Rmu_sc0000016.1_g000006

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000016.1
OG0004989
Physical Location & Seq
Forward (+)
20699 .. 26539
5841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000016.1_g000006.1.cds

Sequence Viewer

Length: 555 bp
atgctgagggtctgtggcatttggggtaaagatttaagaggcctgtggagtaaaggaactagagtggaggagaaaatgaaatctgatttgcctcttgatttgcagttttccagttgtcacccaagcattcaagatgtgaattgtatgttcaggttatttgagttgccgaatggctatgactttgactatgatgaataccacaggtttcaaacgaaacagatgtatcgatttggttggtttagcatgaagctcaagttggttagaggtgactctgttggcgttgtgacagcttactatgttcggggtggtggggggtgtgacttgcgatgccaattgctcggaggggagatgggttcccatctcttcatatcgacgaaggttgagaccagattcgacctcatgctgctgggttgggtgaggccggccaccatgggcggcgacggcgctggcggtgacaacatggccgacggtcatgttatggacagtggctccatgcctaatggggtccgatcccattcttctgtcattctttttctctctatggggataaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

184

Amino Acids

20.61

Weight (kDa)

7.66

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 435, 450
AclWI GGATC 1 cut(s) 504
AcoI YGGCCR 2 cut(s) 423, 462
AgsI TTSAA 2 cut(s) 131, 209
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 2 cut(s) 250, 290
AluI AGCT 2 cut(s) 250, 290
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 1 cut(s) 504
AoxI GGCC 4 cut(s) 40, 419, 423, 462
ApeKI GCWGC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 4 cut(s) 110, 278, 427, 464
AvaII GGWCC 1 cut(s) 505
BaeI ACNNNNGTAYC 2 cut(s) 206, 239
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 1 cut(s) 390
BccI CCATC 2 cut(s) 343, 366
BceAI ACGGC 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 447
BisI GCNGC 2 cut(s) 404, 436
BlsI GCNGC 2 cut(s) 405, 437
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 3 cut(s) 355, 490, 506
BmsI GCATC 1 cut(s) 317
Bpu10I CCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 236
Bsa29I ATCGAT 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 406
BshFI GGCC 4 cut(s) 42, 421, 425, 464
BshVI ATCGAT 1 cut(s) 226
BsiSI CCGG 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 4 cut(s) 42, 421, 425, 464
Bso31I GGTCTC 1 cut(s) 377
Bsp143I GATC 1 cut(s) 509
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 2 cut(s) 435, 450
BspANI GGCC 4 cut(s) 42, 421, 425, 464
BspDI ATCGAT 1 cut(s) 226
BspLI GGNNCC 3 cut(s) 355, 490, 506
BspPI GGATC 1 cut(s) 504
BspTNI GGTCTC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 1 cut(s) 509
BssT1I CCWWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 470, 485
Bst6I CTCTTC 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 423, 448
BstDEI CTNAG 1 cut(s) 5
BstDSI CCRYGG 1 cut(s) 429
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 1 cut(s) 512
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 1 cut(s) 509
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 226
BsuRI GGCC 4 cut(s) 42, 421, 425, 464
BsuTUI ATCGAT 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 423, 448
CfoI GCGC 1 cut(s) 446
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 505
ClaI ATCGAT 1 cut(s) 226
CviAII CATG 6 cut(s) 244, 400, 430, 460, 473, 493
CviJI RGCY 8 cut(s) 42, 174, 250, 290, 421, 425, 464, 489
CviKI_1 RGCY 8 cut(s) 42, 174, 250, 290, 421, 425, 464, 489
DdeI CTNAG 1 cut(s) 5
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
EaeI YGGCCR 2 cut(s) 423, 462
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 6 cut(s) 247, 403, 433, 463, 476, 496
FatI CATG 6 cut(s) 243, 399, 429, 459, 472, 492
Fnu4HI GCNGC 2 cut(s) 404, 436
FseI GGCCGGCC 1 cut(s) 425
Fsp4HI GCNGC 2 cut(s) 404, 436
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 445
GluI GCNGC 2 cut(s) 404, 436
GsaI CCCAGC 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 4 cut(s) 42, 421, 425, 464
HapII CCGG 1 cut(s) 422
HhaI GCGC 1 cut(s) 446
Hin1II CATG 6 cut(s) 247, 403, 433, 463, 476, 496
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HinfI GANTC 2 cut(s) 269, 390
HpaII CCGG 1 cut(s) 422
HphI GGTGA 4 cut(s) 110, 278, 427, 464
Hpy188I TCNGA 3 cut(s) 85, 341, 509
Hpy188III TCNNGA 2 cut(s) 95, 131
Hpy99I CGWCG 3 cut(s) 376, 443, 470
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 470, 485
HpyCH4V TGCA 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpyF3I CTNAG 1 cut(s) 5
Hsp92II CATG 6 cut(s) 247, 403, 433, 463, 476, 496
HspAI GCGC 1 cut(s) 444
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Kzo9I GATC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 494
LpnPI CCDG 8 cut(s) 56, 124, 136, 187, 392, 400, 432, 435
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 5 cut(s) 116, 266, 283, 317, 452
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 2 cut(s) 355, 510
MfeI CAATTG 1 cut(s) 332
MluCI AATT 2 cut(s) 139, 332
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 7 cut(s) 32, 61, 102, 257, 335, 407, 411
MroNI GCCGGC 1 cut(s) 421
MseI TTAA 1 cut(s) 35
MspI CCGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 332
Mva1269I GAATGC 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 441
NaeI GCCGGC 1 cut(s) 423
NcoI CCATGG 1 cut(s) 429
NdeII GATC 1 cut(s) 509
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 6 cut(s) 247, 403, 433, 463, 476, 496
NlaIV GGNNCC 3 cut(s) 355, 490, 506
NmuCI GTSAC 5 cut(s) 116, 266, 283, 317, 452
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 390
PkrI GCNGC 2 cut(s) 405, 437
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 406
PspN4I GGNNCC 3 cut(s) 355, 490, 506
PspPI GGNCC 1 cut(s) 505
RigI GGCCGGCC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 2 cut(s) 404, 436
Sau3AI GATC 1 cut(s) 509
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 1 cut(s) 263
SetI ASST 7 cut(s) 155, 206, 252, 268, 292, 381, 399
SfaNI GCATC 1 cut(s) 317
SinI GGWCC 1 cut(s) 505
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 139, 332
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 2 cut(s) 435, 450
SspMI CTAG 1 cut(s) 60
StuI AGGCCT 1 cut(s) 42
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 2 cut(s) 470, 485
TaqI TCGA 3 cut(s) 226, 371, 393
TasI AATT 2 cut(s) 139, 332
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 5 cut(s) 116, 266, 283, 317, 452
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 5 cut(s) 116, 266, 283, 317, 452
TspDTI ATGAA 4 cut(s) 92, 207, 260, 355
TspRI CASTG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 505
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.