Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004989

Physical Location & Seq

Genomic Coordinates Forward (+)

50284927 .. 50285992

Gene Structure 1066 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1402000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 534 bp

ATGGTCTTAAGCAGGCTCCTCGGGCCTAGTTTCACCGCTTTAGCTCAGTCATCAAGAGATGAAGCTCCCAAAAGAATAACCGCTCTCAGTGAACCATACAAATTGGAAATTTTGGATAGTATTAAAGAAGATCCCATTACCATATATCATATTGGTGATGAATGGTGGAACCTATGTGCGGGGCCCCATGTTGAAAAAATTGGGAATATTAACAGAAAACTTGTCGAACTTGAGTCTATGTTGGCGCCTTGGTCAAGTCAAATCAAAGATGAAATTAAGGGTGTCATAGATCTTACAGAAGAACTTTCGTTGCAATTCGGCTTCAGCAAATATGAGGTCAATCTCTTTACAAGGCTAGAGAAAGCTGTTGGAGATGATATATGGGTGAAAGCAACATATGCCCTTAAAGATGCTTTGGATGATAAAGGCTGGAGCTATCAAATTGATGAAGGCGATGGTGCCTTTTATGGTCCAAAAATTGATCTTAAGATTAAGGATGCCCTTGGAAGGAAGTGGCAGTGCTCAACTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_2b tRNA_SAD

Protein Analysis

178

Amino Acids

20.05

Weight (kDa)

4.94

Isoelectric Point (pI)

35.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_SAD	PF07973	46 - 71	1.2e-06	Threonyl and Alanyl tRNA synthetase second additional domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004989)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17960 AT1G17960
malus_domestica	MD15G1401700.v1.1 MD15G1402000.v1.1
rosa_chinensis	RchiOBHm_Chr4g0444231
rosa_laevigata	RLG00000005339 RLG00000005847 RLG00000008520 RLG00000020302
rosa_multiflora	Rmu_sc0000016.1_g000006 Rmu_sc0000987.1_g000022 Rmu_sc0014698.1_g000003
rosa_roxburghii	Rroxscaffold_3G00219500 Rroxscaffold_5G00384800
rosa_rugosa	Rorug03G0334500 Rorug07G0196800
rosa_samantha	Rh1BG152700 Rh1CG027000 Rh1DG185100 Rh2BG464200 Rh4AG158400 Rh4BG163900 Rh4CG184100 Rh4DG152900 Rh4DG153000 Rh5CG279500 Rh6BG190300 Rh7CG027100 Rh7DG026600 Rh7DG335900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	244, 458
AccBSI	CCGCTC	1 cut(s)	83
AciI	CCGC	3 cut(s)	36, 81, 179
AclWI	GGATC	1 cut(s)	125
AcsI	RAATTY	1 cut(s)	108
AcuI	CTGAAG	1 cut(s)	307
AcyI	GRCGYC	1 cut(s)	245
AfiI	CCNNNNNNNGG	2 cut(s)	178, 507
AflII	CTTAAG	2 cut(s)	7, 485
AgsI	TTSAA	1 cut(s)	194
AluBI	AGCT	4 cut(s)	44, 65, 365, 435
AluI	AGCT	4 cut(s)	44, 65, 365, 435
Alw21I	GWGCWC	1 cut(s)	524
AlwI	GGATC	1 cut(s)	125
Ama87I	CYCGRG	1 cut(s)	20
AoxI	GGCC	2 cut(s)	23, 182
ApaI	GGGCCC	1 cut(s)	186
ApoI	RAATTY	1 cut(s)	108
ArsI	GACNNNNNNTTYG	2 cut(s)	321, 353
AspLEI	GCGC	1 cut(s)	247
AspS9I	GGNCC	4 cut(s)	23, 182, 183, 470
AsuHPI	GGTGA	3 cut(s)	25, 167, 397
AvaI	CYCGRG	1 cut(s)	20
AvaII	GGWCC	1 cut(s)	470
BaeGI	GKGCMC	1 cut(s)	186
BanI	GGYRCC	2 cut(s)	244, 458
BanII	GRGCYC	1 cut(s)	186
Bbv12I	GWGCWC	1 cut(s)	524
BccI	CCATC	1 cut(s)	449
BcgI	CGANNNNNNTGC	1 cut(s)	35
BfaI	CTAG	2 cut(s)	27, 356
BfoI	RGCGCY	1 cut(s)	248
BfrI	CTTAAG	2 cut(s)	7, 485
BglII	AGATCT	1 cut(s)	289
Bme18I	GGWCC	1 cut(s)	470
BmeT110I	CYCGRG	1 cut(s)	20
BmgT120I	GGNCC	4 cut(s)	23, 182, 183, 470
BmiI	GGNNCC	7 cut(s)	17, 170, 183, 184, 185, 246, 460
BmsI	GCATC	2 cut(s)	400, 487
BpmI	CTGGAG	1 cut(s)	451
BpuEI	CTTGAG	1 cut(s)	251
BsaHI	GRCGYC	1 cut(s)	245
BsaJI	CCNNGG	3 cut(s)	19, 248, 502
Bsc4I	CCNNNNNNNGG	2 cut(s)	178, 507
BseDI	CCNNGG	3 cut(s)	19, 248, 502
BseGI	GGATG	2 cut(s)	424, 502
BseLI	CCNNNNNNNGG	2 cut(s)	178, 507
BseMII	CTCAG	2 cut(s)	59, 100
BseRI	GAGGAG	1 cut(s)	8
BseSI	GKGCMC	1 cut(s)	186
BshFI	GGCC	2 cut(s)	25, 184
BshNI	GGYRCC	2 cut(s)	244, 458
BsiHKAI	GWGCWC	1 cut(s)	524
BsiHKCI	CYCGRG	1 cut(s)	20
BslI	CCNNNNNNNGG	2 cut(s)	178, 507
BsnI	GGCC	2 cut(s)	25, 184
BsoBI	CYCGRG	1 cut(s)	20
Bsp120I	GGGCCC	1 cut(s)	182
Bsp1286I	GDGCHC	2 cut(s)	186, 524
Bsp143I	GATC	3 cut(s)	130, 289, 481
BspACI	CCGC	3 cut(s)	36, 81, 179
BspANI	GGCC	2 cut(s)	25, 184
BspCNI	CTCAG	2 cut(s)	58, 99
BspLI	GGNNCC	7 cut(s)	17, 170, 183, 184, 185, 246, 460
BspPI	GGATC	1 cut(s)	125
BspT107I	GGYRCC	2 cut(s)	244, 458
BspTI	CTTAAG	2 cut(s)	7, 485
BsrBI	CCGCTC	1 cut(s)	83
BssECI	CCNNGG	3 cut(s)	19, 248, 502
BssMI	GATC	3 cut(s)	130, 289, 481
BssNI	GRCGYC	1 cut(s)	245
BssT1I	CCWWGG	2 cut(s)	248, 502
BstACI	GRCGYC	1 cut(s)	245
BstAFI	CTTAAG	2 cut(s)	7, 485
BstAPI	GCANNNNNTGC	1 cut(s)	398
BstC8I	GCNNGC	1 cut(s)	14
BstDEI	CTNAG	2 cut(s)	45, 86
BstF5I	GGATG	2 cut(s)	424, 502
BstH2I	RGCGCY	1 cut(s)	248
BstHHI	GCGC	1 cut(s)	247
BstKTI	GATC	3 cut(s)	133, 292, 484
BstMBI	GATC	3 cut(s)	130, 289, 481
BstMWI	GCNNNNNNNGC	2 cut(s)	22, 398
BstSLI	GKGCMC	1 cut(s)	186
BstX2I	RGATCY	2 cut(s)	130, 289
BstYI	RGATCY	2 cut(s)	130, 289
BsuRI	GGCC	2 cut(s)	25, 184
BtgZI	GCGATG	1 cut(s)	468
BtsCI	GGATG	2 cut(s)	424, 502
BtsI	GCAGTG	1 cut(s)	524
BtsIMutI	CAGTG	2 cut(s)	94, 524
Cac8I	GCNNGC	1 cut(s)	14
CfoI	GCGC	1 cut(s)	247
Cfr13I	GGNCC	4 cut(s)	23, 182, 183, 470
CviAII	CATG	1 cut(s)	188
DdeI	CTNAG	2 cut(s)	45, 86
DinI	GGCGCC	1 cut(s)	246
DpnI	GATC	3 cut(s)	132, 291, 483
DpnII	GATC	3 cut(s)	130, 289, 481
Eco130I	CCWWGG	2 cut(s)	248, 502
Eco24I	GRGCYC	1 cut(s)	186
Eco47I	GGWCC	1 cut(s)	470
Eco57I	CTGAAG	1 cut(s)	307
Eco88I	CYCGRG	1 cut(s)	20
EcoO109I	RGGNCCY	2 cut(s)	182, 183
EcoT14I	CCWWGG	2 cut(s)	248, 502
EcoT38I	GRGCYC	1 cut(s)	186
EgeI	GGCGCC	1 cut(s)	246
EheI	GGCGCC	1 cut(s)	246
ErhI	CCWWGG	2 cut(s)	248, 502
FaeI	CATG	1 cut(s)	191
FatI	CATG	1 cut(s)	187
FauI	CCCGC	1 cut(s)	172
FauNDI	CATATG	1 cut(s)	397
FokI	GGATG	2 cut(s)	431, 509
FriOI	GRGCYC	1 cut(s)	186
FspBI	CTAG	2 cut(s)	27, 356
GlaI	GCGC	1 cut(s)	246
GsuI	CTGGAG	1 cut(s)	451
HaeII	RGCGCY	1 cut(s)	248
HaeIII	GGCC	2 cut(s)	25, 184
HhaI	GCGC	1 cut(s)	247
Hin1I	GRCGYC	1 cut(s)	245
Hin1II	CATG	1 cut(s)	191
Hin6I	GCGC	1 cut(s)	245
HinP1I	GCGC	1 cut(s)	245
HinfI	GANTC	1 cut(s)	233
HphI	GGTGA	3 cut(s)	25, 167, 397
Hpy166II	GTNNAC	1 cut(s)	92
Hpy188III	TCNNGA	1 cut(s)	54
Hpy8I	GTNNAC	1 cut(s)	92
HpyAV	CCTTC	2 cut(s)	443, 501
HpyCH4V	TGCA	1 cut(s)	313
HpyF10VI	GCNNNNNNNGC	2 cut(s)	22, 398
HpyF3I	CTNAG	2 cut(s)	45, 86
Hsp92I	GRCGYC	1 cut(s)	245
Hsp92II	CATG	1 cut(s)	191
HspAI	GCGC	1 cut(s)	245
KasI	GGCGCC	1 cut(s)	244
Kzo9I	GATC	3 cut(s)	130, 289, 481
LmnI	GCTCC	3 cut(s)	21, 70, 432
LpnPI	CCDG	1 cut(s)	415
LweI	GCATC	2 cut(s)	400, 487
MaeI	CTAG	2 cut(s)	27, 356
MalI	GATC	3 cut(s)	132, 291, 483
MbiI	CCGCTC	1 cut(s)	83
MboI	GATC	3 cut(s)	130, 289, 481
MboII	GAAGA	2 cut(s)	140, 311
MflI	RGATCY	2 cut(s)	130, 289
MhlI	GDGCHC	2 cut(s)	186, 524
MluCI	AATT	7 cut(s)	101, 108, 198, 273, 314, 441, 477
Mly113I	GGCGCC	1 cut(s)	245
MlyI	GAGTC	1 cut(s)	242
MmeI	TCCRAC	1 cut(s)	349
MnlI	CCTC	2 cut(s)	29, 328
MseI	TTAA	7 cut(s)	8, 123, 210, 276, 405, 486, 492
MslI	CAYNNNNRTG	1 cut(s)	153
MspCI	CTTAAG	2 cut(s)	7, 485
MwoI	GCNNNNNNNGC	2 cut(s)	22, 398
NarI	GGCGCC	1 cut(s)	245
NdeI	CATATG	1 cut(s)	397
NdeII	GATC	3 cut(s)	130, 289, 481
NlaIII	CATG	1 cut(s)	191
NlaIV	GGNNCC	7 cut(s)	17, 170, 183, 184, 185, 246, 460
PleI	GAGTC	1 cut(s)	241
PluTI	GGCGCC	1 cut(s)	248
PpsI	GAGTC	1 cut(s)	241
PspN4I	GGNNCC	7 cut(s)	17, 170, 183, 184, 185, 246, 460
PspOMI	GGGCCC	1 cut(s)	182
PspPI	GGNCC	4 cut(s)	23, 182, 183, 470
PsuI	RGATCY	2 cut(s)	130, 289
RseI	CAYNNNNRTG	1 cut(s)	153
SaqAI	TTAA	7 cut(s)	8, 123, 210, 276, 405, 486, 492
Sau3AI	GATC	3 cut(s)	130, 289, 481
Sau96I	GGNCC	4 cut(s)	23, 182, 183, 470
SchI	GAGTC	1 cut(s)	242
SduI	GDGCHC	2 cut(s)	186, 524
SetI	ASST	6 cut(s)	46, 67, 174, 339, 367, 437
SfaNI	GCATC	2 cut(s)	400, 487
SfoI	GGCGCC	1 cut(s)	246
SinI	GGWCC	1 cut(s)	470
SmiMI	CAYNNNNRTG	1 cut(s)	153
SmlI	CTYRAG	3 cut(s)	7, 230, 485
SmoI	CTYRAG	3 cut(s)	7, 230, 485
Sse9I	AATT	7 cut(s)	101, 108, 198, 273, 314, 441, 477
SsiI	CCGC	3 cut(s)	36, 81, 179
SspDI	GGCGCC	1 cut(s)	244
SspI	AATATT	1 cut(s)	208
SspMI	CTAG	2 cut(s)	27, 356
StyI	CCWWGG	2 cut(s)	248, 502
TaqI	TCGA	1 cut(s)	225
TasI	AATT	7 cut(s)	101, 108, 198, 273, 314, 441, 477
Tru1I	TTAA	7 cut(s)	8, 123, 210, 276, 405, 486, 492
Tru9I	TTAA	7 cut(s)	8, 123, 210, 276, 405, 486, 492
TscAI	CASTG	2 cut(s)	94, 524
TspDTI	ATGAA	4 cut(s)	75, 174, 285, 462
TspRI	CASTG	2 cut(s)	94, 524
Vha464I	CTTAAG	2 cut(s)	7, 485
VpaK11BI	GGWCC	1 cut(s)	470
XapI	RAATTY	1 cut(s)	108
XspI	CTAG	2 cut(s)	27, 356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.