MD17G1070200.v1.1

Ribosomal RNA-processing protein 7 (RRP7)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002008
Physical Location & Seq
Forward (+)
5677958 .. 5681556
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1070200.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGAAAAAGATGGGAACGAAGGACAAGAAGAAGATCCATAAGAACAAGAAGGTTGCCCAGCCAACTTTGGAGCTTGTTGCAAAGGATAAAAAATTAGGATCTGCAGAGATTAGAAAGAGAAAAAGGGCAAAGGAAAGCGATGCTGATTCTCGAAAGGTTGTTCATGTCTCTATTGAAGTAGATAATTCAGCCAAACCAGCCAAGGATAAGAAAAAGGCAAAACTAACGAATAAAAGGAAAATAAAGAATGCGAAGAACTATGAAGCTATGGAAGGCAAGAATGATCATCCAAATACAGACACTGATGACGATTTTCATGAAATAAATGATGATGGAGTTGCCGATCAGAGCCCATTTGAAACTCAGGAAGAGATTGAAATTGGTGAAGTGCTCACTGAGGCTGGTAAGTCAAAGAAAGCTAAGAAAAAGAAAAAGAAGGATCGTAATTCATTGGAGTTTGTGAAGTCCCTTGACGAGGAAGTAGCTGGTCAGGAGAAGAGCTCAAAAGGGATATCAGGTGTACCCAAGAAAGCTTCAAGAAAGAAAAAGAAGGATCTTGATTCGTCAAAGTCTAAGAAGACACTGGAAAGGGAAGTAGAAGCTGATCAGGCTGATGTTTACCTTATTTCTTCTGGGGATGAGGACTCTTCGAAAGGAATGAAAAAATGGATTATGGAATACCATCAAAGTAGACCTGGGTTGAAGGTACTGCAGCAAAGAATTGATCAGTTTATGGTTGAACATGATGAAAAACTGGAACAGGAAAAGAAAGAGAAAGAAGCCCGTGCTGCAGACGGAGGATGGACAGTTGTTGTACATCATAAAGGAAGGAAGAAGACCACGGATTCTGAGAGTGGAATTACAGTAGGATCTGTTGCCCAGGCTGCTTTGGAGGACAAGGTGGCCAAGAAGAAGCGCACAGAAGTTGTTGGGCTAGATTTTTACCGGTTCCAAAGGAAAGAAGCTCAGAGAAACGAAATTATGATGCTCCAGAGCAAATTTGAGCAAGCTAAAAAGCGGATACAGCAGTTAAGAGCGGCTAGGAAGTTTCGACCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RRP7

Protein Analysis

354

Amino Acids

40.28

Weight (kDa)

9.71

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP7 PF12923 229 - 353 9.6e-26 Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1037
AccI GTMKAC 1 cut(s) 691
AciI CCGC 2 cut(s) 1018, 1037
AclWI GGATC 5 cut(s) 28, 106, 447, 561, 877
AcoI YGGCCR 1 cut(s) 903
AcsI RAATTY 1 cut(s) 998
AfaI GTAC 3 cut(s) 522, 708, 816
AfiI CCNNNNNNNGG 1 cut(s) 475
AgeI ACCGGT 1 cut(s) 945
AgsI TTSAA 6 cut(s) 176, 359, 377, 537, 703, 740
AjnI CCWGG 2 cut(s) 694, 879
AluBI AGCT 9 cut(s) 73, 266, 419, 485, 501, 533, 602, 965, 1010
AluI AGCT 9 cut(s) 73, 266, 419, 485, 501, 533, 602, 965, 1010
Alw21I GWGCWC 2 cut(s) 393, 503
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 5 cut(s) 28, 106, 447, 561, 877
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 1 cut(s) 903
ApeKI GCWGC 3 cut(s) 712, 788, 884
ApoI RAATTY 1 cut(s) 998
AsiGI ACCGGT 1 cut(s) 945
AspLEI GCGC 1 cut(s) 918
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 650
BaeI ACNNNNGTAYC 2 cut(s) 1013, 1046
BalI TGGCCA 1 cut(s) 905
BanII GRGCYC 2 cut(s) 353, 503
BbsI GAAGAC 2 cut(s) 584, 842
Bbv12I GWGCWC 2 cut(s) 393, 503
BbvI GCAGC 3 cut(s) 724, 775, 871
BccI CCATC 4 cut(s) 4, 326, 690, 795
BciT130I CCWGG 2 cut(s) 696, 881
BciVI GTATCC 1 cut(s) 1014
BclI TGATCA 3 cut(s) 283, 604, 724
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 2 cut(s) 935, 1041
BfmI CTRYAG 3 cut(s) 102, 710, 789
BfuI GTATCC 1 cut(s) 1014
BisI GCNGC 4 cut(s) 713, 789, 885, 1038
BlsI GCNGC 4 cut(s) 714, 790, 886, 1039
Bme1390I CCNGG 2 cut(s) 696, 881
BmiI GGNNCC 1 cut(s) 950
BmrFI CCNGG 2 cut(s) 696, 881
BmsI GCATC 2 cut(s) 130, 975
BpiI GAAGAC 2 cut(s) 584, 842
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 974
Bpu14I TTCGAA 1 cut(s) 650
BsaJI CCNNGG 4 cut(s) 201, 695, 840, 879
BsaWI WCCGGW 1 cut(s) 945
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse118I RCCGGY 1 cut(s) 945
Bse1I ACTGG 2 cut(s) 588, 759
BseBI CCWGG 2 cut(s) 696, 881
BseDI CCNNGG 4 cut(s) 201, 695, 840, 879
BseGI GGATG 3 cut(s) 286, 643, 806
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 4 cut(s) 377, 387, 840, 980
BseNI ACTGG 2 cut(s) 588, 759
BseXI GCAGC 3 cut(s) 724, 775, 871
BseYI CCCAGC 1 cut(s) 57
BshFI GGCC 1 cut(s) 905
BshTI ACCGGT 1 cut(s) 945
BsiHKAI GWGCWC 2 cut(s) 393, 503
BsiSI CCGG 1 cut(s) 946
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 1 cut(s) 905
Bsp119I TTCGAA 1 cut(s) 650
Bsp1286I GDGCHC 3 cut(s) 353, 393, 503
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
BspACI CCGC 2 cut(s) 1018, 1037
BspANI GGCC 1 cut(s) 905
BspCNI CTCAG 4 cut(s) 376, 388, 841, 979
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 950
BspMAI CTGCAG 3 cut(s) 106, 714, 793
BspPI GGATC 5 cut(s) 28, 106, 447, 561, 877
BspQI GCTCTTC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 650
BsrBI CCGCTC 1 cut(s) 1037
BsrFI RCCGGY 1 cut(s) 945
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 2 cut(s) 588, 759
BssAI RCCGGY 1 cut(s) 945
BssECI CCNNGG 4 cut(s) 201, 695, 840, 879
BssMI GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 696, 881
Bst4CI ACNGT 2 cut(s) 808, 865
Bst6I CTCTTC 3 cut(s) 363, 491, 652
BstAUI TGTACA 1 cut(s) 814
BstBI TTCGAA 1 cut(s) 650
BstC8I GCNNGC 1 cut(s) 1008
BstDEI CTNAG 6 cut(s) 363, 396, 420, 573, 849, 966
BstDSI CCRYGG 1 cut(s) 840
BstENI CCTNNNNNAGG 1 cut(s) 473
BstF5I GGATG 3 cut(s) 286, 643, 806
BstHHI GCGC 1 cut(s) 918
BstKTI GATC 9 cut(s) 36, 101, 286, 346, 442, 556, 607, 727, 872
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
BstMWI GCNNNNNNNGC 5 cut(s) 197, 608, 788, 884, 1024
BstNI CCWGG 2 cut(s) 696, 881
BstSCI CCNGG 2 cut(s) 694, 879
BstSFI CTRYAG 3 cut(s) 102, 710, 789
BstV1I GCAGC 3 cut(s) 724, 775, 871
BstV2I GAAGAC 2 cut(s) 584, 842
BstX2I RGATCY 4 cut(s) 33, 98, 553, 869
BstYI RGATCY 4 cut(s) 33, 98, 553, 869
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 1 cut(s) 905
BtgI CCRYGG 1 cut(s) 840
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 286, 643, 806
BtsIMutI CAGTG 3 cut(s) 300, 393, 581
Cac8I GCNNGC 1 cut(s) 1008
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 918
Cfr10I RCCGGY 1 cut(s) 945
Csp6I GTAC 3 cut(s) 521, 707, 815
CspAI ACCGGT 1 cut(s) 945
CviAII CATG 3 cut(s) 164, 317, 743
CviQI GTAC 3 cut(s) 521, 707, 815
DdeI CTNAG 6 cut(s) 363, 396, 420, 573, 849, 966
DpnI GATC 9 cut(s) 35, 100, 285, 345, 441, 555, 606, 726, 871
DpnII GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
EaeI YGGCCR 1 cut(s) 903
Eam1104I CTCTTC 3 cut(s) 363, 491, 652
EarI CTCTTC 3 cut(s) 363, 491, 652
Ecl136II GAGCTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 353, 503
Eco32I GATATC 1 cut(s) 513
Eco53kI GAGCTC 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 501
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 694, 879
EcoRV GATATC 1 cut(s) 513
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 353, 503
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 3 cut(s) 167, 320, 746
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 163, 316, 742
FbaI TGATCA 3 cut(s) 283, 604, 724
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 713, 789, 885, 1038
FokI GGATG 3 cut(s) 273, 650, 813
FriOI GRGCYC 2 cut(s) 353, 503
Fsp4HI GCNGC 4 cut(s) 713, 789, 885, 1038
FspBI CTAG 2 cut(s) 935, 1041
GlaI GCGC 1 cut(s) 917
GluI GCNGC 4 cut(s) 713, 789, 885, 1038
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 974
HaeIII GGCC 1 cut(s) 905
HapII CCGG 1 cut(s) 946
HhaI GCGC 1 cut(s) 918
Hin1II CATG 3 cut(s) 167, 320, 746
Hin6I GCGC 1 cut(s) 916
HinP1I GCGC 1 cut(s) 916
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 4 cut(s) 146, 560, 644, 845
HpaII CCGG 1 cut(s) 946
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 521, 619, 692
Hpy188I TCNGA 3 cut(s) 348, 850, 969
Hpy188III TCNNGA 7 cut(s) 150, 317, 365, 491, 537, 557, 991
Hpy8I GTNNAC 3 cut(s) 521, 619, 692
HpyAV CCTTC 7 cut(s) 13, 43, 266, 430, 544, 697, 822
HpyCH4III ACNGT 2 cut(s) 808, 865
HpyCH4V TGCA 4 cut(s) 80, 104, 712, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 608, 788, 884, 1024
HpyF3I CTNAG 6 cut(s) 363, 396, 420, 573, 849, 966
Hsp92II CATG 3 cut(s) 167, 320, 746
HspAI GCGC 1 cut(s) 916
Ksp22I TGATCA 3 cut(s) 283, 604, 724
Kzo9I GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 2 cut(s) 70, 993
Lsp1109I GCAGC 3 cut(s) 724, 775, 871
LweI GCATC 2 cut(s) 130, 975
MaeI CTAG 2 cut(s) 935, 1041
MalI GATC 9 cut(s) 35, 100, 285, 345, 441, 555, 606, 726, 871
MbiI CCGCTC 1 cut(s) 1037
MboI GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
MflI RGATCY 4 cut(s) 33, 98, 553, 869
MhlI GDGCHC 3 cut(s) 353, 393, 503
MlsI TGGCCA 1 cut(s) 905
MluCI AATT 8 cut(s) 92, 184, 378, 445, 720, 858, 978, 998
MluNI TGGCCA 1 cut(s) 905
MlyI GAGTC 1 cut(s) 638
MnlI CCTC 5 cut(s) 391, 469, 634, 791, 886
Mox20I TGGCCA 1 cut(s) 905
MscI TGGCCA 1 cut(s) 905
MseI TTAA 1 cut(s) 1031
Msp20I TGGCCA 1 cut(s) 905
MspI CCGG 1 cut(s) 946
MspR9I CCNGG 2 cut(s) 696, 881
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 2 cut(s) 696, 881
MwoI GCNNNNNNNGC 5 cut(s) 197, 608, 788, 884, 1024
NdeII GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
NlaIII CATG 3 cut(s) 167, 320, 746
NlaIV GGNNCC 1 cut(s) 950
NspV TTCGAA 1 cut(s) 650
PagI TCATGA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 491
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 146, 560, 845
PinAI ACCGGT 1 cut(s) 945
PkrI GCNGC 4 cut(s) 714, 790, 886, 1039
PleI GAGTC 1 cut(s) 638
PpsI GAGTC 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 694, 879
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 2 cut(s) 694, 879
PspN4I GGNNCC 1 cut(s) 950
PstI CTGCAG 3 cut(s) 106, 714, 793
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 4 cut(s) 33, 98, 553, 869
RsaI GTAC 3 cut(s) 522, 708, 816
RsaNI GTAC 3 cut(s) 521, 707, 815
SacI GAGCTC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 1 cut(s) 1031
SatI GCNGC 4 cut(s) 713, 789, 885, 1038
Sau3AI GATC 9 cut(s) 33, 98, 283, 343, 439, 553, 604, 724, 869
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 2 cut(s) 696, 881
SduI GDGCHC 3 cut(s) 353, 393, 503
SfaNI GCATC 2 cut(s) 130, 975
SfcI CTRYAG 3 cut(s) 102, 710, 789
SfuI TTCGAA 1 cut(s) 650
Sse9I AATT 8 cut(s) 92, 184, 378, 445, 720, 858, 978, 998
SsiI CCGC 2 cut(s) 1018, 1037
SspMI CTAG 2 cut(s) 935, 1041
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 2 cut(s) 694, 879
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 808, 865
TaqI TCGA 3 cut(s) 151, 650, 1051
TasI AATT 8 cut(s) 92, 184, 378, 445, 720, 858, 978, 998
TatI WGTACW 1 cut(s) 814
TauI GCSGC 1 cut(s) 1040
TfiI GAWTC 3 cut(s) 146, 560, 845
Tru1I TTAA 1 cut(s) 1031
Tru9I TTAA 1 cut(s) 1031
TscAI CASTG 3 cut(s) 307, 400, 588
TseI GCWGC 3 cut(s) 712, 788, 884
TspDTI ATGAA 8 cut(s) 17, 152, 276, 305, 333, 438, 674, 762
TspGWI ACGGA 2 cut(s) 810, 857
TspRI CASTG 3 cut(s) 307, 400, 588
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 2 cut(s) 935, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.