2 cut(s) 709, 1722
AloI GAACNNNNNNTCC 2 cut(s) 996, 1028
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 2 cut(s) 12, 1592
AlwNI CAGNNNCTG 3 cut(s) 354, 741, 1207
Aor13HI TCCGGA 1 cut(s) 322
AoxI GGCC 6 cut(s) 87, 712, 855, 983, 1558, 1961
ApeKI GCWGC 5 cut(s) 174, 509, 612, 755, 1165
ApoI RAATTY 3 cut(s) 973, 1380, 1488
Asp700I GAANNNNTTC 2 cut(s) 49, 977
AspLEI GCGC 3 cut(s) 452, 601, 863
AspS9I GGNCC 3 cut(s) 87, 1184, 1450
AsuC2I CCSGG 2 cut(s) 34, 854
AsuHPI GGTGA 2 cut(s) 476, 1622
AvaII GGWCC 2 cut(s) 1184, 1450
BaeGI GKGCMC 2 cut(s) 671, 1358
BalI TGGCCA 1 cut(s) 714
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 874, 1937
BbsI GAAGAC 2 cut(s) 898, 935
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 5 cut(s) 161, 521, 599, 767, 1177
BceAI ACGGC 1 cut(s) 1948
BciT130I CCWGG 2 cut(s) 711, 1724
BciVI GTATCC 2 cut(s) 714, 998
BcnI CCSGG 2 cut(s) 34, 854
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 4 cut(s) 188, 539, 1377, 1769
BfmI CTRYAG 5 cut(s) 507, 613, 626, 639, 753
BfuI GTATCC 2 cut(s) 714, 998
BglI GCCNNNNNGGC 1 cut(s) 1892
BisI GCNGC 5 cut(s) 175, 510, 613, 756, 1166
BlsI GCNGC 5 cut(s) 176, 511, 614, 757, 1167
Bme1390I CCNGG 4 cut(s) 34, 711, 854, 1724
Bme18I GGWCC 2 cut(s) 1184, 1450
BmgT120I GGNCC 3 cut(s) 87, 1184, 1450
BmiI GGNNCC 5 cut(s) 6, 1260, 1269, 1728, 1765
BmrFI CCNGG 4 cut(s) 34, 711, 854, 1724
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 5 cut(s) 480, 916, 1417, 1627, 1670
BmuI ACTGGG 1 cut(s) 927
BpiI GAAGAC 2 cut(s) 898, 935
BplI GAGNNNNNCTC 4 cut(s) 479, 511, 557, 589
BpmI CTGGAG 1 cut(s) 147
BpuEI CTTGAG 3 cut(s) 498, 989, 1586
BpuMI CCSGG 2 cut(s) 34, 854
BsaAI YACGTR 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 130, 1263
BsaWI WCCGGW 2 cut(s) 322, 380
BsaXI ACNNNNNCTCC 2 cut(s) 1718, 1748
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 1930
Bse1I ACTGG 6 cut(s) 164, 825, 922, 1347, 1465, 1470
Bse3DI GCAATG 1 cut(s) 22
BseAI TCCGGA 1 cut(s) 322
BseBI CCWGG 2 cut(s) 711, 1724
BseDI CCNNGG 2 cut(s) 130, 1263
BseGI GGATG 4 cut(s) 319, 441, 933, 1076
BseLI CCNNNNNNNGG 2 cut(s) 170, 1930
BseMI GCAATG 1 cut(s) 22
BseMII CTCAG 6 cut(s) 510, 748, 978, 1209, 1520, 1689
BseNI ACTGG 6 cut(s) 164, 825, 922, 1347, 1465, 1470
BseRI GAGGAG 1 cut(s) 1703
BseSI GKGCMC 2 cut(s) 671, 1358
BseXI GCAGC 5 cut(s) 161, 521, 599, 767, 1177
BseYI CCCAGC 1 cut(s) 1923
BsgI GTGCAG 2 cut(s) 362, 367
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 6 cut(s) 89, 714, 857, 985, 1560, 1963
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 4 cut(s) 34, 323, 381, 854
BslI CCNNNNNNNGG 2 cut(s) 170, 1930
BsmAI GTCTC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 1368
BsnI GGCC 6 cut(s) 89, 714, 857, 985, 1560, 1963
Bsp1286I GDGCHC 5 cut(s) 575, 671, 874, 1358, 1937
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
BspACI CCGC 3 cut(s) 707, 1002, 1087
BspANI GGCC 6 cut(s) 89, 714, 857, 985, 1560, 1963
BspCNI CTCAG 6 cut(s) 509, 747, 979, 1210, 1521, 1688
BspEI TCCGGA 1 cut(s) 322
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 2 cut(s) 907, 1110
BspLI GGNNCC 5 cut(s) 6, 1260, 1269, 1728, 1765
BspMAI CTGCAG 3 cut(s) 511, 617, 757
BspPI GGATC 2 cut(s) 12, 1592
BspQI GCTCTTC 3 cut(s) 289, 580, 1024
BsrDI GCAATG 1 cut(s) 22
BsrI ACTGG 6 cut(s) 164, 825, 922, 1347, 1465, 1470
BssECI CCNNGG 2 cut(s) 130, 1263
BssMI GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
BssNAI GTATAC 2 cut(s) 202, 803
BssT1I CCWWGG 2 cut(s) 130, 1263
Bst1107I GTATAC 2 cut(s) 202, 803
Bst2UI CCWGG 2 cut(s) 711, 1724
Bst4CI ACNGT 7 cut(s) 340, 558, 630, 743, 1832, 1899, 1959
Bst6I CTCTTC 7 cut(s) 97, 289, 480, 580, 1024, 1527, 1866
BstAPI GCANNNNNTGC 3 cut(s) 17, 1901, 1949
BstBAI YACGTR 1 cut(s) 255
BstC8I GCNNGC 5 cut(s) 13, 196, 859, 1125, 1558
BstDEI CTNAG 6 cut(s) 496, 734, 987, 1218, 1529, 1675
BstF5I GGATG 4 cut(s) 319, 441, 933, 1076
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 3 cut(s) 452, 601, 863
BstKTI GATC 7 cut(s) 7, 252, 306, 664, 1110, 1600, 1879
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
BstMWI GCNNNNNNNGC 6 cut(s) 17, 447, 869, 1892, 1901, 1949
BstNI CCWGG 2 cut(s) 711, 1724
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 4 cut(s) 32, 709, 852, 1722
BstSFI CTRYAG 5 cut(s) 507, 613, 626, 639, 753
BstSLI GKGCMC 2 cut(s) 671, 1358
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 5 cut(s) 161, 521, 599, 767, 1177
BstV2I GAAGAC 2 cut(s) 898, 935
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 2 cut(s) 202, 803
BsuI GTATCC 2 cut(s) 714, 998
BsuRI GGCC 6 cut(s) 89, 714, 857, 985, 1560, 1963
BtsCI GGATG 4 cut(s) 319, 441, 933, 1076
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 3 cut(s) 336, 350, 563
Cac8I GCNNGC 5 cut(s) 13, 196, 859, 1125, 1558
CaiI CAGNNNCTG 3 cut(s) 354, 741, 1207
CciI TCATGA 2 cut(s) 907, 1110
CfoI GCGC 3 cut(s) 452, 601, 863
Cfr13I GGNCC 3 cut(s) 87, 1184, 1450
Csp6I GTAC 4 cut(s) 144, 256, 850, 953
CviQI GTAC 4 cut(s) 144, 256, 850, 953
DdeI CTNAG 6 cut(s) 496, 734, 987, 1218, 1529, 1675
DpnI GATC 7 cut(s) 6, 251, 305, 663, 1109, 1599, 1878
DpnII GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
EaeI YGGCCR 3 cut(s) 712, 1558, 1961
Eam1104I CTCTTC 7 cut(s) 97, 289, 480, 580, 1024, 1527, 1866
EarI CTCTTC 7 cut(s) 97, 289, 480, 580, 1024, 1527, 1866
EciI GGCGGA 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 130, 1263
Eco147I AGGCCT 1 cut(s) 985
Eco24I GRGCYC 2 cut(s) 874, 1937
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 1184, 1450
Eco57I CTGAAG 5 cut(s) 386, 964, 1200, 1229, 1322
EcoRII CCWGG 2 cut(s) 709, 1722
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 2 cut(s) 130, 1263
EcoT22I ATGCAT 1 cut(s) 1663
EcoT38I GRGCYC 2 cut(s) 874, 1937
ErhI CCWWGG 2 cut(s) 130, 1263
FauNDI CATATG 3 cut(s) 1117, 1610, 1657
FblI GTMKAC 2 cut(s) 201, 802
Fnu4HI GCNGC 5 cut(s) 175, 510, 613, 756, 1166
FokI GGATG 4 cut(s) 306, 448, 920, 1083
FriOI GRGCYC 2 cut(s) 874, 1937
Fsp4HI GCNGC 5 cut(s) 175, 510, 613, 756, 1166
FspBI CTAG 4 cut(s) 188, 539, 1377, 1769
FspI TGCGCA 1 cut(s) 862
GlaI GCGC 3 cut(s) 451, 600, 862
GluI GCNGC 5 cut(s) 175, 510, 613, 756, 1166
GsaI CCCAGC 1 cut(s) 1927
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 6 cut(s) 89, 714, 857, 985, 1560, 1963
HapII CCGG 4 cut(s) 34, 323, 381, 854
HhaI GCGC 3 cut(s) 452, 601, 863
Hin6I GCGC 3 cut(s) 450, 599, 861
HinP1I GCGC 3 cut(s) 450, 599, 861
HincII GTYRAC 2 cut(s) 504, 838
HindII GTYRAC 2 cut(s) 504, 838
HindIII AAGCTT 3 cut(s) 1194, 1287, 1802
HinfI GANTC 6 cut(s) 37, 50, 126, 518, 1220, 1570
HpaII CCGG 4 cut(s) 34, 323, 381, 854
HphI GGTGA 2 cut(s) 476, 1622
Hpy166II GTNNAC 7 cut(s) 202, 504, 620, 803, 838, 1450, 1737
Hpy188III TCNNGA 8 cut(s) 269, 323, 515, 908, 947, 968, 1111, 1201
Hpy8I GTNNAC 7 cut(s) 202, 504, 620, 803, 838, 1450, 1737
HpyAV CCTTC 4 cut(s) 1175, 1300, 1576, 1637
HpyCH4III ACNGT 7 cut(s) 340, 558, 630, 743, 1832, 1899, 1959
HpyCH4IV ACGT 2 cut(s) 254, 1699
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 447, 869, 1892, 1901, 1949
HpyF3I CTNAG 6 cut(s) 496, 734, 987, 1218, 1529, 1675
HpySE526I ACGT 2 cut(s) 254, 1699
HspAI GCGC 3 cut(s) 450, 599, 861
Kpn2I TCCGGA 1 cut(s) 322
Kzo9I GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
LguI GCTCTTC 3 cut(s) 289, 580, 1024
LmnI GCTCC 2 cut(s) 1726, 1819
Lsp1109I GCAGC 5 cut(s) 161, 521, 599, 767, 1177
LweI GCATC 5 cut(s) 480, 916, 1417, 1627, 1670
MaeI CTAG 4 cut(s) 188, 539, 1377, 1769
MaeII ACGT 2 cut(s) 254, 1699
MaeIII GTNAC 6 cut(s) 157, 407, 674, 737, 1037, 1832
MalI GATC 7 cut(s) 6, 251, 305, 663, 1109, 1599, 1878
MboI GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 5 cut(s) 575, 671, 874, 1358, 1937
MlsI TGGCCA 1 cut(s) 714
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 2 cut(s) 120, 1229
MmeI TCCRAC 3 cut(s) 725, 972, 1797
Mox20I TGGCCA 1 cut(s) 714
Mph1103I ATGCAT 1 cut(s) 1663
MroI TCCGGA 1 cut(s) 322
MroXI GAANNNNTTC 2 cut(s) 49, 977
MscI TGGCCA 1 cut(s) 714
MseI TTAA 2 cut(s) 902, 1235
MslI CAYNNNNRTG 1 cut(s) 112
Msp20I TGGCCA 1 cut(s) 714
MspI CCGG 4 cut(s) 34, 323, 381, 854
MspR9I CCNGG 4 cut(s) 34, 711, 854, 1724
Mva1269I GAATGC 1 cut(s) 1368
MvaI CCWGG 2 cut(s) 711, 1724
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 6 cut(s) 17, 447, 869, 1892, 1901, 1949
NciI CCSGG 2 cut(s) 34, 854
NdeI CATATG 3 cut(s) 1117, 1610, 1657
NdeII GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
NlaIV GGNNCC 5 cut(s) 6, 1260, 1269, 1728, 1765
NmuCI GTSAC 3 cut(s) 157, 674, 1832
NsbI TGCGCA 1 cut(s) 862
NsiI ATGCAT 1 cut(s) 1663
NspI RCATGY 1 cut(s) 117
PagI TCATGA 2 cut(s) 907, 1110
PceI AGGCCT 1 cut(s) 985
PciSI GCTCTTC 3 cut(s) 289, 580, 1024
PctI GAATGC 1 cut(s) 1368
PdmI GAANNNNTTC 2 cut(s) 49, 977
PfeI GAWTC 4 cut(s) 37, 50, 518, 1570
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 5 cut(s) 176, 511, 614, 757, 1167
PleI GAGTC 2 cut(s) 120, 1228
PpsI GAGTC 2 cut(s) 120, 1228
Ppu21I YACGTR 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 709, 1722
PspFI CCCAGC 1 cut(s) 1923
PspGI CCWGG 2 cut(s) 709, 1722
PspN4I GGNNCC 5 cut(s) 6, 1260, 1269, 1728, 1765
PspPI GGNCC 3 cut(s) 87, 1184, 1450
PstI CTGCAG 3 cut(s) 511, 617, 757
PstNI CAGNNNCTG 3 cut(s) 354, 741, 1207
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 4 cut(s) 145, 257, 851, 954
RsaNI GTAC 4 cut(s) 144, 256, 850, 953
RseI CAYNNNNRTG 1 cut(s) 112
SapI GCTCTTC 3 cut(s) 289, 580, 1024
SaqAI TTAA 2 cut(s) 902, 1235
SatI GCNGC 5 cut(s) 175, 510, 613, 756, 1166
Sau3AI GATC 7 cut(s) 4, 249, 303, 661, 1107, 1597, 1876
Sau96I GGNCC 3 cut(s) 87, 1184, 1450
SchI GAGTC 2 cut(s) 120, 1229
ScrFI CCNGG 4 cut(s) 34, 711, 854, 1724
SduI GDGCHC 5 cut(s) 575, 671, 874, 1358, 1937
SfaNI GCATC 5 cut(s) 480, 916, 1417, 1627, 1670
SfcI CTRYAG 5 cut(s) 507, 613, 626, 639, 753
SinI GGWCC 2 cut(s) 1184, 1450
SmiMI CAYNNNNRTG 1 cut(s) 112
SmlI CTYRAG 3 cut(s) 513, 968, 1565
SmoI CTYRAG 3 cut(s) 513, 968, 1565
SseBI AGGCCT 1 cut(s) 985
SsiI CCGC 3 cut(s) 707, 1002, 1087
SspMI CTAG 4 cut(s) 188, 539, 1377, 1769
StuI AGGCCT 1 cut(s) 985
StyD4I CCNGG 4 cut(s) 32, 709, 852, 1722
StyI CCWWGG 2 cut(s) 130, 1263
TaaI ACNGT 7 cut(s) 340, 558, 630, 743, 1832, 1899, 1959
TaiI ACGT 2 cut(s) 257, 1702
TaqI TCGA 5 cut(s) 110, 183, 889, 1044, 1686
TfiI GAWTC 4 cut(s) 37, 50, 518, 1570
Tru1I TTAA 2 cut(s) 902, 1235
Tru9I TTAA 2 cut(s) 902, 1235
TscAI CASTG 3 cut(s) 343, 350, 563
TseFI GTSAC 3 cut(s) 157, 674, 1832
TseI GCWGC 5 cut(s) 174, 509, 612, 755, 1165
Tsp45I GTSAC 3 cut(s) 157, 674, 1832
TspDTI ATGAA 9 cut(s) 96, 243, 510, 896, 924, 1206, 1299, 1499, 1537
TspGWI ACGGA 2 cut(s) 62, 1083
TspRI CASTG 3 cut(s) 343, 350, 563
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 1184, 1450
XapI RAATTY 3 cut(s) 973, 1380, 1488
XceI RCATGY 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 2 cut(s) 1676, 1863
XmiI GTMKAC 2 cut(s) 201, 802
XmnI GAANNNNTTC 2 cut(s) 49, 977
XspI CTAG 4 cut(s) 188, 539, 1377, 1769
Zsp2I ATGCAT 1 cut(s) 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.