MD15G1401700.v1.1

threonine-tRNA ligase

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
Physical Location & Seq
Forward (+)
50249964 .. 50251479
1516 bp
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UTR
Exon/CDS
Intron
MD15G1401700.v1.1.491

Sequence Viewer

Length: 189 bp
ATGGGTGAAAGGCAACATCTGCCCTTACTCCTTAGAGATGCTTTGGATGATAAAGGTTGGAGCTATCAAATTGATGAAGGTGGTGGTGCCTTTTATGGTCCAAAGATTGATCTTAAGATTGAGGATGCCCTTGGAAGGAGGTGGCAGTGCTCAACTATACAAGTTAATCCTGAACTCATGACGGGATAA

Protein Analysis

63

Amino Acids

7.0

Weight (kDa)

4.83

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 135
AflII CTTAAG 1 cut(s) 113
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
Alw21I GWGCWC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 152
BfrI CTTAAG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 88
BmsI GCATC 2 cut(s) 28, 115
BsaJI CCNNGG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 52, 130
BseLI CCNNNNNNNGG 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 1 cut(s) 109
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 130
BstAFI CTTAAG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 52, 130
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 19
BtsCI GGATG 2 cut(s) 52, 130
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 152
CciI TCATGA 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 98
CviAII CATG 1 cut(s) 178
CviJI RGCY 1 cut(s) 63
CviKI_1 RGCY 1 cut(s) 63
DdeI CTNAG 1 cut(s) 32
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 181
FaiI YATR 3 cut(s) 96, 158, 179
FatI CATG 1 cut(s) 177
FokI GGATG 2 cut(s) 59, 137
Hin1II CATG 1 cut(s) 181
HphI GGTGA 1 cut(s) 17
Hpy188III TCNNGA 2 cut(s) 170, 178
HpyAV CCTTC 2 cut(s) 71, 129
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 1 cut(s) 32
Hsp92II CATG 1 cut(s) 181
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 1 cut(s) 183
LweI GCATC 2 cut(s) 28, 115
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 2 cut(s) 115, 132
MseI TTAA 2 cut(s) 114, 165
MspCI CTTAAG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeII GATC 1 cut(s) 109
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 88
PagI TCATGA 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 114, 165
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 152
SetI ASST 4 cut(s) 58, 65, 82, 143
SfaNI GCATC 2 cut(s) 28, 115
SgeI CNNG 3 cut(s) 143, 173, 182
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 1 cut(s) 69
StyI CCWWGG 1 cut(s) 130
TasI AATT 1 cut(s) 69
Tru1I TTAA 2 cut(s) 114, 165
Tru9I TTAA 2 cut(s) 114, 165
TscAI CASTG 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 90
TspRI CASTG 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.