Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

16474434 .. 16486863

Gene Structure 12430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ37144

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3549 bp

ATGAATAAGGAGGAGACCAAGTTCAGTGTGCTGTGCAGCCTCTTCAATTGGATGCAACGAAGCAAAACGTCAGCCCTAAAGCGCTCCAAGTTCCGGAAATTCCTTGACAACTTCTGCAAAGCAGGGGATTACTTCAGCGCAATCCGGCTGATTCTTCCGAGCCTCGATCGGGAGCGCGGTAGCTATGGCCTTAAGGAGTCGGTGCTCGCCACCTGCCTCATCGACGCTCTCGGCATGTCCCGAGACTCCGACGACGCTCTTCGCCTCATCAATTGGCGCAAGGGCGGCGCCAAGACCGGTGCCAATGCCGGCAATTTCGCCCTCGTCGCCGCCGAGGTGCTGCAACGGAGACAGGGACTTAGTTCAGGTGGACTAACGATTAAGGAGTTGAATGACTTGCTTGATCGTTTGGCATCCGGTGAAAATAGGGCAGAGAAGACTTCAATTCTTTCTACTTTGGTACAGAAGACAAATGCACAAGAAATGAAGTGGATCGTCATGATCATTCTTAAAGATCTCAAATTGGGAATTAGTGAAAAGAGCATCTTCCATGAATTTCATCCGGATGCTGAAGACTTGTTTAACGTTACCTGTGACTTGAAATTGGTTTGTGAAAAGCTGAGGGACAGAAGTCAACGACATAAACGACAGGACATTGAAGTTGGAAAAGCTGTGCGCCCGCAGCTAGCTATGAGAGTTGGTGATGCAACTGGTGCATGGAAAAAGTTTCATGGAAAGGAAGTTGTTGTTGAGTGTAAATTCGACGGCGATCGCATCCAAATTCATAAGAATGGAACCGAAGTACATTACTTCTCAAGGAATTTTCTTGATCACTCTGAATATGGACATGCAATGTCAGACATTATTATACAAAATGTTCAGGCTGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTCTGAAGCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCAAAGGCAGCGAGGGATGGACTTGACAGTGATAAACAGTTGTGCTATGTTGCATTCGATATCCTTTACGTTGGAGATACTAGTGTAATTCACGAGAGCTTGAAGGGAAGGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATTTTAGTACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGGGGAACCTTGTTGGTCCCTAATTGTTCATACTCTGGATGATGTCGAGAGGTTTTTTAAAGAAACCATCGAGAACAGAGATGAGGGGATTGTTTTAAAGGATCTTGGTTCTAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTGAAATTGAAGCCGGATTACATACGGGCTGGTTCTGATCTGGATGTTCTTATAATAGGAGGATACTATGGTTCAGGACGTCGTGGAGGGGAAGTAGCTCAGTTCTTGGTGGGTCTTGCTGAACGTCCAGTTGCAAATACATATCCGAGAAGGTTTGTATCCTTTTGCAGAGTGGGTACTGGACTTTCTGACGAGGAGTTAGATGCTGTGGCAACCAAATTAAAGCCTTATTTAAGGAAGTATGACTACCCCAAAAAGGCACCACCAAGTTTTTATGAAGTAACGAACCATTCAAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATCCTTTCCATAACAAGTGACATCCGAACCATAAGGTCCGAGGTTTTTGCTGCACCATACAGCCTGAGATTTCCACGTATTGATCGAATTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAATCATTTATAGAGCTTGTAGATTCAGGCAATGGTACCACACAAAAGGGGACAGATTATGGTGGCCAGCAGGATGATAAAAAGAAACACAAGAGATCCTCTAAAAAGGAAGAGAAAAGAAATCTATCTCTTGTTCCCTCTCATTTAGTTCAGACTGATGTTTCTGGTGTCAAGGAGGACAGCTTAATATTTTCAAAAATGATGTTCTACTTTGCTAACGTGCCTCCAACTCATTCTCTTGATTCCTTGCACAAAATTGTTGCTGAGAATGGGGGTACTTTTTCGATGAATTTGAATAACTCAGTCACACACTGTGTTGCAGCTGATAGCAAAGGGATTAAATATCAGGCAGCAAAACGTCAGGGAGATGTGATTCATTTGTCTTGGGTCTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCTTTACAGCCAAAGTACTACCTCTTCATTTCTGACTCTTCAAAGAAGAAGTTAGAAGAAGAAATAGATGAGTTTTCTGACCCGTACTTCTGGGACCTTGACCTTGCAGACATTAAGCAGCTTCTAAGCAATGTGCCTAGATCAGAGGATACCAAACGTATCAACTATTACAAGAAAAAATACTGTCCTATGGAGAAATGGTCTCGATTTCACGGCTGCTGCATTTACTTTAACTCATCAATTCATTCCTTGAAACCGGATTGGGAAGTTGTGTTAGGAGTTACAGTAAGAAGGTTAAAGATTGAAGTTCTCATGGGTGGTGGCAGAGTCTGTGACAATCTTGCTGATGCCACCCATTTGGTACTCTTGTCAATTCAGGGGTGCGATGTCGGATTCGAATCCTTATTGCAAAGTTTGACTGAAGCAGAGAAACACTTTTTGCATAGCAAAAGGTTGCATATTGTTGGACTGCAATGGTTGGAAGACAGCTTGGAAAAGAACAAAAGACTGCATGAGGAATCATATAGTTTAAAACCCTATGGGTGGGAAGAATTCAACATCGAAGAGTGCAAACCTGACATGGTTTTAGAAGAAGCTCCCTGTGACAATGTGGAAATCCAGAAGGTTCCTTCTCTTTCTAACAAATGGAAAAACAGAGGCAATGCAGCTCAAGAAAACTATGGGATCTTGGGCGCACCTGGCAAAAGGAAAAGAGGAAGACCAGCTGGCACAAACACGAACAGAGGGAAAACAGGTGCCACGCAAGCTCGAAGAACACGTGCACGTATTGGCAGGAAGCCTGCTAAAATATCTGGCAATGAGTCAGATGAAAGTGGTTCTCATGATCGTACGTCATTTAAGGAAGAGATTAATATCGCGGAAGGGAATCATGGGATGGTAGGTGACGGAGGTTCAGACATGCAAAGAAATGAGGCAATAAAAGATTCTACATCATTACAAAGTGGTAAGGCAGCTGAACAAGAGGTTGCTGGAGACATTAGATTCGATGGCCATTCTGATAAAATTCCTGAAATTGAAATGATTGAGAAGCATAATGGCCAAGACAGAGCTAAGCCAGAGAAACTCGAATTTATGGCTGATCCAGTTCAAGCCATGTTGTTTGATCTGATTCCAAGTCTTGGAAAAAAGAATGTTCAAACTATGAATGTGAGTACTGAGGATGAGAAGCCACCCGTAGATCTGAATCCAAGTCCTGGAATGAAGAATGTTCAAACCACGCATACTAGTACTGAGGATGAGAAGCCACCGTTTGATATGGGTGCAGAGCCTCCAAAGAAAAAGAAAGTTAGCTATAAGGATCTTGCTAGTCAGCTACTTAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1182

Amino Acids

133.52

Weight (kDa)

8.13

Isoelectric Point (pI)

43.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	7 - 187	5.3e-38	DNA ligase N terminus
DNA_ligase_A_M	PF01068	227 - 442	2.2e-42	ATP dependent DNA ligase domain
DNA_ligase_A_C	PF04679	466 - 585	2.5e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	654 - 726	1.1e-06	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	656 - 737	3.3e-12	BRCT domain
PTCB-BRCT	PF12738	671 - 715	8.1e-09	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1367
AarI	CACCTGC	1 cut(s)	221
AatII	GACGTC	1 cut(s)	1396
Acc36I	ACCTGC	1 cut(s)	221
Acc65I	GGTACC	1 cut(s)	1814
AccB1I	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2954
AccB7I	CCANNNNNTGG	2 cut(s)	3338, 3413
AccII	CGCG	2 cut(s)	177, 3077
AccIII	TCCGGA	2 cut(s)	93, 562
AciI	CCGC	5 cut(s)	177, 285, 330, 680, 3077
AclI	AACGTT	1 cut(s)	585
AclWI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3293, 3525
AcoI	YGGCCR	3 cut(s)	1843, 3208, 3256
AcsI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3222, 3287
AcuI	CTGAAG	5 cut(s)	118, 591, 953, 1152, 2640
AcvI	CACGTG	1 cut(s)	2978
AcyI	GRCGYC	2 cut(s)	288, 1393
AdeI	CACNNNGTG	1 cut(s)	2093
AfeI	AGCGCT	1 cut(s)	83
AfiI	CCNNNNNNNGG	8 cut(s)	93, 169, 1115, 1145, 1570, 2742, 3338, 3413
AflII	CTTAAG	2 cut(s)	191, 3536
AflIII	ACRYGT	1 cut(s)	2975
AgeI	ACCGGT	1 cut(s)	296
AhlI	ACTAGT	2 cut(s)	1049, 3443
AjnI	CCWGG	2 cut(s)	2896, 3412
AloI	GAACNNNNNNTCC	1 cut(s)	37
Alw21I	GWGCWC	2 cut(s)	207, 2983
Alw26I	GTCTC	6 cut(s)	8, 237, 343, 1609, 2406, 3186
Alw44I	GTGCAC	1 cut(s)	2979
AlwI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3293, 3525
AlwNI	CAGNNNCTG	1 cut(s)	2529
Ama87I	CYCGRG	1 cut(s)	240
Aor13HI	TCCGGA	2 cut(s)	93, 562
Aor51HI	AGCGCT	1 cut(s)	83
AoxI	GGCC	5 cut(s)	187, 1147, 1843, 3208, 3256
ApaLI	GTGCAC	1 cut(s)	2979
ApoI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3222, 3287
ArsI	GACNNNNNNTTYG	4 cut(s)	1608, 1640, 3185, 3217
AseI	ATTAAT	1 cut(s)	3069
AsiGI	ACCGGT	1 cut(s)	296
AsiSI	GCGATCGC	1 cut(s)	772
Asp718I	GGTACC	1 cut(s)	1814
AspLEI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
AspS9I	GGNCC	3 cut(s)	1185, 1689, 2293
AsuHPI	GGTGA	5 cut(s)	431, 713, 917, 1151, 3113
AsuII	TTCGAA	1 cut(s)	2595
AsuNHI	GCTAGC	1 cut(s)	685
AvaI	CYCGRG	1 cut(s)	240
AvaII	GGWCC	3 cut(s)	1185, 1689, 2293
BaeGI	GKGCMC	1 cut(s)	2983
BaeI	ACNNNNGTAYC	6 cut(s)	1038, 1071, 1369, 1402, 2552, 2585
BalI	TGGCCA	3 cut(s)	1845, 3210, 3258
BanI	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2954
BarI	GAAGNNNNNNTAC	6 cut(s)	1316, 1348, 1544, 1576, 2207, 2239
BauI	CACGAG	1 cut(s)	1061
BbrPI	CACGTG	1 cut(s)	2978
BbsI	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2923
Bbv12I	GWGCWC	2 cut(s)	207, 2983
BbvCI	CCTCAGC	1 cut(s)	620
BccI	CCATC	5 cut(s)	896, 980, 1244, 3088, 3200
BceAI	ACGGC	2 cut(s)	781, 2428
BcgI	CGANNNNNNTGC	2 cut(s)	1682, 1716
BciT130I	CCWGG	2 cut(s)	2898, 3414
BciVI	GTATCC	3 cut(s)	1370, 1483, 2341
BclI	TGATCA	2 cut(s)	501, 829
BcoDI	GTCTC	6 cut(s)	8, 237, 343, 1609, 2406, 3186
BcuI	ACTAGT	2 cut(s)	1049, 3443
BfaI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3444, 3525
BfoI	RGCGCY	2 cut(s)	85, 291
BfrI	CTTAAG	2 cut(s)	191, 3536
BfuAI	ACCTGC	1 cut(s)	221
BfuI	GTATCC	3 cut(s)	1370, 1483, 2341
BglII	AGATCT	2 cut(s)	514, 3397
BlpI	GCTNAGC	1 cut(s)	3270
BmcAI	AGTACT	3 cut(s)	2216, 3373, 3448
Bme1390I	CCNGG	2 cut(s)	2898, 3414
Bme18I	GGWCC	3 cut(s)	1185, 1689, 2293
BmeT110I	CYCGRG	1 cut(s)	240
BmgT120I	GGNCC	3 cut(s)	1185, 1689, 2293
BmrFI	CCNGG	2 cut(s)	2898, 3414
BmsI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
BmtI	GCTAGC	1 cut(s)	689
BoxI	GACNNNNGTC	1 cut(s)	630
BpiI	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2923
BpmI	CTGGAG	2 cut(s)	1076, 3210
Bpu10I	CCTNAGC	1 cut(s)	620
Bpu1102I	GCTNAGC	1 cut(s)	3270
Bpu14I	TTCGAA	1 cut(s)	2595
BpuEI	CTTGAG	2 cut(s)	799, 2853
BsaAI	YACGTR	3 cut(s)	1730, 2978, 2984
BsaBI	GATNNNNATC	3 cut(s)	2596, 3071, 3402
BsaHI	GRCGYC	2 cut(s)	288, 1393
BsaI	GGTCTC	3 cut(s)	8, 1609, 2406
BsaJI	CCNNGG	3 cut(s)	333, 1692, 1757
BsaWI	WCCGGW	5 cut(s)	93, 296, 416, 562, 2455
BsaXI	ACNNNNNCTCC	8 cut(s)	5, 35, 1392, 1422, 2384, 2414, 2469, 2499
Bsc4I	CCNNNNNNNGG	8 cut(s)	93, 169, 1115, 1145, 1570, 2742, 3338, 3413
Bse118I	RCCGGY	2 cut(s)	296, 308
Bse1I	ACTGG	4 cut(s)	715, 1442, 1498, 3302
Bse3DI	GCAATG	6 cut(s)	858, 1816, 2335, 2678, 2866, 3022
Bse8I	GATNNNNATC	3 cut(s)	2596, 3071, 3402
BseAI	TCCGGA	2 cut(s)	93, 562
BseBI	CCWGG	2 cut(s)	2898, 3414
BseDI	CCNNGG	3 cut(s)	333, 1692, 1757
BseJI	GATNNNNATC	3 cut(s)	2596, 3071, 3402
BseLI	CCNNNNNNNGG	8 cut(s)	93, 169, 1115, 1145, 1570, 2742, 3338, 3413
BseMI	GCAATG	6 cut(s)	858, 1816, 2335, 2678, 2866, 3022
BseMII	CTCAG	9 cut(s)	611, 936, 1427, 1632, 1709, 2034, 2094, 3366, 3441
BseNI	ACTGG	4 cut(s)	715, 1442, 1498, 3302
BseRI	GAGGAG	2 cut(s)	26, 1523
BseSI	GKGCMC	1 cut(s)	2983
BsgI	GTGCAG	3 cut(s)	55, 1689, 3501
Bsh1236I	CGCG	2 cut(s)	177, 3077
Bsh1285I	CGRYCG	2 cut(s)	169, 772
BshFI	GGCC	5 cut(s)	189, 1149, 1845, 3210, 3258
BshNI	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2954
BshTI	ACCGGT	1 cut(s)	296
BsiEI	CGRYCG	2 cut(s)	169, 772
BsiHKAI	GWGCWC	2 cut(s)	207, 2983
BsiHKCI	CYCGRG	1 cut(s)	240
BsiSI	CCGG	8 cut(s)	94, 145, 297, 309, 417, 563, 1328, 2456
BsiWI	CGTACG	1 cut(s)	3047
BslFI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
BslI	CCNNNNNNNGG	8 cut(s)	93, 169, 1115, 1145, 1570, 2742, 3338, 3413
BsmAI	GTCTC	6 cut(s)	8, 237, 343, 1609, 2406, 3186
BsmFI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
BsmI	GAATGC	2 cut(s)	1022, 1772
BsnI	GGCC	5 cut(s)	189, 1149, 1845, 3210, 3258
Bso31I	GGTCTC	3 cut(s)	8, 1609, 2406
BsoBI	CYCGRG	1 cut(s)	240
Bsp119I	TTCGAA	1 cut(s)	2595
Bsp1286I	GDGCHC	2 cut(s)	207, 2983
Bsp13I	TCCGGA	2 cut(s)	93, 562
Bsp1720I	GCTNAGC	1 cut(s)	3270
BspACI	CCGC	5 cut(s)	177, 285, 330, 680, 3077
BspANI	GGCC	5 cut(s)	189, 1149, 1845, 3210, 3258
BspCNI	CTCAG	9 cut(s)	612, 937, 1426, 1633, 1710, 2035, 2093, 3367, 3442
BspEI	TCCGGA	2 cut(s)	93, 562
BspFNI	CGCG	2 cut(s)	177, 3077
BspHI	TCATGA	2 cut(s)	498, 3040
BspMI	ACCTGC	1 cut(s)	221
BspOI	GCTAGC	1 cut(s)	689
BspPI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3293, 3525
BspQI	GCTCTTC	1 cut(s)	264
BspT104I	TTCGAA	1 cut(s)	2595
BspT107I	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2954
BspTI	CTTAAG	2 cut(s)	191, 3536
BspTNI	GGTCTC	3 cut(s)	8, 1609, 2406
BsrDI	GCAATG	6 cut(s)	858, 1816, 2335, 2678, 2866, 3022
BsrFI	RCCGGY	2 cut(s)	296, 308
BsrI	ACTGG	4 cut(s)	715, 1442, 1498, 3302
BssAI	RCCGGY	2 cut(s)	296, 308
BssECI	CCNNGG	3 cut(s)	333, 1692, 1757
BssNI	GRCGYC	2 cut(s)	288, 1393
BssSI	CACGAG	1 cut(s)	1061
BssT1I	CCWWGG	1 cut(s)	1757
Bst2BI	CACGAG	1 cut(s)	1061
Bst2UI	CCWGG	2 cut(s)	2898, 3414
Bst4CI	ACNGT	7 cut(s)	998, 1008, 1163, 2093, 2384, 2485, 3468
Bst6I	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3057
BstACI	GRCGYC	2 cut(s)	288, 1393
BstAFI	CTTAAG	2 cut(s)	191, 3536
BstAPI	GCANNNNNTGC	1 cut(s)	713
BstBAI	YACGTR	3 cut(s)	1730, 2978, 2984
BstBI	TTCGAA	1 cut(s)	2595
BstC8I	GCNNGC	8 cut(s)	207, 310, 680, 687, 1847, 2926, 2964, 3000
BstFNI	CGCG	2 cut(s)	177, 3077
BstH2I	RGCGCY	2 cut(s)	85, 291
BstHHI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
BstMAI	GTCTC	6 cut(s)	8, 237, 343, 1609, 2406, 3186
BstMCI	CGRYCG	2 cut(s)	169, 772
BstMWI	GCNNNNNNNGC	7 cut(s)	285, 326, 682, 713, 977, 2898, 2963
BstNI	CCWGG	2 cut(s)	2898, 3414
BstNSI	RCATGY	3 cut(s)	238, 851, 3121
BstPAI	GACNNNNGTC	1 cut(s)	630
BstSCI	CCNGG	2 cut(s)	2896, 3412
BstSLI	GKGCMC	1 cut(s)	2983
BstUI	CGCG	2 cut(s)	177, 3077
BstV2I	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2923
BstX2I	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3397, 3517
BstXI	CCANNNNNNTGG	2 cut(s)	1626, 2557
BstYI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3397, 3517
BsuI	GTATCC	3 cut(s)	1370, 1483, 2341
BsuRI	GGCC	5 cut(s)	189, 1149, 1845, 3210, 3258
BtgZI	GCGATG	1 cut(s)	2598
BtsIMutI	CAGTG	3 cut(s)	31, 1003, 2089
BveI	ACCTGC	1 cut(s)	221
Cac8I	GCNNGC	8 cut(s)	207, 310, 680, 687, 1847, 2926, 2964, 3000
CaiI	CAGNNNCTG	1 cut(s)	2529
CciI	TCATGA	2 cut(s)	498, 3040
CfoI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
Cfr10I	RCCGGY	2 cut(s)	296, 308
Cfr13I	GGNCC	3 cut(s)	1185, 1689, 2293
CseI	GACGC	2 cut(s)	233, 263
CspAI	ACCGGT	1 cut(s)	296
DinI	GGCGCC	1 cut(s)	289
DraI	TTTAAA	3 cut(s)	1228, 1266, 2730
DraIII	CACNNNGTG	1 cut(s)	2093
EaeI	YGGCCR	3 cut(s)	1843, 3208, 3256
Eam1104I	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3057
EarI	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3057
Eco130I	CCWWGG	1 cut(s)	1757
Eco31I	GGTCTC	3 cut(s)	8, 1609, 2406
Eco32I	GATATC	1 cut(s)	1030
Eco47I	GGWCC	3 cut(s)	1185, 1689, 2293
Eco47III	AGCGCT	1 cut(s)	83
Eco57I	CTGAAG	5 cut(s)	118, 591, 953, 1152, 2640
Eco72I	CACGTG	1 cut(s)	2978
Eco88I	CYCGRG	1 cut(s)	240
EcoO109I	RGGNCCY	1 cut(s)	2293
EcoRI	GAATTC	1 cut(s)	2750
EcoRII	CCWGG	2 cut(s)	2896, 3412
EcoRV	GATATC	1 cut(s)	1030
EcoT14I	CCWWGG	1 cut(s)	1757
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	1 cut(s)	1757
FalI	AAGNNNNNCTT	2 cut(s)	530, 562
FaqI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
FauI	CCCGC	1 cut(s)	687
FbaI	TGATCA	2 cut(s)	501, 829
FspBI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3444, 3525
GlaI	GCGC	7 cut(s)	83, 139, 176, 278, 289, 677, 2893
GsuI	CTGGAG	2 cut(s)	1076, 3210
HaeII	RGCGCY	2 cut(s)	85, 291
HaeIII	GGCC	5 cut(s)	189, 1149, 1845, 3210, 3258
HapII	CCGG	8 cut(s)	94, 145, 297, 309, 417, 563, 1328, 2456
HgaI	GACGC	2 cut(s)	233, 263
HhaI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
Hin1I	GRCGYC	2 cut(s)	288, 1393
Hin6I	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
HinP1I	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
HincII	GTYRAC	1 cut(s)	635
HindII	GTYRAC	1 cut(s)	635
HpaII	CCGG	8 cut(s)	94, 145, 297, 309, 417, 563, 1328, 2456
HphI	GGTGA	5 cut(s)	431, 713, 917, 1151, 3113
Hpy166II	GTNNAC	3 cut(s)	371, 635, 2981
Hpy8I	GTNNAC	3 cut(s)	371, 635, 2981
Hpy99I	CGWCG	6 cut(s)	227, 254, 257, 329, 767, 1398
HpyAV	CCTTC	8 cut(s)	1066, 1071, 1458, 2207, 2484, 2815, 2838, 3074
HpyCH4III	ACNGT	7 cut(s)	998, 1008, 1163, 2093, 2384, 2485, 3468
HpyF10VI	GCNNNNNNNGC	7 cut(s)	285, 326, 682, 713, 977, 2898, 2963
Hsp92I	GRCGYC	2 cut(s)	288, 1393
HspAI	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
KasI	GGCGCC	1 cut(s)	287
Kpn2I	TCCGGA	2 cut(s)	93, 562
KpnI	GGTACC	1 cut(s)	1818
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
Ksp22I	TGATCA	2 cut(s)	501, 829
LguI	GCTCTTC	1 cut(s)	264
LmnI	GCTCC	3 cut(s)	89, 172, 2800
LweI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
MaeI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3444, 3525
MaeIII	GTNAC	9 cut(s)	586, 593, 1594, 1670, 2083, 2479, 2531, 2801, 3101
MfeI	CAATTG	2 cut(s)	46, 271
MflI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3397, 3517
MhlI	GDGCHC	2 cut(s)	207, 2983
MlsI	TGGCCA	3 cut(s)	1845, 3210, 3258
MluNI	TGGCCA	3 cut(s)	1845, 3210, 3258
Mly113I	GGCGCC	1 cut(s)	288
MlyI	GAGTC	5 cut(s)	206, 239, 2228, 2535, 3029
MmeI	TCCRAC	7 cut(s)	273, 643, 1021, 2030, 2569, 2644, 2658
Mox20I	TGGCCA	3 cut(s)	1845, 3210, 3258
MroI	TCCGGA	2 cut(s)	93, 562
MroNI	GCCGGC	1 cut(s)	308
MscI	TGGCCA	3 cut(s)	1845, 3210, 3258
MslI	CAYNNNNRTG	2 cut(s)	564, 789
Msp20I	TGGCCA	3 cut(s)	1845, 3210, 3258
MspA1I	CMGCKG	3 cut(s)	2102, 2924, 3173
MspCI	CTTAAG	2 cut(s)	191, 3536
MspI	CCGG	8 cut(s)	94, 145, 297, 309, 417, 563, 1328, 2456
MspR9I	CCNGG	2 cut(s)	2898, 3414
MunI	CAATTG	2 cut(s)	46, 271
Mva1269I	GAATGC	2 cut(s)	1022, 1772
MvaI	CCWGG	2 cut(s)	2898, 3414
MvnI	CGCG	2 cut(s)	177, 3077
MwoI	GCNNNNNNNGC	7 cut(s)	285, 326, 682, 713, 977, 2898, 2963
NaeI	GCCGGC	1 cut(s)	310
NarI	GGCGCC	1 cut(s)	288
NgoMIV	GCCGGC	1 cut(s)	308
NheI	GCTAGC	1 cut(s)	685
NmeAIII	GCCGAG	2 cut(s)	210, 358
NmuCI	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3101
NspI	RCATGY	3 cut(s)	238, 851, 3121
NspV	TTCGAA	1 cut(s)	2595
PagI	TCATGA	2 cut(s)	498, 3040
PaqCI	CACCTGC	1 cut(s)	221
PciSI	GCTCTTC	1 cut(s)	264
PcsI	WCGNNNNNNNCGW	4 cut(s)	330, 643, 1735, 2974
PctI	GAATGC	2 cut(s)	1022, 1772
PdiI	GCCGGC	1 cut(s)	310
Pfl23II	CGTACG	1 cut(s)	3047
PflMI	CCANNNNNTGG	2 cut(s)	3338, 3413
PfoI	TCCNGGA	1 cut(s)	3412
PinAI	ACCGGT	1 cut(s)	296
Ple19I	CGATCG	2 cut(s)	169, 772
PleI	GAGTC	5 cut(s)	205, 239, 2228, 2534, 3028
PluTI	GGCGCC	1 cut(s)	291
PmaCI	CACGTG	1 cut(s)	2978
PmlI	CACGTG	1 cut(s)	2978
PpsI	GAGTC	5 cut(s)	205, 239, 2228, 2534, 3028
Ppu21I	YACGTR	3 cut(s)	1730, 2978, 2984
PpuMI	RGGWCCY	1 cut(s)	2293
PshAI	GACNNNNGTC	1 cut(s)	630
PshBI	ATTAAT	1 cut(s)	3069
PsiI	TTATAA	1 cut(s)	1367
Psp1406I	AACGTT	1 cut(s)	585
Psp5II	RGGWCCY	1 cut(s)	2293
Psp6I	CCWGG	2 cut(s)	2896, 3412
PspCI	CACGTG	1 cut(s)	2978
PspGI	CCWGG	2 cut(s)	2896, 3412
PspLI	CGTACG	1 cut(s)	3047
PspPI	GGNCC	3 cut(s)	1185, 1689, 2293
PspPPI	RGGWCCY	1 cut(s)	2293
PstNI	CAGNNNCTG	1 cut(s)	2529
PsuI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3397, 3517
PvuI	CGATCG	2 cut(s)	169, 772
PvuII	CAGCTG	3 cut(s)	2102, 2924, 3173
RgaI	GCGATCGC	1 cut(s)	772
RseI	CAYNNNNRTG	2 cut(s)	564, 789
SapI	GCTCTTC	1 cut(s)	264
Sau96I	GGNCC	3 cut(s)	1185, 1689, 2293
ScaI	AGTACT	3 cut(s)	2216, 3373, 3448
SchI	GAGTC	5 cut(s)	206, 239, 2228, 2535, 3029
ScrFI	CCNGG	2 cut(s)	2898, 3414
SduI	GDGCHC	2 cut(s)	207, 2983
SfaAI	GCGATCGC	1 cut(s)	772
SfaNI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
SfoI	GGCGCC	1 cut(s)	289
SfuI	TTCGAA	1 cut(s)	2595
SgfI	GCGATCGC	1 cut(s)	772
SinI	GGWCC	3 cut(s)	1185, 1689, 2293
SmiMI	CAYNNNNRTG	2 cut(s)	564, 789
SmlI	CTYRAG	4 cut(s)	191, 814, 2868, 3536
SmoI	CTYRAG	4 cut(s)	191, 814, 2868, 3536
SpeI	ACTAGT	2 cut(s)	1049, 3443
SsiI	CCGC	5 cut(s)	177, 285, 330, 680, 3077
SspDI	GGCGCC	1 cut(s)	287
SspI	AATATT	1 cut(s)	1968
SspMI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3444, 3525
StyD4I	CCNGG	2 cut(s)	2896, 3412
StyI	CCWWGG	1 cut(s)	1757
TaaI	ACNGT	7 cut(s)	998, 1008, 1163, 2093, 2384, 2485, 3468
TatI	WGTACW	4 cut(s)	802, 2214, 3371, 3446
TauI	GCSGC	2 cut(s)	288, 332
TscAI	CASTG	3 cut(s)	31, 1003, 2096
TseFI	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3101
Tsp45I	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3101
TspGWI	ACGGA	2 cut(s)	361, 3120
TspRI	CASTG	3 cut(s)	31, 1003, 2096
Van91I	CCANNNNNTGG	2 cut(s)	3338, 3413
Vha464I	CTTAAG	2 cut(s)	191, 3536
VneI	GTGCAC	1 cut(s)	2979
VpaK11BI	GGWCC	3 cut(s)	1185, 1689, 2293
VspI	ATTAAT	1 cut(s)	3069
XapI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3222, 3287
XceI	RCATGY	3 cut(s)	238, 851, 3121
XspI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3444, 3525
ZraI	GACGTC	1 cut(s)	1394
ZrmI	AGTACT	3 cut(s)	2216, 3373, 3448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.