Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

16595461 .. 16606988

Gene Structure 11528 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G007730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3558 bp

ATGAATAAGGAGGAGACCAAGTTCAGTGTGCTGTGCAGCCTCTTCAATTGGATGCAACGAAGCAAAACGTCAGCCCTAAAGCGCTCCAAGTTCCGGAAATTCCTTGACAACTTCTGCAAAGCAGGGGATTACTTCAGCGCAATCCGGCTGATTCTTCCGAGCCTCGATCGGGAGCGCGGCAGCTATGGCCTTAAGGAGTCGGTGCTCGCCACCTGCCTCATCGACGCTCTCGGCATGTCCCGAGACTCCGACGACGCTCTTCGCCTCATCAATTGGCGCAAGGGCGGCGCCAAGACCGGTGCCAATGCCGGCAATTTCGCCCTCGTCGCCGCCGAGGTGCTGCAACGGAGACAGGGACTTAGTTCAGGTGGACTAACGATTAAGGAGTTGAATGACTTGCTTGATCGTTTGGCATCCGGTGAAAATAGGGCAGAGAAGACTTCAATTCTTTCTACTTTGGTACAGAAGACAAATGCACAAGAAATGAAGTGGATCGTCATGATCATTCTTAAAGATCTCAAATTGGGAATTAGTGAAAAGAGCATCTTCCATGAATTTCATCCGGATGCTGAAGACTTGTTTAATGTTACCTGTGACTTGAAATTGGTTTGTGAAAAGCTGAGGGACAGAAGTCAACGACATAAACGACAGGACATTGAAGTTGGAAAAGCTGTGCGCCCGCAGCTAGCTATGAGAGTTGGTGATGCAACTGGTGCATGGAAAAAGTTTCATGGAAAGGAAGTTGTTGTTGAGTGTAAATTCGACGGCGATCGCATCCAAATTCATAAGAATGGAACTGAAGTACATTACTTCTCAAGGAATTTTCTTGATCACTCTGAATATGGACATGCAATGTCAGACATTATTATACAAAATGTTCAGGCTGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTCTGAAGCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCAAAGGCAGCGAGGGATGGACTTGACAGTGATAAACAGTTGTGCTATGTTGCATTCGATATCCTTTACGTTGGAGATACTAGTGTAATTCACGAGAGCTTGAAGGAAAGGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATTTTAGTACCCAATGGTGGCCTTAACACTCATCATTCTTCAGGGGAACCTTGTTGGTCCCTAATTGTTCATACTCTGGATGATGTCGAGAGGTTTTTTAAAGAAACCATCGAGAACAGAGATGAGGGGATTGTTTTAAAGGATCTTGGTTCTAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTGAAATTGAAGCCAGATTACATACAGGCTGGTTCTGATCTGGATGTCCTTATAATAGGAGGATACTATGGCTCAGGACGTCGTGGAGGGGAAGTAGCTCAGTTCTTGGTGGGTCTTGCTGAACGTCCTGTTGCAAATACATATCCGAGAAGGTTTGTATCCTTTTGCAGAGTGGGTACTGGACTTTCTGATGAGGAGTTAGATGCTGTGGCAACCAAATTAAAGCCTTATTTAAGGAAGTATGACTACCCCAAAAAGGCACCACCAAGTTTTTATGAAGTAACGAACCATTCAAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATCCTTTCCATAACAAGTGACATCCGAACCATAAGGTCCGAGGTTTTTGCCGCACCATACAGCCTGAGATTTCCACGTATTGATCGAATTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAATCATTTATAGAGCTTGTAGATTCAGGCAATGGTACCACACAAAAGGGGACAGATTATGGTGGCCTGCAGGATGATAAAAAGAAACACAAGAGATCCTCTAAAAAGGAAGAGAAAAGAAATCTATCTCTTGTTCCCTCTCATTTAGTTCAGACTGATGTTTCTGGTGTCAAGGAGGACAGCTTAATATTTTCAAAAATGATGTTCTACTTTGCTAATGTGCCTCCAACTCATTCTCTTGATTCCTTGCACAAAATTGTTGCTGAGAATGGGGGTACTTTTTCGATGAATTTGAATAACTCAGTCACACACTGTGTTGCAGCTGATAGCAAAGGGATTAAATATCAGGCAGCAAAACGTCAGGGAGATGTGATTCATTTGTCTTGGGTCTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCTTTACAGCCAAAGTACTACCTCTTCATTTCTGACTCTTCAAAGAAGAAGTTAGAAGAAGAAATAGATGAGTTTTCTGACCCGTACTTCTGGGACCTTGACCTTGCAGACATTAAGCAGCTTCTAAGCAATGTGCCTAGATCAGAGGATACCAAACGTATCAACTATTACAAGAAAAAATACTGTCCTATGGAGAAATGGTCTCGATTTCACGGCTGCTGCATTTACTTTAACTCATCAATTCATTCCTTGAAACCGGATTGGGAAGTCGTGTTAGGAGTTACAGTAAGAAGGTTAAAGATTGAAGTTCTCATGGGTGGTGGCAGAGTCAGTGACAATCTTGCTGATGCCACCCATTTGGTACTCTTGTCAATTCAGGGGTGCGATGTGGGATTCGAATCCTTATTGCAAAGTTTGACTGAAGCAGAGAAACACTTTTTGCATAGCAAAAGGTTGCATATTGTTGGACTGCAATGGTTGGAAGACAGCTTGGAAAAGAACAAAAGACTGCATGAGGAATCATATAGTTTAAAACCCTATGGCTGGGAAGAATTCAACATCGAAGAGTGCAAACCTGACATGGTTTTAGAAGAAGCTCCCTGTGACAATGTGGAAATCCAGAAGGTTCCTTCTCTTTCTAACAAAGGGAAAAACAGAGGCAATGCAGCTCAAGAAAACTATGGGATCTTGGGCGCACCTGAGAGGGGTGGCAAAAGGAAAAGAGGAAGACCAGCTGGCACAAACACGAACAGAGGGAAAACAGGTGCCACGCAAGCTCGAAGAACACGTGCACGTATTGGCAGGAAGCCTGCTAAAATATCTGGGAATGAGTCAGATGAAAGTGGTTCTCATGATCGTACGTCATTTAAGGAAGAGGTTAATATCGCGGAAGGGAATCATGGGATGGTAGGTGACGGAAGTTCAGACATGCAAAGAAATGAGGCAATAAAAGATTCTACATCATTACAAAGTGGTAAGGCAGCTGAACAAGAGGTTGCTGGAGATATTAGATTCGATGGCCATTCTGATAAAATTCCTGAAATTGAAATGATTGAGAAGCATAATGGCCAAGACAGAGCTAAGGCAGAGAAACTCGAATTTATGGCTGATCCAGTTCAAGCCATGTTGTTTGATCTGATTCCAAGTCTTGGAAAAAAGAATGTTCAAACTATGAATGTGAGTCCTGAGGATGAGAAGCCACCCGTAGATCTGAATCCAAGTCCAGGAATGAAGAATGTTCAAACCACGCATACTAGTACTGAGGATGAGAAGCCACCGTTTGATCTGGGTGCAGAGCCTCCAAAGAAAAAGAAAGTTAGCTATAAGGATCTTGCTAGTCAGCTACTTAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1185

Amino Acids

133.69

Weight (kDa)

7.74

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	7 - 187	5.3e-38	DNA ligase N terminus
DNA_ligase_A_M	PF01068	227 - 442	3.6e-43	ATP dependent DNA ligase domain
RNA_ligase	PF09414	246 - 441	2.3e-06	RNA ligase
DNA_ligase_A_C	PF04679	466 - 585	2.5e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	654 - 726	1.2e-06	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	656 - 737	3.3e-12	BRCT domain
PTCB-BRCT	PF12738	671 - 715	8.1e-09	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1367
AarI	CACCTGC	1 cut(s)	221
AatII	GACGTC	1 cut(s)	1396
Acc36I	ACCTGC	1 cut(s)	221
Acc65I	GGTACC	1 cut(s)	1814
AccB1I	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2963
AccB7I	CCANNNNNTGG	1 cut(s)	3347
AccII	CGCG	2 cut(s)	177, 3086
AccIII	TCCGGA	2 cut(s)	93, 562
AciI	CCGC	6 cut(s)	177, 285, 330, 680, 1704, 3086
AclWI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3302, 3534
AcoI	YGGCCR	2 cut(s)	3217, 3265
AcsI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3231, 3296
AcuI	CTGAAG	6 cut(s)	118, 591, 819, 953, 1152, 2640
AcvI	CACGTG	1 cut(s)	2987
AcyI	GRCGYC	2 cut(s)	288, 1393
AdeI	CACNNNGTG	1 cut(s)	2093
AfeI	AGCGCT	1 cut(s)	83
AfiI	CCNNNNNNNGG	9 cut(s)	93, 169, 1115, 1145, 1570, 2742, 2903, 3347, 3422
AflII	CTTAAG	2 cut(s)	191, 3545
AflIII	ACRYGT	1 cut(s)	2984
AgeI	ACCGGT	1 cut(s)	296
AhlI	ACTAGT	2 cut(s)	1049, 3452
AjnI	CCWGG	1 cut(s)	3421
AloI	GAACNNNNNNTCC	1 cut(s)	37
Alw21I	GWGCWC	2 cut(s)	207, 2992
Alw26I	GTCTC	5 cut(s)	8, 237, 343, 1609, 2406
Alw44I	GTGCAC	1 cut(s)	2988
AlwI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3302, 3534
Ama87I	CYCGRG	1 cut(s)	240
Aor13HI	TCCGGA	2 cut(s)	93, 562
Aor51HI	AGCGCT	1 cut(s)	83
AoxI	GGCC	5 cut(s)	187, 1147, 1843, 3217, 3265
ApaLI	GTGCAC	1 cut(s)	2988
ApoI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3231, 3296
ArsI	GACNNNNNNTTYG	2 cut(s)	1608, 1640
AsiGI	ACCGGT	1 cut(s)	296
AsiSI	GCGATCGC	1 cut(s)	772
Asp718I	GGTACC	1 cut(s)	1814
AspLEI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
AspS9I	GGNCC	3 cut(s)	1185, 1689, 2293
AsuHPI	GGTGA	4 cut(s)	431, 713, 917, 3122
AsuII	TTCGAA	1 cut(s)	2595
AsuNHI	GCTAGC	1 cut(s)	685
AvaI	CYCGRG	1 cut(s)	240
AvaII	GGWCC	3 cut(s)	1185, 1689, 2293
AxyI	CCTNAGG	1 cut(s)	3384
BaeGI	GKGCMC	1 cut(s)	2992
BaeI	ACNNNNGTAYC	4 cut(s)	1038, 1071, 2552, 2585
BalI	TGGCCA	2 cut(s)	3219, 3267
BanI	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2963
BarI	GAAGNNNNNNTAC	6 cut(s)	1316, 1348, 1544, 1576, 2207, 2239
BauI	CACGAG	1 cut(s)	1061
BbrPI	CACGTG	1 cut(s)	2987
BbsI	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2932
Bbv12I	GWGCWC	2 cut(s)	207, 2992
BbvCI	CCTCAGC	1 cut(s)	620
BccI	CCATC	5 cut(s)	896, 980, 1244, 3097, 3209
BceAI	ACGGC	2 cut(s)	781, 2428
BcgI	CGANNNNNNTGC	2 cut(s)	1682, 1716
BciT130I	CCWGG	1 cut(s)	3423
BciVI	GTATCC	3 cut(s)	1370, 1483, 2341
BclI	TGATCA	2 cut(s)	501, 829
BcoDI	GTCTC	5 cut(s)	8, 237, 343, 1609, 2406
BcuI	ACTAGT	2 cut(s)	1049, 3452
BfaI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3453, 3534
BfmI	CTRYAG	1 cut(s)	1847
BfoI	RGCGCY	2 cut(s)	85, 291
BfrI	CTTAAG	2 cut(s)	191, 3545
BfuAI	ACCTGC	1 cut(s)	221
BfuI	GTATCC	3 cut(s)	1370, 1483, 2341
BglII	AGATCT	2 cut(s)	514, 3406
BmcAI	AGTACT	2 cut(s)	2216, 3457
Bme1390I	CCNGG	1 cut(s)	3423
Bme18I	GGWCC	3 cut(s)	1185, 1689, 2293
BmeT110I	CYCGRG	1 cut(s)	240
BmgT120I	GGNCC	3 cut(s)	1185, 1689, 2293
BmrFI	CCNGG	1 cut(s)	3423
BmsI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
BmtI	GCTAGC	1 cut(s)	689
BoxI	GACNNNNGTC	1 cut(s)	630
BpiI	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2932
BpmI	CTGGAG	2 cut(s)	1076, 3219
Bpu10I	CCTNAGC	3 cut(s)	620, 1387, 3279
Bpu14I	TTCGAA	1 cut(s)	2595
BpuEI	CTTGAG	2 cut(s)	799, 2853
BsaAI	YACGTR	3 cut(s)	1730, 2987, 2993
BsaBI	GATNNNNATC	2 cut(s)	2596, 3411
BsaHI	GRCGYC	2 cut(s)	288, 1393
BsaI	GGTCTC	3 cut(s)	8, 1609, 2406
BsaJI	CCNNGG	3 cut(s)	333, 1692, 1757
BsaWI	WCCGGW	5 cut(s)	93, 296, 416, 562, 2455
BsaXI	ACNNNNNCTCC	8 cut(s)	5, 35, 1392, 1422, 2384, 2414, 2469, 2499
Bsc4I	CCNNNNNNNGG	9 cut(s)	93, 169, 1115, 1145, 1570, 2742, 2903, 3347, 3422
Bse118I	RCCGGY	2 cut(s)	296, 308
Bse1I	ACTGG	3 cut(s)	715, 1498, 3311
Bse21I	CCTNAGG	1 cut(s)	3384
Bse3DI	GCAATG	5 cut(s)	858, 1816, 2335, 2678, 2866
Bse8I	GATNNNNATC	2 cut(s)	2596, 3411
BseAI	TCCGGA	2 cut(s)	93, 562
BseBI	CCWGG	1 cut(s)	3423
BseDI	CCNNGG	3 cut(s)	333, 1692, 1757
BseJI	GATNNNNATC	2 cut(s)	2596, 3411
BseLI	CCNNNNNNNGG	9 cut(s)	93, 169, 1115, 1145, 1570, 2742, 2903, 3347, 3422
BseMI	GCAATG	5 cut(s)	858, 1816, 2335, 2678, 2866
BseNI	ACTGG	3 cut(s)	715, 1498, 3311
BseRI	GAGGAG	2 cut(s)	26, 1523
BseSI	GKGCMC	1 cut(s)	2992
BseYI	CCCAGC	1 cut(s)	2742
BsgI	GTGCAG	2 cut(s)	55, 3510
Bsh1236I	CGCG	2 cut(s)	177, 3086
Bsh1285I	CGRYCG	2 cut(s)	169, 772
BshFI	GGCC	5 cut(s)	189, 1149, 1845, 3219, 3267
BshNI	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2963
BshTI	ACCGGT	1 cut(s)	296
BsiEI	CGRYCG	2 cut(s)	169, 772
BsiHKAI	GWGCWC	2 cut(s)	207, 2992
BsiHKCI	CYCGRG	1 cut(s)	240
BsiSI	CCGG	7 cut(s)	94, 145, 297, 309, 417, 563, 2456
BsiWI	CGTACG	1 cut(s)	3056
BslFI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
BslI	CCNNNNNNNGG	9 cut(s)	93, 169, 1115, 1145, 1570, 2742, 2903, 3347, 3422
BsmAI	GTCTC	5 cut(s)	8, 237, 343, 1609, 2406
BsmFI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
BsmI	GAATGC	2 cut(s)	1022, 1772
BsnI	GGCC	5 cut(s)	189, 1149, 1845, 3219, 3267
Bso31I	GGTCTC	3 cut(s)	8, 1609, 2406
BsoBI	CYCGRG	1 cut(s)	240
Bsp119I	TTCGAA	1 cut(s)	2595
Bsp1286I	GDGCHC	2 cut(s)	207, 2992
Bsp13I	TCCGGA	2 cut(s)	93, 562
BspACI	CCGC	6 cut(s)	177, 285, 330, 680, 1704, 3086
BspANI	GGCC	5 cut(s)	189, 1149, 1845, 3219, 3267
BspEI	TCCGGA	2 cut(s)	93, 562
BspFNI	CGCG	2 cut(s)	177, 3086
BspHI	TCATGA	2 cut(s)	498, 3049
BspMAI	CTGCAG	1 cut(s)	1851
BspMI	ACCTGC	1 cut(s)	221
BspOI	GCTAGC	1 cut(s)	689
BspPI	GGATC	6 cut(s)	500, 1278, 1869, 2891, 3302, 3534
BspQI	GCTCTTC	1 cut(s)	264
BspT104I	TTCGAA	1 cut(s)	2595
BspT107I	GGYRCC	5 cut(s)	287, 299, 1573, 1814, 2963
BspTI	CTTAAG	2 cut(s)	191, 3545
BspTNI	GGTCTC	3 cut(s)	8, 1609, 2406
BsrDI	GCAATG	5 cut(s)	858, 1816, 2335, 2678, 2866
BsrFI	RCCGGY	2 cut(s)	296, 308
BsrI	ACTGG	3 cut(s)	715, 1498, 3311
BssAI	RCCGGY	2 cut(s)	296, 308
BssECI	CCNNGG	3 cut(s)	333, 1692, 1757
BssNI	GRCGYC	2 cut(s)	288, 1393
BssSI	CACGAG	1 cut(s)	1061
BssT1I	CCWWGG	1 cut(s)	1757
Bst2BI	CACGAG	1 cut(s)	1061
Bst2UI	CCWGG	1 cut(s)	3423
Bst4CI	ACNGT	6 cut(s)	998, 1008, 2093, 2384, 2485, 3477
Bst6I	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3066
BstACI	GRCGYC	2 cut(s)	288, 1393
BstAFI	CTTAAG	2 cut(s)	191, 3545
BstAPI	GCANNNNNTGC	1 cut(s)	713
BstBAI	YACGTR	3 cut(s)	1730, 2987, 2993
BstBI	TTCGAA	1 cut(s)	2595
BstC8I	GCNNGC	8 cut(s)	207, 310, 680, 687, 1847, 2935, 2973, 3009
BstFNI	CGCG	2 cut(s)	177, 3086
BstH2I	RGCGCY	2 cut(s)	85, 291
BstHHI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
BstMAI	GTCTC	5 cut(s)	8, 237, 343, 1609, 2406
BstMCI	CGRYCG	2 cut(s)	169, 772
BstMWI	GCNNNNNNNGC	7 cut(s)	186, 285, 326, 682, 713, 977, 2972
BstNI	CCWGG	1 cut(s)	3423
BstNSI	RCATGY	3 cut(s)	238, 851, 3130
BstPAI	GACNNNNGTC	1 cut(s)	630
BstSCI	CCNGG	1 cut(s)	3421
BstSFI	CTRYAG	1 cut(s)	1847
BstSLI	GKGCMC	1 cut(s)	2992
BstUI	CGCG	2 cut(s)	177, 3086
BstV2I	GAAGAC	5 cut(s)	443, 473, 579, 2688, 2932
BstX2I	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3406, 3526
BstXI	CCANNNNNNTGG	2 cut(s)	1626, 2557
BstYI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3406, 3526
Bsu36I	CCTNAGG	1 cut(s)	3384
BsuI	GTATCC	3 cut(s)	1370, 1483, 2341
BsuRI	GGCC	5 cut(s)	189, 1149, 1845, 3219, 3267
BtgZI	GCGATG	1 cut(s)	2598
BtsIMutI	CAGTG	4 cut(s)	31, 1003, 2089, 2536
BveI	ACCTGC	1 cut(s)	221
Cac8I	GCNNGC	8 cut(s)	207, 310, 680, 687, 1847, 2935, 2973, 3009
CciI	TCATGA	2 cut(s)	498, 3049
CfoI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
Cfr10I	RCCGGY	2 cut(s)	296, 308
Cfr13I	GGNCC	3 cut(s)	1185, 1689, 2293
CseI	GACGC	2 cut(s)	233, 263
CspAI	ACCGGT	1 cut(s)	296
DinI	GGCGCC	1 cut(s)	289
DraI	TTTAAA	3 cut(s)	1228, 1266, 2730
DraIII	CACNNNGTG	1 cut(s)	2093
EaeI	YGGCCR	2 cut(s)	3217, 3265
Eam1104I	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3066
EarI	CTCTTC	7 cut(s)	47, 264, 1884, 2228, 2242, 2757, 3066
Eco130I	CCWWGG	1 cut(s)	1757
Eco31I	GGTCTC	3 cut(s)	8, 1609, 2406
Eco32I	GATATC	1 cut(s)	1030
Eco47I	GGWCC	3 cut(s)	1185, 1689, 2293
Eco47III	AGCGCT	1 cut(s)	83
Eco57I	CTGAAG	6 cut(s)	118, 591, 819, 953, 1152, 2640
Eco72I	CACGTG	1 cut(s)	2987
Eco81I	CCTNAGG	1 cut(s)	3384
Eco88I	CYCGRG	1 cut(s)	240
EcoO109I	RGGNCCY	1 cut(s)	2293
EcoRI	GAATTC	1 cut(s)	2750
EcoRII	CCWGG	1 cut(s)	3421
EcoRV	GATATC	1 cut(s)	1030
EcoT14I	CCWWGG	1 cut(s)	1757
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	1 cut(s)	1757
FalI	AAGNNNNNCTT	2 cut(s)	530, 562
FaqI	GGGAC	7 cut(s)	223, 369, 638, 935, 1171, 1843, 2306
FauI	CCCGC	1 cut(s)	687
FbaI	TGATCA	2 cut(s)	501, 829
FspBI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3453, 3534
GlaI	GCGC	7 cut(s)	83, 139, 176, 278, 289, 677, 2893
GsaI	CCCAGC	1 cut(s)	2746
GsuI	CTGGAG	2 cut(s)	1076, 3219
HaeII	RGCGCY	2 cut(s)	85, 291
HaeIII	GGCC	5 cut(s)	189, 1149, 1845, 3219, 3267
HapII	CCGG	7 cut(s)	94, 145, 297, 309, 417, 563, 2456
HgaI	GACGC	2 cut(s)	233, 263
HhaI	GCGC	7 cut(s)	84, 140, 177, 279, 290, 678, 2894
Hin1I	GRCGYC	2 cut(s)	288, 1393
Hin6I	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
HinP1I	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
HincII	GTYRAC	1 cut(s)	635
HindII	GTYRAC	1 cut(s)	635
HpaII	CCGG	7 cut(s)	94, 145, 297, 309, 417, 563, 2456
HphI	GGTGA	4 cut(s)	431, 713, 917, 3122
Hpy166II	GTNNAC	3 cut(s)	371, 635, 2990
Hpy8I	GTNNAC	3 cut(s)	371, 635, 2990
Hpy99I	CGWCG	6 cut(s)	227, 254, 257, 329, 767, 1398
HpyAV	CCTTC	7 cut(s)	1066, 1458, 2207, 2484, 2815, 2838, 3083
HpyCH4III	ACNGT	6 cut(s)	998, 1008, 2093, 2384, 2485, 3477
HpyF10VI	GCNNNNNNNGC	7 cut(s)	186, 285, 326, 682, 713, 977, 2972
Hsp92I	GRCGYC	2 cut(s)	288, 1393
HspAI	GCGC	7 cut(s)	82, 138, 175, 277, 288, 676, 2892
KasI	GGCGCC	1 cut(s)	287
Kpn2I	TCCGGA	2 cut(s)	93, 562
KpnI	GGTACC	1 cut(s)	1818
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
Ksp22I	TGATCA	2 cut(s)	501, 829
LguI	GCTCTTC	1 cut(s)	264
LmnI	GCTCC	3 cut(s)	89, 172, 2800
LweI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
MaeI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3453, 3534
MaeIII	GTNAC	9 cut(s)	586, 593, 1594, 1670, 2083, 2479, 2531, 2801, 3110
MfeI	CAATTG	2 cut(s)	46, 271
MflI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3406, 3526
MhlI	GDGCHC	2 cut(s)	207, 2992
MlsI	TGGCCA	2 cut(s)	3219, 3267
MluNI	TGGCCA	2 cut(s)	3219, 3267
Mly113I	GGCGCC	1 cut(s)	288
MlyI	GAGTC	6 cut(s)	206, 239, 2228, 2535, 3038, 3388
MmeI	TCCRAC	6 cut(s)	273, 643, 1021, 2030, 2644, 2658
Mox20I	TGGCCA	2 cut(s)	3219, 3267
MroI	TCCGGA	2 cut(s)	93, 562
MroNI	GCCGGC	1 cut(s)	308
MscI	TGGCCA	2 cut(s)	3219, 3267
MslI	CAYNNNNRTG	2 cut(s)	564, 789
Msp20I	TGGCCA	2 cut(s)	3219, 3267
MspA1I	CMGCKG	3 cut(s)	2102, 2933, 3182
MspCI	CTTAAG	2 cut(s)	191, 3545
MspI	CCGG	7 cut(s)	94, 145, 297, 309, 417, 563, 2456
MspR9I	CCNGG	1 cut(s)	3423
MunI	CAATTG	2 cut(s)	46, 271
Mva1269I	GAATGC	2 cut(s)	1022, 1772
MvaI	CCWGG	1 cut(s)	3423
MvnI	CGCG	2 cut(s)	177, 3086
MwoI	GCNNNNNNNGC	7 cut(s)	186, 285, 326, 682, 713, 977, 2972
NaeI	GCCGGC	1 cut(s)	310
NarI	GGCGCC	1 cut(s)	288
NgoMIV	GCCGGC	1 cut(s)	308
NheI	GCTAGC	1 cut(s)	685
NmeAIII	GCCGAG	2 cut(s)	210, 358
NmuCI	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3110
NspI	RCATGY	3 cut(s)	238, 851, 3130
NspV	TTCGAA	1 cut(s)	2595
PagI	TCATGA	2 cut(s)	498, 3049
PaqCI	CACCTGC	1 cut(s)	221
PciSI	GCTCTTC	1 cut(s)	264
PcsI	WCGNNNNNNNCGW	4 cut(s)	330, 643, 1735, 2983
PctI	GAATGC	2 cut(s)	1022, 1772
PdiI	GCCGGC	1 cut(s)	310
Pfl23II	CGTACG	1 cut(s)	3056
PflMI	CCANNNNNTGG	1 cut(s)	3347
PfoI	TCCNGGA	1 cut(s)	3421
PinAI	ACCGGT	1 cut(s)	296
Ple19I	CGATCG	2 cut(s)	169, 772
PleI	GAGTC	6 cut(s)	205, 239, 2228, 2534, 3037, 3387
PluTI	GGCGCC	1 cut(s)	291
PmaCI	CACGTG	1 cut(s)	2987
PmlI	CACGTG	1 cut(s)	2987
PpsI	GAGTC	6 cut(s)	205, 239, 2228, 2534, 3037, 3387
Ppu21I	YACGTR	3 cut(s)	1730, 2987, 2993
PpuMI	RGGWCCY	1 cut(s)	2293
PshAI	GACNNNNGTC	1 cut(s)	630
PsiI	TTATAA	1 cut(s)	1367
Psp5II	RGGWCCY	1 cut(s)	2293
Psp6I	CCWGG	1 cut(s)	3421
PspCI	CACGTG	1 cut(s)	2987
PspFI	CCCAGC	1 cut(s)	2742
PspGI	CCWGG	1 cut(s)	3421
PspLI	CGTACG	1 cut(s)	3056
PspPI	GGNCC	3 cut(s)	1185, 1689, 2293
PspPPI	RGGWCCY	1 cut(s)	2293
PstI	CTGCAG	1 cut(s)	1851
PsuI	RGATCY	6 cut(s)	514, 1270, 1874, 2883, 3406, 3526
PvuI	CGATCG	2 cut(s)	169, 772
PvuII	CAGCTG	3 cut(s)	2102, 2933, 3182
RgaI	GCGATCGC	1 cut(s)	772
RseI	CAYNNNNRTG	2 cut(s)	564, 789
SapI	GCTCTTC	1 cut(s)	264
Sau96I	GGNCC	3 cut(s)	1185, 1689, 2293
SbfI	CCTGCAGG	1 cut(s)	1851
ScaI	AGTACT	2 cut(s)	2216, 3457
SchI	GAGTC	6 cut(s)	206, 239, 2228, 2535, 3038, 3388
ScrFI	CCNGG	1 cut(s)	3423
SdaI	CCTGCAGG	1 cut(s)	1851
SduI	GDGCHC	2 cut(s)	207, 2992
SfaAI	GCGATCGC	1 cut(s)	772
SfaNI	GCATC	8 cut(s)	42, 422, 552, 556, 694, 783, 1507, 2536
SfcI	CTRYAG	1 cut(s)	1847
SfoI	GGCGCC	1 cut(s)	289
SfuI	TTCGAA	1 cut(s)	2595
SgfI	GCGATCGC	1 cut(s)	772
SinI	GGWCC	3 cut(s)	1185, 1689, 2293
SmiMI	CAYNNNNRTG	2 cut(s)	564, 789
SmlI	CTYRAG	4 cut(s)	191, 814, 2868, 3545
SmoI	CTYRAG	4 cut(s)	191, 814, 2868, 3545
SpeI	ACTAGT	2 cut(s)	1049, 3452
Sse8387I	CCTGCAGG	1 cut(s)	1851
SsiI	CCGC	6 cut(s)	177, 285, 330, 680, 1704, 3086
SspDI	GGCGCC	1 cut(s)	287
SspI	AATATT	1 cut(s)	1968
SspMI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3453, 3534
StyD4I	CCNGG	1 cut(s)	3421
StyI	CCWWGG	1 cut(s)	1757
TaaI	ACNGT	6 cut(s)	998, 1008, 2093, 2384, 2485, 3477
TatI	WGTACW	3 cut(s)	802, 2214, 3455
TauI	GCSGC	4 cut(s)	180, 288, 332, 1706
TscAI	CASTG	4 cut(s)	31, 1003, 2096, 2536
TseFI	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3110
Tsp45I	GTSAC	6 cut(s)	593, 1670, 2083, 2531, 2801, 3110
TspGWI	ACGGA	2 cut(s)	361, 3129
TspRI	CASTG	4 cut(s)	31, 1003, 2096, 2536
Van91I	CCANNNNNTGG	1 cut(s)	3347
Vha464I	CTTAAG	2 cut(s)	191, 3545
VneI	GTGCAC	1 cut(s)	2988
VpaK11BI	GGWCC	3 cut(s)	1185, 1689, 2293
XapI	RAATTY	9 cut(s)	98, 554, 758, 780, 820, 2068, 2750, 3231, 3296
XceI	RCATGY	3 cut(s)	238, 851, 3130
XspI	CTAG	6 cut(s)	686, 1050, 1293, 2337, 3453, 3534
ZraI	GACGTC	1 cut(s)	1394
ZrmI	AGTACT	2 cut(s)	2216, 3457

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.