Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Forward (+)

50128466 .. 50140135

Gene Structure 11670 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009274

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3564 bp

ATGAATAAGGAAGAGGAGGAGACCAAGTTCAGTGTGCTGTGCAGCCTCTTCAATTGGATGCAACGAAGCAAAACGTCAGCCCTAAAGCGCTCCAAGTTCCGGAAATTCCTTGACAACTTCTGCAAAGCAGGGGATTACTTCAGCGCAATCCGGCTGATTCTTCCGAACCTCGATCGGGAGCGCGGTAGCTATGGCCTTAAGGAGTCGGTGCTCGCCACCTGCCTCATCGACGCTCTCGGCATGTCCCGAGACTCCGACGACGCTCTTCGCCTCATCAATTGGCGCAAGGGCGGCGCCAAGACCGGTGCCAATGCCGGCAACTTCGCCCTCGTCGCCGCCGAGGTGCTGCAACGGAGACAGGGACTTAGTTCAGGTGGACTAACGATTAAGGAGTTGAATGACTTGCTTGATCGTTTGGCATCCGGTGAAAATAGGGCAGAGAAGACTTCAATTCTTTCTACTTTGGTACAGAAGACAAATGCACAAGAAATGAAGTGGATCGTCATGATCATTCTTAAAGATCTCAAATTGGGAATTAGTGAAAAGAGCATCTTCCATGAATTTCATCCGGATGCTGAAGACTTGTTTAATGTTACCTGTGACTTGAAATTGGTTTGTGAAAAGCTGAGGGACAGAAGTCAACGACATAAACGACAGGACATTGAAGTTGGAAAAGCCGTGCGCCCGCAGCTAGCTATGAGAGTTGGTGATGCAACTGGTGCATGGAAAAAGTTTCATGGAAAGGAAGTTGTTGTTGAGTGTAAATTCGACGGCGATCGCATCCAAATTCATAAGAATGGAACCGAAGTACATTACTTCTCAAGGAATTTTCTTGATCACTCTGAATATGGACATGCAATGTCAGACATTATTATACAAAATGTTCAGGCCGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACAACTCTGAAGCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCAAAGGCAGCGAGGGATGGACTTGACAGTGACAAACAGTTGTGCTATGTTGCATTCGATATCCTTTACGTTGGAGATACTAGTGTAATTCACGAGAGCTTGAAGGAAAGGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATTTTAGTACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGGGGAACCTTGTTGGTCCCTAATTGTTCATACTCTGGATGATGTCGAGAGGTTTTTTAAAGAAACCATCGAGAACAGAGATGAGGGGATTGTTTTAAAGGATCTTGGTTCTAAATGGGAACCTAGTGATCGTAGTGGAAAGTGGTTGAAATTGAAGCCGGATTACATACGTGCTGGTTCTGATCTGGATGTTCTTATAATAGGAGGATACTATGGTTCAGGACGTCGTGGAGGGGAAGTAGCTCAGTTCTTGGTGGGTCTTGCTGAACGTCCAGTTGCAAATACATATCCGAGAAGGTTTGTATCCTTTTGCAGAGTGGGTACTGGACTTTCTGACGAGGAGTTAGATGCTGTGGCAACCAAATTAAAGCCTTATTTAAGGAAGTATGACTACCCCAAAAAGGCACCACCAAGTTTTTATGAAGTAACGAACCATTCAAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATCCTTTCCATAACAAGTGACATCCGAACCATAAGGTCTGAGGTTTTTGCTGCACCATACAGCCTGAGATTTCCACGTATTGATCGAATTAGATATGATAAACCTTGGCATGAATGCCTTGATGTGCAATCATTTATAGAGCTTGTAGATTCAGGCAATGGTACCACACAAAAGGGGACAGATTATGGTGGCCTGCAGGATGATAAAAAGAAACACAAGAGATCCTCTAAAAAGGAAGAGAAAAGAAATCTATCTCTTGTTCCCTCTCATTTAGTTCAGACTGATGTTTCTGGTGTCAAGGAGGACAGCTTAATATTTTCGAAAATGATGTTCTACTTTGCTAATGTGCCTCCAACTCATTCTCTTGATTCCTTGCACAAAATTGTTGCTGAGAATGGGGGTACTTTTTCGATGAATTTGAATAACTCAGTCACACACTGTGTTGCAGCTGACAGCAAAGGGATTAAATATCAGGCAGCAAAACGTCAGGGAGATGTGATTCATTTGTCATGGGTCTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCTTTACAGCCAAAGTACTACCTCTTCATTTCTGACTCTTCAAAGAAGAAGTTAGAAGAAGAAATAGATGAGTTTTCTGACCCGTACTTCTGGGACCTTGACCTTGCAGACATTAAGCAGCTTCTAAGCAATGTGCCTAGATCAGAGGATACCAAACGTATCAACTATTACAAGAAAAAATACTGTCCTATGGAGAAATGGTCTCGATTTCACGGCTGCTGCATTTACTTTAACTCATCAATTCATTCCTTGAAACCGGATTGGGAAGTCGTGTTAGGAGTTACAGTAAGAAGGTTAAAGATTGAAGTTCTCATGGGTGGTGGCAGAGTCAGTGACAATCTTGCGGATGCCACCCATTTGGTACTCTTGTCAATTCAGGGGTGCGATGTGGGATTCGAATCCTTATTGCAAAGTTTGACTGAAGCAGAGAAACACTTTTTGCATAGCAAAAGGTTGCATATTGTTGGACTGCAATGGTTGGAAGACTGCTTGGAAAAGAACAAAAGACTGCATGAGGAATCATATAGTTTAAAACCCAACGGGTGGGAAGAATTCAACATCAAAGAGTGCAAACCTGACATGGTTTTAGAAGAAGCTCCCTGTGACAACGTGGAAATCCAGAAGGTTCCTTCTCTTTCTAACAAAGGAAAAAACAGAGGCAATGCAGTGCAAGAAAACTATGGGATCTTGGGCGCACCTGAGAGGGGTGGCAAAAGGAAAAGAGGAAGACCAGCTGGCACAAACACGAACAGAGGGAAAACAGGTGCCACGCAAGCTCGAAGAACACGTGCACGTATTGGCAGGAAGCGTGCTAAAATATCTGGCAATGAGTCAGATGAAAGTGGTTCTCATGATCGTACGTCATTTAAGGAAGAGGTTAATATTGCGGAAGGGAATCATGGGATGGTAGGTGATGGAAGTTCAGACATGCAAAGAAATGAGGCAATAAAAGATTCTACATCATTACAAAGTGGTAAGGCAGCTGAACAAGAGGTTGCTGGAGACATTAGATTCGATGGGCATTCTGATAAAATTCCTGAAATTGAAATGATTGAGAAGCATAATGGCCAAGACAGAGCTAAGCCGGAGAAACTCGAATTTATGGCTGATCCAGTTCAAGCCATGTTGTTTGATCTGATTCCAAGTCTAGGAAAAAAGAGTGCTCTGACTATGAATATGAGTACTGAGGATGAGAAGCTACCCGTAGATCTGAATCCAAGTCCTGGAATGAAGAATGTTCAAACCACGCATACTAGTACTGAGGATGAGAAGCCACCATTTGATATGGGTGCAGAGCGTACAAAGAAAAAGAAAGTTAGCTATAAGGATCTTGCTAGTCAGCTACTTAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1188

Amino Acids

134.18

Weight (kDa)

8.22

Isoelectric Point (pI)

42.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	9 - 189	4.4e-38	DNA ligase N terminus
DNA_ligase_A_M	PF01068	229 - 444	3.2e-43	ATP dependent DNA ligase domain
RNA_ligase	PF09414	248 - 443	4.2e-06	RNA ligase
DNA_ligase_A_C	PF04679	468 - 587	2.5e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	656 - 728	1.2e-06	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	658 - 739	3.4e-12	BRCT domain
PTCB-BRCT	PF12738	673 - 717	8.1e-09	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1373
AarI	CACCTGC	1 cut(s)	227
AatII	GACGTC	1 cut(s)	1402
Acc36I	ACCTGC	1 cut(s)	227
Acc65I	GGTACC	1 cut(s)	1820
AccB1I	GGYRCC	5 cut(s)	293, 305, 1579, 1820, 2969
AccB7I	CCANNNNNTGG	2 cut(s)	2748, 3428
AccII	CGCG	1 cut(s)	183
AccIII	TCCGGA	2 cut(s)	99, 568
AciI	CCGC	6 cut(s)	183, 291, 336, 686, 2549, 3092
AclWI	GGATC	6 cut(s)	506, 1284, 1875, 2897, 3308, 3540
AcoI	YGGCCR	1 cut(s)	3271
AcsI	RAATTY	9 cut(s)	104, 560, 764, 786, 826, 2074, 2756, 3237, 3302
AcuI	CTGAAG	5 cut(s)	124, 597, 959, 1158, 2646
AcvI	CACGTG	1 cut(s)	2993
AcyI	GRCGYC	2 cut(s)	294, 1399
AdeI	CACNNNGTG	1 cut(s)	2099
AfeI	AGCGCT	1 cut(s)	89
AfiI	CCNNNNNNNGG	9 cut(s)	99, 175, 1121, 1151, 1576, 2748, 2909, 3353, 3428
AflII	CTTAAG	2 cut(s)	197, 3551
AflIII	ACRYGT	1 cut(s)	2990
AgeI	ACCGGT	1 cut(s)	302
AhlI	ACTAGT	2 cut(s)	1055, 3458
AjnI	CCWGG	1 cut(s)	3427
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
Alw21I	GWGCWC	3 cut(s)	213, 2998, 3370
Alw26I	GTCTC	6 cut(s)	14, 243, 349, 1615, 2412, 3201
Alw44I	GTGCAC	1 cut(s)	2994
AlwI	GGATC	6 cut(s)	506, 1284, 1875, 2897, 3308, 3540
Ama87I	CYCGRG	1 cut(s)	246
Aor13HI	TCCGGA	2 cut(s)	99, 568
Aor51HI	AGCGCT	1 cut(s)	89
AoxI	GGCC	5 cut(s)	193, 888, 1153, 1849, 3271
ApaLI	GTGCAC	1 cut(s)	2994
ApoI	RAATTY	9 cut(s)	104, 560, 764, 786, 826, 2074, 2756, 3237, 3302
ArsI	GACNNNNNNTTYG	4 cut(s)	1614, 1646, 3200, 3232
AsiGI	ACCGGT	1 cut(s)	302
AsiSI	GCGATCGC	1 cut(s)	778
Asp718I	GGTACC	1 cut(s)	1820
AspLEI	GCGC	7 cut(s)	90, 146, 183, 285, 296, 684, 2900
AspS9I	GGNCC	2 cut(s)	1191, 2299
AsuHPI	GGTGA	5 cut(s)	437, 719, 923, 1157, 3128
AsuII	TTCGAA	2 cut(s)	1979, 2601
AsuNHI	GCTAGC	1 cut(s)	691
AvaI	CYCGRG	1 cut(s)	246
AvaII	GGWCC	2 cut(s)	1191, 2299
BaeGI	GKGCMC	1 cut(s)	2998
BaeI	ACNNNNGTAYC	6 cut(s)	1044, 1077, 1375, 1408, 2558, 2591
BalI	TGGCCA	1 cut(s)	3273
BanI	GGYRCC	5 cut(s)	293, 305, 1579, 1820, 2969
BarI	GAAGNNNNNNTAC	6 cut(s)	1322, 1354, 1550, 1582, 2213, 2245
BauI	CACGAG	1 cut(s)	1067
BbrPI	CACGTG	1 cut(s)	2993
BbsI	GAAGAC	5 cut(s)	449, 479, 585, 2694, 2938
Bbv12I	GWGCWC	3 cut(s)	213, 2998, 3370
BbvCI	CCTCAGC	1 cut(s)	626
BccI	CCATC	6 cut(s)	902, 986, 1250, 3103, 3113, 3215
BceAI	ACGGC	3 cut(s)	662, 787, 2434
BciT130I	CCWGG	1 cut(s)	3429
BciVI	GTATCC	3 cut(s)	1376, 1489, 2347
BclI	TGATCA	2 cut(s)	507, 835
BcoDI	GTCTC	6 cut(s)	14, 243, 349, 1615, 2412, 3201
BcuI	ACTAGT	2 cut(s)	1055, 3458
BfaI	CTAG	7 cut(s)	692, 1056, 1299, 2343, 3353, 3459, 3540
BfmI	CTRYAG	1 cut(s)	1853
BfoI	RGCGCY	2 cut(s)	91, 297
BfrI	CTTAAG	2 cut(s)	197, 3551
BfuAI	ACCTGC	1 cut(s)	227
BfuI	GTATCC	3 cut(s)	1376, 1489, 2347
BglII	AGATCT	2 cut(s)	520, 3412
BlpI	GCTNAGC	1 cut(s)	3285
BmcAI	AGTACT	3 cut(s)	2222, 3388, 3463
Bme1390I	CCNGG	1 cut(s)	3429
Bme18I	GGWCC	2 cut(s)	1191, 2299
BmeT110I	CYCGRG	1 cut(s)	246
BmgT120I	GGNCC	2 cut(s)	1191, 2299
BmrFI	CCNGG	1 cut(s)	3429
BmsI	GCATC	8 cut(s)	48, 428, 558, 562, 700, 789, 1513, 2542
BmtI	GCTAGC	1 cut(s)	695
BoxI	GACNNNNGTC	1 cut(s)	636
BpiI	GAAGAC	5 cut(s)	449, 479, 585, 2694, 2938
BpmI	CTGGAG	2 cut(s)	1082, 3225
Bpu10I	CCTNAGC	1 cut(s)	626
Bpu1102I	GCTNAGC	1 cut(s)	3285
Bpu14I	TTCGAA	2 cut(s)	1979, 2601
BpuEI	CTTGAG	1 cut(s)	805
BsaAI	YACGTR	4 cut(s)	1346, 1736, 2993, 2999
BsaBI	GATNNNNATC	2 cut(s)	2602, 3417
BsaHI	GRCGYC	2 cut(s)	294, 1399
BsaI	GGTCTC	3 cut(s)	14, 1615, 2412
BsaJI	CCNNGG	2 cut(s)	339, 1763
BsaWI	WCCGGW	5 cut(s)	99, 302, 422, 568, 2461
BsaXI	ACNNNNNCTCC	8 cut(s)	11, 41, 1398, 1428, 2390, 2420, 2475, 2505
Bsc4I	CCNNNNNNNGG	9 cut(s)	99, 175, 1121, 1151, 1576, 2748, 2909, 3353, 3428
Bse118I	RCCGGY	2 cut(s)	302, 314
Bse1I	ACTGG	4 cut(s)	721, 1448, 1504, 3317
Bse3DI	GCAATG	6 cut(s)	864, 1822, 2341, 2684, 2872, 3037
Bse8I	GATNNNNATC	2 cut(s)	2602, 3417
BseAI	TCCGGA	2 cut(s)	99, 568
BseBI	CCWGG	1 cut(s)	3429
BseDI	CCNNGG	2 cut(s)	339, 1763
BseJI	GATNNNNATC	2 cut(s)	2602, 3417
BseLI	CCNNNNNNNGG	9 cut(s)	99, 175, 1121, 1151, 1576, 2748, 2909, 3353, 3428
BseMI	GCAATG	6 cut(s)	864, 1822, 2341, 2684, 2872, 3037
BseNI	ACTGG	4 cut(s)	721, 1448, 1504, 3317
BseRI	GAGGAG	3 cut(s)	29, 32, 1529
BseSI	GKGCMC	1 cut(s)	2998
BsgI	GTGCAG	3 cut(s)	61, 1695, 3516
Bsh1236I	CGCG	1 cut(s)	183
Bsh1285I	CGRYCG	2 cut(s)	175, 778
BshFI	GGCC	5 cut(s)	195, 890, 1155, 1851, 3273
BshNI	GGYRCC	5 cut(s)	293, 305, 1579, 1820, 2969
BshTI	ACCGGT	1 cut(s)	302
BsiEI	CGRYCG	2 cut(s)	175, 778
BsiHKAI	GWGCWC	3 cut(s)	213, 2998, 3370
BsiHKCI	CYCGRG	1 cut(s)	246
BsiSI	CCGG	9 cut(s)	100, 151, 303, 315, 423, 569, 1334, 2462, 3290
BsiWI	CGTACG	1 cut(s)	3062
BslFI	GGGAC	7 cut(s)	229, 375, 644, 941, 1177, 1849, 2312
BslI	CCNNNNNNNGG	9 cut(s)	99, 175, 1121, 1151, 1576, 2748, 2909, 3353, 3428
BsmAI	GTCTC	6 cut(s)	14, 243, 349, 1615, 2412, 3201
BsmFI	GGGAC	7 cut(s)	229, 375, 644, 941, 1177, 1849, 2312
BsmI	GAATGC	3 cut(s)	1028, 1778, 3226
BsnI	GGCC	5 cut(s)	195, 890, 1155, 1851, 3273
Bso31I	GGTCTC	3 cut(s)	14, 1615, 2412
BsoBI	CYCGRG	1 cut(s)	246
Bsp119I	TTCGAA	2 cut(s)	1979, 2601
Bsp1286I	GDGCHC	3 cut(s)	213, 2998, 3370
Bsp13I	TCCGGA	2 cut(s)	99, 568
Bsp1720I	GCTNAGC	1 cut(s)	3285
BspACI	CCGC	6 cut(s)	183, 291, 336, 686, 2549, 3092
BspANI	GGCC	5 cut(s)	195, 890, 1155, 1851, 3273
BspEI	TCCGGA	2 cut(s)	99, 568
BspFNI	CGCG	1 cut(s)	183
BspHI	TCATGA	2 cut(s)	504, 3055
BspMAI	CTGCAG	1 cut(s)	1857
BspMI	ACCTGC	1 cut(s)	227
BspOI	GCTAGC	1 cut(s)	695
BspPI	GGATC	6 cut(s)	506, 1284, 1875, 2897, 3308, 3540
BspQI	GCTCTTC	1 cut(s)	270
BspT104I	TTCGAA	2 cut(s)	1979, 2601
BspT107I	GGYRCC	5 cut(s)	293, 305, 1579, 1820, 2969
BspTI	CTTAAG	2 cut(s)	197, 3551
BspTNI	GGTCTC	3 cut(s)	14, 1615, 2412
BsrDI	GCAATG	6 cut(s)	864, 1822, 2341, 2684, 2872, 3037
BsrFI	RCCGGY	2 cut(s)	302, 314
BsrI	ACTGG	4 cut(s)	721, 1448, 1504, 3317
BssAI	RCCGGY	2 cut(s)	302, 314
BssECI	CCNNGG	2 cut(s)	339, 1763
BssNI	GRCGYC	2 cut(s)	294, 1399
BssSI	CACGAG	1 cut(s)	1067
BssT1I	CCWWGG	1 cut(s)	1763
Bst2BI	CACGAG	1 cut(s)	1067
Bst2UI	CCWGG	1 cut(s)	3429
Bst4CI	ACNGT	6 cut(s)	1004, 1014, 1169, 2099, 2390, 2491
Bst6I	CTCTTC	7 cut(s)	6, 53, 270, 1890, 2234, 2248, 3072
BstACI	GRCGYC	2 cut(s)	294, 1399
BstAFI	CTTAAG	2 cut(s)	197, 3551
BstAPI	GCANNNNNTGC	1 cut(s)	719
BstBAI	YACGTR	4 cut(s)	1346, 1736, 2993, 2999
BstBI	TTCGAA	2 cut(s)	1979, 2601
BstC8I	GCNNGC	8 cut(s)	213, 316, 686, 693, 1853, 2941, 2979, 3015
BstFNI	CGCG	1 cut(s)	183
BstH2I	RGCGCY	2 cut(s)	91, 297
BstHHI	GCGC	7 cut(s)	90, 146, 183, 285, 296, 684, 2900
BstMAI	GTCTC	6 cut(s)	14, 243, 349, 1615, 2412, 3201
BstMCI	CGRYCG	2 cut(s)	175, 778
BstMWI	GCNNNNNNNGC	6 cut(s)	291, 332, 688, 719, 983, 2978
BstNI	CCWGG	1 cut(s)	3429
BstNSI	RCATGY	3 cut(s)	244, 857, 3136
BstPAI	GACNNNNGTC	1 cut(s)	636
BstSCI	CCNGG	1 cut(s)	3427
BstSFI	CTRYAG	1 cut(s)	1853
BstSLI	GKGCMC	1 cut(s)	2998
BstUI	CGCG	1 cut(s)	183
BstV2I	GAAGAC	5 cut(s)	449, 479, 585, 2694, 2938
BstX2I	RGATCY	6 cut(s)	520, 1276, 1880, 2889, 3412, 3532
BstXI	CCANNNNNNTGG	2 cut(s)	1632, 2563
BstYI	RGATCY	6 cut(s)	520, 1276, 1880, 2889, 3412, 3532
BsuI	GTATCC	3 cut(s)	1376, 1489, 2347
BsuRI	GGCC	5 cut(s)	195, 890, 1155, 1851, 3273
BtgZI	GCGATG	1 cut(s)	2604
BtsI	GCAGTG	1 cut(s)	2877
BtsIMutI	CAGTG	5 cut(s)	37, 1009, 2095, 2542, 2877
BveI	ACCTGC	1 cut(s)	227
Cac8I	GCNNGC	8 cut(s)	213, 316, 686, 693, 1853, 2941, 2979, 3015
CciI	TCATGA	2 cut(s)	504, 3055
CfoI	GCGC	7 cut(s)	90, 146, 183, 285, 296, 684, 2900
Cfr10I	RCCGGY	2 cut(s)	302, 314
Cfr13I	GGNCC	2 cut(s)	1191, 2299
CseI	GACGC	2 cut(s)	239, 269
CspAI	ACCGGT	1 cut(s)	302
DinI	GGCGCC	1 cut(s)	295
DraI	TTTAAA	3 cut(s)	1234, 1272, 2736
DraIII	CACNNNGTG	1 cut(s)	2099
EaeI	YGGCCR	1 cut(s)	3271
Eam1104I	CTCTTC	7 cut(s)	6, 53, 270, 1890, 2234, 2248, 3072
EarI	CTCTTC	7 cut(s)	6, 53, 270, 1890, 2234, 2248, 3072
Eco130I	CCWWGG	1 cut(s)	1763
Eco31I	GGTCTC	3 cut(s)	14, 1615, 2412
Eco32I	GATATC	1 cut(s)	1036
Eco47I	GGWCC	2 cut(s)	1191, 2299
Eco47III	AGCGCT	1 cut(s)	89
Eco57I	CTGAAG	5 cut(s)	124, 597, 959, 1158, 2646
Eco72I	CACGTG	1 cut(s)	2993
Eco88I	CYCGRG	1 cut(s)	246
EcoO109I	RGGNCCY	1 cut(s)	2299
EcoRI	GAATTC	1 cut(s)	2756
EcoRII	CCWGG	1 cut(s)	3427
EcoRV	GATATC	1 cut(s)	1036
EcoT14I	CCWWGG	1 cut(s)	1763
EgeI	GGCGCC	1 cut(s)	295
EheI	GGCGCC	1 cut(s)	295
ErhI	CCWWGG	1 cut(s)	1763
FalI	AAGNNNNNCTT	2 cut(s)	536, 568
FaqI	GGGAC	7 cut(s)	229, 375, 644, 941, 1177, 1849, 2312
FauI	CCCGC	1 cut(s)	693
FbaI	TGATCA	2 cut(s)	507, 835
FspBI	CTAG	7 cut(s)	692, 1056, 1299, 2343, 3353, 3459, 3540
GlaI	GCGC	7 cut(s)	89, 145, 182, 284, 295, 683, 2899
GsuI	CTGGAG	2 cut(s)	1082, 3225
HaeII	RGCGCY	2 cut(s)	91, 297
HaeIII	GGCC	5 cut(s)	195, 890, 1155, 1851, 3273
HapII	CCGG	9 cut(s)	100, 151, 303, 315, 423, 569, 1334, 2462, 3290
HgaI	GACGC	2 cut(s)	239, 269
HhaI	GCGC	7 cut(s)	90, 146, 183, 285, 296, 684, 2900
Hin1I	GRCGYC	2 cut(s)	294, 1399
Hin6I	GCGC	7 cut(s)	88, 144, 181, 283, 294, 682, 2898
HinP1I	GCGC	7 cut(s)	88, 144, 181, 283, 294, 682, 2898
HincII	GTYRAC	1 cut(s)	641
HindII	GTYRAC	1 cut(s)	641
HpaII	CCGG	9 cut(s)	100, 151, 303, 315, 423, 569, 1334, 2462, 3290
HphI	GGTGA	5 cut(s)	437, 719, 923, 1157, 3128
Hpy166II	GTNNAC	3 cut(s)	377, 641, 2996
Hpy8I	GTNNAC	3 cut(s)	377, 641, 2996
Hpy99I	CGWCG	6 cut(s)	233, 260, 263, 335, 773, 1404
HpyAV	CCTTC	7 cut(s)	1072, 1464, 2213, 2490, 2821, 2844, 3089
HpyCH4III	ACNGT	6 cut(s)	1004, 1014, 1169, 2099, 2390, 2491
HpyF10VI	GCNNNNNNNGC	6 cut(s)	291, 332, 688, 719, 983, 2978
Hsp92I	GRCGYC	2 cut(s)	294, 1399
HspAI	GCGC	7 cut(s)	88, 144, 181, 283, 294, 682, 2898
KasI	GGCGCC	1 cut(s)	293
Kpn2I	TCCGGA	2 cut(s)	99, 568
KpnI	GGTACC	1 cut(s)	1824
KroI	GCCGGC	1 cut(s)	314
KroNI	GCCGGC	1 cut(s)	316
Ksp22I	TGATCA	2 cut(s)	507, 835
LguI	GCTCTTC	1 cut(s)	270
LmnI	GCTCC	3 cut(s)	95, 178, 2806
LweI	GCATC	8 cut(s)	48, 428, 558, 562, 700, 789, 1513, 2542
MaeI	CTAG	7 cut(s)	692, 1056, 1299, 2343, 3353, 3459, 3540
MaeIII	GTNAC	9 cut(s)	592, 599, 1004, 1600, 1676, 2089, 2485, 2537, 2807
MfeI	CAATTG	2 cut(s)	52, 277
MflI	RGATCY	6 cut(s)	520, 1276, 1880, 2889, 3412, 3532
MhlI	GDGCHC	3 cut(s)	213, 2998, 3370
MlsI	TGGCCA	1 cut(s)	3273
MluNI	TGGCCA	1 cut(s)	3273
Mly113I	GGCGCC	1 cut(s)	294
MlyI	GAGTC	5 cut(s)	212, 245, 2234, 2541, 3044
MmeI	TCCRAC	6 cut(s)	279, 649, 1027, 2036, 2650, 2664
Mox20I	TGGCCA	1 cut(s)	3273
MroI	TCCGGA	2 cut(s)	99, 568
MroNI	GCCGGC	1 cut(s)	314
MscI	TGGCCA	1 cut(s)	3273
MslI	CAYNNNNRTG	2 cut(s)	570, 795
Msp20I	TGGCCA	1 cut(s)	3273
MspA1I	CMGCKG	3 cut(s)	2108, 2939, 3188
MspCI	CTTAAG	2 cut(s)	197, 3551
MspI	CCGG	9 cut(s)	100, 151, 303, 315, 423, 569, 1334, 2462, 3290
MspR9I	CCNGG	1 cut(s)	3429
MunI	CAATTG	2 cut(s)	52, 277
Mva1269I	GAATGC	3 cut(s)	1028, 1778, 3226
MvaI	CCWGG	1 cut(s)	3429
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	6 cut(s)	291, 332, 688, 719, 983, 2978
NaeI	GCCGGC	1 cut(s)	316
NarI	GGCGCC	1 cut(s)	294
NgoMIV	GCCGGC	1 cut(s)	314
NheI	GCTAGC	1 cut(s)	691
NmeAIII	GCCGAG	2 cut(s)	216, 364
NmuCI	GTSAC	6 cut(s)	599, 1004, 1676, 2089, 2537, 2807
NspI	RCATGY	3 cut(s)	244, 857, 3136
NspV	TTCGAA	2 cut(s)	1979, 2601
PagI	TCATGA	2 cut(s)	504, 3055
PaqCI	CACCTGC	1 cut(s)	227
PciSI	GCTCTTC	1 cut(s)	270
PcsI	WCGNNNNNNNCGW	4 cut(s)	336, 649, 1741, 2989
PctI	GAATGC	3 cut(s)	1028, 1778, 3226
PdiI	GCCGGC	1 cut(s)	316
Pfl23II	CGTACG	1 cut(s)	3062
PflMI	CCANNNNNTGG	2 cut(s)	2748, 3428
PfoI	TCCNGGA	1 cut(s)	3427
PinAI	ACCGGT	1 cut(s)	302
Ple19I	CGATCG	2 cut(s)	175, 778
PleI	GAGTC	5 cut(s)	211, 245, 2234, 2540, 3043
PluTI	GGCGCC	1 cut(s)	297
PmaCI	CACGTG	1 cut(s)	2993
PmlI	CACGTG	1 cut(s)	2993
PpsI	GAGTC	5 cut(s)	211, 245, 2234, 2540, 3043
Ppu21I	YACGTR	4 cut(s)	1346, 1736, 2993, 2999
PpuMI	RGGWCCY	1 cut(s)	2299
PshAI	GACNNNNGTC	1 cut(s)	636
PsiI	TTATAA	1 cut(s)	1373
Psp5II	RGGWCCY	1 cut(s)	2299
Psp6I	CCWGG	1 cut(s)	3427
PspCI	CACGTG	1 cut(s)	2993
PspGI	CCWGG	1 cut(s)	3427
PspLI	CGTACG	1 cut(s)	3062
PspPI	GGNCC	2 cut(s)	1191, 2299
PspPPI	RGGWCCY	1 cut(s)	2299
PstI	CTGCAG	1 cut(s)	1857
PsuI	RGATCY	6 cut(s)	520, 1276, 1880, 2889, 3412, 3532
PvuI	CGATCG	2 cut(s)	175, 778
PvuII	CAGCTG	3 cut(s)	2108, 2939, 3188
RgaI	GCGATCGC	1 cut(s)	778
RseI	CAYNNNNRTG	2 cut(s)	570, 795
SapI	GCTCTTC	1 cut(s)	270
Sau96I	GGNCC	2 cut(s)	1191, 2299
SbfI	CCTGCAGG	1 cut(s)	1857
ScaI	AGTACT	3 cut(s)	2222, 3388, 3463
SchI	GAGTC	5 cut(s)	212, 245, 2234, 2541, 3044
ScrFI	CCNGG	1 cut(s)	3429
SdaI	CCTGCAGG	1 cut(s)	1857
SduI	GDGCHC	3 cut(s)	213, 2998, 3370
SfaAI	GCGATCGC	1 cut(s)	778
SfaNI	GCATC	8 cut(s)	48, 428, 558, 562, 700, 789, 1513, 2542
SfcI	CTRYAG	1 cut(s)	1853
SfoI	GGCGCC	1 cut(s)	295
SfuI	TTCGAA	2 cut(s)	1979, 2601
SgfI	GCGATCGC	1 cut(s)	778
SinI	GGWCC	2 cut(s)	1191, 2299
SmiMI	CAYNNNNRTG	2 cut(s)	570, 795
SmlI	CTYRAG	3 cut(s)	197, 820, 3551
SmoI	CTYRAG	3 cut(s)	197, 820, 3551
SpeI	ACTAGT	2 cut(s)	1055, 3458
Sse8387I	CCTGCAGG	1 cut(s)	1857
SsiI	CCGC	6 cut(s)	183, 291, 336, 686, 2549, 3092
SspDI	GGCGCC	1 cut(s)	293
SspI	AATATT	2 cut(s)	1974, 3088
SspMI	CTAG	7 cut(s)	692, 1056, 1299, 2343, 3353, 3459, 3540
StyD4I	CCNGG	1 cut(s)	3427
StyI	CCWWGG	1 cut(s)	1763
TaaI	ACNGT	6 cut(s)	1004, 1014, 1169, 2099, 2390, 2491
TatI	WGTACW	4 cut(s)	808, 2220, 3386, 3461
TauI	GCSGC	2 cut(s)	294, 338
TscAI	CASTG	5 cut(s)	37, 1009, 2102, 2542, 2877
TseFI	GTSAC	6 cut(s)	599, 1004, 1676, 2089, 2537, 2807
Tsp45I	GTSAC	6 cut(s)	599, 1004, 1676, 2089, 2537, 2807
TspGWI	ACGGA	1 cut(s)	367
TspRI	CASTG	5 cut(s)	37, 1009, 2102, 2542, 2877
Van91I	CCANNNNNTGG	2 cut(s)	2748, 3428
Vha464I	CTTAAG	2 cut(s)	197, 3551
VneI	GTGCAC	1 cut(s)	2994
VpaK11BI	GGWCC	2 cut(s)	1191, 2299
XapI	RAATTY	9 cut(s)	104, 560, 764, 786, 826, 2074, 2756, 3237, 3302
XceI	RCATGY	3 cut(s)	244, 857, 3136
XspI	CTAG	7 cut(s)	692, 1056, 1299, 2343, 3353, 3459, 3540
ZraI	GACGTC	1 cut(s)	1400
ZrmI	AGTACT	3 cut(s)	2222, 3388, 3463

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.