Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

15725381 .. 15736908

Gene Structure 11528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG091600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3504 bp

ATGCAACGAAGCAAAACGTCAGCCCTAAAGCGCTCCAAGTTCCGGAAATTCCTTGACAACTTCTGCAAAGCAGGGGATTACTTCAGCGCAATCCGGCTGATTCTTCCGAACCTCGATCGGGAGCGCGGTAGCTATGGCCTTAAGGAGTCGGTGCTCGCCACCTGCCTCATCGACGCTCTCGGCATGTCCCGAGACTCCGACGACGCTCTTCGCCTCATCAATTGGCGCAAGGGCGGCGCCAAGACCGGCGCCAATGCCGGCAACTTCGCCCTCGTCGCCGCCGAGGTGCTGCAACGGCGACAGGGACTTAGTTCAGGTGGACTAACGATTAAGGAGTTGAATGACTTGCTTGATCGTTTGGCATCCGGTGAAAATAGGGCAGAGAAGACTTCAATTCTTTCTACTTTGGTACAGAAGACAAATGCACAAGAAATGAAGTGGATCGTCATGATCATTCTTAAAGATCTCAAATTGGGAATTAGTGAAAAGAGCATCTTCCATGAATTTCATCCGGATGCTGAAGACTTGTTTAACGTTACCTGTGACTTGAAATTGGTTTGTGAAAAGCTGAGGGACAGAAGTCAACGACATAAACGACAGGACATTGAAGTTGGAAAAGCTGTGCGCCCGCAGCTATCTATGAGAGTTGGTGATGCAACTGGTGCATGGAAAAAGTTTCATGGAAAGGAAGTTGTTGTTGAGTGTAAATTCGACGGCGATCGCATCCAAATTCATAAGAATGGAACCGAAGTACATTACTTCTCAAGGAATTTTCTTGATCACTCTGAATATGGACATGCGATGTCAGACATTATTATACAAAATGTTCAGGCCGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTCTGAAGCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCAAAGGCAGCGAGGGATGGACTTGACAGTGATAAACAGTTGTGCTATGTTGCATTCGATATCCTTTACGTTGGAGATACTAGTGTAATTCATGAGAGCTTGAAGGAAAGGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATTTTAGTACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGGGGAACCTTGTTGGTCCCTAATTGTTCATACTCTGGATGACGTCGAGAGGTTTTTTAAAGAAACCATCGAGAACAGAGATGAGGGGATTGTTTTAAAGGATCTTGGTTCTAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTGAAATTGAAGCCGGATTACATACGGGCTGGTTCTGATCTGGATGTTCTTATAATAGGAGGATACTATGGTTCAGGACGTCGTGGAGGGGAAGTAGCTCAGTTCTTGGTGGGTCTTGCTGAACGTCCAGTTGCAAATACATATCCGAGAAGGTTTGTATCCTTTTGCAGAGTGGGTACTGGACTTTCTGACGAGGAGTTAGATGCTGTGGCAACCAAATTAAAGCCTTATTTAAGGAAGTATGACTACCCCAAAAAGGCACCACCAAGTTTTTATGAAGTAATGAACCATTCAAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATCCTTTCCATAACGAGTGACATCCGAACCATAAGGTCTGAGGTTTTTGCTGCACCATACAGCCTGAGATTTCCACGTATTGATCGAATTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAATCATTTATAGAGCTTGTAGATTCAGGCAATGGTACCACACAAAAGGGGACAGATTATGGTGGCCTGCAGGATGATAAAAAGAAACACAAGAGATCCTTTAAAAAAGAAGAGAAAAGAAATCTATCTCTTGTTCCCTCTCATTTAGTTCAGACTGATGTTTCTGGTGTCAAGGAGGACAGCTTAATATTTTCAAAAATGATGTTCTACTTTGCTAATGTGCCTCCAACTAATTCTCTTGATTCCTTGCACAAAATTGTTGCTGAGAATGGGGGTACTTTTTCGATGAATTTGAATAACTCAGTCACACACTGTGTTGCAGCTGATAGCAAAGGGATTAAATATCAGGCAGCAAAACGTCAGGGAGATGTCATTCATTTGTCTTGGGTCTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCTTTACAGCCAAAGTACTACCTCTTCATTTCTGACTCTTCAAAGAAGTTAGAAGAAGAAATAGATGAGTTTTCTGACCCGTACTTCTGGGACCTTGACCTTGCAGACATTAAGCAGCTTCTAAGCAATGTGCCTAGATCAGAGGATACCAAACGTATCAACTATTACAAGAAAAAATACTGTCCTATGGAGAAATGGTCTCAATTTCACGGCTGCTGCATTTACTTTAACTCATCAATTCATTCCTTGAAACCGGATTGGGAAGTCGTGTTAGGAGTTACAGTAAGAAGGTTAAAGATTGAAGTTCTCATGGGTGGTGGCAGAGTCAGTGACAATCTTGCTGATGCCACCCATTTGGTACTCTTGTCAATTCAGGGGTGCGATGTGGGATTTGAATCCTTATTGCAAAGTTTGACTGAAGCAGAGAAACACTTTTTGCATAGCAAAAGGTTGCATATTGTTGGACTGCAATGGTTGGAAGACTGCTTGGAAAAGAACAAAAGACTGCATGAGGAATCATATAGTTTAAAACCCTATGGGTGGGAAGAATTCAACATCGAAGAGTGCAAACCTGACATGGTTTTAGAAGAAGCTCCCTGTGACAATGTGGAAATCCAGAAGGTTCCTTCTCTTTCTAACAAAGGGAAAAACAGAGGCAATGCAGCTCAAGAAAACTATGGGATCTTGGGCGCACCTGAGAGGGGTGGCAAAAGGAAAAGAGGAAGACCAGCTGGCACAAACACGAACAGAGGGAAAACAGGTGCCACGCAAGCTCGAAGAACACGTGCACGTATTGGCAGGAAGCCTGCTAAAATATGTGGCAATGAGTCAGATGAAAGTGGTTCTCATGATCGTACGTCATTTAAGGAAGAGGTTAATATCGCGGAAGGGAATCATGGGATGGTAGGTGACGGAAGTTCAGACATGCAAAGAAATGAGGCAATAAAAGATTCTACATCATTACAAAGTGGTAAGGCATCTGAACAAGAGGTTGCTGGAGACATTAGATTCGATGGCCATTCTGATAAAATTCCTGAAATTGAAATGATTGAGAAGCATAATGGCCAAGACAGAGCTAAGCCAGAGAAACTCGAATTTATGGCTGATCCAGTTCAAGCCATGTTGTTTGATCTGATTCCAAGTCTTGGAAAAAAGAATGCTCAAATTATGAATGTGAGTACTGAGGATGAGAAGCCACCCGTAGATCTGAATCCAAGTGCTGGAATGAAGAATGTTCAAACCACGCATACTAGTACTGAGGATGAGAAGCCACCGTTTGATATGGGTGCAGAGCCTACAAAGAAAGAGAAAGTTAGCTATAAGGATCTTGCTAGTCAGCTACTTAAGGATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1167

Amino Acids

131.69

Weight (kDa)

7.34

Isoelectric Point (pI)

42.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	5 - 170	2.8e-34	DNA ligase N terminus
DNA_ligase_A_M	PF01068	210 - 425	1.8e-42	ATP dependent DNA ligase domain
RNA_ligase	PF09414	229 - 424	2.6e-06	RNA ligase
DNA_ligase_A_C	PF04679	449 - 568	3.7e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	637 - 709	3.6e-07	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	639 - 720	3.7e-12	BRCT domain
PTCB-BRCT	PF12738	653 - 698	4.3e-09	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1316
AarI	CACCTGC	1 cut(s)	170
AatII	GACGTC	2 cut(s)	1164, 1345
Acc36I	ACCTGC	1 cut(s)	170
Acc65I	GGTACC	1 cut(s)	1763
AccB1I	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2909
AccB7I	CCANNNNNTGG	2 cut(s)	3293, 3368
AccII	CGCG	2 cut(s)	126, 3032
AccIII	TCCGGA	2 cut(s)	42, 511
AciI	CCGC	5 cut(s)	126, 234, 279, 629, 3032
AclI	AACGTT	1 cut(s)	534
AclWI	GGATC	6 cut(s)	449, 1227, 1818, 2837, 3248, 3480
AcoI	YGGCCR	2 cut(s)	3163, 3211
AcsI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2696, 3177, 3242
AcuI	CTGAAG	5 cut(s)	67, 540, 902, 1101, 2586
AcvI	CACGTG	1 cut(s)	2933
AcyI	GRCGYC	4 cut(s)	237, 249, 1161, 1342
AdeI	CACNNNGTG	1 cut(s)	2042
AfeI	AGCGCT	1 cut(s)	32
AfiI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2688, 2849, 3293, 3368
AflII	CTTAAG	2 cut(s)	140, 3491
AflIII	ACRYGT	1 cut(s)	2930
AhlI	ACTAGT	2 cut(s)	998, 3398
Alw21I	GWGCWC	2 cut(s)	156, 2938
Alw26I	GTCTC	4 cut(s)	186, 1558, 2352, 3141
Alw44I	GTGCAC	1 cut(s)	2934
AlwI	GGATC	6 cut(s)	449, 1227, 1818, 2837, 3248, 3480
Ama87I	CYCGRG	1 cut(s)	189
Aor13HI	TCCGGA	2 cut(s)	42, 511
Aor51HI	AGCGCT	1 cut(s)	32
AoxI	GGCC	6 cut(s)	136, 831, 1096, 1792, 3163, 3211
ApaLI	GTGCAC	1 cut(s)	2934
ApoI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2696, 3177, 3242
ArsI	GACNNNNNNTTYG	4 cut(s)	1557, 1589, 3140, 3172
AsiSI	GCGATCGC	1 cut(s)	721
Asp718I	GGTACC	1 cut(s)	1763
AspLEI	GCGC	8 cut(s)	33, 89, 126, 228, 239, 251, 627, 2840
AspS9I	GGNCC	2 cut(s)	1134, 2239
AsuHPI	GGTGA	5 cut(s)	380, 662, 866, 1100, 3068
AvaI	CYCGRG	1 cut(s)	189
AvaII	GGWCC	2 cut(s)	1134, 2239
BaeGI	GKGCMC	1 cut(s)	2938
BaeI	ACNNNNGTAYC	6 cut(s)	987, 1020, 1318, 1351, 2498, 2531
BalI	TGGCCA	2 cut(s)	3165, 3213
BanI	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2909
BarI	GAAGNNNNNNTAC	6 cut(s)	1265, 1297, 1493, 1525, 2156, 2188
BbrPI	CACGTG	1 cut(s)	2933
BbsI	GAAGAC	5 cut(s)	392, 422, 528, 2634, 2878
Bbv12I	GWGCWC	2 cut(s)	156, 2938
BbvCI	CCTCAGC	1 cut(s)	569
BccI	CCATC	5 cut(s)	845, 929, 1193, 3043, 3155
BceAI	ACGGC	3 cut(s)	311, 730, 2374
BciVI	GTATCC	3 cut(s)	1319, 1432, 2287
BclI	TGATCA	2 cut(s)	450, 778
BcoDI	GTCTC	4 cut(s)	186, 1558, 2352, 3141
BcuI	ACTAGT	2 cut(s)	998, 3398
BfaI	CTAG	5 cut(s)	999, 1242, 2283, 3399, 3480
BfmI	CTRYAG	1 cut(s)	1796
BfoI	RGCGCY	3 cut(s)	34, 240, 252
BfrI	CTTAAG	2 cut(s)	140, 3491
BfuAI	ACCTGC	1 cut(s)	170
BfuI	GTATCC	3 cut(s)	1319, 1432, 2287
BglII	AGATCT	2 cut(s)	463, 3352
BlpI	GCTNAGC	1 cut(s)	3225
BmcAI	AGTACT	3 cut(s)	2165, 3328, 3403
Bme18I	GGWCC	2 cut(s)	1134, 2239
BmeT110I	CYCGRG	1 cut(s)	189
BmgT120I	GGNCC	2 cut(s)	1134, 2239
BmsI	GCATC	8 cut(s)	371, 501, 505, 643, 732, 1456, 2482, 3134
BoxI	GACNNNNGTC	1 cut(s)	579
BpiI	GAAGAC	5 cut(s)	392, 422, 528, 2634, 2878
BpmI	CTGGAG	2 cut(s)	1025, 3165
Bpu10I	CCTNAGC	1 cut(s)	569
Bpu1102I	GCTNAGC	1 cut(s)	3225
BpuEI	CTTGAG	2 cut(s)	748, 2799
BsaAI	YACGTR	3 cut(s)	1679, 2933, 2939
BsaBI	GATNNNNATC	2 cut(s)	2542, 3357
BsaHI	GRCGYC	4 cut(s)	237, 249, 1161, 1342
BsaI	GGTCTC	2 cut(s)	1558, 2352
BsaJI	CCNNGG	2 cut(s)	282, 1706
BsaWI	WCCGGW	4 cut(s)	42, 365, 511, 2401
BsaXI	ACNNNNNCTCC	6 cut(s)	1341, 1371, 2330, 2360, 2415, 2445
Bsc4I	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2688, 2849, 3293, 3368
Bse118I	RCCGGY	2 cut(s)	245, 257
Bse1I	ACTGG	4 cut(s)	664, 1391, 1447, 3257
Bse3DI	GCAATG	5 cut(s)	1765, 2281, 2624, 2812, 2977
Bse8I	GATNNNNATC	2 cut(s)	2542, 3357
BseAI	TCCGGA	2 cut(s)	42, 511
BseDI	CCNNGG	2 cut(s)	282, 1706
BseJI	GATNNNNATC	2 cut(s)	2542, 3357
BseLI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2688, 2849, 3293, 3368
BseMI	GCAATG	5 cut(s)	1765, 2281, 2624, 2812, 2977
BseNI	ACTGG	4 cut(s)	664, 1391, 1447, 3257
BseRI	GAGGAG	1 cut(s)	1472
BseSI	GKGCMC	1 cut(s)	2938
BsgI	GTGCAG	2 cut(s)	1638, 3456
Bsh1236I	CGCG	2 cut(s)	126, 3032
Bsh1285I	CGRYCG	2 cut(s)	118, 721
BshFI	GGCC	6 cut(s)	138, 833, 1098, 1794, 3165, 3213
BshNI	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2909
BsiEI	CGRYCG	2 cut(s)	118, 721
BsiHKAI	GWGCWC	2 cut(s)	156, 2938
BsiHKCI	CYCGRG	1 cut(s)	189
BsiSI	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2402
BsiWI	CGTACG	1 cut(s)	3002
BslFI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2252
BslI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2688, 2849, 3293, 3368
BsmAI	GTCTC	4 cut(s)	186, 1558, 2352, 3141
BsmFI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2252
BsmI	GAATGC	3 cut(s)	971, 1721, 3310
BsnI	GGCC	6 cut(s)	138, 833, 1098, 1794, 3165, 3213
Bso31I	GGTCTC	2 cut(s)	1558, 2352
BsoBI	CYCGRG	1 cut(s)	189
Bsp1286I	GDGCHC	2 cut(s)	156, 2938
Bsp13I	TCCGGA	2 cut(s)	42, 511
Bsp1720I	GCTNAGC	1 cut(s)	3225
BspACI	CCGC	5 cut(s)	126, 234, 279, 629, 3032
BspANI	GGCC	6 cut(s)	138, 833, 1098, 1794, 3165, 3213
BspEI	TCCGGA	2 cut(s)	42, 511
BspFNI	CGCG	2 cut(s)	126, 3032
BspHI	TCATGA	3 cut(s)	447, 1009, 2995
BspMAI	CTGCAG	1 cut(s)	1800
BspMI	ACCTGC	1 cut(s)	170
BspPI	GGATC	6 cut(s)	449, 1227, 1818, 2837, 3248, 3480
BspQI	GCTCTTC	1 cut(s)	213
BspT107I	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2909
BspTI	CTTAAG	2 cut(s)	140, 3491
BspTNI	GGTCTC	2 cut(s)	1558, 2352
BsrDI	GCAATG	5 cut(s)	1765, 2281, 2624, 2812, 2977
BsrFI	RCCGGY	2 cut(s)	245, 257
BsrI	ACTGG	4 cut(s)	664, 1391, 1447, 3257
BssAI	RCCGGY	2 cut(s)	245, 257
BssECI	CCNNGG	2 cut(s)	282, 1706
BssNI	GRCGYC	4 cut(s)	237, 249, 1161, 1342
BssT1I	CCWWGG	1 cut(s)	1706
Bst4CI	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2330, 2431, 3423
Bst6I	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2703, 3012
BstACI	GRCGYC	4 cut(s)	237, 249, 1161, 1342
BstAFI	CTTAAG	2 cut(s)	140, 3491
BstAPI	GCANNNNNTGC	1 cut(s)	662
BstBAI	YACGTR	3 cut(s)	1679, 2933, 2939
BstC8I	GCNNGC	7 cut(s)	156, 259, 629, 1796, 2881, 2919, 2955
BstFNI	CGCG	2 cut(s)	126, 3032
BstH2I	RGCGCY	3 cut(s)	34, 240, 252
BstHHI	GCGC	8 cut(s)	33, 89, 126, 228, 239, 251, 627, 2840
BstMAI	GTCTC	4 cut(s)	186, 1558, 2352, 3141
BstMCI	CGRYCG	2 cut(s)	118, 721
BstMWI	GCNNNNNNNGC	7 cut(s)	234, 275, 295, 631, 662, 926, 2918
BstNSI	RCATGY	3 cut(s)	187, 800, 3076
BstPAI	GACNNNNGTC	1 cut(s)	579
BstSFI	CTRYAG	1 cut(s)	1796
BstSLI	GKGCMC	1 cut(s)	2938
BstUI	CGCG	2 cut(s)	126, 3032
BstV2I	GAAGAC	5 cut(s)	392, 422, 528, 2634, 2878
BstX2I	RGATCY	6 cut(s)	463, 1219, 1823, 2829, 3352, 3472
BstXI	CCANNNNNNTGG	2 cut(s)	1575, 2503
BstYI	RGATCY	6 cut(s)	463, 1219, 1823, 2829, 3352, 3472
BsuI	GTATCC	3 cut(s)	1319, 1432, 2287
BsuRI	GGCC	6 cut(s)	138, 833, 1098, 1794, 3165, 3213
BtgZI	GCGATG	2 cut(s)	815, 2544
BtsIMutI	CAGTG	3 cut(s)	952, 2038, 2482
BveI	ACCTGC	1 cut(s)	170
Cac8I	GCNNGC	7 cut(s)	156, 259, 629, 1796, 2881, 2919, 2955
CciI	TCATGA	3 cut(s)	447, 1009, 2995
CfoI	GCGC	8 cut(s)	33, 89, 126, 228, 239, 251, 627, 2840
Cfr10I	RCCGGY	2 cut(s)	245, 257
Cfr13I	GGNCC	2 cut(s)	1134, 2239
CseI	GACGC	2 cut(s)	182, 212
DinI	GGCGCC	2 cut(s)	238, 250
DraI	TTTAAA	4 cut(s)	1177, 1215, 1831, 2676
DraIII	CACNNNGTG	1 cut(s)	2042
EaeI	YGGCCR	2 cut(s)	3163, 3211
Eam1104I	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2703, 3012
EarI	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2703, 3012
Eco130I	CCWWGG	1 cut(s)	1706
Eco31I	GGTCTC	2 cut(s)	1558, 2352
Eco32I	GATATC	1 cut(s)	979
Eco47I	GGWCC	2 cut(s)	1134, 2239
Eco47III	AGCGCT	1 cut(s)	32
Eco57I	CTGAAG	5 cut(s)	67, 540, 902, 1101, 2586
Eco72I	CACGTG	1 cut(s)	2933
Eco88I	CYCGRG	1 cut(s)	189
EcoO109I	RGGNCCY	1 cut(s)	2239
EcoRI	GAATTC	1 cut(s)	2696
EcoRV	GATATC	1 cut(s)	979
EcoT14I	CCWWGG	1 cut(s)	1706
EgeI	GGCGCC	2 cut(s)	238, 250
EheI	GGCGCC	2 cut(s)	238, 250
ErhI	CCWWGG	1 cut(s)	1706
FalI	AAGNNNNNCTT	4 cut(s)	479, 511, 1811, 1843
FaqI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2252
FauI	CCCGC	1 cut(s)	636
FbaI	TGATCA	2 cut(s)	450, 778
FspBI	CTAG	5 cut(s)	999, 1242, 2283, 3399, 3480
GlaI	GCGC	8 cut(s)	32, 88, 125, 227, 238, 250, 626, 2839
GsuI	CTGGAG	2 cut(s)	1025, 3165
HaeII	RGCGCY	3 cut(s)	34, 240, 252
HaeIII	GGCC	6 cut(s)	138, 833, 1098, 1794, 3165, 3213
HapII	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2402
HgaI	GACGC	2 cut(s)	182, 212
HhaI	GCGC	8 cut(s)	33, 89, 126, 228, 239, 251, 627, 2840
Hin1I	GRCGYC	4 cut(s)	237, 249, 1161, 1342
Hin6I	GCGC	8 cut(s)	31, 87, 124, 226, 237, 249, 625, 2838
HinP1I	GCGC	8 cut(s)	31, 87, 124, 226, 237, 249, 625, 2838
HincII	GTYRAC	1 cut(s)	584
HindII	GTYRAC	1 cut(s)	584
HpaII	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2402
HphI	GGTGA	5 cut(s)	380, 662, 866, 1100, 3068
Hpy166II	GTNNAC	3 cut(s)	320, 584, 2936
Hpy8I	GTNNAC	3 cut(s)	320, 584, 2936
Hpy99I	CGWCG	7 cut(s)	176, 203, 206, 278, 716, 1166, 1347
HpyAV	CCTTC	7 cut(s)	1015, 1407, 2156, 2430, 2761, 2784, 3029
HpyCH4III	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2330, 2431, 3423
HpyF10VI	GCNNNNNNNGC	7 cut(s)	234, 275, 295, 631, 662, 926, 2918
Hsp92I	GRCGYC	4 cut(s)	237, 249, 1161, 1342
HspAI	GCGC	8 cut(s)	31, 87, 124, 226, 237, 249, 625, 2838
KasI	GGCGCC	2 cut(s)	236, 248
Kpn2I	TCCGGA	2 cut(s)	42, 511
KpnI	GGTACC	1 cut(s)	1767
KroI	GCCGGC	1 cut(s)	257
KroNI	GCCGGC	1 cut(s)	259
Ksp22I	TGATCA	2 cut(s)	450, 778
LguI	GCTCTTC	1 cut(s)	213
LmnI	GCTCC	3 cut(s)	38, 121, 2746
LweI	GCATC	8 cut(s)	371, 501, 505, 643, 732, 1456, 2482, 3134
MaeI	CTAG	5 cut(s)	999, 1242, 2283, 3399, 3480
MaeIII	GTNAC	8 cut(s)	535, 542, 1619, 2032, 2425, 2477, 2747, 3056
MfeI	CAATTG	1 cut(s)	220
MflI	RGATCY	6 cut(s)	463, 1219, 1823, 2829, 3352, 3472
MhlI	GDGCHC	2 cut(s)	156, 2938
MlsI	TGGCCA	2 cut(s)	3165, 3213
MluNI	TGGCCA	2 cut(s)	3165, 3213
Mly113I	GGCGCC	2 cut(s)	237, 249
MlyI	GAGTC	5 cut(s)	155, 188, 2177, 2481, 2984
MmeI	TCCRAC	6 cut(s)	222, 592, 970, 1979, 2590, 2604
Mox20I	TGGCCA	2 cut(s)	3165, 3213
MroI	TCCGGA	2 cut(s)	42, 511
MroNI	GCCGGC	1 cut(s)	257
MscI	TGGCCA	2 cut(s)	3165, 3213
MslI	CAYNNNNRTG	2 cut(s)	513, 738
Msp20I	TGGCCA	2 cut(s)	3165, 3213
MspA1I	CMGCKG	2 cut(s)	2051, 2879
MspCI	CTTAAG	2 cut(s)	140, 3491
MspI	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2402
MunI	CAATTG	1 cut(s)	220
Mva1269I	GAATGC	3 cut(s)	971, 1721, 3310
MvnI	CGCG	2 cut(s)	126, 3032
MwoI	GCNNNNNNNGC	7 cut(s)	234, 275, 295, 631, 662, 926, 2918
NaeI	GCCGGC	1 cut(s)	259
NarI	GGCGCC	2 cut(s)	237, 249
NgoMIV	GCCGGC	1 cut(s)	257
NmeAIII	GCCGAG	2 cut(s)	159, 307
NmuCI	GTSAC	6 cut(s)	542, 1619, 2032, 2477, 2747, 3056
NspI	RCATGY	3 cut(s)	187, 800, 3076
PagI	TCATGA	3 cut(s)	447, 1009, 2995
PaqCI	CACCTGC	1 cut(s)	170
PciSI	GCTCTTC	1 cut(s)	213
PcsI	WCGNNNNNNNCGW	4 cut(s)	279, 592, 1684, 2929
PctI	GAATGC	3 cut(s)	971, 1721, 3310
PdiI	GCCGGC	1 cut(s)	259
Pfl23II	CGTACG	1 cut(s)	3002
PflMI	CCANNNNNTGG	2 cut(s)	3293, 3368
Ple19I	CGATCG	2 cut(s)	118, 721
PleI	GAGTC	5 cut(s)	154, 188, 2177, 2480, 2983
PluTI	GGCGCC	2 cut(s)	240, 252
PmaCI	CACGTG	1 cut(s)	2933
PmlI	CACGTG	1 cut(s)	2933
PpsI	GAGTC	5 cut(s)	154, 188, 2177, 2480, 2983
Ppu21I	YACGTR	3 cut(s)	1679, 2933, 2939
PpuMI	RGGWCCY	1 cut(s)	2239
PshAI	GACNNNNGTC	1 cut(s)	579
PsiI	TTATAA	1 cut(s)	1316
Psp1406I	AACGTT	1 cut(s)	534
Psp5II	RGGWCCY	1 cut(s)	2239
PspCI	CACGTG	1 cut(s)	2933
PspLI	CGTACG	1 cut(s)	3002
PspPI	GGNCC	2 cut(s)	1134, 2239
PspPPI	RGGWCCY	1 cut(s)	2239
PstI	CTGCAG	1 cut(s)	1800
PsuI	RGATCY	6 cut(s)	463, 1219, 1823, 2829, 3352, 3472
PvuI	CGATCG	2 cut(s)	118, 721
PvuII	CAGCTG	2 cut(s)	2051, 2879
RgaI	GCGATCGC	1 cut(s)	721
RseI	CAYNNNNRTG	2 cut(s)	513, 738
SapI	GCTCTTC	1 cut(s)	213
Sau96I	GGNCC	2 cut(s)	1134, 2239
SbfI	CCTGCAGG	1 cut(s)	1800
ScaI	AGTACT	3 cut(s)	2165, 3328, 3403
SchI	GAGTC	5 cut(s)	155, 188, 2177, 2481, 2984
SdaI	CCTGCAGG	1 cut(s)	1800
SduI	GDGCHC	2 cut(s)	156, 2938
SfaAI	GCGATCGC	1 cut(s)	721
SfaNI	GCATC	8 cut(s)	371, 501, 505, 643, 732, 1456, 2482, 3134
SfcI	CTRYAG	1 cut(s)	1796
SfoI	GGCGCC	2 cut(s)	238, 250
SgfI	GCGATCGC	1 cut(s)	721
SinI	GGWCC	2 cut(s)	1134, 2239
SmiMI	CAYNNNNRTG	2 cut(s)	513, 738
SmlI	CTYRAG	4 cut(s)	140, 763, 2814, 3491
SmoI	CTYRAG	4 cut(s)	140, 763, 2814, 3491
SpeI	ACTAGT	2 cut(s)	998, 3398
Sse8387I	CCTGCAGG	1 cut(s)	1800
SsiI	CCGC	5 cut(s)	126, 234, 279, 629, 3032
SspDI	GGCGCC	2 cut(s)	236, 248
SspI	AATATT	1 cut(s)	1917
SspMI	CTAG	5 cut(s)	999, 1242, 2283, 3399, 3480
StyI	CCWWGG	1 cut(s)	1706
TaaI	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2330, 2431, 3423
TatI	WGTACW	4 cut(s)	751, 2163, 3326, 3401
TauI	GCSGC	2 cut(s)	237, 281
TscAI	CASTG	3 cut(s)	952, 2045, 2482
TseFI	GTSAC	6 cut(s)	542, 1619, 2032, 2477, 2747, 3056
Tsp45I	GTSAC	6 cut(s)	542, 1619, 2032, 2477, 2747, 3056
TspGWI	ACGGA	1 cut(s)	3075
TspRI	CASTG	3 cut(s)	952, 2045, 2482
Van91I	CCANNNNNTGG	2 cut(s)	3293, 3368
Vha464I	CTTAAG	2 cut(s)	140, 3491
VneI	GTGCAC	1 cut(s)	2934
VpaK11BI	GGWCC	2 cut(s)	1134, 2239
XapI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2696, 3177, 3242
XceI	RCATGY	3 cut(s)	187, 800, 3076
XspI	CTAG	5 cut(s)	999, 1242, 2283, 3399, 3480
ZraI	GACGTC	2 cut(s)	1162, 1343
ZrmI	AGTACT	3 cut(s)	2165, 3328, 3403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.