Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

13108546 .. 13120309

Gene Structure 11764 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG086300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3507 bp

ATGCAACGAAGCAAAACGTCAGCCCTAAAGCGCTCCAAGTTCCGGAAATTCCTTGACAACTTCTGCAAAGCAGGGGATTACTTCAGCGCAATCCGGCTGATTCTTCCGATCCTCGATCGGGAGCGCGGTAGCTATGGCCTTAAGGAGTCGGTGCTCGCCACCTGCCTCATCGACGCTCTCGGCATGTCCCGAGACTCCGACGACGCTCTTCGCCTCATCAATTGGCGCAAGGGCGGCGCCAAGACCGGTGCCAATGCCGGCAATTTCGCCCTCGTCGCCGCCGAGGTGCTGCAACGGAGACAGGGACTTAGTTCAGGTGGACTAACGATCAAGGAGTTGAATGACTTGCTTGATCGTTTGGCATCCGGTGAGAACAGGGCAGAGAAGACTTCAATTCTTTCTACTTTGGTACAGAAGACAAATGCACAAGAAATGAAGTGGATCGTCATGATCATTCTTAAAGATCTCAAATTGGGAATTAGTGAAAAGAGCATCTTCCATGAATTTCATCCGGATGCTGAAGACTTGTTTAACGTTACCTGTGACTTGAAATTGGTTTGTGAAAAGCTGAGGGACAGAAGTCAACGACATAAACGACAGGACATTGAAGTTGGAAAAGCTGTGCGCCCGCAGCTAGCTATGAGAGTTGGTGATGCAACTGGTGCATGGAAAAAGTTTCATGGAAAGGAAGTTGTTGTTGAGTGTAAATTCGACGGCGATCGCATCCAAATTCATAAGAATGGAACCGAAGTACATTACTTCTCAAGGAATTTTCTTGATCACTCTGAATATGGACATGCGATGTCAGACATTATTATACAAAATGTTCAGGCCGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTCTGAAGCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCAAAGGCAGCGAGGGATGGACTTGACAGTGATAAACAGTTGTGCTATGTTGCATTCGATATCCTTTACGTTGGAGATACTAGTGTAATTCACGAGAGCTTGAAGGAAAGGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATTTTAGTACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGGGGAACCTTGTTGGTCCCTAATTGTTCATACTCTGGATGATGTCGAGAGGTTTTTTAAAGAAACCATCGAGAACAGAGATGAGGGGATTGTTTTAAAGGATCTTGGTTCTAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTGAAATTGAAGCCGGATTACATACGGGCTGGTTCTGATCTGGATGTTCTTATAATAGGAGGATACTATGGTTCAGGACGTCGTGGAGGGGAAGTAGCTCAGTTCTTGGTGGGTCTTGCTGAACGTCCAGTTGCAAATACATATCCGAGAAGGTTTGTATCCTTTTGCAGAGTGGGTACTGGACTTTCTGACGAGGAGTTAGATGCTGTGGCAACCAAATTAAAGCCTTATTTAAGGAAGTATGACTACCCCAAAAAGGCACCACCAAGTTTTTATGAAGTAACGAACCATTCAAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATCCTTTCCATAACAAGTGACATCCGAACCATAAGGTCCGAGGTTTTTGCTGCACCATACAGCCTGAGATTTCCACGTATTGATCGAATTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAATCATTTATAGAGCTTGTAGATTCAGGCAATGGTACCACACAAAAGGGGACAGATTATGGTGGCCTGCAGGATGATAAAAAGAAACACAAGAGATCCTCTAAAAAGGAAGAGAAAAGAAATCTATCTCTTGTTCCCTCTCATTTAGTTCAGACTGATGTTTCTGGTGTCAAGGAGGACAGCTTAATATTTTCAAAAATGATGTTCTACTTTGCTAATGTGCCTCCAACTCATTCTCTTGATTCCTTGCACAAAATTGTTGCTGAGAATGGGGGTACTTTTTCGATGAATTTGAATAACTCAGTCACACACTGTGTTGCAGCTGATAGCAAAGGGATTAAATATCAGGCAGCAAAACGTCAGGGAGATGTGATTCATTTGTCTTGGGTCTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCTTTACAGCCAAAGTACTACCTCTTCATTTCTGACTCTTCAAAGAAGAAGTTAGAAGAAGAAATAGATGAGTTTTCTGACCCGTACTTCTGGGACCTTGACCTTGCAGACATTAAGCAGCTTCTAAGCAATGTGCCTAGATCAGAGGATACCAAACGTATCAACTATTACAAGAAAAAATACTGTCCTATGGAGAAATGGTCTCGATTTCACGGCTGCTGCATTTACTTTAACTCATCAATTCATTCCTTGAAACCGGATTGGGAAGTCGTGTTAGGAGTTACAGTAAGAAGGTTAAAGATTGAAGTTCTCATGGGTGGTGGCAGAGTCAGTGACAATCTTGCTGATGCCACCCATTTGGTACTCTTGTCAATTCAGGGGTGCGATGTGGGATTCGAATCCTTATTGCAAAGTTTGACTGAAGCAGAGAAACACTTTTTGCATAGCAAAAGGTTGCATATTGTTGGACTGCAATGGTTGGAAGACTGCTTGGAAAAGAACAAAAGACTGCAAGAGGAATCATATAGTTTAAAACCCTATGGGTGGGAAGAATTCAACATCGAAGAGTGCAAACCTGACATGGTTTTAGAAGAAGCTCCCTGTGACAATGTGGAAATCCAGAAGGTTCCTTCTCTTTCTAACAAAGGGAAAAACAGAGGCAATGCAGCTCAAGAAAACTATGGGATCTTGGGCGCACCTGAGAGGGGTGGCAAAAGGAAAAGAGGAAGACCAGCTGGCACAAACACGAACAGAGGGAAAACAGGTGCCACGCAAGCTCGAAGAACACGTGCACGTATTGGCAGGAAGCCTGCTAAAATATCTGGCAATGAGTCAGATGAAAGTGGTTCTCATGATCGTACGTCATTTAAGGAAGAGGTTAATATCGCGGAAGGGAATCATGGGATGGTAGGTGACGGAAGTTCAGACATGCAAAGAAATGAGGCAATAAAAGATTCTACATCATTACAAAGTGGTAAGGCAGCTGAACAAGAGGTTGTTGGAGACATTAGATTCGATGGGCATTCTGATAAAATTCCTGAAATTGAAATGATTGAGAAGCATAATGGCCAAGACAGAGATAAGCCAGAGAAACTCGAATTTATGGCTGATCCAGTTCAAGCCATGTTGTTTGATCTGATTCCAAGTCTTGGAAAAAAGAATGCTCAAACTATGAATGTGAGTACTGAGGATGAGAAGCCACCCGTAGATCTGAATCCAAGTCCTGGAATGAAGAATGTTCAAACCACGCATACTAGTACTGAGGATGAGAAGCCACCGTTTGATATGGGTGCAGAGCCTACAAAGAAAAAGAAAGTTAGCTATAAGGATCTTGCTAGTCAGCTACTAAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1168

Amino Acids

131.81

Weight (kDa)

7.9

Isoelectric Point (pI)

43.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	5 - 170	1.9e-33	DNA ligase N terminus
DNA_ligase_A_M	PF01068	210 - 425	3e-43	ATP dependent DNA ligase domain
RNA_ligase	PF09414	229 - 424	2.6e-06	RNA ligase
DNA_ligase_A_C	PF04679	449 - 568	2.4e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	637 - 709	1.1e-06	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	639 - 720	3.3e-12	BRCT domain
PTCB-BRCT	PF12738	654 - 698	8e-09	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1316
AarI	CACCTGC	1 cut(s)	170
AatII	GACGTC	1 cut(s)	1345
Acc36I	ACCTGC	1 cut(s)	170
Acc65I	GGTACC	1 cut(s)	1763
AccB1I	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2912
AccB7I	CCANNNNNTGG	2 cut(s)	3296, 3371
AccII	CGCG	2 cut(s)	126, 3035
AccIII	TCCGGA	2 cut(s)	42, 511
AciI	CCGC	5 cut(s)	126, 234, 279, 629, 3035
AclI	AACGTT	1 cut(s)	534
AclWI	GGATC	7 cut(s)	103, 449, 1227, 1818, 2840, 3251, 3483
AcoI	YGGCCR	1 cut(s)	3214
AcsI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2699, 3180, 3245
AcuI	CTGAAG	5 cut(s)	67, 540, 902, 1101, 2589
AcvI	CACGTG	1 cut(s)	2936
AcyI	GRCGYC	2 cut(s)	237, 1342
AdeI	CACNNNGTG	1 cut(s)	2042
AfeI	AGCGCT	1 cut(s)	32
AfiI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2691, 2852, 3296, 3371
AflII	CTTAAG	1 cut(s)	140
AflIII	ACRYGT	1 cut(s)	2933
AgeI	ACCGGT	1 cut(s)	245
AhlI	ACTAGT	2 cut(s)	998, 3401
AjnI	CCWGG	1 cut(s)	3370
Alw21I	GWGCWC	2 cut(s)	156, 2941
Alw26I	GTCTC	5 cut(s)	186, 292, 1558, 2355, 3144
Alw44I	GTGCAC	1 cut(s)	2937
AlwI	GGATC	7 cut(s)	103, 449, 1227, 1818, 2840, 3251, 3483
Ama87I	CYCGRG	1 cut(s)	189
Aor13HI	TCCGGA	2 cut(s)	42, 511
Aor51HI	AGCGCT	1 cut(s)	32
AoxI	GGCC	5 cut(s)	136, 831, 1096, 1792, 3214
ApaLI	GTGCAC	1 cut(s)	2937
ApoI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2699, 3180, 3245
ArsI	GACNNNNNNTTYG	4 cut(s)	1557, 1589, 3143, 3175
AsiGI	ACCGGT	1 cut(s)	245
AsiSI	GCGATCGC	1 cut(s)	721
Asp718I	GGTACC	1 cut(s)	1763
AspLEI	GCGC	7 cut(s)	33, 89, 126, 228, 239, 627, 2843
AspS9I	GGNCC	3 cut(s)	1134, 1638, 2242
AsuHPI	GGTGA	5 cut(s)	380, 662, 866, 1100, 3071
AsuII	TTCGAA	1 cut(s)	2544
AsuNHI	GCTAGC	1 cut(s)	634
AvaI	CYCGRG	1 cut(s)	189
AvaII	GGWCC	3 cut(s)	1134, 1638, 2242
BaeGI	GKGCMC	1 cut(s)	2941
BaeI	ACNNNNGTAYC	6 cut(s)	987, 1020, 1318, 1351, 2501, 2534
BalI	TGGCCA	1 cut(s)	3216
BanI	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2912
BarI	GAAGNNNNNNTAC	6 cut(s)	1265, 1297, 1493, 1525, 2156, 2188
BauI	CACGAG	1 cut(s)	1010
BbrPI	CACGTG	1 cut(s)	2936
BbsI	GAAGAC	5 cut(s)	392, 422, 528, 2637, 2881
Bbv12I	GWGCWC	2 cut(s)	156, 2941
BbvCI	CCTCAGC	1 cut(s)	569
BccI	CCATC	5 cut(s)	845, 929, 1193, 3046, 3158
BceAI	ACGGC	2 cut(s)	730, 2377
BcgI	CGANNNNNNTGC	2 cut(s)	1631, 1665
BciT130I	CCWGG	1 cut(s)	3372
BciVI	GTATCC	3 cut(s)	1319, 1432, 2290
BclI	TGATCA	2 cut(s)	450, 778
BcoDI	GTCTC	5 cut(s)	186, 292, 1558, 2355, 3144
BcuI	ACTAGT	2 cut(s)	998, 3401
BfaI	CTAG	6 cut(s)	635, 999, 1242, 2286, 3402, 3483
BfmI	CTRYAG	1 cut(s)	1796
BfoI	RGCGCY	2 cut(s)	34, 240
BfrI	CTTAAG	1 cut(s)	140
BfuAI	ACCTGC	1 cut(s)	170
BfuI	GTATCC	3 cut(s)	1319, 1432, 2290
BglII	AGATCT	2 cut(s)	463, 3355
BmcAI	AGTACT	3 cut(s)	2165, 3331, 3406
Bme1390I	CCNGG	1 cut(s)	3372
Bme18I	GGWCC	3 cut(s)	1134, 1638, 2242
BmeT110I	CYCGRG	1 cut(s)	189
BmgT120I	GGNCC	3 cut(s)	1134, 1638, 2242
BmrFI	CCNGG	1 cut(s)	3372
BmsI	GCATC	7 cut(s)	371, 501, 505, 643, 732, 1456, 2485
BmtI	GCTAGC	1 cut(s)	638
BoxI	GACNNNNGTC	1 cut(s)	579
BpiI	GAAGAC	5 cut(s)	392, 422, 528, 2637, 2881
BpmI	CTGGAG	1 cut(s)	1025
Bpu10I	CCTNAGC	1 cut(s)	569
Bpu14I	TTCGAA	1 cut(s)	2544
BpuEI	CTTGAG	2 cut(s)	748, 2802
BsaAI	YACGTR	3 cut(s)	1679, 2936, 2942
BsaBI	GATNNNNATC	2 cut(s)	2545, 3360
BsaHI	GRCGYC	2 cut(s)	237, 1342
BsaI	GGTCTC	2 cut(s)	1558, 2355
BsaJI	CCNNGG	3 cut(s)	282, 1641, 1706
BsaWI	WCCGGW	5 cut(s)	42, 245, 365, 511, 2404
BsaXI	ACNNNNNCTCC	6 cut(s)	1341, 1371, 2333, 2363, 2418, 2448
Bsc4I	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2691, 2852, 3296, 3371
Bse118I	RCCGGY	2 cut(s)	245, 257
Bse1I	ACTGG	4 cut(s)	664, 1391, 1447, 3260
Bse3DI	GCAATG	5 cut(s)	1765, 2284, 2627, 2815, 2980
Bse8I	GATNNNNATC	2 cut(s)	2545, 3360
BseAI	TCCGGA	2 cut(s)	42, 511
BseBI	CCWGG	1 cut(s)	3372
BseDI	CCNNGG	3 cut(s)	282, 1641, 1706
BseJI	GATNNNNATC	2 cut(s)	2545, 3360
BseLI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2691, 2852, 3296, 3371
BseMI	GCAATG	5 cut(s)	1765, 2284, 2627, 2815, 2980
BseNI	ACTGG	4 cut(s)	664, 1391, 1447, 3260
BseRI	GAGGAG	1 cut(s)	1472
BseSI	GKGCMC	1 cut(s)	2941
BsgI	GTGCAG	2 cut(s)	1638, 3459
Bsh1236I	CGCG	2 cut(s)	126, 3035
Bsh1285I	CGRYCG	2 cut(s)	118, 721
BshFI	GGCC	5 cut(s)	138, 833, 1098, 1794, 3216
BshNI	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2912
BshTI	ACCGGT	1 cut(s)	245
BsiEI	CGRYCG	2 cut(s)	118, 721
BsiHKAI	GWGCWC	2 cut(s)	156, 2941
BsiHKCI	CYCGRG	1 cut(s)	189
BsiSI	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2405
BsiWI	CGTACG	1 cut(s)	3005
BslFI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2255
BslI	CCNNNNNNNGG	9 cut(s)	42, 118, 1064, 1094, 1519, 2691, 2852, 3296, 3371
BsmAI	GTCTC	5 cut(s)	186, 292, 1558, 2355, 3144
BsmFI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2255
BsmI	GAATGC	4 cut(s)	971, 1721, 3169, 3313
BsnI	GGCC	5 cut(s)	138, 833, 1098, 1794, 3216
Bso31I	GGTCTC	2 cut(s)	1558, 2355
BsoBI	CYCGRG	1 cut(s)	189
Bsp119I	TTCGAA	1 cut(s)	2544
Bsp1286I	GDGCHC	2 cut(s)	156, 2941
Bsp13I	TCCGGA	2 cut(s)	42, 511
BspACI	CCGC	5 cut(s)	126, 234, 279, 629, 3035
BspANI	GGCC	5 cut(s)	138, 833, 1098, 1794, 3216
BspEI	TCCGGA	2 cut(s)	42, 511
BspFNI	CGCG	2 cut(s)	126, 3035
BspHI	TCATGA	2 cut(s)	447, 2998
BspMAI	CTGCAG	1 cut(s)	1800
BspMI	ACCTGC	1 cut(s)	170
BspOI	GCTAGC	1 cut(s)	638
BspPI	GGATC	7 cut(s)	103, 449, 1227, 1818, 2840, 3251, 3483
BspQI	GCTCTTC	1 cut(s)	213
BspT104I	TTCGAA	1 cut(s)	2544
BspT107I	GGYRCC	5 cut(s)	236, 248, 1522, 1763, 2912
BspTI	CTTAAG	1 cut(s)	140
BspTNI	GGTCTC	2 cut(s)	1558, 2355
BsrDI	GCAATG	5 cut(s)	1765, 2284, 2627, 2815, 2980
BsrFI	RCCGGY	2 cut(s)	245, 257
BsrI	ACTGG	4 cut(s)	664, 1391, 1447, 3260
BssAI	RCCGGY	2 cut(s)	245, 257
BssECI	CCNNGG	3 cut(s)	282, 1641, 1706
BssNI	GRCGYC	2 cut(s)	237, 1342
BssSI	CACGAG	1 cut(s)	1010
BssT1I	CCWWGG	1 cut(s)	1706
Bst2BI	CACGAG	1 cut(s)	1010
Bst2UI	CCWGG	1 cut(s)	3372
Bst4CI	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2333, 2434, 3426
Bst6I	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2706, 3015
BstACI	GRCGYC	2 cut(s)	237, 1342
BstAFI	CTTAAG	1 cut(s)	140
BstAPI	GCANNNNNTGC	1 cut(s)	662
BstBAI	YACGTR	3 cut(s)	1679, 2936, 2942
BstBI	TTCGAA	1 cut(s)	2544
BstC8I	GCNNGC	8 cut(s)	156, 259, 629, 636, 1796, 2884, 2922, 2958
BstFNI	CGCG	2 cut(s)	126, 3035
BstH2I	RGCGCY	2 cut(s)	34, 240
BstHHI	GCGC	7 cut(s)	33, 89, 126, 228, 239, 627, 2843
BstMAI	GTCTC	5 cut(s)	186, 292, 1558, 2355, 3144
BstMCI	CGRYCG	2 cut(s)	118, 721
BstMWI	GCNNNNNNNGC	6 cut(s)	234, 275, 631, 662, 926, 2921
BstNI	CCWGG	1 cut(s)	3372
BstNSI	RCATGY	3 cut(s)	187, 800, 3079
BstPAI	GACNNNNGTC	1 cut(s)	579
BstSCI	CCNGG	1 cut(s)	3370
BstSFI	CTRYAG	1 cut(s)	1796
BstSLI	GKGCMC	1 cut(s)	2941
BstUI	CGCG	2 cut(s)	126, 3035
BstV2I	GAAGAC	5 cut(s)	392, 422, 528, 2637, 2881
BstX2I	RGATCY	6 cut(s)	463, 1219, 1823, 2832, 3355, 3475
BstXI	CCANNNNNNTGG	2 cut(s)	1575, 2506
BstYI	RGATCY	6 cut(s)	463, 1219, 1823, 2832, 3355, 3475
BsuI	GTATCC	3 cut(s)	1319, 1432, 2290
BsuRI	GGCC	5 cut(s)	138, 833, 1098, 1794, 3216
BtgZI	GCGATG	2 cut(s)	815, 2547
BtsIMutI	CAGTG	3 cut(s)	952, 2038, 2485
BveI	ACCTGC	1 cut(s)	170
Cac8I	GCNNGC	8 cut(s)	156, 259, 629, 636, 1796, 2884, 2922, 2958
CciI	TCATGA	2 cut(s)	447, 2998
CfoI	GCGC	7 cut(s)	33, 89, 126, 228, 239, 627, 2843
Cfr10I	RCCGGY	2 cut(s)	245, 257
Cfr13I	GGNCC	3 cut(s)	1134, 1638, 2242
CseI	GACGC	2 cut(s)	182, 212
CspAI	ACCGGT	1 cut(s)	245
DinI	GGCGCC	1 cut(s)	238
DraI	TTTAAA	3 cut(s)	1177, 1215, 2679
DraIII	CACNNNGTG	1 cut(s)	2042
EaeI	YGGCCR	1 cut(s)	3214
Eam1104I	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2706, 3015
EarI	CTCTTC	6 cut(s)	213, 1833, 2177, 2191, 2706, 3015
Eco130I	CCWWGG	1 cut(s)	1706
Eco31I	GGTCTC	2 cut(s)	1558, 2355
Eco32I	GATATC	1 cut(s)	979
Eco47I	GGWCC	3 cut(s)	1134, 1638, 2242
Eco47III	AGCGCT	1 cut(s)	32
Eco57I	CTGAAG	5 cut(s)	67, 540, 902, 1101, 2589
Eco72I	CACGTG	1 cut(s)	2936
Eco88I	CYCGRG	1 cut(s)	189
EcoO109I	RGGNCCY	1 cut(s)	2242
EcoRI	GAATTC	1 cut(s)	2699
EcoRII	CCWGG	1 cut(s)	3370
EcoRV	GATATC	1 cut(s)	979
EcoT14I	CCWWGG	1 cut(s)	1706
EgeI	GGCGCC	1 cut(s)	238
EheI	GGCGCC	1 cut(s)	238
ErhI	CCWWGG	1 cut(s)	1706
FalI	AAGNNNNNCTT	2 cut(s)	479, 511
FaqI	GGGAC	7 cut(s)	172, 318, 587, 884, 1120, 1792, 2255
FauI	CCCGC	1 cut(s)	636
FbaI	TGATCA	2 cut(s)	450, 778
FspBI	CTAG	6 cut(s)	635, 999, 1242, 2286, 3402, 3483
GlaI	GCGC	7 cut(s)	32, 88, 125, 227, 238, 626, 2842
GsuI	CTGGAG	1 cut(s)	1025
HaeII	RGCGCY	2 cut(s)	34, 240
HaeIII	GGCC	5 cut(s)	138, 833, 1098, 1794, 3216
HapII	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2405
HgaI	GACGC	2 cut(s)	182, 212
HhaI	GCGC	7 cut(s)	33, 89, 126, 228, 239, 627, 2843
Hin1I	GRCGYC	2 cut(s)	237, 1342
Hin6I	GCGC	7 cut(s)	31, 87, 124, 226, 237, 625, 2841
HinP1I	GCGC	7 cut(s)	31, 87, 124, 226, 237, 625, 2841
HincII	GTYRAC	1 cut(s)	584
HindII	GTYRAC	1 cut(s)	584
HpaII	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2405
HphI	GGTGA	5 cut(s)	380, 662, 866, 1100, 3071
Hpy166II	GTNNAC	3 cut(s)	320, 584, 2939
Hpy8I	GTNNAC	3 cut(s)	320, 584, 2939
Hpy99I	CGWCG	6 cut(s)	176, 203, 206, 278, 716, 1347
HpyAV	CCTTC	7 cut(s)	1015, 1407, 2156, 2433, 2764, 2787, 3032
HpyCH4III	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2333, 2434, 3426
HpyF10VI	GCNNNNNNNGC	6 cut(s)	234, 275, 631, 662, 926, 2921
Hsp92I	GRCGYC	2 cut(s)	237, 1342
HspAI	GCGC	7 cut(s)	31, 87, 124, 226, 237, 625, 2841
KasI	GGCGCC	1 cut(s)	236
Kpn2I	TCCGGA	2 cut(s)	42, 511
KpnI	GGTACC	1 cut(s)	1767
KroI	GCCGGC	1 cut(s)	257
KroNI	GCCGGC	1 cut(s)	259
Ksp22I	TGATCA	2 cut(s)	450, 778
LguI	GCTCTTC	1 cut(s)	213
LmnI	GCTCC	3 cut(s)	38, 121, 2749
LweI	GCATC	7 cut(s)	371, 501, 505, 643, 732, 1456, 2485
MaeI	CTAG	6 cut(s)	635, 999, 1242, 2286, 3402, 3483
MaeIII	GTNAC	9 cut(s)	535, 542, 1543, 1619, 2032, 2428, 2480, 2750, 3059
MfeI	CAATTG	1 cut(s)	220
MflI	RGATCY	6 cut(s)	463, 1219, 1823, 2832, 3355, 3475
MhlI	GDGCHC	2 cut(s)	156, 2941
MlsI	TGGCCA	1 cut(s)	3216
MluNI	TGGCCA	1 cut(s)	3216
Mly113I	GGCGCC	1 cut(s)	237
MlyI	GAGTC	5 cut(s)	155, 188, 2177, 2484, 2987
MmeI	TCCRAC	7 cut(s)	222, 592, 970, 1979, 2593, 2607, 3127
Mox20I	TGGCCA	1 cut(s)	3216
MroI	TCCGGA	2 cut(s)	42, 511
MroNI	GCCGGC	1 cut(s)	257
MscI	TGGCCA	1 cut(s)	3216
MslI	CAYNNNNRTG	2 cut(s)	513, 738
Msp20I	TGGCCA	1 cut(s)	3216
MspA1I	CMGCKG	3 cut(s)	2051, 2882, 3131
MspCI	CTTAAG	1 cut(s)	140
MspI	CCGG	8 cut(s)	43, 94, 246, 258, 366, 512, 1277, 2405
MspR9I	CCNGG	1 cut(s)	3372
MunI	CAATTG	1 cut(s)	220
Mva1269I	GAATGC	4 cut(s)	971, 1721, 3169, 3313
MvaI	CCWGG	1 cut(s)	3372
MvnI	CGCG	2 cut(s)	126, 3035
MwoI	GCNNNNNNNGC	6 cut(s)	234, 275, 631, 662, 926, 2921
NaeI	GCCGGC	1 cut(s)	259
NarI	GGCGCC	1 cut(s)	237
NgoMIV	GCCGGC	1 cut(s)	257
NheI	GCTAGC	1 cut(s)	634
NmeAIII	GCCGAG	2 cut(s)	159, 307
NmuCI	GTSAC	6 cut(s)	542, 1619, 2032, 2480, 2750, 3059
NspI	RCATGY	3 cut(s)	187, 800, 3079
NspV	TTCGAA	1 cut(s)	2544
PagI	TCATGA	2 cut(s)	447, 2998
PaqCI	CACCTGC	1 cut(s)	170
PciSI	GCTCTTC	1 cut(s)	213
PcsI	WCGNNNNNNNCGW	4 cut(s)	279, 592, 1684, 2932
PctI	GAATGC	4 cut(s)	971, 1721, 3169, 3313
PdiI	GCCGGC	1 cut(s)	259
Pfl23II	CGTACG	1 cut(s)	3005
PflMI	CCANNNNNTGG	2 cut(s)	3296, 3371
PfoI	TCCNGGA	1 cut(s)	3370
PinAI	ACCGGT	1 cut(s)	245
Ple19I	CGATCG	2 cut(s)	118, 721
PleI	GAGTC	5 cut(s)	154, 188, 2177, 2483, 2986
PluTI	GGCGCC	1 cut(s)	240
PmaCI	CACGTG	1 cut(s)	2936
PmlI	CACGTG	1 cut(s)	2936
PpsI	GAGTC	5 cut(s)	154, 188, 2177, 2483, 2986
Ppu21I	YACGTR	3 cut(s)	1679, 2936, 2942
PpuMI	RGGWCCY	1 cut(s)	2242
PshAI	GACNNNNGTC	1 cut(s)	579
PsiI	TTATAA	1 cut(s)	1316
Psp1406I	AACGTT	1 cut(s)	534
Psp5II	RGGWCCY	1 cut(s)	2242
Psp6I	CCWGG	1 cut(s)	3370
PspCI	CACGTG	1 cut(s)	2936
PspGI	CCWGG	1 cut(s)	3370
PspLI	CGTACG	1 cut(s)	3005
PspPI	GGNCC	3 cut(s)	1134, 1638, 2242
PspPPI	RGGWCCY	1 cut(s)	2242
PstI	CTGCAG	1 cut(s)	1800
PsuI	RGATCY	6 cut(s)	463, 1219, 1823, 2832, 3355, 3475
PvuI	CGATCG	2 cut(s)	118, 721
PvuII	CAGCTG	3 cut(s)	2051, 2882, 3131
RgaI	GCGATCGC	1 cut(s)	721
RseI	CAYNNNNRTG	2 cut(s)	513, 738
SapI	GCTCTTC	1 cut(s)	213
Sau96I	GGNCC	3 cut(s)	1134, 1638, 2242
SbfI	CCTGCAGG	1 cut(s)	1800
ScaI	AGTACT	3 cut(s)	2165, 3331, 3406
SchI	GAGTC	5 cut(s)	155, 188, 2177, 2484, 2987
ScrFI	CCNGG	1 cut(s)	3372
SdaI	CCTGCAGG	1 cut(s)	1800
SduI	GDGCHC	2 cut(s)	156, 2941
SfaAI	GCGATCGC	1 cut(s)	721
SfaNI	GCATC	7 cut(s)	371, 501, 505, 643, 732, 1456, 2485
SfcI	CTRYAG	1 cut(s)	1796
SfoI	GGCGCC	1 cut(s)	238
SfuI	TTCGAA	1 cut(s)	2544
SgfI	GCGATCGC	1 cut(s)	721
SinI	GGWCC	3 cut(s)	1134, 1638, 2242
SmiMI	CAYNNNNRTG	2 cut(s)	513, 738
SmlI	CTYRAG	3 cut(s)	140, 763, 2817
SmoI	CTYRAG	3 cut(s)	140, 763, 2817
SpeI	ACTAGT	2 cut(s)	998, 3401
Sse8387I	CCTGCAGG	1 cut(s)	1800
SsiI	CCGC	5 cut(s)	126, 234, 279, 629, 3035
SspDI	GGCGCC	1 cut(s)	236
SspI	AATATT	1 cut(s)	1917
SspMI	CTAG	6 cut(s)	635, 999, 1242, 2286, 3402, 3483
StyD4I	CCNGG	1 cut(s)	3370
StyI	CCWWGG	1 cut(s)	1706
TaaI	ACNGT	7 cut(s)	947, 957, 1112, 2042, 2333, 2434, 3426
TatI	WGTACW	4 cut(s)	751, 2163, 3329, 3404
TauI	GCSGC	2 cut(s)	237, 281
TscAI	CASTG	3 cut(s)	952, 2045, 2485
TseFI	GTSAC	6 cut(s)	542, 1619, 2032, 2480, 2750, 3059
Tsp45I	GTSAC	6 cut(s)	542, 1619, 2032, 2480, 2750, 3059
TspGWI	ACGGA	2 cut(s)	310, 3078
TspRI	CASTG	3 cut(s)	952, 2045, 2485
Van91I	CCANNNNNTGG	2 cut(s)	3296, 3371
Vha464I	CTTAAG	1 cut(s)	140
VneI	GTGCAC	1 cut(s)	2937
VpaK11BI	GGWCC	3 cut(s)	1134, 1638, 2242
XapI	RAATTY	9 cut(s)	47, 503, 707, 729, 769, 2017, 2699, 3180, 3245
XceI	RCATGY	3 cut(s)	187, 800, 3079
XspI	CTAG	6 cut(s)	635, 999, 1242, 2286, 3402, 3483
ZraI	GACGTC	1 cut(s)	1343
ZrmI	AGTACT	3 cut(s)	2165, 3331, 3406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.