Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

27686784 .. 27703509

Gene Structure 16726 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1247900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3444 bp

ATGGCTACCGACGAGACCAAGTTCAGCGTGCTCTGCAGCCTCTTCAACTGGATGCAGAAATCCAAAACCTCAACCCTAAAGCGTTCCAAATTCCGCAGATTTCTCGACAATTTCTGCAAACCCGCCAATTACTTCAGCGCCATTCGCCTCATTCTTCCCAGCCTCGACCGCGAGCGTGGCTCCTACGGCCTCAAGGAGTCCGTGCTTGCCACCTGCCTCATCGACGCCCTCGGCATCGCCAGAGACTCCGAGGACGCTCTCCGCCTCATTAATTGGCGCAAAGGTGGCGCCAAGACCGGCGCCAATGCCGGGAACTTCTCCCTCGTGGCGGCGGAGGTGTTGCAACGTAGACAGGGGTTGAGTTCAGGTGGACTAACAATCAAGGAGTTAAATGACTTGCTTGATCGTTTAGCTTCGAGTGAAAACAGGGCAGAGAAGACTTCAGTTCTTTCAACCTTGATAAACAAGACAAATGCACAGGAAATGAAGTGGATTGTCATGATCATTCTTAAAGATCTGAAGCTGGGAACTAGTGAAAAGAGCATCTTTCACGAATTTCATCCAGATGCTGAAGATTTGTTTAATGTCACATGCGACTTGAAATTGGTCTGTGAAAAGCTCAGTGATAGAAATCAGCGCCATAAACGCCAGGACATTGAAGTTGGAAAAGCTGTGCGCCCACAACTAGCTATGAGAGTTGGTGATGCAAATATTGCATGGAAAAAGTTTCATGGTAAGGAAGTGGTTGTTGAGTGTAAATTCGATGGCGATCGCATCCAAATTCATAAGAACGGAACTGAAGTACATTACTTCTCAAGGAATTTTCTTGAGCACTCTGAATACACACATGCAATGTCAGACATTATCGTAGAAAATGTGCTGGCTGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTTCTAGTCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGATGTTGCATTCGATATCCTCTATGTTGGAGATACTAGTGTGATTCACCAGAGCTTGAAGGAACGACATGAGCTTCTCCAGAAAGTTGTGAATCCATTGAAGGGTTGTTTAGAGATTTTAGTACCCAATCGTGGTCTTAACGCTCACTGTAATGAAGGGGAACCTTGCTGGTCGCTTATTGCTACTAGTGTGGATGATGTTGAGAAGTTTTTCAAAGATACCATCGAAAATAGAGATGAGGGGATTGTTTTGAAGGACCTTGGTTCGAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTAAAATTAAAGCCAGACTACATTCGTGCAGGTTCTGATCTGGACGTTCTTATCATAGGAGGATACTATGGCTCTGGACGTCGTGGAGGAGTGGTAGCTCAGTTCTTGGTGGGTCTTGCTGAGCGTCCAGTTCCAAATACATATCCTAGAAGGTTTGTATCCTTTTGCAGAGTGGGCACTGGACTTTCTGATGAAGAGATAGAAGCTGTTGCAACAAAATTAAAGCCGTATTTGAGGAAATATGAGTACCCAAAAAAGGCACCACCAAGTTTTTATCAAGTAACAAACAATTCTAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATACTTTCTATAACAAGTGACATCAGAACTATAAAGTCTGAGGTTTTTGCTGCACCATACAGCCTGAGATTTCCACGTATTGATCGTGTTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAGTCATTTATAGAGCTTGTACAAGCAGGCAATGGGACCACACAAAGGGGGACGGATTATGGTGGTCTGCAGGATACTAAAAAGAAAAGCAAGAAGTCCCCTAGAAAGGAAGAGAAAAGAAATCTGTCTATTGTCCCCTCTCATTTTGTTCAGACTGATGTTTCGGGCGTGAAGGAGGACACATTACTATTTTCAAATATGATGTTTTACTTTGTTAATGTGCCTCCCACTCATTCTTTTGATTCCTTGCACAAGCTAGTTGTTGAGAATGGGGGTACTTTCTCTATGAATTTAAATAATGCTGTTACACACTGTGTTGCAGCTGATGGCAAAGGGATCAAGTATCAGGCAGCAAAGCATCATGGAGATATCATTCATATCTCTTGGGTGTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCATTACAGCCAAAGTACTATCTCTTCTTATCTGACTCTTCTAAGAAGTTACAAGAAGAGATTGATGATTTTTCTGACTCGTACTACTGGGATCTTGACCTTGCGGAGATAAAGCAGCTTTTAATCAACATTCATAGATCAGAGGACTCCGAAGTTGTTAACTATTACAAGAAAAAATACTGTCCGGTGGAGAGATGGTCTCGCTTTCATGACTGCTGCATTTACTTTAAATCCTCAATTCATTCCTTGAAGCCGGATTGGGAAGTTGTCTTAGGATTCACACTGAGAAGGTTGAGGATTGAAGTTCTCAGGGGTGGTGGCAGAGTCACTGACAGTCTTGTTCATGCGACCCATTTGGTAGTCTTGTCCGTGCAGGGTCCTGATGGATTTGAAACTTTATTACGAAGTTTCACTGAAGCAGACAAACGATATCTGCATAGCAAAAGGTTGCATATTGTTGGATCTCAATGGTTGGAAGACTGCTTGGAAAGGGACCAAAGATTGCAAGAGGAAACATATAGCTTAAAACCCTATGGGTTGGAAGAATCCAACGTTGAAGAATGGAAACATGACATGGGTCTAGAAGAAGCACCCTGTGATGTGGATGCTGTGGAAAACCACAGTTCTTCTTTTCCTAACCGGAAAGGAAAAGAGATTGAAGTAAAAGCGGCTCCAGAAAACTGTAAGATCTTGGACTCACCCGAGAGGGGAAGCAAAAGGAAAAGGGGAAGACCTGCTCGCACAAGCTTGAACAAAGGAAAATCAACTGTCAACCAAGCTAGAAGAGCATGGGCGCGTATTGGAAGGAAGCCAGCTAAAATATCTGGCAACGAGTCAGAAGAGAGTGGCTCTCATGACAATACATCACTTGGGGAAGAGGTTGATCTGGGAGAAAGAATTCACGGAACGGTAGGTCAGGAAAGTATAGACATCCAAGAAAATGAAGCAATGAAAGATTCTGAATTGTTACAAAGAGGCAAAGCAGTTGAGCAAGACGTCGCTGAGGACATTAGAATTGAAGAGCCTTACAAAATTCCTGAAATCAAAATGATGGAGAAACATAACGTCCAAGAAAGTGAAAAGCCGGAGGAACTTGAAGCTACGGCTGATCCTGTTGAAGCCATGTTGTTTGACATGATTCCAAGTCTTGGTATGAAAAAAGCTGAAACTACATATACGAGTACTCGAGATGAGAAACCAGCACGGGATCCGATACCGAATGCAGGACCTTCAAAGAAAAAGAAAGTTAGCTACAAGGATATTGCTAGTGAGCTGCTGAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1148

Amino Acids

130.31

Weight (kDa)

6.56

Isoelectric Point (pI)

44.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	7 - 187	5.8e-38	DNA ligase N terminus
DNA_ligase_A_M	PF01068	227 - 427	2.5e-34	ATP dependent DNA ligase domain
RNA_ligase	PF09414	246 - 426	1e-06	RNA ligase
DNA_ligase_A_C	PF04679	452 - 570	2.4e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	640 - 711	1.1e-07	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	641 - 722	1.7e-12	BRCT domain
PTCB-BRCT	PF12738	659 - 700	3.3e-08	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	221
AatII	GACGTC	2 cut(s)	1351, 3153
Acc36I	ACCTGC	3 cut(s)	221, 1289, 2896
AccB1I	GGYRCC	3 cut(s)	287, 299, 1528
AccB7I	CCANNNNNTGG	1 cut(s)	3302
AccI	GTMKAC	1 cut(s)	349
AccII	CGCG	2 cut(s)	171, 2950
AciI	CCGC	8 cut(s)	94, 123, 169, 262, 329, 332, 2258, 2822
AclI	AACGTT	1 cut(s)	2706
AclWI	GGATC	6 cut(s)	2078, 2253, 2623, 3257, 3356, 3369
AcsI	RAATTY	8 cut(s)	89, 554, 758, 780, 820, 2023, 3051, 3186
AcuI	CTGAAG	6 cut(s)	118, 426, 539, 591, 819, 2589
AcyI	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
AdeI	CACNNNGTG	2 cut(s)	2048, 2750
AfaI	GTAC	8 cut(s)	804, 1092, 1517, 1755, 2011, 2171, 2237, 3337
AhlI	ACTAGT	3 cut(s)	530, 1004, 1154
AjnI	CCWGG	1 cut(s)	648
Alw21I	GWGCWC	2 cut(s)	33, 834
Alw26I	GTCTC	4 cut(s)	8, 237, 1564, 2358
AlwI	GGATC	6 cut(s)	2078, 2253, 2623, 3257, 3356, 3369
AlwNI	CAGNNNCTG	2 cut(s)	569, 1304
Ama87I	CYCGRG	2 cut(s)	2855, 3339
AoxI	GGCC	1 cut(s)	187
ApeKI	GCWGC	7 cut(s)	36, 1658, 2054, 2084, 2269, 2370, 3427
ApoI	RAATTY	8 cut(s)	89, 554, 758, 780, 820, 2023, 3051, 3186
ArsI	GACNNNNNNTTYG	4 cut(s)	1563, 1595, 1879, 1911
AseI	ATTAAT	1 cut(s)	270
AsiSI	GCGATCGC	1 cut(s)	772
Asp700I	GAANNNNTTC	2 cut(s)	1178, 3051
AspLEI	GCGC	7 cut(s)	140, 279, 290, 302, 639, 678, 2950
AspS9I	GGNCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
AsuC2I	CCSGG	1 cut(s)	310
AsuHPI	GGTGA	4 cut(s)	713, 917, 1007, 2844
AsuII	TTCGAA	1 cut(s)	1235
AvaI	CYCGRG	2 cut(s)	2855, 3339
AvaII	GGWCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
BaeGI	GKGCMC	1 cut(s)	1448
BaeI	ACNNNNGTAYC	2 cut(s)	993, 1026
BamHI	GGATCC	1 cut(s)	3361
BanI	GGYRCC	3 cut(s)	287, 299, 1528
BarI	GAAGNNNNNNTAC	2 cut(s)	2162, 2194
BauI	CACGAG	1 cut(s)	323
BbsI	GAAGAC	3 cut(s)	443, 2637, 2890
Bbv12I	GWGCWC	2 cut(s)	33, 834
BbvCI	CCTCAGC	1 cut(s)	3156
BbvI	GCAGC	7 cut(s)	48, 1645, 2066, 2096, 2281, 2357, 3414
BccI	CCATC	7 cut(s)	758, 896, 1199, 2054, 2343, 2531, 3199
BceAI	ACGGC	3 cut(s)	202, 1480, 3273
BcgI	CGANNNNNNTGC	2 cut(s)	85, 119
BciT130I	CCWGG	1 cut(s)	650
BciVI	GTATCC	3 cut(s)	1325, 1438, 1801
BclI	TGATCA	1 cut(s)	501
BcnI	CCSGG	1 cut(s)	310
BcoDI	GTCTC	4 cut(s)	8, 237, 1564, 2358
BcuI	ACTAGT	3 cut(s)	530, 1004, 1154
BfmI	CTRYAG	2 cut(s)	34, 1802
BfoI	RGCGCY	4 cut(s)	141, 291, 303, 640
BfuAI	ACCTGC	3 cut(s)	221, 1289, 2896
BfuI	GTATCC	3 cut(s)	1325, 1438, 1801
BglII	AGATCT	2 cut(s)	514, 2841
BisI	GCNGC	9 cut(s)	37, 330, 1659, 2055, 2085, 2270, 2371, 2823, 3428
BlpI	GCTNAGC	1 cut(s)	1389
BlsI	GCNGC	9 cut(s)	38, 331, 1660, 2056, 2086, 2271, 2372, 2824, 3429
BmcAI	AGTACT	2 cut(s)	2171, 3337
Bme1390I	CCNGG	2 cut(s)	310, 650
Bme18I	GGWCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
BmeT110I	CYCGRG	2 cut(s)	2855, 3339
BmgT120I	GGNCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
BmrFI	CCNGG	2 cut(s)	310, 650
BmrI	ACTGGG	1 cut(s)	2251
BmsI	GCATC	8 cut(s)	42, 243, 552, 556, 694, 783, 2101, 2749
BmuI	ACTGGG	1 cut(s)	2251
BoxI	GACNNNNGTC	1 cut(s)	2730
BpiI	GAAGAC	3 cut(s)	443, 2637, 2890
BplI	GAGNNNNNCTC	8 cut(s)	164, 196, 2338, 2370, 2987, 3019, 2989, 3021
BpmI	CTGGAG	2 cut(s)	1031, 2811
Bpu10I	CCTNAGC	1 cut(s)	3156
Bpu1102I	GCTNAGC	1 cut(s)	1389
Bpu14I	TTCGAA	1 cut(s)	1235
BpuEI	CTTGAG	3 cut(s)	176, 799, 848
BpuMI	CCSGG	1 cut(s)	310
BsaAI	YACGTR	1 cut(s)	1685
BsaBI	GATNNNNATC	2 cut(s)	630, 768
BsaHI	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
BsaI	GGTCTC	3 cut(s)	8, 1564, 2358
BsaJI	CCNNGG	4 cut(s)	229, 249, 1228, 1712
BsaWI	WCCGGW	2 cut(s)	2338, 2793
BsaXI	ACNNNNNCTCC	4 cut(s)	1347, 1377, 2336, 2366
Bse118I	RCCGGY	1 cut(s)	296
Bse1I	ACTGG	4 cut(s)	53, 1397, 1453, 2246
Bse3DI	GCAATG	3 cut(s)	858, 1771, 3108
Bse8I	GATNNNNATC	2 cut(s)	630, 768
BseBI	CCWGG	1 cut(s)	650
BseDI	CCNNGG	4 cut(s)	229, 249, 1228, 1712
BseGI	GGATG	6 cut(s)	57, 559, 774, 1168, 2764, 3084
BseJI	GATNNNNATC	2 cut(s)	630, 768
BseMI	GCAATG	3 cut(s)	858, 1771, 3108
BseNI	ACTGG	4 cut(s)	53, 1397, 1453, 2246
BseRI	GAGGAG	1 cut(s)	1371
BseSI	GKGCMC	1 cut(s)	1448
BseXI	GCAGC	7 cut(s)	48, 1645, 2066, 2096, 2281, 2357, 3414
BseYI	CCCAGC	2 cut(s)	158, 523
BsgI	GTGCAG	4 cut(s)	1317, 1644, 1754, 2546
Bsh1236I	CGCG	2 cut(s)	171, 2950
Bsh1285I	CGRYCG	2 cut(s)	169, 772
BshFI	GGCC	1 cut(s)	189
BshNI	GGYRCC	3 cut(s)	287, 299, 1528
BsiEI	CGRYCG	2 cut(s)	169, 772
BsiHKAI	GWGCWC	2 cut(s)	33, 834
BsiHKCI	CYCGRG	2 cut(s)	2855, 3339
BsiSI	CCGG	6 cut(s)	297, 309, 2339, 2408, 2794, 3239
BslFI	GGGAC	6 cut(s)	935, 1783, 1798, 1816, 1853, 2660
BsmAI	GTCTC	4 cut(s)	8, 237, 1564, 2358
BsmFI	GGGAC	6 cut(s)	935, 1783, 1798, 1816, 1853, 2660
BsmI	GAATGC	3 cut(s)	977, 1727, 3379
BsnI	GGCC	1 cut(s)	189
Bso31I	GGTCTC	3 cut(s)	8, 1564, 2358
BsoBI	CYCGRG	2 cut(s)	2855, 3339
Bsp119I	TTCGAA	1 cut(s)	1235
Bsp1286I	GDGCHC	3 cut(s)	33, 834, 1448
Bsp1407I	TGTACA	1 cut(s)	1753
Bsp1720I	GCTNAGC	1 cut(s)	1389
BspACI	CCGC	8 cut(s)	94, 123, 169, 262, 329, 332, 2258, 2822
BspANI	GGCC	1 cut(s)	189
BspFNI	CGCG	2 cut(s)	171, 2950
BspHI	TCATGA	3 cut(s)	498, 2362, 3007
BspMAI	CTGCAG	2 cut(s)	38, 1806
BspMI	ACCTGC	3 cut(s)	221, 1289, 2896
BspPI	GGATC	6 cut(s)	2078, 2253, 2623, 3257, 3356, 3369
BspQI	GCTCTTC	2 cut(s)	2932, 3168
BspT104I	TTCGAA	1 cut(s)	1235
BspT107I	GGYRCC	3 cut(s)	287, 299, 1528
BspTNI	GGTCTC	3 cut(s)	8, 1564, 2358
BsrDI	GCAATG	3 cut(s)	858, 1771, 3108
BsrFI	RCCGGY	1 cut(s)	296
BsrGI	TGTACA	1 cut(s)	1753
BsrI	ACTGG	4 cut(s)	53, 1397, 1453, 2246
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	4 cut(s)	229, 249, 1228, 1712
BssNI	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	2 cut(s)	1228, 1712
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	1 cut(s)	650
Bst4CI	ACNGT	8 cut(s)	1118, 2048, 2336, 2489, 2777, 2837, 2923, 3064
BstACI	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
BstAPI	GCANNNNNTGC	1 cut(s)	713
BstAUI	TGTACA	1 cut(s)	1753
BstBAI	YACGTR	1 cut(s)	1685
BstBI	TTCGAA	1 cut(s)	1235
BstC8I	GCNNGC	7 cut(s)	29, 173, 207, 882, 1762, 2893, 2967
BstF5I	GGATG	6 cut(s)	57, 559, 774, 1168, 2764, 3084
BstFNI	CGCG	2 cut(s)	171, 2950
BstH2I	RGCGCY	4 cut(s)	141, 291, 303, 640
BstHHI	GCGC	7 cut(s)	140, 279, 290, 302, 639, 678, 2950
BstMAI	GTCTC	4 cut(s)	8, 237, 1564, 2358
BstMCI	CGRYCG	2 cut(s)	169, 772
BstNI	CCWGG	1 cut(s)	650
BstNSI	RCATGY	2 cut(s)	594, 851
BstPAI	GACNNNNGTC	1 cut(s)	2730
BstSCI	CCNGG	2 cut(s)	308, 648
BstSFI	CTRYAG	2 cut(s)	34, 1802
BstSLI	GKGCMC	1 cut(s)	1448
BstUI	CGCG	2 cut(s)	171, 2950
BstV1I	GCAGC	7 cut(s)	48, 1645, 2066, 2096, 2281, 2357, 3414
BstV2I	GAAGAC	3 cut(s)	443, 2637, 2890
BstX2I	RGATCY	5 cut(s)	514, 2245, 2615, 2841, 3361
BstXI	CCANNNNNNTGG	1 cut(s)	1581
BstYI	RGATCY	5 cut(s)	514, 2245, 2615, 2841, 3361
BsuI	GTATCC	3 cut(s)	1325, 1438, 1801
BsuRI	GGCC	1 cut(s)	189
BtgZI	GCGATG	1 cut(s)	220
BtsCI	GGATG	6 cut(s)	57, 559, 774, 1168, 2764, 3084
BtsIMutI	CAGTG	7 cut(s)	628, 1114, 1446, 2044, 2435, 2481, 2565
BveI	ACCTGC	3 cut(s)	221, 1289, 2896
Cac8I	GCNNGC	7 cut(s)	29, 173, 207, 882, 1762, 2893, 2967
CaiI	CAGNNNCTG	2 cut(s)	569, 1304
CciI	TCATGA	3 cut(s)	498, 2362, 3007
CfoI	GCGC	7 cut(s)	140, 279, 290, 302, 639, 678, 2950
Cfr10I	RCCGGY	1 cut(s)	296
Cfr13I	GGNCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
CseI	GACGC	3 cut(s)	233, 263, 1382
Csp6I	GTAC	8 cut(s)	803, 1091, 1516, 1754, 2010, 2170, 2236, 3336
CviQI	GTAC	8 cut(s)	803, 1091, 1516, 1754, 2010, 2170, 2236, 3336
DinI	GGCGCC	2 cut(s)	289, 301
DraI	TTTAAA	2 cut(s)	2028, 2383
DraIII	CACNNNGTG	2 cut(s)	2048, 2750
EciI	GGCGGA	2 cut(s)	251, 347
Eco130I	CCWWGG	2 cut(s)	1228, 1712
Eco31I	GGTCTC	3 cut(s)	8, 1564, 2358
Eco32I	GATATC	3 cut(s)	985, 2104, 2585
Eco47I	GGWCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
Eco57I	CTGAAG	6 cut(s)	118, 426, 539, 591, 819, 2589
Eco88I	CYCGRG	2 cut(s)	2855, 3339
EcoO109I	RGGNCCY	3 cut(s)	1225, 2531, 3380
EcoRI	GAATTC	1 cut(s)	3051
EcoRII	CCWGG	1 cut(s)	648
EcoRV	GATATC	3 cut(s)	985, 2104, 2585
EcoT14I	CCWWGG	2 cut(s)	1228, 1712
EgeI	GGCGCC	2 cut(s)	289, 301
EheI	GGCGCC	2 cut(s)	289, 301
ErhI	CCWWGG	2 cut(s)	1228, 1712
FalI	AAGNNNNNCTT	2 cut(s)	530, 562
FaqI	GGGAC	6 cut(s)	935, 1783, 1798, 1816, 1853, 2660
FauI	CCCGC	1 cut(s)	130
FbaI	TGATCA	1 cut(s)	501
FblI	GTMKAC	1 cut(s)	349
Fnu4HI	GCNGC	9 cut(s)	37, 330, 1659, 2055, 2085, 2270, 2371, 2823, 3428
FokI	GGATG	6 cut(s)	64, 546, 761, 1175, 2771, 3071
Fsp4HI	GCNGC	9 cut(s)	37, 330, 1659, 2055, 2085, 2270, 2371, 2823, 3428
GlaI	GCGC	7 cut(s)	139, 278, 289, 301, 638, 677, 2949
GluI	GCNGC	9 cut(s)	37, 330, 1659, 2055, 2085, 2270, 2371, 2823, 3428
GsaI	CCCAGC	2 cut(s)	162, 527
GsuI	CTGGAG	2 cut(s)	1031, 2811
HaeII	RGCGCY	4 cut(s)	141, 291, 303, 640
HaeIII	GGCC	1 cut(s)	189
HapII	CCGG	6 cut(s)	297, 309, 2339, 2408, 2794, 3239
HgaI	GACGC	3 cut(s)	233, 263, 1382
HhaI	GCGC	7 cut(s)	140, 279, 290, 302, 639, 678, 2950
Hin1I	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
Hin6I	GCGC	7 cut(s)	138, 277, 288, 300, 637, 676, 2948
HinP1I	GCGC	7 cut(s)	138, 277, 288, 300, 637, 676, 2948
HincII	GTYRAC	2 cut(s)	2314, 2926
HindII	GTYRAC	2 cut(s)	2314, 2926
HindIII	AAGCTT	1 cut(s)	2899
HpaI	GTTAAC	1 cut(s)	2314
HpaII	CCGG	6 cut(s)	297, 309, 2339, 2408, 2794, 3239
HphI	GGTGA	4 cut(s)	713, 917, 1007, 2844
Hpy166II	GTNNAC	4 cut(s)	350, 371, 2314, 2926
Hpy8I	GTNNAC	4 cut(s)	350, 371, 2314, 2926
Hpy99I	CGWCG	4 cut(s)	14, 227, 1353, 3155
HpyCH4III	ACNGT	8 cut(s)	1118, 2048, 2336, 2489, 2777, 2837, 2923, 3064
HpyCH4IV	ACGT	7 cut(s)	346, 1314, 1348, 1684, 2706, 3150, 3219
HpySE526I	ACGT	7 cut(s)	346, 1314, 1348, 1684, 2706, 3150, 3219
Hsp92I	GRCGYC	5 cut(s)	225, 288, 300, 1348, 3150
HspAI	GCGC	7 cut(s)	138, 277, 288, 300, 637, 676, 2948
KasI	GGCGCC	2 cut(s)	287, 299
Ksp22I	TGATCA	1 cut(s)	501
KspAI	GTTAAC	1 cut(s)	2314
LguI	GCTCTTC	2 cut(s)	2932, 3168
LmnI	GCTCC	2 cut(s)	185, 2830
Lsp1109I	GCAGC	7 cut(s)	48, 1645, 2066, 2096, 2281, 2357, 3414
LweI	GCATC	8 cut(s)	42, 243, 552, 556, 694, 783, 2101, 2749
MaeII	ACGT	7 cut(s)	346, 1314, 1348, 1684, 2706, 3150, 3219
MaeIII	GTNAC	7 cut(s)	586, 1549, 1625, 2038, 2202, 2479, 3120
MflI	RGATCY	5 cut(s)	514, 2245, 2615, 2841, 3361
MhlI	GDGCHC	3 cut(s)	33, 834, 1448
Mly113I	GGCGCC	2 cut(s)	288, 300
MlyI	GAGTC	8 cut(s)	206, 239, 2183, 2225, 2294, 2487, 2843, 2996
MmeI	TCCRAC	7 cut(s)	643, 976, 2106, 2593, 2607, 2673, 2727
MroXI	GAANNNNTTC	2 cut(s)	1178, 3051
MslI	CAYNNNNRTG	2 cut(s)	564, 1119
MspA1I	CMGCKG	1 cut(s)	2057
MspI	CCGG	6 cut(s)	297, 309, 2339, 2408, 2794, 3239
MspR9I	CCNGG	2 cut(s)	310, 650
Mva1269I	GAATGC	3 cut(s)	977, 1727, 3379
MvaI	CCWGG	1 cut(s)	650
MvnI	CGCG	2 cut(s)	171, 2950
NarI	GGCGCC	2 cut(s)	288, 300
NciI	CCSGG	1 cut(s)	310
NmeAIII	GCCGAG	1 cut(s)	210
NmuCI	GTSAC	3 cut(s)	586, 1625, 2479
NspI	RCATGY	2 cut(s)	594, 851
NspV	TTCGAA	1 cut(s)	1235
PaeR7I	CTCGAG	1 cut(s)	3339
PagI	TCATGA	3 cut(s)	498, 2362, 3007
PaqCI	CACCTGC	1 cut(s)	221
PciSI	GCTCTTC	2 cut(s)	2932, 3168
PcsI	WCGNNNNNNNCGW	1 cut(s)	1690
PctI	GAATGC	3 cut(s)	977, 1727, 3379
PdmI	GAANNNNTTC	2 cut(s)	1178, 3051
PfeI	GAWTC	7 cut(s)	1012, 1060, 1976, 2430, 2699, 3110, 3292
PflMI	CCANNNNNTGG	1 cut(s)	3302
PkrI	GCNGC	9 cut(s)	38, 331, 1660, 2056, 2086, 2271, 2372, 2824, 3429
Ple19I	CGATCG	1 cut(s)	772
PleI	GAGTC	8 cut(s)	205, 239, 2183, 2225, 2294, 2486, 2843, 2995
PluTI	GGCGCC	2 cut(s)	291, 303
PpsI	GAGTC	8 cut(s)	205, 239, 2183, 2225, 2294, 2486, 2843, 2995
Ppu21I	YACGTR	1 cut(s)	1685
PpuMI	RGGWCCY	3 cut(s)	1225, 2531, 3380
PshAI	GACNNNNGTC	1 cut(s)	2730
PshBI	ATTAAT	1 cut(s)	270
Psp1406I	AACGTT	1 cut(s)	2706
Psp5II	RGGWCCY	3 cut(s)	1225, 2531, 3380
Psp6I	CCWGG	1 cut(s)	648
PspFI	CCCAGC	2 cut(s)	158, 523
PspGI	CCWGG	1 cut(s)	648
PspPI	GGNCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
PspPPI	RGGWCCY	3 cut(s)	1225, 2531, 3380
PstI	CTGCAG	2 cut(s)	38, 1806
PstNI	CAGNNNCTG	2 cut(s)	569, 1304
PsuI	RGATCY	5 cut(s)	514, 2245, 2615, 2841, 3361
PvuI	CGATCG	1 cut(s)	772
PvuII	CAGCTG	1 cut(s)	2057
RgaI	GCGATCGC	1 cut(s)	772
RsaI	GTAC	8 cut(s)	804, 1092, 1517, 1755, 2011, 2171, 2237, 3337
RsaNI	GTAC	8 cut(s)	803, 1091, 1516, 1754, 2010, 2170, 2236, 3336
RseI	CAYNNNNRTG	2 cut(s)	564, 1119
SapI	GCTCTTC	2 cut(s)	2932, 3168
SatI	GCNGC	9 cut(s)	37, 330, 1659, 2055, 2085, 2270, 2371, 2823, 3428
Sau96I	GGNCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
ScaI	AGTACT	2 cut(s)	2171, 3337
SchI	GAGTC	8 cut(s)	206, 239, 2183, 2225, 2294, 2487, 2843, 2996
ScrFI	CCNGG	2 cut(s)	310, 650
SduI	GDGCHC	3 cut(s)	33, 834, 1448
SfaAI	GCGATCGC	1 cut(s)	772
SfaNI	GCATC	8 cut(s)	42, 243, 552, 556, 694, 783, 2101, 2749
SfcI	CTRYAG	2 cut(s)	34, 1802
SfoI	GGCGCC	2 cut(s)	289, 301
Sfr274I	CTCGAG	1 cut(s)	3339
SfuI	TTCGAA	1 cut(s)	1235
SgfI	GCGATCGC	1 cut(s)	772
SinI	GGWCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
SlaI	CTCGAG	1 cut(s)	3339
SmiI	ATTTAAAT	1 cut(s)	2028
SmiMI	CAYNNNNRTG	2 cut(s)	564, 1119
SmlI	CTYRAG	4 cut(s)	191, 814, 827, 3339
SmoI	CTYRAG	4 cut(s)	191, 814, 827, 3339
SpeI	ACTAGT	3 cut(s)	530, 1004, 1154
SsiI	CCGC	8 cut(s)	94, 123, 169, 262, 329, 332, 2258, 2822
SspDI	GGCGCC	2 cut(s)	287, 299
SspI	AATATT	1 cut(s)	712
StyD4I	CCNGG	2 cut(s)	308, 648
StyI	CCWWGG	2 cut(s)	1228, 1712
SwaI	ATTTAAAT	1 cut(s)	2028
TaaI	ACNGT	8 cut(s)	1118, 2048, 2336, 2489, 2777, 2837, 2923, 3064
TaiI	ACGT	7 cut(s)	349, 1317, 1351, 1687, 2709, 3153, 3222
TaqI	TCGA	9 cut(s)	105, 165, 222, 416, 762, 981, 1194, 1235, 3340
TatI	WGTACW	4 cut(s)	802, 1753, 2169, 3335
TauI	GCSGC	2 cut(s)	332, 2825
TfiI	GAWTC	7 cut(s)	1012, 1060, 1976, 2430, 2699, 3110, 3292
TscAI	CASTG	7 cut(s)	628, 1121, 1453, 2051, 2442, 2488, 2572
TseFI	GTSAC	3 cut(s)	586, 1625, 2479
TseI	GCWGC	7 cut(s)	36, 1658, 2054, 2084, 2269, 2370, 3427
Tsp45I	GTSAC	3 cut(s)	586, 1625, 2479
TspGWI	ACGGA	5 cut(s)	190, 807, 1802, 2512, 3072
TspRI	CASTG	7 cut(s)	628, 1121, 1453, 2051, 2442, 2488, 2572
Van91I	CCANNNNNTGG	1 cut(s)	3302
VpaK11BI	GGWCC	5 cut(s)	1225, 1770, 2531, 2647, 3380
VspI	ATTAAT	1 cut(s)	270
XapI	RAATTY	8 cut(s)	89, 554, 758, 780, 820, 2023, 3051, 3186
XbaI	TCTAGA	1 cut(s)	2734
XceI	RCATGY	2 cut(s)	594, 851
XhoI	CTCGAG	1 cut(s)	3339
XmiI	GTMKAC	1 cut(s)	349
XmnI	GAANNNNTTC	2 cut(s)	1178, 3051
ZraI	GACGTC	2 cut(s)	1349, 3151
ZrmI	AGTACT	2 cut(s)	2171, 3337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.